Search

Mycotoxin contamination in grain has been an ongoing concern in the world. Wheat, as a staple crop in China, is particularly notable for its mycotoxin contamination. The main mycotoxins in wheat include deoxynivalenol (DON) and its derivates, zearalenone (ZEN) and aflatoxin B1 (AFB1). After harvest, drying process is an effective technique and a necessary step to ensure the long-term safe storage of wheat. In this study, the moisture content, the concentrations of total fungi and main mycotoxins in post-harvest wheat of three wheat growing areas in the North China Plain were examined, and the effect of different drying methods on wheat quality was evaluated. The results showed that 87.5% of wheat samples were simultaneously contaminated with two or more mycotoxins. Due to the pre-harvest heavy rainfall, the moisture content, the levels of total fungi and mycotoxins in wheat samples of Liaocheng city were significantly higher compared to other regions. Moreover, the effects of different drying methods on the starch gelatinization and viscosity properties of wheat were investigated. The results showed that both natural air drying and dryer drying altered the crystal structure within starch particles and affected the gelatinization and viscosity properties of wheat starch. However, there is no significant difference between the wheat samples treated with two drying methods.

Abstract Background Long-term monitoring of Electrocardiogram (ECG) recordings is crucial to diagnose arrhythmias. Clinicians can find it challenging to diagnose arrhythmias, and this is a particular issue in more remote and underdeveloped areas. The development of digital ECG and AI methods could assist clinicians who need to diagnose arrhythmias outside of the hospital setting. Methods We constructed a large-scale Chinese ECG benchmark dataset using data from 272,753 patients collected from January 2017 to December 2021. The dataset contains ECG recordings from all common arrhythmias present in the Chinese population. Several experienced cardiologists from Shanghai First People’s Hospital labeled the dataset. We then developed a deep learning-based multi-label interpretable diagnostic model from the ECG recordings. We utilized Accuracy, F1 score and AUC-ROC to compare the performance of our model with that of the cardiologists, as well as with six comparison models, using testing and hidden data sets. Results The results show that our approach achieves an F1 score of 83.51%, an average AUC ROC score of 0.977, and 93.74% mean accuracy for 6 common arrhythmias. Results from the hidden dataset demonstrate the performance of our approach exceeds that of cardiologists. Our approach also highlights the diagnostic process. Conclusions Our diagnosis system has superior diagnostic performance over that of clinicians. It also has the potential to help clinicians rapidly identify abnormal regions on ECG recordings, thus improving efficiency and accuracy of clinical ECG diagnosis in China. This approach could therefore potentially improve the productivity of out-of-hospital ECG diagnosis and provides a promising prospect for telemedicine.

Due to the Gibbs oscillation effect of microwave aperture synthesis radiometers (ASRs) occurring near land/ocean transitions, soil moisture and ocean salinity (SMOS) brightness temperature (TB) measurements are subject to land contamination in ocean regions (within a few hundred kilometers of the shore). In this article, the magnitude of land contamination in the SMOS L1C TB is demonstrated by comparison with the results of collocated forward modeling. Then, a land contamination correction algorithm for microwave SARs based on a convolutional neural network is presented. The basic process of the algorithm is to design a land contamination mitigation network (LCMN) to learn the land contamination features from the simulated TB dataset of the microwave ASRs and then to use the well-trained network to remap the actual observed TB images to mitigate the land contamination error. Land contamination error correction experiments based on LCMN remapping were executed on SMOS L1C TB data, and the performance of the algorithm was verified by comparison with forward modeling and salinity retrieval. The experimental results show that the proposed LCMN algorithm can effectively mitigate land contamination at the SMOS L1C TB.

In practical industrial environment, variable working condition can result in shifts in data distributions, and the labeled fault data in various working conditions is difficult to collect because rotating machines often works in normal status, and the insufficient labeled fault data brings data samples imbalance and performance degradation of intelligent fault diagnosis model. To overcome these problems, by integrating the superiority of deep learning method and feature-based transfer learning method, this work proposes an innovative cross-domain fault diagnosis framework based on deep transfer convolutional neural network and supervised joint matching. First, the continue wavelet transform is used to process original bearing vibration signals and extract time-frequency images. Second, a deep transfer convolutional neural network is built by the way of fine-tuning, and the trained network is used to extract deep features from different domains. Third, a new domain adaptation approach, supervised joint matching, is developed to conduct joint feature distribution matching and instance reweighting with the consideration of maximum marginal criterion. The intelligent bearing fault diagnosis model is then trained to predict the labels of the target domain's feature data. To verify the performance of the proposed approaches, this study uses two distinct datasets pertaining to bearing defects for conducting cross-domain fault diagnosis in the presence of balanced and imbalanced data. The experimental analysis indicates that the designed methods can achieve desirable diagnostic accuracy and possess robust generalization ability.

Most W mineralization in the world is genetically related with highly fractionated granites, but little is known about ore-forming fluid evolution of W mineralization associated with weakly fractionated granites. To reveal the ore-forming fluid evolution of W mineralization related to the weakly fractionated granites, a combined study of field and petrographic observations, mineralogical, morphological, and in-situ geochemical data of different-type quartz from Dongyuan porphyry W deposit in the world-class Jiangnan W belt, China, was carried out. The petrographic observation and cathodoluminescence (CL) imaging revealed the quartz in the Dongyuan W deposit can be divided into hydrothermal quartz (Qz1 with core-edge structure, and Qz2 with oscillating zone) in the mineralized alteration zone, and magmatic quartz (Qz3 with inherited core) in granodiorite porphyry. The LA-ICP-MS results of the Dongyuan quartz samples show that Al may enter the quartz structure with trivalent Al3+ instead of Si4+, monovalent alkali metals and bivalent (Ge2+, Sr2+) cations is mainly of charge compensators in quartz, while Ti possibly is in the form of microinclusions of titanium-containing minerals in quartz. The integrated interpretation of the trace element compositions (e.g., Al and Ti contents, Al/Na ratios) and CL texture of quartz indicate high temperature (>400 °C) and uniform Al-rich acidic fluid are conducive to W precipitation of the Dongyuan deposit. Furthermore, trace element compositions (e.g., Ge, Al) and micro-textures of quartz have the potential for distinguishing magmatic and hydrothermal quartz in the Dongyuan W deposit.

High-entropy alloys (HEAs) have garnered significant interest in recent years due to their exceptional properties. Among the HEAs, the AlCoCrFeNi HEA has been extensively studied due to its outstanding thermal stability and mechanical properties. However, limited attention has been given to its tribological behavior under high temperature and high velocity conditions. In this study, we first investigate the effect of temperature, scratching velocity, and scratching depth on the tribological behavior of AlCoCrFeNi HEA at the atomic level employing molecular dynamics method. The AlCoCrFeNi HEA exhibits good wear resistance at high temperature due to fewer dislocations to nucleation and slip. Regardless of a significant increase in scratching velocity, there is little difference in the wear volume, indicating that the AlCoCrFeNi HEA still maintains excellent wear resistance even under extremely high scratching velocity. The stacking fault tetrahedron is generated in the AlCoCrFeNi HEA under the large scratching depths of 1.5 nm and 2.0 nm, implying that the wear resistance of AlCoCrFeNi HEA would be improved after the nanoscratching with a relatively large scratching depth. These findings are highly significant for expanding the application potential of AlCoCrFeNi HEA under extreme conditions and provide profound insights into the tribological behavior of the HEA.

10-hydroxy-2-decenoic acid (10-HDA), which is a unique bioactive fatty acid of royal jelly synthesized by nurse bees for larvae and adult queen bees, is recognized for its dual utility in medicinal and nutritional applications. Previous research has indicated that 10-HDA exerts antitumor effects on numerous tumor cell lines, including colon cancer cells, A549 human lung cancer cells, and human hepatoma cells. The present study extends this inquiry to lymphoma, specifically evaluating the impact of 10-HDA on the SU-DHL-2 cell line. Our findings revealed dose-dependent suppression of SU-DHL-2 cell survival, with an IC50 of 496.8 μg/mL at a density of 3 × 106 cells/well after 24 h. For normal liver LO2 cells and human fibroblasts (HSFs), the IC50 values were approximately 1000 μg/mL and over 1000 μg/mL, respectively. The results of label-free proteomics revealed 147 upregulated and 347 downregulated differentially expressed proteins that were significantly enriched in the complement and coagulation cascades pathway (adjusted p-value = 0.012), including the differentially expressed proteins prothrombin, plasminogen, plasminogen, carboxypeptidase B2, fibrinogen beta chain, fibrinogen gamma chain, and coagulation factor V. The top three hub proteins, ribosomal protein L5, tumor protein p53, and ribosomal protein L24, were identified via protein–protein interaction (PPI) analysis. This result showed that the complement and coagulation cascade pathways might play a key role in the antitumor process of 10-HDA, suggesting a potential therapeutic avenue for lymphoma treatment. However, the specificity of the effect of 10-HDA on SU-DHL-2 cells warrants further investigation.

Abstract Background Cancer patients can achieve dramatic responses to chemotherapy yet retain resistant tumor cells, which ultimately results in relapse. Although xenograft model studies have identified several cellular and molecular features that are associated with chemoresistance in acute myeloid leukemia (AML), to what extent AML patients exhibit these properties remains largely unknown. Results We apply single-cell RNA sequencing to paired pre- and post-chemotherapy whole bone marrow samples obtained from 13 pediatric AML patients who had achieved disease remission, and distinguish AML clusters from normal cells based on their unique transcriptomic profiles. Approximately 50% of leukemic stem and progenitor populations actively express leukemia stem cell (LSC) and oxidative phosphorylation (OXPHOS) signatures, respectively. These clusters have a higher chance of tolerating therapy and exhibit an enhanced metabolic program in response to treatment. Interestingly, the transmembrane receptor CD69 is highly expressed in chemoresistant hematopoietic stem cell (HSC)-like populations (named the CD69 + HSC-like subpopulation). Furthermore, overexpression of CD69 results in suppression of the mTOR signaling pathway and promotion of cell quiescence and adhesion in vitro. Finally, the presence of CD69 + HSC-like cells is associated with unfavorable genetic mutations, the persistence of residual tumor cells in chemotherapy, and poor outcomes in independent pediatric and adult public AML cohorts. Conclusions Our analysis reveals leukemia stem cell and OXPHOS as two major chemoresistant features in human AML patients. CD69 may serve as a potential biomarker in defining a subpopulation of chemoresistant leukemia stem cells. These findings have important implications for targeting residual chemo-surviving AML cells.

Abstract One of the characteristics of leukemia is that it contains multiple rearrangements of signal transduction genes and overexpression of non-mutant genes, such as transcription factors. As an important regulator of hematopoietic stem cell development and erythropoiesis, LMO2 is considered an effective carcinogenic driver in T cell lines and a marker of poor prognosis in patients with AML with normal karyotype. LDB1 is a key factor in the transformation of thymocytes into T-ALL induced by LMO2, and enhances the stability of carcinogenic related proteins in leukemia. However, the function and mechanism of LMO2 and LDB1 in AML remains unclear. Herein, the LMO2 gene was knocked down to observe its effects on proliferation, survival, and colony formation of NB4, Kasumi-1 and K562 cell lines. Using mass spectrometry and IP experiments, our results showed the presence of LMO2/LDB1 protein complex in AML cell lines, which is consistent with previous studies. Furthermore, in vitro and in vivo experiments revealed that LDB1 is essential for the proliferation and survival of AML cell lines. Analysis of RNA-seq and ChIP-Seq results showed that LDB1 could regulate apoptosis-related genes, including LMO2. In LDB1-deficient AML cell lines, the overexpression of LMO2 partially compensates for the proliferation inhibition. In summary, our findings revealed that LDB1 played an important role in AML as an oncogene, and emphasize the potential importance of the LMO2/LDB1 complex in clinical treatment of patients with AML.

African swine fever (ASF) is a viral disease caused by the African swine fever virus that can be highly transmitted and lethal in domestic pigs. In the absence of a vaccine, effective diagnosis is critical for minimizing the virus’s spread. In recent years, with the decline of African swine fever virus (ASFV) virulence, antibody detection has become an important means of detection. ASFV nucleocapsid protein p34 is a mature hydrolytic product of pp220, which is highly conserved and has a high content in the structural protein of the virus. Prokaryotic cells were chosen to generate highly active and high-yield p34 protein, which was then used as an antigen for producing mouse monoclonal antibodies. The B-cell epitope 202QKELDKLQT210, which was highly conserved and found on the surface of the p34 protein, was first identified by an anti-p34 monoclonal antibody utilizing the peptide scanning technique and visualized in helix. This supported the viability of p34 protein detection even further. In addition, we established an indirect ELISA assay based on p34 to detect ASFV antibodies. The coincidence rate of this method with commercially available kits was shown to be 97.83%. Sensitivity analysis revealed that it could be detected in serum dilution as low as 1:6400, and there was no cross-reaction with other prevalent porcine epidemic diseases classical swine fever virus (CSFV), foot-and-mouth disease virus (FMDV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus 2 (PCV2). In summary, the established ELISA method and anti-P34 monoclonal antibody have demonstrated that the p34 protein has a promising application prospect for the detection of African swine fever antibodies.

ABSTRACTIntroduction B-cell acute lymphoblastic leukemia (B-ALL) is the most prevalent malignant tumor affecting children. While the majority of B-ALL patients (90%) experience successful recovery, early relapse cases of B-ALL continue to exhibit high mortality rates. MZ1, a novel inhibitor of Bromodomains and extra-terminal (BET) proteins, has demonstrated potent antitumor activity against hematological malignancies. The objective of this study was to examine the role and therapeutic potential of MZ1 in the treatment of B-ALL.Methods In order to ascertain the fundamental mechanism of MZ1, a sequence of in vitro assays was conducted on B-ALL cell lines, encompassing Cell Counting Kit 8 (CCK8) assay, Propidium iodide (PI) staining, and Annexin V/PI staining. Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) were employed to examine protein and mRNA expression levels. Transcriptomic RNA sequencing (RNA-seq) was utilized to screen the target genes of MZ1, and lentiviral transfection was employed to establish stably-expressing/knockdown cell lines.Results MZ1 has been observed to induce the degradation of Bromodomain Containing 4 (BRD4), Bromodomain Containing 3 (BRD3), and Bromodomain Containing 2 (BRD2) in B-ALL cell strains, leading to inhibited cell growth and induction of cell apoptosis and cycle arrest in vitro. These findings suggest that MZ1 exhibits cytotoxic effects on two distinct molecular subtypes of B-ALL, namely 697 (TCF3/PBX1) and RS4;11 (MLL-AF4) B-ALL cell lines. Additionally, RNA-sequencing analysis revealed that MZ1 significantly downregulated the expression of Cyclin D3 (CCND3) gene in B-ALL cell lines, which in turn promoted cell apoptosis, blocked cell cycle, and caused cell proliferation inhibition.Conclusion Our results suggest that MZ1 has potential anti-B-ALL effects and might be a novel therapeutic target.

The key to whether elderly individuals in the community can enjoy their later years peacefully lies in the service capabilities of community comprehensive elderly care service facilities (CCECSF) under the Public-Private Partnership (PPP) mode. To maintain a high level of service capability in community comprehensive elderly care service facilities under PPP mode, scientific evaluation of the effectiveness of these facilities is equally crucial. This article first constructs a post-occupancy evaluation index system of community comprehensive elderly care service facilities under PPP mode that includes three evaluation attributes and fifteen evaluation criteria based on the Chinese culture and lifestyle habits. Regarding the issue of direct users being unable to directly participate in evaluations, the uncertainty in evaluation information, and the volatility of evaluation results, a multi-stage post-occupancy evaluation model is constructed based on probabilistic linguistic term set, TOPSIS model and multi-stage decision theory. The above post-occupancy evaluation index system and evaluation model together constitute a multi-stage post-occupancy evaluation framework for community comprehensive elderly care service facilities under PPP mode. The outcomes of the case study indicate that the post-occupancy evaluation index system can offer a scientifically guided approach for evaluating the service level of community comprehensive elderly care service facilities under the PPP mode; meanwhile, the multi-stage evaluation model can enable direct user participation in the post-evaluation of facility usage and improve the robustness and reduce the fluctuation of the evaluation results, so as to improve the scientificity of the evaluation results.

Vasculogenic mimicry (VM) differs from the classical tumor angiogenesis model. VM does not depend on endothelial cells; instead, highly aggressive tumor cells mimic endothelial cells to form a vascular-like channel structure. VM mediated by tumor cells is significantly and positively associated with a poor prognosis and low survival rates in patients with highly aggressive cancer. In the treatment of highly aggressive malignancies, the presence of VM is considered an important reason for the unsatisfactory clinical efficacy of anti-tumor–angiogenesis therapy (e.g., therapy targeting vascular endothelial growth factor A). Many targeted therapeutic drugs based on traditional tumor blood vessels have been used clinically. Although some progress has been made in certain tumors, problems such as drug resistance have restricted the expected therapeutic effects. Thrombospondin 2 (THBS2) is one of the most important genes associated with angiogenesis, and this gene exerts angiogenesis-related functions through the PI3K/AKT signaling pathway. Although the PI3K/AKT/mTOR signaling pathway is closely related to the progression of VM, the mechanism by which the promising biomarker THBS2 participates in and regulates tumor VM by activating the PI3K/AKT/mTOR signaling pathway is unclear. In this review, we analyze the monomer structure and biological activity of THBS2, the structure and potential synthesis mechanisms of VM, and the complex mechanisms between THBS2, the PI3K/AKT/mTOR signaling pathway, and VM.

BackgroundMethotrexate (MTX), utilized as a graft-versus-host disease (GvHD) prophylactic agent in allogeneic hematopoietic stem cell transplantation (allo-HSCT), has been proven to effectively decrease the occurrence of the peri-engraftment syndrome (Peri-ES) and acute GvHD (aGvHD). Changes in the pharmacodynamics of MTX are closely associated with gene polymorphisms in genes encoding drug-metabolizing enzymes and transporters. Nevertheless, the current studies mainly concentrate on leukemia or autoimmune diseases, and limited studies on allo-HSCT were reported.MethodsHere, we retrospectively assessed the relationship between MTX-related transporter and metabolizing enzyme gene polymorphisms, clinical characteristics, and outcomes in 57 pediatric patients who received haploid HSCT (haplo-HSCT) with malignant tumors at a single center.ResultsWe discovered all gene polymorphisms were in the Hardy–Weinberg equilibrium in our cohort. We discovered a significant correlation between platelet recovery time and ABCB1 (1236C>T) (p = 0.042). Compared with patients with SLCO1B1 (1865+4846T>C) TT, patients with SLCO1B1 (1865+4846T>C) TC/CC had an increased incidence of Peri-ES (p = 0.030). Based on the multivariate Cox analysis, we discovered that SLCO1B1 (1865+4846T>C) TT genotype was an independent protective factor for Peri-ES morbidity (hazard ratio (HR) = 0.464, p = 0.031), and the dose of mononuclear cells reinfused was significantly correlated with II–IV aGvHD (HR = 2.604, p = 0.039).ConclusionIn summary, our findings prove that the host’s genotypes might modify the risk of developing Peri-ES, contribute to a better understanding of the inter-individual difference in efficacy, and facilitate the development of individualized approaches to GvHD prophylaxis.

Micropterus salmoides is an economically important cultured carnivorous fish in China. In recent years, owing to the development and wide application of artificial formula feed, M. salmoides production has rapidly increased and reached 470 000 tons in 2019. However, a low survival rate of M. salmoides larvae is observed during the first feeding and artificial formula feed adaptation.In this study, to better understand the artificial formula feed adaptation of M. salmoides larvae, the developmental characteristics of the digestive tract and digestive gland of the fish larvae from 2~30 dph were observed and described using histological sections and scanning electron microscopy (SEM). Moreover, the digestive tract (stomach and intestine) characteristics of certain dead larval fish during the first feeding and the transformation of artificial formula feed were investigated.For histological analysis, the larval fish (including stomach, intestine, pyloric cecum, liver, and pancreas tissues) were dehydrated with an alcohol gradient (70%, 80%, 90%, and 100%), embedded in paraffin, cut into 5 μm sections, and stained with standard hematoxylin and eosin.For SEM analysis, the stomach, pyloric cecum, and intestine of 30 dph larval fish were fixed in 2.5% glutaraldehyde solution for 12 h. Then, the tissues were fixed in 1% osmium solution for 2 h, dehydrated with gradient alcohol (70%~100%), soaked in tert-butyl alcohol for 2 h, dried with a lyophilizer, and plated with gold by ion sputtering. Finally, the images were captured using a HITACHIX-650 scanning electron microscope.At a water temperature of (23±1)℃, the larval yolk sac and oil drop gradually decreased at 0~4 dph, the digestive tract was initially differentiated, and heartbeat, blood circulation, mouth crack, esophagus, and anus were observed at 4 dph. Larval fish were in the endogenous nutrition period at this stage. In 4~ 6 dph larval fish, the esophagus, stomach, intestine, liver, and pancreas regions gradually formed, the digestive tube opened to the outside initially, and yolk sac and oil drop significantly decreased and then completely disappeared, indicating that the larval fish entered the endo-exotrophic period. At this stage, sufficient Artemia salina should be provided to induce the larval fish to open their mouth and perform first feeding. In 10~16 dph larval fish, the stomach, pylorus caecum, and intestine were closely arranged. From the surface of the intestinal cavity, the mucosa, submucosa, muscular, and serosa layers were successively presented. The size of the mucosa columnar epithelial cells and the number of goblet cells clearly increased. The masses of hepatocytes and pancreatic cells significantly increased, and a vacuole structure appeared in the hepatocytes. The pancreatic cells were arranged closely, and the blood cells and pancreatic ducts were distributed. At this stage, larval fish entered the exogenous nutrition period. From this stage onward, larval fish are better able to transform feeding artificial formula feed step by step. In 20~24 dph larval fish, the stomach gland was well developed, and the stomach wall thickness and number of stomach glands further increased. Furthermore, the mucosal folds and connective tissue of the submucosa were further differentiated, and the longitudinal mucosal folds increased and curled. In addition, secondary mucosal folds in the intestine gradually appeared and the number of mucosal folds in the pyloric cecum increased. Fatty accumulation and secretory granules were observed in the liver and pancreas, respectively, indicating that the larval hepatopancreas was similar to that of adult fish. The digestive system of larval fish was completely developed at this stage, and the larval fish had the ability to transform and adapt to artificial formula feed.No significant differences were observed in body length and body weight of M. salmoides larvae (P > 0.05) during the adaptation to artificial formula feed at 12, 16, and 20 dph. However, the survival rate of 12 dph larval fish (45.59%) was lower than that of 16 (60.60%) and 20 dph larval fish (69.83%). In addition, the body length, body weight, and survival rate of M. salmoides larvae were positively correlated with the time point of feeding artificial formula feed.SEM results showed abundant polygonal reticular mucosal folds in the gastric epidermis of 30-day-old larval fish, and there were dense secretion pores between the gastric pits. Mucosa folds with fixed shapes were observed on the inner surface of the intestine, and mucous cells, secretory pores, and secretory granules were clearly observed between the mucosal folds. Interestingly, the inner surface structure of the pyloric cecum was similar to that of the intestine. A difference was the mucosa folds without a fixed shape on the inner surface of the pyloric caecum. These mucosal folds and secretory pores are important for food digestion, absorption, and excretion, indicating that the larval fish are old enough to adapt to the artificial formula feed at this point.During the first feeding and artificial formula feed adaptation, the stomach and intestine of the larval fish were incompletely developed and accompanied by tissue damage in the dead individuals. For example, the stomach and intestinal cavity were significantly shriveled, the inner epidermis did not have the molding mucous membrane fold and goblet cells, and the mucous membrane layer was cracked or absent. These results indicate that larval fish overfeed and fail to effectively digest, absorb, and expel nutrients, which ultimately results in death associated with nutritional deficiencies, intestinal blockages, and inflammation.During the process of adaptation to artificial formula feed for M. salmoides, the development of larval digestive system was investigated. The digestive system of larval fish at 4~6 dph gradually differentiated and was still in the mixed nutrition period, and sufficient A. salina should be provided to induce the larval fish to open their mouths and perform the first feeding. At 6~16 dph, the digestive system gradually developed and larval fish entered the exogenous nutrition period, and sufficient food could be provided. At 16~20 dph, the digestive system of the larval fish completely developed, and this stage is the optimal time to switch to artificial formula feed. Our study provides basic data for feeding condition optimization of M. salmoides larvae.

Acute myeloid leukemia (AML) is a highly cancerous and aggressive hematologic disease with elevated levels of drug resistance and relapse resulting in high mortality. Recently, bromodomains and extra-terminal (BET) protein inhibitors have been extensively researched in hematological tumors as potential anticancer agents. MZ1 is a novel BET inhibitor that mediates selective proteins degradation and suppression of tumor growth through proteolysis-targeting chimeras (PROTAC) technology. Accordingly, this study aimed to investigate the role and therapeutic potential of MZ1 in AML. In this study, we first identified that AML patients with high BRD4 expression had poor overall survival than those with low expression group. MZ1 inhibited AML cell growth and induced apoptosis and cycle arrest in vitro. MZ1 induced degradation of BRD4, BRD3 and BRD2 in AML cell strains. Additionally, MZ1 also initiated the cleavage of poly-ADP-ribose polymerase (PARP), which showed cytotoxic effects on NB4 (PML-RARa), K562 (BCR-ABL), Kasumi-1 (AML1-ETO), and MV4-11 (MLL-AF4) cell lines representing different molecular subtypes of AML. In AML mouse leukemia model, MZ1 significantly decreased leukemia cell growth and increased the mouse survival time. According to the RNA-sequencing analysis, MZ1 led to c-Myc and ANP32B genes significant downregulation in AML cell lines. Knockdown of ANP32B promoted AML cell apoptosis and inhibited cell growth. Overall, our data indicated that MZ1 had broad anti-cancer effects on AML cell lines with different molecular lesions, which might be exploited as a novel therapeutic strategy for AML patients.

Background Hemorrhagic cystitis (HC) is a severe complication of allo-HSCT, characterized by irritative symptoms of the urinary tract and a higher morbidity rate. The risk factors and prognosis of HC are still unclear.Objective The objective of this study is to identify risk factors and outcomes to improve treatment in pediatric SAA patients undergoing HSCTs in the Children's Hospital of Soochow University.Methods A total of 97 SAA patients as a cohort were enrolled from 2010 to 2019 in the Children's Hospital of Soochow University and a number of factors related to HC and outcomes were analysed. In all transplants (except UCBT), patients received a combination of G-CSF stimulated bone marrow (BM) and peripheral blood stem cell (PBSC). The minimum number of CD34 + cells is 5 × 106 cells/kg.Results Mononuclear cells dose (MNC, cut off: 8.53 × 108/kg) and grade II–IV acute graft versus host disease (aGVHD) were identified as independent risk factors for HC. Patients without HC had better overall survival (OS) than with HC (No HC: 98.6%±1.4% vs HC: 87.4% ± 6.8%, p = 0.03).Conclusion We concluded that aGVHD and MNC dose in graft might play an important role in the development of HC in pediatric SAA patients undergoing allo-HSCT. HC is also a key complication affecting the prognosis of children with SAA after allo-HSCT.

Spike (S) glycoprotein is the most significant structural protein of SARS-CoV-2 and a key target for neutralizing antibodies. In light of the on-going SARS-CoV-2 pandemic, identification and screening of epitopes of spike glycoproteins will provide vital progress in the development of sensitive and specific diagnostic tools. In the present study, NTD, RBD, and S2 genes were inserted into the pcDNA3.1(+) vector and designed with N-terminal 6× His-tag for fusion expression in HEK293F cells by transient transfection. Six monoclonal antibodies (4G, 9E, 4B, 7D, 8F, and 3D) were prepared using the expressed proteins by cell fusion technique. The characterization of mAbs was performed by indirect -ELISA, western blot, and IFA. We designed 49 overlapping synthesized peptides that cover the extracellular region of S protein in which 6 amino acid residues were offset between adjacent (S1–S49). Peptides S12, S19, and S49 were identified as the immunodominant epitope regions by the mAbs. These regions were further truncated and the peptides S12.2 286TDAVDCALDPLS297, S19.2 464FERDISTEIYQA475, and S49.4 1202ELGKYEQYIKWP1213 were identified as B- cell linear epitopes for the first time. Alanine scans showed that the D467, I468, E471, Q474, and A475 of the epitope S19.2 and K1205, Q1208, and Y1209 of the epitope S49.4 were the core sites involved in the mAbs binding. The multiple sequence alignment analysis showed that these three epitopes were highly conserved among the variants of concern (VOCs) and variants of interest (VOIs). Taken together, the findings provide a potential material for rapid diagnosis methods of COVID-19.

Measurement(s) motion of construction workers Technology Type(s) wearable motion sensing system Factor Type(s) skeleton joints position Sample Characteristic - Organism construction workers Sample Characteristic - Environment construction site

Abstract Fabric‐based materials have demonstrated promise for high‐performance wearable applications but are currently restricted by their deficient mechanical properties. Here, this work leverages the design freedom offered by additive manufacturing and a novel interlocking pattern to for the first time fabricate a dual‐faced chain mail structure consisting of 3D re‐entrant unit cells. The flexible structured fabric demonstrates high specific energy absorption and specific strength of up to 1530 J kg−1 and 5900 Nm kg−1, respectively, together with an excellent recovery ratio of ≈80%, thereby overcoming the strength–recoverability trade‐off. The designed dual‐faced structured fabric compares favorably against a wide range of materials proposed for wearable applications, attributed to the synergetic strengthening of the energy‐absorbing re‐entrant unit cells and their unique topological interlocking. This work advocates the combined design of energy‐absorbing unit cells and their interlocking to extend the application prospects of fabric‐based materials to shape‐adaptive protection.

In recent years, vegetable production has been in the transitional stage from traditional cultivation mode to modern cultivation mode. Therefore, evaluating the actual productivity and economic benefits of different cultivation modes is instructive for leafy vegetable production. This research chose a vegetable production company (Shanghai, China) as a demonstration case to cultivate various leaf vegetables under different cultivation modes (hydroponic, substrate, and soil cultivation). By investigating the yield and quality of vegetables, as well as all production costs and selling prices, we drive a comparative evaluation of comprehensive features and economic benefits of different cultivation modes. The results showed that leafy vegetables cultivated by hydroponic mode gain the highest fresh weight at the early growth stage, but the greatest unit yield (0.98~1.21 kg·m−2) emerged under the soil cultivation mode. Vegetables grown under substrate mode had the best comprehensive quality with high soluble solids content and low nitrate content. The unit costs of the soilless cultivation modes (8.26~10.3 CNY·m−2) were 3–4-fold higher than that of soil cultivation, mainly due to the high fixed costs and input costs, especially for substrate mode. According to actual prices, the soil cultivation mode had the highest economic benefits as a whole (4.83~8.83 CNY·m−2), and only hydroponic cabbage which had comparable yield gained considerable benefits (8.94 CNY·m−2). Quality-based pricing scheme can make substrate mode achieve profitability. The pricing scheme that guarantees a 40% revenue is within acceptable limits for consumers. Based on this actual production case, it can be concluded that a low unit yield of hydroponic mode and a high unit cost of substrate mode are the predominant factors affecting their profitability and economic viability.

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a common food-borne pathogen that commonly causes gastroenteritis in humans and animals. Apis laboriosa honey (ALH) harvested in China has significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. We hypothesize that ALH has antibacterial activity against S. Typhimurium. The physicochemical parameters, minimum inhibitory and bactericidal concentrations (MIC and MBC) and the possible mechanism were determined. The results showed that there were significantly different physicochemical parameters, including 73 phenolic compounds, among ALH samples harvested at different times and from different regions. Their antioxidant activity was affected by their components, especially total phenol and flavonoid contents (TPC, TFC), which had a high correlation with antioxidant activities except for the O2- assay. The MIC and MBC of ALH against S. Typhimurium were 20–30% and 25–40%, respectively, which were close to those of UMF5+ manuka honey. The proteomic experiment revealed the possible antibacterial mechanism of ALH1 at IC50 (2.97%, w/v), whose antioxidant activity reduced the bacterial reduction reaction and energy supply, mainly by inhibiting the citrate cycle (TCA cycle), amino acid metabolism pathways and enhancing the glycolysis pathway. The results provide a theoretical basis for the development of bacteriostatic agents and application of ALH.

Propolis is a gelatinous substance processed by western worker bees from the resin of plant buds and mixed with the secretions of the maxillary glands and beeswax. Propolis has extensive biological activities and antitumor effects. There have been few reports about the antitumor effect of propolis against human cutaneous squamous cell carcinoma (CSCC) A431 cells and its potential mechanism. CCK-8 assays, label-free proteomics, RT–PCR, and a xenograft tumor model were employed to explore this possibility. The results showed that the inhibition rate of A431 cell proliferation by the ethanol extract of propolis (EEP) was dose-dependent, with an IC50 of 39.17 μg/mL. There were 193 differentially expressed proteins in the EEP group compared with the control group (p < 0.05), of which 103 proteins (53.37%) were upregulated, and 90 proteins (46.63%) were downregulated. The main three activated and suppressed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were extracellular matrix (ECM)-receptor interaction, amoebiasis, cell adhesion molecules (CAMs), nonalcoholic fatty liver disease (NAFLD), retrograde endocannabinoid signaling, and Alzheimer’s disease. The tumor volume of the 100 mg/kg EEP group was significantly different from that of the control group (p < 0.05). These results provide a theoretical basis for the potential treatment of human CSCC A431 cell tumors using propolis.

The global concern regarding the monitoring of construction workers’ activities necessitates an efficient means of continuous monitoring for timely action recognition at construction sites. This paper introduces a novel approach—the multi-scale graph strategy—to enhance feature extraction in complex networks. At the core of this strategy lies the multi-feature fusion network (MF-Net), which employs multiple scale graphs in distinct network streams to capture both local and global features of crucial joints. This approach extends beyond local relationships to encompass broader connections, including those between the head and foot, as well as interactions like those involving the head and neck. By integrating diverse scale graphs into distinct network streams, we effectively incorporate physically unrelated information, aiding in the extraction of vital local joint contour features. Furthermore, we introduce velocity and acceleration as temporal features, fusing them with spatial features to enhance informational efficacy and the model’s performance. Finally, efficiency-enhancing measures, such as a bottleneck structure and a branch-wise attention block, are implemented to optimize computational resources while enhancing feature discriminability. The significance of this paper lies in improving the management model of the construction industry, ultimately aiming to enhance the health and work efficiency of workers.

Background. Arising from T progenitor cells, T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematologic malignant tumor, accounting for 15% of childhood ALL and 25% of adult ALL. Composing of putative enhancers in close genomic proximity, super enhancer (SE) is critical for cell identity and the pathogenesis of multiple cancers. Belonging to the cytosolute linker protein group, FYB1 is essential for TCR signaling and extensively studied in terms of tumor pathogenesis and metastasis. Dissecting the role of FYN binding protein 1 (FYB1) in T-ALL holds the potential to improve the treatment outcome and prognosis of T-ALL. Methods. In this study, SEs were explored using public H3K27ac ChIP-seq data derived from T-ALL cell lines, AML cell lines and hematopoietic stem and progenitor cells (HSPCs). Downstream target of FYB1 gene was identified by RNA-seq. Effects of shRNA-mediated downregulation of FYB1 and immunoglobulin lambda-like polypeptide 1 (IGLL1) on self-renewal of T-ALL cells were evaluated in vitro and/or in vivo. Results. As an SE-driven gene, overexpression of FYB1 was observed in T-ALL, according to the Cancer Cell Line Encyclopedia database. In vitro, knocking down FYB1 led to comprised growth and enhanced apoptosis of T-ALL cells. In vivo, downregulation of FYB1 significantly decreased the disease burden by suppressing tumor growth and improved survival rate. Knocking down FYB1 resulted in significantly decreased expression of IGLL1 that was also an SE-driven gene in T-ALL. As a downstream target of FYB1, IGLL1 exerted similar role as FYB1 in inhibiting growth of T-ALL cells. Conclusion. Our results suggested that FYB1 gene played important role in regulating self-renewal of T-ALL cells by activating IGLL1, representing a promising therapeutic target for T-ALL patients.

Abstract To accurately identify fatigued driving, establishing a monitoring system is one of the important guarantees of improving traffic safety and reducing traffic accidents. Among many research methods, electrooculogram signal (EOG) has unique advantages. This paper presents a systematic literature review of these technologies and summarizes a basic framework of fatigue driving monitoring system based on EOGs. Then we summarize the advantages and disadvantages of existing technologies. In addition, 80 primary references published during the last decade were identified. The multi-feature fusion technique based on EOGs performs better than other traditional methods due to its low cost, low power consumption and low intrusion, while its application is still limited which needs more efforts to obtain good and generalizable results. And then, an overview of the literature on technology is given, revealing a premier and unbiased survey of the existing empirical research of classification techniques that have been applied to fatigue driving analysis. Finally, this paper adds value to the current literature by investigating the application of EOG signals in fatigued driving and the design of related systems, future guidelines have been provided to practitioners and researchers to grasp the major contributions and challenges in the state-of-the-art research.

Utilizing the heat energy of exhaust gases is a promising application of thermoelectric generator (TEG), which can convert low grade thermal energy into electricity. The annular thermoelectric generator (ATEG) is thought to be much more feasible for cylindrical heat source compared to the flat-plate thermoelectric generator (FTEG) in reference to the compatibility. Nevertheless, the quantitative comparison is lacking to clarify the prevalent geometry of TEG for cylindrical heat source. In this work, the performances of ATEG and FTEG are compared in detail with varying inlet temperature, velocity and the convective heat transfer coefficient when cylindrical heat source is applied. The turbulent heat source is described by the standard κ-ɛ functions together with the two-equation heat transfer model, while the output power and conversion efficiency of the ATEG and FTEG are calculated by solving the coupled thermo-electric equations. Our results showed that the output powers and the conversion efficiencies of ATEG and FTEG both increase with the increase of the inlet velocity, temperature, and the convective heat transfer coefficient. The conversion efficiency of ATEG is always higher than that of FTEG. The conversion efficiency of ATEG becomes even larger than that of FTEG when inlet temperature and/or convective heat transfer coefficient are relatively larger. In contrast, the output power of ATEG has no obvious difference with that of FTEG. This study addressed the condition when the ATEG has obvious advantage compared to the FTEG with cylindrical heat source applied. Our results suggest the annular TEG is better choice for cylindrical hot source especially when the inlet temperature and/or convective heat transfer coefficient are relatively larger. It could be a helpful guide for choosing suitable geometry of TEGs for energy harvesting in complex condition.

Abstract NF-κB signaling pathway is a critical link between inflammation and cancer. Emerging evidence suggested that long non-coding RNAs (lncRNAs) were involved in dysregulation of NF-κB. Herein, we reported a novel lncRNA IKBKBAS that activated NF-κB in lung adenocarcinoma (LUAD) by upregulating IKKβ, a key member of NF-κB signaling pathway, thereby promoting the metastasis of LUAD both in vitro and in vivo. The upregulated IKBKBAS functioned as a competing endogenous RNA (ceRNA) via competing with IKKβ mRNA for binding miR-4741, consequently leading to upregulation and activation of IKKβ, and ultimately activation of NF-κB. The abnormally elevated IKBKBAS in LUAD was mainly resulted from the extremely decrease of miR-512-5p that targeting IKBKBAS. Furthermore, we identified a positive feedback loop between NF-κB and IKBKBAS, in which NF-κB activation induced by overexpression of IKBKBAS could promote the transcription of IKBKBAS by binding the κB sites within IKBKBAS promoter. Our studies revealed that IKBKBAS was involved in the activation of NF-κB signaling by upregulating the expression of IKKβ, which made it serve as a potential novel target for therapies to LUAD.

Background: Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) based on granulocyte colony-stimulating factor plus anti-thymocyte regimens (‘Beijing Protocol’) provides a salvage treatment for patients of acquired severe aplastic anemia (SAA) in China. However, graft- versus -host disease (GVHD) is a major impediment of haplo-HSCT due to human leukocyte antigen disparity. Recently, haplo-HSCT combined with umbilical cord blood (UCB) (haplo-cord HSCT) is performed in clinical trials to potentially reduce the risk of severe GVHD. Nevertheless, studies comparing GVHD in pediatric patients receiving haplo and haplo-cord HSCT for SAA are limited. Objective: The objective of this study was to investigate the impact of UCB co-infusion on GVHD in pediatric patients receiving haplo-HSCT for SAA. Design: We conducted a retrospective study of 91 consecutive SAA children undergoing haploidentical transplantation based on the ‘Beijing Protocol’ with or without co-infusion of UCB in our center. Methods: All patients received uniform non-myeloablative conditioning and GVHD prophylaxis. We compared baseline characteristics and transplant outcomes between the haplo ( n = 35) and haplo-cord ( n = 56) recipients. Results: All 91 patients achieved hematopoietic recovery from haploidentical donors, with a higher incidence of peri-engraftment syndrome observed with the haplo-cord group as compared with the haplo group (75.0% versus 48.6%, p = 0.029). Notably, the haplo-cord group showed a lower incidence of II–IV acute GVHD (aGVHD) than the haplo group (16.1% versus 42.9%, p = 0.002). Observed incidences of chronic GVHD (cGVHD) and moderate to severe cGVHD in the haplo-cord group were also lower than that in the haplo group (25.6% versus 51.3%, p = 0.019; 16.2% versus 41.3%, p = 0.016, respectively). Haplo-cord HSCT was identified as the only factor associated with a lower incidence of II–IV aGVHD and cGVHD in multivariate analysis. However, no differences were observed between the two groups for infections and survival outcomes. Conclusion: Our data indicated that co-infusion of UCB in ‘Beijing Protocol’-based haplo-HSCT may be effective for reducing the risk of severe GVHD in SAA children.

Several studies indicated that the gut microbiota might participate in the beneficial effect of inulin on obesity. However, the mechanisms involved were still largely unknown. Sixteen high-fat diets (HFDs)-induced obese C57BL/6 mice were converted to a normal diet and then randomized into two groups, OND (obese mice + normal diet) group gavage-fed for 10 weeks with normal saline and ONDI (obese mice + normal diet + inulin) group with inulin at 10 g/kg/day. The body weight of HFD-induced obese mice showed different degrees of decrease in both groups. However, the ONDI group lost more weight and returned to normal earlier. Compared to the OND group, inulin supplementation significantly shifted the composition and structure of gut microbiota, such as higher α diversity. The β diversity analysis also confirmed the changes in gut microbiota composition between groups. At the genus level, the abundance of Alistipes was considerably increased, and it was significantly correlated with inulin supplementation (r = 0.72, P = 0.002). Serum metabolite levels were distinctly altered after inulin supplementation, and 143 metabolites were significantly altered in the ONDI group. Among them, indole-3-acrylic acid level increased more than 500-fold compared to the OND group. It was also strongly positive correlation with Alistipes (r = 0.72, P = 0.002) and inulin supplementation (r = 0.99, P = 9.2e−13) and negatively correlated with obesity (r = −0.72, P = 0.002). In conclusion, inulin supplementation could accelerate body weight loss in obese mice by increasing Alistipes and indole-3-acrylic acid level.

Apoptosis-related speck-like protein (ASC) is a key adapter protein in many inflammasome protein complexes, which mediates inflammation and host defense. In this study, an ASC gene (named Msasc) was identified and cloned from the liver tissue of Micropterus salmoides. The full-length cDNA sequence of Msasc was 573 bp, encoding 190 amino acids. Protein prediction shows that MsAsc contains two conserved domains, including the pyrin domain and caspase recruitment domain. The tissue distribution of Msasc mRNA in M. salmoides was investigated using the qPCR method. The expression of this gene could be detected in eight types of tissues, among which the heart, gill, and liver tissues showed a relatively higher expression level; the expression level was the lowest in the brain. When infected with Aeromonas veronii, the expression of Msasc in the spleen and liver tissues of M. salmoides reached its peak at 24 h after infection. Cell transfection experiment showed that MsAsc was distributed in HeLa cells in a diffuse manner. Results of the immune challenge experiment revealed that the inflammasome stimulator nigericin could markedly induce the aggregation of MsAsc in HeLa cells. These results suggest that Msasc is involved in the immune response of M. salmoides to A. veronii infection. This study lays a foundation for an in-depth understanding of the antibacterial function of Msasc in M. salmoides.

Acute myeloid leukemia (AML) represents an aggressive hematopoietic malignancy with a prognosis inferior to that of other leukemias. Recent targeted therapies offer new opportunities to achieve better treatment outcomes. However, due to the complex heterogeneity of AML, its prognosis remains dismal. In this study, we first identified the correlation between high expression of BRD4 and overall survival of patients with AML. Targeted degradation of BRD2, BRD3, and BRD4 proteins by dBET1, a proteolysis-targeting chimera (PROTAC) against the bromodomain and extra-terminal domain (BET) family members, showed cytotoxic effects on Kasumi (AML1-ETO), NB4 (PML-RARa), THP-1 (MLL-AF9), and MV4-11 (MLL-AF4) AML cell lines representing different molecular subtypes of AML. Furthermore, we determined that dBET1 treatment arrested cell cycling and enhanced apoptosis and c-MYC was identified as the downstream target. Collectively, our results indicated that dBET1 had broad anti-cancer effects on AML cell lines with different molecular lesions and provided more benefits to patients with AML.

The past decade has witnessed an increasing frequency and intensity of disasters, from extreme weather, drought, and wildfires to hurricanes, floods, and wars. Providing timely disaster response and humanitarian aid to these events is a critical topic for decision makers and relief experts in order to mitigate impacts and save lives. When a disaster occurs, it is important to acquire first-hand, real-time information about the potentially affected area, its infrastructure, and its people in order to develop situational awareness and plan a response to address the health needs of the affected population. This requires rapid assembly of multi-source geospatial data that need to be organized and visualized in a way to support disaster-relief efforts. In this paper, we introduce a new cyberinfrastructure solution—GeoGraphVis—that is empowered by knowledge graph technology and advanced visualization to enable intelligent decision making and problem solving. There are three innovative features of this solution. First, a location-aware knowledge graph is created to link and integrate cross-domain data to make the graph analytics-ready. Second, expert-driven disaster response workflows are analyzed and modeled as machine-understandable decision paths to guide knowledge exploration via the graph. Third, a scene-based visualization strategy is developed to enable interactive and heuristic visual analytics to better comprehend disaster impact situations and develop action plans for humanitarian aid.

Harmonics in electroencephalogram (EEG) caused by visual stimulation are the main basis of classification of steady-state visual evoked potential (SSVEP). However, the correlation of various harmonics, which could improve the classification performance especially when evoked EEG components are much weaker than spontaneous EEG components, has not been take into consideration in the design of classifier in previous studies. In this study, we proposed a filter bank convolutional neural network (FBCNN) method to optimize SSVEP classification. Three filters with passbands covering each harmonic of SSVEP signals are used to extract and separate the corresponding components, and the information from them are transformed into frequency domain. Subsequently, we introduce a novel convolutional neural network (CNN) architecture with three parallel CNN channels to extract and learn the harmonic features in passbands, and conclusions on the correlation among harmonics can finally be made by pair-add-up operations and dimension reductions to weigh the feature vectors. The proposed FBCNN is evaluated on two public datasets (Dataset1: 12-class, 10 subjects; Dataset2: 40-class, 35 subjects) to compare with other methods. The experimental results illustrate that FBCNN method improves the performance of CNN-based SSVEP classification methods and has a great potential to be applied in SSVEP-based BCI.

PurposeLong non-coding RNAs musculoaponeurotic fibrosarcoma oncogene family, protein G antisense 1 (lnc-MAFG-AS1) regulates hepatocellular carcinoma (HCC) progression and treatment resistance in multiple ways, while its engagement in HCC clinical management remains obscure. The current study aims to explore the relationship of lnc-MAFG-AS1 with tumor features, liver function indexes, tumor markers, and prognosis in HCC patients.MethodsOne hundred and fifty-two surgical HCC patients who underwent tumor resection were retrospectively analyzed. Their tumor and adjacent tissues were acquired and then proposed to reverse transcription-quantitative polymerase chain reaction to detect lnc-MAFG-AS1 expression.ResultsLnc-MAFG-AS1 expression was increased in HCC tumor tissue than in adjacent tissue [median (interquartile range): 2.730 (1.685–4.198) vs. 0.990 (0.703–1.468), p 0.05). In addition, patients with a high expression of lnc-MAFG-AS1 exhibited worse overall survival than those with a low expression of lnc-MAFG-AS1 [median (95% CI): 34.0 (24.5–43.5) vs. 48.0 (41.5–54.5) months] (p = 0.011), which was further validated by univariate Cox’s analysis [hazard ratio (HR) = 1.827, p = 0.013] and multivariate Cox’s analysis (HR = 1.697, p = 0.040).ConclusionLnc-MAFG-AS1 relates to multifocal nodules, increased BCLC stage, elevated CNLC stage, and abnormal AFP level and predicts pejorative prognosis in HCC patients.

Siniperca chuatsi feeds on live fry throughout their life. The sustainable development of its farming industry has urgently necessitated the development of artificial diets to substitute live baits. It has been demonstrated that gut microbiota assists in feed adaptation and improves the feed conversion rate in fish. Therefore, this study aimed to understand the potential role of intestinal microorganisms in the domestication of S. chuatsi with a compound diet. Accordingly, we performed 16S rRNA sequencing of the gut microbial communities in S. chuatsi groups that were fed a compound diet (including large and small individuals) and live baits. A total of 2,471 OTUs were identified, and the large individual group possessed the highest number of unique OTUs. The α-diversity index of the gut microbiota in groups that were fed a compound diet was significantly higher (p < 0.05) than that in the live bait group. There were no significant differences in the α-diversity between the large and small individual groups. However, relatively higher numbers of Lactococcus, Klebsiella, and Woeseia were observed in the intestines of the large individual group. Prediction of the metabolic function of the microbiota among these three fish groups by Tax4Fun revealed that most metabolic pathways, such as glycan metabolism and amino acid metabolism, were typically more enriched for the larger individuals. The results indicated that certain taxa mentioned above exist in large individuals and may be closely related to the digestion and absorption of compound diets. The present study provides a basis for understanding the utilization mechanism of artificial feed by S. chuatsi.

ABSTRACT The CD2-like (CD2V) protein is a crucial antigen of African swine fever virus (ASFV). CD2V interacts with the cellular AP-1 protein, participates in intracellular transport of virus, and induces neutralizing antibodies to partly protect swine from virus attack. In this study, a specific CD2V dimeric protein was designed to enhance antigenicity and immunogenicity, expressed in a Bac-to-Bac baculovirus expression vector system and purified by Ni-affinity chromatography. After animal immunization, five monoclonal antibodies (mAbs) (7E12, 22B3, 18A3, 13G11, and 43C2) against CD2V were developed. The variable regions of heavy chains and light chains of the mAbs were sequenced to prove that the five mAbs differed from one another. The mAbs of CD2V could combine with ASFV by immunoperoxidase monolayer assay (IPMA). B cell epitopes of CD2V were screened using the five mAbs by indirect enzyme-linked immunosorbent assay (ELISA) and Dot-ELISA. Therefore, three B cell epitopes (147FVKYT151, 157EYNWN161, and 195SSNY198) were identified. This is the first time that mAbs of the ASFV CD2V protein have been developed and the sequencing of heavy chains and light chains of mAbs has been completed. Linear B cell epitopes, which were core targets of immunoprotection of the CD2V protein, were identified by mAbs for the first time. This study provides efficient epitopes for the development of ASFV subunit vaccines. IMPORTANCE The ASFV CD2V protein is a crucial antigen on the outer envelopes of virus particles. A modified ASFV CD2V dimeric protein was expressed in the Bac-to-Bac baculovirus expression vector system. Five monoclonal antibodies (mAbs) against CD2V were developed, sequenced, and applied to identify CD2V protein B cell epitopes. Three B cell epitopes, 147FVKYT151, 157EYNWN161, and 195SSNY198, were identified. This is the first time CD2V mAbs have been developed, the sequencing of heavy chains and light chains of CD2V mAbs have been completed, and CD2V B cell epitopes have been identified by using scanning peptide method and bioinformatics methods.

Heat storage with phase change materials (PCMs) is promising to enhance the performance of thermoelectric generator (TEG) systems. Optimizing the thermal management in practical application is crucial to improve the performance of these TEG-PCM coupling systems. In this study, a TEG system coupled with PCMs has been fabricated and thermal interface materials (TIMs) with different thermal conductivities were used to regulate the interfacial thermal conduction between heat storage unit, TEG and heat exchanger. The experimental results revealed that the interfacial thermal conduction has substantial effects on the temperatures and performance of the TEG system coupled with PCMs. With the improvement of the interfacial thermal conduction, the time of the temperature of PCMs maintaining stable during cooling (tp) was shortened, while the average of output power of TEG system within this period (Pp) was increased. It indicates that the excess energy generated with the coupled PCMs would not increase monotonously with the enhancement of interfacial thermal conduction. We proposed a method to explore the optimal interfacial thermal conduction for the largest excess energy by performing polynomial fitting analysis of tpand Pp, respectively. This study provided a feasible means to improve the performance of TEG-PCM coupling systems by regulating the interfacial thermal conduction.

Sleep stage classification, including wakefulness (W), rapid eye movement (REM), and non- rapid eye movement (NREM) which includes three sleep stages that describe the depth of sleep, is one of the most critical steps in effective diagnosis and treatment of sleep-related disorders. Clinically, sleep staging is performed by domain experts through visual inspection of polysomnography (PSG) recordings, which is time-consuming, labor-intensive and often subjective in nature. Therefore, this study develops an automatic sleep staging system, which uses single channel electroencephalogram (EEG) signal, for convenience of wearing and less interference in the sleep, to do automatic identification of various sleep stages. To achieve the automatic sleep staging system, this study proposes a two-layer stacked ensemble model, which combines the advantages of random forest (RF) and LightGBM (LGB), where RF focuses on reducing the variance of the proposed model while LGB focuses on reducing the bias of the proposed model. Particularly, the proposed model introduces a class balance strategy to improve the N1 stage recognition rate. In order to evaluate the performance of the proposed model, experiments are performed on two datasets, including Sleep-EDF database (SEDFDB) and Sleep-EDF Expanded database (SEDFEDB). In the SEDFDB, the overall accuracy (ACC), weight F1-score (WF1), Cohen's Kappa coefficient (Kappa), sensitivity of N1 (SEN-N1) obtained by proposed model are 91.2%, 0.916, 0.864 and 72.52% respectively using subject-non-independent test (SNT). In parallel, the ACC, WF1, Kappa, SEN-N1 obtained by proposed model are 82.4%, 0.751, 0.719 and 27.15% respectively using subject-independent test (SIT). Experimental results show that the performance of the proposed model are competitive with the state-of-the-art methods and results, and the recognition rate of N1 stage is significantly improved. Moreover, in the SEDFEDB, the experimental results indicate the robustness and generality of the proposed model.

Despite the widespread use of plastic bellows in prestressed channels, their poor bonding with concrete and mortar results in “layering,” which limits their application in practical engineering. In this study, the bonding property between plastic bellows and concrete or grout material was investigated using a single-end compression test on plastic bellow concrete. The slip failure mode, ultimate load, and slip of plastic bellows were obtained. Furthermore, the analysis of the bond-slip properties of plastic bellows indicated that the bond strength decreased with increasing bond length. Moreover, the increase in the loading force was greater than the increase in the contact area. Based on the test data, a bond-slip constitutive relationship model was established, which accurately reflects the bond-slip process. The expressions of bond and slip were derived along with different bond positions of plastic bellow concrete specimens. Finally, a three-dimensional finite element model of a plastic bellow concrete specimen was established. The numerical simulation curve was compared with the experimental and fitting curves. The results indicated that the bond strength of the plastic bellow concrete specimen decreased with increasing bond length. The influence of bond strength on the contact area was comprehensively analyzed. This study effectively combines experimental research, theoretical analysis, and numerical simulation to analyze the bond performance between plastic bellows and concrete or grout material.

With the high demand for basic resources such as electricity, communication, water supply, and gas in cities, most cities in my country are vigorously developing underground integrated pipe gallery. When constructing pipe gallery in coastal areas, it is necessary to consider the impact of groundwater level on structural calculations. There is still a lack of systematic research in domestic and foreign literature. Relying on the Qingdao West Coast New Area Project, this paper uses a combination of laboratory tests and MIDAS GTS/NX numerical simulation to study the influence of groundwater in the coastal area on the stress and deformation characteristics of the integrated pipe gallery. The results show that (1) the Mises stress on the roof and bottom of the integrated pipe gallery increases continuously with the rise of the groundwater level. Due to the effect of the effective stress, the Mises stress on the side wall shows a decreasing trend. (2) Due to the influence of the construction form of the cross-section interface, the maximum principal stress of each part of the M side is always smaller than that of the N side; with the continuous increase of groundwater level, the maximum principal stress at each part of the N and M sides of the pipe gallery structure joint gradually increases. (3) The overall structure of the pipe gallery floats up. Under the action of the buoyancy force, the bottom plate moves upward, and the surface displacement rises continuously with the rise of the groundwater level. (4) When the groundwater depth is the same, the analysis of the test results and the numerical simulation calculation results are basically consistent, which further verifies the validity of the numerical simulation calculation and has reference value. (5) The engineering example shows that in order to reduce the service life of the integrated pipe gallery, the impact of groundwater on the integrated pipe gallery cannot be ignored.

Background. AML (acute myeloid leukemia) is a common hematological malignancy in children with poor treatment effects and poor prognosis. Recent studies have shown that as a novel BRD4 (bromodomain containing 4) PROTACs (proteolysis targeting chimeras) degrader, GNE-987 can slow down the growth of various tumors and increase apoptosis, with promising clinical prospects. However, the function and molecular mechanism of GNE-987 in AML remain unclear. This study is aimed at investigating the therapeutic effect of GNE-987 on AML and its underlying mechanism. Methods. The association between BRD4 and AML was assessed by studying public databases. After GNE-987 was added to AML cells, cell proliferation slowed down, the cycle was disturbed, and apoptosis increased. Western blotting was used to detect BRD2 (bromodomain containing 2), BRD3 (bromodomain containing 3), BRD4, and PARP (poly ADP-ribose polymerase) proteins. The effect of GNE-987 on AML cells was analyzed in vivo. RNA-seq (RNA sequencing) and ChIP-seq (chromatin immunoprecipitation sequencing) validated the function and molecular pathways of GNE-987 in processing AML. Results. BRD4 expression was significantly elevated in pediatric AML samples compared with healthy donors. GNE-987 inhibited AML cell proliferation by inhibiting the cell cycle and inducing apoptosis. BRD2, BRD3, and BRD4 were consistent with decreased VHL (Von Hippel Lindau) expression in AML cells. In an AML xenograft model, GNE-987 significantly reduced the hepatosplenic infiltration of leukemia cells and increased the mouse survival time. Based on analysis of RNA-seq and ChIP-seq analyses, GNE-987 could target multiple SE- (super-enhancer-) related genes, including LYL1 (lymphoblastic leukemia 1), to inhibit AML. Conclusions. GNE-987 had strong antitumor activity in AML. GNE-987 could effectively inhibit the expression of SE-related oncogenes including LYL1 in AML. Our results suggested that GNE-987 had broad prospects in the treatment of AML.

One of the advantages of sustainable competition for manufacturing systems is to make supply chain activities more efficient and effective. One of the major parts of these activities that can save a lot of costs is careful outsourcing. In this study, an approach based on decision-making policies in order to select suppliers and allocate order volume to them is introduced. The main contribution of this research is a comprehensive approach for optimizing both supplier evaluation and order allocation. In this regard, first, based on the evaluation, 39 key indicators were identified to evaluate the suppliers, and based on the content analysis, 25 key indicators were screened based on the Lavache method. Next, based on the fuzzy Delphi method, 11 indicators were selected from among the 25 key effective indicators. Finally, the Best-Worst Method (BWM) and a robust multi-objective formulation are proposed to find the weight of the effective criteria and the optimal order allocation to suppliers, respectively. Moreover, it is proved that the company under study faces a variety of suppliers and there is a need to analyze key indicators such as exchange rate changes and ease of communication. Based on the BWM results, it was shown that the exchange rate change trend with a weight of 0.24 is in the first place, and the quality system support index with a weight of 0.12 is in the second place. Moreover, based on the results of the proposed robust multi-objective mathematical model, it was revealed that in order to maintain the resilience of suppliers, the total demand should be distributed among all suppliers and the second supplier with a volume of 3600 units of raw materials has the largest share in delivering the required demand.

The use of polyenergy water pressure controlled blasting technology in tunnel construction is gradually being promoted, and the technology is often used in hard rock, and the mechanism of rock breaking in fractured sandstone strata is still lacking systematic research. The above mechanism was investigated using a combination of field experiments and ANSYS/LS-DYNA numerical simulation, and the results showed the following: (1) In the case of joint-hole blasting, the concentrating jet formed by the concentrating tube can effectively achieve controlled directional blasting of fractured sandstone. (2) The use of gun clay to seal the hole can effectively improve the stability of the blasting effect, reduce the waste of explosive gas, and effectively extend the duration of action. (3) The water bag enhances the blasting effect through the water wedge effect in the broken surrounding rock and also has the function of energy storage, which can effectively improve the blasting effect when combined with the gun clay. (4) The rock-breaking mechanism of polyenergy hydropressure smooth blasting in fractured sandstone geological conditions is the dual rock-breaking action of “polyenergy jet + water wedge action.” (5) In this article, based on the analysis of blasting mechanism, the deployment method of polyenergy hydropressure smooth blasting is designed and has been well applied in engineering practice.

The present paper investigates the relationship between pressure (bond stress) and displacement at the failure load for different materials through testing and modeling. First, single-end tests were conducted on prebonded stressed and perforated concrete specimens with corrugated plastic, metal, and rubber extractive pipes. These tests reveal that corrugated plastic pipes provide good bonding performance with concrete and grout. Hence, they can be applied in the hole-forming process of posttensioned prestressed bonded structures, along with corrugated metal pipes and rubber extractive pipes. Based on the experimental observations and results, a theoretical approach for applying corrugated plastic pipes in projects such as China’s high-speed rail has finally been demonstrated.

Propolis is resinous natural product produced by Western honeybees using beeswax and plant and bud exudates, which has a wide range of biological activities, including antioxidation, antibacterial, anti-inflammation, immune regulation, antitumor, and so on. Diffuse large B-cell lymphoma (DLBCL) is an aggressive cancer, and accounts for about 30% of all lymphomas. The effect of poplar propolis on DLBCL has not been reported. The IC50 of propolis on the proliferation of DLBCL SU-DHL-2 cell line and its proteins and gene expressions were detected by CCK-8 kit, label-free proteomic, and RT-PCR. The results showed that the IC50 of propolis at the 5 × l05/mL cell for 24 h was 5.729 μg/mL. Label-free-based proteomics analysis showed that there were 115 differentially expressed proteins (61 up-regulated and 54 down-regulated proteins) between IC50 dose-treated and solvent control groups. There were 32.47% differential proteins located in the nucleus, 20.78% in the cytoplasm, and 14.29% in mitochondria. The most significant different pathway (p = 0.0016) of protein enrichment was ferroptosis (including glutamate–cysteine ligase regulatory subunit, ferritin, and heme oxygenase). The relative expression trend of 17 of the total 22 genes selected according to proteomics results was in line with their encoded protein. The highest protein–protein interaction was serine/threonine-protein kinase PLK, which interacted with 16 differential proteins. In conclusion, poplar propolis inhibited SU-DHL-2 cells via ferroptosis pathway, accelerating cell death and down-regulated serine/threonine-protein kinase PLK1, affecting apoptosis of cell. This result provides a theoretical basis for the treatment of DLBCL using propolis.

Biochar-derived water-extractable organic matter (WEOM) was obtained under low-temperature pyrolysis (300 °C) using corncob as raw material. WEOM may affect the mobility and bioavailability of soil heavy metals (HMs) through complexation when biochar was used for soil HM remediation. Herein, the characteristics of complexation between HMs (Cr(III) and Cu(II)) and biochar-derived WEOM were investigated by using spectroscopic techniques in conjunction with parallel factor (PARAFAC) analysis and two-dimensional correlation spectroscopy (2D–COS). Six components were identified by PARAFAC modeling, in which protein-, fulvic- and humic-like components accounted for 48.86%, 25.63% and 25.51%, respectively. A nonlinear model was employed to determine the conditional stability constant (KM) and total ligand concentration (CL) of WEOM-HM complexes. The log KM values were in the range of 4.02–5.04 for WEOM-Cr(III) and 4.04–6.58 for WEOM-Cu(II). The 2D–COS in conjunction with log-transformed synchronous fluorescence spectroscopy (SFS) suggested that WEOM components were preferentially complexed with HMs in the following order: 433/270, 433/335, 496/270, 496/335, 370/335, 433/402, 496/402, 335/290, 402/290 for Cr(III), and 290/280, 390/280, 433/280, 496/280, 433/335, 496/335, 390/335, 433/420, 496/402, 335/290, 316/290 for Cu(II). The results of 2D–FTIR–COS suggested a preferential bonding of Cr(III) to the C–N group of alkyl, and Cu(II) to the CO group of alcohols, ethers and esters. Meanwhile, the CO group of ethers and the CN group of alkyl indicated preferential susceptibilities for the addition of Cr(III) and Cu(II) at different concentrations. In addition, protein-like components had remarkably higher total ligand concentration (CL) than fulvic- or humic-like components.

The ongoing COVID-19 pandemic caused by SARS-CoV-2 is a huge public health crisis for the globe. The receptor-binding domain (RBD) of SARS-CoV-2 spike (S) protein plays a vital role in viral infection and serves as a major target for developing neutralizing antibodies. In this study, the antibody response to the RBD of SARS-CoV-2 S protein was analyzed by a panel of sera from animals immunized with RBD-based antigens and four linear B-cell epitope peptides (R345, R405, R450 and R465) were revealed. The immunogenicity of three immunodominant peptides (R345, R405, R465) was further accessed by peptide immunization in mice, and all of them could induced potent antibody response to SARS-CoV-2 S protein, indicating that the three determinants in the RBD were immunogenic. We further generated and characterized monoclonal antibodies (15G9, 12C10 and 10D2) binding to these epitope peptides, and finely mapped the three immunodominant epitopes using the corresponding antibodies. Neutralization assays showed that all three monoclonal antibodies had neutralization activity. Results from IFA and western blotting showed that 12C10 was a cross-reactive antibody against both of SARS-CoV-2 and SARS-CoV. Results from conservative and structural analysis showed that 350VYAWN354 was a highly conserved epitope and exposed on the surface of SARS-CoV-2 S trimer, whereas 473YQAGSTP479 located in the receptor binding motif (RBM) was variable among different SARS-CoV-2 strains. 407VRQIAP412 was a highly conserved, but cryptic epitope shared between SARS-CoV-2 and SARS-CoV. These findings provide important information for understanding the humoral antibody response to the RBD of SARS-CoV-2 S protein and may facilitate further efforts to design SARS-CoV-2 vaccines and the target of COVID-19 diagnostic.