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CircBACH1 (hsa_circ_0061395) promotes hepatocellular carcinoma growth by regulating p27 repression via HuR.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2020 Oct; Vol. 235 (10), pp. 6929-6941. Date of Electronic Publication: 2020 Jan 31. - Publication Year :
- 2020
-
Abstract
- In recent years, an increasing number of circular RNAs (circRNAs) have been discovered in hepatocellular carcinoma (HCC). However, the functions of most circRNAs require further investigation. Here, we found that circBACH1 was significantly upregulated in HCC tissues and that high circBACH1 levels were closely associated with poor prognosis. In addition, circBACH1 could promote HCC growth by accelerating cell cycle progression in vitro and in vivo. We next investigated the cellular and molecular mechanisms and discovered that circBACH1 inhibited p27 translation, which influenced cell cycle progression. Moreover, we revealed that circBACH1 could combine directly with HuR using RNA immunoprecipitation assays, pull-down assays, and electrophoretic mobility shift assays. The combination of these molecules facilitated HuR translocation from the nucleus to the cytoplasm according to the fluorescence in situ hybridization and immunofluorescence results. Finally, silencing HuR abrogated circBACH1's inhibition of p27 translation and abolished the circBACH1-induced effect on HCC proliferation. In sum, circBACH1 plays a significant role as an oncogene through the circBACH1/HuR/p27 axis in HCC development.<br /> (© 2020 Wiley Periodicals, Inc.)
- Subjects :
- Animals
Carcinoma, Hepatocellular pathology
Cell Cycle genetics
Cell Line
Cell Line, Tumor
Cytoplasm genetics
Hep G2 Cells
Humans
In Situ Hybridization, Fluorescence methods
Liver Neoplasms pathology
Male
Mice
Mice, Nude
Up-Regulation genetics
Basic-Leucine Zipper Transcription Factors genetics
Carcinoma, Hepatocellular genetics
Cell Proliferation genetics
Cyclin-Dependent Kinase Inhibitor p27 genetics
ELAV-Like Protein 1 genetics
Liver Neoplasms genetics
RNA, Circular genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 235
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 32003018
- Full Text :
- https://doi.org/10.1002/jcp.29589