1. Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells
- Author
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Vangipuram, SD, Yu, M, Tian, J, Stanhope, KL, Pasarica, M, Havel, PJ, Heydari, AR, and Dhurandhar, NV
- Subjects
Biomedical and Clinical Sciences ,Diabetes ,Nutrition ,Obesity ,2.1 Biological and endogenous factors ,Aetiology ,Metabolic and endocrine ,3T3-L1 Cells ,Acetyl-CoA Carboxylase ,Adenovirus Infections ,Human ,Adipocytes ,Adipogenesis ,Animals ,Cells ,Cultured ,Fatty Acid Synthases ,Gene Expression ,Glucose ,Humans ,Intra-Abdominal Fat ,Leptin ,Lipid Metabolism ,Male ,Mice ,RNA ,Messenger ,Rats ,Rats ,Wistar ,infectobesity ,adiposity ,Ad-36 ,glucose ,lactate ,3T3-L1 ,Medical and Health Sciences ,Education ,Endocrinology & Metabolism ,Biomedical and clinical sciences ,Health sciences - Abstract
ObjectiveHuman adenovirus Ad-36 causes adiposity in animal models and enhances differentiation and lipid accumulation in human and 3T3-L1 preadipocytes, which may, in part, explain the adipogenic effect of Ad-36. We determined the consequences of Ad-36 infection on leptin and glucose metabolism in fat cells.Design3T3-L1 preadipocytes were used to determine the effect of infection by human adenoviruses Ad-36, Ad-2, Ad-9 and Ad-37 on leptin secretion and lipid accumulation. Rat primary adipocytes were used to determine the effect of Ad-36 infection on leptin secretion and glucose uptake in vitro. Furthermore, the effect of Ad-36 on expressions of leptin and selected genes of de novo lipogenesis pathway of visceral adipose tissue were compared ex vivo, between Ad-36 infected and uninfected control rats.ResultsAd-36 suppressed the expression of leptin mRNA in 3T3-L1 cells by approximately 58 and 52% on days 3 and 5 post-infection, respectively. Leptin release normalized to cellular lipid content was 51% lower (P
- Published
- 2007