21 results on '"de la Garza-Ramos, Myriam Angélica"'
Search Results
2. Molecular detection of Helicobacter pylori based on the presence of cagA and vacA virulence genes in dental plaque from patients with periodontitis
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Flores-Treviño, Carlos Eduardo, Urrutia-Baca, Víctor Hugo, Gómez-Flores, Ricardo, De La Garza-Ramos, Myriam Angélica, Sánchez-Chaparro, María Marisela, and Garza-Elizondo, Mario Alberto
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- 2019
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3. Effect of Culture Conditions of Lophocereus marginatus Endophytic Fungi on Yield and Anticancer and Antioxidant Activities
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Ramírez-Villalobos, Jesica María, primary, Gomez-Flores, Ricardo, additional, Velázquez-Flores, Priscilla Viridiana, additional, Morán-Santibáñez, Karla Selene, additional, Tamez-Guerra, Patricia, additional, Pérez-González, Orquídea, additional, de la Garza-Ramos, Myriam Angélica, additional, Rodríguez-Padilla, Cristina, additional, and Romo-Sáenz, César Iván, additional
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- 2023
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4. Cytotoxicity of Peruvian propolis and Psidium guajava on human gingival fibroblasts, PBMCs and HeLa cells
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Millones-Gómez, Pablo Alejandro, primary, De la Garza-Ramos, Myriam Angélica, additional, Urrutia-Baca, Victor Hugo, additional, Hernandez-Martinez, Humberto Carlos, additional, Hernández Marín, David Alejandro, additional, and Minchón Medina, Carlos Alberto, additional
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- 2022
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5. Antimicrobial Effect of Calcium Hydroxide Combined with Electrolyzed Superoxidized Solution at Neutral pH on Enterococcus faecalis Growth
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Jimenez-Gonzalez, Héctor Armando, primary, Nakagoshi-Cepeda, María Argelia Akemi, additional, Nakagoshi-Cepeda, Sergio Eduardo, additional, Urrutia-Baca, Víctor Hugo, additional, De La Garza-Ramos, Myriam Angélica, additional, Solis-Soto, Juan Manuel, additional, Gomez-Flores, Ricardo, additional, and Tamez-Guerra, Patricia, additional
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- 2021
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6. Periodontitis y su Relación con el Ictus Isquémico
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Pérez-Vázquez, Gil, González-Aquines, Alejandro, Matínez-Roque, Denisse, Chávez-Luévanos, Beatriz E, de la Garza-Ramos, Myriam Angélica, and Góngora-Rivera, Fernando
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Periodontitis, infarto cerebral, ateroesclerosis, fisiopatología, salud dental - Abstract
La periodontitis se ha vinculado con el infarto cerebral (IC) y se ha descrito como un factor de riesgo para desarrollar un IC, sin embargo, la evidencia sobre su relación es controversial. El objetivo de la presente revisión es describir el impacto epidemiológico, los mecanismos fisiopatológicos y la relevancia clínica de la relación entre la enfermedad periodontal y el IC. Los estudios epidemiológicos demuestran que la periodontitis incrementa el riesgo para desarrollar un IC; efecto producido a través del estado proinflamatorio sistémico como consecuencia de la periodontitis, favoreciendo la formación de placas ateromatosas en grandes arterias. Además, el ADN bacteriano de patógenos causantes de periodontitis ha sido identificado en placas ateromatosas de pacientes con IC. Estudios recientes sugieren que el Grosor de la Íntima Media (GIM) medido por ultrasonografía está relacionado en gran medida con los factores de riesgo cardiovasculares y recientemente con la periodontitis. Por último, se ha demostrado que el tratamiento de la periodontitis reduce el riesgo de IC. La relación entre la periodontitis y el IC es evidente. No obstante, es necesario desarrollar estudios aleatorizados que permitan medir de manera más precisa su relación y que permitan implementar estrategias efectivas de detección y tratamiento oportuno de periodontitis para disminuir la carga de enfermedad que representa el ictus.
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- 2020
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7. Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH againstHelicobacter pylori
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Lucio-Sauceda, Daniela Guadalupe, primary, Urrutia-Baca, Víctor Hugo, additional, Gomez-Flores, Ricardo, additional, De La Garza-Ramos, Myriam Angélica, additional, Tamez-Guerra, Patricia, additional, and Orozco-Flores, Alonso, additional
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- 2019
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8. Immunoinformatics Approach to Design a Novel Epitope-Based Oral Vaccine AgainstHelicobacter pylori
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Urrutia-Baca, Victor Hugo, primary, Gomez-flores, Ricardo, additional, De La Garza-Ramos, Myriam Angélica, additional, Tamez-guerra, Patricia, additional, Lucio-sauceda, Daniela Guadalupe, additional, and Rodríguez-padilla, María Cristina, additional
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- 2019
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9. Immunization with a SyntheticHelicobacter pyloriPeptide Induces Secretory IgA Antibodies and Protects Mice against Infection
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Espinosa-Ramos, David, primary, Caballero-Hernández, Diana, additional, Gomez-Flores, Ricardo, additional, Trejo-Chávez, Armando, additional, Pérez-Limón, Luis Jerónimo, additional, de la Garza-Ramos, Myriam Angélica, additional, Tamez-Guerra, Reyes, additional, Tamez-Guerra, Patricia, additional, and Rodriguez-Padilla, Cristina, additional
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- 2019
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10. Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against Helicobacter pylori.
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Lucio-Sauceda, Daniela Guadalupe, Urrutia-Baca, Víctor Hugo, Gomez-Flores, Ricardo, De La Garza-Ramos, Myriam Angélica, Tamez-Guerra, Patricia, and Orozco-Flores, Alonso
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ACYLTRANSFERASES ,BACTERIAL proteins ,BIOFILMS ,BIOLOGICAL assay ,CELL lines ,CELL surface antigens ,COLORIMETRY ,FIBROBLASTS ,GENE expression ,GENTIAN violet ,GINGIVA ,HELICOBACTER diseases ,HELICOBACTER pylori ,HYDROGEN-ion concentration ,IMMUNODIAGNOSIS ,LIPOPROTEINS ,LYASES ,ORAL diseases ,MOUTHWASHES ,POLYMERASE chain reaction ,STAINS & staining (Microscopy) ,REVERSE transcriptase polymerase chain reaction - Abstract
The presence of Helicobacter pylori in the oral cavity has been associated to the failure of antimicrobial therapy in patients with gastrointestinal infection and the development of oral diseases. However, it has been reported that the maintenance of good oral hygiene can improve the therapeutic success rates, where the use of mouthwashes with anti-Helicobacter activity would help to achieve it. The aim was to evaluate the antimicrobial activity of OxOral® mouthwash against H. pylori and its effect on biofilm formation. The minimum inhibitory concentration (MIC) of OxOral® (pH = 6.4–7.5, ORP = 650–900 mV) against H. pylori was calculated testing serial dilutions 0.117–15 ppm against 1 × 10
8 CFU/mL of H. pylori (ATCC® 700824™) by broth microdilution method using 96‐well plates. The H. pylori biofilm formation was determined by the optical density measurement at 600 nm from coverslips stained with 0.1% crystal violet. The gene expression of ureA, luxS, flaA, omp18, and lpxD were analyzed by RT‐qPCR. OxOral® cytotoxicity was evaluated in a human gingival fibroblast cell line by MTT assay. MIC was of 3.75 ppm, with 99.7 ± 7.7% bacterial growth inhibition. In the negative control, the biofilm formation was observed, whereas when bacteria were treated with OxOral® at 0.234, 0.469, and 0.938 ppm, an inhibition of 35.5 ± 0.9%, 89.1 ± 1.2%, and 99.9 ± 5.5% were obtained, respectively. The gene expression analysis showed that flaA, omp18, and lpxD genes were down‐regulated with OxOral® compared with control (p < 0.05). Low cytotoxicity of 16.5 ± 7.6% was observed at the highest dose (15 ppm); no significant differences were observed from 15 to 0.469 ppm compared to the control of untreated cells (p > 0.05). Our results reveal an important anti-Helicobacter activity of OxOral® and open the possibility of its therapeutic use new studies, which would increase the success rate of conventional therapies against H. pylori. [ABSTRACT FROM AUTHOR]- Published
- 2019
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11. Immunoinformatics Approach to Design a Novel Epitope-Based Oral Vaccine Against Helicobacter pylori.
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Urrutia-Baca, Victor Hugo, Gomez-flores, Ricardo, De La Garza-Ramos, Myriam Angélica, Tamez-guerra, Patricia, Lucio-sauceda, Daniela Guadalupe, and Rodríguez-padilla, María Cristina
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- 2019
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12. A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of α-Solanine on Rat Bone Marrow Mesenchymal Stem Cells
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Ordóñez-Vásquez, Adriana, primary, Jaramillo-Gómez, Lorenza, additional, Duran-Correa, Camilo, additional, Escamilla-García, Erandi, additional, De la Garza-Ramos, Myriam Angélica, additional, and Suárez-Obando, Fernando, additional
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- 2017
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13. Inhibición de la expresión del sistema agr de Staphylococcus aureus resistente a meticilina mediante el uso de polifenoles totales de hojas de aguacate mexicano (Persea americana var. drymifolia)
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García Moreno, Miguel Angel, primary, De la Garza Ramos, Myriam Angélica, additional, Martínez Ávila, Guillermo Cristian Guadalupe, additional, Gutiérrez Diez, Adriana, additional, Ojeda Zacarías, Ma Del Carmen, additional, and Aguirre-Arzola, Victor Eustorgio, additional
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- 2017
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14. Prevalence ofHelicobacter pylori vacAGenotypes andcagAGene in Dental Plaque of Asymptomatic Mexican Children
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Mendoza-Cantú, Alejandra, primary, Urrutia-Baca, Víctor Hugo, additional, Urbina-Ríos, Cynthia Sofía, additional, De la Garza-Ramos, Myriam Angélica, additional, García-Martínez, Martha Elena, additional, and Torre-Martínez, Hilda H. H., additional
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- 2017
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15. Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection.
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Espinosa-Ramos, David, Caballero-Hernández, Diana, Gomez-Flores, Ricardo, Trejo-Chávez, Armando, Pérez-Limón, Luis Jerónimo, de la Garza-Ramos, Myriam Angélica, Tamez-Guerra, Reyes, Tamez-Guerra, Patricia, and Rodriguez-Padilla, Cristina
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HELICOBACTER pylori ,IMMUNIZATION ,CONCANAVALIN A ,SIDE effects of antibiotics ,GASTRIC mucosa ,IMMUNOGLOBULIN M ,THYMIC stromal lymphopoietin - Abstract
Helicobacter pylori is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects. Furthermore, because there are no vaccines effective against H. pylori, an appropriate vaccine design targeting conserved/essential genes must be identified. In the present study, a H. pylori 50–52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against H. pylori infection. For this, mice received an intraperitoneal injection of 100 μg of H. pylori peptide on the first week, followed by two weekly subcutaneous reinforcements and further 10
9 bacteria administration in the drinking water for 3 weeks. Thymic cells proliferative responses to concanavalin A, serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokines, and IgG1, IgG2a, IgG2b, IgG3 IgM, and IgA immunoglobulins were evaluated. Significant (p<0.05) increases on lymphoproliferation and spleen weights after immunization were observed. In contrast, infection significantly (p<0.05) decreased lymphoproliferation, which was recovered in immunized mice. In addition, levels of serum TH1 and TH2 cytokines were not altered after immunization, except for the significant increase in IL-6 production in immunized and/or infected animals. Moreover, immunization correlated with plasma secretory IgA and IgG, whereas infection alone only elicited IgM antibodies. Peptide immunization protected 100% of mice against virulent H. pylori. MVTLINNE peptide deserves further research as an approach to the prophylaxis of H. pylori infection. [ABSTRACT FROM AUTHOR]- Published
- 2019
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16. Prevalence of Helicobacter pylori vacA Genotypes and cagA Gene in Dental Plaque of Asymptomatic Mexican Children.
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Mendoza-Cantú, Alejandra, Urrutia-Baca, Víctor Hugo, Urbina-Ríos, Cynthia Sofía, De la Garza-Ramos, Myriam Angélica, García-Martínez, Martha Elena, and Torre-Martínez, Hilda H. H.
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RISK factors of periodontal disease ,DENTAL plaque ,GASTROINTESTINAL diseases ,GINGIVITIS ,GINGIVA ,HELICOBACTER pylori ,PROBABILITY theory ,DISEASE prevalence ,GENOTYPES ,CHILDREN - Abstract
The variability in Helicobacter pylori vacA and cagA genes has been related to the progression of the gastrointestinal disease; also the presence of H. pylori in the oral cavity has been associated with periodontal disease in adults, but, in children without dyspeptic symptoms, little is known about this. We evaluated the prevalence of H. pylori and the presence of vacA/cagA genotypes in the oral cavity of Mexican children without dyspeptic symptoms. The gingival status was measured, and dental plaque samples (n=100) were taken. 38% of children were positive for H. pylori 16S rRNA gene by qPCR. A significant association between H. pylori oral infection and gingival status was observed (P<0.001). In 34.6% (9/26) of mild gingivitis cases, s1m2 genotype was found, while s1m1 was typed in 50% (3/6) of moderate gingivitis. The cagA prevalence among H. pylori-positive children was 80.8% (21/26), 83.3% (5/6), and 16.7% (1/6) of cases of mild gingivitis, moderate gingivitis, and nongingivitis, respectively (P<0.001). The s1m1/cagA+ combinational genotype was the most detected in children with gingivitis. Our results suggest that the prevalence of H. pylori and detection of vacA/cagA genotypes-associated gastrointestinal disease in the oral cavity could be related to the progression of gingivitis in asymptomatic children. [ABSTRACT FROM AUTHOR]
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- 2017
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17. A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of α-Solanine on Mouse Bone Marrow Mesenchymal Stem Cells.
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Ordóñez-Vásquez, Adriana, Jaramillo-Gómez, Lorenza, Duran-Correa, Camilo, Escamilla-García, Erandi, De la Garza-Ramos, Myriam Angélica, and Suárez-Obando, Fernando
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Αlpha-solanine (α-solanine) is a glycoalkaloid present in potato (Solanum tuberosum). It has been of particular interest because of its toxicity and potential teratogenic effects that include abnormalities of the central nervous system, such as exencephaly, encephalocele, and anophthalmia. Various types of cell culture have been used as experimental models to determine the effect of α-solanine on cell physiology. The morphological changes in the mesenchymal stem cell upon exposure to α-solanine have not been established. This study aimed to describe a reliable and reproducible model for assessing the structural changes induced by exposure of mouse bone marrow mesenchymal stem cells (MSCs) to different concentrations of α-solanine for 24 h. The results demonstrate that nonlethal concentrations of α-solanine (2–6 μM) changed the morphology of the cells, including an increase in the number of nucleoli, suggesting elevated protein synthesis, and the formation of spicules. In addition, treatment with α-solanine reduced the number of adherent cells and the formation of colonies in culture. Immunophenotypic characterization and staining of MSCs are proposed as a reproducible method that allows description of cells exposed to the glycoalkaloid, α-solanine. [ABSTRACT FROM AUTHOR]
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- 2017
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18. Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
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Del Angel-Mosqueda, Casiano, primary, Gutiérrez-Puente, Yolanda, additional, López-Lozano, Ada Pricila, additional, Romero-Zavaleta, Ricardo Emmanuel, additional, Mendiola-Jiménez, Andrés, additional, Medina-De la Garza, Carlos Eduardo, additional, Márquez-M, Marcela, additional, and De la Garza-Ramos, Myriam Angélica, additional
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- 2015
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19. Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro.
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Angel-Mosqueda, Casiano Del, Gutiérrez-Puente, Yolanda, López-Lozano, Ada Pricila, Romero-Zavaleta, Ricardo Emmanuel, Mendiola-Jiménez, Andrés, Medina-De la Garza, Carlos Eduardo, Márquez-M, Marcela, and De la Garza-Ramos, Myriam Angélica
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EPIDERMAL growth factor ,DENTAL pulp ,STEM cells ,CELL differentiation ,FIBROBLAST growth factors ,EXTRACELLULAR matrix - Abstract
Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs). Material and methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR. Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect. Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology. [ABSTRACT FROM AUTHOR]
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- 2015
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20. Análisis del efecto de la α-Solanina en las características citomorfológicas de células vivas adheridas y su relación con la expresión del gen Shroom3
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Ordóñez Vásquez, Adriana, Zarante Montoya, Ignacio Manuel, De La Garza-Ramos, Myriam Angélica, Garavito Aguilar, Zayra Viviana, Ortiz López, Rocio, Gutiérrez Gómez, María Lucía, Pérez Cardona, Olga Yanet, and Lattig Matiz, María Claudia
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Solanine ,Morphology ,Shroom ,Chinese ink ,Mesenchymal ,Solanina ,Doctorado en ciencias biológicas - Tesis y disertaciones académicas ,Morfología ,Morfología de las células ,Tinta china ,Célula ,Mesenquimal ,Expresión génica - Abstract
Dado el actual interés de diferentes investigadores hacia la α-solanina como potencial sustancia anticancerígena y la controversia que existe en la literatura científica sobre la posible citotoxicidad de la α-Solanina, así como las alteraciones que la sustancia provoca sobre diferentes células, se consideró de gran interés analizar los efectos que el glicoalcaloide produce en Células Troncales de Medula Ósea (CTMO). Con este fin se propuso la siguiente hipótesis de investigación; La observación y análisis de los cambios morfológicos resultado de la exposición de las CTMO a diferentes concentraciones y tiempos, permitirá proponer un modelo reproducible y unos patrones de comparación cualitativa que permitan analizar cambios de citotoxicidad básica mediante microscopia óptica en células vivas en cultivo. Este trabajo se realizó cumpliendo tres niveles de resolución: el primero, resolución macro, que corresponde a presentar la importancia de la α-solanina para la salud humana y expone la importancia de su análisis (Artículo publicado). En segundo lugar, resuelve dos problemas con los que nos enfrentamos al examinar el efecto morfológico que ocasiona el glicoalcaloide en este tipo de células mesenquimales inmunotipificadas, vivas y adheridas. Por un lado, no estaba descrita una técnica colorimétrica sencilla que permitiera visualizar en células vivas adheridas el efecto producido por el glicoalcaloide y por otro la ausencia en la literatura revisada, de la descripción de los rangos no letales, de concentración y tiempo de exposición al glicoalcaloide. Por estas razones se propuso validar la utilización de la tinta china como alternativa directa, nítida, económica y viable en el análisis del efecto ocasionado por la exposición de las células troncales de medula ósea de rata (CTMO) a dos concentraciones del glicoalcaloide, contenidas entre los rangos de experimentación resultado del análisis de referencias bibliográficas. (Artículo publicado). Por otro lado, no había evidencia en la literatura de las concentraciones del glicoalcaloide que permitieran evidenciar en este tipo de células mesenquimales, cambios morfológicos básicos ; 1. Cambios de forma de las células (Homogeneidad y Heterogeneidad celular predominante), 2. Cambios en tamaño y condensación intracitoplasmática, 3. Visualización o no de la membrana celular, 4. Presencia de prolongaciones o espículas modificadas en número y longitud, 5. Características de Núcleos, 6. Número de Nucléolos y 7. Presencia o no de vacuolas intracitoplasmáticas. (Artículo Publicado). Los cambios morfológicos evidenciados con la técnica propuesta y a las concentraciones sugeridas, fueron entonces validados en células vivas adheridas mediante la observación de la proteína globular actina, por medio de microscopia confocal y en células no adheridas, por medio del análisis de viabilidad (MTS-utilizando espectrofotometría), proliferación (CFSE) y apoptosis (Anexina V/7AAD) utilizando Citometría de Flujo. Los resultados demostraron que la α- Solanina produce cambios morfológicos visibles en microscopia convencional mayormente en las células tratadas durante 24 h con α- Solanina a una concentración de 2 μM, modificando parámetros relacionados con citotoxicidad basal, como forma, tamaño y longitud de espículas (Artículo Sometido) , así como también produciendo efectos característicos en núcleo, efectos característicos en la distribución de los microfilamentos de actina e inhibiendo la proliferación celular, promoviendo la apoptosis, y la autofagia (Artículo en revisión). Para el tercer nivel de resolución, se propuso el análisis de la posible relación entre el glicoalcaloide α-solanina y la expresión del gen Shroom3 descrito como regulador de la morfología celular en más de veinte tipos celulares incluidas las mesenquimales. Para esto, se expusieron células troncales de medula ósea de rata a dos concentraciones diferentes del glicoalcaloide y se realizó extracción de ARN y reacción cuantitativa en cadena de la polimerasa en tiempo real (qRT-PCR). Los resultados obtenidos en esta parte del trabajo proponen un vínculo entre el gen Shroom3 y la exposición a la α-Solanina, debido a que se encontró que el glicoalcaloide regula negativamente la expresión del gen de una manera dependiente de la concentración y del tiempo. En todos los niveles de resolución del trabajo se utilizaron células troncales de medula ósea de rata hembra cepa Lewis entre 2 y 4 meses de edad de la cepa Lewis alojadas en la Sala de Experimentación Animal de la Facultad de Odontología de Pontificia Universidad Javeriana. Estas células fueron obtenidas, extraídas, inmunotipificadas, caracterizadas y cultivadas en el Centro de Investigaciones Odontológicas bajo sus propios protocolos (en proceso de publicación según sus investigadores Jaramillo L., Duran C.). La α-solanina fue obtenida comercialmente. Pontificia Universidad Javeriana Given the current interest of different researchers towards α-solanine as a potential anti-cancer substance and the controversy that exists in the scientific literature about the possible cytotoxicity of α-Solanine, as well as the alterations that the substance causes on different cells, it was considered great interest to analyze the effects that the glycoalkaloid produces in Bone Marrow Truncal Cells (CTMO). To this end, the following research hypothesis was proposed; The observation and analysis of the morphological changes resulting from the exposure of the CTMO at different concentrations and times, will allow to propose a reproducible model and qualitative comparison patterns that allow to analyze changes of basic cytotoxicity by optical microscopy in living cells in culture. This work consider three levels of resolution: the first, macro resolution, which corresponds to presenting the importance of α-solanine for human health and exposes the importance of its analysis (Article published).Secondly, it solves two problems that we face when we examine the morphological effect caused by the glycoalkaloid in this type of immunotyped, alive and adherent mesenchymal cells. On the one hand, a simple colorimetric technique was not described that allowed to visualize in live adhered cells the effect produced by the glycoalkaloid and on the other hand the absence in the reviewed literature, of the description of the non-lethal ranges, of concentration and time of exposure to the glycoalkaloid. For these reasons it was proposed to validate the use of Chinese ink as a direct, clear, economical and viable alternative in the analysis of the effect caused by the exposure of rat bone marrow stem cells (CTMO) to two concentrations of glycoalkaloid, contained between the ranges of experimentation resulting from the analysis of bibliographic references. (Article published). On the other hand, there was no evidence in the literature of the concentrations of the glycoalkaloid that allowed to show in this type of mesenchymal cells, basic morphological changes; We observe 1. Changes in the shape of the cells (Homogeneity and predominant cellular heterogeneity), 2. Changes in size and intracytoplasmic condensation, 3. Visualization or not of the cell membrane, 4. Presence of prolongations or spicules modified in number and length, 5. Characteristics of Nuclei, 6. Number of Nucleoli and 7. Presence or not of intracytoplasmic vacuoles. (Article Published). The morphological changes evidenced with the proposed technique and the suggested concentrations were then validated in living adhered cells by observing the globular actin protein, by means of confocal microscopy and in non-adherent cells, by means of viability analysis (MTS-using spectrophotometry), proliferation (CFSE) and apoptosis (Annexin V / 7AAD) using Flow Cytometry. The results showed that α-Solanine produces visible morphological changes in conventional microscopy. Mainly in cells treated for 24 h with α-Solanine at a concentration of 2 μM, modifying parameters related to basal cytotoxicity, such as shape, size and length of spicules ( Submitted Article), as well as producing characteristic effects in nucleus, characteristic effects in the distribution of actin microfilaments and inhibiting cell proliferation, promoting apoptosis, and autophagy (Article under review). For the third level of resolution, we proposed the analysis of the possible relationship between the glycoalkaloid α-solanine and the expression of the Shroom3 gene described as a regulator of cell morphology in more than twenty cell types including mesenchymal. For this, we expose rat bone marrow to two different glycoalkaloid concentrations and we do RNA (qRT-PCR). The results obtained in this part of the work propose a link between the Shroom3 gene and exposure to α-Solanine, because found that the glycoalkaloid negatively regulates the expression of the gene in a manner dependent on concentration and time. At all levels of resolution we used stem cells of bone rat marrow female Lewis strain between 2 and 4 months of age of the Lewis strain housed in the Animal Experimentation Room of the Faculty of Dentistry of Pontificia Universidad Javeriana. These cells were obtained, extracted, immunotyped, characterized and cultured in the Dental Research Center under their own protocols (in publication process according to their researchers Jaramillo L., Duran C.). α-Solanine was obtained commercially. Doctor en Ciencias Biológicas Doctorado
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- 2019
21. Application of Robotics in Orthodontics: A Systematic Review.
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De La Garza-Ramos MA, Ipiña-Lozano HH, Cano-Verdugo G, Nakagoshi-Cepeda MAA, and Liu Y
- Abstract
Robotics has various applications in dentistry, particularly in orthodontics, although the potential use of these technologies is not yet clear. This review aims to summarize the application of robotics in orthodontics and clarify its function and scope in clinical practice. Original articles addressing the application of robotics in any area of orthodontic practice were included, and review articles were excluded. PubMed, Google Scholar, Scopus, and DOAJ were searched from June to August 2023. The risk of bias was established using the risk of bias in non-randomized studies (ROBINS) and certainty assessment tools following the grading of recommendations, assessment, development, and evaluation (GRADE) guidelines. A narrative synthesis of the data was generated and presented according to its application in surgical and non-surgical orthodontics. The search retrieved 2,106 articles, of which 16 articles were selected for final data synthesis of research conducted between 2011 and 2023 in Asia, Europe, and North America. The application of robotics in surgical orthodontics helps guide orthognathic surgeries by reducing the margin of error, but it does not replace the work of a clinician. In non-surgical orthodontics, robotics assists in performing customized bending of orthodontic wires and simulating orthodontic movements, but its application is expensive. The articles collected for this synthesis exhibited a low risk of bias and high certainty, and the results indicated that the advantages of the application of robotics in orthodontics outweigh the disadvantages. This project was self-financed, and a previous protocol was registered at the PROSPERO site (registration number: CRD42023463531)., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, De La Garza-Ramos et al.)
- Published
- 2024
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