1. [Chemical constituents from Clitocybe clavipes and their antitumor activities].
- Author
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Lei QJ, Lyu J, Ma GX, Sun ZC, Xu XD, Wei H, Shi LL, and Lu YM
- Subjects
- Humans, Cell Line, Tumor, Drugs, Chinese Herbal chemistry, Drugs, Chinese Herbal pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Molecular Structure, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic chemistry, Cell Proliferation drug effects
- Abstract
This paper aimed to study the chemical constituents from Clitocybe clavipes. Silica gel, ODS, Sephadex LH-20, and semi-p reparative HPLC were employed to separate the ethanol extract of C. clavipes. Six compounds were identified by ~1H-NMR, ~(13)CNMR,and ESI-MS as clavilactone L(1), clavilactone A(2), clavilactone B(3), clavilactone E(4), clavilactone H(5), and clav ilactone I(6). Among them, compound 1 was a new meroterpenoid with a 10-membered carbocycle connected to a hydroquinone. Theantitumor activities of compounds 1-6 were determined by the methyl thiazolyl tetrazolium(MTT) ass ay. The results showed that compounds 1-6 exerted inhibitory effects on the proliferation of human gastric cancer cells(MGC-803),human non-small cell lung cancer cells(A549), and cervical cancer cells(HeLa). Compound 1 exhibited significant inhibitory activity against MGC-803 cells, with the half maximal inhibitory concentration(IC_(50)) of 11. 76 μmol·L~(-1).
- Published
- 2024
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