108 results on '"Coletti, Dario"'
Search Results
2. PGC-1α in the myofibers regulates the balance between myogenic and adipogenic progenitors affecting muscle regeneration
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Beltrà, Marc, Pin, Fabrizio, Costamagna, Domiziana, Duelen, Robin, Renzini, Alessandra, Ballarò, Riccardo, Garcia-Castillo, Lorena, Iannuzzi, Ambra, Moresi, Viviana, Coletti, Dario, Sampaolesi, Maurilio, Penna, Fabio, and Costelli, Paola
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- 2022
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3. Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration
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Grifone, Raphaëlle, Saquet, Audrey, Desgres, Manon, Sangiorgi, Claudia, Gargano, Caterina, Li, Zhenlin, Coletti, Dario, and Shi, De-Li
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- 2021
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4. Vitamin D, muscle recovery, sarcopenia, cachexia, and muscle atrophy
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Garcia, Matheus, Seelaender, Marilia, Sotiropoulos, Athanassia, Coletti, Dario, and Lancha, Antonio Herbert, Jr
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- 2019
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5. Ormoni neuroipofisari: regolatori non canonici della struttura, funzione e omeostasi del muscolo
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Moresi, Viviana, Coletti, Dario, and Adamo, Sergio
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- 2019
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6. Ormoni neuroipofisari: regolatori non canonici della struttura, funzione e omeostasi del muscolo
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Moresi, Viviana, Coletti, Dario, and Adamo, Sergio
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- 2018
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7. Sex Differences in Inflammation and Muscle Wasting in Aging and Disease.
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Della Peruta, Chiara, Lozanoska-Ochser, Biliana, Renzini, Alessandra, Moresi, Viviana, Sanchez Riera, Carles, Bouché, Marina, and Coletti, Dario
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MYOSITIS ,SKELETAL muscle physiology ,MUSCLE aging ,WASTING syndrome ,SARCOPENIA ,MUSCULAR atrophy ,MUSCLE mass - Abstract
Only in recent years, thanks to a precision medicine-based approach, have treatments tailored to the sex of each patient emerged in clinical trials. In this regard, both striated muscle tissues present significant differences between the two sexes, which may have important consequences for diagnosis and therapy in aging and chronic illness. In fact, preservation of muscle mass in disease conditions correlates with survival; however, sex should be considered when protocols for the maintenance of muscle mass are designed. One obvious difference is that men have more muscle than women. Moreover, the two sexes differ in inflammation parameters, particularly in response to infection and disease. Therefore, unsurprisingly, men and women respond differently to therapies. In this review, we present an up-to-date overview on what is known about sex differences in skeletal muscle physiology and disfunction, such as disuse atrophy, age-related sarcopenia, and cachexia. In addition, we summarize sex differences in inflammation which may underly the aforementioned conditions because pro-inflammatory cytokines deeply affect muscle homeostasis. The comparison of these three conditions and their sex-related bases is interesting because different forms of muscle atrophy share common mechanisms; for instance, those responsible for protein dismantling are similar although differing in terms of kinetics, severity, and regulatory mechanisms. In pre-clinical research, exploring sexual dimorphism in disease conditions could highlight new efficacious treatments or recommend implementation of an existing one. Any protective factors discovered in one sex could be exploited to achieve lower morbidity, reduce the severity of the disease, or avoid mortality in the opposite sex. Thus, the understanding of sex-dependent responses to different forms of muscle atrophy and inflammation is of pivotal importance to design innovative, tailored, and efficient interventions. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Histone Deacetylases: Molecular Mechanisms and Therapeutic Implications for Muscular Dystrophies.
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Sandonà, Martina, Cavioli, Giorgia, Renzini, Alessandra, Cedola, Alessia, Gigli, Giuseppe, Coletti, Dario, McKinsey, Timothy A., Moresi, Viviana, and Saccone, Valentina
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MUSCULAR dystrophy ,DEACETYLASES ,DUCHENNE muscular dystrophy ,CLINICAL trials ,THERAPEUTICS ,FACIOSCAPULOHUMERAL muscular dystrophy ,SKELETAL muscle - Abstract
Histone deacetylases (HDACs) are enzymes that regulate the deacetylation of numerous histone and non-histone proteins, thereby affecting a wide range of cellular processes. Deregulation of HDAC expression or activity is often associated with several pathologies, suggesting potential for targeting these enzymes for therapeutic purposes. For example, HDAC expression and activity are higher in dystrophic skeletal muscles. General pharmacological blockade of HDACs, by means of pan-HDAC inhibitors (HDACi), ameliorates both muscle histological abnormalities and function in preclinical studies. A phase II clinical trial of the pan-HDACi givinostat revealed partial histological improvement and functional recovery of Duchenne Muscular Dystrophy (DMD) muscles; results of an ongoing phase III clinical trial that is assessing the long-term safety and efficacy of givinostat in DMD patients are pending. Here we review the current knowledge about the HDAC functions in distinct cell types in skeletal muscle, identified by genetic and -omic approaches. We describe the signaling events that are affected by HDACs and contribute to muscular dystrophy pathogenesis by altering muscle regeneration and/or repair processes. Reviewing recent insights into HDAC cellular functions in dystrophic muscles provides new perspectives for the development of more effective therapeutic approaches based on drugs that target these critical enzymes. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Sex and HDAC4 Differently Affect the Pathophysiology of Amyotrophic Lateral Sclerosis in SOD1-G93A Mice.
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Renzini, Alessandra, Pigna, Eva, Rocchi, Marco, Cedola, Alessia, Gigli, Giuseppe, Moresi, Viviana, and Coletti, Dario
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AMYOTROPHIC lateral sclerosis ,PATHOLOGICAL physiology ,MOTOR neuron diseases ,MUSCULAR atrophy ,SKELETAL muscle ,HISTONE deacetylase ,MICE ,FEMALES ,SEX (Biology) - Abstract
Amyotrophic Lateral Sclerosis (ALS) is a devastating adult-onset neurodegenerative disease, with ineffective therapeutic options. ALS incidence and prevalence depend on the sex of the patient. Histone deacetylase 4 (HDAC4) expression in skeletal muscle directly correlates with the progression of ALS, pointing to the use of HDAC4 inhibitors for its treatment. Contrarily, we have found that deletion of HDAC4 in skeletal muscle worsened the pathological features of ALS, accelerating and exacerbating skeletal muscle loss and negatively affecting muscle innervations in male SOD1-G93A (SOD1) mice. In the present work, we compared SOD1 mice of both sexes with the aim to characterize ALS onset and progression as a function of sex differences. We found a global sex-dependent effects on disease onset and mouse lifespan. We further investigated the role of HDAC4 in SOD1 females with a genetic approach, and discovered morpho-functional effects on skeletal muscle, even in the early phase of the diseases. The deletion of HDAC4 decreased muscle function and exacerbated muscle atrophy in SOD1 females, and had an even more dramatic effect in males. Therefore, the two sexes must be considered separately when studying ALS. [ABSTRACT FROM AUTHOR]
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- 2023
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10. The pro-myogenic environment provided by whole organ scale acellular scaffolds from skeletal muscle
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Perniconi, Barbara, Costa, Alessandra, Aulino, Paola, Teodori, Laura, Adamo, Sergio, and Coletti, Dario
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- 2011
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11. Functional Nutrients to Ameliorate Neurogenic Muscle Atrophy.
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Moresi, Viviana, Renzini, Alessandra, Cavioli, Giorgia, Seelaender, Marilia, Coletti, Dario, Gigli, Giuseppe, and Cedola, Alessia
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MUSCULAR atrophy ,AMYOTROPHIC lateral sclerosis ,MOTOR neurons ,SKELETAL muscle ,MUSCLE mass ,LABORATORY animals ,INDIVIDUALIZED medicine ,THERAPEUTICS - Abstract
Neurogenic muscle atrophy is a debilitating condition that occurs from nerve trauma in association with diseases or during aging, leading to reduced interaction between motoneurons and skeletal fibers. Current therapeutic approaches aiming at preserving muscle mass in a scenario of decreased nervous input include physical activity and employment of drugs that slow down the progression of the condition yet provide no concrete resolution. Nutritional support appears as a precious tool, adding to the success of personalized medicine, and could thus play a relevant part in mitigating neurogenic muscle atrophy. We herein summarize the molecular pathways triggered by denervation of the skeletal muscle that could be affected by functional nutrients. In this narrative review, we examine and discuss studies pertaining to the use of functional ingredients to counteract neurogenic muscle atrophy, focusing on their preventive or curative means of action within the skeletal muscle. We reviewed experimental models of denervation in rodents and in amyotrophic lateral sclerosis, as well as that caused by aging, considering the knowledge generated with use of animal experimental models and, also, from human studies. [ABSTRACT FROM AUTHOR]
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- 2022
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12. NF-κBediated Pax7 dysregulation in the muscle microenvironment promotes cancer cachexia
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He, Wei A., Berardi, Emanuele, Cardillo, Veronica M., Acharyya, Swarnali, Aulino, Paola, Thomas-Ahner, Jennifer, Wang, Jingxin, Bloomston, Mark, Muscarella, Peter, Nau, Peter, Shah, Nilay, Butchbach, Matthew E.R., Ladner, Katherine, Adamo, Sergio, Rudnicki, Michael A., Keller, Charles, Coletti, Dario, Montanaro, Federica, and Guttridge, Denis C.
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Cachexia -- Complications and side effects -- Genetic aspects -- Research ,DNA binding proteins -- Physiological aspects -- Research ,Muscles -- Physiological aspects -- Genetic aspects -- Research ,Health care industry - Abstract
Cachexia is a debilitating condition characterized by extreme skeletal muscle wasting that contributes significantly to morbidity and mortality. Efforts to elucidate the underlying mechanisms of muscle loss havpredominantly focused on [...]
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- 2013
13. Physiactisome: A New Nanovesicle Drug Containing Heat Shock Protein 60 for Treating Muscle Wasting and Cachexia.
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Di Felice, Valentina, Barone, Rosario, Trovato, Eleonora, D'Amico, Daniela, Macaluso, Filippo, Campanella, Claudia, Marino Gammazza, Antonella, Muccilli, Vera, Cunsolo, Vincenzo, Cancemi, Patrizia, Multhoff, Gabriele, Coletti, Dario, Adamo, Sergio, Farina, Felicia, and Cappello, Francesco
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HEAT shock proteins ,CACHEXIA ,MUSCULAR atrophy ,LEAN body mass ,EXTRACELLULAR vesicles - Abstract
Currently, no commercially available drugs have the ability to reverse cachexia or counteract muscle wasting and the loss of lean mass. Here, we report the methodology used to develop Physiactisome—a conditioned medium released by heat shock protein 60 (Hsp60)—overexpressing C2C12 cell lines enriched with small and large extracellular vesicles. We also present evidence supporting its use in the treatment of cachexia. Briefly, we obtain a nanovesicle-based secretion by genetically modifying C2C12 cell lines with an Hsp60-overexpressing plasmid. The secretion is used to treat naïve C2C12 cell lines. Physiactisome activates the expression of PGC-1α isoform 1, which is directly involved in mitochondrial biogenesis and muscle atrophy suppression, in naïve C2C12 cell lines. Proteomic analyses show Hsp60 localisation inside isolated nanovesicles and the localisation of several apocrine and merocrine molecules, with potential benefits for severe forms of muscle atrophy. Considering that Physiactisome can be easily obtained following tissue biopsy and can be applied to autologous muscle stem cells, we propose a potential nanovesicle-based anti-cachexia drug that could mimic the beneficial effects of exercise. Thus, Physiactisome may improve patient survival and quality of life. Furthermore, the method used to add Hsp60 into nanovesicles can be used to deliver other drugs or active proteins to vesicles. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Cytoplasmic HDAC4 regulates the membrane repair mechanism in Duchenne muscular dystrophy.
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Renzini, Alessandra, Marroncelli, Nicoletta, Cavioli, Giorgia, Di Francescantonio, Silvia, Forcina, Laura, Lambridis, Alessandro, Di Giorgio, Eros, Valente, Sergio, Mai, Antonello, Brancolini, Claudio, Giampietri, Claudia, Magenta, Alessandra, De Santa, Francesca, Adamo, Sergio, Coletti, Dario, and Moresi, Viviana
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- 2022
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15. NF-κB–mediated Pax7 dysregulation in the muscle microenvironment promotes cancer cachexia
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He, Wei A., Berardi, Emanuele, Cardillo, Veronica M., Acharyya, Swarnali, Aulino, Paola, Thomas-Ahner, Jennifer, Wang, Jingxin, Bloomston, Mark, Muscarella, Peter, Nau, Peter, Shah, Nilay, Butchbach, Matthew E.R., Ladner, Katherine, Adamo, Sergio, Rudnicki, Michael A., Keller, Charles, Coletti, Dario, Montanaro, Federica, and Guttridge, Denis C.
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- 2013
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16. Muscle cachexia is regulated by a p53-PW1/Peg3-dependent pathway
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Schwarzkopf, Martina, Coletti, Dario, Sassoon, David, and Marazzi, Giovanna
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Cachexia -- Research ,Tumor necrosis factor -- Structure ,Tumor necrosis factor -- Research ,Cell death -- Research ,Muscle diseases -- Research ,Biological sciences - Abstract
The tumor necrosis factor-[alpha] (TNF[alpha])-mediated inhibition of myogenic differentiation is found to be dependent on p53 through a PW1-mediated pathway. The p53 plays a novel role in mediating muscle stem cell behavior and muscle atrophy, and has pointed towards new targets for the therapeutic treatment of muscle wasting (cachexia).
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- 2006
17. Increase in cytosolic [Ca.sup.2+] induced by elevation of extracellular [Ca.sup.2+] in skeletal myogenic cells
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Naro, Fabio, De Arcangelis, Vania, Coletti, Dario, Molinaro, Mario, Zani, Bianca, Vassanelli, Stefano, Reggiani, Carlo, Teti, Anna, and Adamo, Sergio
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Cell membranes -- Research ,Myogenesis -- Research ,Cell differentiation -- Research ,Cell research ,Biological sciences - Abstract
Cytoplasmic [Ca.sup.2+] concentration ([[Ca.sup.2+]].sub.i]) variation is a key event in myoblast differentiation, but the mechanism by which it occurs is still debated. Here we show that increases of extracellular [Ca.sup.2+] concentration ([[[Ca.sup.2+]].sub.o]) produced membrane hyperpolarization and a concentration-dependent increase of [[[Ca.sup.2+]].sub.i] due to [Ca.sup.2+] influx across the plasma membrane. Responses were not related to inositol phosphate turnover and [Ca.sup.2+]-sensing receptor. [[[Ca.sup.2+]].sub.o]-induced [[[Ca.sup.2+]].sub.i] increase was inhibited by [Ca.sup.2+] channel inhibitors and appeared to be modulated by several kinase activities. [[[Ca.sup.2+]].sub.i] increase was potentiated by depletion of intracellular [Ca.sup.2+] stores and depressed by inactivation of the [Na.sup.+]/[Ca.sup.2+] exchanger. The response to arginine vasopressin (AVP), which induces inositol 1,4,5-trisphosphate-dependent [[[Ca.sup.2+]].sub.i] increase in L6-C5 cells, was not modified by high [[[Ca.sup.2+]].sub.o]. On the contrary, AVP potentiated the [[[Ca.sup.2+]].sub.i] increase in the presence of elevated [[[Ca.sup.2+]].sub.o]. Other clones of the L6 line as well as the rhabdomyosarcoma RD cell line and the satellite cell-derived C2-C12 line expressed similar responses to high [[[Ca.sup.2+]].sub.o], and the amplitude of the responses was correlated with the myogenic potential of the cells. calcium; myogenesis; calcium channels; sodium-calcium exchanger
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- 2003
18. Histone Deacetylases as Modulators of the Crosstalk Between Skeletal Muscle and Other Organs.
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Renzini, Alessandra, D'Onghia, Marco, Coletti, Dario, and Moresi, Viviana
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SKELETAL muscle ,DEACETYLASES ,HISTONE deacetylase ,MUSCLE metabolism ,PROTEIN metabolism ,ADIPOKINES ,FATTY liver - Abstract
Skeletal muscle plays a major role in controlling body mass and metabolism: it is the most abundant tissue of the body and a major source of humoral factors; in addition, it is primarily responsible for glucose uptake and storage, as well as for protein metabolism. Muscle acts as a metabolic hub, in a crosstalk with other organs and tissues, such as the liver, the brain, and fat tissue. Cytokines, adipokines, and myokines are pivotal mediators of such crosstalk. Many of these circulating factors modulate histone deacetylase (HDAC) expression and/or activity. HDACs form a numerous family of enzymes, divided into four classes based on their homology to their orthologs in yeast. Eleven family members are considered classic HDACs, with a highly conserved deacetylase domain, and fall into Classes I, II, and IV, while class III members are named Sirtuins and are structurally and mechanistically distinct from the members of the other classes. HDACs are key regulators of skeletal muscle metabolism, both in physiological conditions and following metabolic stress, participating in the highly dynamic adaptative responses of the muscle to external stimuli. In turn, HDAC expression and activity are closely regulated by the metabolic demands of the skeletal muscle. For instance, NAD+ levels link Class III (Sirtuin) enzymatic activity to the energy status of the cell, and starvation or exercise affect Class II HDAC stability and intracellular localization. SUMOylation or phosphorylation of Class II HDACs are modulated by circulating factors, thus establishing a bidirectional link between HDAC activity and endocrine, paracrine, and autocrine factors. Indeed, besides being targets of adipo-myokines, HDACs affect the synthesis of myokines by skeletal muscle, altering the composition of the humoral milieu and ultimately contributing to the muscle functioning as an endocrine organ. In this review, we discuss recent findings on the interplay between HDACs and circulating factors, in relation to skeletal muscle metabolism and its adaptative response to energy demand. We believe that enhancing knowledge on the specific functions of HDACs may have clinical implications leading to the use of improved HDAC inhibitors for the treatment of metabolic syndromes or aging. [ABSTRACT FROM AUTHOR]
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- 2022
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19. A tribute to Professor Sergio Adamo, Full Professor of Histology and Embryology at Sapienza University, Rome.
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Scicchitano, Bianca M., Bouchè, Marina, Nervi, Clara, and Coletti, Dario
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HISTOLOGY ,EMBRYOLOGY ,STRIATED muscle ,MUSCLE regeneration ,COLLEGE teachers - Abstract
Sergio Adamo prematurely left us on January 7th 2022, just one year after his retirement, leaving his family, friends and colleagues deeply sad and grieving. Sergio was a full Professor of Histology and Embryology at the Sapienza University of Rome. Since the foundation of the Institute of Histology and Embryology more than 50 years ago, he dedicated himself to the institution, research, and teaching with integrity, generosity, and a great sense of teamwork. Sergio's main research interests have been the mechanisms of myogenesis, muscle homeostasis and regeneration under normal and pathological conditions. Most relevant results obtained by Sergio and his collaborators indicate novel functions for the neurohypophyseal hormones, vasopressin and oxytocin, upon striated muscle differentiation, trophism, and homeostasis. Here we like to give the proper tribute to a mentor, a colleague and a sincere friend. He left an indelible mark on the professional and personal lives of all of us and his absence provokes a profound sense of emptiness. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Exercise-mediated reinnervation of skeletal muscle in elderly people: An update.
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Coletti, Claudia, Acosta, Gilberto F., Keslacy, Stefan, and Coletti, Dario
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OLDER people ,SKELETAL muscle ,MOTOR unit ,VASTUS lateralis ,MUSCLE mass - Abstract
Sarcopenia is defined by the loss of muscle mass and function. In aging sarcopenia is due to mild chronic inflammation but also to fiber-intrinsic defects, such as mitochondrial dysfunction. Agerelated sarcopenia is associated with physical disability and lowered quality of life. In addition to skeletal muscle, the nervous tissue is also affected in elderly people. With aging, type 2 fast fibers preferentially undergo denervation and are reinnervated by slow-twitch motor neurons. They spread forming new neuro-muscular junctions with the denervated fibers: the result is an increased proportion of slow fibers that group together since they are associated in the same motor unit. Grouping and fiber type shifting are indeed major histological features of aging skeletal muscle. Exercise has been proposed as an intervention for age-related sarcopenia due to its numerous beneficial effects on muscle mechanical and biochemical features. In 2013, a precursor study in humans was published in the European Journal of Translation Myology (formerly known as Basic and Applied Myology), highlighting the occurrence of reinnervation in the musculature of aged, exercise-trained individuals as compared to the matching control. This paper, entitled «Reinnervation of Vastus lateralis is increased significantly in seniors (70-years old) with a lifelong history of high-level exercise», is now being reprinted for the second issue of the «Ejtm Seminal Paper Series». In this short review we discuss those results in the light of the most recent advances confirming the occurrence of exercise-mediated reinnervation, ultimately preserving muscle structure and function in elderly people who exercise. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Selection, establishment and characterization of cell lines derived from a chemically-induced rat mammary heterogeneous tumor, by flow cytometry, transmission electron microscopy, and immunohistochemistry
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Teodori, Laura, Tagliaferri, Fausto, Stipa, Francesco, Valente, Maria Giovanna, Coletti, Dario, Manganelli, Angelo, Guglielmi, Mario, D'angelo, Luciana Santoro, Schäfer, Hartmut, and Göhde, Wolfgang
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- 2000
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22. In vitro proliferation and in vivo malignancy of cell lines simultaneously derived from a chemically-induced heterogeneous rat mammary tumor
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Tagliaferri, Fausto, Teodori, Laura, Valente, Maria Giovanna, Stipa, Francesco, Cucina, Alessandra, Göhde, Wolfgang, Coletti, Dario, Alo', Piero, and Stipa, Sergio
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- 2000
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23. Restoration versus reconstruction: cellular mechanisms of skin, nerve and muscle regeneration compared
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Coletti Dario, Teodori Laura, Lin Zhenlin, Beranudin Jean Francois, and Adamo Sergio
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Damage ,Necrosis ,Regeneration ,Differentiation ,Epithelial tissue ,Nervous tissue ,Skeletal muscle tissue ,Skin scar ,Stem cells ,Extra cellular matrix ,Medicine - Abstract
In tissues characterized by a high turnover or following acute injury, regeneration replaces damaged cells and is involved in adaptation to external cues, leading to homeostasis of many tissues during adult life. An understanding of the mechanics underlying tissue regeneration is highly relevant to regenerative medicine-based interventions. In order to investigate the existence a leitmotif of tissue regeneration, we compared the cellular aspects of regeneration of skin, nerve and skeletal muscle, three organs characterized by different types of anatomical and functional organization. Epidermis is a stratified squamous epithelium that migrates from the edge of the wound on the underlying dermis to rebuild lost tissue. Peripheral neurons are elongated cells whose neurites are organized in bundles, within an endoneurium of connective tissue; they either die upon injury or undergo remodeling and axon regrowth. Skeletal muscle is characterized by elongated syncytial cells, i.e. muscle fibers, that can temporarily survive in broken pieces; satellite cells residing along the fibers form new fibers, which ultimately fuse with the old ones as well as with each other to restore the previous organization. Satellite cell asymmetrical division grants a reservoir of undifferentiated cells, while other stem cell populations of muscle and non-muscle origin participate in muscle renewal. Following damage, all the tissues analyzed here go through three phases: inflammation, regeneration and maturation. Another common feature is the occurrence of cellular de-differentiation and/or differentiation events, including gene transcription, which are typical of embryonic development. Nonetheless, various strategies are used by different tissues to replace their lost parts. The epidermis regenerates ex novo, whereas neurons restore their missing parts; muscle fibers use a mixed strategy, based on the regrowth of missing parts through reconstruction by means of newborn fibers. The choice of either strategy is influenced by the anatomical, physical and chemical features of the cells as well as by the extracellular matrix typical of a given tissue, which points to the existence of differential, evolutionary-based mechanisms for specific tissue regeneration. The shared, ordered sequence of steps that characterize the regeneration processes examined suggests it may be possible to model this extremely important phenomenon to reproduce multicellular organisms.
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- 2013
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24. Molecular, cellular and physiological characterization of the cancer cachexia-inducing C26 colon carcinoma in mouse
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Baldi Alfonso, Spugnini Enrico P, Padula Fabrizio, Ramina Carla, Perniconi Barbara, Rizzuto Emanuele, Cardillo Veronica M, Berardi Emanuele, Aulino Paola, Faiola Fabio, Adamo Sergio, and Coletti Dario
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The majority of cancer patients experience dramatic weight loss, due to cachexia and consisting of skeletal muscle and fat tissue wasting. Cachexia is a negative prognostic factor, interferes with therapy and worsens the patients' quality of life by affecting muscle function. Mice bearing ectopically-implanted C26 colon carcinoma are widely used as an experimental model of cancer cachexia. As part of the search for novel clinical and basic research applications for this experimental model, we characterized novel cellular and molecular features of C26-bearing mice. Methods A fragment of C26 tumor was subcutaneously grafted in isogenic BALB/c mice. The mass growth and proliferation rate of the tumor were analyzed. Histological and cytofluorometric analyses were used to assess cell death, ploidy and differentiation of the tumor cells. The main features of skeletal muscle atrophy, which were highlighted by immunohistochemical and electron microscopy analyses, correlated with biochemical alterations. Muscle force and resistance to fatigue were measured and analyzed as major functional deficits of the cachectic musculature. Results We found that the C26 tumor, ectopically implanted in mice, is an undifferentiated carcinoma, which should be referred to as such and not as adenocarcinoma, a common misconception. The C26 tumor displays aneuploidy and histological features typical of transformed cells, incorporates BrdU and induces severe weight loss in the host, which is largely caused by muscle wasting. The latter appears to be due to proteasome-mediated protein degradation, which disrupts the sarcomeric structure and muscle fiber-extracellular matrix interactions. A pivotal functional deficit of cachectic muscle consists in increased fatigability, while the reported loss of tetanic force is not statistically significant following normalization for decreased muscle fiber size. Conclusions We conclude, on the basis of the definition of cachexia, that ectopically-implanted C26 carcinoma represents a well standardized experimental model for research on cancer cachexia. We wish to point out that scientists using the C26 model to study cancer and those using the same model to study cachexia may be unaware of each other's works because they use different keywords; we present strategies to eliminate this gap and discuss the benefits of such an exchange of knowledge.
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- 2010
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25. Tumor Microenvironment Autophagic Processes and Cachexia: The Missing Link?
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Gonçalves, Renata de Castro, Freire, Paula Paccielli, Coletti, Dario, and Seelaender, Marilia
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TUMOR microenvironment ,CACHEXIA ,MUSCLE mass ,TUMOR antigens ,QUALITY of life ,SKELETAL muscle injuries - Abstract
Cachexia is a syndrome that affects the entire organism and presents a variable plethora of symptoms in patients, always associated with continuous and involuntary degradation of skeletal muscle mass and function loss. In cancer, this syndrome occurs in 50% of all patients, while prevalence increases to 80% as the disease worsens, reducing quality of life, treatment tolerance, therapeutic response, and survival. Both chronic systemic inflammation and immunosuppression, paradoxically, correspond to important features in cachexia patients. Systemic inflammation in cachexia is fueled by the interaction between tumor and peripheral tissues with significant involvement of infiltrating immune cells, both in the peripheral tissues and in the tumor itself. Autophagy, as a process of regulating cellular metabolism and homeostasis, can interfere with the metabolic profile in the tumor microenvironment. Under a scenario of balanced autophagy in the tumor microenvironment, the infiltrating immune cells control cytokine production and secretion. On the other hand, when autophagy is unbalanced or dysfunctional within the tumor microenvironment, there is an impairment in the regulation of immune cell's inflammatory phenotype. The inflammatory phenotype upregulates metabolic consumption and cytokine production, not only in the tumor microenvironment but also in other tissues and organs of the host. We propose that cachexia-related chronic inflammation can be, at least, partly associated with the failure of autophagic processes in tumor cells. Autophagy endangers tumor cell viability by producing immunogenic tumor antigens, thus eliciting the immune response necessary to counteract tumor progression, while preventing the establishment of inflammation, a hallmark of cachexia. Comprehensive understanding of this complex functional dichotomy may enhance cancer treatment response and prevent/mitigate cancer cachexia. This review summarizes the recent available literature regarding the role of autophagy within the tumor microenvironment and the consequences eliciting the development of cancer cachexia. [ABSTRACT FROM AUTHOR]
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- 2021
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26. Polychlorobiphenyls Inhibit Skeletal Muscle Differentiation in Culture
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Coletti, Dario, Palleschi, Simonetta, Silvestroni, Leopoldo, Cannavò, Andrea, Vivarelli, Elisabetta, Tomei, Francesco, Molinaro, Mario, and Adamo, Sergio
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- 2001
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27. Vimentin as a target for the treatment of COVID-19.
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Zhenlin Li, Paulin, Denise, Lacolley, Patrick, Coletti, Dario, and Agbulut, Onnik
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- 2020
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28. Targeting RAGE prevents muscle wasting and prolongs survival in cancer cachexia.
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Chiappalupi, Sara, Sorci, Guglielmo, Vukasinovic, Aleksandra, Salvadori, Laura, Sagheddu, Roberta, Coletti, Dario, Renga, Giorgia, Romani, Luigina, Donato, Rosario, and Riuzzi, Francesca
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RECEPTOR for advanced glycation end products (RAGE) ,ADVANCED glycation end-products ,MITOGEN-activated protein kinases ,TUMOR necrosis factors ,ANGER ,MUSCLE regeneration - Abstract
Background: Cachexia, a multifactorial syndrome affecting more than 50% of patients with advanced cancer and responsible for ~20% of cancer‐associated deaths, is still a poorly understood process without a standard cure available. Skeletal muscle atrophy caused by systemic inflammation is a major clinical feature of cachexia, leading to weight loss, dampening patients' quality of life, and reducing patients' response to anticancer therapy. RAGE (receptor for advanced glycation end‐products) is a multiligand receptor of the immunoglobulin superfamily and a mediator of muscle regeneration, inflammation, and cancer. Methods: By using murine models consisting in the injection of colon 26 murine adenocarcinoma (C26‐ADK) or Lewis lung carcinoma (LLC) cells in BALB/c and C57BL/6 or Ager−/− (RAGE‐null) mice, respectively, we investigated the involvement of RAGE signalling in the main features of cancer cachexia, including the inflammatory state. In vitro experiments were performed using myotubes derived from C2C12 myoblasts or primary myoblasts isolated from C57BL/6 wild type and Ager−/− mice treated with the RAGE ligand, S100B (S100 calcium‐binding protein B), TNF (tumor necrosis factor)α±IFN (interferon) γ, and tumour cell‐ or masses‐conditioned media to analyse hallmarks of muscle atrophy. Finally, muscles of wild type and Ager−/− mice were injected with TNFα/IFNγ or S100B in a tumour‐free environment. Results: We demonstrate that RAGE is determinant to activate signalling pathways leading to muscle protein degradation in the presence of proinflammatory cytokines and/or tumour‐derived cachexia‐inducing factors. We identify the RAGE ligand, S100B, as a novel factor able to induce muscle atrophy per se via a p38 MAPK (p38 mitogen‐activated protein kinase)/myogenin axis and STAT3 (signal transducer and activator of transcription 3)‐dependent MyoD (myoblast determination protein 1) degradation. Lastly, we found that in cancer conditions, an increase in serum levels of tumour‐derived S100B and HMGB1 (high mobility group box 1) occurs leading to chronic activation/overexpression of RAGE, which induces hallmarks of cancer cachexia (i.e. muscle wasting, systemic inflammation, and release of tumour‐derived pro‐cachectic factors). Absence of RAGE in mice translates into reduced serum levels of cachexia‐inducing factors, delayed loss of muscle mass and strength, reduced tumour progression, and increased survival. Conclusions: RAGE is a molecular determinant in inducing the hallmarks of cancer cachexia, and molecular targeting of RAGE might represent a therapeutic strategy to prevent or counteract the cachectic syndrome. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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29. Smooth muscle integrin av contributes to the regulation of cell stiffness.
- Author
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Raoul, Alexandre, Belozertseva, Ekaterina, Lei Tian, Xiao Liu, Tone, Caterina Maria, Blanc, Jocelyne, Coletti, Dario, Henrion, Daniel, Regnault, Véronique, Lacolley, Patrick, Lacaze, Emmanuelle, Challande, Pascal, and Zhenlin Li
- Subjects
SMOOTH muscle ,INTEGRINS ,CAROTID artery - Abstract
Background: Integrin av is a receptor for adhesion proteins expressed at high density in vascular smooth muscle cells (VSMC) whose phenotypic modulation plays a crucial role in arterial ageing. Objectives: To define the arterial phenotype in mice conditionally inactivated for the integrin av subunit in VSMC and the role of this integrin in angiotensin II (Ang II)-induced arterial and VSMC stiffness. Methods and Results: We used a VSMC specific knock-out αv mouse model induced in adult mice by injection of tamoxifen. Trangenic mice (αvSMKO) and control littermates (Ctrl) were infused with Ang II (1.5 mg/kg/day) for 4 weeks. The pressure effect of Ang II was similar in Ctrl and αvSMKO mice. The carotid distensibility/pressure and elastic modulus/wall stress curves were similar in control and αvSMKO mice, indicating comparable arterial stiffness. Ang II treatment resulted in increased carotid stiffness in both groups without changes in vascular reactivity and myogenic tone. Electronic microscopy revealed less vesicles containing fiber-like materials in the SMCs of Ang II-treated αvSMKO carotids Elastic modulus of cultured VSMCs determined using atomic force microscopy was higher after Ang II treatment in cells from both groups. At baseline and after treatment, elastic modulus was higher in cells from αvSMKO mice than in cells from Ctrl mice. Conclusion: Inactivation of αv-containing integrins on VSMCs increases cell stiffness. The general mechanism involves a cross-talk between extracellular matrix, αv integrins and cytoskeletal complex. The lack of distensibility changes suggests additional changes at the level of αv-mediated dynamics of focal adhesion. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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30. The Mechanical Stimulation of Myotubes Counteracts the Effects of Tumor-Derived Factors Through the Modulation of the Activin/Follistatin Ratio.
- Author
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Baccam, Alexandra, Benoni-Sviercovich, Alexandra, Rocchi, Marco, Moresi, Viviana, Seelaender, Marilia, Li, Zhenlin, Adamo, Sergio, Xue, Zhigang, and Coletti, Dario
- Subjects
CANCER patients ,CACHEXIA ,MUSCULAR atrophy ,FOLLISTATIN ,CHRONIC diseases ,PROGENITOR cells - Abstract
Activin negatively affects muscle fibers and progenitor cells in aging (sarcopenia) and in chronic diseases characterized by severe muscle wasting (cachexia). High circulating activin levels predict poor survival in cancer patients. However, the relative impact of activin in mediating muscle atrophy and hampered homeostasis is still unknown. To directly assess the involvement of activin, and its physiological inhibitor follistatin, in cancer-induced muscle atrophy, we cultured C2C12 myotubes in the absence or in the presence of a mechanical stretching stimulus and in the absence or presence of C26 tumor-derived factors (CM), so as to mimic the mechanical stimulation of exercise and cancer cachexia, respectively. We found that CM induces activin release by myotubes, further exacerbating the negative effects of tumor-derived factors. In addition, mechanical stimulation is sufficient to counteract the adverse tumor-induced effects on muscle cells, in association with an increased follistatin/activin ratio in the cell culture medium, indicating that myotubes actively release follistatin upon stretching. Recombinant follistatin counteracts tumor effects on myotubes exclusively by rescuing fusion index, suggesting that it is only partially responsible for the stretch-mediated rescue. Therefore, besides activin, other tumor-derived factors may play a significant role in mediating muscle atrophy. In addition to increasing follistatin secretion mechanical stimulation induces additional beneficial responses in myotubes. We propose that in animal models of cancer cachexia and in cancer patients purely mechanical stimuli play an important role in mediating the rescue of the muscle homeostasis reported upon exercise. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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31. Skeletal Muscle: A Significant Novel Neurohypophyseal Hormone-Secreting Organ.
- Author
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Adamo, Sergio, Pigna, Eva, Lugarà, Rosamaria, Moresi, Viviana, Coletti, Dario, and Bouché, Marina
- Subjects
SKELETAL muscle ,NEUROHYPOPHYSIS ,MYOGENESIS ,CARBOHYDRATE metabolism ,VASOPRESSIN ,OXYTOCIN - Published
- 2019
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32. Revisiting the peculiar regional distribution of muscle fiber types in rat Sternomastoid Muscle.
- Author
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Ravara, Barbara, Gobbo, Valerio, Incendi, Damiana, Porzionato, Andrea, Macchi, Veronica, De Caro, Raffaele, Coletti, Dario, Martinello, Tiziana, and Patruno, Marco
- Subjects
STERNOCLEIDOMASTOID muscle ,MORPHOMETRICS ,MYOFIBRILS - Abstract
The sternomastoid (SM) muscle in rodents is known to have a peculiar distribution of fiber types with a steep gradient from surface to deep region. We here further characterize this peculiar regional distribution by quantitative histochemical morphometrys. In Hematoxylin- Eosin (H-E) stained transverse cryosections harvested in the medial portion of the muscle we counted around 10.000 myofibers with a mean diameter of 51.3±12.6 (μm). Cryisections of the SM stained by SDH reaction clearly show two distinct regions, toward the deep surface of the muscle a 40% area that contains packed SDH-positive myofibers, while the remaining area of the SM toward the external surface presents a more checker-board appearance. On the other hand, in the deep region of SM type 1 (slow contracting) muscle fibers, caracterized by positive acidic ATPase pH 4.35 reaction, are only the 24.5% of the fibers in the deep area of SM muscles, being restricted to the deepest region. The 75.5% of the myofibers in the deep region are of the fast contracting types (either 48.4% 2A, SDH -positive fibers or 27.1% 2B, SDH-negative fibers, respectively). As expected the 2B muscle fibers, acidic ATPase pH 4.3- negative and SDH-negative, present the largest size, while Type 1 fibers, acidic ATPase pH 4.3-positive and SDH-positive, present the smallest size in rat SM muscle. Based on present and previous observations, comparison of change in absolute number and/or percentage of the fiber types in any experimental model of muscle atrophy/hypertrophy/plasticity/pathology /recovery in the rat SM, and possibly of all mammals, will ask for morphometry of the whole muscle cross-sections, muscle sampling by bioptic approches will provide only comparable data on the size of the different types of muscle fibers. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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33. The Need for a Consensus on the Locution "Central Nuclei" in Striated Muscle Myopathies.
- Author
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Mazzotti, Anna L. and Coletti, Dario
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MEDICAL terminology ,MUSCLE diseases ,CELL nuclei ,TERMS & phrases ,REDUNDANCY (Linguistics) ,MEDICAL research - Abstract
The article presents the two options and justifications of the author's proposals on the need to establish an agreement on the term used in myopathy research as well as in clinical guidelines, "central nuclei" as a preferrable choice than using the expression "central." These include the redundancy on the use of ''centrally located nuclei'' despite than both expressions are correct in English, the advantage of using the word "central nuclei" in dealing word-count limits and short expression.
- Published
- 2016
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34. How Diet Intervention via Modulation of DNA Damage Response through MicroRNAs May Have an Effect on Cancer Prevention and Aging, an in Silico Study.
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Carotenuto, Felicia, Albertini, Maria C., Coletti, Dario, Vilmercati, Alessandra, Campanella, Luigi, Darzynkiewicz, Zbigniew, and Teodori, Laura
- Subjects
DNA damage ,DNA repair ,APOPTOSIS ,MICRORNA ,BIOACTIVE compounds - Abstract
The DNA damage response (DDR) is a molecular mechanism that cells have evolved to sense DNA damage (DD) to promote DNA repair, or to lead to apoptosis, or cellular senescence if the damage is too extensive. Recent evidence indicates that microRNAs (miRs) play a critical role in the regulation of DDR. Dietary bioactive compounds through miRs may affect activity of numerous genes. Among the most studied bioactive compounds modulating expression of miRs are epi-gallocatechin-3-gallate, curcumin, resveratrol and n3-polyunsaturated fatty acids. To compare the impact of these dietary compounds on DD/DDR network modulation, we performed a literature search and an in silico analysis by the DIANA-mirPathv3 software. The in silico analysis allowed us to identify pathways shared by different miRs involved in DD/DDR vis-à-vis the specific compounds. The results demonstrate that certain miRs (e.g., -146, -21) play a central role in the interplay among DD/DDR and the bioactive compounds. Furthermore, some specific pathways, such as "fatty acids biosynthesis/metabolism", "extracellular matrix-receptor interaction" and "signaling regulating the pluripotency of stem cells", appear to be targeted by most miRs affected by the studied compounds. Since DD/DDR and these pathways are strongly related to aging and carcinogenesis, the present in silico results of our study suggest that monitoring the induction of specific miRs may provide the means to assess the antiaging and chemopreventive properties of particular dietary compounds. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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35. Serum Response Factor in muscle tissues: from development to ageing.
- Author
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Coletti, Dario, Daou, Nissrine, Hassani, Medhi, Zhenlin Li, and Parlakian, Ara
- Subjects
- *
SERUM response factor , *MUSCLES , *AGING - Abstract
Skeletal, cardiac and smooth muscle cells share various common characteristic features. During development the embryonic mesodermal layer contribute at different proportions to the formation of these tissues. At the functional level, contractility as well as its decline during ageing, are also common features. Cytoskeletal components of these tissues are characterized by various actin isoforms that govern through their status (polymerised versus monomeric) and their interaction with the myosins the contractile properties of these muscles. Finally, at the molecular level, a set of different transcription factors with the notable exception of Serum Response Factor SRF- which is commonly enriched in the 3 types of muscle- drive and maintain the differentiation of these cells (Myf5, MyoD, Myogenin for skeletal muscle; Nkx2.5, GATA4 for cardiomyocytes). In this review, we will focus on the transcription factor SRF and its role in the homeostasis of cardiac, smooth and skeletal muscle tissues as well as its behaviour during the age related remodelling process of these tissues with a specific emphasis on animal models and human data when available. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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36. Spontaneous Physical Activity Downregulates Pax7 in Cancer Cachexia.
- Author
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Coletti, Dario, Aulino, Paola, Pigna, Eva, Barteri, Fabio, Moresi, Viviana, Annibali, Daniela, Adamo, Sergio, and Berardi, Emanuele
- Subjects
- *
EXERCISE physiology , *DOWNREGULATION , *CACHEXIA , *COLON cancer , *NF-kappa B , *GENETIC overexpression , *LABORATORY mice - Abstract
Emerging evidence suggests that the muscle microenvironment plays a prominent role in cancer cachexia. We recently showed that NF-kB-induced Pax7 overexpression impairs the myogenic potential of muscle precursors in cachectic mice, suggesting that lowering Pax7 expression may be beneficial in cancer cachexia. We evaluated the muscle regenerative potential after acute injury in C26 colon carcinoma tumor-bearing mice and healthy controls. Our analyses confirmed that the delayed muscle regeneration observed in muscles form tumor-bearing mice was associated with a persistent local inflammation and Pax7 overexpression. Physical activity is known to exert positive effects on cachectic muscles. However, the mechanism by which a moderate voluntary exercise ameliorates muscle wasting is not fully elucidated. To verify if physical activity affects Pax7 expression, we hosted control and C26-bearing mice in wheel-equipped cages and we found that voluntary wheel running downregulated Pax7 expression in muscles from tumor-bearing mice. As expected, downregulation of Pax7 expression was associated with a rescue of muscle mass and fiber size. Our findings shed light on the molecular basis of the beneficial effect exerted by a moderate physical exercise on muscle stem cells in cancer cachexia. Furthermore, we propose voluntary exercise as a physiological tool to counteract the overexpression of Pax7 observed in cancer cachexia. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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- View/download PDF
37. Regulation of skeletal muscle development and homeostasis by gene imprinting, histone acetylation and microRNA.
- Author
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Moresi, Viviana, Marroncelli, Nicoletta, Coletti, Dario, and Adamo, Sergio
- Abstract
Epigenetics is defined as heritable information other than the DNA sequence itself. The concept implies that the regulation of gene expression is a highly complex process in which epigenetics plays a major role that ranges from fine-tuning to permanent gene activation/deactivation. Skeletal muscle is the main tissue involved in locomotion and energy metabolism in the body, accounting for at least 40% of the body mass. Body mass and function vary according to age but also quickly adapt to both physiological and pathological cues. Besides transcriptional mechanisms that control muscle differentiation, postnatal growth and remodeling, there are numerous epigenetic mechanisms of regulation that modulate muscle gene expression. In this review, we describe and discuss only some of the mechanisms underlying epigenetic regulation, such as DNA methylation, histone modifications and microRNAs, which we believe are crucial to skeletal muscle development and disease. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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- View/download PDF
38. Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy.
- Author
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Li, Zhenlin, Parlakian, Ara, Coletti, Dario, Alonso-Martin, Sonia, Hourdé, Christophe, Joanne, Pierre, Gao-Li, Jacqueline, Blanc, Jocelyne, Ferry, Arnaud, Paulin, Denise, Xue, Zhigang, and Agbulut, Onnik
- Subjects
SYNEMIN ,SKELETAL muscle physiology ,HYPERTROPHY ,MYOSTATIN ,SATELLITE cells ,CREB protein ,CELLULAR signal transduction - Abstract
Synemin, a type IV intermediate filament (IF) protein, forms a bridge between IFs and cellular membranes. As an A-kinase-anchoring protein, it also provides temporal and spatial targeting of protein kinase A (PKA). However, little is known about its functional roles in either process. To better understand its functions in muscle tissue, we generated synemin-deficient (Synm
-/- ) mice. Synm-/- mice displayed normal development and fertility but showed a mild degeneration and regeneration phenotype in myofibres and defects in sarcolemma membranes. Following mechanical overload, Synm-/- mice muscles showed a higher hypertrophic capacity with increased maximal force and fatigue resistance compared with control mice. At the molecular level, increased remodelling capacity was accompanied by decreased myostatin (also known as GDF8) and atrogin (also known as FBXO32) expression, and increased follistatin expression. Furthermore, the activity of muscle-mass control molecules (the PKA RIIα subunit, p70S6K and CREB1) was increased in mutant mice. Finally, analysis of muscle satellite cell behaviour suggested that the absence of synemin could affect the balance between self-renewal and differentiation of these cells. Taken together, our results show that synemin is necessary to maintain membrane integrity and regulates signalling molecules during muscle hypertrophy. [ABSTRACT FROM AUTHOR]- Published
- 2014
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- View/download PDF
39. Muscle acellular scaffold as a biomaterial: effects on C2C12 cell differentiation and interaction with the murine host environment.
- Author
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Perniconi, Barbara, Coletti, Dario, Aulino, Paola, Costa, Alessandra, Aprile, Paola, Santacroce, Luigi, Chiaravalloti, Ernesto, Coquelin, Laura, Chevallier, Nathalie, Teodori, Laura, Adamo, Sergio, Marrelli, Massimo, and Tatullo, Marco
- Subjects
EXTRACELLULAR matrix ,TISSUE scaffolds ,BIOMATERIALS ,CELL differentiation ,REGENERATIVE medicine - Abstract
The extracellular matrix (ECM) of decellularized organs possesses the characteristics of the ideal tissue-engineering scaffold (i.e., histocompatibility, porosity, degradability, non-toxicity). We previously observed that the muscle acellular scaffold (MAS) is a pro-myogenic environment in vivo. In order to determine whether MAS, which is basically muscle ECM, behaves as a myogenic environment, regardless of its location, we analyzed MAS interaction with both muscle and non-muscle cells and tissues, to assess the effects of MAS on cell differentiation. Bone morphogenetic protein treatment of C2C12 cells cultured within MAS induced osteogenic differentiation in vitro, thus suggesting that MAS does not irreversibly commit cells to myogenesis. In vivo MAS supported formation of nascent muscle fibers when replacing a muscle (orthotopic position). However, heterotopically grafted MAS did not give rise to muscle fibers when transplanted within the renal capsule. Also, no muscle formation was observed when MAS was transplanted under the xiphoid process, in spite of the abundant presence of cells migrating along the laminin-based MAS structure. Taken together, our results suggest that MAS itself is not sufficient to induce myogenic differentiation. It is likely that the pro-myogenic environment of MAS is not strictly related to the intrinsic properties of the muscle scaffold (e.g., specific muscle ECM proteins). Indeed, it is more likely that myogenic stem cells colonizing MAS recognize a muscle environment that ultimately allows terminal myogenic differentiation. In conclusion, MAS may represent a suitable environment for muscle and non-muscle 3D constructs characterized by a highly organized structure whose relative stability promotes integration with the surrounding tissues. Our work highlights the plasticity of MAS, suggesting that it may be possible to consider MAS for a wider range of tissue engineering applications than the mere replacement of volumetric muscle loss. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
40. Native extracellular matrix: a new scaffolding platform for repair of damaged muscle.
- Author
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Teodori, Laura, Costa, Alessandra, Marzio, Rosa, Perniconi, Barbara, Coletti, Dario, Adamo, Sergio, Gupta, Bhuvanesh, and Tarnok, Attila
- Subjects
EXTRACELLULAR matrix ,MUSCLE injuries ,TISSUE engineering ,STEM cell research ,SKELETAL muscle - Abstract
Effective clinical treatments for volumetric muscle loss resulting from traumatic injury or resection of a large amount of muscle mass are not available to date. Tissue engineering may represent an alternative treatment approach. Decellularization of tissues and whole organs is a recently introduced platform technology for creating scaffolding materials for tissue engineering and regenerative medicine. The muscle stem cell niche is composed of a three-dimensional architecture of fibrous proteins, proteoglycans, and glycosaminoglycans, synthesized by the resident cells that form an intricate extracellular matrix (ECM) network in equilibrium with the surrounding cells and growth factors. A consistent body of evidence indicates that ECM proteins regulate stem cell differentiation and renewal and are highly relevant to tissue engineering applications. The ECM also provides a supportive medium for blood or lymphatic vessels and for nerves. Thus, the ECM is the nature's ideal biological scaffold material. ECM-based bioscaffolds can be recellularized to create potentially functional constructs as a regenerative medicine strategy for organ replacement or tissue repopulation. This article reviews current strategies for the repair of damaged muscle using bioscaffolds obtained from animal ECM by decellularization of small intestinal submucosa (SIS), urinary bladder mucosa (UB), and skeletal muscle, and proposes some innovative approaches for the application of such strategies in the clinical setting. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
41. Inactivation of Serum Response Factor Contributes To Decrease Vascular Muscular Tone and Arterial Stiffness in Mice.
- Author
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Galmiche, Guillaume, Labat, Carlos, Mericskay, Mathias, Aissa, Karima Ait, Blanc, Jocelyne, Retailleau, Kevin, Bourhim, Mustapha, Coletti, Dario, Loufrani, Laurent, Gao-Li, Jacqueline, Feil, Robert, Challande, Pascal, Henrion, Daniel, Decaux, Jean-François, Regnault, Véronique, Lacolley, Patrick, and Li, Zhenlin
- Published
- 2013
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42. Substrains of Inbred Mice Differ in Their Physical Activity as a Behavior.
- Author
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Coletti, Dario, Berardi, Emanuele, Aulino, Paola, Rossi, Eleonora, Moresi, Viviana, Zhenlin Li, and Adamo, Sergio
- Subjects
MICE breeding ,PHYSICAL activity ,ANIMAL behavior ,MEDICAL rehabilitation ,IMMUNOLOGY ,OBESITY - Published
- 2013
- Full Text
- View/download PDF
43. Molecular, cellular and physiologicalcharacterization of the cancer cachexia-inducingC26 colon carcinoma in mouse.
- Author
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Aulino, Paola, Berardi, Emanuele, Cardillo, Veronica M., Rizzuto, Emanuele, Perniconi, Barbara, Ramina, Carla, Padula, Fabrizio, Spugnini, Enrico P., Baldi, Alfonso, Faiola, Fabio, Adamo, Sergio, and Coletti, Dario
- Subjects
CANCER patients ,WEIGHT loss ,CACHEXIA ,COLON cancer ,CELL death - Abstract
Background: The majority of cancer patients experience dramatic weight loss, due to cachexia and consisting of skeletal muscle and fat tissue wasting. Cachexia is a negative prognostic factor, interferes with therapy and worsens the patients' quality of life by affecting muscle function. Mice bearing ectopically-implanted C26 colon carcinoma are widely used as an experimental model of cancer cachexia. As part of the search for novel clinical and basic research applications for this experimental model, we characterized novel cellular and molecular features of C26-bearing mice. Methods: A fragment of C26 tumor was subcutaneously grafted in isogenic BALB/c mice. The mass growth and proliferation rate of the tumor were analyzed. Histological and cytofluorometric analyses were used to assess cell death, ploidy and differentiation of the tumor cells. The main features of skeletal muscle atrophy, which were highlighted by immunohistochemical and electron microscopy analyses, correlated with biochemical alterations. Muscle force and resistance to fatigue were measured and analyzed as major functional deficits of the cachectic musculature. Results: We found that the C26 tumor, ectopically implanted in mice, is an undifferentiated carcinoma, which should be referred to as such and not as adenocarcinoma, a common misconception. The C26 tumor displays aneuploidy and histological features typical of transformed cells, incorporates BrdU and induces severe weight loss in the host, which is largely caused by muscle wasting. The latter appears to be due to proteasome-mediated protein degradation, which disrupts the sarcomeric structure and muscle fiber-extracellular matrix interactions. A pivotal functional deficit of cachectic muscle consists in increased fatigability, while the reported loss of tetanic force is not statistically significant following normalization for decreased muscle fiber size. Conclusions: We conclude, on the basis of the definition of cachexia, that ectopically-implanted C26 carcinoma represents a well standardized experimental model for research on cancer cachexia. We wish to point out that scientists using the C26 model to study cancer and those using the same model to study cachexia may be unaware of each other's works because they use different keywords; we present strategies to eliminate this gap and discuss the benefits of such an exchange of knowledge. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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44. Modulation of Caspase Activity Regulates Skeletal Muscle Regeneration and Function in Response to Vasopressin and Tumor Necrosis Factor.
- Author
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Moresi, Viviana, Garcia-Alvarez, Gisela, Pristerà, Alessandro, Rizzuto, Emanuele, Albertini, Maria C., Rocchi, Marco, Marazzi, Giovanna, Sassoon, David, Adamo, Sergio, and Coletti, Dario
- Subjects
HOMEOSTASIS ,MYOGENESIS ,TUMOR necrosis factors ,VASOPRESSIN ,MUSCLE regeneration ,MUSCLE diseases ,MUSCLE cells ,MYOBLASTS ,CELL culture - Abstract
Muscle homeostasis involves de novo myogenesis, as observed in conditions of acute or chronic muscle damage. Tumor Necrosis Factor (TNF) triggers skeletal muscle wasting in several pathological conditions and inhibits muscle regeneration. We show that intramuscular treatment with the myogenic factor Arg8-vasopressin (AVP) enhanced skeletal muscle regeneration and rescued the inhibitory effects of TNF on muscle regeneration. The functional analysis of regenerating muscle performance following TNF or AVP treatments revealed that these factors exerted opposite effects on muscle function. Principal component analysis showed that TNF and AVP mainly affect muscle tetanic force and fatigue. Importantly, AVP counteracted the effects of TNF on muscle function when delivered in combination with the latter. Muscle regeneration is, at least in part, regulated by caspase activation, and AVP abrogated TNF-dependent caspase activation. The contrasting effects of AVP and TNF in vivo are recapitulated in myogenic cell cultures, which express both PW1, a caspase activator, and Hsp70, a caspase inhibitor. We identified PW1 as a potential Hsp70 partner by screening for proteins interacting with PW1. Hsp70 and PW1 co-immunoprecipitated and co-localized in muscle cells. In vivo Hsp70 protein level was upregulated by AVP, and Hsp70 overexpression counteracted the TNF block of muscle regeneration. Our results show that AVP counteracts the effects of TNF through cross-talk at the Hsp70 level. Therefore, muscle regeneration, both in the absence and in the presence of cytokines may be enhanced by increasing Hsp70 expression. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
45. Skeletal muscle is enriched in hematopoietic stem cells and not inflammatory cells in cachectic mice.
- Author
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Berardi, Emanuele, Aulino, Paola, Murfuni, Ivana, Toschi, Angelica, Padula, Fabrizio, Scicchitano, Bianca M., Coletti, Dario, and Adamo, Sergio
- Abstract
Objective: Cachexia, a debilitating syndrome characterized by skeletal muscle wasting, is associated to many chronic diseases and diminishes the quality of life and survival of patients. Tumor-derived factors and proinflammatory cytokines, including TNF-alpha, IL-6 and IL-1beta, mediate cachexia. In response to elevated cytokine levels, increased proteasome-mediated proteolysis and auto-phagocytosis result in muscle wasting. The histologic features of muscle cachexia are not fully elucidated. Therefore, we analysed alterations of different cell populations in cachectic muscle. Methods: By immunohistochemical and cytological approaches, we characterized changes in the abundance of cellular populations in the musculature of a murine model of cancer cachexia (C26-bearing mice). Results: Cachectic muscle displayed a decreased DNA content proportional to muscle mass wastage. A decrease in the number of nuclei occurred in the muscular but not in the stromal compartment. Cachectic muscle showed: mild modulation of myeloperoxidase activity, a neutrophil marker; reduction of macrophages in the endomysium; decrease in CD3
+ lymphocyte number. Conversely, a statistically significant enrichment in Sca-1+ CD45+ hematopoietic stem cells (HSCs) occurred in cachectic muscle. Discussion: The elevated levels of cytokines which characterize cachexia may represent a trigger for inflammatory cell activation. However, we find that in cachexia, inflammatory cells in muscle are not increased while muscle tissue nuclei decline. Our data suggest that the inflammatory cell-mediated stress is not an etiologic component of muscle wasting in cachexia. The relative increase in HSCs in cachectic skeletal muscle suggests an attempt to maintain muscle homeostasis by recruitment and/or activation of stem cells. [ABSTRACT FROM AUTHOR]- Published
- 2008
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- View/download PDF
46. Increase in cytosolic Ca[sup 2+] induced by elevation of extracellular Ca[sup 2+] in skeletal myogenic cells.
- Author
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Naro, Fabio, De Arcangelis, Vania, Coletti, Dario, Molinaro, Mario, Zani, Bianca, Vassanelli, Stefano, Reggiani, Carlo, Teti, Anna, and Adamo, Sergio
- Subjects
CELL membranes ,MYOBLASTS ,CYTOPLASM - Abstract
Cytoplasmic Ca[sup 2+] concentration ([Ca[sup 2+]][sub i]) variation is a key event in myoblast differentiation, but the mechanism by which it occurs is still debated. Here we show that increases of extracellular Ca[sup 2+] concentration ([Ca[sup 2+]][sub o]) produced membrane hyperpolarization and a concentrationdependent increase of [Ca[sup 2+]][sub i] due to Ca[sup 2+] influx across the plasma membrane. Responses were not related to inositol phosphate turnover and Ca[sup 2+]-sensing receptor. [Ca[sup 2+]][sub o]-induced [Ca[sup 2+]][sub i] increase was inhibited by Ca[sup 2+] channel inhibitors and appeared to be modulated by several kinase activities. [Ca[sup 2+]][sub i] increase was potentiated by depletion of intracellular Ca[sup 2+] stores and depressed by inactivation of the Na[sup +]/Ca[sup 2+] exchanger. The response to arginine vasopressin (AVP), which induces inositol 1,4,5-trisphosphate-dependent [Ca[sup 2+]][sub i] increase in L6-C5 cells, was not modified by high [Ca[sup 2+]][sub o]. On the contrary, AVP potentiated the [Ca[sup 2+]][sub i] increase in the presence of elevated [Ca[sup 2+]][sub o]. Other clones of the L6 line as well as the rhabdomyosarcoma RD cell line and the satellite cell-derived C2-C12 line expressed similar responses to high [Ca[sup 2+]][sub o], and the amplitude of the responses was correlated with the myogenic potential of the cells. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
47. TNFa inhibits skeletal myogenesis through a PW1-dependent pathway by recruitment of caspase pathways.
- Author
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Coletti, Dario, Yang, Ellen, Marazzi, Giovanna, and Sassoon, David
- Subjects
- *
TUMOR necrosis factors , *MYOGENESIS , *CACHEXIA , *MUSCLES , *CELL death , *MITOCHONDRIA - Abstract
Cachexia is associated with poor prognosis in patients with chronic disease. Tumor necrosis factor-alpha (TNFα) plays a pivotal role in mediating cachexia and has been demonstrated to inhibit skeletal muscle differentiation in vitro. It has been proposed that TNFα- mediated activation of NEκ leads to down regulation of MyoD, however the mechanisms underlying TNFα effects on skeletal muscle remain poorly understood. We report here a novel pathway by which TNEα inhibits muscle differentiation through activation of caspases in the absence of apoptosis. TNFα-mediated caspase activation and block of differentiation are dependent upon the expression of PW1, but occur independently of NEκB activation. PW1 has been implicated previously in p53-mediated cell death and can induce bax transtocation to the mitochondria. We show that bax-deficient myoblasts do not activate caspases and differentiate in the presence of TNFα, high- lighting a rote for bax-dependent caspase activation in mediating TNFα effects. Taken together, our data reveal that TNFα inhibits myogenesis by recruiting components of apoptotic pathways through PW1. [ABSTRACT FROM AUTHOR]
- Published
- 2002
- Full Text
- View/download PDF
48. NMRK2 Gene Is Upregulated in Dilated Cardiomyopathy and Required for Cardiac Function and NAD Levels during Aging.
- Author
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Tannous, Cynthia, Deloux, Robin, Karoui, Ahmed, Mougenot, Nathalie, Burkin, Dean, Blanc, Jocelyne, Coletti, Dario, Lavery, Gareth, Li, Zhenlin, Mericskay, Mathias, and Lymperopoulos, Anastasios
- Subjects
DILATED cardiomyopathy ,MOLECULES ,CARRIER proteins ,CARDIAC hypertrophy ,MIDDLE age - Abstract
Dilated cardiomyopathy (DCM) is a disease of multifactorial etiologies, the risk of which is increased by male sex and age. There are few therapeutic options for patients with DCM who would benefit from identification of common targetable pathways. We used bioinformatics to identify the Nmrk2 gene involved in nicotinamide adenine dinucleotde (NAD) coenzyme biosynthesis as activated in different mouse models and in hearts of human patients with DCM while the Nampt gene controlling a parallel pathway is repressed. A short NMRK2 protein isoform is also known as muscle integrin binding protein (MIBP) binding the α7β1 integrin complex. We investigated the cardiac phenotype of Nmrk2-KO mice to establish its role in cardiac remodeling and function. Young Nmrk2-KO mice developed an eccentric type of cardiac hypertrophy in response to pressure overload rather than the concentric hypertrophy observed in controls. Nmrk2-KO mice developed a progressive DCM-like phenotype with aging, associating eccentric remodeling of the left ventricle and a decline in ejection fraction and showed a reduction in myocardial NAD levels at 24 months. In agreement with involvement of NMRK2 in integrin signaling, we observed a defect in laminin deposition in the basal lamina of cardiomyocytes leading to increased fibrosis at middle age. The α7 integrin was repressed at both transcript and protein level at 24 months. Nmrk2 gene is required to preserve cardiac structure and function, and becomes an important component of the NAD biosynthetic pathways during aging. Molecular characterization of compounds modulating this pathway may have therapeutic potential. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
49. Inflammation in Muscle Repair, Aging, and Myopathies.
- Author
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Bouché, Marina, Muñoz-Cánoves, Pura, Rossi, Fabio, and Coletti, Dario
- Published
- 2014
- Full Text
- View/download PDF
50. Mesenchymal Stem Cells as Promoters, Enhancers, and Playmakers of the Translational Regenerative Medicine 2018.
- Author
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Ballini, Andrea, Cantore, Stefania, Scacco, Salvatore, Coletti, Dario, and Tatullo, Marco
- Subjects
MESENCHYMAL stem cells ,REGENERATIVE medicine - Published
- 2018
- Full Text
- View/download PDF
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