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目的探讨无创产前检测（NIPT）提示18-三体高风险胎儿的罕见异常核型起源及对生育的影响。方法产前诊断一例罕见完全性易位型18-三体病例，结合细胞及分子遗传学分析对胎儿染色体异常进行溯源。以“易位型18-三体”、“18-三体易位型”（包括中、英文）为检索词，对PubMed、CNKI、SinoMed文献服务系统、万方数据知识服务平台、维普中文科技期刊数据库及中华医学期刊全文数据库进行检索，收集并分析检索到的病例资料。结果胎儿SNP array提示18-三体，经父母G-显带核型分析验证，孕妇外周血染色体核型为46，XX，t（9；18）（q31.2；q23），胎儿最终确认核型为47，XN，t（9；18）（q31.2；q23）mat，+18，为罕见的完全性易位型18-三体；与父母SNP array溯源分析显示胎儿有2份完整18号染色体遗传物质来自染色体平衡易位的母亲。文献检索发现国外报道2例完全性易位型18-三体患儿，均有18-三体表型且都来源于亲本18号染色体与其他染色体的平衡易位。结论NIPT能有效提前预警或预诊18-三体；SNP array技术不仅能提高染色体异常的检出率，还可以对异常染色体进行溯源；胎儿细胞染色体核型分析仍是产前诊断染色体异常的金标准。

光纤环是光纤陀螺的传感核心,如何绕制高质量的光纤环,对光纤陀螺的研制十分重要,本文从理论和实践两方面说明光纤环的绕制。理论和实践一致,方法简单易行。

【目的】 研究清开灵注射液(QKL)对小鼠T细胞体外活化65380;增殖65380;细胞周期和迟发型超敏反应(DTH)的影响,探讨其免疫调节作用及机制65377;【方法】 不同浓度的QKL作用于小鼠淋巴细胞4 h后,加入刀豆蛋白A (ConA)刺激,于不同时间点收获细胞,利用流式细胞仪检测T细胞的活化65380;增殖和细胞周期分布情况;将小鼠分为正常对照组65380;二硝基氟苯(DNFB)诱导的DTH模型组和QKL治疗组,通过分析小鼠脾脏指数和耳朵肿胀率评价QKL对DTH的影响65377;【结果】 ConA刺激12 h后,T细胞表面CD69表达率为63.1% ± 1.3%,QKL下调CD69的表达,加入浓度为1/128065380;1/320和1/80的QKL后,CD69表达率依次降为56.2% ± 2.4%65380;51.9% ± 2.8%和39.1% ± 1.4%,与ConA对照相比,差异有统计学意义(P < 0.05);各浓度QKL明显降低ConA刺激的细胞增殖指数,且呈一定的浓度依赖性65377;对细胞周期分布的影响表现为:QKL增加ConA激活的细胞中处于S期和G2/M期的细胞数目,降低处于G0/G1期的细胞数目;体内应用QKL治疗小鼠DTH的结果为:QKL治疗组小鼠脾脏指数为4.96 ± 0.90,与模型组(脾脏指数为5.78 ± 0.85)相比, P < 0.05; 发病耳的肿胀率为28.4% ± 4.2%,与模型组(肿胀率为42.4% ± 4.6%)相比,P < 0.0165377;【结论】 QKL是一种有效的负向免疫调节剂,能抑制体内外的免疫反应,抑制T细胞的活化和增殖可能是其作用机制之一65377;

【目的】 建立适于人表皮组织的双向电泳技术,并应用质谱技术对人表皮组织蛋白质组进行初步分析?【方法】 利用组织匀浆法制备人表皮组织总蛋白?固相pH 梯度胶条进行双向电泳?PDQuest 7.4软件比较和分析电泳图谱,利用质谱技术鉴定部分蛋白斑点?【结果】 成功建立了适用于人表皮组织蛋白质组分析的双向电泳技术,获得和比较了人表皮组织双向电泳四种染色图谱?人表皮组织的蛋白质在pH5～8范围内分布均匀,分子量集中在15～100 ku之间?银染图谱获得的蛋白斑点数为586±14;对质谱兼容的几种染色方法进行比较分析,结果表明Neuhoff 胶体考染更适合于人表皮组织蛋白质的双向电泳分析,其在上样量为350 μg时蛋白斑点数达394±9,匹配率为85.53%?利用基质辅助激光解析电离飞行时间质谱技术成功获得了Neuhoff 胶体考染的两个蛋白斑点的肽质量指纹图谱,并鉴定为Caspase 14 前体和27 ku热激蛋白1?【结论】建立的表皮组织双向电泳技术及其图谱可为皮肤蛋白质组学研究提供参考,为人皮肤蛋白质组学平台的构建奠定良好基础?

[目的]研究人类免疫缺陷病毒（HIV）-1 nef调节细胞表面的人类白细胞抗原（HLA）-Ⅰ类分子及诱导细胞凋亡情况。[方法]将293T细胞分为两组,用脂质体法将含HIV-1 nef基因的质粒及空载体分别转染细胞,Western blot检测HIV-1 nef在转染细胞中的表达,荧光抗体染色结合流式细胞术检测细胞表面的HLA-I类分子表达及细胞凋亡情况。[结果]HIV-1 nef在转染细胞中有表达。转染HIV-1 nef的细胞与转染空载体的细胞经抗HLA-A,B,C-FITC染色后,平均荧光强度分别为205±22及269±15,两者相比有显著性差异（P〈0.01）;经Annexin V-APC/PI染色后,阳性细胞百分率分别为（60.82±8.32）%及（8.12±5.43）%,两者相比亦有显著性差异（P〈0.01）。[结论]HIV-1 nef下调细胞表面的HLA-I类分子及诱导细胞凋亡。

This article reviewed the nursing management and related nosocomial infection prevention and control measures for a diabetic patient with Norwegian scabies. Given the strong infectiousness of Norwegian scabies, it is required to activate the contingency plan for nosocomial outbreak prevention and control. Hospital isolation and standard precautions were conducted immediately after diagnosis. An enhanced specialist care was carried out, including medication care, skin care, psychological care and social support. (本文总结了1例糖尿病合并挪威疥疮患者的护理经验及医院感染防控措施和。挪威疥疮传染性强, 应立即启动医院感染暴发应急预案, 做好隔离消杀措施, 医护人员实施标准预防, 患者专人集中护理, 包括用药护理、皮肤护理及心理护理, 同时加强支持与鼓励, 提高患者治疗依从性。)

本文叙述PFM彩色电视双伴音传输设备的主要性能和应用。该设备在接收灵敏度为-34.5dBm时,仍然满足广播电影电视部部标GY—38—86短程光缆传输规定的甲级指标。

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Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To Investigate the effect of copper ions on the radiosensitivity of nasopharyngeal carcinoma （NPC） and identify potential targets to improve the efficacy of NPC radiation therapy. Methods The content of copper ions in normal nasopharyngeal epithelial cell line NP69 and a variety of NPC cell lines was detected by a copper microplate assay. After adding 0，5，10，20，50，100，200 μmol/L copper ion solution and 0，0.05， 0.1，0.2，0.5，1.0，2.0 mmol/L TEPA solution， CCK⁃8 assay was used to determine the effect of intracellular copper ion content on the survival rate of NPC cells before and after irradiation. And determine the drug concentration to be used in subsequent experiments. CCK⁃8 assay was used to determine the effect of intracellular copper ion content on the survival rate of NPC cells before and after irradiation， and to determine the drug concentration used in the copper ion group and TEPA group in the subsequent experiments. CCK⁃ 8 assay and clone formation assay were used to detect the changes in radiosensitivity of NPC cells after drug treatment. The DNA damage and MAPK-ERK signaling pathway-related protein expression of NPC cells in each group before and after irradiation were detected by Western blot. Results The content of copper ions in NPC cells was significantly higher than that in normal nasopharyngeal epithelial cells （P < 0.05）. When the concentration of copper ion solution was lower than 50 μmol/L， the radiation resistance of CNE1 cells was improved， and the radiation sensitivity of SUNE1 cells was increased by 0.1 ~ 0.2 mmol/L TEPA （P < 0.05）. Compared with the control group， the NPC cell radiotherapy resistance of copper ion group was enhanced， and the NPC cell radiotherapy sensitivity of copper ion group was enhanced （P < 0.05）. Compared with the control group， the activation degree of MAPK-ERK pathway in copper ion group was up-regulated （P < 0.05）. MAPK-ERK pathway inhibitor SCH772984 effectively reversed copper-mediated NPC radiotherapy resistance （P < 0.05）. Conclusion The increased copper ion content in NPC cells promoted the radiotherapy resistance of NPC cells by activating the MAPK-ERK signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Nanjing Medical University: Natural Sciences is the property of Journal of Nanjing Medicial University (NMU) Medical Publications Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effect of nutritional status before the first chemotherapy on the tolerance, efficacy, adverse reactions and survival of patients with advanced gastric cancer. Methods A total of 336 patients with advanced gastric cancer admitted to Fujian Cancer Hospital from May 2018 to July 2020 were selected to evaluate the nutritional status before the first chemotherapy by the Global Leadership Initiative on Malnutrition(GLIM). The patients were divided into the malnutrition group(116 cases) and the wellnourished group(220 cases) according to nutritional status. The relationship between malnutrition and clinical outcome in the patients with advanced gastric cancer was evaluated by using the propensity score matching method to balance the inter-group confounding factors. Results Among 336 patients with gastric cancer, 116 (34. 5%) had malnutrition. Before matching, there were significant differences in age and tumor size between the two groups(P >0. 05). The 103 pairs were successfully matched by 1∶1 nearest neighbor matching method. After matching, the baseline data between the two groups were balanced. The gastrointestinal reaction, neuropathy and fatigue degree of the malnutrition group were higher than those of the well-nourished group (P <0. 05). The chemotherapy tolerance, curative effect and survival time of the malnutrition group were lower than those of the well-nourished group(P <0. 05). Conclusion The incidence of malnutrition in the patients with advanced gastric cancer is high, which increases the adverse reaction of chemotherapy and reduces the tolerance and survival time of chemotherapy. [ABSTRACT FROM AUTHOR]

【Objective】Drought is one of the main abiotic factors that affect rice productivity. Rice yield can be ensured by screening drought-resistant rice.【Method】Ninety-three single-segment substitution lines (SSSLs) constructed from Oryza. meridionalis and O. glumaepatula and their recipient parents ‘Huajingxian 74’(HJX74) were used as the test materials. And 20% PEG-6000 was adopted to simulate drought conditions to perform drought tolerance tests at the seedling stage. Besides, four growth and development traits (relative seedling height, relative seedling dry weight, relative root dry weight and relative root length) were used as evaluation indicators. The membership function method was utilized to sort SSSLs and screen SSSLs with relevant traits significantly different from HJX74. Based on the QTL identification and additive effect analysis, QTL related to drought tolerance traits at the seedling stage was preliminarily identified.【Result】The analysis of correlation between the four growth and development traits and the average membership function value revealed that they were all extremely significantly positively correlated, and could be used as indicators for identifying and evaluating the drought tolerance in the seedling stage of SSSLs. According to the analysis result of the membership function method, the average membership function value of the tested SSSLs ranged from 0.20 to 0.71, and the average membership function value of HJX74 was 0.54. The average membership function value of 29 SSSLs was greater than that of HJX74. Among them, M124 had the largest membership function value, indicating strong drought tolerance of M124. Through single factor analysis of variance, 7 relative seedling height QTLs (qRSH1-1, qRSH1-2, qRSH2-1, qRSH3-1, qRSH3-2, qRSH5-1, and qRSH6-1) were identified from 8 SSSLs (M78-1, M78-2, M124, M107, M151, M103, M130, M115), which were distributed on Chromosomes 1, 2, 3, 5, and 6. Their additive effect was 0.05-0.06, and the phenotypic contribution rate was 6.97%-9.01%. Three relative root dry weight QTLs (qRRDW10-1, qRRDW11-1, and qRRDW11-2) were identified from three SSSLs (M79, M145, M148), which were distributed on chromosomes 10 and 11. The additive effect was 0.12-0.20, and the phenotypic contribution rate was 12.51%-19.95%. A relative seedling dry weight QTL (qRSDW5-1) was identified from 2 drought-tolerant SSSLs (M80, X149), and distributed on chromosome 5, with the additive effect of 0.07 and the phenotypic contribution rate of 10.5%.【Conclusion】A total of 13 SSSLs with droughttolerant QTLs at the seedling stage are screened, laying a solid foundation for further study on fine mapping and cloning. [ABSTRACT FROM AUTHOR]

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The forage silage can not only prevent the loss of nutrients, but also reduce the volume of feed and solve the problem that the forage grass is difficult to preserve. However, many grass have the problems of high water and low sugar content. Thus, the quality of direct silage is lower. Additives can improve the quality of silage and the commonly used silage additives include enzyme and lactic acid bacteria preparation. How to play the advantages of different silage additives and improve the quality of silage are crucial to increase the utilization rate of forage grass. This review paper introduced the effects characteristics of silage additives, concluded the effects of additives on fermentation quality, nutritional value and aerobic stability of forage silage, and emphatically summarized the effects of additives on microbial diversity of forage silage. [ABSTRACT FROM AUTHOR]

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Saving energy resources and reducing energy intensity are crucial to promoting China' s major strategic goals of reaching peak carbon emissions and achieving carbon neutrality, and are hence key to the promotion of green transformation and development in the new development stage. As an important practice, the policy of establishing pilot zones for green finance reform and innovations in China aims to promote green finance reform in depth, construct ecological civilization, and realize green economic transformation. Can the policy reduce energy intensity and contribute to energy conservation? What is its impact mechanism? There is a shortage of systematic analysis in academia on these issues. With panel data from Chinese cities from 2007 to 2020 and a generalized synthetic control method, this paper evaluated how the establishment of pilot zones for green finance reform and innovations affected energy intensity and analyzed its impact mechanisms. The results showed that: ① The implementation of the policy significantly reduced the energy intensity of the pilot zones, and the energy-saving effect exhibited 'policy first' and timeliness characteristics. ② The policy-induced energysaving effect was achieved by improving green innovation, easing financing constraints, optimizing industrial structure, and strengthening environmental governance. ③ The policy' s energy-saving effect was more remarkable in areas with better marketization and a higher education level. The findings suggest that the government should fully consider the differences in aspects such as institutional environment and resource endowment among varying regions, design distinct measures to attract more market players to participate in the green finance system oriented by energy efficiency improvement, energy structure transformation, and environmental governance, and create a much better market-oriented environment for green innovation and corporate financing. [ABSTRACT FROM AUTHOR]

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