11 results on '"hair follicle cell proliferation"'
Search Results
2. ERK1/2 Pathway Is Involved in the Enhancement of Fatty Acids from Phaeodactylum tricornutum Extract (PTE) on Hair Follicle Cell Proliferation
- Author
-
Zhiyi Chen, Bing Li, Yuting Li, Xia Zhang, Lin Li, and Lei Xiao
- Subjects
chemistry.chemical_classification ,Article Subject ,integumentary system ,General Immunology and Microbiology ,Cell growth ,Linoleic acid ,General Medicine ,Outer root sheath ,Hair follicle ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Melanin ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Docosahexaenoic acid ,medicine ,Medicine ,sense organs ,Hair follicle cell proliferation ,Polyunsaturated fatty acid - Abstract
Extractions from Phaeodactylum tricornutum have been widely studied and evaluated to various biological effects. The aim of this study was to investigate the promotional effect of P. tricornutum extract (PTE) on the ERK1/2 signaling pathway involved in hair follicle cell proliferation. In order to illuminate the enhancement of PTE on hair growth by promoting proliferation of hair follicle cells, the activities of human hair follicle outer root sheath cell (HFORSC), human hair follicle germinal matrix cells (HFGMC), and hair epithelial melanocytes (HEM) were observed under PET treatment. Levels of keratins, PKCζ, ERK1/2, and p38 MAPK in hair follicle cells were determined by Western blotting to illustrate the mechanisms of PTE effects on hair growth. Analyzed by GC-MS, the main polyunsaturated fatty acids which were 9.43% of total fatty acids in PTE were linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid. Melanin content and tyrosinase activity in HEM were measured. The results showed that PTE exhibited remarkable enhancement on cell proliferation. Melanin production was inhibited by PTE treatment, while keratin-14, keratin-15, and keratin-17 levels on hair follicle cells were elevated at different concentrations. The promotions of ERK1/2 and p38 MAPK levels indicated that the ERK1/2 signaling pathway is involved in the proliferation of hair follicle cells. These results are the evidence that PTE potentially deserves further study as a new natural candidate for hair care applications.
- Published
- 2020
3. Effects of photoperiod on circadian clock genes in skin contribute to the regulation of hair follicle cycling of Inner Mongolia white cashmere goats
- Author
-
Shengli Li, Chun Hua Zhang, Hai Zhou Sun, Lu Jin, Chong Zhi Zhang, and Dan Sang
- Subjects
Time Factors ,Photoperiod ,Period (gene) ,Circadian clock ,CLOCK Proteins ,Gene Expression ,Biology ,Andrology ,03 medical and health sciences ,Circadian Clocks ,Skin Physiological Phenomena ,medicine ,Animals ,Cashmere goat ,RNA, Messenger ,Circadian rhythm ,Cell Proliferation ,Skin ,030304 developmental biology ,photoperiodism ,0303 health sciences ,Goats ,Cell Cycle ,0402 animal and dairy science ,ARNTL Transcription Factors ,04 agricultural and veterinary sciences ,General Medicine ,Hair follicle ,040201 dairy & animal science ,medicine.anatomical_structure ,Hair follicle cell proliferation ,General Agricultural and Biological Sciences ,Hair Follicle ,PER1 - Abstract
This study investigated how the effects of photoperiod on circadian clock genes in the skin contribute to the regulation of hair follicle cycling of Inner Mongolia white cashmere goats. Twenty-four female (non-pregnant) Inner Mongolia white cashmere goats aged 1 - 1.5 years old with similar live weights (mean, 20.36 ± 2.63 kg) were randomly allocated into two groups: a natural daily photoperiod group (NDPP group:10 - 16 hr Light, n = 12) and a short daily photoperiod group (SDPP group: 7 hr Light:17 hr Dark, n = 12). All goats were housed in individual pens from May 15 to October 15, 2015 and were fed the same diets. We detected the mRNA expression of brain and muscle arnt-like protein-1 (Bmal1), circadian locomotor output control kaput (Clock), cryptochrome-1 (Cry1), period homolog-1 (Per1) and Rev-erbα genes in the goat skin. ANOVA revealed a significant 24 hr (10:00 hr, 14:00 hr, 18:00 hr, 22:00 hr, 02:00 hr, 06:00 hr, 10:00 hr) variation between the SDPP and NDPP groups for three months (July, September, and October). In summary, the current results confirm that an intrinsic oscillating molecular clock exists in goat skin, and that the clock is important for potential timing mechanisms at the anagen phase of hair follicles, which would contribute to the regulation mechanisms of hair follicle cell proliferation.
- Published
- 2019
4. Hot water extract of oriental melon leaf promotes hair growth and prolongs anagen hair cycle: In vivo and in vitro evaluation
- Author
-
Long-Quan Pi, Won-Soo Lee, and Sung Hee Min
- Subjects
integumentary system ,Biology ,Hair follicle ,Organ culture ,medicine.disease ,Applied Microbiology and Biotechnology ,Article ,Bulb ,Andrology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Hair loss ,In vivo ,Hair cycle ,030220 oncology & carcinogenesis ,Botany ,otorhinolaryngologic diseases ,medicine ,Human hair growth ,sense organs ,Hair follicle cell proliferation ,Food Science ,Biotechnology - Abstract
Effects of hot water extracts of oriental melon leaves (OML) on promotion of hair growth were investigated. Topical OML extract administration for hair regeneration was investigated using an in vivo model with C57BL/6 mice. Effects of OML extracts on the human hair growth were investigated using a hair follicle organ culture. OML extracts induced a shortened telogen to anagen conversion and promoted hair growth in the C57BL/6 mouse model. Culture of human hair follicles in the presence of OML extracts for 8 days promoted hair growth and prolonged the anagen duration due to induction of hair follicle cell proliferation in the bulb region. OML extracts exert a hair growth promotion effect and, therefore, can be used as a therapeutic agent for prevention of hair loss.
- Published
- 2016
5. Regulations of Reversal of Senescence by PKC Isozymes in Response to 12-O-Tetradecanoylphorbol-13-Acetate via Nuclear Translocation of pErk1/2
- Author
-
Yun Yeong Lee, Min Sook Ryu, Masami Suganuma, Kye Yong Song, In Kyoung Lim, and Hong Seok Kim
- Subjects
0301 basic medicine ,Senescence ,Protein Kinase C-alpha ,MAP Kinase Signaling System ,HDF ,PKCβ1 ,Biology ,12-O-Tetradecanoylphorbol-13-acetate ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Protein Kinase C beta ,Animals ,Humans ,Kinase activity ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Protein kinase C ,Cellular Senescence ,Cell Proliferation ,Genetics ,SA-pErk1/2 ,integumentary system ,PKCα ,tumor promotion ,Cell Biology ,General Medicine ,Fibroblasts ,Cell biology ,Protein Transport ,030104 developmental biology ,chemistry ,Gene Expression Regulation ,Tetradecanoylphorbol Acetate ,Hair follicle cell proliferation ,Cell aging - Abstract
The mechanism by which 12-O-tetradecanoylphorbol-13-acetate (TPA) bypasses cellular senescence was investigated using human diploid fibroblast (HDF) cell replicative senescence as a model. Upon TPA treatment, protein kinase C (PKC) α and PKCβ1 exerted differential effects on the nuclear translocation of cytoplasmic pErk1/2, a protein which maintains senescence. PKCα accompanied pErk1/2 to the nucleus after freeing it from PEA-15pS(104) via PKCβ1 and then was rapidly ubiquitinated and degraded within the nucleus. Mitogen-activated protein kinase docking motif and kinase activity of PKCα were both required for pErk1/2 transport to the nucleus. Repetitive exposure of mouse skin to TPA downregulated PKCα expression and increased epidermal and hair follicle cell proliferation. Thus, PKCα downregulation is accompanied by in vivo cell proliferation, as evidenced in 7, 12-dimethylbenz(a)anthracene (DMBA)-TPA-mediated carcinogenesis. The ability of TPA to reverse senescence was further demonstrated in old HDF cells using RNA-sequencing analyses in which TPA-induced nuclear PKCα degradation freed nuclear pErk1/2 to induce cell proliferation and facilitated the recovery of mitochondrial energy metabolism. Our data indicate that TPA-induced senescence reversal and carcinogenesis promotion share the same molecular pathway. Loss of PKCα expression following TPA treatment reduces pErk1/2-activated SP1 biding to the p21(WAF1) gene promoter, thus preventing senescence onset and overcoming G1/S cell cycle arrest in senescent cells.
- Published
- 2016
6. Comparative Evaluation of the Clinical Efficacy of PRP-Therapy, Minoxidil, and Their Combination with Immunohistochemical Study of the Dynamics of Cell Proliferation in the Treatment of Men with Androgenetic Alopecia
- Author
-
Irina O. Smirnova and Elena E. Pakhomova
- Subjects
Male ,platelet-rich plasma (PRP) ,animal diseases ,Biopsy ,minoxidil ,CD34 ,Cell Count ,Pharmacology ,lcsh:Chemistry ,030207 dermatology & venereal diseases ,0302 clinical medicine ,lcsh:QH301-705.5 ,Spectroscopy ,androgenetic alopecia (AGA) ,integumentary system ,biology ,medicine.diagnostic_test ,Platelet-Rich Plasma ,General Medicine ,Combined Modality Therapy ,Immunohistochemistry ,Computer Science Applications ,hair morphometry ,Treatment Outcome ,medicine.anatomical_structure ,Minoxidil ,030220 oncology & carcinogenesis ,Hair follicle cell proliferation ,Antibody ,Hair Follicle ,Ki67 ,medicine.drug ,Adult ,Dkk-1 ,Platelet Transfusion ,Article ,Catalysis ,Inorganic Chemistry ,Young Adult ,03 medical and health sciences ,medicine ,Humans ,hair follicle cell proliferation ,Physical and Theoretical Chemistry ,Molecular Biology ,Cell Proliferation ,Scalp ,Cell growth ,business.industry ,Organic Chemistry ,Alopecia ,β-catenin ,Hair follicle ,nervous system diseases ,lcsh:Biology (General) ,lcsh:QD1-999 ,Skin biopsy ,biology.protein ,business ,Hair - Abstract
Platelet-rich plasma (PRP) therapy has been considered as a promising treatment for androgenetic alopecia (AGA). The aim of the study was comparative evaluation of the clinical efficacy of PRP-therapy, minoxidil, and their combination in the treatment of men with AGA and to evaluate the effects of PRP on the proliferation of hair follicle (HF) cells in skin biopsy. Materials and Methods: The study involved 69 men who were divided into 3 groups who received PRP therapy, minoxidil, and their combination. The clinical efficacy of the therapy was evaluated by the dynamics of morphometric of hairs. To assess cell proliferation antibodies to &beta, catenin, CD34, Ki67, and to Dkk-1 were used. Results. PRP treatment was more effective than minoxidil therapy (p = 0.005). Complex therapy turned out to be more effective than minoxidil monotherapy (p <, 0.0001) and PRP monotherapy (p = 0.007). After applying PRP the absolute and relative values of the &beta, catenin and CD34 expression area increased, an increase in Ki67+ index was also significant. Conclusions: PRP can be considered as a treatment option for AGA. Combined PRP and minoxidil use seems promising for the treatment of AGA. PRP increase in the proliferative activity of HF cells and improves hair morphology in patients with AGA.
- Published
- 2020
7. Comparative Evaluation of the Clinical Efficacy of PRP-Therapy, Minoxidil, and Their Combination with Immunohistochemical Study of the Dynamics of Cell Proliferation in the Treatment of Men with Androgenetic Alopecia.
- Author
-
Pakhomova, Elena E. and Smirnova, Irina O.
- Subjects
- *
MINOXIDIL , *CELL proliferation , *HAIR follicles , *PLATELET-rich plasma , *BALDNESS , *HAIR cells - Abstract
Platelet-rich plasma (PRP) therapy has been considered as a promising treatment for androgenetic alopecia (AGA). The aim of the study was comparative evaluation of the clinical efficacy of PRP-therapy, minoxidil, and their combination in the treatment of men with AGA and to evaluate the effects of PRP on the proliferation of hair follicle (HF) cells in skin biopsy. Materials and Methods: The study involved 69 men who were divided into 3 groups who received PRP therapy, minoxidil, and their combination. The clinical efficacy of the therapy was evaluated by the dynamics of morphometric of hairs. To assess cell proliferation antibodies to β-catenin, CD34, Ki67, and to Dkk-1 were used. Results. PRP treatment was more effective than minoxidil therapy (p = 0.005). Complex therapy turned out to be more effective than minoxidil monotherapy (p < 0.0001) and PRP monotherapy (p = 0.007). After applying PRP the absolute and relative values of the β-catenin and CD34 expression area increased; an increase in Ki67+ index was also significant. Conclusions: PRP can be considered as a treatment option for AGA. Combined PRP and minoxidil use seems promising for the treatment of AGA. PRP increase in the proliferative activity of HF cells and improves hair morphology in patients with AGA. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
8. Cyclosporine A stimulated hair growth from mouse vibrissae follicles in an organ culture model
- Author
-
Kun Yao, Weixin Fan, and Wenrong Xu
- Subjects
Hypertrichosis ,medicine.medical_specialty ,mouse vibrissae ,Organ culture ,cyclosporine A (CsA) ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Internal medicine ,medicine ,biology ,integumentary system ,hair follicle ,General Medicine ,medicine.disease ,Hair follicle ,Vascular endothelial growth factor ,Nerve growth factor ,medicine.anatomical_structure ,Endocrinology ,chemistry ,biology.protein ,Hepatocyte growth factor ,Hair follicle cell proliferation ,Follistatin ,medicine.drug ,Research Paper - Abstract
Hypertrichosis is one of the most common side effects of systemic cyclosporine A therapy. It has been previously shown that cyclosporine A induces anagen and inhibits catagen development in mice. In the present study, to explore the mechanisms of cyclosporine A, we investigated the effects of cyclosporine A on hair shaft elongation, hair follicle cell proliferation, apoptosis, and mRNA expression of selected growth factors using an organ culture model of mouse vibrissae. In this model, cyclosporine A stimulated hair growth of normal mouse vibrissae follicles by inhibiting catagen-like development and promoting matrix cell proliferation. In addition, cyclosporine A caused an increase in the expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and nerve growth factor (NGF), and inhibited follistatin expression. Our findings provide an explanation for the clinically observed effects of cyclosporine A on hair growth. The mouse vibrissae organ culture offers an attractive model for identifying factors involved in the modulation of hair growth.
- Published
- 2011
9. Regulation of Hair Follicle Development: An In Vitro Model for Hair Follicle Invasion of Dermis and Associated Connective Tissue Remodeling
- Author
-
Qizhi Wang, Wendy C. Weinberg, Steven R. Ledbetter, Thomas P. Dooley, Linda V. Goodman, Ulrike Lichti, and Stuart H. Yuspa
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Dermatology ,Biology ,Biochemistry ,Mice ,Follicle ,Dermis ,Epidermal growth factor ,Internal medicine ,medicine ,Animals ,Collagenases ,Growth Substances ,Molecular Biology ,Cells, Cultured ,Mice, Inbred BALB C ,integumentary system ,Growth factor ,3T3 Cells ,Cell Biology ,Hair follicle ,Cell biology ,Enzyme Activation ,Dermal papillae ,medicine.anatomical_structure ,Endocrinology ,Epidermal Cells ,Connective Tissue ,Collagenase ,Hair follicle cell proliferation ,Hair ,medicine.drug - Abstract
During embryonic development presumptive hair follicle cells of epithelial and mesenchymal origin are determined in defined body locations. This is followed by rapid proliferation of epithelial cells and associated penetration into the dermis in response to as yet undetermined signals. A collagen matrix culture system, which maintains the three-dimensional relationships of hair follicle cells to each other, was developed to study the regulation of the enlargement of immature hair follicles and the accompanying remodeling of the dermis. In studies with a heterogeneous dermis-derived preparation of murine hair follicles, ranging in size from the earliest down-growing budding cell mass to hair-forming follicles, we had previously shown that cell proliferation was stimulated by cholera toxin and epidermal growth factor, but only the epidermal growth factor-stimulated proliferation was accompanied by digestion of the collagen matrix due to release of collagenolytic enzymes. Further studies revealed that transforming growth factor-alpha also stimulated hair follicle cell proliferation and collagenase release. However, although transforming growth factor-beta inhibited the transforming growth factor-alpha-stimulated proliferation, it enhanced the release and activation of collagenases and other gelatin-degrading enzymes detectable by gelatin zymography. Stimulation of collagenolytic activity depended on the three-dimensional hair follicle structure and did not occur in monolayer cultures of hair follicle cells. Comparison of hair follicle buds with more developed dermis-derived hair follicles, plated at the same cell density (based on DNA content), suggested that a greater fraction of cells in the bud-stage follicle responded to the growth factors by release of collagenases. Possibly only the cells in the advancing portion of growing hair follicles that are closest to the dermal papilla cell cluster produce the collagenases in response to growth factors. To examine the participation of dermal papilla cells in collagenase release and activation, several immortalized rat whisker dermal papilla cell lines were co-cultured with mouse hair follicle buds. Co-culture resulted in a marked enlargement of follicles as well as activation of the 92-kDa type IV collagenase, produced by hair follicle buds, that correlated with ability of the dermal papilla cells to stimulate hair formation in grafts of hair follicle buds on nude mice. Dermal papilla cells cultured alone produced the 72-kDa type IV collagenase, which was also activated during co-culture with hair follicle buds. Thus, two activities, both relevant for hair follicle development, namely, cell proliferation and release and activation of collagenases, have been stimulated in immature hair follicle buds by either growth-factor supplementation or interaction with dermal papilla cells.(ABSTRACT TRUNCATED AT 400 WORDS)
- Published
- 1993
10. Akt2 and SGK3 are both determinants of postnatal hair follicle development
- Author
-
Jian Wang, Krishna M. Boini, Florian Lang, Bobby R. Monks, David A. Pearce, Leena Ray, James A. McCormick, Theodora M. Mauro, and Morris J. Birnbaum
- Subjects
Keratinocytes ,medicine.medical_specialty ,Morphogenesis ,AKT1 ,Biology ,Protein Serine-Threonine Kinases ,Biochemistry ,AKT3 ,Research Communications ,Follicle ,Mice ,Sebaceous Glands ,Internal medicine ,Genetics ,medicine ,Animals ,Molecular Biology ,Gene knockout ,beta Catenin ,Cell Proliferation ,Mice, Knockout ,integumentary system ,Age Factors ,Hair follicle ,Cell biology ,medicine.anatomical_structure ,Hair follicle morphogenesis ,Endocrinology ,embryonic structures ,Hair follicle cell proliferation ,Hair Follicle ,Proto-Oncogene Proteins c-akt ,Biotechnology - Abstract
SGK3, which previously has been shown to play a key role in hair follicle development in mice, is a member of the AGC family of serine-threonine kinases. Mice lacking SGK3 have abnormal follicle cycling, which begins shortly after birth and ameliorates substantially with age. However, this developmental abnormality is not recapitulated in mice lacking closely related kinases Akt1, Akt2, or Akt3. To examine whether Akt2 interacts with SGK3 in postnatal hair development, we have generated and characterized Akt2/SGK3 double knockouts (DKOs). We find that the DKO mice have a defect in hair growth that is markedly worse than that of SGK3−/− mice and does not ameliorate with age. Morphologically, this defect is characterized by accelerated entry into catagen and through anagen, irregular hair follicle orientation, and increased expression of sebaceous glands. The defect is preceded by a profound failure to increase follicle matrix cell nuclear β-catenin accumulation and proliferation at the onset of morphogenesis. Furthermore, in cultured keratinocytes, transfected Akt2 and SGK3 both stimulate transcription of a β-catenin-LEF1-dependent reporter gene. Thus, SGK3 and Akt2 both appear to play important roles in postnatal hair follicle morphogenesis, likely because of their redundant regulation of β-catenin-dependent transcriptional processes, which control hair follicle cell proliferation.—Mauro, T. M., McCormick, J. A., Wang, J., Boini, K. M., Ray, L., Monks, B., Birnbaum, M. J., Lang, F., and Pearce, D. Akt2 and SGK3 are both determinants of postnatal hair follicle development.
- Published
- 2009
11. Epidermal Stem Cells Cultured on Collagen-Modified Chitin Membrane Induce In Situ Tissue Regeneration of Full-Thickness Skin Defects in Mice
- Author
-
Rong Liang, Jiake Xu, Weiguo Liang, Honghui Chen, Shirley Mei, Zhihe Liu, Xiaojian Li, Zhi Zhang, Libing Dai, Wenjuan Cao, Shennan Qin, Guangxiong Guan, and Yan Shen
- Subjects
Skin Physiology ,Pathology ,Anatomy and Physiology ,Mouse ,lcsh:Medicine ,Chitin ,Stem cell marker ,Mice ,Engineering ,Biomimetics ,Skin Physiological Phenomena ,Molecular Cell Biology ,lcsh:Science ,reproductive and urinary physiology ,Skin ,Burn Management ,Multidisciplinary ,integumentary system ,Stem Cells ,Skin Transplantation ,Animal Models ,Cell biology ,medicine.anatomical_structure ,Transplant Surgery ,embryonic structures ,Medicine ,Cellular Types ,Hair follicle cell proliferation ,Stem cell ,Research Article ,Biotechnology ,medicine.medical_specialty ,Trauma Surgery ,Biomedical Engineering ,Bioengineering ,Biology ,Biomaterials ,Model Organisms ,medicine ,Animals ,Regeneration ,Skin, Artificial ,Wound Healing ,Tissue Engineering ,Regeneration (biology) ,lcsh:R ,Reconstructive Surgery ,Transplantation ,lcsh:Q ,Surgery ,Epidermis ,Wound healing ,Stem Cell Transplantation ,Developmental Biology - Abstract
A Large scale of full-thickness skin defects is lack of auto-grafts and which requires the engineered skin substitutes for repair and regeneration. One major obstacle in skin tissue engineering is to expand epidermal stem cells (ESCs) and develop functional substitutes. The other one is the scaffold of the ESCs. Here, we applied type I collagen-modified chitin membrane to form collagen-chitin biomimetic membrane (C-CBM), which has been proved to have a great biocompatibility and degraded totally when it was subcutaneously transplanted into rat skin. ESCs were cultured, and the resulting biofilm was used to cover full-thickness skin defects in nude mice. The transplantation of ESCs- collagen- chitn biomimetic membrane (ESCs-C-CBM) has achieved in situ skin regeneration. In nude mice, compared to controls with collagen-chitin biomimetic membrane (C-CBM) only, the ESCs-C-CBM group had significantly more dermatoglyphs on the skin wound 10 w after surgery, and the new skin was relatively thick, red and elastic. In vivo experiments showed obvious hair follicle cell proliferation in the full-thickness skin defect. Stem cell markers examination showed active ESCs in repair and regeneration of skin. The results indicate that the collagen-modified chitin membrane carry with ESCs has successfully regenerated the whole skin with all the skin appendages and function.
- Published
- 2014
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.