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14. Mechanism of Mitochondrial Kinetic Imbalance and Nrf2 Signaling Pathway-Mediated Oxidative Stress in Nickel and/or Chromium-Induced Kidney Injury in Mice.

Author

Du, Jun, Li, Zhengqing, Cao, Xianhong, Qi, Qiurong, Wang, Luqi, Liu, Ping, Chen, Yifei, Hu, Guoliang, Guo, Xiaoquan, and Gao, Xiaona

Subjects
MITOCHONDRIAL dynamics, WEIGHT gain, KIDNEY physiology, HEAVY metals, CHROMIUM
Abstract

Nickel and chromium are both common heavy metals that pose serious environmental and health hazards. However, the exact mechanism by which nickel and/or chromium cause renal injury is unclear. Therefore, we explored the molecular mechanisms of renal injury caused by nickel and/or chromium poisoning from the perspective of mitochondrial dynamics and the Nrf2 antioxidant pathway. In this study, eighty 6-week-old C57BL/6J mice were randomly divided into four groups: control (Con, untreated), nickel (Ni, 110 mg/L Ni2+), chromium (Cr, 50 mg/L Cr6+), and combined nickel–chromium (Ni + Cr, 110 mg/L Ni2+, 50 mg/L Cr6+). The results showed that chronic nickel and/or chromium exposure inhibited body weight gain and impaired kidney function and structure in mice. Chronic nickel and/or chromium exposure led to the disruption of mitochondrial dynamics and thus induced oxidative stress. On the other hand, the Nrf2 antioxidant pathway may play an important regulatory role in mitigating oxidative stress-induced oxidative damage in kidney. The present study partially elucidated the molecular mechanism of renal injury induced by nickel and/or chromium exposure in mice and the regulatory role of the Nrf2 pathway in inducing oxidative injury from the perspective of mitochondrial dynamics. This provides a theoretical basis for the development of prevention and control strategies, and environmental protection measures. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Wogonin Inhibits Apoptosis and Necroptosis Induced by Nephropathogenic Infectious Bronchitis Virus in Chicken Renal Tubular Epithelial Cells.

Author

Qi, Qiurong, Li, Ying, Ding, Mengbing, Huang, Cheng, Omar, Salma Mbarouk, Shi, Yan, Liu, Ping, Cai, Gaofeng, Zheng, Zhanhong, Guo, Xiaoquan, and Gao, Xiaona

Subjects
AVIAN infectious bronchitis virus, EPITHELIAL cells, MEMBRANE potential, MITOCHONDRIAL membranes, POULTRY industry, APOPTIN
Abstract

NIBV is an acute and highly contagious virus that has a major impact on the poultry industry. Wogonin, as a flavonoid drug, has antiviral effects, but there have been no reports indicating its role in renal injury caused by NIBV infection. The aim of this study is to investigate the antiviral effect of wogonin against NIBV. Renal tubular epithelial cells were isolated and cultured, and divided into four groups: Con, Con+Wog, NIBV and NIBV+Wog. We found that wogonin significantly inhibited the copy number of NIBV and significantly alleviated NIBV-induced cell apoptosis and necrosis. Moreover, wogonin inhibited the reduction in mitochondrial membrane potential and the aberrant opening of mPTP caused by NIBV. In conclusion, wogonin can protect renal tubular epithelial cells from damage by inhibiting the replication of NIBV and preventing mitochondrial apoptosis and necroptosis induced by NIBV. [ABSTRACT FROM AUTHOR]

Published
2024
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19. The 16S rDNA high-throughput sequencing correlation analysis of milk and gut microbial communities in mastitis Holstein cows

Author

Jiang, Chenxi, primary, Hou, Xiaolu, additional, Gao, Xiaona, additional, Liu, Pei, additional, Guo, Xiaoquan, additional, Hu, Guoliang, additional, Li, Qingqing, additional, Huang, Cheng, additional, Li, Guyue, additional, Fang, Weile, additional, Mai, Wanrui, additional, Wu, Cong, additional, Xu, Zheng, additional, and Liu, Ping, additional

Published
2023
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23. PRRSV Elimination in a Farrow-to-Finish Pig Herd Using Herd Closure and Rollover Approach

Author

Hu, Ruiming, primary, Zhang, Tiansheng, additional, Lai, Rongbin, additional, Ding, Zhen, additional, Zhuang, Yu, additional, Liu, Hao, additional, Cao, Huabin, additional, Gao, Xiaona, additional, Luo, Junrong, additional, Chen, Zheng, additional, Zhang, Caiying, additional, Liu, Ping, additional, Guo, Xiaoquan, additional, Hu, Guoliang, additional, Ding, Nengshui, additional, and Deng, Shunzhou, additional

Published
2023
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32. Preparation of the RIPK3 Polyclonal Antibody and Its Application in Immunoassays of Nephropathogenic Infectious Bronchitis Virus-Infected Chickens

Author

Tian, Guanming, primary, Shi, Yan, additional, Cao, Xianhong, additional, Chen, Wei, additional, Gu, Yueming, additional, Li, Ning, additional, Huang, Cheng, additional, Zhuang, Yu, additional, Li, Guyue, additional, Liu, Ping, additional, Hu, Guoliang, additional, Gao, Xiaona, additional, and Guo, Xiaoquan, additional

Published
2022
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33. Baicalin Attenuates H 2 O 2 -Induced Oxidative Stress by Regulating the AMPK/Nrf2 Signaling Pathway in IPEC-J2 Cells.

Author

Liang, Jiahua, Zhou, Ying, Cheng, Xinyi, Chen, Jiaqi, Cao, Huabin, Guo, Xiaoquan, Zhang, Caiying, Zhuang, Yu, and Hu, Guoliang

Subjects
OXIDATIVE stress, CELLULAR signal transduction, AMP-activated protein kinases, GENE expression, OXIDANT status
Abstract

Oxidative stress can adversely affect the health status of the body, more specifically by causing intestinal damage by disrupting the permeability of the intestinal barrier. This is closely related to intestinal epithelial cell apoptosis caused by the mass production of reactive oxygen species (ROS). Baicalin (Bai) is a major active ingredient in Chinese traditional herbal medicine that has antioxidant, anti-inflammatory, and anti-cancer properties. The purpose of this study was to explore the underlying mechanisms by which Bai protects against hydrogen peroxide (H2O2)-induced intestinal injury in vitro. Our results indicated that H2O2 treatment caused injury to IPEC-J2 cells, resulting in their apoptosis. However, Bai treatment attenuated H2O2-induced IPEC-J2 cell damage by up-regulating the mRNA and protein expression of ZO-1, Occludin, and Claudin1. Besides, Bai treatment prevented H2O2-induced ROS and MDA production and increased the activities of antioxidant enzymes (SOD, CAT, and GSH-PX). Moreover, Bai treatment also attenuated H2O2-induced apoptosis in IPEC-J2 cells by down-regulating the mRNA expression of Caspase-3 and Caspase-9 and up-regulating the mRNA expression of FAS and Bax, which are involved in the inhibition of mitochondrial pathways. The expression of Nrf2 increased after treatment with H2O2, and Bai can alleviate this phenomenon. Meanwhile, Bai down-regulated the ratio of phosphorylated AMPK to unphosphorylated AMPK, which is indicative of the mRNA abundance of antioxidant-related genes. In addition, knockdown of AMPK by short-hairpin RNA (shRNA) significantly reduced the protein levels of AMPK and Nrf2, increased the percentage of apoptotic cells, and abrogated Bai-mediated protection against oxidative stress. Collectively, our results indicated that Bai attenuated H2O2-induced cell injury and apoptosis in IPEC-J2 cells through improving the antioxidant capacity through the inhibition of the oxidative stress-mediated AMPK/Nrf2 signaling pathway. [ABSTRACT FROM AUTHOR]

Published
2023
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37. Luteolin Attenuates APEC-Induced Oxidative Stress and Inflammation via Inhibiting the HMGB1/TLR4/NF-κB Signal Axis in the Ileum of Chicks.

Author

Cao, Zhanyou, Xing, Chenghong, Cheng, Xinyi, Luo, Junrong, Hu, Ruiming, Cao, Huabin, Guo, Xiaoquan, Yang, Fan, Zhuang, Yu, and Hu, Guoliang

Subjects
LUTEOLIN, OXIDATIVE stress, CHICKS, ESCHERICHIA coli, FLAVONES, ILEUM, POULTRY farms
Abstract

Simple Summary: Avian colibacillosis is one of the major causes of animal death and egg-production decline, which has caused economic losses to the poultry industry. When encountering avian colibacillosis, many poultry farms tend to use antibiotics to reduce losses. Additionally, the utilization of antimicrobials is also disputable. Our study shows that luteolin can alleviate the inflammation and oxidative stress caused by Escherichia coli in the ilea of chicks and may be used as a substitute for antibiotics to control avian colibacillosis. Avian pathogenic E. coli (APEC) is typically the cause of avian colibacillosis, which can result in oxidative stress, inflammation, and intestinal damage (APEC). Luteolin, in the form of glycosylation flavone, has potent anti-inflammatory and anti-oxidative properties. However, its effects on APEC-induced intestinal oxidative stress and NF-κB-mediated inflammation in chicks remains poorly understood. After hatching, one-day-old chicks were stochastically assigned to four groups: a control group (basic diet), an E. coli group (basic diet) and L10 and L20 groups (with a dry matter of luteolin diet 10 mg/kg and 20 mg/kg, respectively), with fifteen chicks in each group and one repeat per group. They were pretreated for thirteen days. The body weight, mortality, histopathological changes in the ileum, antioxidant status, and the mRNA and protein-expression levels of factors associated with the HMGB1/TLR4/NF-κB signal axis of the chicks were measured. The results showed that luteolin treatment decreased the mRNA and protein-expression level of the related factors of HMGB1/TLR4/NF-κB signal axis in the ileum, reduced inflammation, increased antioxidant enzyme activity, and reduced intestinal injury. Collectively, luteolin alleviated APEC-induced intestinal damage by means of hindering the HMGB1/TLR4/NF-κB signal axis, which suggests that luteolin could be a good method for the prevention and treatment of avian colibacillosis. [ABSTRACT FROM AUTHOR]

Published
2023
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39. Investigation of the Crosstalk between GRP78/PERK/ATF-4 Signaling Pathway and Renal Apoptosis Induced by Nephropathogenic Infectious Bronchitis Virus Infection

Author

Chen, Wei, primary, Huang, Cheng, additional, Shi, Yan, additional, Li, Ning, additional, Wang, Enqi, additional, Hu, Ruiming, additional, Li, Guyue, additional, Yang, Fan, additional, Zhuang, Yu, additional, Liu, Ping, additional, Hu, Guoliang, additional, Gao, Xiaona, additional, and Guo, Xiaoquan, additional

Published
2022
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48. Characterization of Escherichia colipathogenicity and drug resistance in yolk peritonitis

Author

Li, Qingqing, Fang, Weile, Chen, Shupeng, Li, Guyue, Jiang, Chenxi, Zhuang, Yu, Li, Lin, Liu, Pei, Guo, Xiaoquan, Hu, Guoliang, Liu, Ping, and Gao, Xiaona

Abstract

Yolk Peritonitis can lead to a rapid decline in egg production, which seriously affects the health of laying hens and the profitability of chicken farms. Escherichia coli(E. coli) is the most common cause of yolk peritonitis in laying hens. In this study, bacterial samples were collected from the ovaries and fallopian tubes of laying hens with suspected yolk peritonitis from a laying farm in Jiangsu Province, and their pathogenicity and drug resistance were investigated. Initially, morphological and biochemical detection methods were employed to isolate and identify the pathogenic bacteria. The results showed that a total of 16 strains of E. coliwere isolated from laying hens with yolk peritonitis. Subsequently, the drug resistance and pathogenicity of a randomly selected E. colistrain were analyzed and predicted by genome sequencing technology, and the drug resistance of E. coli was verified by drug sensitivity test and PCR. Finally, the virulence was verified by infection experiment in mice. The study revealed that the egg-yolk peritonitis in laying hens was caused by E. coliinfection, and the genome sequencing analysis revealed that the bacteria had multidrug resistance and high virulence. The drug susceptibility testing indicates that E. coliexhibited resistance to aminoglycosides, β-lactam, macrolides, fluoroquinolones, and sulfonamides. In this study, resistance genes including KdpE, aadA5, APH(3 ")-ID, APH(6)-ID, and TEM-1 were identified, and their expression levels varied across different stages of bacterial growth. The results of virulence analysis indicated a mortality rate of 50% in mice infected with E. coliat a concentration of 2.985 × 107CFU/mL. E. coliinfection resulted in damage to various tissues and organs in mice, with the intestinal tissue structure being the most severely affected. This study provides a reference for the study of drug resistance mechanisms in E. coliand provides valuable insights into the selection of drugs for the treatment of vitelline peritonitis.

Published
2024
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