210 results on '"Fallacara A"'
Search Results
2. Proof of concept for quantitative adverse outcome pathway modeling of chronic toxicity in repeated exposure
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Ito, Shigeaki, Mukherjee, Sayak, Erami, Kazuo, Muratani, Shugo, Mori, Akina, Ichikawa, Sakuya, White, William, Yoshino, Kei, and Fallacara, Dawn
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- 2024
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3. Proof of concept for quantitative adverse outcome pathway modeling of chronic toxicity in repeated exposure
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Shigeaki Ito, Sayak Mukherjee, Kazuo Erami, Shugo Muratani, Akina Mori, Sakuya Ichikawa, William White, Kei Yoshino, and Dawn Fallacara
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Medicine ,Science - Abstract
Abstract Adverse Outcome Pathway (AOP) is a useful tool to glean mode of action (MOE) of a chemical. However, in order to use it for the purpose of risk assessment, an AOP needs to be quantified using in vitro or in vivo data. Majority of quantitative AOPs developed so far, were for single exposure to progressively higher doses. Limited attempts were made to include time in the modeling. Here as a proof-of concept, we developed a hypothetical AOP, and quantified it using a virtual dataset for six repeated exposures using a Bayesian Network Analysis (BN) framework. The virtual data was generated using realistic assumptions. Effects of each exposure were analyzed separately using a static BN model and analyzed in combination using a dynamic BN (DBN) model. Our work shows that the DBN model can be used to calculate the probability of adverse outcome when other upstream KEs were observed earlier. These probabilities can help in identification of early indicators of AO. In addition, we also developed a data driven AOP pruning technique using a lasso-based subset selection, and show that the causal structure of AOP is itself dynamic and changes over time. This proof-of-concept study revealed the possibility for expanding the applicability of the AOP framework to incorporate biological dynamism in toxicity appearance by repeated insults.
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- 2024
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4. The challenge of contemporary architecture didactics: Designing in dialogue with Artificial Intelligence.
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Fallacara, Giuseppe, Costantino, Dario, and Cavaliere, Ilaria
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- 2024
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5. Herman-Kluk propagator is free from zero-point energy leakage
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Buchholz, Max, Fallacara, Erika, Gottwald, Fabian, Ceotto, Michele, Grossmann, Frank, and Ivanov, Sergei D.
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Physics - Chemical Physics - Abstract
Semiclassical techniques constitute a promising route to approximate quantum dynamics based on classical trajectories starting from a quantum-mechanically correct distribution. One of their main drawbacks is the so-called zero-point energy (ZPE) leakage, that is artificial redistribution of energy from the modes with high frequency and thus high ZPE to that with low frequency and ZPE due to classical equipartition. Here, we show that an elaborate semiclassical formalism based on the Herman-Kluk propagator is free from the ZPE leakage despite utilizing purely classical propagation. This finding opens the road to correct dynamical simulations of systems with a multitude of degrees of freedom that cannot be treated fully quantum-mechanically due to the exponential increase of the numerical effort., Comment: 6 pages 2 figures
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- 2018
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6. Perfluoroalkyl substances exposure and immunity, allergic response, infection, and asthma in children: review of epidemiologic studies
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von Holst, Haley, Nayak, Pratibha, Dembek, Zygmunt, Buehler, Stephanie, Echeverria, Diana, Fallacara, Dawn, and John, Lisa
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- 2021
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7. Comparison of sulfolane effects in Sprague Dawley rats, B6C3F1/N mice, and Hartley guinea pigs after 28 days of exposure via oral gavage
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Shipkowski, K.A., Cora, M.C., Cesta, M.F., Robinson, V.G., Waidyanatha, S., Witt, K.L., Vallant, M.K., Fallacara, D.M., Hejtmancik, M.R., Masten, S.A., Cooper, S.D., Fernando, R.A., and Blystone, C.R.
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- 2021
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8. Comparison of sulfolane effects in Sprague Dawley rats, B6C3F1/N mice, and Hartley guinea pigs after 28 days of exposure via oral gavage
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K.A. Shipkowski, M.C. Cora, M.F. Cesta, V.G. Robinson, S. Waidyanatha, K.L. Witt, M.K. Vallant, D.M. Fallacara, M.R. Hejtmancik, S.A. Masten, S.D. Cooper, R.A. Fernando, and C.R. Blystone
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Sulfolane ,Rat ,Mice ,Guinea pig ,Kidney ,Plasma concentration ,Toxicology. Poisons ,RA1190-1270 - Abstract
Sulfolane is a solvent used in industrial refining with identified environmental exposure in drinking water. Due to potential large species differences, the National Toxicology Program (NTP) conducted 28-day toxicity studies in male and female Hsd:Sprague Dawley® SD® rats, B6C3F1/N mice, and Hartley guinea pigs. A wide dose range of 0, 1, 10, 30, 100, 300, and 800 mg/kg was administered via gavage. Histopathology, clinical pathology, and organ weights were evaluated after 28 days of exposure. In addition, plasma concentrations of sulfolane were evaluated 2 and 24 h after the last dose. Increased mortality was observed in the highest dose group of guinea pigs and mice while decreased body weight was observed in rats compared to controls. Histopathological lesions were observed in the kidney (male rat), stomach (male mice), esophagus (male and female guinea pigs), and nose (male guinea pigs). Plasma concentrations were generally higher in rats and guinea pigs compared to mice with evidence of saturated clearance at higher doses. Male rats appear to be the most sensitive with hyaline droplet accumulation occurring at all doses, although the human relevance of this finding is questionable.
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- 2021
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9. Immunotoxicity studies of sulfolane following developmental exposure in Hsd:Sprague Dawley SD rats and adult exposure in B6C3F1/N mice
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AtLee T. D. Watson, Victor J. Johnson, Michael I. Luster, Gary R. Burleson, Dawn M. Fallacara, Barney R. Sparrow, Mark F. Cesta, Michelle C. Cora, Keith R. Shockley, Matt D. Stout, Chad R. Blystone, and Dori R. Germolec
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immunotoxicity ,sulfolane ,immune system ,immunotoxicology ,nk cells ,innate immunity ,Immunologic diseases. Allergy ,RC581-607 ,Toxicology. Poisons ,RA1190-1270 - Abstract
Sulfolane is a solvent used in the petrochemical industry and a groundwater contaminant in areas near refineries. The current studies were conducted to assess the impact of oral exposure to sulfolane on the immune system using two models: (1) a perinatal drinking water exposure to 0, 30, 100, 300, or 1000 mg/L from gestation day (GD) 6 until ∼13 weeks-of-age in Harlan Sprague Dawley rats; and, (2) a 90-day gavage exposure of adult female B6C3F1/N mice to 0, 1, 10, 30, 100, or 300 mg/kg/day. Immune parameters evaluated included measurement of antibody production against sheep red blood cells (SRBC) and keyhole limpet hemocyanin (KLH), ex vivo measurements of natural killer (NK) cell activity, cytotoxic T-cell (CTL) activity, and T-cell proliferation, as well as measures of splenic immune cell populations, hematological parameters, and histopathology of immune tissues. A decrease in ex vivo NK cell activity was observed in cells from female – but not male – F1 rats following developmental exposure. In adult female mice, splenic NK cell number was lower than the vehicle controls at doses ≥ 100 mg/kg; however, ex vivo NK cell activity was not affected by sulfolane treatment. In female mice, a decrease in the number of large unstained cells at doses ≥ 30 mg/kg was observed. In F1 rats, effects on white blood cells (WBC) were limited to a decreasing trend in leukocytes in females; no effects were observed in males. Under the conditions of this study, a no-observed-effect level (NOEL) of 3 mg/kg/day was identified based on reduced NK cell activity in female F1 rats. Overall, these findings suggest that oral exposure to sulfolane in rodents had minimal effects on the immune system.
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- 2021
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10. Perfluoroalkyl substances exposure and immunity, allergic response, infection, and asthma in children: review of epidemiologic studies
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Haley von Holst, Pratibha Nayak, Zygmunt Dembek, Stephanie Buehler, Diana Echeverria, Dawn Fallacara, and Lisa John
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PFAS ,Perfluoroalkyl substances ,Infant and child health ,Immune function ,Allergy ,Infection ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Increased exposure to perfluoroalkyl substances (PFAS) potentially affects infant and childhood health through immunosuppression. Given rapidly evolving research on PFAS, it is important to comprehensively examine the impact of PFAS exposure among the pediatric population as new research becomes available due to potential fragility of the developing immune system. Objectives: This review assessed the effects of PFAS fetal, infant and childhood exposures upon the development of immune function during early life stages. Methods: Researchers completed a literature review, searching PubMed for human studies published since 2010 for PFAS and health outcomes among infants and children. Included articles incorporated key search terms in the title or abstract; non-research reports and non-English papers were excluded. The search identified 518 studies for possible inclusion. Following hands-on review, 34 were determined relevant. Subsequent analyses found 8 additional relevant articles, totaling 42 studies. Results: Major immune-related sequelae from PFAS exposures on infant and child health outcomes documented in recent literature include:• Strong indication of immunosuppression, with diminished childhood antibody response to vaccination, particularly with PFOA, PFOS and PFHxS exposures.• Some indication of increased risks of childhood infectious diseases/infections, particularly from PFOS exposures.• Limited indication of an effect of PFAS exposure on allergic reactions/allergen specific IgE antibodies.• Limited indication of an effect of PFAS exposure on atopic dermatitis (AD).• Limited indication of an effect of PFAS exposure on asthma and lung function. Conclusion: This review summarizes recent findings of PFAS effects on infant and childhood immune health. Evidence of immunosuppression, diminished vaccine efficacy, and increased risk of infections, allergies, asthma and AD were described following in utero, infant, and early childhood PFAS exposures. Further investigation is warranted to characterize PFAS exposure pathways and potential modes of action in relation to PFAS effects on the developing immune system. Incontrovertible proof of PFAS immunotoxic effects could optimally be obtained by a large prospective study cohort of mothers and children from infancy through school-age. Regular assessments of circulating antibodies and response to infant and childhood vaccines during growth years could prove invaluable.
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- 2021
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11. Natural history of disease in cynomolgus monkeys exposed to Ebola virus Kikwit strain demonstrates the reliability of this non-human primate model for Ebola virus disease.
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Nancy A Niemuth, Dawn Fallacara, Cheryl A Triplett, Sanjay M Tamrakar, Alisha Rajbhandari, Clint Florence, Lucy Ward, Anthony Griffiths, Ricardo Carrion, Yenny Goez-Gazi, Kendra J Alfson, Hilary M Staples, Trevor Brasel, Jason E Comer, Shane Massey, Jeanon Smith, Andrew Kocsis, Jake Lowry, Sara C Johnston, Aysegul Nalca, Arthur J Goff, Amy C Shurtleff, Margaret L Pitt, John Trefry, and Michael P Fay
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Medicine ,Science - Abstract
Filoviruses (Family Filoviridae genera Ebolavirus and Marburgvirus) are negative-stranded RNA viruses that cause severe health effects in humans and non-human primates, including death. Except in outbreak settings, vaccines and other medical countermeasures against Ebola virus (EBOV) will require testing under the FDA Animal Rule. Multiple vaccine candidates have been evaluated using cynomolgus monkeys (CM) exposed to EBOV Kikwit strain. To the best of our knowledge, however, animal model development data supporting the use of CM in vaccine research have not been submitted to the FDA. This study describes a large CM database (122 CM, 62 female and 60 male, age 2 to 9 years) and demonstrates the consistency of the CM model through time to death models and descriptive statistics. CMs were exposed to EBOV doses of 0.1 to 100,000 PFU in 33 studies conducted at three Animal Biosafety Level 4 facilities, by three exposure routes. Time to death was modeled using Cox proportional hazards models with a frailty term that incorporated study-to-study variability. Despite significant differences attributed to exposure variables, all CMs exposed to the 100 to 1,000 pfu doses commonly used in vaccine studies died or met euthanasia criteria within 21 days of exposure, median 7 days, 93% between 5 and 12 days of exposure. Moderate clinical signs were observed 4 to 5 days after exposure and preceded death or euthanasia by approximately one day. Viremia was detected within a few days of infection. Hematology indices were indicative of viremia and the propensity for hemorrhage with progression of Ebola viremia. Changes associated with coagulation parameters and platelets were consistent with coagulation disruption. Changes in leukocyte profiles were indicative of an acute inflammatory response. Increased liver enzymes were observed shortly after exposure. Taken together, these factors suggest that the cynomolgus monkey is a reliable animal model for human disease.
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- 2021
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12. The Pyrazolo[3,4-d]Pyrimidine Derivative Si306 Encapsulated into Anti-GD2-Immunoliposomes as Therapeutic Treatment of Neuroblastoma
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Enrico Rango, Fabio Pastorino, Chiara Brignole, Arianna Mancini, Federica Poggialini, Salvatore Di Maria, Claudio Zamperini, Giulia Iovenitti, Anna Lucia Fallacara, Samantha Sabetta, Letizia Clementi, Massimo Valoti, Silvia Schenone, Adriano Angelucci, Mirco Ponzoni, Elena Dreassi, and Maurizio Botta
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neuroblastoma ,c-Src inhibitor ,liposomes ,anti-GD2 monoclonal antibody ,immunoliposomes ,Biology (General) ,QH301-705.5 - Abstract
Si306, a pyrazolo[3,4-d]pyrimidine derivative recently identified as promising anticancer agent, has shown favorable in vitro and in vivo activity profile against neuroblastoma (NB) models by acting as a competitive inhibitor of c-Src tyrosine kinase. Nevertheless, Si306 antitumor activity is associated with sub-optimal aqueous solubility, which might hinder its further development. Drug delivery systems were here developed with the aim to overcome this limitation, obtaining suitable formulations for more efficacious in vivo use. Si306 was encapsulated in pegylated stealth liposomes, undecorated or decorated with a monoclonal antibody able to specifically recognize and bind to the disialoganglioside GD2 expressed by NB cells (LP[Si306] and GD2-LP[Si306], respectively). Both liposomes possessed excellent morphological and physio-chemical properties, maintained over a period of two weeks. Compared to LP[Si306], GD2-LP[Si306] showed in vitro specific cellular targeting and increased cytotoxic activity against NB cell lines. After intravenous injection in healthy mice, pharmacokinetic profiles showed increased plasma exposure of Si306 when delivered by both liposomal formulations, compared to that obtained when Si306 was administered as free form. In vivo tumor homing and cytotoxic effectiveness of both liposomal formulations were finally tested in an orthotopic animal model of NB. Si306 tumor uptake resulted significantly higher when encapsulated in GD2-LP, compared to Si306, either free or encapsulated into untargeted LP. This, in turn, led to a significant increase in survival of mice treated with GD2-LP[Si306]. These results demonstrate a promising antitumor efficacy of Si306 encapsulated into GD2-targeted liposomes, supporting further therapeutic developments in pre-clinical trials and in the clinic for NB.
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- 2022
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13. An Unfinished Manifesto for Stereotomy 2.0
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Fallacara, Giuseppe and Barberio, Maurizio
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- 2018
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14. Survey of Parasites and Bacterial Pathogens from Free-Living Waterfowl in Zoological Settings
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Fallacara, Dawn M., Monahan, Clifton M., Morishita, Teresa Y., Bremer, Catherine A., and Wack, Raymond F.
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- 2004
15. Fecal Shedding and Antimicrobial Susceptibility of Selected Bacterial Pathogens and a Survey of Intestinal Parasites in Free-Living Waterfowl
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Fallacara, D. M., Monahan, C. M., Morishita, T. Y., and Wack, R. F.
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- 2001
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16. Consejo Asociado Don José: relato de una experiencia
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Romina Fallacara
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General Medicine - Abstract
En agosto del 2018, mediante trabajo conjunto entre la Municipalidad de Florencio Varela, la Universidad Arturo Jauretche y la Facultad Latinoamericana de Ciencias Sociales (FLACSO), se inició una experiencia de política pública con métodos de planificación participativa y gestión asociada (PPGA) en el barrio Don José. FLACSO brindaría una transferencia metodológica basada en prácticas de intersectorialidad, interdisciplina y consenso. La primera etapa se centró en la salud y en la niñez/adolescencia, y en la segunda, desarrollada durante la pandemia de covid-19, se intentó salvar el colectivo de trabajo, a través de la virtualidad. En la actualidad, los proyectos asociados se encuentran avanzando y fortaleciéndose para la postpandemia, mientras que se desarrollan acciones concretas para beneficiar a la comunidad donde el Consejo Asociado funciona.
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- 2022
17. Validation of the Italian version of the Cluster Headache Impact Questionnaire (CHIQ)
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Onofri, Agnese, Iannone, Luigi Francesco, Granato, Antonio, Garascia, Gabriele, Bartole, Luca, Manganotti, Paolo, Vollono, Catello, Romozzi, Marina, Sottani, Costanza, Calabresi, Paolo, Tassorelli, Cristina, Sances, Grazia, Allena, Marta, De Icco, Roberto, De Cesaris, Francesco, Burgalassi, Andrea, Chiarugi, Alberto, Baraldi, Carlo, Guerzoni, Simona, Prudenzano, Maria Pia, Fallacara, Adriana, Albanese, Maria, Rainero, Innocenzo, Coppola, Gianluca, Casalena, Alfonsina, Mampreso, Edoardo, Pistoia, Francesca, Sarchielli, Paola, Morson, Marisa, Sacco, Simona, Geppetti, Pierangelo, Ornello, Raffaele, Calabresi, Paolo (ORCID:0000-0003-0326-5509), Onofri, Agnese, Iannone, Luigi Francesco, Granato, Antonio, Garascia, Gabriele, Bartole, Luca, Manganotti, Paolo, Vollono, Catello, Romozzi, Marina, Sottani, Costanza, Calabresi, Paolo, Tassorelli, Cristina, Sances, Grazia, Allena, Marta, De Icco, Roberto, De Cesaris, Francesco, Burgalassi, Andrea, Chiarugi, Alberto, Baraldi, Carlo, Guerzoni, Simona, Prudenzano, Maria Pia, Fallacara, Adriana, Albanese, Maria, Rainero, Innocenzo, Coppola, Gianluca, Casalena, Alfonsina, Mampreso, Edoardo, Pistoia, Francesca, Sarchielli, Paola, Morson, Marisa, Sacco, Simona, Geppetti, Pierangelo, Ornello, Raffaele, and Calabresi, Paolo (ORCID:0000-0003-0326-5509)
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Background: The Cluster Headache Impact Questionnaire (CHIQ) is a specific and easy-to-use questionnaire to assess the current impact of cluster headache (CH). The aim of this study was to validate the Italian version of the CHIQ. Methods: We included patients diagnosed with episodic CH (eCH) or chronic CH (cCH) according to the ICHD-3 criteria and included in the "Italian Headache Registry" (RICe). The questionnaire was administered to patients through an electronic form in two sessions: at first visit for validation, and after 7 days for test-retest reliability. For internal consistency, Cronbach's alpha was calculated. Convergent validity of the CHIQ with CH features and the results of questionnaires assessing anxiety, depression, stress, and quality of life was evaluated using Spearman's correlation coefficient. Results: We included 181 patients subdivided in 96 patients with active eCH, 14 with cCH, and 71 with eCH in remission. The 110 patients with either active eCH or cCH were included in the validation cohort; only 24 patients with CH were characterized by a stable attack frequency after 7 days, and were included in the test-retest cohort. Internal consistency of the CHIQ was good with a Cronbach alpha value of 0.891. The CHIQ score showed a significant positive correlation with anxiety, depression, and stress scores, while showing a significant negative correlation with quality-of-life scale scores. Conclusion: Our data show the validity of the Italian version of the CHIQ, which represents a suitable tool for evaluating the social and psychological impact of CH in clinical practice and research.
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- 2023
18. 14-day toxicity studies of tetravalent and pentavalent vanadium compounds in Harlan Sprague Dawley rats and B6C3F1/N mice via drinking water exposure
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Roberts, Georgia K., Stout, Matthew D., Sayers, Brian, Fallacara, Dawn M., Hejtmancik, Milton R., Waidyanatha, Suramya, and Hooth, Michelle J.
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- 2016
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19. Time Series Clustering of Online Gambling Activities for Addicted Users’ Detection
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Fernando Peres, Enrico Fallacara, Luca Manzoni, Mauro Castelli, Aleš Popovič, Miguel Rodrigues, and Pedro Estevens
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human behavior modeling ,online gambling ,machine learning ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Ever since the worldwide demand for gambling services started to spread, its expansion has continued steadily. To wit, online gambling is a major industry in every European country, generating billions of Euros in revenue for commercial actors and governments alike. Despite such evidently beneficial effects, online gambling is ultimately a vast social experiment with potentially disastrous social and personal consequences that could result in an overall deterioration of social and familial relationships. Despite the relevance of this problem in society, there is a lack of tools for characterizing the behavior of online gamblers based on the data that are collected daily by betting platforms. This paper uses a time series clustering algorithm that can help decision-makers in identifying behaviors associated with potential pathological gamblers. In particular, experimental results obtained by analyzing sports event bets and black jack data demonstrate the suitability of the proposed method in detecting critical (i.e., pathological) players. This algorithm is the first component of a system developed in collaboration with the Portuguese authority for the control of betting activities.
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- 2021
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20. Long‐term (48 weeks) effectiveness, safety, and tolerability of erenumab in the prevention of high‐frequency episodic and chronic migraine in a real world: Results of the EARLY 2 study
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Barbanti P., Aurilia C., Cevoli S., Egeo G., Fofi L., Messina R., Salerno A., Torelli P., Albanese M., Carnevale A., Bono F., D'Amico D., Filippi M., Altamura C., Vernieri F., Colombo B., Frediani F., Mercuri B., D'Onofrio F., Grazzi L., Aguggia M., Pierangeli G., Favoni V., Finocchi C., Di Fiore P., Costa C. M., Brunelli N., Fallacara A., Bertuzzo D., Zucco M., Di Clemente L., Trimboli M., Pascarella A., Manzo L., Barbanti P., Aurilia C., Cevoli S., Egeo G., Fofi L., Messina R., Salerno A., Torelli P., Albanese M., Carnevale A., Bono F., D'Amico D., Filippi M., Altamura C., Vernieri F., Colombo B., Frediani F., Mercuri B., D'Onofrio F., Grazzi L., Aguggia M., Pierangeli G., Favoni V., Finocchi C., Di Fiore P., Costa C.M., Brunelli N., Fallacara A., Bertuzzo D., Zucco M., Di Clemente L., Trimboli M., Pascarella A., Manzo L., Barbanti, Piero, Aurilia, Cinzia, Cevoli, Sabina, Egeo, Gabriella, Fofi, Luisa, Messina, Roberta, Salerno, Antonio, Torelli, Paola, Albanese, Maria, Carnevale, Antonio, Bono, Francesco, D'Amico, Domenico, Filippi, Massimo, Altamura, Claudia, and Vernieri, Fabrizio
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Adult ,Male ,Calcitonin Gene-Related Peptide Receptor Antagonist ,medicine.medical_specialty ,Visual analogue scale ,Migraine Disorders ,Population ,Analgesic ,Longitudinal Studie ,Sex Factor ,Calcitonin gene-related peptide ,Antibodies, Monoclonal, Humanized ,calcitonin gene-related peptide ,Sex Factors ,Chronic Migraine ,Migraine Disorder ,Calcitonin Gene-Related Peptide Receptor Antagonists ,Interquartile range ,Internal medicine ,Outcome Assessment, Health Care ,sex ,Humans ,Medicine ,long-term treatment ,migraine ,Longitudinal Studies ,education ,allodynia ,education.field_of_study ,business.industry ,Middle Aged ,medicine.disease ,Italy ,Neurology ,Tolerability ,Migraine ,erenumab ,Hyperalgesia ,Chronic Disease ,Female ,Neurology (clinical) ,business ,Human - Abstract
Objective: To evaluate the long-term effectiveness, safety, and tolerability of erenumab in a real-world migraine population, looking for putative predictors of responsiveness. Background: Erenumab proved to be effective, safe, and well tolerated in the prevention of episodic migraine (EM) and chronic migraine (CM) in long-term extension studies of double-blind, placebo-controlled trials in patients with no more than two (EM) or three (CM) prior preventive treatment failures. Methods: A 48-week, multicenter, longitudinal cohort real-life study was conducted at 15headache centers across eight Italian regions between December 20, 2018 and July 31, 2020. We considered all consecutive patients with high-frequency episodic migraine (HFEM) or CM aged 18–65years. Each patient was treated with erenumab 70mg, administered monthly. The dose was switched to 140mg in nonresponders and in responders who had become nonresponders for at least 4weeks. Change in monthly migraine days (MMDs) or monthly headache days (MHDs) at Weeks 45–48 compared with baseline was the primary efficacy endpoint. Secondary endpoints encompassed variation in monthly analgesic intake, achievement of a ≥50%, ≥75%, or 100% reduction in migraine or headache days, and any change in the Visual Analogue Scale (VAS) and Headache Impact Test-6 scores (HIT-6) during the same time interval. Results: A total of 242 patients with migraine received at least one dose of erenumab 70mg and were considered for safety analysis, whereas 221 received a monthly erenumab dose for ≥48weeks and were included in the effectiveness and safety analysis set. All patients had previously been treated unsuccessfully with ≥3migraine-preventive medication classes. From baseline to Weeks 45–48, erenumab treatment reduced MMD by 4.3±5.3(mean±SD) in patients with HFEM, and MHD by 12.8±8.9 (mean±SD) in subjects with CM. VAS and HIT-6scores were decreased by 1.8±1.9 (mean±SD) and 12.3±11 (mean±SD) in HFEM, and by 3.0±2.2 (mean±SD) and 13.1±11.2 (mean±SD) in CM. Median monthly analgesic intake passed from 11.0 (interquartile range [IQR] 10.0–13.0) to 5 (IQR 2.0–8.0) in HFEM and from 20.0 (IQR 15.0–30.0) to 6.0 (IQR 3.8–10.0) in CM. The ≥50% responders were 56.1% (32/57) in HFEM and 75.6% (124/164) in CM; ≥75% responders were 31.6% (18/57) and 44.5% (73/164); and 100% responders were 8.8% (5/57) and 1.2% (2/164), respectively. At Week 48, 83.6% (137/164) of patients with CM had reverted to EM. Erenumab was safe and well tolerated. Responsiveness to erenumab was positively associated with cutaneous allodynia (OR: 5.44, 95% CI: 1.52–19.41; p=0.009) in HFEM. In patients with CM, ≥50% responsiveness was positively associated with male sex (OR: 2.99, 95% CI: 1.03–8.7; p=0.044) and baseline migraine frequency (OR: 1.12, 95% CI: 1.05–1.20; p=0.001) and negatively associated with psychiatric comorbidities (OR: 0.37, 95% CI: 0.15–0.87; p=0.023) and prior treatment failures (OR: 0.77, 95% CI: 0.64–0.92; p=0.004). Conclusions: Long-term (48-week) erenumab treatment provides sustained effectiveness, safety, and tolerability in real-life patients with HFEM or CM with ≥3 prior preventive treatment failures. The dose of 140mg was required in most patients along the study and should be taken into consideration as the starting dose. Allodynia (in HFEM), male sex, and baseline migraine frequency (in CM) might represent positive responsiveness predictors. Conversely, psychiatric comorbidities and multiple prior preventive treatment failures could be negative predictors in patients with CM.
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- 2021
21. 14-day toxicity studies of tetravalent and pentavalent vanadium compounds in Harlan Sprague Dawley rats and B6C3F1/N mice via drinking water exposure
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Georgia K. Roberts, Matthew D. Stout, Brian Sayers, Dawn M. Fallacara, Milton R. Hejtmancik, Suramya Waidyanatha, and Michelle J. Hooth
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Toxicology. Poisons ,RA1190-1270 - Abstract
Background: The National Toxicology Program (NTP) performed short-term toxicity studies of tetra- and pentavalent vanadium compounds, vanadyl sulfate and sodium metavanadate, respectively. Due to widespread human exposure and a lack of chronic toxicity data, there is concern for human health following oral exposure to soluble vanadium compounds. Objectives: To compare the potency and toxicological profile of vanadyl sulfate and sodium metavanadate using a short-term in vivo toxicity assay. Methods: Adult male and female Harlan Sprague Dawley (HSD) rats and B6C3F1/N mice, 5 per group, were exposed to vanadyl sulfate or sodium metavanadate, via drinking water, at concentrations of 0, 125, 250, 500, 1000 or 2000 mg/L for 14 days. Water consumption, body weights and clinical observations were recorded throughout the study; organ weights were collected at study termination. Results: Lower water consumption, up to −80% at 2000 mg/L, was observed at most exposure concentrations for animals exposed to either vanadyl sulfate or sodium metavanadate and was accompanied by decreased body weights at the highest concentrations for both compounds. Animals in the 1000 and 2000 mg/L sodium metavanadate groups were removed early due to overt toxicity. Thinness was observed in high-dose animals exposed to either compound, while lethargy and abnormal gait were only observed in vanadate-exposed animals. Conclusions: Based on clinical observations and overt toxicity, sodium metavanadate appears to be more toxic than vanadyl sulfate. Differential toxicity cannot be explained by differences in total vanadium intake, based on water consumption, and may be due to differences in disposition or mechanism of toxicity. Keywords: Pentavalent vanadium, Tetravalent vanadium, Vanadyl sulfate, Sodium metavanadate, B6C3F1/N, Harlan Sprague-Dawley, Short-term toxicity, National Toxicology Program
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- 2016
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22. Locking down the CGRP pathway during the COVID-19 pandemic lockdown: the PandeMig study
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Adriana Fallacara, Sabina Cevoli, Paola Torelli, Claudia Altamura, Gabriella Egeo, Piero Barbanti, Giulia Pierangeli, Cinzia Aurilia, Fabrizio Vernieri, Umberto Pensato, Nicoletta Brunelli, Luisa Fofi, Valentina Favoni, Altamura C., Cevoli S., Aurilia C., Egeo G., Fofi L., Torelli P., Brunelli N., Pierangeli G., Favoni V., Fallacara A., Pensato U., Barbanti P., and Vernieri F.
- Subjects
Male ,Neurology ,Longitudinal Studie ,Cohort Studies ,0302 clinical medicine ,Migraine Disorder ,Surveys and Questionnaires ,Pandemic ,Longitudinal Studies ,030212 general & internal medicine ,CGRP ,General Medicine ,Middle Aged ,Psychiatry and Mental health ,Italy ,Cohort ,Quarantine ,Female ,Neurosurgery ,Coronavirus Infections ,Cohort study ,Human ,Adult ,medicine.medical_specialty ,Calcitonin Gene-Related Peptide Receptor Antagonist ,Migraine Disorders ,Pneumonia, Viral ,Clinical Neurology ,Dermatology ,Calcitonin gene-related peptide ,Antibodies, Monoclonal, Humanized ,03 medical and health sciences ,Calcitonin Gene-Related Peptide Receptor Antagonists ,Internal medicine ,medicine ,Humans ,Pandemics ,Migraine ,business.industry ,Coronavirus Infection ,COVID-19 ,medicine.disease ,Galcanezumab ,Presenteeism ,Neurology (clinical) ,Cohort Studie ,business ,030217 neurology & neurosurgery ,Erenumab - Abstract
Objectives: The COVID-19 pandemic and the consequent lockdown came as a storm disrupting people’s everyday life. This study aimed at observing whether the COVID-19 related lockdown influenced migraine frequency and disability in migraine patients on therapy with monoclonal antibodies inhibiting the CGRP pathway. Methods: In this longitudinal observational cohort study, 147 consecutive patients receiving monthly administration of erenumab or galcanezumab were enrolled in four Italian headache centers. All patients filled a questionnaire concerning working and household settings, recent flu symptoms or COVID-19 diagnosis, and family loss due to COVID-19 infection. Monthly migraine days (MMDs), monthly painkiller intake (MPI), and HIT-6 disability relative to the first month of lockdown imposition (T-lock) and the month before (T-free) were also collected. Results: From T-free to T-lock, the cohort displayed a reduction in MMDs (from 10.5 ± 7.6 to 9.8 ± 7.6, p =.024) and HIT-6 scores (from 59.3 ± 8.3 men reduced MPI more frequently than women (p =.005). Conclusions: Our study observed that the lockdown impact to 57.8 ± 8.8, p=.009), while MPI resulted unchanged (from 11.6 ± 11.5 to 11.1 ± 11.7; p =.114). MMDs, MPI, and HIT-6 variations from T-free to T-lock did not differ according to work settings or household. Patients beyond the first 3 months of therapy presented less often a reduction in MMDs (p =.006) and on everyday life did not affect the migraine load in patients receiving monoclonal antibodies inhibiting the CGRP pathway. Patients in the first months of therapy experienced a greater improvement according to drug pharmacokinetics, while women more frequently needed rescue medications, possibly indicating presenteeism or cephalalgophobia.
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- 2020
23. Cover the Antique. The case of the Arena of Verona
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Giuseppe Fallacara, Ubaldo Occhinegro, and Micaela Pignatelli
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Arena di Verona ,kinetic architecture ,large shell ,reversible architecture ,Architecture ,NA1-9428 - Abstract
The International Competition for Verona Arena Coverage has animated the international debate on the theme of contemporary intervention on ancient architecture. The NFRG of Politecnico of Bari has proposed an experimental, reversible, modular and structurally independent roofing solution. Starting from the study of the velaria, the group has come to a design research inspired by the Kinetic Architecture.
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- 2017
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24. Proton Therapy and Src Family Kinase Inhibitor Combined Treatments on U87 Human Glioblastoma Multiforme Cell Line
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Francesco P Cammarata, Filippo Torrisi, Giusi I Forte, Luigi Minafra, Valentina Bravatà, Pietro Pisciotta, Gaetano Savoca, Marco Calvaruso, Giada Petringa, Giuseppe A. P. Cirrone, Anna L Fallacara, Laura Maccari, Maurizio Botta, Silvia Schenone, Rosalba Parenti, Giacomo Cuttone, and Giorgio Russo
- Subjects
glioblastoma multiforme ,proton therapy ,combined treatments ,gene signatures ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Glioblastoma Multiforme (GBM) is the most common of malignant gliomas in adults with an exiguous life expectancy. Standard treatments are not curative and the resistance to both chemotherapy and conventional radiotherapy (RT) plans is the main cause of GBM care failures. Proton therapy (PT) shows a ballistic precision and a higher dose conformity than conventional RT. In this study we investigated the radiosensitive effects of a new targeted compound, SRC inhibitor, named Si306, in combination with PT on the U87 glioblastoma cell line. Clonogenic survival assay, dose modifying factor calculation and linear-quadratic model were performed to evaluate radiosensitizing effects mediated by combination of the Si306 with PT. Gene expression profiling by microarray was also conducted after PT treatments alone or combined, to identify gene signatures as biomarkers of response to treatments. Our results indicate that the Si306 compound exhibits a radiosensitizing action on the U87 cells causing a synergic cytotoxic effect with PT. In addition, microarray data confirm the SRC role as the main Si306 target and highlights new genes modulated by the combined action of Si306 and PT. We suggest, the Si306 as a new candidate to treat GBM in combination with PT, overcoming resistance to conventional treatments.
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- 2019
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25. Formulation and Characterization of Native and Crosslinked Hyaluronic Acid Microspheres for Dermal Delivery of Sodium Ascorbyl Phosphate: A Comparative Study
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Arianna Fallacara, Filippo Marchetti, Michele Pozzoli, Ugo Raffaello Citernesi, Stefano Manfredini, and Silvia Vertuani
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dermal delivery ,drug release ,hyaluronic acid ,urea-crosslinked hyaluronic acid ,microspheres ,sodium ascorbyl phosphate ,Pharmacy and materia medica ,RS1-441 - Abstract
The present work evaluates for the first time the use of urea-crosslinked hyaluronic acid (HA-CL), a novel derivative of native hyaluronic acid (HA), to produce microspheres (MS) by emulsification-solvent evaporation, for dermal delivery of sodium ascorbyl phosphate (SAP). As the term of comparison, HA MS were prepared. A pre-formulation study—investigation of the effects of polymers solutions properties (pH, viscosity) and working conditions—led to the production of optimized HA-CL MS and HA-CL—SAP MS with: almost unimodal size distributions; mean diameter of 13.0 ± 0.7 and 9.9 ± 0.8 µm, respectively; spherical shape and rough surface; high yield, similar to HA MS and HA⁻SAP MS (≈ 85%). SAP was more efficiently encapsulated into HA-CL MS (78.8 ± 2.6%) compared to HA MS (69.7 ± 4.6%). Physical state, thermal properties, relative moisture stability of HA-CL MS and HA-CL⁻SAP MS were comparable to those of HA MS and HA⁻SAP MS. However, HA-CL⁻SAP MS exhibited an extended drug release compared to HA⁻SAP MS, despite the same kinetic mechanism—contemporaneous drug diffusion and polymer swelling/dissolution. Therefore, HA-CL formulation showed a greater potential as microcarrier (for encapsulation efficiency and release kinetic), that could be improved, in future, using suitable excipients.
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- 2018
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26. Immunotoxicity studies of sulfolane following developmental exposure in Hsd:Sprague Dawley SD rats and adult exposure in B6C3F1/N mice
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Keith R. Shockley, Barney Sparrow, Victor J. Johnson, Matt D. Stout, Chad R. Blystone, Gary R. Burleson, Dawn Fallacara, Michael I. Luster, Dori R. Germolec, Mark F. Cesta, Michelle C. Cora, and AtLee T. D. Watson
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Male ,medicine.medical_specialty ,Immunology ,Mice, Inbred Strains ,Thiophenes ,Immunotoxicology ,Biology ,Toxicology ,Article ,sulfolane ,Rats, Sprague-Dawley ,Mice ,Immune system ,Pregnancy ,Internal medicine ,RA1190-1270 ,medicine ,Animals ,Cytotoxic T cell ,nk cells ,innate immunity ,Sheep ,Innate immune system ,immunotoxicity ,RC581-607 ,Rats ,immunotoxicology ,immune system ,Endocrinology ,Toxicology. Poisons ,biology.protein ,Gestation ,Female ,Histopathology ,Immunologic diseases. Allergy ,Spleen ,Ex vivo ,Keyhole limpet hemocyanin - Abstract
Sulfolane is a solvent used in the petrochemical industry and a groundwater contaminant in areas near refineries. The current studies were conducted to assess the impact of oral exposure to sulfolane on the immune system using two models: (1) a perinatal drinking water exposure to 0, 30, 100, 300, or 1000 mg/L from gestation day (GD) 6 until ~ 13 weeks-of-age in Harlan Sprague Dawley rats; and, (2) a 90-day gavage exposure of adult female B6C3F1/N mice to 0, 1, 10, 30, 100, or 300 mg/kg/day. Immune parameters evaluated included measurement of antibody production against sheep red blood cells (SRBC) and keyhole limpet hemocyanin (KLH), ex vivo measurements of natural killer (NK) cell activity, cytotoxic T-cell (CTL) activity, and T-cell proliferation, as well as measures of splenic immune cell populations, hematological parameters, and histopathology of immune tissues. A decrease in ex vivo NK cell activity was observed in cells from female – but not male – F1 rats following developmental exposure. In adult female mice, splenic NK cell number was lower than the vehicle controls at doses 100 ≥ mg/kg; however, ex vivo NK cell activity was not affected by sulfolane treatment. In female mice, a decrease in the number of large unstained cells at doses ≥ 30 mg/kg was observed. In F1 rats, effects on white blood cells (WBC) were limited to a decreasing trend in leukocytes in females; no effects were observed in males. Under the conditions of this study, a no-observed-effect level (NOEL) of 3 mg/kg/day was identified based on reduced NK cell activity in female F1 rats. Overall, these findings suggest that oral exposure to sulfolane in rodents had minimal effects on the immune system.
- Published
- 2021
27. Systemic exposure and urinary excretion of vanadium following perinatal subchronic exposure to vanadyl sulfate and sodium metavanadate via drinking water
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Suramya Waidyanatha, Frank X. Weber, Dawn M. Fallacara, James M. Harrington, Keith Levine, Veronica G. Robinson, Barney R. Sparrow, Matthew D. Stout, Reshan Fernando, Michelle J. Hooth, Guanhua Xie, and Georgia K. Roberts
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Male ,Vanadium Compounds ,Drinking Water ,Sodium ,Animals ,Female ,Vanadium ,General Medicine ,Vanadates ,Toxicology ,Article ,Rats - Abstract
Vanadium is a ubiquitous environmental contaminant although there are limited data to assess potential adverse human health impact following oral exposure. In support of studies investigating the subchronic toxicity of vanadyl sulfate (V(4+)) and sodium metavanadate (V(5+)) following perinatal exposure via drinking water in male and female rats, we have determined the internal exposure and urinary excretion of total vanadium at the end of study. Water consumption decreased with increasing exposure concentration following exposure to both compounds. Plasma and urine vanadium concentration normalized to total vanadium consumed per day increased with the exposure concentration of vanadyl sulfate and sodium metavanadate suggesting absorption increased as the exposure concentration increased. Additionally, females had higher concentrations than males (in plasma only for vanadyl sulfate exposure). Animals exposed to sodium metavanadate had up to 3-fold higher vanadium concentration in plasma and urine compared to vanadyl sulfate exposed animals, when normalized to total vanadium consumed per day, demonstrating differential absorption, distribution, metabolism, and excretion properties between V(5+) and V(4+) compounds. These data will aid in the interpretation of animal toxicity data of V(4+) and V(5+) compounds and determine the relevance of animal toxicity findings to human exposures.
- Published
- 2022
28. The Pyrazolo[3,4-d]Pyrimidine Derivative Si306 Encapsulated into Anti-GD2-Immunoliposomes as Therapeutic Treatment of Neuroblastoma
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Rango, Enrico, primary, Pastorino, Fabio, additional, Brignole, Chiara, additional, Mancini, Arianna, additional, Poggialini, Federica, additional, Maria, Salvatore Di, additional, Zamperini, Claudio, additional, Iovenitti, Giulia, additional, Fallacara, Anna Lucia, additional, Sabetta, Samantha, additional, Clementi, Letizia, additional, Valoti, Massimo, additional, Schenone, Silvia, additional, Angelucci, Adriano, additional, Ponzoni, Mirco, additional, Dreassi, Elena, additional, and Botta, Maurizio, additional
- Published
- 2022
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29. Predictive Gene Signature for Pyrazolopyrimidine Derivative c-Src Inhibitor 10a Sensitivity in Melanoma Cells
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Baris Kucukkaraduman, Muhammad Waqas Akbar, Zeynep Ozge Ayyildiz, Can Turk, Ali O. Gure, Kerem Mert Senses, Michal Lotem, Murat Isbilen, Maurizio Botta, Anna Lucia Fallacara, Kücükkaraduman, Barış, Akbar, Muhammad W., and Güre, Ali O.
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MAPK/ERK pathway ,Valproic Acid ,business.industry ,medicine.drug_class ,Sensitivity biomarkers ,Melanoma ,Organic Chemistry ,Mesenchymal stem cell ,Histone deacetylase inhibitor ,c-Src ,Drug resistance ,Gene signature ,medicine.disease ,Biochemistry ,3. Good health ,Drug Discovery ,Valproic acid ,Cancer research ,Medicine ,business ,Gene ,medicine.drug - Abstract
[Image: see text] Melanoma is a highly aggressive cancer with poor prognosis. Although more than 80% of melanomas harbor an activating mutation in genes within the MAPK pathway, which are mutually exclusive, usefulness of therapies targeting MAPK pathway are impeded by innate and/or acquired resistance in most patients. In this study, using melanoma cells, we report the efficacy of a recently developed pyrazolo[3,4-d]pyrimidine derived c-Src inhibitor 10a and identify a molecular signature which is predictive of 10a chemosensitivity. We show that the expression of TMED7, PLOD2, XRCC5, and NSUN5 are candidate biomarkers for 10a sensitivity. Although an undifferentiated/mesenchymal/invasive status of melanoma cells is associated with resistance to 10a, we show here for the first time that melanoma cells can be sensitized to 10a via treatment with valproic acid, a histone deacetylase inhibitor.
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- 2020
30. The Pyrazolo[3,4
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Enrico, Rango, Fabio, Pastorino, Chiara, Brignole, Arianna, Mancini, Federica, Poggialini, Salvatore, Di Maria, Claudio, Zamperini, Giulia, Iovenitti, Anna Lucia, Fallacara, Samantha, Sabetta, Letizia, Clementi, Massimo, Valoti, Silvia, Schenone, Adriano, Angelucci, Mirco, Ponzoni, Elena, Dreassi, and Maurizio, Botta
- Abstract
Si306, a pyrazolo[3,4
- Published
- 2022
31. Optimization of Aminoimidazole Derivatives as Src Family Kinase Inhibitors
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Cinzia Maria Francini, Francesca Musumeci, Anna Lucia Fallacara, Lorenzo Botta, Alessio Molinari, Roberto Artusi, Laura Mennuni, Adriano Angelucci, and Silvia Schenone
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aminoimidazole ,SFK inhibitors ,neuroblastoma ,anticancer ,tyrosine kinases ,Organic chemistry ,QD241-441 - Abstract
Protein kinases have emerged as crucial targets for cancer therapy over the last decades. Since 2001, 40 and 39 kinase inhibitors have been approved by FDA and EMA, respectively, and the majority are antineoplastic drugs. Morevoer, many candidates are currently in clinical trials. We previously reported a small library of 4-aminoimidazole and 2-aminothiazole derivatives active as Src family kinase (SFK) inhibitors. Starting from these results, we decided to perform an optimization study applying a mix and match strategy to identify a more potent generation of 4-aminoimidazoles. Firstly, a computational study has been performed, then compounds showing the best predicted docking scores were synthesized and screened in a cell-free assay for their SFK inhibitory activity. All the new chemical entities showed IC50s in the nanomolar range, with 2–130 fold increased activities compared to the previously reported inhibitors. Finally, the most active compounds have been tested on three cancer cell lines characterized by Src hyperactivation. Compounds 4k and 4l showed an interesting antiproliferative activity on SH-SY5Y neuroblastoma (NB) cell line. In this assay, the compounds resulted more potent than dasatinib, a tyrosine kinase inhibitor approved for the treatment of leukemias and in clinical trials for NB.
- Published
- 2018
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32. Hyaluronic Acid in the Third Millennium
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Arianna Fallacara, Erika Baldini, Stefano Manfredini, and Silvia Vertuani
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biological activity ,crosslinking ,drug delivery ,cosmetic ,food-supplement ,functionalization ,hyaluronan applications ,hyaluronan derivatives ,hyaluronan synthases ,hyaluronic acid ,hyaluronidases ,physico-chemical properties ,Organic chemistry ,QD241-441 - Abstract
Since its first isolation in 1934, hyaluronic acid (HA) has been studied across a variety of research areas. This unbranched glycosaminoglycan consisting of repeating disaccharide units of N-acetyl-d-glucosamine and d-glucuronic acid is almost ubiquitous in humans and in other vertebrates. HA is involved in many key processes, including cell signaling, wound reparation, tissue regeneration, morphogenesis, matrix organization and pathobiology, and has unique physico-chemical properties, such as biocompatibility, biodegradability, mucoadhesivity, hygroscopicity and viscoelasticity. For these reasons, exogenous HA has been investigated as a drug delivery system and treatment in cancer, ophthalmology, arthrology, pneumology, rhinology, urology, aesthetic medicine and cosmetics. To improve and customize its properties and applications, HA can be subjected to chemical modifications: conjugation and crosslinking. The present review gives an overview regarding HA, describing its history, physico-chemical, structural and hydrodynamic properties and biology (occurrence, biosynthesis (by hyaluronan synthases), degradation (by hyaluronidases and oxidative stress), roles, mechanisms of action and receptors). Furthermore, both conventional and recently emerging methods developed for the industrial production of HA and its chemical derivatization are presented. Finally, the medical, pharmaceutical and cosmetic applications of HA and its derivatives are reviewed, reporting examples of HA-based products that currently are on the market or are undergoing further investigations.
- Published
- 2018
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33. Time series clustering of online gambling activities for addicted users’ detection
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Miguel Rodrigues, Enrico Fallacara, Mauro Castelli, Pedro Estevens, Aleš Popovič, Fernando Peres, Luca Manzoni, Peres, F., Fallacara, E., Manzoni, L., Castelli, M., Popovic, A., Rodrigues, M., Estevens, P., NOVA Information Management School (NOVA IMS), NOVA IMS Research and Development Center (MagIC), and Information Management Research Center (MagIC) - NOVA Information Management School
- Subjects
Internet privacy ,Control (management) ,udc:004:78 ,Online gambling ,030508 substance abuse ,Human behavior modeling ,lcsh:Technology ,lcsh:Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Materials Science(all) ,Machine learning ,Revenue ,General Materials Science ,Relevance (information retrieval) ,Cluster analysis ,lcsh:QH301-705.5 ,Instrumentation ,Engineering(all) ,Fluid Flow and Transfer Processes ,biology ,business.industry ,Event (computing) ,lcsh:T ,Process Chemistry and Technology ,General Engineering ,Euros ,biology.organism_classification ,language.human_language ,lcsh:QC1-999 ,030227 psychiatry ,Computer Science Applications ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,language ,Portuguese ,0305 other medical science ,Social experiment ,business ,lcsh:Engineering (General). Civil engineering (General) ,lcsh:Physics - Abstract
Peres, F., Fallacara, E., Manzoni, L., Castelli, M., Popovič, A., Rodrigues, M., & Estevens, P. (2021). Time series clustering of online gambling activities for addicted users’ detection. Applied Sciences (Switzerland), 11(5), [2397]. https://doi.org/10.3390/app11052397 Ever since the worldwide demand for gambling services started to spread, its expansion has continued steadily. To wit, online gambling is a major industry in every European country, generating billions of Euros in revenue for commercial actors and governments alike. Despite such evidently beneficial effects, online gambling is ultimately a vast social experiment with potentially disastrous social and personal consequences that could result in an overall deterioration of social and familial relationships. Despite the relevance of this problem in society, there is a lack of tools for characterizing the behavior of online gamblers based on the data that are collected daily by betting platforms. This paper uses a time series clustering algorithm that can help decision-makers in identifying behaviors associated with potential pathological gamblers. In particular, experimental results obtained by analyzing sports event bets and black jack data demonstrate the suitability of the proposed method in detecting critical (i.e., pathological) players. This algorithm is the first component of a system developed in collaboration with the Portuguese authority for the control of betting activities. publishersversion published
- Published
- 2021
34. Genetic and Antigenic Characterization of Infectious Bursal Disease Viruses Isolated in Italy during the Period 2002-2005
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Martin, Ana Moreno, Fallacara, Francesca, Barbieri, Ilaria, Tosi, Giovanni, Rivallan, Gaelle, Eterradossi, Nicolas, Ceruti, Raffaella, and Cordioli, Paolo
- Published
- 2007
35. Thermal and Nuclear Quantum Effects at the Antiferroelectric to Paraelectric Phase Transition in KOH and KOD Crystals
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Michele Ceotto, Philippe Depondt, Fabio Finocchi, Erika Fallacara, Simon Huppert, Institut des Nanosciences de Paris (INSP), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Oxydes en basses dimensions (INSP-E9), and Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Phase transition ,Materials science ,Condensed matter physics ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,01 natural sciences ,Article ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Condensed Matter::Materials Science ,General Energy ,0103 physical sciences ,Thermal ,Antiferroelectricity ,Physical and Theoretical Chemistry ,[PHYS.COND]Physics [physics]/Condensed Matter [cond-mat] ,010306 general physics ,0210 nano-technology ,ComputingMilieux_MISCELLANEOUS - Abstract
Crystalline KOH undergoes an antiferroelectric (AFE) proton ordering phase transition at low temperatures, which results in a monoclinic bilayer structure held together by a network of weak hydrogen bonds (HBs). The Curie temperature shifts up when the compound is deuterated, an effect that classical MD is not able to catch. For deeper insights into the transition mechanism, we carry out ab initio MD simulations of KOH and KOD crystals by including quantum effects on the nuclei through Feynman path integrals. The geometric isotope effect and the evolution of the lattice parameters with temperature agree with the experimental data, while the purely classical description is not appropriate. Our results show that deuteration strengthens the HBs in the low-T AFE ordered phase. The transition is characterized by the flipping of OH/OD groups along a bending mode. Above the transition, the system is driven into a dynamical disordered paraelectric phase.
- Published
- 2021
36. The impact of electronic health records on healthcare quality: a systematic review and meta-analysis
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Campanella, Paolo, Lovato, Emanuela, Marone, Claudio, Fallacara, Lucia, Mancuso, Agostino, Ricciardi, Walter, and Specchia, Maria Lucia
- Published
- 2016
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37. Natural history of disease in cynomolgus monkeys exposed to Ebola virus Kikwit strain demonstrates the reliability of this non-human primate model for Ebola virus disease
- Author
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Lucy A Ward, Aysegul Nalca, Ricardo Carrion, Trevor Brasel, Arthur J. Goff, Hilary M. Staples, Alisha Rajbhandari, Margaret L. Pitt, Andrew Kocsis, John Trefry, Anthony Griffiths, Jason E. Comer, Cheryl A. Triplett, Nancy A. Niemuth, Clint Florence, Kendra J. Alfson, Dawn Fallacara, Amy C. Shurtleff, Shane Massey, Jake Lowry, Michael P. Fay, Sanjay M. Tamrakar, Yenny Goez-Gazi, Jeanon N. Smith, and Sara C. Johnston
- Subjects
0301 basic medicine ,RNA viruses ,Male ,Physiology ,Filoviridae ,medicine.disease_cause ,Pathology and Laboratory Medicine ,Body Temperature ,Disease Outbreaks ,0302 clinical medicine ,Medical Conditions ,Mathematical and Statistical Techniques ,Medicine and Health Sciences ,Medicine ,030212 general & internal medicine ,Vaccines ,Multidisciplinary ,biology ,Statistics ,Animal Models ,Viral Load ,Metaanalysis ,Ebolavirus ,Body Fluids ,Infectious Diseases ,Blood ,Physiological Parameters ,Experimental Organism Systems ,Medical Microbiology ,Filoviruses ,Viral Pathogens ,Viruses ,Physical Sciences ,Female ,Pathogens ,Anatomy ,Ebola Virus ,Viral load ,Research Article ,Infectious Disease Control ,Science ,Viremia ,Research and Analysis Methods ,Natural history of disease ,Microbiology ,03 medical and health sciences ,Virology ,Animals ,Statistical Methods ,Microbial Pathogens ,Ebola virus ,Biology and life sciences ,business.industry ,Hemorrhagic Fever Viruses ,Euthanasia ,Organisms ,Outbreak ,Reproducibility of Results ,Hemorrhagic Fever, Ebola ,biology.organism_classification ,Marburgvirus ,medicine.disease ,Blood Counts ,Disease Models, Animal ,Macaca fascicularis ,030104 developmental biology ,Animal Studies ,business ,Viral Transmission and Infection ,Mathematics - Abstract
Filoviruses (Family Filoviridae genera Ebolavirus and Marburgvirus) are negative-stranded RNA viruses that cause severe health effects in humans and non-human primates, including death. Except in outbreak settings, vaccines and other medical countermeasures against Ebola virus (EBOV) will require testing under the FDA Animal Rule. Multiple vaccine candidates have been evaluated using cynomolgus monkeys (CM) exposed to EBOV Kikwit strain. To the best of our knowledge, however, animal model development data supporting the use of CM in vaccine research have not been submitted to the FDA. This study describes a large CM database (122 CM, 62 female and 60 male, age 2 to 9 years) and demonstrates the consistency of the CM model through time to death models and descriptive statistics. CMs were exposed to EBOV doses of 0.1 to 100,000 PFU in 33 studies conducted at three Animal Biosafety Level 4 facilities, by three exposure routes. Time to death was modeled using Cox proportional hazards models with a frailty term that incorporated study-to-study variability. Despite significant differences attributed to exposure variables, all CMs exposed to the 100 to 1,000 pfu doses commonly used in vaccine studies died or met euthanasia criteria within 21 days of exposure, median 7 days, 93% between 5 and 12 days of exposure. Moderate clinical signs were observed 4 to 5 days after exposure and preceded death or euthanasia by approximately one day. Viremia was detected within a few days of infection. Hematology indices were indicative of viremia and the propensity for hemorrhage with progression of Ebola viremia. Changes associated with coagulation parameters and platelets were consistent with coagulation disruption. Changes in leukocyte profiles were indicative of an acute inflammatory response. Increased liver enzymes were observed shortly after exposure. Taken together, these factors suggest that the cynomolgus monkey is a reliable animal model for human disease.
- Published
- 2021
38. Novel Artificial Tears Containing Cross-Linked Hyaluronic Acid: An In Vitro Re-Epithelialization Study
- Author
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Arianna Fallacara, Silvia Vertuani, Giacomo Panozzo, Alessandra Pecorelli, Giuseppe Valacchi, and Stefano Manfredini
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anti-inflammatory ,artificial tears ,corneal epithelium ,cyclin D1 ,dry eye syndrome ,HA ,HA-CL ,IL-8 ,re-epithelialization ,Organic chemistry ,QD241-441 - Abstract
Dry eye syndrome is a common disease which can damage the corneal epithelium. It is treated with eye drops to stimulate tear production and hydrate the corneal surface. The most prescribed artificial tear remedies contain hyaluronic acid (HA), which enhances epithelial wound healing, improving tissue health. To the best of our knowledge, only a few recent studies have investigated cross-linked HA (HA-CL) in eye drops for human applications. This work consists in an in vitro evaluation of the re-epithelialization ability of two different preparations containing a recently synthetized HA cross-linked with urea: 0.02% (w/v) HA-CL (solution 1, S1), and 0.4% (w/v) HA-CL (solution 2, S2). The study was conducted on both 2D human corneal cells (HCEpiC) and 3D reconstructed tissues of human corneal epithelium (HCE). Viability by 3(4,5-dimethylthiazol-2)2,5-diphenyltetrazolium bromide (MTT) test, pro-inflammatory cytokine release (interleukin-8, IL-8) by ELISA, and morphology by hematoxylin and eosin (HE) staining were evaluated. In addition, to understand the molecular basis of the re-epithelialization properties, cyclin D1 levels were assessed by western blot. The results showed no cellular toxicity, a slight decrease in IL-8 release, and restoration of epithelium integrity when the wounded 3D model was treated with S1 and S2. In parallel, cyclin D1 levels increased in cells treated with both S1 and S2.
- Published
- 2017
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39. Natural history of disease in cynomolgus monkeys exposed to Ebola virus Kikwit strain demonstrates the reliability of this non-human primate model for Ebola virus disease
- Author
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Niemuth, Nancy A., primary, Fallacara, Dawn, additional, Triplett, Cheryl A., additional, Tamrakar, Sanjay M., additional, Rajbhandari, Alisha, additional, Florence, Clint, additional, Ward, Lucy, additional, Griffiths, Anthony, additional, Carrion, Ricardo, additional, Goez-Gazi, Yenny, additional, Alfson, Kendra J., additional, Staples, Hilary M., additional, Brasel, Trevor, additional, Comer, Jason E., additional, Massey, Shane, additional, Smith, Jeanon, additional, Kocsis, Andrew, additional, Lowry, Jake, additional, Johnston, Sara C., additional, Nalca, Aysegul, additional, Goff, Arthur J., additional, Shurtleff, Amy C., additional, Pitt, Margaret L., additional, Trefry, John, additional, and Fay, Michael P., additional
- Published
- 2021
- Full Text
- View/download PDF
40. Chikungunya Virus in Aedes albopictus, Italy
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Paolo Bonilauri, Romeo Bellini, Mattia Calzolari, Raffaella Angelini, Luciano Venturi, Francesca Fallacara, Paolo Cordioli, Paola Angelini, Claudio Venturelli, Giuseppe Merialdi, and Michele Dottori
- Subjects
Chikungunya ,Italy ,Aedes albopictus ,letter ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2008
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41. The Vault of the Hôtel de Ville in Arles
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Fallacara, Giuseppe, Resta, Fiore, Spallucci, Nicoletta, and Tamboréro, Luc
- Published
- 2011
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42. Hypar Vault II
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Giuseppe Fallacara, Maurizio Barberio, Giuseppe Fallacara, Fallacara, Giuseppe, and Barberio, Maurizio
- Published
- 2018
43. Towards a 4.0 Mass Customized Wooden Housing in the Mediterranean Area: The Ecodomus Project
- Author
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Micaela Colella, Giuseppe Fallacara, Fabio Bianconi, Marco Filippucci, Colella, M., and Fallacara, G.
- Subjects
Architectural engineering ,Engineering ,Large-scale cross-laminated timber (CLT) project ,Industry 4.0 ,business.industry ,Wood joint systems ,Mass customization ,Large-scale cross-laminated timber (CLT) projects ,Wood design, Wood joint systems, Large-scale cross-laminated timber (CLT) projects, Industry 4.0, Mass customization ,Wood design ,Construction industry ,Sustainable design ,Mediterranean area ,Relevance (information retrieval) ,Architecture ,business - Abstract
The paper describes the necessary transition from the consolidated practices of contemporary residential architecture to innovative sustainable design and construction practices, which can be pursued by applying the principles of digital fabrication to the construction industry. The contribution is divided into two parts: the first investigates the theoretical-critical assumptions of research and the second part describes a design experience that led to the creation of a housing prototype with an irregular geometry made up of CLT panels. The paper will explain the need to overcome the current phase of experimentation using digital fabrication technologies applied to small pavilions with complex shapes or high budget, to move to their application on residential building. The application of these technologies to residential architecture is desirable both for the diffusion and for the relevance of the change it would produce.
- Published
- 2019
44. Topological interlocking blocks for architecture: From flat to curved morphologies
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Giuseppe Fallacara, Micaela Colella, Maurizio Barberio, Yuri Estrin, Yves Bréchet, John Dunlop, Peter Fratzl, Fallacara, Giuseppe, Barberio, Maurizio, and Colella, Micaela
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Engineering ,business.industry ,Architectural design ,Subject (philosophy) ,Architecture ,business ,Topology ,Theme (computing) ,Studio ,Interlocking - Abstract
The paper concerns the theme of topological interlocking blocks for architecture and the relationship between flat stereotomic assemblies and curved morphologies. After a brief history of the subject, theoretical foundations and speculative research are presented. The research includes several built full-scale prototypes and architectural elements. The last part of the chapter describes the didactic experiences concerning the theme, during the third year Architectural Design Studio held by the authors, at Politecnico di Bari, Italy.
- Published
- 2019
45. Time Series Clustering of Online Gambling Activities for Addicted Users’ Detection
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Peres, Fernando, primary, Fallacara, Enrico, additional, Manzoni, Luca, additional, Castelli, Mauro, additional, Popovič, Aleš, additional, Rodrigues, Miguel, additional, and Estevens, Pedro, additional
- Published
- 2021
- Full Text
- View/download PDF
46. Agricultura familiar y economía social y solidaria en Río Negro : la experiencia de COOPOP
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Fallacara, Malena Sofía, Gallo, Mahuén, Valgiusti, Renata, Diéguez, Ricardo, and Mosse, Luis
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Cooperativa de Provisión y Comercialización Agropecuaria, Apícola y Granjera Productores Orgánicos Patagónicos Limitada ,Social economy ,Estratégias de desenvolvimento ,Cooperatives ,Economía solidaria ,Economia social ,Cooperativas ,Economia solidária ,Development strategies ,Viedma (Argentine) ,Solidarity economy ,Family farming ,Economía social ,Estrategias de desarrollo ,Agricultura familiar ,Viedma (Argentina) - Abstract
Fallacara, M. S. (2018). Agricultura familiar y economía social y solidaria en Río Negro: la experiencia de COOPOP. (Trabajo final integrador). Universidad Nacional de Quilmes, Bernal, Argentina. Este trabajo se propone realizar un primer acercamiento a la experiencia de la Cooperativa de Provisión y Comercialización Agropecuaria Apícola y Granjera Productores Orgánicos Patagónicos Limitada, conocida como COOPOP, ubicada en Viedma (Provincia de Río Negro). La misma será analizada a partir del diálogo con las temáticas, nociones, conceptos y herramientas teóricas vinculadas a la Economía Social y Solidaria (ESS) y la Agricultura Familiar. Para este trabajo, recuperaremos la historia de esta experiencia cooperativa a través de los desafíos y problemáticas que enfrenta en la actualidad. Se buscará profundizar en las estrategias que desde COOPOP se desarrollan para lograr la sostenibilidad de esta experiencia. En este sentido, desde el abordaje del marco conceptual de la ESS y la agricultura familiar, analizaremos el concepto de sostenibilidad, a partir del circuito productivo del cual forma parte COOPOP. Este acercamiento, permitirá aproximarnos a la discusión de la comercialización, el consumo y la propiedad de la tierra, en tanto temáticas comunes a las experiencias de la agricultura familiar, que forman parte de los desafíos propios de la sostenibilidad en la ESS.
- Published
- 2020
47. Evaluation of 2-methoxy-4-nitroaniline (MNA) in hypersensitivity, 14-day subacute, reproductive, and genotoxicity studies
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Matthew D. Stout, Matthew Buccellato, Helen Cunny, David E. Malarkey, Kristine L. Witt, Shawn F. Harris, Dawn Fallacara, Rachel P. Frawley, Irene Inok Surh, Dori R. Germolec, Wimolnut Manheng, Kimber L. White, Greg Travlos, Grace E. Kissling, Daven N. Jackson-Humbles, Keith R. Shockley, and Scott S. Auerbach
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0301 basic medicine ,Male ,medicine.medical_specialty ,Phagocytosis ,Spleen ,Toxicology ,medicine.disease_cause ,Dermatitis, Contact ,Article ,Rats, Sprague-Dawley ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Sensitization ,Carcinogen ,Mice, Inbred BALB C ,Aniline Compounds ,Micronucleus Tests ,Dose-Response Relationship, Drug ,Chemistry ,Mutagenicity Tests ,Reproduction ,Body Weight ,Organ Size ,Nitro Compounds ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Liver ,Hemosiderin ,Gestation ,Female ,Comet Assay ,Lymph Nodes ,Reproductive toxicity ,030217 neurology & neurosurgery ,Genotoxicity - Abstract
2-Methoxy-4-nitroaniline (MNA), an intermediate in the synthesis of azo dyes used in textiles and paints, is structurally similar to carcinogenic anilines. Human exposure occurs primarily in the occupational setting through handling of dye dust, and through use and disposal of MNA-containing products. MNA has been reported to induce contact hypersensitivity in a human, myocardial necrosis in rats, and bacterial mutagenicity. This study assessed the subacute toxicity, genotoxicity, contact hypersensitivity, and reproductive toxicity of MNA in rodents in an effort to more fully characterize its toxicological profile. B6C3F1/N mice were exposed to 0, 650, 1250, 2500, 5000, or 10,000 ppm MNA by dosed feed for 14-days to evaluate subacute toxicity and histopathological endpoints. In female mice, decreased body weight (13.5 %) and absolute kidney weight (14.8 %), compared to control, were observed at 10,000 ppm MNA; increased relative liver weight (10-12 %), compared to control, occurred at 5,000-10,000 ppm MNA. In male mice, absolute (15 %) and relative liver weights (9-13 %) were increased at 2,500-5,000 ppm and 1250-10,000 ppm MNA, compared to control, respectively. In both sexes of mice, minimal elevations of hemosiderin pigmentation (a breakdown product of erythrocytes), relative to control, were observed in the liver (10,000 ppm); minimal to moderate elevations of hemosiderin pigmentation (5,000-10,000 ppm) and minimal increases in hematopoietic cell proliferation occurred in the spleen (≥ 1250 ppm). In a reproductive toxicity study, timed-mated female Harlan Sprague Dawley rats were exposed to 0-10,000 ppm MNA by dosed feed from gestation day 6 through postnatal day (PND) 21. Decreases in mean litter weights were observed at 5000 ppm MNA, compared to control, beginning at PND1. To evaluate potential contact hypersensitivity, MNA (2.5-50 %, in dimethylformamide) was applied to the dorsa of both ears of female Balb/c mice once daily for three days. The increase observed in lymph node cell proliferation (10-50 % increase in thymidine uptake compared to control) did not reproducibly achieve the Sensitization Index (SI) 3 level, and there was no ear swelling evident following sensitization with 10-50 % MNA and challenge with 25 % MNA in the mouse ear swelling test. In bacterial mutagenicity assays, MNA (250-1000 μg/plate) induced significant increases, compared to control, in mutant colonies with and without metabolic activation enzymes in Salmonella typhimurium strains TA100 and TA98. These data indicate that MNA is genotoxic, and may induce erythrocyte damage and reactive phagocytosis by macrophages in the liver and spleen.
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- 2020
48. Combination of urea-crosslinked hyaluronic acid and sodium ascorbyl phosphate for the treatment of inflammatory lung diseases: An in vitro study
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Hui Xin Ong, Daniela Traini, Paul M. Young, Laura Busato, Arianna Fallacara, Michele Pozzoli, Maliheh Ghadiri, and Stefano Manfredini
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0301 basic medicine ,Antioxidant ,genetic structures ,medicine.medical_treatment ,Anti-Inflammatory Agents ,Pharmaceutical Science ,Ascorbic Acid ,Pharmacology ,medicine.disease_cause ,Antioxidants ,Sodium ascorbyl phosphate ,chemistry.chemical_compound ,0302 clinical medicine ,Hyaluronic acid ,Electric Impedance ,Urea ,Lung ,chemistry.chemical_classification ,Viscosity ,Chemistry ,Biological activity ,Hydrogen-Ion Concentration ,Drug Combinations ,Cross-Linking Reagents ,Urea-crosslinked hyaluronic acid ,medicine.anatomical_structure ,medicine.symptom ,Cell Survival ,medicine.drug_class ,Drug Compounding ,Wound healing ,Inflammation ,Anti-inflammatory ,NO ,03 medical and health sciences ,Cell Line, Tumor ,medicine ,Humans ,Technology, Pharmaceutical ,Lung Diseases, Obstructive ,Reactive oxygen species ,Dose-Response Relationship, Drug ,Interleukin-6 ,Osmolar Concentration ,Epithelial Cells ,030104 developmental biology ,030228 respiratory system ,Reactive Oxygen Species ,Oxidative stress - Abstract
This in vitro study evaluated, for the first time, the safety and the biological activity of a novel urea-crosslinked hyaluronic acid component and sodium ascorbyl phosphate (HA-CL - SAP), singularly and/or in combination, intended for the treatment of inflammatory lung diseases. The aim was to understand if the combination HA-CL - SAP had an enhanced activity with respect to the combination native hyaluronic acid (HA) - SAP and the single SAP, HA and HA-CL components. Sample solutions displayed pH, osmolality and viscosity values suitable for lung delivery and showed to be not toxic on epithelial Calu-3 cells at the concentrations used in this study. The HA-CL - SAP displayed the most significant reduction in interleukin-6 (IL-6) and reactive oxygen species (ROS) levels, due to the combined action of HA-CL and SAP. Moreover, this combination showed improved cellular healing (wound closure) with respect to HA - SAP, SAP and HA, although at a lower rate than HA-CL alone. These preliminary results showed that the combination HA-CL - SAP could be suitable to reduce inflammation and oxidative stress in lung disorders like acute respiratory distress syndrome, asthma, emphysema and chronic obstructive pulmonary disease, where inflammation is prominent.
- Published
- 2018
49. The effects of the reorganisation of an intensive care unit due to COVID-19 on nurses’ wellbeing: An observational cross-sectional study
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Salvatore Pagliaro, Nunzio De Feo, Massimo Elisei, Monica Scateni, Antonio Fallacara, Francesco Forfori, and Nicola Pagnucci
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Male ,Cross-sectional study ,Nurses ,Context (language use) ,Critical Care Nursing ,Article ,Job Satisfaction ,law.invention ,Competence (law) ,03 medical and health sciences ,0302 clinical medicine ,Nursing ,law ,Surveys and Questionnaires ,Intensive care ,Humans ,Medicine ,Hospital reorganization ,Pandemic ,030504 nursing ,Wellbeing ,SARS-CoV-2 ,business.industry ,COVID-19 ,030208 emergency & critical care medicine ,Intensive care unit ,Work experience ,Intensive Care Units ,Cross-Sectional Studies ,Covid-19 ,Job satisfaction ,Female ,Observational study ,0305 other medical science ,business - Abstract
Objectives To evaluate the effects of the reorganisation of an intensive care unit for COVID-19 patients in the context of the SARS-CoV-2 pandemic on wellbeing perceived by nurses. Methods An observational cross-sectional study was conducted to evaluate wellbeing perceived by nurses who during the study were on duty in the COVID-19 intensive care unit. The “Covid-19-Nurse Well-being at Work (NWB) scale” questionnaire consisting of 72 items divided into 13 sections, was validated and used to collect data. Results The level of wellbeing perceived by the nurses was very good (4.77; SD 0.83). Differences in the of level of perceived wellbeing were found for “years of experience” and the various levels of competence. We found a positive correlation between “female gender” and “nurses’ togetherness and collaboration”, a negative correlation between “male gender” and “satisfactory practical organisation of work, and a negative correlation between “work experience” and the overall “level of wellbeing at work. Conclusions The reorganisation had positive effects in terms of wellbeing perceived by the nurses. The factors that contributed mostly to the perception of wellbeing were in the area of “support”, “communication, and “socializing with colleagues”. It is appropriate to consider “gender differences”, “work experience” and “levels of competence” when implementing this type of reorganisation to respond to a pandemic or a health emergency.
- Published
- 2021
50. Immunotoxicity studies of sulfolane following developmental exposure in Hsd:Sprague Dawley SD rats and adult exposure in B6C3F1/N mice
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Watson, AtLee T. D., primary, Johnson, Victor J., additional, Luster, Michael I., additional, Burleson, Gary R., additional, Fallacara, Dawn M., additional, Sparrow, Barney R., additional, Cesta, Mark F., additional, Cora, Michelle C., additional, Shockley, Keith R., additional, Stout, Matt D., additional, Blystone, Chad R., additional, and Germolec, Dori R., additional
- Published
- 2021
- Full Text
- View/download PDF
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