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4. Antioxidant activity and qualitative analysis of wheat bran polyphenols purified using HPD 600 macroporous resin

Author

TIAN Fu-lin, HUANG Wen-jing, ZHANG Chi, SHEN Wang-yang, and WU Fan

Subjects
wheat bran polyphenols, macroporous resin, purify, antioxidant, hplc-ms/ms, qualitative analysis, Food processing and manufacture, TP368-456
Abstract

Objective: A process was developed for the purification of bran polyphenols from HPD 600 macroporous resin. The purity and antioxidant activity of the extracts were analyzed before and after purification, and the polyphenol fractions in the purified products were characterized. Methods: The bound polyphenols in wheat bran were extracted by alkali hydrolysis, and the dynamic adsorption-desorption conditions of the macroporous resin were optimized by single-factor test to determine the optimal purification process. The antioxidant activity fraction was determined by ABTS and DPPH radical scavenging rate. The characterization of the polyphenol fraction in the purified product was completed by UPLC-MS/MS. Results: Under optimal process conditions, the purity of the polyphenols and the antioxidant activity of the purified product were significantly increased. The purified products contained mainly nine polyphenolic compounds, in order of retention time: p-hydroxybenzoic acid, caffeic acid, vanillin, p-coumaric acid, trans-ferulic acid and salicylic acid. Conclusion: The macroporous resin purification process is effective in enriching bran polyphenols and the resulting purified product has a significantly higher antioxidant capacity, while maintaining the richness of the polyphenolic species.

Published
2022
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23. Overexpression of KRT17 promotes proliferation and invasion of non-small cell lung cancer and indicates poor prognosis

Author

Wang, Zhao, Yang, Mai-Qing, Lei, Lei, Fei, Liang-Ru, Zheng, Yi-Wen, Huang, Wen-Jing, Li, Zhi-Han, Liu, Chen-Chen, and Xu, Hong-Tao

Subjects
Wnt signaling pathway, keratin 17, Cancer Management and Research, epithelial mesenchymal transition, proliferation, invasion, non-small cell lung cancer, respiratory tract diseases, Original Research
Abstract

Zhao Wang,1,2 Mai-Qing Yang,1,3 Lei Lei,1 Liang-Ru Fei,1 Yi-Wen Zheng,1 Wen-Jing Huang,1 Zhi-Han Li,1 Chen-Chen Liu,1 Hong-Tao Xu11Department of Pathology, The First Hospital and College of Basic Medical Sciences of China Medical University, Shenyang 110001, People’s Republic of China; 2Department of Pathology, General Hospital of Heilongjiang Land Reclamation Bureau, Harbin 150088, People’s Republic of China; 3Department of Pathology, Changyi People’s Hospital, Changyi, People’s Republic of ChinaPurpose: Keratin 17 (KRT17) is a 48 KDa type I intermediate filament, which is mainly expressed in epithelial basal cells. KRT17 has been shown to be overexpressed in many malignant tumors and play an important role in the occurrence and development of tumors. Therefore, this study explored the role and underlying mechanism of KRT17 in non-small cell lung cancers (NSCLC).Methods: KRT17 expression and its correlations with clinicopathological factors were examined in lung cancer tissues by immunohistochemistry. The prognosis value of KRT17 in NSCLCs was retrieved from The Cancer Genome Atlas (TCGA) online databases. The expression level of KRT17 was increased or decreased by KRT17 gene transfection or small RNA interference in lung cancer cells, respectively. Further, proliferation and invasiveness of lung cancer cells were determined by cell proliferation and invasion assays, respectively. Finally, expression levels of proteins related to Wnt signaling pathways and epithelial mesenchymal transition (EMT) were detected by Western blot.Results: The expression level of KRT17 in NSCLCs was significantly higher than normal lung tissues. High expression of KRT17 predicted poor prognosis of patients with NSCLCs, especially lung adenocarcinomas, and was correlated with poor differentiation and lymphatic metastasis. Overexpression of KRT17 enhanced, while KRT17 knockdown inhibited, the proliferation and invasiveness of lung cancer cells. Overexpression of KRT17 up-regulated β-catenin activity and levels of Wnt target genes, such as cyclin D1, c-Myc, and MMP7. Moreover, KRT17 promoted EMT by up-regulating Vimentin, MMP-9, and Snail expression and down-regulating E-cadherin expression.Conclusion: Overexpression of KRT17 is common in NSCLCs and indicates poor prognosis. Overexpression of KRT17 enhances the proliferation and invasiveness of NSCLC cells by activating the Wnt signaling pathway and EMT process. KRT17 is a potential indicator of NSCLC progression and poor survival.Keywords: keratin 17, non-small cell lung cancer, Wnt signaling pathway, epithelial mesenchymal transition, proliferation, invasion

Published
2019

24. Additional file 1: of Molecular characterization of two novel reoviruses isolated from Muscovy ducklings in Guangdong, China

Author

Zhang, Xue-Lian, Shao, Jian-Wei, Li, Xiao-Wen, Mei, Min-Min, Jin-Yue Guo, Li, Wen-Feng, Huang, Wen-Jing, Chi, Shi-Hong, Yuan, Sheng, Li, Zhi-Li, and Shu-Jian Huang

Subjects
viruses, virus diseases
Abstract

Table S1. Oligonucleotide primers used to amplify and sequence the L/S/M-class genes of novel pathogen Muscovy duck reovirus (N-MDRV) SH12 and DH13. (DOCX 17 kb)

Published
2019
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32. Chinese herbal medicine TangBi Formula treatment of patients with type 2 diabetic distal symmetric polyneuropathy disease: study protocol for a randomized controlled trial

Author

Jin, De, primary, Huang, Wen-jing, additional, Meng, Xiang, additional, Yang, Fan, additional, Zheng, Yu-jiao, additional, Bao, Qi, additional, Zhang, Mei-zhen, additional, Yang, Ya-nan, additional, Ni, Qing, additional, Lian, Feng-mei, additional, and Tong, Xiao-lin, additional

Published
2017
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33. Structure Identification and In Vitro Anticancer Activity of Lathyrol-3-phenylacetate-5,15-diacetate

Author

Zhang, Jian-ye, primary, Huang, Wen-jing, additional, Sun, Hong-mei, additional, Liu, Yun, additional, Zhao, Xiao-qin, additional, Tang, Si-li, additional, Sun, Ming-na, additional, Wang, Sheng, additional, Li, Jia-jun, additional, Zhang, Ling-ling, additional, Zhou, Jun-hua, additional, Pan, Qian-rong, additional, and Chen, Hu-biao, additional

Published
2017
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34. World Congress Integrative Medicine & Health 2017: part two

Author

Ee, Carolyn, primary, Thuraisingam, Sharmala, additional, Pirotta, Marie, additional, French, Simon, additional, Xue, Charlie, additional, Teede, Helena, additional, Kristoffersen, Agnete E., additional, Sirois, Fuschia, additional, Stub, Trine, additional, Engler, Jennifer, additional, Joos, Stefanie, additional, Güthlin, Corina, additional, Felenda, Jennifer, additional, Beckmann, Christiane, additional, Stintzing, Florian, additional, Evans, Roni, additional, Bronfort, Gert, additional, Keefe, Daniel, additional, Taberko, Anna, additional, Hanson, Linda, additional, Haley, Alex, additional, Ma, Haiwei, additional, Jolton, Joseph, additional, Yarosh, Lana, additional, Keefe, Francis, additional, Nam, Jung, additional, Ojala, Liwanag, additional, Kreitzer, Mary J., additional, Fink, Careen, additional, Kraft, Karin, additional, Flower, Andrew, additional, Lewith, George, additional, Harman, Kim, additional, Stuart, Beth, additional, Bishop, Felicity L., additional, Frawley, Jane, additional, Füleki, Lilla, additional, Kiss, Eva, additional, Vancsik, Tamas, additional, Krenacs, Tibor, additional, Funabashi, Martha, additional, Pohlman, Katherine A., additional, Mior, Silvano, additional, Thiel, Haymo, additional, Hill, Michael D., additional, Cassidy, David J., additional, Westaway, Michael, additional, Yager, Jerome, additional, Hurwitz, Eric, additional, Kawchuk, Gregory N., additional, O’Beirne, Maeve, additional, Vohra, Sunita, additional, Gaboury, Isabelle, additional, Morin, Chantal, additional, Gaertner, Katharina, additional, Torchetti, Loredana, additional, Frei-Erb, Martin, additional, Kundi, Michael, additional, Frass, Michael, additional, Gallo, Eugenia, additional, Maggini, Valentina, additional, Comite, Mattia, additional, Sofi, Francesco, additional, Baccetti, Sonia, additional, Vannacci, Alfredo, additional, Di Stefano, Mariella, additional, Monechi, Maria V., additional, Gori, Luigi, additional, Rossi, Elio, additional, Firenzuoli, Fabio, additional, Mediati, Rocco D., additional, Ballerini, Giovanna, additional, Gardiner, Paula, additional, Lestoquoy, Anna S., additional, Negash, Lily, additional, Stillman, Sarah, additional, Shah, Prachi, additional, Liebschutz, Jane, additional, Adelstein, Pamela, additional, Farrell-Riley, Christine, additional, Brackup, Ivy, additional, Penti, Brian, additional, Saper, Robert, additional, Sampedro, Isabel Giralt, additional, Carvajal, Gilda, additional, Gleiss, Andreas, additional, Gross, Marie M., additional, Brendlin, Dorothea, additional, Röttger, Jonas, additional, Stritter, Wiebke, additional, Seifert, Georg, additional, Grzanna, Noelle, additional, Stange, Rainer, additional, Guendling, Peter W., additional, Gu, Wen, additional, Lu, Yan, additional, Wang, Jie, additional, Zhang, Chengcheng, additional, Bai, Hua, additional, He, Yuxi, additional, Zhang, Xiaoxu, additional, Zhang, Zhengju, additional, Wang, Dali, additional, Meng, Fengxian, additional, Hagel, Alexander, additional, Albrecht, Heinz, additional, Vollbracht, Claudia, additional, Dauth, Wolfgang, additional, Hagel, Wolfgang, additional, Vitali, Francesco, additional, Ganzleben, Ingo, additional, Schultis, Hans, additional, Konturek, Peter, additional, Stein, Jürgen, additional, Neurath, Markus, additional, Raithel, Martin, additional, Krick, Bianka, additional, Haller, Heidemarie, additional, Klose, Petra, additional, Dobos, Gustav, additional, Kümmel, Sherko, additional, Cramer, Holger, additional, Saha, Felix J., additional, Kowoll, Anna, additional, Ebner, Barbara, additional, Berger, Bettina, additional, Choi, Kyung-Eun, additional, He, Lisha, additional, Wang, Han, additional, He, X., additional, Gu, C., additional, Zhang, Y., additional, Zhao, Linhua, additional, Tong, Xiaolin, additional, He, Xinhui, additional, Gu, Chengjuan, additional, Zhang, Ying, additional, Ho, Robin S. T., additional, Chung, Vincent C. H., additional, Wu, Xinyin, additional, Wong, Charlene H. L., additional, Wu, Justin C. Y., additional, Wong, Samuel Y. S., additional, Lau, Alexander Y. L., additional, Sit, Regina W. S., additional, Wong, Wendy, additional, Holmes, Michelle, additional, Bishop, Felicity, additional, Calman, Lynn, additional, Newell, Dave, additional, Field, Jonathan, additional, Htut, Win L., additional, Han, Dongwoon, additional, Choi, Da I., additional, Choi, Soo J., additional, Kim, Ha Y., additional, Hwang, Jung H., additional, Huang, Ching W., additional, Jang, Bo H., additional, Chen, Fang P., additional, Ko, Seong G., additional, Huang, Wenjing, additional, Jin, De, additional, Lian, Fengmei, additional, Jang, Soobin, additional, Kim, Kyeong H., additional, Lee, Eun K., additional, Sun, Seung H., additional, Go, Ho Y., additional, Ko, Youme, additional, Park, Sunju, additional, Shin, Yong C., additional, Janik, Hubert, additional, Greiffenhagen, Natalie, additional, Bolte, Jürgen, additional, Jaworski, Mariusz, additional, Adamus, Miroslawa, additional, Dobrzynska, Aleksandra, additional, Jeitler, Michael, additional, Jaspers, Jessica, additional, von Scheidt, Christel, additional, Koch, Barbara, additional, Michalsen, Andreas, additional, Steckhan, Nico, additional, Kessler, Christian, additional, Huang, Wen-jing, additional, Pang, Bing, additional, Lian, Feng-Mei, additional, Jong, Miek, additional, Baars, Erik, additional, Glockmann, Anja, additional, Hamre, Harald, additional, Kainuma, Mosaburo, additional, Murakami, Aya, additional, Kubota, Toshio, additional, Kobayashi, Daisuke, additional, Sumoto, Yasuhiro, additional, Furusyo, Norihiro, additional, Ando, Shin-Ichi, additional, Shimazoe, Takao, additional, Kelber, Olaf, additional, Verjee, S., additional, Gorgus, Eva, additional, Schrenk, Dieter, additional, Kemper, Kathi, additional, Hill, Ellie, additional, Rao, Nisha, additional, Gascon, Gregg, additional, Mahan, John, additional, Kienle, Gunver, additional, Dietrich, Jörg, additional, Schmoor, Claudia, additional, Huber, Roman, additional, Kim, Weon H., additional, Ahmed, Mansoor, additional, He, Luzhu, additional, Hwang, Jung Hye, additional, Meggyeshazi, Nora, additional, Kovago, Csaba, additional, Klaus, Anne K., additional, Zerm, Roland, additional, Pranga, Danilo, additional, Ostermann, Thomas, additional, Reif, Marcus, additional, von Laue, Hans Broder, additional, Brinkhaus, Benno, additional, Kröz, Matthias, additional, Recchia, Daniela Rodrigues, additional, von Laue, Hans B., additional, Klein-Laansma, Christien T., additional, Jong, Mats, additional, von Hagens, Cornelia, additional, Jansen, Jean P., additional, van Wietmarschen, Herman, additional, Jong, Miek C., additional, Sun, Seung-Ho, additional, Go, Ho-Yeon, additional, Jeon, Chan-Yong, additional, Song, Yun-Kyung, additional, Ko, Seong-Gyu, additional, Koch, Anna K., additional, Rabsilber, Sybille, additional, Lauche, Romy, additional, Langhorst, Jost, additional, Trifunovic-Koenig, Milena, additional, Koster, Evi, additional, Delnoij, Diana, additional, Kroll, Lena, additional, Weiss, Kathrin, additional, Kubo, Ai, additional, Hendlish, Sarah, additional, Altschuler, Andrea, additional, Connolly, Nancy, additional, Avins, Andy, additional, Wardle, Jon, additional, Lee, David, additional, Sibbritt, David, additional, Adams, Jon, additional, Park, Crystal, additional, Mishra, Gita, additional, Lechner, Johann, additional, Lee, Inseon, additional, Chae, Younbyoung, additional, Lee, Jisu, additional, Cho, Seung H., additional, Choi, Yujin, additional, Lee, Jee Y., additional, Ryu, Han S., additional, Yoon, Sung S., additional, Oh, Hye K., additional, Hyun, Lyun K., additional, Kim, Jin O., additional, Yoon, Seong W., additional, Lee, Ju-Yeon, additional, Shin, Sang-Hoon, additional, Jang, Min, additional, Müller, Indra, additional, Park, So-Hyun Janson, additional, Laird, Lance, additional, Mitchell, Suzanne, additional, Li, Xiaofei, additional, Wang, Yunhui, additional, Zhen, Jianhua, additional, Yu, He, additional, Liu, Tiegang, additional, Gu, Xiaohong, additional, Liu, Hui, additional, Ma, Weiguo, additional, Shang, Xuezheng, additional, Bai, Yu, additional, Liu, Wei, additional, Rooney, Collin, additional, Smith, Amos, additional, Lopes, Shirlene, additional, Demarzo, Marcelo, additional, do Patrocínio Nunes, Maria, additional, Lorenz, Peter, additional, Gründemann, Carsten, additional, Heinrich, Miriam, additional, Garcia-Käufer, Manuel, additional, Grunewald, Franziska, additional, Messerschmidt, Silke, additional, Herrick, Anja, additional, Gruber, Kim, additional, Knödler, Matthias, additional, Steinborn, Carmen, additional, Lu, Taoying, additional, Wang, Lixin, additional, Wu, Darong, additional, Luberto, Christina M, additional, Hall, Daniel L., additional, Chad-Friedman, Emma, additional, Lechner, Suzanne, additional, Park, Elyse R., additional, Luberto, Christina M., additional, Park, Elyse, additional, Goodman, Janice, additional, Luer, Sonja, additional, Heri, Matthias, additional, von Ammon, Klaus, additional, Landini, Ida, additional, Lapucci, Andrea, additional, Nobili, Stefania, additional, Mini, Enrico, additional, McDermott, Clare, additional, Richards, Selwyn, additional, Cox, Diane, additional, Frossell, Sarah, additional, Leydon, Geraldine, additional, Eyles, Caroline, additional, Raphael, Hilly, additional, Rogers, Rachael, additional, Selby, Michelle, additional, Adler, Charlotte, additional, Allam, Jo, additional, Bu, Xiangwei, additional, Zhang, Honghong, additional, Zhang, Jianpeng, additional, Mikolasek, Michael, additional, Berg, Jonas, additional, Witt, Claudia, additional, Barth, Jürgen, additional, Miskulin, Ivan, additional, Lalic, Zdenka, additional, Miskulin, Maja, additional, Dumic, Albina, additional, Sebo, Damir, additional, Vcev, Aleksandar, additional, Mohammed, Nasr A. A., additional, Choi, Soo Jeung, additional, Im, Hyea Bin, additional, Mukherjee, Anwesha, additional, Kandhare, Amit, additional, Bodhankar, Subhash, additional, Thakurdesai, Prasad, additional, Munk, Niki, additional, Evans, Erica, additional, Froman, Amanda, additional, Kline, Matthew, additional, Bair, Matthew J., additional, Musial, Frauke, additional, Alræk, Terje, additional, Hamre, Harald J., additional, Björkman, Lars, additional, Fønnebø, Vinjar M., additional, Lian, Feng-mei, additional, Ni, Qing, additional, Tong, Xiao-lin, additional, Li, Xin-long, additional, Liu, Wen-ke, additional, Feng, Shuo, additional, Zhao, Xi-yan, additional, Zheng, Yu-jiao, additional, Zhao, Xue-min, additional, Lin, Yi-qun, additional, Zhao, Tian-yu, additional, Zhao, Xi-Yan, additional, Phd, Hui Che, additional, Zhang, Chen, additional, Liu, Feng, additional, Zhao, Lin-hua, additional, Ye, Ru, additional, Gu, Cheng-juan, additional, Peng, Wenbo, additional, De Carvalho, Diana, additional, El-Bayoumi, Mohamed, additional, Haig, Bob, additional, Kelly, Kimbalin, additional, Wade, Darrell J., additional, Portalupi, Emanuela, additional, Gobo, Giampietro, additional, Bellavita, Luigi, additional, Guglielmetti, Chiara, additional, Raak, Christa, additional, Teuber, Myriam, additional, Molsberger, Friedrich, additional, von Rath, Ulrich, additional, Reichelt, Ulrike, additional, Schwanebeck, Uta, additional, Zeil, Sabine, additional, Vogelberg, Christian, additional, Veintimilla, Dolores Rodríguez, additional, Mery, Guerrero Tapia, additional, Villavicencio, Marisol Maldonado, additional, Moran, Sandra Herrera, additional, Sachse, Christian, additional, Gündlin, Peter W, additional, Sahebkarkhorasani, Monirsadat, additional, Azizi, Hoda, additional, Schumann, Dania, additional, Sundberg, Tobias, additional, Leach, Matthew J., additional, Seca, Susana, additional, Greten, Henry, additional, Selliah, Sugir, additional, Shakya, Anu, additional, Kim, Ha Yun, additional, Im, Hyea B., additional, Sherbakova, Anna, additional, Ulrich-Merzenich, Gudrun, additional, Abdel-Aziz, Heba, additional, Sibinga, Erica, additional, Webb, Lindsey, additional, Ellen, Jonathan, additional, Skrautvol, Kari, additional, Nåden, Dagfinn, additional, Song, Rhayun, additional, Grabowska, Weronika, additional, Osypiuk, Kamila, additional, Diaz, Gloria V., additional, Bonato, Paolo, additional, Park, Moonkyoung, additional, Hausdorff, Jeffrey, additional, Fox, Michael, additional, Sudarsky, Lewis R., additional, Tarsy, Daniel, additional, Novakowski, James, additional, Macklin, Eric A., additional, Wayne, Peter M., additional, Hwang, Inok, additional, Ahn, Sukhee, additional, Lee, Myung-Ah, additional, Sohn, Min K., additional, Sorokin, Oleg, additional, Heydeck, Dagmar, additional, Borchert, Astrid, additional, Hohmann, Christoph-Daniel, additional, Kühn, Harmut, additional, Kirschbaum, Clemens, additional, Stalder, Tobias, additional, Stöckigt, Barbara, additional, Teut, Michael, additional, Suhr, Ralf, additional, Sulmann, Daniela, additional, Streeter, Chris, additional, Gerbarg, Patrica, additional, Silveri, Marisa, additional, Brown, Richard, additional, Jensen, John, additional, Rutert, Britta, additional, Eggert, Angelika, additional, Längler, Alfred, additional, Holmberg, Christine, additional, Sun, Jin, additional, Deng, Xin, additional, Li, Wen-Yuan, additional, Wen, Bin, additional, Robinson, Nicola, additional, Liu, Jian-Ping, additional, Sung, Hyun K., additional, Yang, Narae, additional, Shin, Seon M., additional, Jung, Hee, additional, Kim, Young J., additional, Jung, Woo S., additional, Park, Tae Y., additional, Suzuki, Kiyoshi, additional, Ito, Toshinori, additional, Uchida, Seiya, additional, Kamohara, Seika, additional, Ono, Naoya, additional, Takamura, Mitsuyuki, additional, Yokochi, Ayumu, additional, Maruyama, Kazuo, additional, Tapia, Patricio, additional, Thabaut, Katarzyna, additional, Thronicke, Anja, additional, Steele, Megan, additional, Matthes, Harald, additional, Herbstreit, Cornelia, additional, Schad, Friedemann, additional, Tian, Jiaxing, additional, Yang, Libo, additional, Tian, Tian, additional, Zhang, Hewei, additional, Tian, Xia, additional, Wang, CongCong, additional, Chai, Qian Yun, additional, Zhang, Lijuan, additional, Xia, Ruyu, additional, Huang, Na, additional, Fei, Yutong, additional, Liu, Jianpin, additional, Trent, Natalie, additional, Miraglia, Mindy, additional, Dusek, Jeffrey, additional, Pasalis, Edi, additional, Khalsa, Sat B., additional, Trifunovic-König, Milena, additional, Koch, Anna, additional, Uebelacker, Lisa, additional, Tremont, Geoffrey, additional, Gillette, Lee, additional, Epstein-Lubow, Gary, additional, Strong, David, additional, Abrantes, Ana, additional, Tyrka, Audrey, additional, Tran, Tanya, additional, Gaudiano, Brandon, additional, Miller, Ivan, additional, Ullmann, Gerhild, additional, Li, Yuhua, additional, Vaidya, Sujata, additional, Marathe, Vinod, additional, Vale, Ana C., additional, Motta, Jacquelyne, additional, Donadão, Fabíola, additional, Valente, Angela C., additional, Valente, Luana C. Carvalho, additional, Ghelman, Ricardo, additional, Vesovic, Dusan, additional, Jevdic, Dragan, additional, Jevdic, Aleksandar, additional, Jevdic, Katarina, additional, Djacic, Mihael, additional, Letic, Dragica, additional, Bozic, Drago, additional, Markovic, Marija, additional, Dunjic, Slobodan, additional, Ruscuklic, Gordana, additional, Baksa, Dezire, additional, Vrca, Kenan, additional, Vincent, Ann, additional, Wahner-Roedler, Dietlind, additional, Whipple, Mary, additional, Vogelius, Maria M., additional, Friesecke, Iris, additional, Gündling, Peter W., additional, Mahapatra, Saswati, additional, Hynes, Rebecca, additional, Van Rooy, Kimberly, additional, Looker, Sherry, additional, Ghosh, Aditya, additional, Bauer, Brent, additional, Cutshall, Susanne, additional, Walach, Harald, additional, Flores, Ana Borges, additional, Ofner, Michael, additional, Kastner, Andreas, additional, Schwarzl, Gerhard, additional, Schwameder, Hermann, additional, Alexander, Nathalie, additional, Strutzenberger, Gerda, additional, Bu, Xianwei, additional, Zhang, Jianping, additional, Wang, Shang, additional, Shi, Jinfeng, additional, Hao, Yu, additional, Wu, Jun, additional, Qiu, Zeji, additional, Wang, Yuh-Hai, additional, Lou, Chi-Jung, additional, Watts, Sam, additional, Wayne, Peter, additional, Vergara-Diaz, Gloria, additional, Gow, Brian, additional, Miranda, Jose, additional, Sudarsky, Lewis, additional, Macklin, Eric, additional, Wode, Kathrin, additional, Bergqvist, Jenny, additional, Bernhardsson, Britt-Marie, additional, Nordberg, Johanna Hök, additional, Sharp, Lena, additional, Henriksson, Roger, additional, Woo, Yeonju, additional, Hyun, Min K., additional, Wu, Hao, additional, Wang, Tian-Fang, additional, Zhao, Yan, additional, Wei, Yu, additional, Tian, Lei, additional, He, Lei, additional, Wang, Xue, additional, Wu, Ruohan, additional, Han, Mei, additional, Caldwell, Patrina H. Y., additional, Liu, Shigang, additional, Zhang, Jing, additional, Liu, Jianping, additional, Chai, Qianyun, additional, Guo, Zhongning, additional, Wang, Congcong, additional, Liu, Zhijun, additional, Li, Xun, additional, Yang, I. J., additional, Lincha, V. Ruberio, additional, Ahn, S. H., additional, Lee, D. U., additional, Shin, H. M., additional, Yang, Lu, additional, Yang, N., additional, Sung, H., additional, Shin, S. M., additional, Go, H. Y., additional, Jung, H., additional, Kim, Y., additional, Park, T. Y., additional, Yap, Angela, additional, Kwan, Yu H., additional, Tan, Chuen S., additional, Ibrahim, Syed, additional, Ang, Seng B., additional, Yayi, Alfred, additional, Yoo, Jeong E., additional, Yoo, Ho R., additional, Jang, Sae B., additional, Lee, Hye L., additional, Youssef, Ala’a, additional, Ezzat, Shahira, additional, Motaal, Amira Abdel, additional, El-Askary, Hesham, additional, Yu, Xiaotong, additional, Cui, Yashan, additional, Yun, Younghee, additional, Ahn, Jin-Hyang, additional, Jang, Bo-Hyung, additional, Kim, Kyu-Seok, additional, Choi, Inhwa, additional, Glinz, Augustina, additional, ten Brink, Fadime, additional, Büssing, Arnd, additional, Gutenbrunner, Christoph, additional, Helbrecht, Bert, additional, Fang, Tiesheng, additional, Meng, Fengxion, additional, Shen, Zhiming, additional, Zhang, Ruixin, additional, Wu, Fan, additional, Li, Ming, additional, Xuan, Xinyun, additional, Shen, Xueyong, additional, Ren, Ke, additional, Berman, Brian, additional, Zheng, Zian, additional, Wan, Yuxiang, additional, Ma, Xueyan, additional, Dong, Fei, additional, Zick, Suzie, additional, Harris, Richard, additional, Bae, Go E., additional, Kwon, Jung N., additional, Lee, Hye Y., additional, Nam, Jong K., additional, Lee, Sang D., additional, Lee, Dong H., additional, Han, Ji Y., additional, Yun, Young J., additional, Lee, Ji H., additional, Park, Hye L., additional, Park, Seong H., additional, Bocci, Chiara, additional, Ivaldi, Giovanni B., additional, Vietti, Ilaria, additional, Meaglia, Ilaria, additional, Guffi, Marta, additional, Ruggiero, Rubina, additional, Gualea, Marita, additional, Longa, Emanuela, additional, Bonucci, Massimo, additional, Croke, Sarah, additional, Rodriguez, Lourdes Diaz, additional, Caracuel-Martínez, Juan C., additional, Fajardo-Rodríguez, Manuel F., additional, Ariza-García, Angélica, additional, la Fuente, Francisca García-De, additional, Arroyo-Morales, Manuel, additional, Estrems, Maria S., additional, Gómez, Vicente G., additional, Sabater, Mónica Valero, additional, Ferreri, Rosaria, additional, Bernardini, Simonetta, additional, Pulcri, Roberto, additional, Cracolici, Franco, additional, Rinaldi, Massimo, additional, Porciani, Claudio, additional, Fisher, Peter, additional, Hughes, John, additional, Mendoza, Ariadna, additional, MacPherson, Hugh, additional, Filshie, Jacqueline, additional, Di Francesco, Antonia, additional, Bernardini, Alberto, additional, Messe, Monica, additional, Primitivo, Vincenzo, additional, Iasella, Piera A., additional, Taminato, Monica, additional, Alcantara, Jaqueline Do Carmo, additional, De Oliveira, Katia R., additional, Rodrigues, Debora C. De Azevedo, additional, Mumme, Juliana R. Campana, additional, Sunakozawa, Olga K. Matsumoto, additional, Filho, Vicente Odone, additional, Goldenberg, Joshua, additional, Day, Andrew, additional, Sasagawa, Masa, additional, Ward, Lesley, additional, Cooley, Kieran, additional, Gunnarsdottir, Thora, additional, Hjaltadottir, Ingibjorg, additional, Hajimonfarednejad, Mahdie, additional, Hannan, Nicole, additional, Hellsing, Rut, additional, Andermo, Susanne, additional, Arman, Maria, additional, von Hörsten, Iris, additional, Torrielo, Patricia Vásquez, additional, Vilaró, Carmen L. Andrade, additional, Cabrera, Francisco Cerda, additional, Hui, Henny, additional, Ziea, Eric, additional, Tsui, Dora, additional, Hsieh, Joyce, additional, Lam, Christine, additional, Chan, Edith, additional, Jensen, Mark P., additional, Battalio, Samuel L., additional, Chan, Joy, additional, Edwards, Karlyn A., additional, Gertz, Kevin J., additional, Day, Melissa A., additional, Sherlin, Leslie H., additional, Ehde, Dawn M., additional, Jang, Bo-Hyoung, additional, Börner, Anja, additional, Lee, Jihong, additional, Lee, Boram, additional, Chang, Gyu T., additional, Menassa, Alejandra, additional, Motoo, Yoshiharu, additional, Müller, Jürgen, additional, Rabini, Sabine, additional, Vinson, Bettina, additional, Storr, Martin, additional, Niemeijer, Martin, additional, Hoekman, Joop, additional, Ruijssenaaars, Wied, additional, Njoku, Faith C., additional, Norheim, Arne J., additional, Okumus, Filiz, additional, and Oncu-Celik, Halime, additional

Published
2017
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35. Gray Matter Volumes in Patients with Chronic Fatigue Syndrome

Author

Tang, Le-wei, Zheng, Hui, Chen, Liang, Zhou, Si-yuan, Huang, Wen-jing, Li, Ying, and Wu, Xi

Subjects
musculoskeletal diseases, Article Subject, virus diseases, human activities, nervous system diseases
Abstract

Chronic fatigue syndrome (CFS) is a debilitating and complex disorder characterized by profound fatigue with uncertain pathologic mechanism. Neuroimage may be an important key to unveil the central nervous system (CNS) mechanism in CFS. Although most of the studies found gray matter (GM) volumes reduced in some brain regions in CFS, there are many factors that could affect GM volumes in CFS, including chronic pain, stress, psychiatric disorder, physical activity, and insomnia, which may bias the results. In this paper, through reviewing recent literatures, we discussed these interferential factors, which overlap with the symptoms of CFS.

Published
2015
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36. Chinese herbal medicine TangBi Formula treatment of patients with type 2 diabetic distal symmetric polyneuropathy disease: study protocol for a randomized controlled trial.

Author

De Jin, Wen-jing Huang, Xiang Meng, Fan Yang, Yu-jiao Zheng, Qi Bao, Mei-zhen Zhang, Ya-nan Yang, Qing Ni, Feng-mei Lian, Xiao-lin Tong, Jin, De, Huang, Wen-Jing, Meng, Xiang, Yang, Fan, Zheng, Yu-Jiao, Bao, Qi, Zhang, Mei-Zhen, Yang, Ya-Nan, and Ni, Qing

Subjects
TYPE 2 diabetes diagnosis, TYPE 2 diabetes treatment, PATIENT compliance, CHINESE medicine, CLINICAL trials
Abstract

Background: Diabetic distal symmetric polyneuropathy (DSPN) is one of the most common microvascular complications of diabetes mellitus, and it has become a major public health problem worldwide because of its high and increasing prevalence, morbidity, and disability rate. The current medications for DSPN are not entirely satisfactory. Preliminary studies indicated that the Chinese herbal TangBi Formula may alleviate signs and symptoms and improve the velocity of nerve conduction in patients with DSPN. This study was designed to determine if Chinese herbal medicine used in combination with conventional treatment is more effective than conventional treatment alone.Methods/design: We are conducting a multicenter, placebo-controlled, double-blind, randomized, controlled clinical trial as a means of assessing the therapeutic effects of traditional Chinese medicine (TCM) treatment. A total of 188 patients will be randomized in a 1:1 ratio to a treatment group (TangBi Formula plus mecobalamin) and a control group (placebo plus mecobalamin). The test period lasts 6 months, during which all of the patients will be given standard medical care as recommended by established guidelines. The primary outcome will be development of differences in changes in clinical symptoms and signs in patients and changes in Michigan Diabetic Neuropathy Score (MDNS) between the two groups before and after treatment. The secondary outcome will be changes in nerve conduction velocity and in single clinical signs and symptoms. Safety assessments and adverse events will also be evaluated.Discussion: We postulate that patients with DSPN will benefit from therapy that includes TCM. If successful, this work will provide an evidence-based complementary therapeutic approach for treatment of DSPN.Trial Registration: ClinicalTrials.gov, NCT03010241 . Registered on 2 January 2017. [ABSTRACT FROM AUTHOR]

Published
2017
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38. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells

Author

Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishige, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Sheng, Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishige, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, and Li, Tao-Sheng

Abstract

Effects of antioxidants on the quality and genomic stability of induced pluripotent stem (iPS) cells were investigated with two human iPS cell lines (201B7 and 253G1). Cells used in this study were expanded from a single colony of each cell line with the addition of proprietary antioxidant supplement or homemade antioxidant cocktail in medium, and maintained in parallel for 2 months. The cells grew well in all culture conditions and kept "stemness". Although antioxidants modestly decreased the levels of intracellular reactive oxygen species, there were no differences in the expression of 53BP1 and pATM, two critical molecules related with DNA damage and repair, under various culture conditions. CGH analysis showed that the events of genetic aberrations were decreased only in the 253G1 iPS cells with the addition of homemade antioxidant cocktail. Long-term culture will be necessary to confirm whether low dose antioxidants improve the quality and genomic stability of iPS cells., Scientific reports, 4, 3779; 2014

Published
2014

39. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells

Author

Luo, Lan, primary, Kawakatsu, Miho, additional, Guo, Chao-Wan, additional, Urata, Yoshishige, additional, Huang, Wen-Jing, additional, Ali, Haytham, additional, Doi, Hanako, additional, Kitajima, Yuriko, additional, Tanaka, Takayuki, additional, Goto, Shinji, additional, Ono, Yusuke, additional, Xin, Hong-Bo, additional, Hamano, Kimikazu, additional, and Li, Tao-Sheng, additional

Published
2014
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40. Prim-O-glucosycimifugin attenuates liver injury in septic mice by inhibiting NLRP3 inflammasome/caspase-1 signaling cascades in macrophages.

Author

Liu, Lin-Ling, Yan, Xin, Xue, Kun-Yue, Wang, Xue-Mei, Li, Ling-Yu, Chen, Hong-Ying, Li, Ru-Li, Li, He, Lan, Jie, Xin, Juan-Juan, Li, Xue, Zhuo, Cai-Li, Wu, Zhuang, Zhang, Die, Huang, Wen-Jing, Wang, Ying-Ling, Li, Xin-Yue, Jiang, Wei, and Zhang, Heng-Yu

Abstract

Background: Liver dysfunction and liver failure are serious complications of sepsis, directly leading to septic progression and death. Now, there is no specific therapeutics available for sepsis-related liver dysfunction. Prim-O-glucosylcimifugin (POG), a chromone richest in the roots of Saposhnikovia divaricata (Turcz.) Schischk, is usually used to treat headache, rheumatoid arthritis and tetanus. While, the underlying mechanisms of POG against sepsis-induced liver damage and dysfunction are still not clear.Purpose: To study the anti-sepsis effect of POG, and its pharmacological mechanism to protect liver injury by weakening the function of macrophages in septic livers through inhibiting NOD-like receptor protein 3 (NLRP3) inflammasome pathway.Method: In vivo experiments, septic mouse model was induced by cecal ligation and puncture (CLP), and then the mortality was detected, liver inflammatory damages and plasma biomarkers of liver injury were evaluated by histopathological staining and biochemical assays, respectively. In vitro experiments, mouse primary peritoneal macrophages were treated with lipopolysaccharide (LPS) and ATP, and then the activated-inflammasomes, macrophage migration and polarization were detected by ASC immunofluorescence staining, transwell and flow cytometry assays, respectively. NLRP3 inflammasome components NLRP3, caspase-1, IL-1β and IL-18 protein expressions were detected using western blot assays, and the contents of IL-1β and IL-18 were measured by ELISA assays.Results: POG treatment significantly decreased the mortality, liver inflammatory damages, hepatocyte apoptosis and plasma biomarkers of liver injury in CLP-challenged male WT mice, which were comparable to those in ibuprofen (a putative anti-inflammatory drug)-supplemented septic male WT mice and septic NLRP3 deficient-male mice. POG supplementation significantly suppressed NLRP3 inflammasome activation in septic liver tissues and cultured macrophages, by significantly reducing NLRP3, cleaved-caspase-1, IL-1β and IL-18 levels, the activated-inflammasome ASC specks, and macrophage infiltration and migration, as well as M1-like polarization, but significantly increasing M2-like polarization. These findings were similar to the pharmacological effects of ibuprofen, NLRP3 deficiency, and a special NLRP3 inhibitor, MCC950.Conclusion: POG protected against sepsis by inhibiting NLRP3 inflammasome-mediated macrophage activation in septic liver and attenuating liver inflammatory injury, indicating that it may be a potential anti-sepsis drug candidate. [ABSTRACT FROM AUTHOR]

Published
2022
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41. N6-methyladenosine of TRIM27enhances the stem cell-type phenotype of cisplatin-resistant colorectal cancer cells

Author

Zheng, Jun-qiong, Zhan, Ying, Huang, Wen-jing, Chen, Zhi-yong, and Wu, Wei-hao

Abstract

Colorectal cancer (CRC), classified as a lethal form of cancer, substantially threatens human well-being. Cancer stem cells (CSCs) reflect subsets for cancerous cells having basic stem-cell type properties, being significantly involved in the development of chemoresistance and tumor relapsing. The aberrant TRIM27expression in various types of cancer indicates its potential involvement in cancer growth and progression. The current understanding of the TRIM27involvement in CRC remains limited. In current study indicated that TRIM27can potentially promote CSC-type phenotype of Cisplatin (DDP)-resistant CRC cells. YTHDF1recruitment onto m6A-amended TRIM27was crucial for facilitating the TRIM27translating process in DDP-resistant CRC cells. The present research proposes that TRIM27exhibits an oncogenic role by enhancing the CSC-type properties in DDP-resistant CRC viathe m6A-modified pathway. The potential therapy for combating the relapse of CRC may include TRIM27and YTHDF1, as they have been found to have significant roles in promoting CSC-type phenotypic characteristics.

Published
2023
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42. In-situ spectroscopic probe of the intrinsic structure feature of single-atom center in electrochemical CO/CO2 reduction to methanol.

Author

Ren, Xinyi, Zhao, Jian, Li, Xuning, Shao, Junming, Pan, Binbin, Salamé, Aude, Boutin, Etienne, Groizard, Thomas, Wang, Shifu, Ding, Jie, Zhang, Xiong, Huang, Wen-Yang, Zeng, Wen-Jing, Liu, Chengyu, Li, Yanguang, Hung, Sung-Fu, Huang, Yanqiang, Robert, Marc, and Liu, Bin

Subjects
ELECTROLYTIC reduction, ACTIVATION energy, ELECTRON density, X-ray absorption, INFRARED absorption, INFRARED spectroscopy, METHANOL
Abstract

While exploring the process of CO/CO2 electroreduction (COxRR) is of great significance to achieve carbon recycling, deciphering reaction mechanisms so as to further design catalytic systems able to overcome sluggish kinetics remains challenging. In this work, a model single-Co-atom catalyst with well-defined coordination structure is developed and employed as a platform to unravel the underlying reaction mechanism of COxRR. The as-prepared single-Co-atom catalyst exhibits a maximum methanol Faradaic efficiency as high as 65% at 30 mA/cm2 in a membrane electrode assembly electrolyzer, while on the contrary, the reduction pathway of CO2 to methanol is strongly decreased in CO2RR. In-situ X-ray absorption and Fourier-transform infrared spectroscopies point to a different adsorption configuration of *CO intermediate in CORR as compared to that in CO2RR, with a weaker stretching vibration of the C–O bond in the former case. Theoretical calculations further evidence the low energy barrier for the formation of a H-CoPc-CO– species, which is a critical factor in promoting the electrochemical reduction of CO to methanol. Deciphering the reaction mechanisms of CO/CO2 electroreduction to methanol remains challenging. Here the authors report the higher electron density of single-Co-atom center, along with a different adsorption configuration of *CO, is crucial for promoting the CO electroreduction to methanol. [ABSTRACT FROM AUTHOR]

Published
2023
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43. Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors.

Author

Jin, Jing-Hao, Zhou, Xue, Liu, Wang, Zhang, Zi-Hui, Huang, Shen-Xin, Zhao, Wen-Jing, and Chen, Xiao-Ren

Subjects
SIGNAL detection, NICOTIANA benthamiana, PEPTIDES, CHIMERIC proteins, PLANT-pathogen relationships, TOBACCO
Abstract

Objective: To examine the influence of widely used protein affinity tags and the tobacco PR1a signal peptide (SP) on detection, purification and bioactivity analyses of the small oomycete apoplastic effector SCR96 in planta. Results: Through agroinfiltration, the phytotoxic effector SCR96 of Phytophthora cactorum was expressed in Nicotiana benthamiana leaf apoplast as a fusion protein carrying single affinity tag (His, HA or FLAG) at either C- or N-terminus. Leaf necrosis caused by different affinity-tagged SCR96 varied among tags and replicates. All of tagged proteins can be detected by antibodies against SCR96. All of SCR96 fusions except N-terminally fused 6His-tagged protein were detected using tag antibodies, indicating that 6His tag may be degraded when fused at N-terminus. Interestingly, C-terminal His- and FLAG-tagged SCR96 maintained the biological activity after purification. In the substitution assay of SCR96 SP, we observed that PR1a SP can lead chimeric SCR96 expression in N. benthamiana, but the replacement totally disrupted its bioactivity. Conclusion: C-terminal His or FLAG tag, along with its original SP, is efficient enough to enable detection and purification of functional SCR96 from N. benthamiana leaf apoplast, which would facilitate plant–pathogen interaction studies. [ABSTRACT FROM AUTHOR]

Published
2023
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44. The metabolism of gelsevirine in human, pig, goat and rat liver microsomes.

Author

Zhang, Hua‐Hai, Yang, Wen‐Jia, Huang, Ya‐Jun, Li, Wen‐Jing, Zhang, Shuo‐Xin, and Liu, Zhao‐Ying

Subjects
LIVER microsomes, DRUG metabolism, METABOLISM, FLOWERING of plants, SWINE, RATS, GOATS
Abstract

Gelsemium is a small genus of flowering plants from the family Loganiaceae comprising five species, three of which, Gelsemium sempervirens (L.) J. St.‐Hil., G. elegans Benth and G. rankinii Small, are particularly popular. Compared with other alkaloids from G. elegans, gelsemine, gelsevirine and koumine exhibit equally potent anxiolytic effects and low toxicity. Although the pharmacological activities and metabolism of koumine and gelsemine have been reported in previous studies, the species differences of gelsevirine metabolism have not been well studied. In this study, the metabolism of gelsevirine was investigated by using liver microsomes of humans, pigs, goats and rats by means of HPLC‐QqTOF/MS. The results indicated that the metabolism of gelsevirine in liver microsomes had qualitative and quantitative species differences. Based on the results, the possible metabolic pathways of gelsevirine in liver microsomes were proposed. Investigation of the metabolism of gelsevirine will provide a basis for further studies of the in vivo metabolism of this drug. [ABSTRACT FROM AUTHOR]

Published
2021
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45. Social Company by a Receptive Mating Partner Facilitates Fear Extinction.

Author

Gao, Feng, Huang, Jie, Guan, Yan-Fei, Huang, Guo-Bin, Li, Wen-Jing, He, Xi-Yi, Qiu, Zi-Cong, Zhang, Yun-Long, Zhao, Shen-Ting, Li, Jianhua, Xuan, Aiguo, and Sun, Xiang-Dong

Subjects
BIOLOGICAL extinction, EXPOSURE therapy, POST-traumatic stress disorder, BEHAVIOR therapy, EXTINCTION (Psychology), SEXUAL partners
Abstract

Fear extinction remains an unresolved challenge for behavioral exposure therapy in patients with post-traumatic stress disorder (PTSD). Previous reports have suggested that social support from either familiar or unfamiliar same-sex partners is beneficial to attenuating fear responses during fear extinction and renewal. Despite that, few studies have examined the effects of social support in advance on fear extinction and/or retrieval. It is also not clear whether social company by a receptive mating partner in advance facilitates fear extinction. In the present study, we address these questions by introducing a co-housing method, where fear-conditioned male mice are co-housed with or without a receptive mating partner prior to fear extinction. We found that while co-housing with an ovariectomized female mouse showed little effect on fear extinction or retrieval, social company by a receptive mating partner in advance dramatically facilitates fear extinction. In addition, the number of cFos-positive neurons in the basolateral amygdala (BLA) were also found to be reduced in male mice accompanied with receptive mating partner in response to fear extinction and retrieval, indicating diminished neuronal activation. Electrophysiological studies further showed that the excitability of excitatory neurons in BLA was decreased, which is probably due to the attenuated basal level of excitatory synaptic transmission. Together, our observations demonstrate an effect of social company by a receptive mating partner can facilitate fear extinction and afford a possible cellular mechanism. [ABSTRACT FROM AUTHOR]

Published
2020
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47. Kir6.1 Knockdown Aggravates Cerebral Ischemia/Reperfusion-Induced Neural Injury in Mice.

Author

Dong, Yin‐Feng, Wang, Lin‐Xiao, Huang, Xu, Cao, Wen‐Jing, Lu, Ming, Ding, Jian‐Hua, Sun, Xiu‐Lan, and Hu, Gang

Subjects
CEREBRAL ischemia, REPERFUSION, NERVOUS system injuries, LABORATORY mice, ADENOSINE triphosphate, BRAIN diseases, STROKE
Abstract

Background and Purpose ATP-sensitive potassium (K- ATP) channels couple energy metabolism with electric activity, which play important roles in brain diseases including stroke. However, the impacts of Kir6.1-containing K- ATP channels that mainly expressed on glia in stroke remain unclear. Methods and Results In this study, we found that expression of Kir6.1 was significantly decreased in the ischemic brain area of C57 BL/6J mice after 1-h middle cerebral artery occlusion ( MCAO) and 24-h reperfusion. Then, we subjected Kir6.1 heterozygote knockout (Kir6.1+/−) mice to cerebral ischemia/reperfusion (I/R) injury and found that Kir6.1+/− mice exhibited exacerbated neurological disorder and enlarged infarct size, companied by glial over-activation and blood-brain barrier ( BBB) damages. Furthermore, we showed that Kir6.1 knockdown aggravated endoplasmic reticulum ( ER) stress and thereby increased the levels of proinflammatory factors tumor necrosis factor-α and interleukin-1β ( TNF-α and IL-1β) in mouse brain. Conclusions Our findings reveal that Kir6.1 knockdown exacerbates cerebral I/R-induced brain damages via increasing ER stress and inflammatory response, indicating that Kir6.1-containing K- ATP channels may be a potential therapeutic target for stroke. [ABSTRACT FROM AUTHOR]

Published
2013
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48. In Vivo Analysis of miR-34a Regulated Glucose Metabolism Related Genes in Megalobrama amblycephala.

Author

Miao, Ling-Hong, Lin, Yan, Huang, Xin, Pan, Wen-Jing, Zhou, Qun-Lan, Liu, Bo, Ren, Ming-Chun, Ge, Xian-Ping, and Pan, Liang-Kun

Subjects
GLUCOSE metabolism, FRESHWATER fishes, FEED utilization efficiency, GENE expression, PROTEIN kinases
Abstract

The Megalobrama amblycephala (M. amblycephala) is one of the most important economic freshwater fish in China. The molecular mechanism under the glucose intolerance responses which affects the growth performance and feed utilization is still confused. miR-34a was reported as a key regulator in the glucose metabolism, but how did the miR-34a exert its function in the metabolism of glucose/insulin in M. amblycephala was still unclear. In this study, we intraperitoneally injected the miR-34a inhibitor (80 nmol/100 g body weight) into M. amblycephala (fed with high starch diet, 45% starch) for 12 h, and then analyzed the gene expression profiling in livers by RNA-seq. The results showed that miR-34a expression in M. amblycephala livers was inhibited by injection of miR-34a inhibitor, and a total of 2212 differentially expressed genes (DEGs) were dysregulated (including 1183 up- and 1029 downregulated DEGs). Function enrichment analysis of DEGs showed that most of them were enriched in the peroxisome proliferator-activated receptor (PPAR), insulin, AMP-activated protein kinase (AMPK) and janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathways, which were all associated with the glucose/lipid metabolic and biosynthetic processes. In addition, we examined and verified the differential expression levels of some genes involved in AMPK signaling pathway by qRT-PCR. These results demonstrated that the inhibition of miR-34a might regulate glucose metabolism in M. amblycephala through downstream target genes. [ABSTRACT FROM AUTHOR]

Published
2018
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