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This work aims to study the case of the stained glass window at the Royal Palace in Edfena, Egypt. The research also aims to identify the chemical composition and diagnose the manifestations of damage on the stained glass window. Therefore, the examination and analytical study were conducted using several different techniques, including AutoCAD, digital and stereo microscopies, scanning electron microscope - X-ray energy dispersion, X-ray diffraction, and microbiological examination. The microscopic study revealed that the surface of the painted glass suffers from the presence of layers of damaged products, which obscure the decorations and lead to opacity. Light holes spread across the layers of different paints with varying sizes, and pits appear as a result of corrosion. Using EDX, the element responsible for the color in the painted glass layer was determined, as well as the composition of the lead came. XRD was used to identify the crystalline compounds in the painted glass layer and the putty composition. In the end, an appropriate treatment plan was drawn up for the condition of the lead-stained glass window to preserve it from loss and damage due to its archaeological and artistic value. [ABSTRACT FROM AUTHOR]

The blowfly, Lucilia cuprina (Diptera: Calliphoridae) is an important medical and forensic insect that is the main cause of ovine cutaneous myiasis. In the field of medical treatment using maggot therapy, this species could help to heal incurable infections. The morphological identification of the adult wing and Scanning Electron Microscope (SEM) of the first instar larvae was carried out and confirmed by DNA identification of the 3rd instar larvae of L. cuprina. The results showed a fraction of the mitochondrial cytochrome oxidase I (COI) spanning approximately 269 bp. The topology of the Neighbor-Joining (NJ), Maximum Likelihood (ML) tree indicated that L. cuprina species were assigned correctly to the subfamily Luciliinae, family Calliphoridae. The male reproductive system comprises two testes, a pair of vasa differentia and a pair of accessory glands, a vesicula seminalis, and an ejaculatory duct. This inquired species has the common conventional spermatozoal pattern of Calliphoridae, a single-layered acrosome at the apex, a consolidated nucleus, totally crystallized mitochondrial derivatives, and an axoneme with a 9+9+2 microtubular configuration. This investigation revealed for the first time detailed morphological and mitochondrial DNA identification, as well as the morphology of the male reproductive system and ultrastructure of spermatozoa in L. cuprina, collected from Sharkia Governorate, Egypt. [ABSTRACT FROM AUTHOR]

The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA, decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm. Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn't show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. It recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases, instead of copper pesticides. It could be a new, approach for disease management and increase the plant's nutritional value. [ABSTRACT FROM AUTHOR]

Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae) is a parasitoid to several stored product beetles. In this study, this parasitoid was recorded for the first time with density on the cowpea beetle Callosobruchus chinensis L, which was prevalent on both Egyptian bean (Vicia faba L) and cowpea bean (Vigna unguiculata). A full description of this parasitoid and measurements of its body parts were done using a scanning electron microscope. The male and female were differentiated based on the antenna and a set of taxonomic and diagnostic characteristics were found. The current study provides basic information on the external morphology key identification, characteristics and measurements of taxonomically significant portions, backed by micrographs taken with a scanning electron microscope. This description can be used to include them in the taxonomic keys to differentiate them from closely similar species. The present study was carried out at the Atomic Energy Center in Egypt during field observation in small grain stores of cowpea and faba beans where the effectiveness of the parasite to control cowpea beetle, in particular, was observed. This parasitoid was the first time found on cowpea beetle in a high density, which necessitated carrying out an extensive morphological study of A. calandrae in order to identify it for future breeding. Additionally, it can be in future widely used in the biological management of pests that attack stored grains in general, and C. chinensis in particular. [ABSTRACT FROM AUTHOR]

Microbial interactions are frequently categorized according to how they affect each population in a binary system. We aimed to determine the interaction between P . aeruginosa, S . aureus, and E . coli in-vitro. In this experimental hospitalized patients' sputum, urine, and blood samples were used to collect a total of 90 clinical isolates for the study in Damanhour Medical National Institute, Behira, Egypt, followed by accurate identification and testing for antibiotic sensitivity. To examine the effect of the supernatant of P. aeruginosa on S. aureus and E. coli determined MIC using broth microdilution method. We also measured the activity of lasA protease by assessing the S. aureus cell lysis potential of P. aeruginosa culture supernatants. Extraction of pyocyanin was made to determine the change in the cell nature of S. aureus upon exposure to pyocyanin by using a scanning electron microscope and the shape of colonies on the culture media was determined. Finally, we detect lasA, operon phz, phzM, phzS and rhlAB genes for P. aeruginosa. P. aeruginosa showed a great impact on S. aureus isolates resistant to different antibiotics as it facilitates their killing and may drive the normal colonies of S. aureus into SCVs. The ability to form biofilm by S. aureus and E. coli decreased in the presence of Pseudomonas supernatant. [ABSTRACT FROM AUTHOR]

Gnathostomiasis is a fish-borne zoonotic infection caused by eating infected seafood or fish parasitized by third-stage nematode larvae of the genus Gnathostoma. Twenty-seven fish specimens of the European eel Anguilla anguilla (Anguillidae) were collected from water coasts of the Mediterranean Sea in Egypt. Of the 27 fish that were studied, 14 fish (51.58%) were parasitized with third-stage larvae (L3) of Gnathostoma sp. (Gnathostomatidae), these larvae were investigated as encapsulated on the surface of different visceral organs and some embedded in muscles of the infected fish. By using both light and scanning electron microscopes, the morphological examination of the recovered nematodes revealed that the main diagnostic features represented by an elongated body, measured 6.5±2 (7.8-9) mm long, and its greatest width observed at the posterior one-third of the body was 0.4±0.02 (0.38-0.44) mm with narrow lateral alae, the presence of a cephalic bulb measured 0.26±0.02 (0.18-0.28) mm long, 0.31±0.02 (0.28-0.34) wide and armed with six transverse rows of spines, mouth opening surrounded by two simple lateral lips, and conical tail terminated with a pointed mucron. The described species was morphologically and morphometrically compared with some of the previously recorded species of the same genus. Based on our detailed description, the present species was identified as Gnathostoma sp. recovered from A.anguilla as the first record in Egypt. [ABSTRACT FROM AUTHOR]

The paper aims to study a rare Roman glass cup, consisting of a set of glass breakages, preserved at the Museum of Archeology Department - College of Tourism and Archaeology, King Saud University, Saudi Arabia. The cup was found at Qaryat Al-Faw site (also Qaryat Al-Fau), the capital of the first Kindah kingdom (fourth century BC-fourth century AD). It is located about 100 km south of Wadi ad-Dawasir, and about 700 km southwest of Riyadh, the capital city of Saudi Arabia. Qaryat Al-Faw archeological site reveals various features such as residential area, markets, roads, cemeteries, temples, and water wells. On the exterior surface of the glass cup, there are color graphics representing what seems to be Egyptian(zing) elements. This cup was the only one consisting of such elements in all sites cross the Arabian Peninsula. Scanning Electron Microscope and Differential Scanning Calorimetry were applied indicating soda glass type, and compared with the chemical analysis of con-temporary imported pieces from Egypt the present study indicated that no furnaces for the manufacture of glass were found in Qaryat Al-Faw site, which promotes the import hypothesis. According to preliminary interpretations, it is likely that the cup was of a Roman origin and was probably made in Alexandria in Egypt and dates from about the late first-early second century AD, and apparently brought to the site by one of Qaryat Al-Faw merchants. Although Qaryat Al-Faw is also famous of glass remains, there are no information about any local production of glass in the site and no kilns had been found. The local glass industry at Qaryat Al-Faw site, if any, was not documented during archaeological excavations conducted by the Dept of Archaeology, King Saud University. [ABSTRACT FROM AUTHOR]

This paper studies some ceramic lustreware dishes with metallic glaze dating back to the Abbasid era (2-3rd century AH/8-9th AD) and preserved in Rashid archaeological stores. The samples were selected based on the diversity of textual and pictoral decorations including written words, plants, animals and geometric elements. The manufacturing process and mineral and chemical composition of the dishes have been subjected to several examination and analytical methods including stereo microscope, scanning electron microscope (SEM-EDX), X-ray diffraction (XRD), X-ray fluorescence (XRF), Fourier transform infrared (ATR-FTIR) spectroscopy and differential thermal analysis (DTA). The study revealed a heterogeneous distribution of the color grains on the surface of the dishes and nanoparticles of metallic silver/copper ranging between 50-100nm. The metal oxides of the silver nanoparticles appeared in high percentage compared to nano copper particles on the surface of the lustre metallic layer which fact accounts for the greenish brown color of the dishes. While Arabic gum was used for fixing the decorations, the lustre layer was fired at 500°C and the firing of the ceramic body varied between 700°C and 800°C. A treatment and maintenance plan is suggested to preserve the pieces from further damage owing to their high archaeological value. [ABSTRACT FROM AUTHOR]

This paper aims to evaluate of silica and alumina nanocomposites in consolidation and protection of archaeological glass from early Islamic period in Egypt. Archaeological glass is exposed to deterioration by moisture, as water is considered one of the most dangerous damage factors that attack glass artifacts. This results in many aspects of deterioration including weeping glass, cracks, dulling, formation of weathering crusts, salt growing, and corroded surfaces. From this standpoint, materials which are used in the consolidation and protection of those glass artifacts must have the property of hydrophobicity in order to protect them from the harmful effects of water. In the last decade, polymer-nanoparticle composites have attracted great interest in the field of culture heritage conservation due to their unique multifunctional properties resulting from the high surface area and chemical activity of the nanoparticles dispersed in the polymers. In this study, Silicon dioxide (SiO2) and alumina oxide (Al2O3) nanoparticles are added to silicon based polymer (poly alkyl siloxane) (Silres BS-290) in order to improve their physiochemical and mechanical properties. This produced a significant improvement in the ability of the polymers to consolidate and protect archaeological glass. The treated samples are exposed to artificial ageing to test the efficiency of the consolidation materials. The properties of untreated, treated and aged glass samples were evaluated by TEM, stereo microscope, scanning electron microscope (SEM), colormetric measurements, static water contact angle, density and hardness tests (physical and mechanical properities). Results showed that the treated sample with SiO2/Al2O3/Poly siloxane nanocomposites are good candidates for consolidation and protection of glass objects. [ABSTRACT FROM AUTHOR]

Scorpions are terrestrial arthropods inhabiting deserts. Exomorghological studies play an important role in the identification of different scorpions' species. The current study deals with the ecomorphology of four species of scorpions belong to the family buthidae (Leiurus quinquestriatus, Androctonus amoreuxi, Orthochirus innesi and Buthacus leptochelys) inhabiting New Valley governorate, Egypt. Random samples were implemented during the period from April to July, 2017 and July to September, 2019. Scorpions were obtained from the area under rocks, gap of soil, leaf litter, under bark, and within vegetation. Morphological characteristics of these species were done using a scanning electron microscope (SEM). In conclusion, the differences among four species of studied scorpions were noticed in the dorsal carapace, respiratory pores of the third segment of mesosoma, pectinal teeth count between females and males, last segment of metasoma and telson. In all scorpions' species studied, one venom pore was detected except in Androctonus amoreuxi two venom pores in telson were observed. [ABSTRACT FROM AUTHOR]

Nutlets of 13 taxa of subfamily Cyperoideae (Cyperaceae) from Egypt were investigated using light and scanning electron microscopes to ascertain the taxonomic utility of the nutlet in corroborating and elaborating on distinctions between closely related genera and species. The studied taxa included representatives of tribes Fuireneae, Cypereae, Cariceae, Abildgaardieae and Schoeneae. Nutlet shape, size, color, surface, epidermal cell shape, anticlinal boundaries, outer periclinal cell wall and relief of outer cell walls were provided. It was found that the epidermis has useful microscopic characters to help in the taxonomic assessment of Cyperoideae. Seven different types of anticlinal cell wall boundaries were described, four types of outer periclinal cell walls, and five different forms of the secondary cell walls were recognized. A diagnostic key for the studied taxa based on nutlet characters was presented. The results showed congruence between the UPGMA dendrogram and principal component analysis in suggesting two major groups and six subgroups. The most important characters with the highest factor loadings along the first two PCA axes included nutlet shape, nutlet apex, surface topography and the presence of silica bodies. The results of this study confirmed the heterogeneity of the Cyperoideae and indicated that Fuireneae is the most heterogeneous tribe. [ABSTRACT FROM AUTHOR]

The microstructures of the carapaces of the spider crabs Schizophrys dahlak and Hyastenus hilgendorfi were examined under both scanning and transmission electron microscopes. Image analyses of the cross-sectioned carapaces showed that both species have a hierarchical microstructure with three well-defined layers. The two innermost layers (exocuticle and endocuticle) consist of stacked fibers arranged in a twisted plywood fashion producing a "Bouligand structure". They have a similar structure but different thicknesses and height of the stacks. The cuticles are inter-stitched with pore canals of varying width between the studied species, running transversely to the layered structure. This variation in the width of the pore canals in the two species seems to be proportional to the size of the carapace and its surface area. [ABSTRACT FROM AUTHOR]

The local entomopathogenic bacterium, Bacillus thuringiensis (Bt) was isolated and characterized from 16 soil samples collected from different governorates in Egypt. Among 56 bacterial colonies obtained, only 16 colonies were characterized by traits of Bacillus. All the 16 isolates were toxic to the neonates of the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Damietta and El-Sharkyia bacterial isolates showed appreciable mortality rates (100 and 96.6%), respectively, which were higher than that caused by the standard isolates of Bt entomocidus (that produce Cry1 C toxin) for which they were selected for further characterization. Scanning electron microscope of Damietta bacterial isolate showed the presence of a bipyramidal crystal protein; consistent with the presence of Cry1 toxin class, however, El-Sharkyia bacterial isolate produced spherical-shaped crystals consistent of Cry2 toxin class. Electrophoretic patterns of different isolates and standards revealed different molecular weight bands, ranged from 195 to 8KDa. Damietta and El-Sharkyia bacterial isolates produced major protein bands with molecular weights of 130 KDa, which was also present in Bt entomocidus. Both isolates also shared protein bands with Bt entomocidus with molecular weights of 80, 70, 65, 51, and 22 KDa. The 16S rRNA sequences of both isolates were submitted to the NCBI Gene Bank database, with accession numbers of LC070660 for Damietta isolate and LC070661 for El-Sharkiya isolate. The existence of different Cry genes in the 2 isolates was studied by PCR, using general primers of 5 Cry genes. Cry1 gene was detected in both isolates; however, Cry 2 gene was detected only in Damietta isolate. [ABSTRACT FROM AUTHOR]

Camel abscess is one of the important diseases that can be caused by pyogenic bacteria. Abscess were mainly reported on the dromedary camel of the Nile Delta of Egypt, but rarely reported on Bactrian camel. The purpose of this study is to identify the pathogens isolated from the samples of camel abscess. The scanning electron microscope was applied for pathogenic morphological study. The 16S rRNA genes amplification and sequencing, and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) method were employed to identify the bacteria. In addition, drug sensitivity tests were performed for the potential prevention and control of the disease. The results showed that two bacterial strains of Staphylococcus were isolated. Amplification of 16S rRNA and construction of phylogenetic tree demonstrated that they were Staphylococcus aureus (S. aureus) and Staphylococcus sciuri (S. sciuri). The results of MALDI-TOF MS confirmed that the causative bacteria causing camel abscess were S. aureus and S. sciuri. Drug sensitivity tests showed that S. aureus was resistant to Amoxicillin, Penicillin G, Erythromycin, Linezolid, Clindamycin, and Chloramphenicol, and was moderately resistant to Rifampicin, Doxycycline, and Tetracycline, but was sensitive to seven other antibiotics. S. sciuri was resistant to Cefotaxime, but sensitive to fifteen other antibiotics. This study is the first report on S. sciuri infected camels and provides a scientific basis for selecting antibiotics that are effective for treating camel abscess. [ABSTRACT FROM AUTHOR]

The present work introduces a laboratory multi-technique study for a unique heritage object from the Persian Safavid art preserved in Egypt. This study is alleged to be the preliminary one of such heritage embroidered and appliqued leather tapestry. The aim of the present study is to reveal the type(s) of fibers and dyes used in crafting this rare artwork and the deterioration forms as well. The study used the Stereo Microscope (SM), the Scanning Electron Microscope coupled with EDX unit (SEM-EDX), ATR-FTIR and the MALDI-TOF MS. The object is composed of different layers; a textile substrate, a blank leather layer upon the textile substrate, a blackish leather layer, pre-cut white leather patterns appliqued upon the blackish layer using threads, the entire surface is embroidered with dyed threads. The obtained results revealed that the textile substrate is a plain cotton. The embroidery threads are also cotton, dyed with natural indigo, madder and Persian berries. Dyes' mordants were not verified, probably due to the high proportion of dust and soiling matters. The object is suffering from many deterioration forms; namely drying, brittle fabric and threads, faded dyes, lost threads and parts, stains in both the textile substrate and the embroidery threads, and much amount of dust and soiling matters. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the impact of two isolates of Bacillus simplex (PHYB1 and PHYB9) for controlling the root rot disease of black cumin (Nigella sativa) caused by Fusarium camptoceras (PHYF1) under greenhouse and field conditions at Assiut Governorate, Egypt. The highest reduction percentage of infection (22.5%) was recorded by the treatment of PHYB1 as suspension than the control (60%). Both isolates of Bacillus spp. provided the root and foliar dry weight under greenhouse and seed production in the field. The results showed that the isolate PHYB1 as a formulation gave the highest impact in the root dry weight (0.28 g/plant), followed by PHYB9 (0.22 g/plant), with insignificant difference between them. PHYB9 suspension also provided the highest seed production (27.97 g/plant), whereas PHYB9 as a formulation gave the lowest (24.08 g/plant). Studies on the interaction between Bacillus spp. on F. camptoceras by scanning electron microscope (SEM) revealed that both caused a complete mycoparasitism on the fungal growth. The bacterial growth was seen to adhere and colonize the hyphae, resulting in hyphal tissue maceration. Therefore, the use of both isolates of Bacillus spp. to control root rot disease of black cumin under greenhouse and field conditions can be recommended. [ABSTRACT FROM AUTHOR]

A palynological and multivariate study of six species of Tamarix L. distributed in Egypt was carried out. Pollen morphology was examined by Light Microscope (LM) and Scanning Electron Microscope (SEM). The systematic study of these species was conducted by means of numerical analysis using UPGMA clustering and PCO analysis based on 33 morphological characters, including life form, vegetative parts, fruits, floral characters, seeds and pollen grains. Pollen grains were found to be monads, radially symmetrical, isopolar, small-sized and homocolpate. Tamarix nilotica can be easily separated from other taxa by its subprolate pollen shape. Two major clades were identified by multivariate analysis of morphological characters; one of them included three species, namely T. amplexicaulis, T. passerinoides and T. macrocarpa. Our results indicate that there is a wide range of morphological similarity among the species of section Polyadenia. The other clade included T. aphylla, T. tetragyna and T. nilotica from section Tamarix and section Oligadenia. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the impact of two isolates of Bacillus simplex (PHYB1 and PHYB9) for controlling the root rot disease of black cumin (Nigella sativa) caused by Fusarium camptoceras (PHYF1) under greenhouse and field conditions at Assiut Governorate, Egypt. The highest reduction percentage of infection (22.5%) was recorded by the treatment of PHYB1 as suspension than the control (60%). Both isolates of Bacillus spp. provided the root and foliar dry weight under greenhouse and seed production in the field. The results showed that the isolate PHYB1 as a formulation gave the highest impact in the root dry weight (0.28 g/plant), followed by PHYB9 (0.22 g/plant), with insignificant difference between them. PHYB9 suspension also provided the highest seed production (27.97 g/plant), whereas PHYB9 as a formulation gave the lowest (24.08 g/plant). Studies on the interaction between Bacillus spp. on F. camptoceras by scanning electron microscope (SEM) revealed that both caused a complete mycoparasitism on the fungal growth. The bacterial growth was seen to adhere and colonize the hyphae, resulting in hyphal tissue maceration. Therefore, the use of both isolates of Bacillus spp. to control root rot disease of black cumin under greenhouse and field conditions can be recommended. [ABSTRACT FROM AUTHOR]