31 results on '"Cammarata, Francesco P."'
Search Results
2. Radioprotectors, Radiomitigators, and Radiosensitizers
- Author
-
Montoro, Alegría, Obrador, Elena, Mistry, Dhruti, Forte, Giusi I., Bravatà, Valentina, Minafra, Luigi, Calvaruso, Marco, Cammarata, Francesco P., Falk, Martin, Schettino, Giuseppe, Ahire, Vidhula, Daems, Noami, Boterberg, Tom, Dainiak, Nicholas, Chaudhary, Pankaj, Baatout, Sarah, Mishra, Kaushala Prasad, and Baatout, Sarah, editor
- Published
- 2023
- Full Text
- View/download PDF
3. Development of a portable hypoxia chamber for ultra-high dose rate laser-driven proton radiobiology applications
- Author
-
Chaudhary, Pankaj, Gwynne, Deborah C., Odlozilik, Boris, McMurray, Aaron, Milluzzo, Giuliana, Maiorino, Carla, Doria, Domenico, Ahmed, Hamad, Romagnani, Lorenzo, Alejo, Aaron, Padda, Hersimerjit, Green, James, Carroll, David, Booth, Nicola, McKenna, Paul, Kar, Satyabrata, Petringa, Giada, Catalano, Roberto, Cammarata, Francesco P., Cirrone, Giuseppe A. P., McMahon, Stephen J., Prise, Kevin M., and Borghesi, Marco
- Published
- 2022
- Full Text
- View/download PDF
4. Liver resection of hepatocellular carcinoma in HIV-HCV co-infected patients: a retrospective case series
- Author
-
Cammarata, Francesco, Benuzzi, Laura, Crespi, Michele, Troci, Albert, Pennacchi, Luca, Schiavini, Monica, and Foschi, Diego
- Published
- 2022
- Full Text
- View/download PDF
5. Glut-3 Gene Knockdown as a Potential Strategy to Overcome Glioblastoma Radioresistance
- Author
-
Pucci, Gaia, primary, Minafra, Luigi, additional, Bravatà, Valentina, additional, Calvaruso, Marco, additional, Turturici, Giuseppina, additional, Cammarata, Francesco P., additional, Savoca, Gaetano, additional, Abbate, Boris, additional, Russo, Giorgio, additional, Cavalieri, Vincenzo, additional, and Forte, Giusi I., additional
- Published
- 2024
- Full Text
- View/download PDF
6. Microglia and glioblastoma heterocellular interplay sustains tumour growth and proliferation as an off‐target effect of radiotherapy.
- Author
-
Alberghina, Cristiana, Torrisi, Filippo, D'Aprile, Simona, Longhitano, Lucia, Giallongo, Sebastiano, Scandura, Grazia, Mannino, Giuliana, Mele, Stefania, Sabini, Maria Gabriella, Cammarata, Francesco P., Russo, Giorgio, Abdelhameed, Ali S., Zappalà, Agata, Lo Furno, Debora, Giuffrida, Rosario, Li Volti, Giovanni, Tibullo, Daniele, Vicario, Nunzio, and Parenti, Rosalba
- Subjects
MICROGLIA ,BRAIN tumors ,GLIOBLASTOMA multiforme ,TUMOR microenvironment ,OXIDATIVE phosphorylation - Abstract
Glioblastoma (GBM), a WHO grade IV glioma, is a malignant primary brain tumour for which combination of surgery, chemotherapy and radiotherapy is the first‐line approach despite adverse effects. Tumour microenvironment (TME) is characterized by an interplay of cells and soluble factors holding a critical role in neoplastic development. Significant pathophysiological changes have been found in GBM TME, such as glia activation and oxidative stress. Microglia play a crucial role in favouring GBM growth, representing target cells of immune escape mechanisms. Our study aims at analysing radiation‐induced effects in modulating intercellular communication and identifying the basis of protective mechanisms in radiation‐naïve GBM cells. Tumour cells were treated with conditioned media (CM) derived from 0, 2 or 15 Gy irradiated GBM cells or 0, 2 or 15 Gy irradiated human microglia. We demonstrated that irradiated microglia promote an increase of GBM cell lines proliferation through paracrine signalling. On the contrary, irradiated GBM‐derived CM affect viability, triggering cell death mechanisms. In addition, we investigated whether these processes involve mitochondrial mass, fitness and oxidative phosphorylation and how GBM cells respond at these induced alterations. Our study suggests that off‐target radiotherapy modulates microglia to support GBM proliferation and induce metabolic modifications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. Radiosensitizing effect of curcumin-loaded lipid nanoparticles in breast cancer cells
- Author
-
Minafra, Luigi, Porcino, Nunziatina, Bravatà, Valentina, Gaglio, Daniela, Bonanomi, Marcella, Amore, Erika, Cammarata, Francesco Paolo, Russo, Giorgio, Militello, Carmelo, Savoca, Gaetano, Baglio, Margherita, Abbate, Boris, Iacoviello, Giuseppina, Evangelista, Giovanna, Gilardi, Maria Carla, Bondì, Maria Luisa, and Forte, Giusi Irma
- Published
- 2019
- Full Text
- View/download PDF
8. Additional file 1 of Development of a portable hypoxia chamber for ultra-high dose rate laser-driven proton radiobiology applications
- Author
-
Chaudhary, Pankaj, Gwynne, Deborah C., Odlozilik, Boris, McMurray, Aaron, Milluzzo, Giuliana, Maiorino, Carla, Doria, Domenico, Ahmed, Hamad, Romagnani, Lorenzo, Alejo, Aaron, Padda, Hersimerjit, Green, James, Carroll, David, Booth, Nicola, McKenna, Paul, Kar, Satyabrata, Petringa, Giada, Catalano, Roberto, Cammarata, Francesco P., Cirrone, Giuseppe A. P., McMahon, Stephen J., Prise, Kevin M., and Borghesi, Marco
- Subjects
ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,ComputingMilieux_COMPUTERSANDEDUCATION ,Data_FILES ,ComputerApplications_COMPUTERSINOTHERSYSTEMS - Abstract
Additional file 1. Supplementary tables and figures.
- Published
- 2022
- Full Text
- View/download PDF
9. The Hallmarks of Glioblastoma: Heterogeneity, Intercellular Crosstalk and Molecular Signature of Invasiveness and Progression
- Author
-
Torrisi, Filippo, primary, Alberghina, Cristiana, additional, D’Aprile, Simona, additional, Pavone, Anna M., additional, Longhitano, Lucia, additional, Giallongo, Sebastiano, additional, Tibullo, Daniele, additional, Di Rosa, Michelino, additional, Zappalà, Agata, additional, Cammarata, Francesco P., additional, Russo, Giorgio, additional, Ippolito, Massimo, additional, Cuttone, Giacomo, additional, Li Volti, Giovanni, additional, Vicario, Nunzio, additional, and Parenti, Rosalba, additional
- Published
- 2022
- Full Text
- View/download PDF
10. The Role of Radiation in Cancer Treatment: New Insights towards Personalized Therapies
- Author
-
Minafra, Luigi, primary, Cammarata, Francesco P., additional, and Calvaruso, Marco, additional
- Published
- 2022
- Full Text
- View/download PDF
11. Biological and Mechanical Characterization of the Random Positioning Machine (RPM) for Microgravity Simulations
- Author
-
Calvaruso, Marco, primary, Militello, Carmelo, additional, Minafra, Luigi, additional, La Regina, Veronica, additional, Torrisi, Filippo, additional, Pucci, Gaia, additional, Cammarata, Francesco P., additional, Bravatà, Valentina, additional, Forte, Giusi I., additional, and Russo, Giorgio, additional
- Published
- 2021
- Full Text
- View/download PDF
12. Transcriptional modulations induced by proton irradiation in mice skin in function of adsorbed dose and distance
- Author
-
Licursi, Valerio, Wang, Wei, Di Nisio, Elena, Cammarata, Francesco P., Acquaviva, Rosaria, Russo, Giorgio, Manti, Lorenzo, Guidi, Mariangela Cestelli, Fratini, Emiliano, Kamel, Gihan, Amendola, Roberto, Pisciotta, Pietro, and Negri, Rodolfo
- Abstract
Hadron therapy by proton beams represents an advanced anti-cancer strategy due to its highly localized dose deposition allowing a greater sparing of normal tissue and/or organs at risk compared to photon/electron radiotherapy. However, it is not clear to what extent non-targeted effects such as transcriptional modulations produced along the beamline may diffuse and impact the surrounding tissue. In this work, we analyze the transcriptome of proton-irradiated mouse skin and choose two biomarker genes to trace their modulation at different distances from the beam���s target and at different doses and times from irradiation to understand to what extent and how far it may propagate, using RNA-Seq and quantitative RT-PCR. In parallel, assessment of lipids alteration is performed by FTIR spectroscopy as a measure of tissue damage. Despite the observed high individual variability of expression, we can show evidence of transcriptional modulation of two biomarker genes at considerable distance from the beam���s target where a simulation system predicts a significantly lower adsorbed dose. The results are compatible with a model involving diffusion of transcripts or regulatory molecules from high dose irradiated cells to distant tissue���s portions adsorbing a much lower fraction of radiation.
- Published
- 2021
- Full Text
- View/download PDF
13. Hypoxia Transcriptomic Modifications Induced by Proton Irradiation in U87 Glioblastoma Multiforme Cell Line
- Author
-
Bravatà, Valentina, primary, Tinganelli, Walter, additional, Cammarata, Francesco P., additional, Minafra, Luigi, additional, Calvaruso, Marco, additional, Sokol, Olga, additional, Petringa, Giada, additional, Cirrone, Giuseppe A.P., additional, Scifoni, Emanuele, additional, Forte, Giusi I., additional, and Russo, Giorgio, additional
- Published
- 2021
- Full Text
- View/download PDF
14. The Role of Hypoxia and SRC Tyrosine Kinase in Glioblastoma Invasiveness and Radioresistance
- Author
-
Torrisi, Filippo, primary, Vicario, Nunzio, additional, Spitale, Federica M., additional, Cammarata, Francesco P., additional, Minafra, Luigi, additional, Salvatorelli, Lucia, additional, Russo, Giorgio, additional, Cuttone, Giacomo, additional, Valable, Samuel, additional, Gulino, Rosario, additional, Magro, Gaetano, additional, and Parenti, Rosalba, additional
- Published
- 2020
- Full Text
- View/download PDF
15. Molecular Investigation on a Triple Negative Breast Cancer Xenograft Model Exposed to Proton Beams
- Author
-
Cammarata, Francesco P., primary, Forte, Giusi I., additional, Broggi, Giuseppe, additional, Bravatà, Valentina, additional, Minafra, Luigi, additional, Pisciotta, Pietro, additional, Calvaruso, Marco, additional, Tringali, Roberta, additional, Tomasello, Barbara, additional, Torrisi, Filippo, additional, Petringa, Giada, additional, Cirrone, Giuseppe A. P., additional, Cuttone, Giacomo, additional, Acquaviva, Rosaria, additional, Caltabiano, Rosario, additional, and Russo, Giorgio, additional
- Published
- 2020
- Full Text
- View/download PDF
16. SRC Tyrosine Kinase Inhibitor and X-rays Combined Effect on Glioblastoma Cell Lines
- Author
-
Torrisi, Filippo, primary, Minafra, Luigi, additional, Cammarata, Francesco P., additional, Savoca, Gaetano, additional, Calvaruso, Marco, additional, Vicario, Nunzio, additional, Maccari, Laura, additional, Pérès, Elodie A., additional, Özçelik, Hayriye, additional, Bernaudin, Myriam, additional, Botta, Lorenzo, additional, Russo, Giorgio, additional, Parenti, Rosalba, additional, and Valable, Samuel, additional
- Published
- 2020
- Full Text
- View/download PDF
17. Nutraceutical Compounds as Sensitizers for Cancer Treatment in Radiation Therapy
- Author
-
Calvaruso, Marco, primary, Pucci, Gaia, additional, Musso, Rosa, additional, Bravatà, Valentina, additional, Cammarata, Francesco P., additional, Russo, Giorgio, additional, Forte, Giusi I., additional, and Minafra, Luigi, additional
- Published
- 2019
- Full Text
- View/download PDF
18. Proton Therapy and Src Family Kinase Inhibitor Combined Treatments on U87 Human Glioblastoma Multiforme Cell Line
- Author
-
Cammarata, Francesco P, primary, Torrisi, Filippo, additional, Forte, Giusi I, additional, Minafra, Luigi, additional, Bravatà, Valentina, additional, Pisciotta, Pietro, additional, Savoca, Gaetano, additional, Calvaruso, Marco, additional, Petringa, Giada, additional, Cirrone, Giuseppe AP, additional, Fallacara, Anna L, additional, Maccari, Laura, additional, Botta, Maurizio, additional, Schenone, Silvia, additional, Parenti, Rosalba, additional, Cuttone, Giacomo, additional, and Russo, Giorgio, additional
- Published
- 2019
- Full Text
- View/download PDF
19. Proton-irradiated breast cells: molecular points of view
- Author
-
Bravatà, Valentina, primary, Cammarata, Francesco P, additional, Minafra, Luigi, additional, Pisciotta, Pietro, additional, Scazzone, Concetta, additional, Manti, Lorenzo, additional, Savoca, Gaetano, additional, Petringa, Giada, additional, Cirrone, Giuseppe A P, additional, Cuttone, Giacomo, additional, Gilardi, Maria C, additional, Forte, Giusi I, additional, and Russo, Giorgio, additional
- Published
- 2019
- Full Text
- View/download PDF
20. DVWA gene polymorphisms and osteoarthritis
- Author
-
Bravatà, Valentina, primary, Minafra, Luigi, additional, Forte, Giusi I, additional, Cammarata, Francesco P, additional, Saporito, Michele, additional, Boniforti, Filippo, additional, Lio, Domenico, additional, Gilardi, Maria C, additional, and Messa, Cristina, additional
- Published
- 2015
- Full Text
- View/download PDF
21. Genetic, clinical and radiographic signs in knee osteoarthritis susceptibility
- Author
-
Minafra, Luigi, primary, Bravatà, Valentina, additional, Saporito, Michele, additional, Cammarata, Francesco P, additional, Forte, Giusi I, additional, Caldarella, Salvatore, additional, D’Arienzo, Michele, additional, Gilardi, Maria C, additional, Messa, Cristina, additional, and Boniforti, Filippo, additional
- Published
- 2014
- Full Text
- View/download PDF
22. Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes
- Author
-
Camarda, Massimo, primary, Fisicaro, Giuseppe, additional, Anzalone, Ruggero, additional, Scalese, Silvia, additional, Alberti, Alessandra, additional, La Via, Francesco, additional, La Magna, Antonino, additional, Ballo, Andrea, additional, Giustolisi, Gianluca, additional, Minafra, Luigi, additional, Cammarata, Francesco P, additional, Bravatà, Valentina, additional, Forte, Giusi I, additional, Russo, Giorgio, additional, and Gilardi, Maria C, additional
- Published
- 2014
- Full Text
- View/download PDF
23. Genotyping analysis and 18FDG uptake in breast cancer patients: a preliminary research
- Author
-
Bravatà, Valentina, primary, Stefano, Alessandro, additional, Cammarata, Francesco P, additional, Minafra, Luigi, additional, Russo, Giorgio, additional, Nicolosi, Stefania, additional, Pulizzi, Sabina, additional, Gelfi, Cecilia, additional, Gilardi, Maria C, additional, and Messa, Cristina, additional
- Published
- 2013
- Full Text
- View/download PDF
24. Genotyping analysis and 18FDG uptake in breast cancer patients: a preliminary research.
- Author
-
Bravatà, Valentina, Stefano, Alessandro, Cammarata, Francesco P., Minafra, Luigi, Russo, Giorgio, Nicolosi, Stefania, Pulizzi, Sabina, Gelfi, Cecilia, Gilardi, Maria C., and Messa, Cristina
- Subjects
BREAST cancer ,POSITRON emission tomography ,BREAST cancer patients ,QUANTITATIVE research ,GENETIC polymorphisms ,SINGLE nucleotide polymorphisms ,NUCLEOTIDE sequence - Abstract
Background: Diagnostic imaging plays a relevant role in the care of patients with breast cancer (BC). Positron Emission Tomography (PET) with 18F-fluoro-2-deoxy-D-glucose (FDG) has been widely proven to be a clinical tool suitable for BC detection and staging in which the glucose analog supplies metabolic information about the tumor. A limited number of studies, sometimes controversial, describe possible associations between FDG uptake and single nucleotide polymorphisms (SNPs). For this reason this field has to be explored and clarified. We investigated the association of SNPs in GLUT1, HIF-1a, EPAS1, APEX1, VEGFA and MTHFR genes with the FDG uptake in BC. Methods: In 26 caucasian individuals with primary BC, whole-body PET-CT scans were obtained and quantitative analysis was performed by calculating the maximum Standardized Uptake Value normalized to body-weight (SUVmax) and the mean SUV normalized to body-weight corrected for partial volume effect (SUVpvc). Human Gene Mutation Database and dbSNP Short Genetic Variations database were used to analyze gene regions containing the selected SNPs. Patient genotypes were obtained using Sanger DNA sequencing analysis performed by Capillary Electrophoresis. Results: BC patients were genotyped for the following nine SNPs: GLUT1: rs841853 and rs710218; HIF-1a: rs11549465 and rs11549467; EPAS1: rs137853037 and rs137853036; APEX1: rs1130409; VEGFA: rs3025039 and MTHFR: rs1801133. In this work correlations between the nine potentially useful polymorphisms selected and previously suggested with tracer uptake (using both SUVmax and SUVpvc) were not found. Conclusions: The possible functional influence of specific SNPs on FDG uptake needs further studies in human cancer. In summary, this is the first pilot study, to our knowledge, which investigates the association between a large panel of SNPs and FDG uptake specifically in BC patients. This work represents a multidisciplinary and translational medicine approach to study BC where, the possible correlation between SNPs and tracer uptake, may be considered to improve personalized cancer treatment and care. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
25. Future Perspectives of Proton Therapy in Minimizing the Toxicity of Breast Cancer Radiotherapy.
- Author
-
Musielak, Marika, Suchorska, Wiktoria M., Fundowicz, Magdalena, Milecki, Piotr, Malicki, Julian, Minafra, Luigi, Cammarata, Francesco P., and Calvaruso, Marco
- Subjects
PROTON therapy ,CANCER radiotherapy ,BREAST cancer ,CARDIOTOXICITY ,BLOOD vessels ,PHOTON beams - Abstract
The toxicity of radiotherapy is a key issue when analyzing the eligibility criteria for patients with breast cancer. In order to obtain better results, proton therapy is proposed because of the more favorable distribution of the dose in the patient's body compared with photon radiotherapy. Scientific groups have conducted extensive research into the improved efficacy and lower toxicity of proton therapy for breast cancer. Unfortunately, there is no complete insight into the potential reasons and prospects for avoiding undesirable results. Cardiotoxicity is considered challenging; however, researchers have not presented any realistic prospects for preventing them. We compared the clinical evidence collected over the last 20 years, providing the rationale for the consideration of proton therapy as an effective solution to reduce cardiotoxicity. We analyzed the parameters of the dose distribution (mean dose, Dmax, V5, and V20) in organs at risk, such as the heart, blood vessels, and lungs, using the following two irradiation techniques: whole breast irradiation and accelerated partial breast irradiation. Moreover, we presented the possible causes of side effects, taking into account biological and technical issues. Finally, we collected potential improvements in higher quality predictions of toxic cardiac effects, like biomarkers, and model-based approaches to give the full background of this complex issue. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
26. Sensitization of chondrosarcoma cells with PARP inhibitor and high-LET radiation.
- Author
-
Césaire, Mathieu, Ghosh, Utpal, Austry, Jean-Baptiste, Muller, Etienne, Cammarata, Francesco Paolo, Guillamin, Marilyne, Caruso, Massimo, Castéra, Laurent, Petringa, Giada, Cirrone, Giuseppe Antonio Pablo, and Chevalier, François
- Abstract
Chondrosarcoma is a malignant tumor that arises from cartilaginous tissue and is radioresistant and chemoresistant to conventional treatments. The preferred treatment consists of surgical resection, which might cause severe disabilities for the patient; in addition, this procedure might be impossible for inoperable locations, such as the skull base. Carbon ion irradiation (hadron therapy) has been proposed as an alternative treatment, primarily due to its greater biological effectiveness and improved ballistic properties compared with conventional radiotherapy with X-rays. The goal of this study was to characterize the genetic mutations of a grade III chondrosarcoma cell line (CH2879) and examine the cellular responses to conventional radiotherapy (X-rays) and hadron therapy (proton and carbon ions) in the presence of the PARP inhibitor Olaparib. To better understand PARP inhibition, we first analyzed the formation of poly-ADP ribose chains by western blot; we observed an increase in its signal after irradiation, which disappeared on addition of the PARP inhibitor. PARPi enhanced ratio of approximately 1.3, 1.8, and 1.5 following irradiation of cells with X-rays, protons, and C-ions, respectively, as detected by clonogenic assay. The decrease in cell survival was confirmed by proliferation assay. The radiosensitivity of CH2879 cells was associated with mutations in homologous recombination repair genes, such as RAD50, SMARCA2 and NBN. This study demonstrates the capacity of the PARP inhibitor Olaparib to radiosensitize mutated chondrosarcoma cells to conventional photon irradiation, proton and carbon ion irradiation. Image, graphical abstract [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
27. Transcriptional modulations induced by proton irradiation in mice skin in function of adsorbed dose and distance
- Author
-
Emiliano Fratini, Gihan Kamel, Giorgio Ivan Russo, Rosaria Acquaviva, Elena Di Nisio, Wei Wang, Francesco Paolo Cammarata, Roberto Amendola, Valerio Licursi, Lorenzo Manti, Mariangela Cestelli Guidi, Pietro Pisciotta, Rodolfo Negri, Licursi, Valerio, Wang, Wei, Di Nisio, Elena, Cammarata, Francesco P., Acquaviva, Rosaria, Russo, Giorgio, Manti, Lorenzo, Cestelli Guidi, Mariangela, Fratini, Emiliano, Kamel, Gihan, Amendola, Roberto, Pisciotta, Pietro, and Negri, Rodolfo
- Subjects
Proton irradiation ,handrontherapy ,transcriptome modulation ,mouse skin ,spectroscopy FTIR microspectroscopy ,DIFFERENTIAL GENE-EXPRESSION ,integumentary system ,Proton ,IDENTIFICATION ,Chemistry ,Normal tissue ,ACUTE EXPOSURE ,RADIATION-INDUCED DAMAGE ,Ionizing radiation ,Hadron therapy ,Acute exposure ,Mouse skin ,CELLS ,Biophysics ,Irradiation ,IONIZING-RADIATION ,Function (biology) ,Proton irradiation, handrontherapy, transcriptome modulation, mouse skin, spectroscopy, FTIR microspectroscopy - Abstract
Hadron therapy by proton beams represents an advanced anti-cancer strategy due to its highly localized dose deposition allowing a greater sparing of normal tissue and/or organs at risk compared to photon/electron radiotherapy. However, it is not clear to what extent non-targeted effects such as transcriptional modulations produced along the beamline may diffuse and impact the surrounding tissue. In this work, we analyze the transcriptome of proton-irradiated mouse skin and choose two biomarker genes to trace their modulation at different distances from the beam's target and at different doses and times from irradiation to understand to what extent and how far it may propagate, using RNA-Seq and quantitative RT-PCR. In parallel, assessment of lipids alteration is performed by FTIR spectroscopy as a measure of tissue damage. Despite the observed high individual variability of expression, we can show evidence of transcriptional modulation of two biomarker genes at considerable distance from the beam's target where a simulation system predicts a significantly lower adsorbed dose. The results are compatible with a model involving diffusion of transcripts or regulatory molecules from high dose irradiated cells to distant tissue's portions adsorbing a much lower fraction of radiation.
- Published
- 2021
28. Proton-irradiated breast cells: molecular points of view
- Author
-
Valentina Bravatà 1, Francesco P. Cammarata 1, Luigi Minafra 1, Pietro Pisciotta 1, 2, Concetta Scazzone 3, Lorenzo Manti 4, Gaetano Savoca 1, Giada Petringa 1, Giuseppe A.P. Cirrone 2, Giacomo Cuttone 2, Maria C. Gilardi 1, 5, Giusi I. Forte 1, Giorgio Russo 1, Bravata, V, Cammarata, F, Minafra, L, Pisciotta, P, Scazzone, C, Manti, L, Savoca, G, Petringa, G, Cirrone, G, Cuttone, G, Gilardi, M, Forte, G, Russo, G, Bravata, V., Cammarata, F. P., Minafra, L., Pisciotta, P., Scazzone, C., Manti, L., Savoca, G., Petringa, G., Cirrone, G. A. P., Cuttone, G., Gilardi, M. C., Forte, G. I., Russo, G., Bravatà, Valentina, Cammarata, Francesco P, Minafra, Luigi, Pisciotta, Pietro, Scazzone, Concetta, Manti, Lorenzo, Savoca, Gaetano, Petringa, Giada, Cirrone, Giuseppe A P, Cuttone, Giacomo, Gilardi, Maria C, Forte, Giusi I, and Russo, Giorgio
- Subjects
breast cancer, cDNA microarray, gene signature, proton therapy, radiation, Breast, Breast Neoplasms, Cell Line, Tumor, DNA, Complementary, Dose-Response Relationship, Radiation, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Inflammation, MCF-7 Cells, Oligonucleotide Array Sequence Analysis, Phenotype, Proton Therapy, Radiation Tolerance, Radiotherapy, Protons ,DNA, Complementary ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,Breast Neoplasms ,Cell fate determination ,Radiation Tolerance ,gene signature ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,breast cancer ,Cell Line, Tumor ,Regular Paper ,medicine ,proton therapy ,Humans ,Radiology, Nuclear Medicine and imaging ,Breast ,Clonogenic assay ,Biology ,Proton therapy ,Oligonucleotide Array Sequence Analysis ,030304 developmental biology ,Inflammation ,cDNA microarray ,0303 health sciences ,Radiotherapy ,Chemistry ,Gene Expression Profiling ,radiation ,Cancer ,Dose-Response Relationship, Radiation ,Gene signature ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Gene expression profiling ,Radiation therapy ,Phenotype ,030220 oncology & carcinogenesis ,MCF-7 Cells ,Cancer research ,Female ,Protons - Abstract
Breast cancer (BC) is the most common cancer in women, highly heterogeneous at both the clinical and molecular level. Radiation therapy (RT) represents an efficient modality to treat localized tumor in BC care, although the choice of a unique treatment plan for all BC patients, including RT, may not be the best option. Technological advances in RT are evolving with the use of charged particle beams (i.e. protons) which, due to a more localized delivery of the radiation dose, reduce the dose administered to the heart compared with conventional RT. However, few data regarding proton-induced molecular changes are currently available. The aim of this study was to investigate and describe the production of immunological molecules and gene expression profiles induced by proton irradiation. We performed Luminex assay and cDNA microarray analyses to study the biological processes activated following irradiation with proton beams, both in the non-tumorigenic MCF10A cell line and in two tumorigenic BC cell lines, MCF7 and MDA-MB-231. The immunological signatures were dose dependent in MCF10A and MCF7 cell lines, whereas MDA-MB-231 cells show a strong pro-inflammatory profile regardless of the dose delivered. Clonogenic assay revealed different surviving fractions according to the breast cell lines analyzed. We found the involvement of genes related to cell response to proton irradiation and reported specific cell line- and dose-dependent gene signatures, able to drive cell fate after radiation exposure. Our data could represent a useful tool to better understand the molecular mechanisms elicited by proton irradiation and to predict treatment outcome
- Published
- 2019
29. Radiation Gene-expression Signatures in Primary Breast Cancer Cells.
- Author
-
Minafra L, Bravatà V, Cammarata FP, Russo G, Gilardi MC, and Forte GI
- Subjects
- Biomarkers, Tumor, Breast Neoplasms pathology, DNA, Complementary genetics, Dose-Response Relationship, Radiation, Female, Humans, Intraoperative Care, Radiation Tolerance, Real-Time Polymerase Chain Reaction, Tissue Array Analysis, Tumor Cells, Cultured, Whole Genome Sequencing, Breast Neoplasms genetics, Electrons therapeutic use, Gene Expression Regulation, Neoplastic radiation effects, Radiotherapy, High-Energy, Transcriptome
- Abstract
Background/aim: In breast cancer (BC) care, radiation therapy (RT) is an efficient treatment to control localized tumor. Radiobiological research is needed to understand molecular differences that affect radiosensitivity of different tumor subtypes and the response variability. The aim of this study was to analyze gene expression profiling (GEP) in primary BC cells following irradiation with doses of 9 Gy and 23 Gy delivered by intraoperative electron radiation therapy (IOERT) in order to define gene signatures of response to high doses of ionizing radiation., Materials and Methods: We performed GEP by cDNA microarrays and evaluated cell survival after IOERT treatment in primary BC cell cultures. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate candidate genes., Results: We showed, for the first time, a 4-gene and a 6-gene signature, as new molecular biomarkers, in two primary BC cell cultures after exposure at 9 Gy and 23 Gy respectively, for which we observed a significantly high survival rate., Conclusion: Gene signatures activated by different doses of ionizing radiation may predict response to RT and contribute to defining a personalized biological-driven treatment plan., (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
30. High-dose Ionizing Radiation Regulates Gene Expression Changes in the MCF7 Breast Cancer Cell Line.
- Author
-
Bravatà V, Minafra L, Russo G, Forte GI, Cammarata FP, Ripamonti M, Casarino C, Augello G, Costantini F, Barbieri G, Messa C, and Gilardi MC
- Subjects
- Breast Neoplasms pathology, Cellular Senescence genetics, Cellular Senescence radiation effects, Dose-Response Relationship, Radiation, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic radiation effects, Humans, MCF-7 Cells, Oligonucleotide Array Sequence Analysis, Breast Neoplasms genetics, Breast Neoplasms radiotherapy, Radiation Tolerance genetics, Radiation, Ionizing
- Abstract
Background: Intraoperative electron radiation therapy (IOERT) is a therapeutic technique which administers a single high dose of ionizing radiation immediately after surgical tumor removal. IOERT induces a strong stress response: both tumor and normal cells activating pro- and antiproliferative cell signaling pathways. Following treatment, several genes and factors are differently modulated, producing an imbalance in cell fate decision. However, the contribution of these genes and pathways in conferring different cell radiosensitivity and radioresistance needs to be further investigated, in particular after high-dose treatments. Despite the documented and great impact of IOERT in breast cancer care, and the trend for dose escalation, very limited data are available regarding gene-expression profiles and cell networks activated by IOERT or high-dose treatment. The aim of the study was to analyze the main pathways activated following high radiation doses in order to select for potential new biomarkers of radiosensitivity or radioresistance, as well as to identify therapeutic targets useful in cancer care., Materials and Methods: We performed gene-expression profiling of the MCF7 human breast carcinoma cell line after treatment with 9- and 23-Gy doses (conventionally used during IOERT boost and exclusive treatments, respectively) by cDNA microarrays. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR), immunofluorescence and immunoblot experiments were performed to validate candidate IOERT biomarkers. We also conducted clonogenic tests and cellular senescence assays to monitor for radiation-induced effects., Results: The analyses highlighted a transcriptome dependent on the dose delivered and a number of specific key genes that may be proposed as new markers of radiosensitivity. Cell and molecular traits observed in MCF7 cells revealed a typical senescent phenotype associated with cell proliferation arrest after treatments with 9- and 23-Gy doses., Conclusion: In this study, we report genes and cellular networks activated following high-dose IOERT. The selected validated genes were used to design two descriptive models for each dose delivered. We believe that this study could contribute to the understanding over the complex mechanisms which regulate cell radiosensitivity and radioresistance in order to improve personalized radiotherapeutic treatment., (Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Published
- 2015
31. Genotyping analysis and ¹⁸FDG uptake in breast cancer patients: a preliminary research.
- Author
-
Bravatà V, Stefano A, Cammarata FP, Minafra L, Russo G, Nicolosi S, Pulizzi S, Gelfi C, Gilardi MC, and Messa C
- Subjects
- Breast Neoplasms diagnostic imaging, Breast Neoplasms pathology, Female, Genotype, Humans, Multimodal Imaging, Polymorphism, Single Nucleotide, Positron-Emission Tomography, Radiopharmaceuticals pharmacokinetics, Tomography, X-Ray Computed, Breast Neoplasms genetics, Breast Neoplasms metabolism, Fluorodeoxyglucose F18 pharmacokinetics
- Abstract
Background: Diagnostic imaging plays a relevant role in the care of patients with breast cancer (BC). Positron Emission Tomography (PET) with 18F-fluoro-2-deoxy-D-glucose (FDG) has been widely proven to be a clinical tool suitable for BC detection and staging in which the glucose analog supplies metabolic information about the tumor. A limited number of studies, sometimes controversial, describe possible associations between FDG uptake and single nucleotide polymorphisms (SNPs). For this reason this field has to be explored and clarified. We investigated the association of SNPs in GLUT1, HIF-1a, EPAS1, APEX1, VEGFA and MTHFR genes with the FDG uptake in BC., Methods: In 26 caucasian individuals with primary BC, whole-body PET-CT scans were obtained and quantitative analysis was performed by calculating the maximum Standardized Uptake Value normalized to body-weight (SUVmax) and the mean SUV normalized to body-weight corrected for partial volume effect (SUVpvc). Human Gene Mutation Database and dbSNP Short Genetic Variations database were used to analyze gene regions containing the selected SNPs. Patient genotypes were obtained using Sanger DNA sequencing analysis performed by Capillary Electrophoresis., Results: BC patients were genotyped for the following nine SNPs: GLUT1: rs841853 and rs710218; HIF-1a: rs11549465 and rs11549467; EPAS1: rs137853037 and rs137853036; APEX1: rs1130409; VEGFA: rs3025039 and MTHFR: rs1801133. In this work correlations between the nine potentially useful polymorphisms selected and previously suggested with tracer uptake (using both SUVmax and SUVpvc) were not found., Conclusions: The possible functional influence of specific SNPs on FDG uptake needs further studies in human cancer. In summary, this is the first pilot study, to our knowledge, which investigates the association between a large panel of SNPs and FDG uptake specifically in BC patients. This work represents a multidisciplinary and translational medicine approach to study BC where, the possible correlation between SNPs and tracer uptake, may be considered to improve personalized cancer treatment and care.
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.