152 results on '"Lovering, Richard M."'
Search Results
2. CaMKII oxidation is a critical performance/disease trade-off acquired at the dawn of vertebrate evolution
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Wang, Qinchuan, Hernández-Ochoa, Erick O., Viswanathan, Meera C., Blum, Ian D., Do, Danh C., Granger, Jonathan M., Murphy, Kevin R., Wei, An-Chi, Aja, Susan, Liu, Naili, Antonescu, Corina M., Florea, Liliana D., Talbot, Jr., C. Conover, Mohr, David, Wagner, Kathryn R., Regot, Sergi, Lovering, Richard M., Gao, Peisong, Bianchet, Mario A., Wu, Mark N., Cammarato, Anthony, Schneider, Martin F., Bever, Gabriel S., and Anderson, Mark E.
- Published
- 2021
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3. Engineering functional and histological regeneration of vascularized skeletal muscle
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Gilbert-Honick, Jordana, Iyer, Shama R., Somers, Sarah M., Lovering, Richard M., Wagner, Kathryn, Mao, Hai-Quan, and Grayson, Warren L.
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- 2018
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4. Abnormalities in brain structure and biochemistry associated with mdx mice measured by in vivo MRI and high resolution localized 1H MRS
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Xu, Su, Shi, Da, Pratt, Stephen J.P., Zhu, Wenjun, Marshall, Andrew, and Lovering, Richard M.
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- 2015
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5. Myofiber Damage Precedes Macrophage Infiltration after in Vivo Injury in Dysferlin-Deficient A/J Mouse Skeletal Muscle
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Roche, Joseph A., Tulapurkar, Mohan E., Mueller, Amber L., van Rooijen, Nico, Hasday, Jeffrey D., Lovering, Richard M., and Bloch, Robert J.
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- 2015
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6. Recovery of altered neuromuscular junction morphology and muscle function in mdx mice after injury
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Pratt, Stephen J. P., Shah, Sameer B., Ward, Christopher W., Kerr, Jaclyn P., Stains, Joseph P., and Lovering, Richard M.
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- 2015
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7. An in vivo rodent model of contraction-induced injury in the quadriceps muscle
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Pratt, Stephen J.P., Lawlor, Michael W., Shah, Sameer B., and Lovering, Richard M.
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- 2012
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8. Fibroadipogenic progenitor cell response peaks prior to progressive fatty infiltration after rotator cuff tendon tear.
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Sahai, Amil, Jones, Derek L., Hughes, Marcus, Pu, Alex, Williams, Katrina, Iyer, Shama R., Rathinam, Chozha, Davis, Derik L., Lovering, Richard M., and Gilotra, Mohit N.
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ROTATOR cuff ,PROGENITOR cells ,TENDONS ,SUPRASPINATUS muscles ,COMPUTED tomography - Abstract
Fibroadipogenic progenitor (FAP) cells are implicated as a major source of fatty infiltration (FI) in murine rotator cuff (RC) injury, but FAP cell response after RC tear in a rabbit model is unknown. This study determined whether changes in FAP cell count after an RC tear predate muscle degeneration in a clinically relevant rabbit model. We hypothesized increases in FAP cell count correlate temporally with RC degeneration. New Zealand white rabbits (n = 26) were evaluated at 1, 2, 4, and 6 weeks after unilateral full‐thickness tenotomy of supraspinatus and infraspinatus tendons. FI area and adipocyte size were histologically analyzed, muscle density was measured by computerized tomography, and quantification of FAP cells was measured by flow cytometry and immunohistochemistry. The percentage of intrafascicular adipocyte area increased over time in supraspinatus muscle samples (p = 0.03), significantly between 1‐ and 6‐week samples (p = 0.04). There were no differences in perifascicular adipocyte area percentages between time points. Peak increase in FAP cell count occurred at 1‐week (p = 0.03), with a decrease in the following weeks. There was a negative correlation between supraspinatus adipocyte area and FAP cell count (p < 0.05). On computed tomography (CT) scan, maximal decrease in muscle density was observed in the 4th to 6th weeks. In summary, FAP cell response occurred early after tenotomy and did not correlate temporally with increases in FI. This suggests that FAP cell response may predate degenerative changes, and early targeting of FAPs before adipocyte maturation could blunt FI after RC tear. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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9. Tetanus toxin preserves skeletal muscle contractile force and size during limb immobilization
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Matthews, Christopher C., Lovering, Richard M., Bowen, Thomas G., and Fishman, Paul S.
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- 2014
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10. S100A1 promotes action potential-initiated calcium release flux and force production in skeletal muscle
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Prosser, Benjamin L., Hernandez-Ochoa, Erick O., Lovering, Richard M., Andronache, Zoita, Zimmer, Danna B., Melzer, Werner, and Schneider, Martin F.
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Calcium-binding proteins -- Physiological aspects ,Calcium-binding proteins -- Research ,Muscles -- Research ,Biological sciences - Abstract
The role of S100A1 in skeletal muscle is just beginning to be elucidated. We have previously shown that skeletal muscle fibers from S100A1 knockout (KO) mice exhibit decreased action potential (AP)-evoked [Ca.sup.2+] transients, and that S100A1 binds competitively with calmodulin to a canonical S100 binding sequence within the calmodulin-binding domain of the skeletal muscle ryanodine receptor. Using voltage clamped fibers, we found that [Ca.sup.2+] release was suppressed at all test membrane potentials in [S100A1.sup.-/-] fibers. Here we examine the role of S 100A1 during physiological AP-induced muscle activity, using an integrative approach spanning AP propagation to muscle force production. With the voltage-sensitive indicator di-8-aminonaphthylethenylpyridinium, we first demonstrate that the AP waveform is not altered in flexor digitorum brevis muscle fibers isolated from S100A1 KO mice. We then use a model for myoplasmic [Ca.sup.2+] binding and transport processes to calculate sarcoplasmic reticulum [Ca.sup.2+] release flux initiated by APs and demonstrate decreased release flux and greater inactivation of flux in KO fibers. Using in vivo stimulation of tibialis anterior muscles in anesthetized mice, we show that the maximal isometric force response to twitch and tetanic stimulation is decreased in [S100A1.sup.-/-] muscles. KO muscles also fatigue more rapidly upon repetitive stimulation than those of wild-type counterparts. We additionally show that fiber diameter, type, and expression of key excitation-contraction coupling proteins are unchanged in S100A1 KO muscle. We conclude that the absence of S100A1 suppresses physiological AP-induced [Ca.sup.2+] release flux, resulting in impaired contractile activation and force production in skeletal muscle. S100; excitation-contraction coupling; calcium signaling; muscle 10.1152/ajpcell.00180.2010.
- Published
- 2010
11. Extensive mononuclear infiltration and myogenesis characterize recovery of dysferlin-null skeletal muscle from contraction-induced injuries
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Roche, Joseph A., Lovering, Richard M., Roche, Renuka, Ru, Lisa W., Reed, Patrick W., and Bloch, Robert J.
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Dextran -- Health aspects ,Dextran -- Research ,Myosin -- Health aspects ,Muscle proteins -- Health aspects ,Myogenesis -- Analysis ,Biological sciences - Abstract
We studied the response of dysferlin-null and control skeletal muscle to large- and small-strain injuries to the ankle dorsiflexors in mice. We measured contractile torque and counted fibers retaining 10-kDa fluorescein dextran, necrotic fibers, macrophages, and fibers with central nuclei and expressing developmental myosin heavy chain to assess contractile function, membrane resealing, necrosis, inflammation, and myogenesis. We also studied recovery after blunting myogenesis with X-irradiation. We report that dysferlin-null myofibers retain 10-kDa dextran for 3 days after large-strain injury but are lost thereafter, following necrosis and inflammation. Recovery of dysferlin-null muscle requires myogenesis, which delays the return of contractile function compared with controls, which recover from large-strain injury by repairing damaged myofibers without significant inflammation, necrosis, or myogenesis. Recovery of control and dysferlin-null muscles from small-strain injury involved inflammation and necrosis followed by myogenesis, all of which were more pronounced in the dysferlin-null muscles, which recovered more slowly. Both control and dysferlin-null muscles also retained 10-kDa dextran for 3 days after small-strain injury. We conclude that dysferlin-null myofibers can survive contraction-induced injury for at least 3 days but are subsequently eliminated by necrosis and inflammation. Myogenesis to replace lost fibers does not appear to be significantly compromised in dysferlin-null mice. inflammation; limb-girdle muscular dystrophy type 2B; Miyoshi myopathy; muscle injury doi: 10.1152/ajpcell.00122.2009
- Published
- 2010
12. Ganglion Cyst in the Tarsal Tunnel
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FARBER, DANIEL C. and LOVERING, RICHARD M.
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- 2014
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13. Malformed mdx myofibers have normal cytoskeletal architecture yet altered EC coupling and stress-induced [Ca.sup.2+] signaling
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Lovering, Richard M., Michaelson, Luke, and Ward, Christopher W.
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Animal experimentation -- Usage ,Animal experimentation -- Methods ,Calcium channels -- Physiological aspects ,Calcium channels -- Research ,Muscular dystrophy -- Care and treatment ,Muscular dystrophy -- Research ,Muscles -- Physiological aspects ,Muscles -- Structure ,Muscles -- Research ,Biological sciences - Abstract
Skeletal muscle function is dependent on its highly regular structure. In studies of dystrophic (dy/dy) mice, the proportion of malformed myofibers decreases after prolonged whole muscle stimulation, suggesting that the malformed myofibers are more prone to injury. The aim of this study was to assess morphology and to measure excitation-contraction (EC) coupling ([Ca.sup.2+] transients) and susceptibility to osmotic stress ([Ca.sup.2+] sparks) of enzymatically isolated muscle fibers of the extensor digitorum longus (EDL) and flexor digitorum brevis (FDB) muscles from young (2-3 mo) and old (8-9 mo) mdx and age-matched control mice (C57BL10). In young mdx EDL, 6% of the myofibers had visible malformations (i.e., interfiber splitting, branched ends, midfiber appendages). In contrast, 65% of myofibers in old mdx EDL contained visible malformations. In the mdx FDB, malformation occurred in only 5% of young myofibers and 11% of old myofibers. Age-matched control mice did not display the altered morphology of mdx muscles. The membrane-associated and cytoplasmic cytoskeletal structures appeared normal in the malformed mdx myofibers. In mdx FDBs with significantly branched ends, an assessment of global, electrically evoked [Ca.sup.2+] signals (indo-1PE-AM) revealed an EC coupling deficit in myofibers with significant branching. Interestingly, peak amplitude of electrically evoked [Ca.sup.2+] release in the branch of the bifurcated mdx myofiber was significantly decreased compared with the trunk of the same myofiber. No alteration in the basal myoplasmic [Ca.sup.2+] concentration (i.e., indo ratio) was seen in malformed vs. normal mdx myofibers. Finally, osmotic stress induced the occurrence of [Ca.sup.2+] sparks to a greater extent in the malformed portions of myofibers, which is consistent with deficits in EC coupling control. In summary, our data show that aging mdx myofibers develop morphological malformations. These malformations are not associated with gross disruptions in cytoskeletal or t-tubule structure; however, alterations in myofiber [Ca.sup.2+] signaling are evident. calcium; muscular dystrophy; skeletal muscle
- Published
- 2009
14. Use of autologous platelet-rich plasma to treat muscle strain injuries
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Hammond, Jason W., Hinton, Richard Y., Curl, Leigh Ann, Muriel, Joaquin M., and Lovering, Richard M.
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Platelet-rich plasma therapy -- Usage ,Platelet-rich plasma therapy -- Patient outcomes ,Platelet-rich plasma therapy -- Research ,Sprains -- Care and treatment ,Sprains -- Research ,Health ,Sports and fitness - Published
- 2009
15. Architecture and fiber type of the pyramidalis muscle
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Lovering, Richard M. and Anderson, Larry D.
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- 2008
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16. Recovery of Function in Skeletal Muscle Following 2 Different Contraction-Induced Injuries
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Lovering, Richard M., Roche, Joseph A., Bloch, Robert J., and De Deyne, Patrick G.
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- 2007
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17. Effect of testosterone on the female anterior cruciate ligament
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Lovering, Richard M. and Romani, William A.
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Young women -- Health aspects ,Testosterone ,Morbidity ,Wounds and injuries ,Fluorescent antibody technique ,Immunofluorescence ,Anterior cruciate ligament ,Androgens ,Biological sciences - Abstract
Injuries to the anterior cruciate ligament (ACL) result in immediate and long-term morbidity and expense. Young women are more likely to sustain ACL injuries than men who participate in similar athletic and military activities. Although significant attention has focused on the role that female sex hormones may play in this disparity, it is still unclear whether the female ACL also responds to androgens. The purpose of this study was to determine whether the female ACL was an androgen-responsive tissue. To identify and localize androgen receptors in the female ACL, we used Western blotting and immunofluorescent labeling, respectively, of ACL tissue harvested during surgery from young women (n = 3). We then measured ACL stiffness and assessed total testosterone (T) and free [free androgen index (FAI)] testosterone concentrations, as well as relative estradiol to testosterone ratios ([E.sub.2]/T and [E.sub.2]/FAI) at three consecutive menstrual stages (n = 20). There were significant rank-order correlations between T (0.48, P = 0.031), FAI (0.44, P = 0.053), [E.sub.2]/T (-0.71, P < 0.001), [E.sub.2]/FAI (-0.63, P = 0.003), and ACL stiffness near ovulation. With the influences of the other variables controlled, there were significant negative partial rank-order correlations between ACL stiffness and [E.sub.2]/T (-0.72, P < 0.001) and [E.sub.2]/FAI (-0.59, P = 0.012). The partial order residuals for T and FAI were not significant. These findings suggest that the female ACL is an androgen-responsive tissue but that T and FAI are not independent predictors of ACL stiffness near ovulation. Instead, the relationship between T, FAI, and ACL stiffness was likely influenced by another hormone or sex hormone binding globulin. androgen receptor; gender differences; immunofluorescence; free androgen index
- Published
- 2005
18. Contractile function, sarcolemma integrity, and the loss of dystrophin after skeletal muscle eccentric contraction-induced injury
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Lovering, Richard M. and De Deyne, Patrick G.
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Cytoskeleton -- Research ,Biological sciences - Abstract
The purpose of this study was to evaluate the integrity of the muscle membrane and its associated cytoskeleton after a contraction-induced injury. A single eccentric contraction was performed in vivo on the tibialis anterior (TA) of male SpragueDawley rats at 900[degrees]/s throughout a 90[degrees]-arc of motion. Maximal tetanic tension ([P.sub.o]) of the TAs was assessed immediately and at 3, 7, and 21 days after the injury. To evaluate sarcolemmal integrity, we used an Evans blue dye (EBD) assay, and to assess structural changes, we used immunofluorescent labeling with antibodies against contractile (myosin, actin), cytoskeletal ([alpha]-actinin, desmin, dystrophin, [beta]-spectrin), integral membrane ([alpha]- and [beta]-dystroglycan, sarcoglycan), and extracellular (laminin, fibrunectin) proteins. Immediately after injury, Po was significantly reduced to 4.23 [+ or -] 0.22 N, compared with 8.24 [+ or -] 1.34 N in noninjured controls, and EBD was detected intracellularly in 54 [+ or -] 22% of fibers from the injured TA, compared with 0% in noninjured controls. We found a significant association between EBD-positive fibers and the loss of complete dystrophin labeling. The loss of dystrophin was notable because organization of other components of the subsarcolemmal cytoskeleton was affected minimally ([beta]-spectrin) or not at all ([alpha]- and [beta]-dystroglycan). Labeling with specific antibodies indicated that dystrophin's COOH terminus was selectively more affected than its rod domain. Twenty-one days after injury, contractile properties were normal, fibers did not contain EBD, and dystrophin organization and protein level returned to normal. These data indicate the selective vulnerability of dystrophin after a single eccentric contraction-induced injury and suggest a critical role of dystrophin in force transduction. muscle injury; dystrophin; cytoskeleton; sarcolemma
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- 2004
19. ARCHITECTURE OF HEALTHY AND DYSTROPHIC MUSCLES DETECTED BY OPTICAL COHERENCE TOMOGRAPHY
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LOVERING, RICHARD M., SHAH, SAMEER B., PRATT, STEPHEN J.P., GONG, WEI, and CHEN, YU
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- 2013
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20. Effects of in vivo injury on the neuromuscular junction in healthy and dystrophic muscles
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Pratt, Stephen J.P., Shah, Sameer B., Ward, Christopher W., Inacio, Mario P., Stains, Joseph P., and Lovering, Richard M.
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- 2013
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21. 1H MRS Measurements Of Myocellular Lipids After Injury To Healthy And Dystrophic Muscles: 1618: Board #151 June 1 2:00 PM - 3:30 PM
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Xu, Su, Shi, Da, and Lovering, Richard M.
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- 2011
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22. Keratin 18 Is Integral Part Of The Intermediate Filament Network In Skeletal Muscle: 1397: Board #133 June 1 9:30 AM - 11:00 AM
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Muriel, Joaquin M., Lovering, Richard M., O'Neill, Andrea, Kerr, Jaclyn P., and Bloch, Robert J.
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- 2011
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23. The contribution of contractile pre-activation to loss of function after a single lengthening contraction
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Lovering, Richard M., Hakim, Marc, Moorman, Claude T., III, and De Deyne, Patrick G.
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- 2005
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24. LOCATION OF MYOFIBER DAMAGE IN SKELETAL MUSCLE AFTER LENGTHENING CONTRACTIONS
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Lovering, Richard M., McMillan, Alan B., and Gullapalli, Rao P.
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- 2009
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25. The Nucleoskeleton: Crossroad of Mechanotransduction in Skeletal Muscle.
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Iyer, Shama R., Folker, Eric S., and Lovering, Richard M.
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NUCLEAR matrix ,MECHANOTRANSDUCTION (Cytology) ,SKELETAL muscle ,CYTOPLASMIC filaments ,NUCLEAR membranes ,CELLULAR signal transduction - Abstract
Intermediate filaments (IFs) are a primary structural component of the cytoskeleton extending throughout the muscle cell (myofiber). Mechanotransduction, the process by which mechanical force is translated into a biochemical signal to activate downstream cellular responses, is crucial to myofiber function. Mechanical forces also act on the nuclear cytoskeleton, which is integrated with the myofiber cytoskeleton by the linker of the nucleoskeleton and cytoskeleton (LINC) complexes. Thus, the nucleus serves as the endpoint for the transmission of force through the cell. The nuclear lamina, a dense meshwork of lamin IFs between the nuclear envelope and underlying chromatin, plays a crucial role in responding to mechanical input; myofibers constantly respond to mechanical perturbation via signaling pathways by activation of specific genes. The nucleus is the largest organelle in cells and a master regulator of cell homeostasis, thus an understanding of how it responds to its mechanical environment is of great interest. The importance of the cell nucleus is magnified in skeletal muscle cells due to their syncytial nature and the extreme mechanical environment that muscle contraction creates. In this review, we summarize the bidirectional link between the organization of the nucleoskeleton and the contractile features of skeletal muscle as they relate to muscle function. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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26. Fiber length variability within the flexor carpi ulnaris and flexor carpi radialis muscles: implications for surgical tendon transfer
- Author
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Fridén, Jan, Lovering, Richard M., and Lieber, Richard L.
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- 2004
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27. Muscle phenotype of a rat model of Duchenne muscular dystrophy.
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Iyer, Shama R., Xu, Su, Shah, Sameer B., and Lovering, Richard M.
- Abstract
Introduction: Our aim was to assess key muscle imaging and contractility parameters in the Duchenne muscular dystrophy (DMD) rat model (Dmd-KO rat), which have not yet been characterized sufficiently.Methods: We performed in-vivo magnetic resonance imaging (MRI) for thigh and leg muscles, and performed hematoxylin and eosin (H&E) staining and in-vivo muscle contractility testing in specific hindlimb muscles.Results: MRI prior to testing muscle contractility revealed multiple, unevenly distributed focal hyperintensities in the Dmd-KO rat quadriceps and tibialis anterior muscles. H&E staining showed corresponding areas of inflammation and ongoing regeneration. In-vivo contractile testing showed maximal force generated by Dmd-KO muscles was significantly lower, and susceptibility to injury was ~ two-fold greater in the Dmd-KO rats compared to wild-type (WT) rats.Discussion: Together, the MRI findings, histological findings, and the low strength and high susceptibility to injury in muscles support use of the Dmd-KO rat as an animal model of DMD. [ABSTRACT FROM AUTHOR]- Published
- 2020
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28. Abnormalities in Brain and Muscle Microstructure and Neurochemistry of the DMD Rat Measured by in vivo Diffusion Tensor Imaging and High Resolution Localized 1H MRS.
- Author
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Xu, Su, Tang, Shiyu, Li, Xin, Iyer, Shama R., and Lovering, Richard M.
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DIFFUSION tensor imaging ,HIGH resolution imaging ,BRAIN abnormalities ,NUCLEAR magnetic resonance spectroscopy ,CENTRAL nervous system ,TEMPORALIS muscle - Abstract
Duchenne muscular dystrophy (DMD) is an X-linked disorder caused by the lack of dystrophin with progressive degeneration of skeletal muscles. Most studies regarding DMD understandably focus on muscle, but dystrophin is also expressed in the central nervous system, potentially resulting in cognitive and behavioral changes. Animal models are being used for developing more comprehensive neuromonitoring protocols and clinical image acquisition procedures. The recently developed DMD rat is an animal model that parallels the progressive muscle wasting seen in DMD. Here, we studied the brain and temporalis muscle structure and neurochemistry of wild type (WT) and dystrophic (DMD) rats using magnetic resonance imaging and spectroscopy. Both structural and neurochemistry alterations were observed in the DMD rat brain and the temporalis muscle. There was a decrease in absolute brain volume (WT = 1579 mm
3 ; DMD = 1501 mm3 ; p = 0.039, Cohen's d = 1.867), but not normalized (WT = 4.27; DMD = 4.02; p = 0.306) brain volume. Diffusion tensor imaging (DTI) revealed structural alterations in the DMD temporalis muscle, with increased mean diffusivity (MD), axial diffusivity (AD), and radial diffusivity (RD). In the DMD rat thalamus, DTI revealed an increase in fractional anisotropy (FA) and a decrease in RD. Smaller normalized brain volume correlated to severity of muscle dystrophy (r = −0.975). Neurochemical changes in the DMD rat brain included increased GABA and NAA in the prefrontal cortex, and GABA in the hippocampus. Such findings could indicate disturbed motor and sensory signaling, resulting in a dysfunctional GABAergic neurotransmission, and an unstable osmoregulation in the dystrophin-null brain. [ABSTRACT FROM AUTHOR]- Published
- 2020
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29. Exosomes Isolated From Platelet-Rich Plasma and Mesenchymal Stem Cells Promote Recovery of Function After Muscle Injury.
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Iyer, Shama R., Scheiber, Amanda L., Yarowsky, Paul, Henn III, R. Frank, Otsuru, Satoru, and Lovering, Richard M.
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ANIMAL experimentation ,BIOCHEMISTRY ,BIOLOGICAL models ,BONE marrow ,COMPARATIVE studies ,CONVALESCENCE ,GENE expression ,HISTOLOGY ,INTRAMUSCULAR injections ,MUSCLE contraction ,MUSCLE proteins ,RATS ,SPRAINS ,STEM cells ,TORQUE ,TRANSFORMING growth factors-beta ,TREATMENT effectiveness ,EXOSOMES ,DESCRIPTIVE statistics ,TIBIALIS anterior ,IN vitro studies ,PLATELET-rich plasma ,IN vivo studies - Abstract
Background: Clinical use of platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs) has gained momentum as treatment for muscle injuries. Exosomes, or small cell–derived vesicles, could be helpful if they could deliver the same or better physiological effect without cell transplantation into the muscle. Hypothesis: Local delivery of exosomes derived from PRP (PRP-exos) or MSCs (MSC-exos) to injured muscles hastens recovery of contractile function. Study Design: Controlled laboratory study. Methods: In a rat model, platelets were isolated from blood, and MSCs were isolated from bone marrow and expanded in culture; exosomes from both were isolated through ultracentrifugation. The tibialis anterior muscles were injured in vivo using maximal lengthening contractions. Muscles were injected with PRP-exos or MSC-exos (immediately after injury and 5 and 10 days after injury); controls received an equal volume of saline. Histological and biochemical analysis was performed on tissues for all groups. Results: Injury resulted in a significant loss of maximal isometric torque (66% ± 3%) that gradually recovered over 2 weeks. Both PRP-exos and MSC-exos accelerated recovery, with similar faster recovery of contractile function over the saline-treated group at 5, 10, and 15 days after injury (P <.001). A significant increase in centrally nucleated fibers was seen with both types of exosome groups by day 15 (P <.01). Genes involved in skeletal muscle regeneration were modulated by different exosomes. Muscles treated with PRP-exos had increased expression of Myogenin gene (P <.05), whereas muscles treated with MSC-exos had reduced expression of TGF-β (P <.05) at 10 days after muscle injury. Conclusion: Exosomes derived from PRP or MSCs can facilitate recovery after a muscle strain injury in a small-animal model likely because of factors that can modulate inflammation, fibrosis, and myogenesis. Clinical Relevance: Given their small size, low immunogenicity, and ease with which they can be obtained, exosomes could represent a novel therapy for many orthopaedic ailments. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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30. Keratin 18 is an integral part of the intermediate filament network in murine skeletal muscle.
- Author
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Muriel, Joaquin M., O’Neill, Andrea, Kerr, Jaclyn P., Kleinhans-Welte, Emily, Lovering, Richard M., and Bloch, Robert J.
- Abstract
Intermediate filaments (IFs) contribute to force transmission, cellular integrity, and signaling in skeletal muscle. We previously identified keratin 19 (Krt19) as a muscle IF protein. We now report the presence of a second type I muscle keratin, Krt18. Krt18 mRNA levels are about half those for Krt19 and only 1:1,000th those for desmin; the protein was nevertheless detectable in immunoblots. Muscle function, measured by maximal isometric force in vivo, was moderately compromised in Krt18-knockout (Krt18-KO) or dominant-negative mutant mice (Krt18 DN), but structure was unaltered. Exogenous Krt18, introduced by electroporation, was localized in a reticulum around the contractile apparatus in wild-type muscle and to a lesser extent in muscle lacking Krt19 or desmin or both proteins. Exogenous Krt19, which was either reticular or aggregated in controls, became reticular more frequently in Krt19-null than in Krt18-null, desmin-null, or double-null muscles. Desmin was assembled into the reticulum normally in all genotypes. Notably, all three IF proteins appeared in overlapping reticular structures. We assessed the effect of Krt18 on susceptibility to injury in vivo by electroporating siRNA into tibialis anterior (TA) muscles of control and Krt19-KO mice and testing 2 wk later. Results showed a 33% strength deficit (reduction in maximal torque after injury) compared with siRNA-treated controls. Conversely, electroporation of siRNA to Krt19 into Krt18-null TA yielded a strength deficit of 18% after injury compared with controls. Our results suggest that Krt18 plays a complementary role to Krt19 in skeletal muscle in both assembling keratin-based filaments and transducing contractile force. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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31. Nerve lengthening and subsequent end‐to‐end repair yield more favourable outcomes compared with autograft repair of rat sciatic nerve defects.
- Author
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Howarth, Holly M., Kadoor, Adarsh, Salem, Rayeheh, Nicolds, Brogan, Adachi, Stephanie, Kanaris, Achilles, Lovering, Richard M., Brown, Justin M., and Shah, Sameer B.
- Published
- 2019
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32. Differential YAP nuclear signaling in healthy and dystrophic skeletal muscle.
- Author
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Iyer, Shama R., Shah, Sameer B., Ward, Christopher W., Stains, Joseph P., Spangenburg, Espen E., Folker, Eric S., and Lovering, Richard M.
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SKELETAL muscle ,NUCLEAR membranes ,DUCHENNE muscular dystrophy ,MUSCLE growth ,DYSTROPHIN ,EXTRACELLULAR matrix - Abstract
Mechanical forces regulate muscle development, hypertrophy, and homeostasis. Force-transmitting structures allow mechanotransduction at the sarcolemma, cytoskeleton, and nuclear envelope. There is growing evidence that Yes-associated protein (YAP) serves as a nuclear relay of mechanical signals and can induce a range of downstream signaling cascades. Dystrophin is a sarcolemma-associated protein, and its absence underlies the pathology in Duchenne muscular dystrophy. We tested the hypothesis that the absence of dystrophin in muscle would result in reduced YAP signaling in response to loading. Following in vivo contractile loading in muscles of healthy (wild-type; WT) mice and mice lacking dystrophin (mdx), we performed Western blots of whole and fractionated muscle homogenates to examine the ratio of phospho (cytoplasmic) YAP to total YAP and nuclear YAP, respectively. We show that in vivo contractile loading induced a robust increase in YAP expression and its nuclear localization in WT muscles. Surprisingly, in mdx muscles, active YAP expression was constitutively elevated and unresponsive to load. Results from qRT-PCR analysis support the hyperactivation of YAP in vivo in mdx muscles, as evidenced by increased gene expression of YAP downstream targets. In vitro assays of isolated myofibers plated on substrates with high stiffness showed YAP nuclear labeling for both genotypes, indicating functional YAP signaling in mdx muscles. We conclude that while YAP signaling can occur in the absence of dystrophin, dystrophic muscles have altered mechanotransduction, whereby constitutively active YAP results in a failure to respond to load, which could be attributed to the increased state of "pre-stress" with increased cytoskeletal and extracellular matrix stiffness. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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33. Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness.
- Author
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Tarpey, Michael D., Valencia, Ana P., Jackson, Kathryn C., Amorese, Adam J., Balestrieri, Nicholas P., Renegar, Randall H., Pratt, Stephen J. P., Ryan, Terence E., McClung, Joseph M., Lovering, Richard M., and Spangenburg, Espen E.
- Subjects
SKELETAL muscle ,BRCA genes ,MUSCLE weakness ,DNA ligases ,RESPIRATORY muscles ,DNA repair - Abstract
Key points: Breast cancer 1 early onset gene codes for the DNA repair enzyme, breast cancer type 1 susceptibility protein (BRCA1). The gene is prone to mutations that cause a loss of protein function.BRCA1/Brca1 has recently been found to regulate several cellular pathways beyond DNA repair and is expressed in skeletal muscle.Skeletal muscle specific knockout of Brca1 in mice caused a loss of muscle quality, identifiable by reductions in muscle force production and mitochondrial respiratory capacity.Loss of muscle quality was associated with a shift in muscle phenotype and an accumulation of mitochondrial DNA mutations.These results demonstrate that BRCA1 is necessary for skeletal muscle function and that increased mitochondrial DNA mutations may represent a potential underlying mechanism. Recent evidence suggests that the breast cancer 1 early onset gene (BRCA1) influences numerous peripheral tissues, including skeletal muscle. The present study aimed to determine whether induced‐loss of the breast cancer type 1 susceptibility protein (Brca1) alters skeletal muscle function. We induced genetic ablation of exon 11 in the Brca1 gene specifically in the skeletal muscle of adult mice to generate skeletal muscle‐specific Brca1 homozygote knockout (Brca1KOsmi) mice. Brca1KOsmi exhibited kyphosis and decreased maximal isometric force in limb muscles compared to age‐matched wild‐type mice. Brca1KOsmi skeletal muscle shifted toward an oxidative muscle fibre type and, in parallel, increased myofibre size and reduced capillary numbers. Unexpectedly, myofibre bundle mitochondrial respiration was reduced, whereas contraction‐induced lactate production was elevated in Brca1KOsmi muscle. Brca1KOsmi mice accumulated mitochondrial DNA mutations and exhibited an altered mitochondrial morphology characterized by distorted and enlarged mitochondria, and these were more susceptible to swelling. In summary, skeletal muscle‐specific loss of Brca1 leads to a myopathy and mitochondriopathy characterized by reductions in skeletal muscle quality and a consequent kyphosis. Given the substantial impact of BRCA1 mutations on cancer development risk in humans, a parallel loss of BRCA1 function in patient skeletal muscle cells would potentially result in implications for human health. Key points: Breast cancer 1 early onset gene codes for the DNA repair enzyme, breast cancer type 1 susceptibility protein (BRCA1). The gene is prone to mutations that cause a loss of protein function.BRCA1/Brca1 has recently been found to regulate several cellular pathways beyond DNA repair and is expressed in skeletal muscle.Skeletal muscle specific knockout of Brca1 in mice caused a loss of muscle quality, identifiable by reductions in muscle force production and mitochondrial respiratory capacity.Loss of muscle quality was associated with a shift in muscle phenotype and an accumulation of mitochondrial DNA mutations.These results demonstrate that BRCA1 is necessary for skeletal muscle function and that increased mitochondrial DNA mutations may represent a potential underlying mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
34. Fatty Infiltration Is a Prognostic Marker of Muscle Function After Rotator Cuff Tear.
- Author
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Valencia, Ana P., Lai, Jim K., Iyer, Shama R., Mistretta, Katherine L., Spangenburg, Espen E., Davis, Derik L., Lovering, Richard M., and Gilotra, Mohit N.
- Subjects
MUSCULAR atrophy ,ROTATOR cuff ,ANIMAL experimentation ,BIOMARKERS ,COLLAGEN ,FAT ,MUSCLE contraction ,PROGNOSIS ,RABBITS ,ROTATOR cuff injuries ,TENDON injuries ,TENOTOMY ,FIBROSIS ,ATROPHY ,DESCRIPTIVE statistics ,SUPRASPINATUS muscles ,KRUSKAL-Wallis Test ,ANATOMY ,DIAGNOSIS - Abstract
Background: Massive rotator cuff tears (RCTs) begin as primary tendon injuries and cause a myriad of changes in the muscle, including atrophy, fatty infiltration (FI), and fibrosis. However, it is unclear which changes are most closely associated with muscle function. Purpose: To determine if FI of the supraspinatus muscle after acute RCT relates to short-term changes in muscle function. Study Design: Controlled laboratory study. Methods: Unilateral RCTs were induced in female rabbits via tenotomy of the supraspinatus and infraspinatus. Maximal isometric force and rate of fatigue were measured in the supraspinatus in vivo at 6 and 12 weeks after tenotomy. Computed tomography scanning was performed, followed by histologic analysis of myofiber size, FI, and fibrosis. Results: Tenotomy resulted in supraspinatus weakness, reduced myofiber size, FI, and fibrosis, but no differences were evident between 6 and 12 weeks after tenotomy except for increased collagen content at 12 weeks. FI was a predictor of supraspinatus weakness and was strongly correlated to force, even after accounting for muscle cross-sectional area. While muscle atrophy accounted for the loss in force in tenotomized muscles with minimal FI, it did not account for the greater loss in force in tenotomized muscles with the most FI. Collagen content was not strongly correlated with maximal isometric force, even when normalized to muscle size. Conclusion: After RCT, muscle atrophy results in the loss of contractile force from the supraspinatus, but exacerbated weakness is observed with increased FI. Therefore, the level of FI can help predict contractile function of torn rotator cuff muscles. Clinical Relevance: Markers to predict contractile function of RCTs will help determine the appropriate treatment to improve functional recovery after RCTs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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- View/download PDF
35. Induced Cre-mediated knockdown of Brca1 in skeletal muscle reduces mitochondrial respiration and prevents glucose intolerance in adult mice on a high-fat diet.
- Author
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Jackson, Kathryn C., Tarpey, Michael D., Valencia, Ana P., Iñigo, Melissa R., Pratt, Stephen J., Patteson, Daniel J., McClung, Joseph M., Lovering, Richard M., Thomson, David M., and Spangenburg, Espen E.
- Published
- 2018
- Full Text
- View/download PDF
36. Non-invasive assessment of muscle injury in healthy and dystrophic animals with electrical impedance myography.
- Author
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Sanchez, Benjamin, Iyer, Shama R., Li, Jia, Kapur, Kush, Xu, Su, Rutkove, Seward B., and Lovering, Richard M.
- Abstract
Introduction: Dystrophic muscle is particularly susceptible to eccentric contraction-induced injury. We tested the hypothesis that electrical impedance myography (EIM) can detect injury induced by maximal-force lengthening contractions.Methods: We induced injury in the quadriceps of wild-type (WT) and dystrophic (mdx) mice with eccentric contractions using an established model.Results: mdx quadriceps had significantly greater losses in peak twitch and tetany compared with losses in WT quadriceps. Injured muscle showed a significant increase in EIM characteristic frequency in both WT (177 ± 7.7%) and mdx (167 ± 7.8%) quadriceps. EIM also revealed decreased extracellular resistance for both WT and mdx quadriceps after injury.Discussion: Our results show overall agreement between muscle function and EIM measurements of injured muscle, indicating that EIM is a viable tool to assess injury in dystrophic muscle. Muscle Nerve 56: E85-E94, 2017. [ABSTRACT FROM AUTHOR]- Published
- 2017
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37. Impaired contractile function of the supraspinatus in the acute period following a rotator cuff tear.
- Author
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Valencia, Ana P., Iyer, Shama R., Spangenburg, Espen E., Gilotra, Mohit N., and Lovering, Richard M.
- Subjects
ROTATOR cuff ,SHOULDER joint ,MYONEURAL junction ,NERVE endings ,SUPRASPINATUS muscles ,ADIPOSE tissues ,ANIMAL experimentation ,HUMAN body composition ,MUSCLE contraction ,MUSCULAR atrophy ,RATS ,STATISTICAL sampling ,FIBROSIS - Abstract
Background: Rotator cuff (RTC) tears are a common clinical problem resulting in adverse changes to the muscle, but there is limited information comparing histopathology to contractile function. This study assessed supraspinatus force and susceptibility to injury in the rat model of RTC tear, and compared these functional changes to histopathology of the muscle.Methods: Unilateral RTC tears were induced in male rats via tenotomy of the supraspinatus and infraspinatus. Maximal tetanic force and susceptibility to injury of the supraspinatus muscle were measured in vivo at day 2 and day 15 after tenotomy. Supraspinatus muscles were weighed and harvested for histologic analysis of the neuromuscular junction (NMJ), intramuscular lipid, and collagen.Results: Tenotomy resulted in eventual atrophy and weakness. Despite no loss in muscle mass at day 2 there was a 30% reduction in contractile force, and a decrease in NMJ continuity and size. Reduced force persisted at day 15, a time point when muscle atrophy was evident but NMJ morphology was restored. At day 15, torn muscles had decreased collagen-packing density and were also more susceptible to contraction-induced injury.Conclusion: Muscle size and histopathology are not direct indicators of overall RTC contractile health. Changes in NMJ morphology and collagen organization were associated with changes in contractile function and thus may play a role in response to injury. Although our findings are limited to the acute phase after a RTC tear, the most salient finding is that RTC tenotomy results in increased susceptibility to injury of the supraspinatus. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
38. Superparamagnetic Iron Oxide Nanoparticles in Musculoskeletal Biology.
- Author
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Iyer, Shama R., Xu, Su, Stains, Joseph P., Bennett, Craig H., and Lovering, Richard M.
- Published
- 2017
- Full Text
- View/download PDF
39. mTOR regulates peripheral nerve response to tensile strain.
- Author
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Love, James M., Bober, Brian G., Orozco, Elisabeth, White, Amanda T., Bremner, Shannon N., Lovering, Richard M., Schenk, Simon, and Shah, Sameer B.
- Subjects
PERIPHERAL nervous system ,MTOR protein ,MYELIN basic protein ,PHOSPHORYLATION ,CYTOPLASMIC filaments - Abstract
While excessive tensile strain can be detrimental to nerve function, strain can be a positive regulator of neuronal outgrowth. We used an in vivo rat model of sciatic nerve strain to investigate signaling mechanisms underlying peripheral nerve response to deformation. Nerves were deformed by 11% and did not demonstrate deficits in compound action potential latency or amplitude during or after 6 h of strain. As revealed by Western blotting, application of strain resulted in significant upregulation of mammalian target of rapamycin (mTOR) and S6 signaling in nerves, increased myelin basic protein (MBP) and β-actin levels, and increased phosphorylation of neurofilament subunit H (NF-H) compared with unstrained (sham) contralateral nerves (P β 0.05 for all comparisons, paired two-tailed t-test). Strain did not alter neuron-specific β3-tubulin or overall nerve tubulin levels compared with unstrained controls. Systemic rapamycin treatment, thought to selectively target mTOR complex 1 (mTORC1), suppressed mTOR/S6 signaling, reduced levels of MBP and overall tubulin, and decreased NF-H phosphorylation in nerves strained for 6 h, revealing a role for mTOR in increasing MBP expression and NF-H phosphorylation, and maintaining tubulin levels. Consistent with stretchinduced increases in MBP, immunolabeling revealed increased S6 signaling in Schwann cells of stretched nerves compared with unstretched nerves. In addition, application of strain to cultured adult dorsal root ganglion neurons showed an increase in axonal protein synthesis based on a puromycin incorporation assay, suggesting that neuronal translational pathways also respond to strain. This work has important implications for understanding mechanisms underlying nerve response to strain during development and regeneration. NEW & NOTEWORTHY: Peripheral nerves experience tensile strain (stretch) during development and movement. Excessive strain impairs neuronal function, but moderate strains are accommodated by nerves and can promote neuronal growth; mechanisms underlying these phenomena are not well understood. We demonstrated that levels of several structural proteins increase following physiological levels of nerve strain and that expression of a subset of these proteins is regulated by mTOR. Our work has important implications for understanding nerve development and strain-based regenerative strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
40. Altered nuclear dynamics in MDX myofibers.
- Author
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Iyer, Shama R., Shah, Sameer B., Valencia, Ana P., Schneider, Martin F., Hernández-Ochoa, Erick O., Stains, Joseph P., Blemker, Silvia S., and Lovering, Richard M.
- Subjects
DUCHENNE muscular dystrophy ,CYTOSKELETON - Abstract
Duchenne muscular dystrophy (DMD) is a genetic disorder in which the absence of dystrophin leads to progressive muscle degeneration and weakness. Although the genetic basis is known, the pathophysiology of dystrophic skeletal muscle remains unclear. We examined nuclear movement in wild-type (WT) and muscular dystrophy mouse model for DMD (MDX) (dystrophin-null) mouse myofibers. We also examined expression of proteins in the linkers of nucleoskeleton and cytoskeleton (LINC) complex, as well as nuclear transcriptional activity via histone H3 acetylation and polyadenylate-binding nuclear protein-1. Because movement of nuclei is not only LINC dependent but also microtubule dependent, we analyzed microtubule density and organization in WT and MDX myofibers, including the application of a unique 3D tool to assess microtubule core structure. Nuclei in MDX myofibers were more mobile than in WT myofibers for both distance traveled and velocity. MDX muscle shows reduced expression and labeling intensity of nesprin-1, a LINC protein that attaches the nucleus to the microtubule and actin cytoskeleton. MDX nuclei also showed altered transcriptional activity. Previous studies established that microtubule structure at the cortex is disrupted in MDX myofibers; our analyses extend these findings by showing that microtubule structure in the core is also disrupted. In addition, we studied malformed MDX myofibers to better understand the role of altered myofiber morphology vs. microtubule architecture in the underlying susceptibility to injury seen in dystrophic muscles. We incorporated morphological and microtubule architectural concepts into a simplified finite element mathematical model of myofiber mechanics, which suggests a greater contribution of myofiber morphology than microtubule structure to muscle biomechanical performance. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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- View/download PDF
41. NAD+ repletion improves muscle function in muscular dystrophy and counters global PARylation.
- Author
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Dongryeol Ryu, Hongbo Zhang, Ropelle, Eduardo R., Sorrentino, Vincenzo, Mázala, Davi A. G., Mouchiroud, Laurent, Marshall, Philip L., Campbell, Matthew D., Ali, Amir Safi, Knowels, Gary M., Bellemin, Stéphanie, Iyer, Shama R., Xu Wang, Gariani, Karim, Sauve, Anthony A., Cantó, Carles, Conley, Kevin E., Walter, Ludivine, Lovering, Richard M., and Chin, Eva R.
- Subjects
DUCHENNE muscular dystrophy ,NAD (Coenzyme) ,NEUROMUSCULAR diseases ,LABORATORY rats ,STEM cells ,MESSENGER RNA - Abstract
The article discusses a study which found that nicotinamide adenine dinucleotide (NAD
+ ) repletion is effective in improving muscle function in neuromuscular diseases like Duchene's muscular dystrophy (DMD) and delay both age-related and mdx muscle stem cell senescence. In the mice study, the correlations between the abundance of messenger ribonucleic acid (mRNA) transcripts related to mitochondrial biogenesis, the dystrophin-sarcoglycan complex, and NAD+ were observed.- Published
- 2016
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42. Alternating bipolar field stimulation identifies muscle fibers with defective excitability but maintained local Ca2+ signals and contraction.
- Author
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Hernández-Ochoa, Erick O., Vanegas, Camilo, Iyer, Shama R., Lovering, Richard M., and Schneider, Martin F.
- Subjects
MUSCLE physiology ,MUSCLE contraction ,CALCIUM ions ,ENZYMATIC analysis ,ELECTRIC stimulation ,ELECTROPHYSIOLOGY - Abstract
Background: Most cultured enzymatically dissociated adult myofibers exhibit spatially uniform (UNI) contractile responses and Ca
2+ transients over the entire myofiber in response to electric field stimuli of either polarity applied via bipolar electrodes. However, some myofibers only exhibit contraction and Ca2+ transients at alternating (ALT) ends in response to alternating polarity field stimulation. Here, we present for the first time the methodology for identification of ALT myofibers in primary cultures and isolated muscles, as well as a study of their electrophysiological properties. Results : We used high-speed confocal microscopic Ca2+ imaging, electric field stimulation, microelectrode recordings, immunostaining, and confocal microscopy to characterize the properties of action potential-induced Ca2+ transients, contractility, resting membrane potential, and staining of T-tubule voltage-gated Na+ channel distribution applied to cultured adult myofibers. Here, we show for the first time, with high temporal and spatial resolution, that normal control myofibers with UNI responses can be converted to ALT response myofibers by TTX addition or by removal of Na+ from the bathing medium, with reappearance of the UNI response on return of Na+ . Our results suggest disrupted excitability as the cause of ALT behavior and indicate that the ALT response is due to local depolarization-induced Ca2+ release, whereas the UNI response is triggered by action potential propagation over the entire myofiber. Consistent with this interpretation, local depolarizing monopolar stimuli give uniform (propagated) responses in UNI myofibers, but only local responses at the electrode in ALT myofibers. The ALT responses in electrically inexcitable myofibers are consistent with expectations of current spread between bipolar stimulating electrodes, entering (hyperpolarizing) one end of a myofiber and leaving (depolarizing) the other end of the myofiber. ALT responses were also detected in some myofibers within intact isolated whole muscles from wild-type and MDX mice, demonstrating that ALT responses can be present before enzymatic dissociation. Conclusions: We suggest that checking for ALT myofiber responsiveness by looking at the end of a myofiber during alternating polarity stimuli provides a test for compromised excitability of myofibers, and could be used to identify inexcitable, damaged or diseased myofibers by ALT behavior in healthy and diseased muscle. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
43. A method to test contractility of the supraspinatus muscle in mouse, rat, and rabbit.
- Author
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Iyer, Shama R., Pratt, Stephen J. P., Gilotra, Mohit N., Lovering, Richard M., and Valencia, Ana P.
- Subjects
CONTRACTILITY (Biology) ,SUPRASPINATUS muscles ,ROTATOR cuff ,ANIMAL models in research ,MUSCLE histology - Abstract
The rotator cuff (RTC) muscles not only generate movement but also provide important shoulder joint stability. RTC tears, particularly in the supraspinatus muscle, are a common clinical problem. Despite some biological healing after RTC repair, persistent problems include poor functional outcomes with high retear rates after surgical repair. Animal models allow further exploration of the sequela of RTC injury such as fibrosis, inflammation, and fatty infiltration, but there are few options regarding contractility for mouse, rat, and rabbit. Histological findings can provide a "direct measure" of damage, but the most comprehensive measure of the overall health of the muscle is contractile force. However, information regarding normal supraspinatus size and contractile function is scarce. Animal models provide the means to compare muscle histology, imaging, and contractility within individual muscles in various models of injury and disease, but to date, most testing of animal contractile force has been limited primarily to hindlimb muscles. Here, we describe an in vivo method to assess contractility of the supraspinatus muscle and describe differences in methods and representative outcomes for mouse, rat, and rabbit. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
44. Critical Role of Intracellular RyR1 Calcium Release Channels in Skeletal Muscle Function and Disease.
- Author
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Hernández-Ochoa, Erick O., Pratt, Stephen J. P., Lovering, Richard M., and Schneider, Martin F.
- Subjects
INTRACELLULAR calcium ,RYANODINE receptors ,VOLTAGE-gated ion channels ,SKELETAL muscle physiology ,MUSCLE diseases - Abstract
The skeletal muscle Ca
2+ release channel, also known as ryanodine receptor type 1 (RyR1), is the largest ion channel protein known and is crucial for effective skeletal muscle contractile activation. RyR1 function is controlled by Cav 1.1, a voltage gated Ca2+ channel that works mainly as a voltage sensor for RyR1 activity during skeletal muscle contraction and is also fine-tuned by Ca2+ , several intracellular compounds (e.g., ATP), and modulatory proteins (e.g., calmodulin). Dominant and recessive mutations in RyR1, as well as acquired channel alterations, are the underlying cause of various skeletal muscle diseases. The aim of this mini review is to summarize several current aspects of RyR1 function, structure, regulation, and to describe the most common diseases caused by hereditary or acquired RyR1 malfunction. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
45. Pre- and postsynaptic changes in the neuromuscular junction in dystrophic mice.
- Author
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Pratt, Stephen J. P., Valencia, Ana P., Le, Gloribel K., Shah, Sameer B., Lovering, Richard M., Rudolf, Rüdiger, and Jin-Hong Shin
- Subjects
MYONEURAL junction ,DUCHENNE muscular dystrophy ,ANIMAL models in research ,POSTSYNAPTIC potential ,NEUROPLASTICITY ,CELL morphology - Abstract
Duchenne muscular dystrophy (DMD) is a devastating neuromuscular disease in which weakness, increased susceptibility to muscle injury, and inadequate repair appear to underlie the pathology. While most attention has focused within the muscle fiber, we recently demonstrated in mdx mice (murine model for DMD) significant morphologic alterations at the motor endplate of the neuromuscular junction (NMJ) and corresponding NMJ transmission failure after injury. Here we extend these initial observations at the motor endplate to gain insight into the pre- vs. postsynaptic morphology, as well as the subsynaptic nuclei in healthy (WT) vs. mdx mice. We quantified the discontinuity and branching of the terminal nerve in adult mice. We report mdx- and age-dependent changes for discontinuity and an increase in branching when compared to WT. To examine mdx- and age-dependent changes in the relative localization of pre- and postsynaptic structures, we calculated NMJ occupancy, defined as the ratio of the footprint occupied by presynaptic vesicles vs. that of the underlying motor endplate. The normally congruent coupling between presynaptic and postsynaptic morphology was altered in mdx mice, independent of age. Finally we found an almost two-fold increase in the number of nuclei and an increase in density (nuclei/area) underlying the NMJ. These outcomes suggest substantial remodeling of the NMJ during dystrophic progression. This remodeling reflects plasticity in both pre- and postsynaptic contributors to NMJ structure, and thus perhaps also NM transmission and muscle function. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
46. SERCA1 overexpression minimizes skeletal muscle damage in dystrophic mouse models.
- Author
-
Mázala, Davi A. G., Pratt, Stephen J. P., Dapeng Chen, Molkentin, Jeffery D., Lovering, Richard M., and Chin, Eva R.
- Subjects
DUCHENNE muscular dystrophy ,TREATMENT of Duchenne muscular dystrophy ,SKELETAL muscle injuries ,GENETIC overexpression ,INTRACELLULAR calcium ,GENE expression in mammals ,DISEASE progression ,GENETICS - Abstract
Duchenne muscular dystrophy (DMD) is characterized by progressive muscle wasting secondary to repeated muscle damage and inadequate repair. Elevations in intracellular free Ca
2+ have been implicated in disease progression, and sarcoplasmic/endoplasmic reticulum Ca2+ -ATPase 1 (SERCA1) overexpression has been shown to ameliorate the dystrophic phenotype in mdx mice. The purpose of this study was to assess the effects of SERCA1 overexpression in the more severe mdx/Utr-/- mouse model of DMD. Mice overexpressing SERCA1 were crossed with mdx/Utr+/- mice to generate mdx/Utr-/- /+SERCA1 mice and compared with wild-type (WT), WT/+SERCA1, mdx/+SERCA1, and genotype controls. Mice were assessed at ~12 wk of age for changes in Ca2+ handling, muscle mass, quadriceps torque, markers of muscle damage, and response to repeated eccentric contractions. SERCA1- overexpressing mice had a two- to threefold increase in maximal sarcoplasmic reticulum Ca2+ -ATPase activity compared with WT which was associated with normalization in body mass for both mdx/+SERCA1 and mdx/Utr-/- /+SERCA1. Torque deficit in the quadriceps after eccentric injury was 2.7-fold greater in mdx/Utr-/- vs. WT mice, but only 1.5-fold greater in mdx/Utr-/- /+SERCA1 vs. WT mice, an attenuation of 44%. Markers of muscle damage (% centrally nucleated fibers, necrotic area, and serum creatine kinase levels) were higher in both mdx and mdx/Utr-/- vs. WT, and all were attenuated by overexpression of SERCA1. These data indicate that SERCA1 overexpression ameliorates functional impairments and cellular markers of damage in a more severe mouse model of DMD. These findings support targeting intracellular Ca2+ control as a therapeutic approach for DMD. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
47. Disruption of action potential and calcium signaling properties in malformed myofibers from dystrophin-deficient mice.
- Author
-
Hernández‐Ochoa, Erick O., Pratt, Stephen J. P., Garcia‐Pelagio, Karla P., Schneider, Martin F., and Lovering, Richard M.
- Subjects
DYSTROPHIN ,MUSCLE proteins ,CYTOSKELETAL proteins ,MEMBRANE proteins ,LABORATORY mice - Abstract
Duchenne muscular dystrophy ( DMD), the most common and severe muscular dystrophy, is caused by the absence of dystrophin. Muscle weakness and fragility (i.e., increased susceptibility to damage) are presumably due to structural instability of the myofiber cytoskeleton, but recent studies suggest that the increased presence of malformed/branched myofibers in dystrophic muscle may also play a role. We have previously studied myofiber morphology in healthy wild-type ( WT) and dystrophic ( MDX) skeletal muscle. Here, we examined myofiber excitability using high-speed confocal microscopy and the voltage-sensitive indicator di-8-butyl-amino-naphthyl-ethylene-pyridinium-propyl-sulfonate (di-8- ANEPPS) to assess the action potential ( AP) properties. We also examined AP-induced Ca
2+ transients using high-speed confocal microscopy with rhod-2, and assessed sarcolemma fragility using elastimetry. AP recordings showed an increased width and time to peak in malformed MDX myofibers compared to normal myofibers from both WT and MDX, but no significant change in AP amplitude. Malformed MDX myofibers also exhibited reduced AP-induced Ca2+ transients, with a further Ca2+ transient reduction in the branches of malformed MDX myofibers. Mechanical studies indicated an increased sarcolemma deformability and instability in malformed MDX myofibers. The data suggest that malformed myofibers are functionally different from myofibers with normal morphology. The differences seen in AP properties and Ca2+ signals suggest changes in excitability and remodeling of the global Ca2+ signal, both of which could underlie reported weakness in dystrophic muscle. The biomechanical changes in the sarcolemma support the notion that malformed myofibers are more susceptible to damage. The high prevalence of malformed myofibers in dystrophic muscle may contribute to the progressive strength loss and fragility seen in dystrophic muscles. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
48. Myopathic changes in murine skeletal muscle lacking synemin.
- Author
-
García-Pelagio, Karla P., Muriel, Joaquin, O'Neill, Andrea, Desmond, Patrick F., Lovering, Richard M., Lund, Linda, Bond, Meredith, and Bloch, Robert J.
- Subjects
CYTOSKELETON ,CYTOPLASMIC filaments ,SARCOLEMMA ,COSTAMERES ,CELL membranes ,SYNEMIN ,SKELETAL muscle ,MUSCLE diseases - Abstract
Diseases of striated muscle linked to intermediate filament (IF) proteins are associated with defects in the organization of the contractile apparatus and its links to costameres, which connect the sarcomeres to the cell membrane. Here we study the role in skeletal muscle of synemin, a type IV IF protein, by examining mice null for synemin (synm-null). Synm-null mice have a mild skeletal muscle phenotype. Tibialis anterior (TA) muscles show a significant decrease in mean fiber diameter, a decrease in twitch and tetanic force, and an increase in susceptibility to injury caused by lengthening contractions. Organization of proteins associated with the contractile apparatus and costameres is not significantly altered in the synm-null. Elastimetry of the sarcolemma and associated contractile apparatus in extensor digitorum longus myofibers reveals a reduction in tension consistent with an increase in sarcolemmal deformability. Although fatigue after repeated isometric contractions is more marked in TA muscles of synm-null mice, the ability of the mice to run uphill on a treadmill is similar to controls. Our results suggest that synemin contributes to linkage between costameres and the contractile apparatus and that the absence of synemin results in decreased fiber size and increased sarcolemmal deformability and susceptibility to injury. Thus synemin plays a moderate but distinct role in fast twitch skeletal muscle. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
49. Site-Specific Targeting of Platelet-Rich Plasma via Superparamagnetic Nanoparticles.
- Author
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Talaie, Tara, Pratt, Stephen J. P., Vanegas, Camilo, Su Xu, Henn III, R. Frank, Yarowsky, Paul, and Lovering, Richard M.
- Published
- 2015
- Full Text
- View/download PDF
50. Inhibition of YAP signaling improves recovery in injured skeletal muscle.
- Author
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Iyer, Shama R., Shah, Sameer B., Ward, Christopher W., Stains, Joseph P., Folker, Eric S., and Lovering, Richard M.
- Published
- 2022
- Full Text
- View/download PDF
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