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Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells
- Source :
- Kauffman, R. C., T. R. Bhuiyan, R. Nakajima, L. M. Mayo-Smith, R. Rashu, M. R. Hoq, F. Chowdhury, et al. 2016. “Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells.” mBio 7 (6): e02021-16. doi:10.1128/mBio.02021-16. http://dx.doi.org/10.1128/mBio.02021-16.
- Publication Year :
- 2016
- Publisher :
- American Society for Microbiology, 2016.
-
Abstract
- We characterized the acute B cell response in adults with cholera by analyzing the repertoire, specificity, and functional characteristics of 138 monoclonal antibodies (MAbs) generated from single-cell-sorted plasmablasts. We found that the cholera-induced responses were characterized by high levels of somatic hypermutation and large clonal expansions. A majority of the expansions targeted cholera toxin (CT) or lipopolysaccharide (LPS). Using a novel proteomics approach, we were able to identify sialidase as another major antigen targeted by the antibody response to Vibrio cholerae infection. Antitoxin MAbs targeted both the A and B subunits, and most were also potent neutralizers of enterotoxigenic Escherichia coli heat-labile toxin. LPS-specific MAbs uniformly targeted the O-specific polysaccharide, with no detectable responses to either the core or the lipid moiety of LPS. Interestingly, the LPS-specific antibodies varied widely in serotype specificity and functional characteristics. One participant infected with the Ogawa serotype produced highly mutated LPS-specific antibodies that preferentially bound the previously circulating Inaba serotype. This demonstrates durable memory against a polysaccharide antigen presented at the mucosal surface and provides a mechanism for the long-term, partial heterotypic immunity seen following cholera.
Details
- Language :
- English
- ISSN :
- 21507511
- Database :
- Digital Access to Scholarship at Harvard (DASH)
- Journal :
- Kauffman, R. C., T. R. Bhuiyan, R. Nakajima, L. M. Mayo-Smith, R. Rashu, M. R. Hoq, F. Chowdhury, et al. 2016. “Single-Cell Analysis of the Plasmablast Response to Vibrio cholerae Demonstrates Expansion of Cross-Reactive Memory B Cells.” mBio 7 (6): e02021-16. doi:10.1128/mBio.02021-16. http://dx.doi.org/10.1128/mBio.02021-16.
- Publication Type :
- Academic Journal
- Accession number :
- edshld.1.29739189
- Document Type :
- Journal Article
- Full Text :
- https://doi.org/10.1128/mBio.02021-16