In vivo continuous three-dimensional magnetic resonance microscopy: a study of metamorphosis in Carniolan worker honey bees (Apis mellifera carnica)

Three-dimensional (3D) magnetic resonance microscopy (MRM) is a modality of magnetic resonance imaging (MRI) optimized for the best resolution. Metamorphosis of the Carniolan worker honey bee (Apis mellifera carnica) was studied in vivo under controlled temperature and humidity conditions from sealed larva until the emergence of an adult. The 3D images were analyzed by volume rendering and segmentation, enabling the analysis of body, tracheal system and gastrointestinal tract through volume time course. Fat content sensitivity enabled the analysis of flight muscles transformation during the metamorphosis by the signal histogram and gray level co-occurrence matrix (GLCM). Although the transformation during metamorphosis is well known, MRM enables an alternative insight to this process, i.e., 3D in vivo which has relatively high spatial and temporal resolutions. The developed methodology can easily be adapted for studying metamorphosis of other insects or any other incremental biological process on a similar spatial and temporal scale.