9 results on '"Ricardo A. Escalona-Villalpando"'
Search Results
2. Immobilization of Glucose Oxidase on Glutathione Capped CdTe Quantum Dots for Bioenergy Generation
- Author
-
Daniel Lozano-López, Marisol Galván-Valencia, Ivone Rojas-de Soto, Ricardo A. Escalona-Villalpando, Janet Ledesma-García, and Sergio Durón-Torres
- Subjects
biofuel cells ,glucose oxidase ,quantum dots ,microfluidic ,enzymatic electrodes ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
An efficient immobilization of Glucose oxidase (GOx) on an appropriate substrate is one of the main challenges of developing fuel cells that allow energy to be obtained from renewable substrates such as carbohydrates in physiological environments. The research importance of biofuel cells relies on their experimental robustness and high compatibility with biological organisms such as tissues or the bloodstream with the aim of obtaining electrical energy even from living systems. In this work, we report the use of 5,10,15,20 tetrakis (1-methyl-4-pyridinium) porphyrin and glutathione capped CdTe Quantum dots (GSH-CdTeQD) as a support matrix for the immobilization of GOx on carbon surfaces. Fluorescent GSH-CdTeQD particles were synthesized and their characterization by UV-Vis spectrophotometry showed a particle size between 5–7 nm, which was confirmed by DLS and TEM measurements. Graphite and Toray paper electrodes were modified by a drop coating of porphyrin, GSH-CdTeQD and GOx, and their electrochemical activity toward glucose oxidation was evaluated by cyclic voltammetry, chronoamperometry and electrochemical impedance spectroscopy. Additionally, GOx modified electrode activity was explored by scanning electrochemical microscopy, finding that near to 70% of the surface was covered with active enzyme. The modified electrodes showed a glucose sensitivity of 0.58 ± 0.01 μA/mM and an apparent Michaelis constant of 7.8 mM. The addition of BSA blocking protein maintained the current response of common interferent molecules such as ascorbic acid (AA) with less than a 5% of interference percentage. Finally, the complex electrodes were employed as anodes in a microfluidic biofuel cell (μBFC) in order to evaluate the performance in energy production. The enzymatic anodes used in the μBFC allowed us to obtain a current density of 7.53 mAcm−2 at the maximum power density of 2.30 mWcm−2; an open circuit potential of 0.57 V was observed in the biofuel cell. The results obtained suggest that the support matrix porphyrin and GSH-CdTeQD is appropriate to immobilize GOx while preserving the enzyme’s catalytic activity. The reported electrode arrangement is a viable option for bioenergy production and/or glucose quantification.
- Published
- 2022
- Full Text
- View/download PDF
3. Comparative Colorimetric Sensor Based on Bi-Phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO Nanoparticles for Lactate Detection
- Author
-
Ricardo A. Escalona-Villalpando, Karen Viveros-Palma, Fabiola I. Espinosa-Lagunes, José A. Rodríguez-Morales, Luis G. Arriaga, Florika C. Macazo, Shelley D. Minteer, and Janet Ledesma-García
- Subjects
lactate colorimetric sensor ,bi-phase γ-/α-Fe2O3 ,peroxidase-like activity ,lactate oxidation ,bi-phase γ-/α-Fe2O3/ZnO ,Biotechnology ,TP248.13-248.65 - Abstract
This work reports on Fe2O3 and ZnO materials for lactate quantification. In the synthesis, the bi-phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO nanoparticles (NPs) were obtained for their application in a lactate colorimetric sensor. The crystalline phases of the NPs were analyzed by XRD and XPS techniques. S/TEM images showed spheres with an 18 nm average and a needle length from 125 to 330 nm and 18 nm in diameter. The γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO were used to evaluate the catalytic activity of peroxidase with the substrate 3,3,5,5-tetramethylbenzidine (TMB), obtaining a linear range of 50 to 1000 μM for both NPs, and a 4.3 μM and 9.4 μM limit of detection (LOD), respectively. Moreover, γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO/lactate oxidase with TMB assays in the presence of lactate showed a linear range of 50 to 1000 µM, and both NPs proved to be highly selective in the presence of interferents. Finally, a sample of human serum was also tested, and the results were compared with a commercial lactometer. The use of ZnO with Fe2O3 achieved a greater response toward lactate oxidation reaction, and has implementation in a lactate colorimetric sensor using materials that are economically accessible and easy to synthesize.
- Published
- 2022
- Full Text
- View/download PDF
4. Copper nanoparticles suitable for bifunctional cholesterol oxidation reaction: harvesting energy and sensor
- Author
-
F. I. Espinosa-Lagunes, J. C. Cruz, R. E. Vega-Azamar, I. Murillo-Borbonio, Julieta Torres-González, Ricardo A. Escalona-Villalpando, M. P. Gurrola, J. Ledesma-García, and L. G. Arriaga
- Subjects
Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Materials Chemistry ,Electronic, Optical and Magnetic Materials - Abstract
This study reports the performance of simple low-cost synthesized bifunctional Cu/Cu2O nanoparticles (NPs) used as a catalyst for energy-harvesting applications through of a microfluidic fuel cell (µFC), and further, as cholesterol (Chol) sensor. TEM characterization of the NPs showed spheres between 4 and 10 nm, while XRD and XPS analysis confirmed the composition and preferential crystallographic plane of Cu/Cu2O. In addition, 25.26 m2 g−1 surface area was obtained, which is greater than those commercial materials. NPs showed high activity toward the cholesterol oxidation reaction when were used as a sensor, obtaining a linear interval between 0.5 and 1 mM and 850 µA mM−1 mg−1 of sensitivity and 8.9 µM limit of quantification LOQ. These values are comparable to results previously reported. Moreover, Cu/Cu2O NPs were used as anode in a µFC with 0.96 V of cell voltage and 6.5 mA cm−2 and 1.03 mW cm−2 of current and power density, respectively. This performance is the highest currently reported for cholesterol application as an alternative fuel, and the first one reported for a microfluidic fuel cell system as far as is known. Results showed that the obtained Cu-based NPs presented an excellent performance for the dual application both µFC and sensor, which has potential applications in biomedicine and as an alternative energy source.
- Published
- 2022
5. Comparative Colorimetric Sensor Based on Bi-Phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO Nanoparticles for Lactate Detection
- Author
-
Ledesma-García, Ricardo A. Escalona-Villalpando, Karen Viveros-Palma, Fabiola I. Espinosa-Lagunes, José A. Rodríguez-Morales, Luis G. Arriaga, Florika C. Macazo, Shelley D. Minteer, and Janet
- Subjects
lactate colorimetric sensor ,bi-phase γ-/α-Fe2O3 ,peroxidase-like activity ,lactate oxidation ,bi-phase γ-/α-Fe2O3/ZnO - Abstract
This work reports on Fe2O3 and ZnO materials for lactate quantification. In the synthesis, the bi-phase γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO nanoparticles (NPs) were obtained for their application in a lactate colorimetric sensor. The crystalline phases of the NPs were analyzed by XRD and XPS techniques. S/TEM images showed spheres with an 18 nm average and a needle length from 125 to 330 nm and 18 nm in diameter. The γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO were used to evaluate the catalytic activity of peroxidase with the substrate 3,3,5,5-tetramethylbenzidine (TMB), obtaining a linear range of 50 to 1000 μM for both NPs, and a 4.3 μM and 9.4 μM limit of detection (LOD), respectively. Moreover, γ-/α-Fe2O3 and γ-/α-Fe2O3/ZnO/lactate oxidase with TMB assays in the presence of lactate showed a linear range of 50 to 1000 µM, and both NPs proved to be highly selective in the presence of interferents. Finally, a sample of human serum was also tested, and the results were compared with a commercial lactometer. The use of ZnO with Fe2O3 achieved a greater response toward lactate oxidation reaction, and has implementation in a lactate colorimetric sensor using materials that are economically accessible and easy to synthesize.
- Published
- 2022
- Full Text
- View/download PDF
6. A self-powered glucose biosensor device based on microfluidics using human blood
- Author
-
Ricardo A. Escalona-Villalpando, A. Sandoval-García, J. Roberto Espinosa L., M.G. Miranda-Silva, L.G. Arriaga, Shelley D. Minteer, and J. Ledesma-García
- Subjects
Detection limit ,Biosensor device ,biology ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Microfluidics ,Energy Engineering and Power Technology ,Signal ,Potentiostat ,Galvanostat ,biology.protein ,Glucose oxidase ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Biosensor ,Biomedical engineering - Abstract
A self-powered glucose biosensor (SPGB) was developed based on biofuel cells (BFCs)and a microfluidic system as a suitable technology for high potential application in clinical diagnosis. In this work, a bioanode and biocathode were assembled by glucose oxidase-based and laccase-based electrodes, respectively, which were incorporated into a microfluidic system (m-SPGB), while the anolyte and catholyte were phosphate buffered at different pH values of 7.4 and 5.6, respectively. The m-SPGB showed a linear dynamic interval of 0–10 mM (R2 = 0.9907), a low limit of detection of 0.48 mM and a response to interfering species. Subsequently, human blood was used in the anolyte to quantify glucose molecules in real samples, obtaining a low relative error percentage when compared with a commercial glucometer. The coupling of the m-SPGB with a wireless electronic device allowed the detection and processing of the signal in the quantification of glucose directly without using a potentiostat/galvanostat. The promising results of the m-SPGB connected to a Wi-Fi electronic device are well suited for clinical application.
- Published
- 2021
7. Desarrollo de bioánodos a partir de glucosa oxidasa (GOx) para su aplicación en biomicroceldas que usan glucosa como combustible
- Author
-
RICARDO ANTONIO ESCALONA VILLALPANDO
- Subjects
2 [cti] ,23 [cti] - Abstract
En el presente trabajo, se evaluaron tres formas diferentes de inmovilización de la enzima glucosa oxidasa (GOx) para su uso como bioánodo en una biocelda de combustible híbrida microfluídica (BCH-¿F) que utiliza glucosa como combustible. Los métodos de inmovilización probados fueron: adsorción física, por entrecruzamiento e inmovilización covalente de la GOx. La inmovilización física de la GOx se caracterizó por voltamperometría cíclica (VC) encontrándose un pico de oxidación en -0.44 V y otro de reducción a -0.49 V (vs Electrodo de Calomel Saturado ECS) pertenecientes al cofactor FAD/FADH2 (Flavín adenín dinucleótido) respectivamente. Por otro lado, los dos agentes entrecruzadores evaluados fueron glutaraldehído (GA), poli-L-lisina y una combinación de ambos, obteniendo con GA (4.5 %) una mayor cantidad de GOx inmovilizada con un recubrimiento de 2.6865 x 10-9 mol cm-2. Posteriormente, el depósito se optimizó con el uso de nanotubos de carbono de multipared (NTCMP) combinados con GOx y GA (1%), mejorando la transferencia directa de electrones (TDE), caracterizado por métodos electroquímicos y por FTIR (espectroscopia de infrarrojo con transformadas de Fourier). Mediante el modelo de Laviron se estimó el coeficiente de transferencia de electrones (¿) cuyo valor resultante fue de 0.5 y la constante de velocidad de la transferencia de electrones (Ks) fue de 1.01 s-1 para GOx/NTCMP-GA sobre grafito. Con el uso del bioánodo GOx/NTCMP-GA se obtuvo el mejor rendimiento dentro de una BCH-¿F utilizando Pt/C como cátodo en presencia de glucosa 5mM en BF, logrando un valor de potencial a circuito abierto de 0.72 V, 1.43 mA cm-2 de densidad de corriente y una potencia de 610 ¿W cm-2, valores mayores de los reportados en literatura para una BCH-¿F de TDE. Adicionalmente, la inmovilización de la GOx/NTCMP-GA se realizó sobre una estructura de oro electrodepositada sobre grafito logrando un valor de ¿=0.5 y Ks=2.1 s-1. Después se construyó un diseño de electrodo formado por el ácido 3-mercaptopropiónico anclado sobre la superficie de oro en presencia de EDC/NHS (1-etil-3-(3-dimetilaminopropil) carbodiimida)/N-hidroxisuccinamida) para favorecer la inmovilización covalente de la GOx. In this work, three different glucose oxidase (GOx) immobilization methods were evaluated to be used as a bioanode inside hybrid microfluidic biofuel cell (BCH-¿F) using glucose as fuel. The GOx immobilization methods were: physical adsorption, cross-linking and covalent. The physical immobilization of GOx was characterized by cyclic voltammetry (CV); a -0.44 V oxidation peak and a -0.49 V reduction peak (vs Saturated Electrode Calomel) belonging to the FAD/FADH2 (Flavin adenine dinucleotide) cofactor respectively, were found. On other hand, the two cross-linking agents evaluated were glutaraldehyde (GA), poly-L-lysine (PLL) and a combination of both. The highest GOx immobilization, with a 2.6865 x 10-9 mol cm-2 cover, was obtained through GA (4.5 %). Afterwards, the deposit was optimized with multiwall carbon nanotubes (MWCNT) combined with GOx and GA (1 %), which improved direct electron transfer (DET), characterized by electrochemical methods and FTIR (Fourier transform infrared spectroscopy). The coefficient electron transfer (¿) was estimated at 0.5 and the constant rate of electron transfer (Ks) was estimated at 1.01 s-1 via Laviron¿s model for GOx/MWCNT-GA on a graphite electrode. The best performance of BCH-¿F with the GOx/MWCNT-GA bioanode using Pt/C as a cathode in 5 mM of glucose in BF was obtained, resulting in an open circuit potential (OCP) of 0.72 V, current density at 1.43 mA cm-2 and power density at 610 ¿W cm-2; these results are the highest reported for DET GOx/MWCNT-GA. Additionally, GOx/MWCNT-GA was immobilization on architecture of golden electrodeposited on graphite carbon obtaining values of ¿=0.5 y Ks=2.1 s-1. Afterwards, 3-mercaptopropionic acid electrode was adsorbed on gold surface and modified by a mixture of EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/ N-hidroxisuccinamide) to promote covalent immobilization of GOx.
- Published
- 2014
8. Preparation of conductive carbon paper based on carbon nanofibers and polypyrrole for biofuel cell application.
- Author
-
Ricardo A Escalona-Villalpando, L G Arriaga, Shelley D Minteer, and J Ledesma-García
- Published
- 2018
- Full Text
- View/download PDF
9. Estudio de inmovilización enzimática, sobre sustratos de carbono modificados con Porfirina y Quantum dots para su empleo en celdas de combustible de carbohidratos
- Author
-
Lozano López, Jairo Daniel, Sergio Miguel Durón Torres, Marisol Galván Valencia, and Ricardo Antonio Escalona Villalpando
- Subjects
BIOLOGIA Y QUIMICA [2] ,carbohidratos ,sustratos de carbono ,inmovilización enzimática ,Porfirina ,Quantum - Abstract
The enzyme immobilization on conductive materials is a topic research for the development of biosensors and biofuel cells. The most common anchoring methods (covalent binding, cross-linking or inclusion in gels and membranes), often interfere with enzymatic catalysis due to active sites occlusion, enzyme denaturation or substrate diffusion problems. The use of Quantum dots (QD) as support matrix for the enzyme immobilization, has been reported as a viable alternative for the macromolecules anchoring, diminishing to a great extent the most well-known problems. In this work, a comparison between Graphene QD´s (GSH-CQD) and CdTe QD´s (GSH-CdTeQD) coated with glutathione is presented for the immobilization of the enzyme Glucose oxidase (GOx) as well as enzymes Lacasse (Lc), Glucose dehydrogenase (GDH) and β-galactosidase (Gal). The surfaces of graphite electrodes, carbon nanofoam and Toray paper was modified, with 5,10,15,20-Tetrakis (1-methyl-4-pyridinium) Porphyrin, a layer of QD was deposited to finally anchor GOx. The modified electrodes were evaluated by various electrochemical and physicochemical techniques. The results obtained show that QD´s modified electrodes have a greater immobilization capacity than graphite electrode and electrodes modified with GSH-CdTeQD are more sensitive to glucose. This suggests that the Porphyrin/GSH-CdTeQD/GOx system is suitable to be implemented in the development of a biofuel cell. La inmovilización de enzimas sobre materiales conductores, es un tema de investigación ampliamente estudiado para el desarrollo de biosensores y bioceldas de combustible. Los métodos más comunes para el anclaje de estas moléculas (enlace covalente, cross-linking, o inclusión en geles y membranas) a menudo interfieren con la catálisis enzimática debido a la oclusión de sitios activos, desnaturalización de la enzima o problemas de difusión del sustrato. El uso de Quantum dots como matriz de soporte para la inmovilización enzimática ha sido reportada como una alternativa viable para el anclaje de macromoléculas, disminuyendo en gran medida los problemas antes mencionados. En este trabajo se presenta una comparación entre Quantum dots de carbono (GSH-CQD) y de CdTe (GSH-CdTeQD) ambos recubiertos con glutatión (GSH), para la inmovilización de la enzima Glucosa oxidasa (GOx) así como de las enzimas Lacasa (Lc) Glucosa deshidrogenasa (GDH) y β-Galactosidasa (Gal). La superficie de electrodos de grafito, nanoesponja y papel de fibra de carbono (Toray) fue modificada con 5,10,15,20-Tetrakis (1-metil-4-piridinio) Porfirina, luego se depositó una capa de Quantum dots (GSH-CQD o GSH-CdTeQD) para finalmente anclar la GOx. Los electrodos modificados fueron evaluados por diversas técnicas tanto electroquímicas como fisicoquímicas. Los resultados obtenidos muestran que los GSH-CQD y GSH-CdTeQD tienen una mayor capacidad de inmovilización que el electrodo de grafito sin modificaciones sin embargo los electrodos modificados con GSH-CdTeQD son más sensibles a la glucosa. Esto sugiere que el sistema Porfirina/GSH-CdTeQD/GOx es adecuado para implementarse en el desarrollo de una biocelda de combustible.
- Published
- 2019
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.