1. One year exposure to Cd- and Pb-contaminated soil causes metal accumulation and alteration of global DNA methylation in rats
- Author
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Balazs Oroszlany, Shouta M.M. Nakayama, Kennedy Choongo, Hokuto Nakata, Takeshi Kuritani, Yared B. Yohannes, Kaampwe Muzandu, Nesta Bortey-Sam, John Yabe, Mitsuhiro Nakagawa, Mayumi Ishizuka, Yoshinori Ikenaka, and 27878368 - Ikenaka, Yoshinori
- Subjects
Male ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Toxicology ,01 natural sciences ,DNA methyltransferase ,Mining ,Cd ,DNA Methyltransferase 3A ,Metal ,Soil ,Pb contaminated soil ,medicine ,Animals ,Soil Pollutants ,DNA (Cytosine-5-)-Methyltransferases ,Respiratory system ,Rats, Wistar ,0105 earth and related environmental sciences ,Kidney ,DNA methylation ,Chemistry ,General Medicine ,Methylation ,DNA Methylation ,Pollution ,Rats ,medicine.anatomical_structure ,Lead ,Metal absorption ,visual_art ,Environmental chemistry ,Pb isotope ,Soil exposure ,visual_art.visual_art_medium ,Epigenetics ,Environmental Pollution ,Cadmium - Abstract
Metal pollution has been associated with anthropogenic activities, such as effluents and emissions from mines. Soil could be exposure route of wild rats to metals, especially in mining areas. The aim of this study was to verify whether soil exposure under environmentally relevant circumstances results in metal accumulation and epigenetic modifications. Wistar rats were divided to three groups: 1) control without soil exposure, 2) low-metal exposure group exposed to soil containing low metal levels (Pb: 75 mg/kg; Cd: 0.4), and 3) high-metal exposure group exposed to soil (Pb: 3750; Cd: 6). After 1 year of exposure, the metal levels, Pb isotopic values, and molecular indicators were measured. Rats in the high-group showed significantly greater concentrations of Pb and Cd in tissues. Higher accumulation factors (tissue/soil) of Cd than Pb were observed in the liver, kidney, brain, and lung, while the factor of Pb was higher in the tibia. The obtained results of metal accumulation ratios (lung/liver) and stable Pb isotope ratios in the tissues indicated that the respiratory exposure would account for an important share of metal absorption into the body. Genome-wide methylation status and DNA methyltransferase (Dnmt 3a/3b) mRNA expressions in testis were higher in the high-group, suggesting that exposure to soil caused metal accumulation and epigenetic alterations in rats. (C) 2019 Elsevier Ltd. All rights reserved.
- Published
- 2019