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Transcriptional modulations induced by proton irradiation in mice skin in function of adsorbed dose and distance
- Source :
- Journal of Radiation Research and Applied Sciences 14 (2021): 260–270. doi:10.1080/16878507.2021.1949675, info:cnr-pdr/source/autori:Licursi V.; Wang W.; Di Nisio E.; Cammarata F.P.; Acquaviva R.; Russo G.; Manti L.; Cestelli Guidi M.; Fratini E.; Kamel G.; Amendola R.; Pisciotta P.; Negri R./titolo:Transcriptional modulations induced by proton irradiation in mice skin in function of adsorbed dose and distance/doi:10.1080%2F16878507.2021.1949675/rivista:Journal of Radiation Research and Applied Sciences/anno:2021/pagina_da:260/pagina_a:270/intervallo_pagine:260–270/volume:14, Journal of radiation research and applied sciences, 14(1), 260-270. Taylor & Francis Ltd
- Publication Year :
- 2021
- Publisher :
- Egyptian Society of Radiation Sciences and Applications, Cairo, Egitto, 2021.
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Abstract
- Hadron therapy by proton beams represents an advanced anti-cancer strategy due to its highly localized dose deposition allowing a greater sparing of normal tissue and/or organs at risk compared to photon/electron radiotherapy. However, it is not clear to what extent non-targeted effects such as transcriptional modulations produced along the beamline may diffuse and impact the surrounding tissue. In this work, we analyze the transcriptome of proton-irradiated mouse skin and choose two biomarker genes to trace their modulation at different distances from the beam's target and at different doses and times from irradiation to understand to what extent and how far it may propagate, using RNA-Seq and quantitative RT-PCR. In parallel, assessment of lipids alteration is performed by FTIR spectroscopy as a measure of tissue damage. Despite the observed high individual variability of expression, we can show evidence of transcriptional modulation of two biomarker genes at considerable distance from the beam's target where a simulation system predicts a significantly lower adsorbed dose. The results are compatible with a model involving diffusion of transcripts or regulatory molecules from high dose irradiated cells to distant tissue's portions adsorbing a much lower fraction of radiation.
- Subjects :
- Proton irradiation
handrontherapy
transcriptome modulation
mouse skin
spectroscopy FTIR microspectroscopy
DIFFERENTIAL GENE-EXPRESSION
integumentary system
Proton
IDENTIFICATION
Chemistry
Normal tissue
ACUTE EXPOSURE
RADIATION-INDUCED DAMAGE
Ionizing radiation
Hadron therapy
Acute exposure
Mouse skin
CELLS
Biophysics
Irradiation
IONIZING-RADIATION
Function (biology)
Proton irradiation, handrontherapy, transcriptome modulation, mouse skin, spectroscopy, FTIR microspectroscopy
Subjects
Details
- Language :
- English
- ISSN :
- 16878507
- Database :
- OpenAIRE
- Journal :
- Journal of Radiation Research and Applied Sciences 14 (2021): 260–270. doi:10.1080/16878507.2021.1949675, info:cnr-pdr/source/autori:Licursi V.; Wang W.; Di Nisio E.; Cammarata F.P.; Acquaviva R.; Russo G.; Manti L.; Cestelli Guidi M.; Fratini E.; Kamel G.; Amendola R.; Pisciotta P.; Negri R./titolo:Transcriptional modulations induced by proton irradiation in mice skin in function of adsorbed dose and distance/doi:10.1080%2F16878507.2021.1949675/rivista:Journal of Radiation Research and Applied Sciences/anno:2021/pagina_da:260/pagina_a:270/intervallo_pagine:260–270/volume:14, Journal of radiation research and applied sciences, 14(1), 260-270. Taylor & Francis Ltd
- Accession number :
- edsair.doi.dedup.....97c6c88a15d6ee5b238c6fe9fd5b9197