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2. Effect of Silicon Nitride Coating on Titanium Surface: Biocompatibility and Antibacterial Properties.

Author

Tani, Akina, Tsubouchi, Harumitsu, Ma, Lin, Taniguchi, Yurie, Kobayashi, Yasuyuki, Nakai, Mariko, Komasa, Satoshi, and Hashimoto, Yoshiya

Subjects
BONE marrow cells, TISSUE differentiation, SILICON surfaces, OLDER patients, METALLIC surfaces, CELL adhesion, SILICON nitride
Abstract

In recent years, with the advent of a super-aged society, lifelong dental care has gained increasing emphasis, and implant therapy for patients with an edentulous jaw has become a significant option. However, for implant therapy to be suitable for elderly patients with reduced regenerative and immunological capabilities, higher osteoconductive and antimicrobial properties are required on the implant surfaces. Silicon nitride, a non-oxide ceramic known for its excellent mechanical properties and biocompatibility, has demonstrated high potential for inducing hard tissue differentiation and exhibiting antibacterial properties. In this study, silicon nitride was deposited on pure titanium metal surfaces and evaluated for its biocompatibility and antibacterial properties. The findings indicate that silicon nitride improves the hydrophilicity of the material surface, enhancing the initial adhesion of rat bone marrow cells and promoting hard tissue differentiation. Additionally, the antibacterial properties were assessed using Staphylococcus aureus, revealing that the silicon nitride-coated surfaces exhibited significant antibacterial activity. Importantly, no cytotoxicity was observed, suggesting that silicon nitride-coated titanium could serve as a novel implant material. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Effect of Er:YAG Pulsed Laser-Deposited Hydroxyapatite Film on Titanium Implants on M2 Macrophage Polarization In Vitro and Osteogenesis In Vivo.

Author

Ma, Lin, Li, Min, Komasa, Satoshi, Hontsu, Shigeki, Hashimoto, Yoshiya, Okazaki, Joji, and Maekawa, Kenji

Subjects
ERBIUM, BONE growth, YTTRIUM aluminum garnet, MACROPHAGES, OSTEOINDUCTION, MESENCHYMAL stem cells, TITANIUM, BONE marrow
Abstract

In a previous study, we successfully coated hydroxyapatite (HAp) onto titanium (Ti) plates using the erbium-doped yttrium aluminum garnet pulsed-laser deposition (Er:YAG-PLD) method. In this study, we performed further experiments to validate the in vitro osteogenic properties, macrophage polarization, and in vivo osseointegration activity of HAp-coated Ti (HAp-Ti) plates and screws. Briefly, we coated a HAp film onto the surfaces of Ti plates and screws via Er:YAG-PLD. The surface morphological, elemental, and crystallographic analyses confirmed the successful surface coating. The macrophage polarization and osteogenic induction were evaluated in macrophages and rat bone marrow mesenchymal stem cells, and the in vivo osteogenic properties were studied. The results showed that needle-shaped nano-HAp promoted the early expression of osteogenic and immunogenic genes in the macrophages and induced excellent M2 polarization properties. The calcium deposition and osteocalcin production were significantly higher in the HAp-Ti than in the uncoated Ti. The implantation into rat femurs revealed that the HAp-coated materials had superior osteoinductive and osseointegration activities compared with the Ti, as assessed by microcomputed tomography and histology. Thus, HAp film on sandblasted Ti plates and screws via Er:YAG-PLD enhances hard-tissue differentiation, macrophage polarization, and new bone formation in tissues surrounding implants both in vitro and in vivo. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Characterization of Hydroxyapatite Film Obtained by Er:YAG Pulsed Laser Deposition on Sandblasted Titanium: An In Vitro Study

Author

Ma, Lin, primary, Li, Min, additional, Komasa, Satoshi, additional, Yan, Sifan, additional, Yang, Yuanyuan, additional, Nishizaki, Mariko, additional, Chen, Liji, additional, Zeng, Yuhao, additional, Wang, Xin, additional, Yamamoto, Ei, additional, Hontsu, Shigeki, additional, Hashimoto, Yoshiya, additional, and Okazaki, Joji, additional

Published
2022
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21. Effect of Argon-Based Atmospheric Pressure Plasma Treatment on Hard Tissue Formation on Titanium Surface

Author

Komasa, Satoshi, primary, Kusumoto, Tetsuji, additional, Hayashi, Rina, additional, Takao, Seiji, additional, Li, Min, additional, Yan, Sifan, additional, Zeng, Yuhao, additional, Yang, Yuanyuan, additional, Hu, Hui, additional, Kobayashi, Yasuyuki, additional, Agariguchi, Akinori, additional, Nishida, Hisataka, additional, Hashimoto, Yoshiya, additional, and Okazaki, Joji, additional

Published
2021
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22. Respiratory FimA-Specific Secretory IgA Antibodies Upregulated by DC-Targeting Nasal Double DNA Adjuvant Are Essential for Elimination of Porphyromonas gingivalis

Author

Kataoka, Kosuke, Kawabata, Shigetada, Koyanagi, Kayo, Hashimoto, Yoshiya, Miyake, Tatsuro, Fujihashi, Kohtaro, Kataoka, Kosuke, Kawabata, Shigetada, Koyanagi, Kayo, Hashimoto, Yoshiya, Miyake, Tatsuro, and Fujihashi, Kohtaro

Abstract

Our previous studies showed that a combination of a DNA plasmid encoding Flt3 ligand (pFL) and CpG oligodeoxynucleotides 1826 (CpG ODN) (FL/CpG) as a nasal adjuvant provoked antigen-specific immune responses. In this study, we investigated the efficacy of a nasal vaccine consisting of FimA as the structural subunit of Porphyromonas gingivalis (P. gingivalis) fimbriae and FL/CpG for the induction of FimA-specific antibody (Ab) responses and their protective roles against nasal and lung infection by P. gingivalis, a keystone pathogen in the etiology of periodontal disease. C57BL/6 mice were nasally immunized with recombinant FimA (rFimA) plus FL/CpG three times at weekly intervals. As a control, mice were given nasal rFimA alone. Nasal washes (NWs) and bronchoalveolar lavage fluid (BALF) of mice given nasal rFimA plus FL/CpG resulted in increased levels of rFimA-specific secretory IgA (SIgA) and IgG Ab responses when compared with those in controls. Significantly increased numbers of CD8- or CD11b-expressing mature-type dendritic cells (DCs) were detected in the respiratory inductive and effector tissues of mice given rFimA plus FL/CpG. Additionally, significantly upregulated Th1/Th2-type cytokine responses by rFimA-stimulated CD4+ T cells were noted in the respiratory effector tissues. When mice were challenged with live P. gingivalis via the nasal route, mice immunized nasally with rFimA plus FL/CpG inhibited P. gingivalis colonization in the nasal cavities and lungs. In contrast, controls failed to show protection. Of interest, when IgA-deficient mice given nasal rFimA plus FL/CpG were challenged with nasal P. gingivalis, the inhibition of bacterial colonization in the respiratory tracts was not seen. Taken together, these results show that nasal FL/CpG effectively enhanced DCs and provided balanced Th1- and Th2-type cytokine response-mediated rFimA-specific IgA protective immunity in the respiratory tract against P. gingivalis. A nasal administration with rFimA an

Published
2021

27. Augmentation of Bone Regeneration by Depletion of Stress-Induced Senescent Cells Using Catechin and Senolytics

Author

Honda, Yoshitomo, primary, Huang, Anqi, additional, Tanaka, Tomonari, additional, Han, Xiaoyu, additional, Gao, Beiyuan, additional, Liu, Haitao, additional, Wang, Xinchen, additional, Zhao, Jianxin, additional, Hashimoto, Yoshiya, additional, Yamamoto, Kazuyo, additional, Matsumoto, Naoyuki, additional, Baba, Shunsuke, additional, and Umeda, Makoto, additional

Published
2020
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31. Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Author

Honda, Yoshitomo, primary, Takeda, Yoshihiro, additional, Li, Peiqi, additional, Huang, Anqi, additional, Sasayama, Satoshi, additional, Hara, Eiki, additional, Uemura, Naoya, additional, Ueda, Mamoru, additional, Hashimoto, Masanori, additional, Arita, Kenji, additional, Matsumoto, Naoyuki, additional, Hashimoto, Yoshiya, additional, Baba, Shunsuke, additional, and Tanaka, Tomonari, additional

Published
2018
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33. Micro-patterning technique for capillary blood vessels on Polytetrafluoroenthylene substrate

Author

Kusunoki, Masanobu, Wada, Takuto, Kanda, Hirotaka, Togo, Hidetaka, Hayami, Takashi, Hashimoto, Yoshiya, Nishikawa, Hiroaki, and Hontsu, Shigeki

Subjects
cardiovascular system
Abstract

A pattering technique for blood vessels on biochips is discussed herein. Polytetrafluoroethylene (PTFE) acts as an inhibitor of cell adhesion. To produce a biocompatible region, we performed Ar plasma irradiation of the PTFE substrate. F is removed from C-F bonds in the _n structures, and amorphous C is apparently brought to the surface of PTFE.. The strong hydrophobic properties of PTFE are thus altered to hydrophilic properties by plasma irradiation. Conventional photolithography is then used to produce a blood vessel-like network scaffold. After l-week cell culture of human umbilical vein endothelial cells (HUVEC), the blood vessel pattem can be observed clearly on fluorescence microscopy., Departmental Bulletin Paper

Published
2010

34. Human Gingival Integration-Free iPSCs

Author

Umezaki, Yasuyuki, Hashimoto, Yoshiya, Nishishita, Naoki, Kawamata, Shin, and Baba, Shunsuke

Subjects
episomal vectors, pluripotent, plasmid, gingival fibroblasts, mesenchymal, human gingival tissues
Abstract

Mesenchymal stem cells (MSCs) are considered a potential autologous therapy for tissue engineering. The available procedures for MSC retrieval from patients are invasive, and their limited in vitro proliferation restricts their use in the treatment of damaged tissues. Therefore, it is important to establish an alternative and safe source of MSCs. The objective of this study was to demonstrate induced pluripotent stem cell (iPSC) generation from a combination of an accessible source tissue and an integration-free method, we also attempted the differentiation of iPSCs into MSC-like cells (MSLCs) for future autologous tissue engineering. iPSCs were derived from human gingival tissues, which are easily accessible in the field of dentistry, via the use of non-integrating episomal plasmids. Established iPSCs expressed embryonic stem (ES) cell-specific markers, as assessed by gene analysis and immunocytochemistry. Embryoid bodies and teratoma formation were formed from iPSCs, showing their capacity to differentiate into three germ layers. Furthermore, we were successful in differentiating iPSCs into MSLCs. They tested positively for their capacity of trilineage differentiation. Our results demonstrate that human gingival integration-free iPSCs, readily accessible stem cells generated using episomal plasmid vectors, are a promising source of MSLCs, which can be used in tissue regeneration.

Published
2015
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39. Local Controlled Release of Polyphenol Conjugated with Gelatin Facilitates Bone Formation

Author

Honda, Yoshitomo, primary, Tanaka, Tomonari, additional, Tokuda, Tomoko, additional, Kashiwagi, Takahiro, additional, Kaida, Koji, additional, Hieda, Ayato, additional, Umezaki, Yasuyuki, additional, Hashimoto, Yoshiya, additional, Imai, Koichi, additional, Matsumoto, Naoyuki, additional, Baba, Shunsuke, additional, and Shimizutani, Kimishige, additional

Published
2015
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41. Effectiveness of scaffolds with pre-seeded mesenchymal stem cells in bone regeneration -Assessment of osteogenic ability of scaffolds implanted under the periosteum of the cranial bone of rats

Author

Baba, Shunsuke, Inoue, Takeomi, Hashimoto, Yoshiya, Kimura, Daisuke, Ueda, Masatoshi, Sakai, Kana, Matsumoto, Naoyuki, Hiwa, Chiaki, Adachi, Taiji, Hojo, Masaki, Baba, Shunsuke, Inoue, Takeomi, Hashimoto, Yoshiya, Kimura, Daisuke, Ueda, Masatoshi, Sakai, Kana, Matsumoto, Naoyuki, Hiwa, Chiaki, Adachi, Taiji, and Hojo, Masaki

Published
2010

42. Effectiveness of scaffolds with pre-seeded mesenchymal stem cells in bone regeneration -Assessment of osteogenic ability of scaffolds implanted under the periosteum of the cranial bone of rats

Author

40243323, 70252492, Baba, Shunsuke, Inoue, Takeomi, Hashimoto, Yoshiya, Kimura, Daisuke, Ueda, Masatoshi, Sakai, Kana, Matsumoto, Naoyuki, Hiwa, Chiaki, Adachi, Taiji, Hojo, Masaki, 40243323, 70252492, Baba, Shunsuke, Inoue, Takeomi, Hashimoto, Yoshiya, Kimura, Daisuke, Ueda, Masatoshi, Sakai, Kana, Matsumoto, Naoyuki, Hiwa, Chiaki, Adachi, Taiji, and Hojo, Masaki

Abstract

To date, there has been no study on the development of novel regimens based on the following tissue engineering principles: seeding and culturing mesenchymal stem cells (MSCs) on a scaffold before surgery or injecting cultured MSCs into a scaffold during surgery. The purpose of this study was to assess the in vivo osteogenic ability of scaffold/MSCs implanted beneath the periosteum of the cranial bone of rats in three different sample groups: one in which MSCs were pre-seeded and cultured on a scaffold to produce the 3-D woven fabric scaffold/MSC composite using osteo-lineage induction medium, one in which cultured MSCs produced by osteolineage induction in cell cultivation flasks were injected into a scaffold during surgery and a control group, in which only the 3-D woven fabric scaffold was implanted. The results indicate that pre-seeding MSCs on a scaffold leads to a higher osteogenic ability than injecting cultured MSCs into a scaffold during surgery.

Published
2010

45. Releasing Behavior of Lipopolysaccharide from Gelatin Modulates Inflammation, Cellular Senescence, and Bone Formation in Critical-Sized Bone Defects in Rat Calvaria.

Author

Zhao, Jianxin, Honda, Yoshitomo, Tanaka, Tomonari, Hashimoto, Yoshiya, and Matsumoto, Naoyuki

Subjects
BONE growth, GELATIN, CELLULAR aging, CALVARIA, P16 gene, BONE regeneration, X-ray imaging, SOFT X rays
Abstract

Lipopolysaccharide (LPS) is a well-known strong inducer of inflammation. However, there is little information regarding how LPS-release behavior affects cellular senescence at the affected area. In this paper, we demonstrate that a vacuum-heating technique (dehydrothermal treatment) can be utilized to prepare an LPS sustained-release gelatin sponge (LS-G). LPS sustained release from gelatin leads to the long-term existence of senescent cells in critical-sized bone defects in rat calvaria. Three types of gelatin sponges were prepared in this study: a medical-grade gelatin sponge with extremely low LPS levels (MG), LS-G, and a LPS rapid-release gelatin sponge (LR-G). Histological (H-E) and immunohistochemical (COX-2, p16, and p21) staining were utilized to evaluate inflammatory reactions and cellular senescence one to three weeks after surgery. Soft X-ray imaging was utilized to estimate new bone formation in the defects. The LR-G led to stronger swelling and COX-2 expression in defects compared to the MG and LS-G at 1 week. Despite a small inflammatory reaction, LS-G implantation led to the long-term existence of senescent cells and hampered bone formation compared to the MG and LR-G. These results suggest that vacuum heating is a viable technique for preparing different types of materials for releasing bacterial components, which is helpful for developing disease models for elucidating cellular senescence and bone regeneration. [ABSTRACT FROM AUTHOR]

Published
2020
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