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Xyloglucan-block-polycaprolactone (XGO-PCL) copolymer nanoparticles have been proposed as nanocarriers for drug delivery. However, the possible harmful effects of exposure to nanoparticles still remain a concern. Therefore, the aim of this study is to evaluate the potential toxicity of XGO-PCL nanoparticles using in vitro and in vivo assays. Cytotoxicity and genotoxicity studies were conducted on MRC-5 human fetal lung fibroblast cells upon exposure to XGO-PCL nanoparticles. No significant reduction in the cell viability and no DNA damage were observed at the different concentrations tested. Erythrocyte toxicity was assessed by the incubation of nanoparticles with human blood. XGO-PCL nanoparticles induced a hemolytic ratio of less than 1%, indicating good blood compatibility. Finally, the subacute toxicity of XGO-PCL nanoparticles (10 mg/kg/day) was evaluated in BALB/c mice when administered orally or intraperitoneally for 14 days. Results of the in vivo toxicity study showed no clinical signs of toxicity, mortality, weight loss, or hematological and biochemical alterations after treatment with nanoparticles. Also, microscopic analysis of the major organs revealed no histopathological abnormalities, corroborating the previous results. Thus, it can be concluded that XGO-PCL nanoparticles induced no effect indicative of toxicity, indicating their potential use as drug delivery systems.

This work describes how nanostructured thin films obtained from the self-assembly of block copolymers (BCPs) systems can be used as a nanoelectrode array (NEA) that can be programmed to specifically detect targeted molecules. Namely, poly(styrene-b-methacrylate) (PS-b-PMMA) thin films, after removal of PMMA phase, produced regular spaced 560 pores μm-2 with 16 nm of diameter. The nanopores were then chemically modified by the introduction of β-cyclodextrin (β-CD) molecules. By using the supramolecular interaction of β-CD and ferrocene (Fc), the pores could be programed by the introduction of molecules linked to Fc able to interact with target species. In the model system shown here, the linker had a biotin unit, aiming the detection of streptavidin. By changing the linker, other molecules can also be detected. This concept opens a window to many possibilities, including the development of devices for fast and versatile molecule detection.

International audience; Original natural trio] (castor oil) was used as a monomer for the synthesis of monodisperse polyurethane nanoparticles (size ranging from 200 to 400 nm) by miniemulsion technique in water. Various parameters such as the concentration of the reactants, the nature of stabilizers and the shear were tuned to control the particle size and its distribution. The polyaddition between the natural triol and isophorone diisocyanate (IPDI) was conducted at 60 degrees C, in the absence of catalyst and monitored by infrared spectroscopy. The characterization of these polyurethane latexes was carried out using light scattering measurements and transmission electron microscopy (TEM).

Polycaprolactone (PCL) nanoparticles decorated with a mucoadhesive polysaccharide chitosan (CS) containing curcumin were developed aiming the buccal delivery of this drug. These nanoparticles were prepared by the nanoprecipitation method using different molar masses and concentrations of chitosan and concentrations of triblock surfactant poloxamer (PEO-PPO-PEO), in order to optimize the preparation conditions. Chitosan-coated nanoparticles showed positive surface charge and a mean particle radius ranging between 114 and 125 nm, confirming the decoration of the nanoparticles with the mucoadhesive polymer, through hydrogen bonds between ether and amino groups from PEO and CS, respectively. Dynamic Light Scattering (DLS) studies at different scattering angles and concentrations have shown that the nanoparticles are monodisperse (polydispersity indices were lower than 0.3). The nanoparticle systems were also examined with Nanoparticle Tracking Analysis (NTA), and the results were in good agreement with those obtained by DLS. Colloidal systems showed mean drug content about 460 μg/mL and encapsulation efficiency higher than 99%. Finally, when coated with chitosan, these nanoparticles show a great ability to interact with mucin indicating also their suitability for mucoadhesive applications.

International audience; The influence of surfactant type and concentration on particle size, formulation yield and stability of the polyurethane (PUR) nanoparticles synthesized by miniemulsion polymerization was investigated. SDS, Tween 80 and Pluronic F68 were employed as surfactants in concentration ranging from 5 to 20% (vs monomer concentration). The surfactant SDS was found not efficient in our system, resulting in a low formulation yield (about 53%), two particle size distributions and zeta potential equal to - 52.3mV. On the other hand, the nonionic surfactants gave monomodal particle size distribution, good yields (> 85%) and zeta potential around to- 24mV. The particles synthesized with Tween 80 or Pluronic F68 were very similar to each other in terms of efficiency, particle size distribution and zeta potential. For instance, the particle diameter slightly decreases from 292nm to 261nm as the amount of Tween 80 surfactant increases from 5 to 20wt.% vs monomer. Moreover, we have observed that a concentration of at least 5wt. % of Tween 80 was necessary to favor particle stability and therefore to avoid aggregation.

International audience; The morphology of micelles formed from blends of linear and cyclic poly(styrene-b-isoprene) (PS-b-PI) block copolymers has been investigated in solution using dynamic light scattering (DLS) and in thin solid deposits by atomic force microscopy (AFM) and transmission electron microscopy under cryogenic conditions (cryo-TEM). Micelles of the pure cyclic PS290-b-PI110 copolymers are wormlike cylindrical objects built by unidirectional aggregation of 33 nm wide sunflower micelles, while the linear block copolymer having the same volume fraction and molar mass forms spherical micelles 40 nm in diameter. The DLS, AFM, and cryo-TEM results consistently show that the addition of the linear copolymer (even for amounts as low as 5% w/w) to the cyclic copolymer rather favors the formation of spherical micelles at the expense of the cylindrical aggregates. Those results clearly show that the linear block copolymer chains can be used to stabilize the thermodynamically unstable elementary sunflower micelle. The thermal stability of the micelles (from the pure copolymers and from the blends) has been examined in solid deposits with in situ AFM measurements. Coalescence starts at about 70 degrees C, and the surface roughness shows a two-step decrease toward a fully homogeneous and flat structure.

International audience; We have studied the effect of polystyrene (PS) homopolymer addition on the morphology of self-assembled block copolymer micelles made from linear or cyclic poly(styrene-b-isoprene), PS-b-PI, in a selective solvent for the PI block (heptane). Both copolymers have the same composition: the degree of polymerization is 290 for the PS block, and 110 for the PI block, and we focused on the influence of the addition of small amounts of PS homopolymer on the micellar morphology. For the copolymer concentrations considered, the linear copolymer self-organizes into spherical micelles while the cyclic copolymer forms cylindrical micelles. PS and PI chains constitute the core and the corona of these micelles, respectively, due to the different affinity of the blocks for heptane. Consequently, the PS homopolymer added is "solubilized" into the micellar core. Dynamic light scattering (DLS) data combined with atomic force microscopy (AFM) results show that the addition of PS homopolymer induces a drastic change in the micellar organization. Indeed, a morphological transition, from spheres to cylinders for the linear copolymer, and from cylinders to vesicles for the cyclic copolymer, is observed. These results highlight the fact that a small incorporation of PS homopolymer is clearly sufficient to modify the morphology (size and shape) of the micelles. This approach could be a key parameter for the design/control of micelles for specific applications in nanotechnology.

International audience; We have studied the morphology of self-assembled micelles made of linear and cyclic poly(styrene-b-isoprene) PS-b-PI block copolymers dispersed in selective solvents of the PI block (n-heptane, n-decane). Up to a copolymer concentration of 5 mg mL(-1), the micelles made from linear block copolymer chains adopt a spherical shape. Those arising from cyclic copolymer chains having exactly the same molar mass and volume fraction self-assemble into (i) planar sun. ower-shaped particles at low concentration (c < 0.1 mg mL(-1)), (ii) giant wormlike micelles at intermediate concentration (0.1 mg mL(-1) < c < 2 mg mL(-1)) and (iii) vesicles at higher concentration (2 mg mL(-1) < c < 5 mg mL(-1)). Those results were obtained using dynamic light scattering and in situ freeze-drying cryo-transmission electron microscopy. In this contribution, we discuss the effects of concentration and temperature on the morphology of the self-assembled particles made from both linear and cyclic PS-PI copolymers, and highlight the surprising vesicle formation in cyclic block copolymer solutions.

International audience; Polyurethane nano- and microparticles were synthesized by suspension-polyaddition technique, using aqueous polymerization medium. Castor oil, a vegetable triglyceride possessing hydroxyl groups was used as natural polyol and methylene cliphenyl diisocyanate (MDI) as isocyanate. The levofloxacin, an antibacterial drug was used as model drug to measure the particles encapsulation efficiency. The effect of the addition of a second polyol, the poly(ethylene glycol) (PEG), and the stirring rate on the mean diameter and morphology of particles was also investigated. The poly(ethylene glycol) has an important effect in the reduction of particles size and their porosity. On the other hand, the poly(ethylene glycol) reduced the yield of encapsulation from 70% for the formulation without PEG to 20% for formulations with PEG. FTIR analysis confirmed the polyurethane formation. Dynamic light scattering study, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to determine the nanoparticles size and shape. Spectrofluorimetric analysis was used to detect the levofloxacin.

Made available in DSpace on 2021-06-25T11:48:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-09 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background: Bioelectrical impedance vector analysis (BIVA) is able to identify differences in hydration status and body composition components, such as body cell mass (BCM) by means of plotting individuals in ellipses, when comparing groups with different characteristics. Objective: Compare the confidence and tolerance ellipses of BIVA in individual and team sports athletes with a non-athlete reference population. Design and participants: One hundred sixty-seven college athletes (team sports: 117 athletes, individual sports: 50 athletes) aged between 18 and 35 years. Bioelectrical impedance was used to measure resistance (R) and reactance (Xc) values that were used in the BIVA analysis to identify hydration status and BCM, respectively. Hotelling's t-test was used to identify differences between groups in the confidence ellipses and the comparison was made with a non-athlete Italian reference population. Results: There were no differences between male team sports and individual athletes (p = 0.151) and for female athletes (p = 0.624). Most athletes were located in the 50% tolerance ellipses, indicating adequate hydration. Compared to the Italian a non-athlete population, athletes of both sexes presented left impedance vector deviation in the minor axis (Xc) of the tolerance ellipses, indicating higher BCM. Conclusion: There were no differences in BIVA between team and individual sports athletes, but most athletes presented an adequate hydration state and, compared to a non-athlete population, the athletes of the present study presented higher BCM. Univ Fed Santa Catarina, Dept Educ Fis, Ctr Desportos, Campus Univ Trindade Caixa Postal 476, BR-88040900 Florianopolis, SC, Brazil Univ Estadual Paulista, Sao Paulo, Brazil Univ Estadual Paulista, Sao Paulo, Brazil

International audience; The in vitro cytotoxicity and DNA damage evaluation of biodegradable polyurethane-based micro- and nanoparticles were carried out on animal fibroblasts. For cytotoxicity measurement and primary DNA damage evaluation, MTT and Comet assays were used, respectively. Different formulations were tested to evaluate the influence of chemical composition and physicochemical characteristics of particles on cell toxicity. No inhibition of cells growth surrounding the polyurethane particles was observed. On the other hand, a decrease of cell viability was verified when the anionic surfactant sodium dodecyl sulfate (SDS) was used as droplets stabilizer of monomeric phase. Polyurethane nanoparticles stabilized with Tween 80 and Pluronic F68 caused minor cytotoxic effects. These results indicated that the surface charge plays an important role on cytotoxicity. Particles synthesized from MDI displayed a higher cytotoxicity than those synthesized from IPDI. Size and physicochemical properties of the particles may explain the higher degree of DNA damage produced by two tested formulations. In this way, a rational choice of particles' constituents based on their cytotoxicity and genotoxicity could be very useful for conceiving biomaterials to be used as drug delivering systems.