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Edge computing brings artificial intelligence algorithms and graphics processing units closer to data sources, making autonomy and energy-efficient processing vital for their design. Approximate computing has emerged as a popular strategy for energy-efficient circuit design, where the challenge is to achieve the best tradeoff between design efficiency and accuracy. The essential operation in artificial intelligence algorithms is the general matrix multiplication (GEMM) operation comprised of matrix multiplication and accumulation. This paper presents an approximate general matrix multiplication (AGEMM) unit that employs approximate multipliers to perform matrix–matrix operations on four-by-four matrices given in sixteen-bit signed fixed-point format. The synthesis of the proposed AGEMM unit to the 45 nm Nangate Open Cell Library revealed that it consumed only up to 36% of the area and 25% of the energy required by the exact general matrix multiplication unit. The AGEMM unit is ideally suited to convolutional neural networks, which can adapt to the error induced in the computation. We evaluated the AGEMM units’ usability for honeybee detection with the YOLOv4-tiny convolutional neural network. The results implied that we can deploy the AGEMM units in convolutional neural networks without noticeable performance degradation. Moreover, the AGEMM unit’s employment can lead to more area- and energy-efficient convolutional neural network processing, which in turn could prolong sensors’ and edge nodes’ autonomy.

Simple Summary Honey bee colonies collect pollen from plants as a source of nutrients. Diverse diets comprising pollen from many different plant species are beneficial for honey bee colony health, because they contain a greater diversity of nutrients than monofloral diets of one plant species only. Here, we present the results of the COLOSS “CSI Pollen” study on the diversity of pollen collected by honey bee colonies. In this study, beekeepers acted as citizen scientists sampling and analyzing pollen collected by their own colonies. As a simple measure of diversity, beekeepers determined the number of different colors found in pollen samples that were collected in a coordinated and standardized way. The support of 750 beekeepers allowed the collection of information about almost 18,000 pollen samples from many European countries. We found that the pollen samples consistently comprised approximately six different colors in total, of which four colors were abundant. ‘Urban’ habitats or ‘artificial surfaces’ were associated with higher pollen color diversity. This investigation highlights seasonal- and land-use-related differences in the pollen supply for honey bees, which affects beekeeping and pollinator health. Determining pollen colors is a simple, useful technique for beekeepers to estimate pollen diversity. Abstract A diverse supply of pollen is an important factor for honey bee health, but information about the pollen diversity available to colonies at the landscape scale is largely missing. In this COLOSS study, beekeeper citizen scientists sampled and analyzed the diversity of pollen collected by honey bee colonies. As a simple measure of diversity, beekeepers determined the number of colors found in pollen samples that were collected in a coordinated and standardized way. Altogether, 750 beekeepers from 28 different regions from 24 countries participated in the two-year study and collected and analyzed almost 18,000 pollen samples. Pollen samples contained approximately six different colors in total throughout the sampling period, of which four colors were abundant. We ran generalized linear mixed models to test for possible effects of diverse factors such as collection, i.e., whether a minimum amount of pollen was collected or not, and habitat type on the number of colors found in pollen samples. To identify habitat effects on pollen diversity, beekeepers’ descriptions of the surrounding landscape and CORINE land cover classes were investigated in two different models, which both showed that both the total number and the rare number of colors in pollen samples were positively affected by ‘urban’ habitats or ‘artificial surfaces’, respectively. This citizen science study underlines the importance of the habitat for pollen diversity for bees and suggests higher diversity in urban areas.

Three-dimensional (3D) magnetic resonance microscopy (MRM) is a modality of magnetic resonance imaging (MRI) optimized for the best resolution. Metamorphosis of the Carniolan worker honey bee (Apis mellifera carnica) was studied in vivo under controlled temperature and humidity conditions from sealed larva until the emergence of an adult. The 3D images were analyzed by volume rendering and segmentation, enabling the analysis of body, tracheal system and gastrointestinal tract through volume time course. Fat content sensitivity enabled the analysis of flight muscles transformation during the metamorphosis by the signal histogram and gray level co-occurrence matrix (GLCM). Although the transformation during metamorphosis is well known, MRM enables an alternative insight to this process, i.e., 3D in vivo which has relatively high spatial and temporal resolutions. The developed methodology can easily be adapted for studying metamorphosis of other insects or any other incremental biological process on a similar spatial and temporal scale.

The high annual production and use of cerium(IV) oxide nanoparticles (nCeO2s) may lead to their atmospheric release and substantial deposition on plants. This poses a potential threat to pollinators. We investigated the effects of nCeO2-spiked food (2–500 mg L−1) on summer and winter honeybees (Apis mellifera carnica) after chronic 9 days' oral exposure. Acetylcholinesterase (AChE) and glutathione S-transferase (GST) activities were measured in different body compartments (heads, thoraces, and haemolymph). The activity of AChE was assessed in salt-soluble (SS) (containing soluble and membrane AChE) and detergent-soluble (DS) (predominantly membrane-bound AChE) fractions. Exposure of honeybees to nCeO2-spiked food had no significant effects on survival up to 500 mg L−1 (

The honey bee is among most important pollinators threatened by environmental pollution, pest control and potentially, by products of nanotechnologies. The aim of the current study was an analysis of the neurotoxic potential of ingested zinc oxide nanomaterials (ZnO NMs) or zinc ions (Zn(2+)) on honey bees. We analysed a variety of biomarkers, including metabolic impairment, feeding rate, and survival, as well as the activities of a stress-related enzyme glutathione S-transferase, and the neurotoxicity biomarker acetylcholinesterase. Acetylcholinesterase activity was found to be elevated in bees exposed to either of the tested substances. In addition, we observed increased feeding rate in the group treated with Zn(2+) but not with ZnO NMs or control group. The observed effects we relate primarily to Zn(2+) ions. Here we provide evidence that zinc ions either originating from Zn salt or Zn-based NPs have a neurotoxic potential and thus might contribute to colony survival.

Varroa destructor is one of the most common parasites of honey bee colonies and is considered as a possible co-factor for honey bee decline. At the same time, the use of pesticides in intensive agriculture is still the most effective method of pest control. There is limited information about the effects of pesticide exposure on parasitized honey bees. Larval ingestion of certain pesticides could have effects on honey bee immune defense mechanisms, development and metabolic pathways. Europe and America face the disturbing phenomenon of the disappearance of honey bee colonies, termed Colony Collapse Disorder (CCD). One reason discussed is the possible suppression of honey bee immune system as a consequence of prolonged exposure to chemicals. In this study, the effects of the neonicotinoid thiamethoxam on honey bee, Apis mellifera carnica, pupae infested with Varroa destructor mites were analyzed at the molecular level. Varroa-infested and non-infested honey bee colonies received protein cakes with or without thiamethoxam. Nurse bees used these cakes as a feed for developing larvae. Samples of white-eyed and brown-eyed pupae were collected. Expression of 17 immune-related genes was analyzed by real-time PCR. Relative gene expression in samples exposed only to Varroa or to thiamethoxam or simultaneously to both Varroa and thiamethoxam was compared. The impact from the consumption of thiamethoxam during the larval stage on honey bee immune related gene expression in Varroa-infested white-eyed pupae was reflected as down-regulation of spaetzle, AMPs abaecin and defensin-1 and up-regulation of lysozyme-2. In brown-eyed pupae up-regulation of PPOact, spaetzle, hopscotch and basket genes was detected. Moreover, we observed a major difference in immune response to Varroa infestation between white-eyed pupae and brown-eyed pupae. The majority of tested immune-related genes were upregulated only in brown-eyed pupae, while in white-eyed pupae they were downregulated.

Slovene heritage in beekeeping is based on knowledgeable bee management practice documented well in 18th century. Well described mating biology of honeybees was first detailed description of specific behavior of honeybees based on observation in well know subspecies of Carniolan bee. Later in 20th century special focus was on dance communication, which was described by von Frisch (1965) as a dance language. In the work alone it was not described as a special cognitive capability of the honeybees but rather instinctive highly specialized behavior pattern. From original work to nowadays many detailed studies of the dance communication were published and also used as a tool to study orientation and learning during foraging behavior. New concept have been involved, especially in sense of new views and methods in cognitive science. How much cognition is involved in foraging behavior of honeybees depends on how wide we accept definition of cognition. Key words : Apis mellifera carnica , communication, waggle dance, recruitment, foraging, information transfer Izvlecek Slovenska cebelarska dediscina temelji na dobrem poznavanju cebelarske prakse, ki je bila dokumentirana že v 18. stoletju. Dobro opisana biologija parjenja medonosne cebele je bil prvi podroben opis posameznega vedenja medonosne cebele temeljece na opazovanjih dobro poznane podvrste kranjske cebele. Kasneje v 20. stoletju je bila posebna pozornost na plesnem sporazumevanju cebel, ki jo je opisal von Frisch (1965) kot cebelji jezik. V samem njegovem delu sicer ni bilo opisano kot posebna razumska zmožnost cebel ampak bolj kot mocno usmerjen nagonski vedenjski vzorec. Od originalnega dela pa do danes je bilo objavljenih veliko podrobnih raziskav plesnega sporazumevanja in tudi uporabljeno kot orodje za raziskavo orientacije in ucenja med pasnim vedenjem. Razvili so se novi koncepti, se posebej v smislu novih pogledov in metod v kognitivni znanosti. Koliko razuma je vkljuceno v pasno vedenje cebel je odvisno od tega, kako na siroko sprejmemo definicijo razuma. Kljucne besede : Apis mellifera carnica , sporazumevanje, zibajoci ples, novacenje, pasa, prenos informacije

We observed social grooming behavior in the Carniolan bee, Apis mellifera carnica. Bouts of grooming lasted up to 45 s, and were directed to the wing axis (44.6%), the petiolus (18%) and the sternite regions of abdomen (2.8%) of the receiving bee (41 bees). During grooming, the receiving bees held their wings perpendicular to the body axis. Groomer bees most often cleaned those body parts which could not be reached during self-cleaning by receiving bees. During 18% of the grooming time, groomer bees cleaned their own mouth parts and antennae. The grooming behavior removed dust and pollen from the wing bases and petiolus and realigned the body hairs. No attempts to remove Varroa mites were observed during self-cleaning or social grooming behavior