33 results on '"Bhuiyan, Taufiqur R."'
Search Results
2. Association between chlorine-treated drinking water, the gut microbiome, and enteric pathogen burden in young children in Haiti: An observational study
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Chac, Denise, Slater, Damien M., Guillaume, Yodeline, Dunmire, Chelsea N., Ternier, Ralph, Vissières, Kenia, Juin, Stanley, Lucien, Mentor Ali Ber, Boncy, Jacques, Sanchez, Vanessa M., Dumayas, Mia G., Augustin, Gertrude Cene, Bhuiyan, Taufiqur R., Qadri, Firdausi, Chowdhury, Fahima, Khan, Ashraful I., Weil, Ana A., Ivers, Louise C., and Harris, Jason B.
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- 2024
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3. Predicting Vibrio cholerae infection and symptomatic disease: a systems serology study
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Wiens, Kirsten E, Iyer, Anita S, Bhuiyan, Taufiqur R, Lu, Lenette L, Cizmeci, Deniz, Gorman, Matthew J, Yuan, Dansu, Becker, Rachel L, Ryan, Edward T, Calderwood, Stephen B, LaRocque, Regina C, Chowdhury, Fahima, Khan, Ashraful I, Levine, Myron M, Chen, Wilbur H, Charles, Richelle C, Azman, Andrew S, Qadri, Firdausi, Alter, Galit, and Harris, Jason B
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- 2023
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4. Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells
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Kauffman, Robert C, Bhuiyan, Taufiqur R, Nakajima, Rie, Mayo-Smith, Leslie M, Rashu, Rasheduzzaman, Hoq, Mohammad Rubel, Chowdhury, Fahima, Khan, Ashraful Islam, Rahman, Atiqur, Bhaumik, Siddhartha K, Harris, Levelle, O'Neal, Justin T, Trost, Jessica F, Alam, Nur Haq, Jasinskas, Algis, Dotsey, Emmanuel, Kelly, Meagan, Charles, Richelle C, Xu, Peng, Kováč, Pavol, Calderwood, Stephen B, Ryan, Edward T, Felgner, Phillip L, Qadri, Firdausi, Wrammert, Jens, Harris, Jason B, Petri, William, Kaper, James, and Sack, David
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Biodefense ,Clinical Research ,Vaccine Related ,Foodborne Illness ,Prevention ,Biotechnology ,Immunization ,Emerging Infectious Diseases ,Infectious Diseases ,Digestive Diseases ,Infection ,Good Health and Well Being ,Adult ,Antibodies ,Bacterial ,Antibodies ,Monoclonal ,Antibody Formation ,B-Lymphocytes ,Bacterial Toxins ,Cholera ,Cholera Toxin ,Cross Reactions ,Enterotoxins ,Escherichia coli Proteins ,Humans ,Immunologic Memory ,Lipopolysaccharides ,Neuraminidase ,O Antigens ,Proteomics ,Serogroup ,Single-Cell Analysis ,Vibrio cholerae ,Microbiology - Abstract
We characterized the acute B cell response in adults with cholera by analyzing the repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. We found that the cholera-induced responses were characterized by high levels of somatic hypermutation and large clonal expansions. A majority of the expansions targeted cholera toxin (CT) or lipopolysaccharide (LPS). Using a novel proteomics approach, we were able to identify sialidase as another major antigen targeted by the antibody response to Vibrio cholerae infection. Antitoxin MAbs targeted both the A and B subunits, and most were also potent neutralizers of enterotoxigenic Escherichia coli heat-labile toxin. LPS-specific MAbs uniformly targeted the O-specific polysaccharide, with no detectable responses to either the core or the lipid moiety of LPS. Interestingly, the LPS-specific antibodies varied widely in serotype specificity and functional characteristics. One participant infected with the Ogawa serotype produced highly mutated LPS-specific antibodies that preferentially bound the previously circulating Inaba serotype. This demonstrates durable memory against a polysaccharide antigen presented at the mucosal surface and provides a mechanism for the long-term, partial heterotypic immunity seen following cholera.ImportanceCholera is a diarrheal disease that results in significant mortality. While oral cholera vaccines are beneficial, they do not achieve equivalent protection compared to infection with Vibrio cholerae Although antibodies likely mediate protection, the mechanisms of immunity following cholera are poorly understood, and a detailed understanding of antibody responses to cholera is of significance for human health. In this study, we characterized the human response to cholera at the single-plasmablast, monoclonal antibody level. Although this approach has not been widely applied to the study of human bacterial infection, we were able to uncover the basis of cross-reactivity between different V. cholerae serotypes and the likely impact of prior enterotoxigenic Escherichia coli exposure on the response to cholera, as well as identify novel antigenic targets. In addition to improving our understanding of the repertoire and function of the antibody response to cholera in humans, this study has implications for future cholera vaccination efforts.
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- 2016
5. Comparison of O-specific polysaccharide responses in patients following infection with Vibrio cholerae O139 versus vaccination with a bivalent (O1/O139) oral killed cholera vaccine in Bangladesh
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Kaisar, M. Hasanul, primary, Kelly, Meagan, additional, Kamruzzaman, Mohammad, additional, Bhuiyan, Taufiqur R., additional, Chowdhury, Fahima, additional, Khan, Ashraful Islam, additional, LaRocque, Regina C., additional, Calderwood, Stephen B., additional, Harris, Jason B., additional, Charles, Richelle C., additional, Čížová, Alžbeta, additional, Mečárová, Jana, additional, Korcová, Jana, additional, Bystrický, Slavomír, additional, Kováč, Pavol, additional, Xu, Peng, additional, Qadri, Firdausi, additional, and Ryan, Edward T., additional
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- 2023
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6. Identifying Recent Cholera Infections Using a Multiplex Bead Serological Assay
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Jones, Forrest K., primary, Bhuiyan, Taufiqur R., additional, Muise, Rachel E., additional, Khan, Ashraful I., additional, Slater, Damien M., additional, Hutt Vater, Kian Robert, additional, Chowdhury, Fahima, additional, Kelly, Meagan, additional, Xu, Peng, additional, Kováč, Pavol, additional, Biswas, Rajib, additional, Kamruzzaman, Mohammad, additional, Ryan, Edward T., additional, Calderwood, Stephen B., additional, LaRocque, Regina C., additional, Lessler, Justin, additional, Charles, Richelle C., additional, Leung, Daniel T., additional, Qadri, Firdausi, additional, Harris, Jason B., additional, and Azman, Andrew S., additional
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- 2022
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7. Vibrio cholerae Isolation from Frozen Vomitus and Stool Samples
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Dunmire, Chelsea N., primary, Chac, Denise, additional, Chowdhury, Fahima, additional, Khan, Ashraful I., additional, Bhuiyan, Taufiqur R., additional, LaRocque, Regina C., additional, Akter, Afroza, additional, Amin, Mohammad Ashraful, additional, Ryan, Edward T., additional, Qadri, Firdausi, additional, and Weil, Ana A., additional
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- 2022
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8. Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report
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Bhuiyan, Taufiqur R., primary, Rahman, M. Arifur, additional, Trivedi, Shubhanshi, additional, Afroz, Taliman, additional, Al Banna, Hasan, additional, Hoq, Mohammad Rubel, additional, Pop, Ioana, additional, Jensen, Owen, additional, Rashu, Rasheduzzaman, additional, Uddin, Muhammad Ikhtear, additional, Hossain, Motaher, additional, Khan, Ashraful I., additional, Chowdhury, Fahima, additional, Harris, Jason B., additional, Calderwood, Stephen B., additional, Ryan, Edward T., additional, Qadri, Firdausi, additional, and Leung, Daniel T., additional
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- 2022
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9. LPLUNC1 Modulates Innate Immune Responses to Vibrio cholerae
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Shin, Ok S., Uddin, Taher, Citorik, Robert, Wang, Jennifer P., Pelle, Patricia Della, Kradin, Richard L., Bingle, Colin D., Bingle, Lynne, Camilli, Andrew, Bhuiyan, Taufiqur R., Shirin, Tahmina, Ryan, Edward T., Calderwood, Stephen B., Finberg, Robert W., Qadri, Firdausi, LaRocque, Regina C., and Harris, Jason B.
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- 2011
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10. A Combination of Metagenomic and Cultivation Approaches Reveals Hypermutator Phenotypes within Vibrio cholerae-Infected Patients
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Levade, Inès, primary, Khan, Ashraful I., additional, Chowdhury, Fahima, additional, Calderwood, Stephen B., additional, Ryan, Edward T., additional, Harris, Jason B., additional, LaRocque, Regina C., additional, Bhuiyan, Taufiqur R., additional, Qadri, Firdausi, additional, Weil, Ana A., additional, and Shapiro, B. Jesse, additional
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- 2021
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11. Defining Polysaccharide-Specific Antibody Targets against Vibrio cholerae O139 in Humans following O139 Cholera and following Vaccination with a Commercial Bivalent Oral Cholera Vaccine, and Evaluation of Conjugate Vaccines Targeting O139
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Kamruzzaman, Mohammad, primary, Kelly, Meagan, additional, Charles, Richelle C., additional, Harris, Jason B., additional, Calderwood, Stephen B., additional, Akter, Aklima, additional, Biswas, Rajib, additional, Kaisar, M. Hasanul, additional, Bhuiyan, Taufiqur R., additional, Ivers, Louise C., additional, Ternier, Ralph, additional, Jerome, Jean-Gregory, additional, Pfister, Hélene B., additional, Lu, Xiaowei, additional, Soliman, Sameh E., additional, Ruttens, Bart, additional, Saksena, Rina, additional, Mečárová, Jana, additional, Čížová, Alžbeta, additional, Qadri, Firdausi, additional, Bystrický, Slavomír, additional, Kováč, Pavol, additional, Xu, Peng, additional, and Ryan, Edward T., additional
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- 2021
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12. Gut Microbiota and Development of Vibrio cholerae-Specific Long-Term Memory B Cells in Adults after Whole-Cell Killed Oral Cholera Vaccine
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Chac, Denise, primary, Bhuiyan, Taufiqur R., additional, Saha, Amit, additional, Alam, Mohammad M., additional, Salma, Umme, additional, Jahan, Nusrat, additional, Chowdhury, Fahima, additional, Khan, Ashraful I., additional, Ryan, Edward T., additional, LaRocque, Regina, additional, Harris, Jason B., additional, Qadri, Firdausi, additional, and Weil, Ana A., additional
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- 2021
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13. Parenteral Vaccination with a Cholera Conjugate Vaccine Boosts Vibriocidal and Anti-OSP Responses in Mice Previously Immunized with an Oral Cholera Vaccine
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Akter, Aklima, primary, Kelly, Meagan, additional, Charles, Richelle C., additional, Harris, Jason B., additional, Calderwood, Stephen B., additional, Bhuiyan, Taufiqur R., additional, Biswas, Rajib, additional, Xu, Peng, additional, Kováč, Pavol, additional, Qadri, Firdausi, additional, and Ryan, Edward T., additional
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- 2021
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14. HEV study protocol : design of a cluster-randomised, blinded trial to assess the safety, immunogenicity and effectiveness of the hepatitis E vaccine HEV 239 (Hecolin) in women of childbearing age in rural Bangladesh
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Zaman, K, Dudman, Susanne Gjeruldsen, Stene- Johansen, Kathrine, Qadri, Firdausi, Yunus, Md, Sandbu, Synne, Gurley, Emily S., Overbo, Joakim, Halle, Cathinka Lønning, Julin, Cathinka Halle, Dembinski, Jennifer Lynn, Nahar, Quamrun, Rahman, Anisur, Bhuiyan, Taufiqur R., Rahman, Mustafizur, Haque, Warda, Khan, Jahangir, Aziz, Asma, Khanam, Mahbuba, Streatfield, Peter Kim, and Clemens, John D.
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Adult ,Rural Population ,Viral Hepatitis Vaccines ,medicine.medical_specialty ,Hepatitis B vaccine ,Adolescent ,wa_395 ,wa_310 ,medicine.disease_cause ,qw_806 ,hepatobiliary disease ,immunology ,Young Adult ,Hepatitis E virus ,Pregnancy ,Epidemiology ,Medicine ,Humans ,Pregnancy Complications, Infectious ,Retrospective Studies ,Hepatitis ,Bangladesh ,Vaccines, Synthetic ,business.industry ,Public health ,Incidence ,Hepatobiliary disease ,Vaccination ,wc_536 ,General Medicine ,medicine.disease ,Prognosis ,Hepatitis E ,Infectious Diseases ,Family medicine ,Female ,epidemiology ,wa_309 ,business ,Viral hepatitis ,Follow-Up Studies - Abstract
IntroductionHepatitis E virus (HEV) is a leading cause of acute viral hepatitis in the developing world and is a public health problem, in particular among pregnant women, where it may lead to severe or fatal complications. A recombinant HEV vaccine, 239 (Hecolin; Xiamen Innovax Biotech, Xiamen, China), is licensed in China, but WHO calls for further studies to evaluate the safety and immunogenicity of this vaccine in vulnerable populations, and to evaluate protection in pregnancy. We are therefore conducting a phase IV trial to assess the effectiveness, safety and immunogenicity of the HEV 239 vaccine when given in women of childbearing age in rural Bangladesh, where HEV infection is endemic.Methods and analysisEnrolment of a target of approximately 20 000 non-pregnant women, aged 16–39 years, started on 2 October 2017 in Matlab, Bangladesh. Sixty-seven villages were randomised by village at a 1:1 ratio to receive either the HEV vaccine or the control vaccine (hepatitis B vaccine). A 3-dose vaccination series at 0, 1 and 6 months is ongoing, and women are followed up for 24 months. The primary outcome is confirmed HEV disease among pregnant women. After vaccination, participants are requested to report information about clinical hepatitis symptoms. Participants who become pregnant are visited at their homes every 2 weeks to collect information about pregnancy outcome and to screen for clinical hepatitis. All suspected hepatitis cases undergo laboratory testing for diagnostic evaluation. The incidence of confirmed HEV disease among pregnant and non-pregnant women will be compared between the HEV vaccinated and control groups, safety and immunogenicity of the vaccine will also be evaluated.Ethics and disseminationThe protocol was reviewed and approved by the International Centre for Diarrhoeal Disease Research, Bangladesh Research Review Committee and Ethical Review Committee, and the Directorate General of Drug Administration in Bangladesh, and by the Regional Ethics Committee in Norway. This article is based on the protocol version 2.2 dated 29 June 2017. We will present the results through peer-reviewed publications and at international conferences.Trial registration numberThe trial is registered at clinicaltrials.gov with the registry name “Effectiveness Trial to Evaluate Protection of Pregnant Women by Hepatitis E Vaccine in Bangladesh” and the identifierNCT02759991.
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- 2020
15. Evaluation of a Rapid Point-of-Care Multiplex Immunochromatographic Assay for the Diagnosis of Enteric Fever
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Kumar, Shailendra, primary, Nodoushani, Ariana, additional, Khanam, Farhana, additional, DeCruz, Alyssa T., additional, Lambotte, Paul, additional, Scott, Robert, additional, Bogoch, Isaac I., additional, Vaidya, Krista, additional, Calderwood, Stephen B., additional, Bhuiyan, Taufiqur R., additional, Esfandiari, Javan, additional, Ryan, Edward T., additional, Qadri, Firdausi, additional, Andrews, Jason R., additional, and Charles, Richelle C., additional
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- 2020
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16. Posttranslational Regulation of IL-23 Production Distinguishes the Innate Immune Responses to Live Toxigenic versus Heat-Inactivated Vibrio cholerae
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Weil, Ana A., primary, Ellis, Crystal N., additional, Debela, Meti D., additional, Bhuiyan, Taufiqur R., additional, Rashu, Rasheduzzaman, additional, Bourque, Daniel L., additional, Khan, Ashraful I., additional, Chowdhury, Fahima, additional, LaRocque, Regina C., additional, Charles, Richelle C., additional, Ryan, Edward T., additional, Calderwood, Stephen B., additional, Qadri, Firdausi, additional, and Harris, Jason B., additional
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- 2019
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17. Vibriocidal Titer and Protection From Cholera in Children
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Ritter, Alaina S, primary, Chowdhury, Fahima, additional, Franke, Molly F, additional, Becker, Rachel L, additional, Bhuiyan, Taufiqur R, additional, Khan, Ashraful I, additional, Saha, Nirod Chandra, additional, Ryan, Edward T, additional, Calderwood, Stephen B, additional, LaRocque, Regina C, additional, Harris, Jason B, additional, Qadri, Firdausi, additional, and Weil, Ana A, additional
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- 2019
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18. Su1930 Seroprevalence Trend of Helicobactor Pylori Infection Among Adult Population in a Highly Endemic Area of South Asia
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Rahman, Mohammed M., primary, Ahmed, Faruque, additional, Yusuf, Mohammad Abdullah, additional, Rowshon, AHM, additional, Ahmad, Mian Mashhud, additional, Kibria, Md. Golam, additional, Haque, Mazhar, additional, Qadri, Firdausi, additional, Bhuiyan, Taufiqur R., additional, and Hasan, Mahmud, additional
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- 2015
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19. Comparative Proteomic Analysis Reveals Activation of Mucosal Innate Immune Signaling Pathways during Cholera
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Ellis, Crystal N., primary, LaRocque, Regina C., additional, Uddin, Taher, additional, Krastins, Bryan, additional, Mayo-Smith, Leslie M., additional, Sarracino, David, additional, Karlsson, Elinor K., additional, Rahman, Atiqur, additional, Shirin, Tahmina, additional, Bhuiyan, Taufiqur R., additional, Chowdhury, Fahima, additional, Khan, Ashraful Islam, additional, Ryan, Edward T., additional, Calderwood, Stephen B., additional, Qadri, Firdausi, additional, and Harris, Jason B., additional
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- 2015
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20. Circulating Mucosal Associated Invariant T Cells Are Activated in Vibrio cholerae O1 Infection and Associated with Lipopolysaccharide Antibody Responses
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Leung, Daniel T., primary, Bhuiyan, Taufiqur R., additional, Nishat, Naoshin S., additional, Hoq, Mohammad Rubel, additional, Aktar, Amena, additional, Rahman, M. Arifur, additional, Uddin, Taher, additional, Khan, Ashraful I., additional, Chowdhury, Fahima, additional, Charles, Richelle C., additional, Harris, Jason B., additional, Calderwood, Stephen B., additional, Qadri, Firdausi, additional, and Ryan, Edward T., additional
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- 2014
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21. Th1 and Th17 Responses to Helicobacter pylori in Bangladeshi Infants, Children and Adults
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Bhuiyan, Taufiqur R., primary, Islam, M M. Towhidul, additional, Uddin, Taher, additional, Chowdhury, Mohiul I., additional, Janzon, Anders, additional, Adamsson, Jenni, additional, Lundin, Samuel B., additional, Qadri, Firdausi, additional, and Lundgren, Anna, additional
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- 2014
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22. Memory T-Cell Responses to Vibrio cholerae O1 Infection
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Weil, Ana A., primary, Arifuzzaman, Mohammad, additional, Bhuiyan, Taufiqur R., additional, LaRocque, Regina C., additional, Harris, Aaron M., additional, Kendall, Emily A., additional, Hossain, Azim, additional, Tarique, Abdullah A., additional, Sheikh, Alaullah, additional, Chowdhury, Fahima, additional, Khan, Ashraful I., additional, Murshed, Farhan, additional, Parker, Kenneth C., additional, Banerjee, Kalyan K., additional, Ryan, Edward T., additional, Harris, Jason B., additional, Qadri, Firdausi, additional, and Calderwood, Stephen B., additional
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- 2009
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23. Immunologic Responses to Vibrio cholerae in Patients Co-Infected with Intestinal Parasites in Bangladesh
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Harris, Jason B., primary, Podolsky, Michael J., additional, Bhuiyan, Taufiqur R., additional, Chowdhury, Fahima, additional, Khan, Ashraful I., additional, LaRocque, Regina C., additional, Logvinenko, Tanya, additional, Kendall, Jennifer, additional, Faruque, Abu S. G., additional, Nagler, Cathryn R., additional, Ryan, Edward T., additional, Qadri, Firdausi, additional, and Calderwood, Stephen B., additional
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- 2009
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24. Differential expression of intestinal membrane transporters in cholera patients
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Flach, Carl-Fredrik, Qadri, Firdausi, Bhuiyan, Taufiqur R., Alam, Nur H., Jennische, Eva, Holmgren, Jan, and Lönnroth, Ivar
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- 2007
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25. Broad Up-Regulation of Innate Defense Factors during Acute Cholera
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Flach, Carl-Fredrik, primary, Qadri, Firdausi, additional, Bhuiyan, Taufiqur R., additional, Alam, Nur H., additional, Jennische, Eva, additional, Lönnroth, Ivar, additional, and Holmgren, Jan, additional
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- 2007
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26. Th1 and Th17 Responses to Helicobacter pylori in Bangladeshi Infants, Children and Adults.
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Bhuiyan, Taufiqur R., Islam, M M. Towhidul, Uddin, Taher, Chowdhury, Mohiul I., Janzon, Anders, Adamsson, Jenni, Lundin, Samuel B., Qadri, Firdausi, and Lundgren, Anna
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HELICOBACTER pylori , *IMMUNE response , *T cells , *CYTOKINES , *MESSENGER RNA , *ENZYME-linked immunosorbent assay - Abstract
Both Th1 and Th17 cells are important components of the immune response to Helicobacter pylori (Hp) in adults, but less is known about T cell responses to Hp during early childhood, when the infection is often acquired. We investigated Th1 and Th17 type responses to Hp in adults, children and infants in Bangladesh, where Hp is highly endemic. IL-17 and IFN-γ mRNA levels in gastric biopsies from Hp-infected Bangladeshi adults were analyzed and compared to levels in infected and uninfected Swedish controls. Since biopsies could not be collected from infants and children, cytokine responses in Bangladeshi infants (6–12 months), children (3–5 years) and adults (>19 years) were instead compared by stimulating peripheral blood mononuclear cells (PBMCs) with a Hp membrane preparation (MP) and analyzing culture supernatants by ELISA and cytometric bead array. We found significantly higher expression of IL-17 and IFN-γ mRNA in gastric mucosa of Hp-infected Bangladeshi and Swedish adults compared to uninfected Swedish controls. PBMCs from all age groups produced IL-17 and IFN-γ after MP stimulation, but little Th2 cytokines. IL-17 and IFN-γ were primarily produced by CD4+ T cells, since CD4+ T cell depleted PBMCs produced reduced amounts of these cytokines. Infant cells produced significantly more IL-17, but similar levels of IFN-γ, compared to adult cells after MP stimulation. In contrast, polyclonal stimulation induced lower levels IL-17 and IFN-γ in infant compared to adult PBMCs and CD4+ T cells. The strong IL-17 production in infants after MP stimulation was paralleled by significantly higher production of the IL-17 promoting cytokine IL-1β from infant compared to adult PBMCs and monocytes. In conclusion, these results show that T cells can produce high levels of IL-17 and IFN-γ in response to Hp from an early age and indicate a potential role for IL-1β in promoting Th17 responses to Hp during infancy. [ABSTRACT FROM AUTHOR]
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- 2014
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27. Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells
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Kauffman, Robert C., Bhuiyan, Taufiqur R., Nakajima, Rie, Mayo-Smith, Leslie M., Rashu, Rasheduzzaman, Hoq, Mohammad Rubel, Chowdhury, Fahima, Khan, Ashraful Islam, Rahman, Atiqur, Bhaumik, Siddhartha K., Harris, Levelle, O'Neal, Justin T., Trost, Jessica F., Alam, Nur Haq, Jasinskas, Algis, Dotsey, Emmanuel, Kelly, Meagan, Charles, Richelle C., Xu, Peng, Kováč, Pavol, Calderwood, Stephen B., Ryan, Edward T., Felgner, Phillip L., Qadri, Firdausi, Wrammert, Jens, and Harris, Jason B.
- Abstract
We characterized the acute B cell response in adults with cholera by analyzing the repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. We found that the cholera-induced responses were characterized by high levels of somatic hypermutation and large clonal expansions. A majority of the expansions targeted cholera toxin (CT) or lipopolysaccharide (LPS). Using a novel proteomics approach, we were able to identify sialidase as another major antigen targeted by the antibody response to Vibrio cholerae infection. Antitoxin MAbs targeted both the A and B subunits, and most were also potent neutralizers of enterotoxigenic Escherichia coli heat-labile toxin. LPS-specific MAbs uniformly targeted the O-specific polysaccharide, with no detectable responses to either the core or the lipid moiety of LPS. Interestingly, the LPS-specific antibodies varied widely in serotype specificity and functional characteristics. One participant infected with the Ogawa serotype produced highly mutated LPS-specific antibodies that preferentially bound the previously circulating Inaba serotype. This demonstrates durable memory against a polysaccharide antigen presented at the mucosal surface and provides a mechanism for the long-term, partial heterotypic immunity seen following cholera.
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- 2016
- Full Text
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28. Memory T-Cell Responses to Vibrio choleraeO1 Infection
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Weil, Ana A., Arifuzzaman, Mohammad, Bhuiyan, Taufiqur R., LaRocque, Regina C., Harris, Aaron M., Kendall, Emily A., Hossain, Azim, Tarique, Abdullah A., Sheikh, Alaullah, Chowdhury, Fahima, Khan, Ashraful I., Murshed, Farhan, Parker, Kenneth C., Banerjee, Kalyan K., Ryan, Edward T., Harris, Jason B., Qadri, Firdausi, and Calderwood, Stephen B.
- Abstract
ABSTRACTVibrio choleraeO1 can cause diarrheal disease that may be life-threatening without treatment. Natural infection results in long-lasting protective immunity, but the role of T cells in this immune response has not been well characterized. In contrast, robust B-cell responses to V. choleraeinfection have been observed. In particular, memory B-cell responses to T-cell-dependent antigens persist for at least 1 year, whereas responses to lipopolysaccharide, a T-cell-independent antigen, wane more rapidly after infection. We hypothesize that protective immunity is mediated by anamnestic responses of memory B cells in the gut-associated lymphoid tissue, and T-cell responses may be required to generate and maintain durable memory B-cell responses. In this study, we examined B- and T-cell responses in patients with severe V. choleraeinfection. Using the flow cytometric assay of the specific cell-mediated immune response in activated whole blood, we measured antigen-specific T-cell responses using V. choleraeantigens, including the toxin-coregulated pilus (TcpA), a V. choleraemembrane preparation, and the V. choleraecytolysin/hemolysin (VCC) protein. Our results show that memory T-cell responses develop by day 7 after infection, a time prior to and concurrent with the development of B-cell responses. This suggests that T-cell responses to V. choleraeantigens may be important for the generation and stability of memory B-cell responses. The T-cell proliferative response to VCC was of a higher magnitude than responses observed to other V. choleraeantigens.
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- 2009
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29. Immunologic Responses to Vibrio cholerae in Patients Co-Infected with Intestinal Parasites in Bangladesh
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Bhuiyan, Taufiqur R., Chowdhury, Fahima, Khan, Ashraful I., Logvinenko, Tanya, Kendall, Jennifer, Faruque, Abu S. G., Nagler, Cathryn R., Qadri, Firdausi, Harris, Jason B., Podolsky, Michael Jeffrey, Larocque, Regina Celes, Ryan, Edward Thomas, and Calderwood, Stephen Beaven
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immunology ,immunity to infections ,immunomodulation ,infectious diseases ,neglected tropical diseases - Abstract
Background: Infection with intestinal helminths is common and may contribute to the decreased efficacy of Vibrio cholerae vaccines in endemic compared to non-endemic areas. However, the immunomodulatory effects of concomitant intestinal parasitic infection in cholera patients have not been systematically evaluated. Methods: We evaluated V. cholerae-specific immune responses in a cohort of patients with severe cholera. 361 patients completed 21 days of observation and 53 (15%) had evidence of a concomitant intestinal parasitic infection based on direct microscopy. Although there were no significant differences in the vibriocidal or lipopolysaccharide (LPS)-specific immune responses to V. cholerae, helminth-infected cholera patients had decreased fecal and serum IgA immune responses to the B subunit of cholera toxin (CTB) as well as a more modest decrease in serum IgG response to CTB. These findings remained significant for all classes of helminth infection and when controlling for potential confounding variables such as age and nutritional status. Although we hypothesized the differential effect on CTB and LPS immune responses was due to T-cell-dependent immunomodulatory effects of helminth infection, we did not find additional evidence to support a classic Th1 or Th2 polarization of the immune response to V. cholerae infection related to parasite infection. Conclusions/Significance: The finding that helminth infection has a profound association with the mucosal humoral immune response to V. cholerae has implications for the development of protective immunity in cholera-endemic areas and provides an additional basis for deworming programs in cholera-endemic areas. Additional studies, including further characterization of the role of T cells in the immune response to human V. cholerae infection and the development of an animal model of co-infection, may provide additional insight into the mechanisms underlying these findings.
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- 2009
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30. Single-Cell Analysis of the Plasmablast Response to Vibrio choleraeDemonstrates Expansion of Cross-Reactive Memory B Cells
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Kauffman, Robert C., Bhuiyan, Taufiqur R., Nakajima, Rie, Mayo-Smith, Leslie M., Rashu, Rasheduzzaman, Hoq, Mohammad Rubel, Chowdhury, Fahima, Khan, Ashraful Islam, Rahman, Atiqur, Bhaumik, Siddhartha K., Harris, Levelle, O'Neal, Justin T., Trost, Jessica F., Alam, Nur Haq, Jasinskas, Algis, Dotsey, Emmanuel, Kelly, Meagan, Charles, Richelle C., Xu, Peng, Kováč, Pavol, Calderwood, Stephen B., Ryan, Edward T., Felgner, Phillip L., Qadri, Firdausi, Wrammert, Jens, and Harris, Jason B.
- Abstract
ABSTRACTWe characterized the acute B cell response in adults with cholera by analyzing the repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. We found that the cholera-induced responses were characterized by high levels of somatic hypermutation and large clonal expansions. A majority of the expansions targeted cholera toxin (CT) or lipopolysaccharide (LPS). Using a novel proteomics approach, we were able to identify sialidase as another major antigen targeted by the antibody response to Vibrio choleraeinfection. Antitoxin MAbs targeted both the A and B subunits, and most were also potent neutralizers of enterotoxigenic Escherichia coliheat-labile toxin. LPS-specific MAbs uniformly targeted the O-specific polysaccharide, with no detectable responses to either the core or the lipid moiety of LPS. Interestingly, the LPS-specific antibodies varied widely in serotype specificity and functional characteristics. One participant infected with the Ogawa serotype produced highly mutated LPS-specific antibodies that preferentially bound the previously circulating Inaba serotype. This demonstrates durable memory against a polysaccharide antigen presented at the mucosal surface and provides a mechanism for the long-term, partial heterotypic immunity seen following cholera.IMPORTANCECholera is a diarrheal disease that results in significant mortality. While oral cholera vaccines are beneficial, they do not achieve equivalent protection compared to infection with Vibrio cholerae. Although antibodies likely mediate protection, the mechanisms of immunity following cholera are poorly understood, and a detailed understanding of antibody responses to cholera is of significance for human health. In this study, we characterized the human response to cholera at the single-plasmablast, monoclonal antibody level. Although this approach has not been widely applied to the study of human bacterial infection, we were able to uncover the basis of cross-reactivity between different V. choleraeserotypes and the likely impact of prior enterotoxigenic Escherichia coliexposure on the response to cholera, as well as identify novel antigenic targets. In addition to improving our understanding of the repertoire and function of the antibody response to cholera in humans, this study has implications for future cholera vaccination efforts.
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- 2016
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31. Vibrio cholerae O1 experiences mild bottlenecks through the gastrointestinal tract in some but not all cholera patients.
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Lypaczewski P, Chac D, Dunmire CN, Tandoc KM, Chowdhury F, Khan AI, Bhuiyan TR, Harris JB, LaRocque RC, Calderwood SB, Ryan ET, Qadri F, Shapiro BJ, and Weil AA
- Subjects
- Humans, Cholera Toxin genetics, Diarrhea microbiology, Phylogeny, Cholera microbiology, Vibrio cholerae O1 genetics, Vibrio cholerae O1 isolation & purification, Feces microbiology, Gastrointestinal Tract microbiology, Genome, Bacterial genetics, Genetic Variation
- Abstract
Vibrio cholerae O1 causes the diarrheal disease cholera, and the small intestine is the site of active infection. During cholera, cholera toxin is secreted from V. cholerae and induces a massive fluid influx into the small intestine, which causes vomiting and diarrhea. Typically, V. cholerae genomes are sequenced from bacteria passed in stool, but rarely from vomit, a fluid that may more closely represents the site of active infection. We hypothesized that V. cholerae O1 population bottlenecks along the gastrointestinal tract would result in reduced genetic variation in stool compared to vomit. To test this, we sequenced V. cholerae genomes from 10 cholera patients with paired vomit and stool samples. Genetic diversity was low in both vomit and stool, consistent with a single infecting population rather than coinfection with divergent V. cholerae O1 lineages. The amount of single-nucleotide variation decreased from vomit to stool in four patients, increased in two, and remained unchanged in four. The variation in gene presence/absence decreased between vomit and stool in eight patients and increased in two. Pangenome analysis of assembled short-read sequencing demonstrated that the toxin-coregulated pilus operon more frequently contained deletions in genomes from vomit compared to stool. However, these deletions were not detected by PCR or long-read sequencing, indicating that interpreting gene presence or absence patterns from short-read data alone may be incomplete. Overall, we found that V. cholerae O1 isolated from stool is genetically similar to V. cholerae recovered from the upper intestinal tract., Importance: Vibrio cholerae O1, the bacterium that causes cholera, is ingested in contaminated food or water and then colonizes the upper small intestine and is excreted in stool. Shed V. cholerae genomes from stool are usually studied, but V. cholerae isolated from vomit may be more representative of where V. cholerae colonizes in the upper intestinal epithelium. V. cholerae may experience bottlenecks, or large reductions in bacterial population sizes and genetic diversity, as it passes through the gut. Passage through the gut may select for distinct V. cholerae mutants that are adapted for survival and gut colonization. We did not find strong evidence for such adaptive mutations, and instead observed that passage through the gut results in modest reductions in V. cholerae genetic diversity, and only in some patients. These results fill a gap in our understanding of the V. cholerae life cycle, transmission, and evolution., Competing Interests: The authors declare no conflict of interest.
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- 2024
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32. Determinants of immune responses predictive of protection against shigellosis in an endemic zone: a systems analysis of antibody profiles and function.
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Bernshtein B, Kelly M, Cizmeci D, Zhiteneva JA, Macvicar R, Kamruzzaman M, Bhuiyan TR, Chowdhury F, Khan AI, Qadri F, Charles RC, Xu P, Kováč P, Clarkson KA, Kaminski RW, Alter G, and Ryan ET
- Abstract
Background: Shigella is the third leading global cause of moderate or severe diarrhoea among children younger than 5 years globally, and is the leading cause in children aged 24-59 months. The mechanism of protection against Shigella infection and disease in endemic areas is uncertain. We aimed to compare the Shigella-specific antibody responses in individuals living in Shigella-endemic and non-endemic areas, and to identify correlates of protection in a Shigella-endemic location., Methods: We applied a systems approach to retrospectively analyse serological responses to Shigella across endemic and non-endemic populations. We profiled serum samples collected from 44 individuals from the USA without previous exposure to Shigella and who were experimentally challenged with Shigella sonnei (non-endemic setting), and serum samples collected from 55 Peruvian army recruits (endemic setting). In the endemic setting, a subset of 37 samples collected from individuals infected with culture-confirmed Shigella flexneri 2a were divided into two groups: susceptible, which included individuals infected within 90 days of entering the camp (n=29); or resistant, which included individuals infected later than 90 days after entering the camp (n=8). We analysed Shigella-specific antibody isotype, subclass, and Fc receptor binding profiles across IpaB, IpaC, IpaD, and lipopolysaccharide from S flexneri 2a, 3a, and 6, and S sonnei, and O-specific polysaccharide (OSP) from S flexneri 2a and 3a and S sonnei. We also evaluated antibody-mediated complement deposition and innate immune cell activation. The main outcome of interest was the detection of antibody markers and functionality associated with protection against shigellosis in a high-burden endemic setting., Findings: Adults with endemic exposure to Shigella possessed broad and functional antibody responses across polysaccharide, glycolipid, and protein antigens compared with individuals from non-endemic regions. In a setting with high Shigella burden, elevated levels of OSP-specific Fcα receptor (FcαR) binding antibodies were associated with resistance to shigellosis, whereas total OSP-specific IgA was not, suggesting a potentially unique functionality. OSP-specific FcαR binding IgA found in resistant individuals activated bactericidal neutrophil functions including phagocytosis, degranulation, and production of reactive oxygen species. Moreover, IgA depletion from resistant serum significantly reduced binding of OSP-specific antibodies to FcαR and antibody-mediated activation of neutrophils and monocytes., Interpretation: Our findings suggest that OSP-specific functional IgA responses contribute to protective immunity against Shigella infection in a high-burden setting. These findings will assist in the development and evaluation of Shigella vaccines., Funding: US National Institutes of Health., Competing Interests: Declaration of interests GA is an employee of Moderna Therapeutics and holds equity in Leyden Labs and Systems Seromyx. All other authors declare no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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33. Vaccines for preventing enterotoxigenic Escherichia coli (ETEC) diarrhoea.
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Ahmed T, Bhuiyan TR, Zaman K, Sinclair D, and Qadri F
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- Adult, Child, Cholera Toxin adverse effects, Cholera Toxin immunology, Cholera Vaccines adverse effects, Developing Countries, Diarrhea microbiology, Humans, Randomized Controlled Trials as Topic, Travel, Vaccines, Inactivated adverse effects, Vaccines, Inactivated therapeutic use, Cholera Vaccines therapeutic use, Diarrhea prevention & control, Enterotoxigenic Escherichia coli immunology
- Abstract
Background: Infection with enterotoxigenic Escherichia coli (ETEC) bacteria is a common cause of diarrhoea in adults and children in developing countries and is a major cause of 'travellers' diarrhoea' in people visiting or returning from endemic regions. A killed whole cell vaccine (Dukoral®), primarily designed and licensed to prevent cholera, has been recommended by some groups to prevent travellers' diarrhoea in people visiting endemic regions. This vaccine contains a recombinant B subunit of the cholera toxin that is antigenically similar to the heat labile toxin of ETEC. This review aims to evaluate the clinical efficacy of this vaccine and other vaccines designed specifically to protect people against diarrhoea caused by ETEC infection., Objectives: To evaluate the efficacy, safety, and immunogenicity of vaccines for preventing ETEC diarrhoea., Search Methods: We searched the Cochrane Infectious Disease Group Specialized Register, the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, LILACS, and http://clinicaltrials.gov up to December 2012., Selection Criteria: Randomized controlled trials (RCTs) and quasi-RCTs comparing use of vaccines to prevent ETEC with use of no intervention, a control vaccine (either an inert vaccine or a vaccine normally given to prevent an unrelated infection), an alternative ETEC vaccine, or a different dose or schedule of the same ETEC vaccine in healthy adults and children living in endemic regions, intending to travel to endemic regions, or volunteering to receive an artificial challenge of ETEC bacteria., Data Collection and Analysis: Two authors independently assessed each trial for eligibility and risk of bias. Two independent reviewers extracted data from the included studies and analyzed the data using Review Manager (RevMan) software. We reported outcomes as risk ratios (RR) with 95% confidence intervals (CI). We assessed the quality of the evidence using the GRADE approach., Main Results: Twenty-four RCTs, including 53,247 participants, met the inclusion criteria. Four studies assessed the protective efficacy of oral cholera vaccines when used to prevent diarrhoea due to ETEC and seven studies assessed the protective efficacy of ETEC-specific vaccines. Of these 11 studies, seven studies presented efficacy data from field trials and four studies presented efficacy data from artificial challenge studies. An additional 13 trials contributed safety and immunological data only. Cholera vaccinesThe currently available, oral cholera killed whole cell vaccine (Dukoral®) was evaluated for protection of people against 'travellers' diarrhoea' in a single RCT in people arriving in Mexico from the USA. We did not identify any statistically significant effects on ETEC diarrhoea or all-cause diarrhoea (one trial, 502 participants, low quality evidence).Two earlier trials, one undertaken in an endemic population in Bangladesh and one undertaken in people travelling from Finland to Morocco, evaluated a precursor of this vaccine containing purified cholera toxin B subunit rather than the recombinant subunit in Dukoral®. Short term protective efficacy against ETEC diarrhoea was demonstrated, lasting for around three months (RR 0.43, 95% CI 0.26 to 0.71; two trials, 50,227 participants). This vaccine is no longer available. ETEC vaccinesAn ETEC-specific, killed whole cell vaccine, which also contains the recombinant cholera toxin B-subunit, was evaluated in people travelling from the USA to Mexico or Guatemala, and from Austria to Latin America, Africa, or Asia. We did not identify any statistically significant differences in ETEC-specific diarrhoea or all-cause diarrhoea (two trials, 799 participants), and the vaccine was associated with increased vomiting (RR 2.0, 95% CI 1.16 to 3.45; nine trials, 1528 participants). The other ETEC-specific vaccines in development have not yet demonstrated clinically important benefits., Authors' Conclusions: There is currently insufficient evidence from RCTs to support the use of the oral cholera vaccine Dukoral® for protecting travellers against ETEC diarrhoea. Further research is needed to develop safe and effective vaccines to provide both short and long-term protection against ETEC diarrhoea.
- Published
- 2013
- Full Text
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