1. Combined Flow-Fluorescence in situ hybridization to HHV-8 and EBV reveals the viral heterogeneity of primary effusion lymphoma.
- Author
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Stammler R, Vacher L, Fournier B, Lemaire P, Chauvel C, Silvestrini MA, Knapp S, de Frémont GM, Meignin V, Salmona M, Legoff J, Vanjak A, Dunogué B, Urbain F, Lambotte O, Noël N, Gérard L, Oksenhendler E, Galicier L, Latour S, and Boutboul D
- Subjects
- Humans, Male, Aged, Middle Aged, Female, Herpesviridae Infections virology, Herpesviridae Infections diagnosis, Epstein-Barr Virus Infections virology, Epstein-Barr Virus Infections complications, Aged, 80 and over, Herpesvirus 8, Human genetics, Herpesvirus 8, Human isolation & purification, In Situ Hybridization, Fluorescence methods, Lymphoma, Primary Effusion virology, Herpesvirus 4, Human genetics, Herpesvirus 4, Human isolation & purification
- Abstract
Primary effusion lymphoma (PEL) is a rare B-cell non-Hodgkin lymphoma associated with Kaposi Sarcoma-associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein-Barr virus (EBV) in 60-80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow-Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations., (© 2024 The Author(s). Journal of Medical Virology published by Wiley Periodicals LLC.)
- Published
- 2024
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