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Objective To construct a recombinant retroviral vector containing human ZAK-β cDNA. Methods ZAK-β cDNA was amplified from lung cancer A459 cells by RT-PCR with ZAK-β-specific primers. PCR product was cloned into eukaryotic expression vector pMSCV-IRES-GFP, and then sequenced. Results ZAK-β cDNA was successfully amplified by RT-PCR. Expression vector pMSCV-IRES-GFP-ZAK-β was also successfully obtained and confirmed. Conclusion The recombinant retroviral vector bearing human ZAK-β cDNA was successfully constructed, which forms the basis for the further studies on ZAK-β function. [ABSTRACT FROM AUTHOR]