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The enzyme 2,4′-dihydroxyacetophenone dioxygenase (or DAD) catalyses the conversion of 2,4′-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid with the incorporation of molecular oxygen. Whilst the vast majority of dioxygenases cleave within the aromatic ring of the substrate, DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring. There is evidence that the enzyme is a homotetramer of 20.3 kDa subunits each containing nonhaem iron and its sequence suggests that it belongs to the cupin family of dioxygenases. By the use of limited chymotrypsinolysis, the DAD enzyme from Alcaligenes sp. 4HAP has been crystallized in a form that diffracts synchrotron radiation to a resolution of 2.2 Å. [ABSTRACT FROM AUTHOR]

Taping is often used to manage the high rate of knee injuries in ballet dancers; however, little is known about the effect of taping on lower-limb biomechanics during ballet landings in the turnout position. This study investigated the effects of Kinesiotape ( KT), Mulligan's tape ( MT) and no tape ( NT) on knee and hip kinetics during landing in three turnout positions. The effect of taping on the esthetic execution of ballet jumps was also assessed. Eighteen pain-free 12-15-year-old female ballet dancers performed ballet jumps in three turnout positions, under the three knee taping conditions. A Vicon Motion Analysis system (Vicon Oxford, Oxford, UK) and Advanced Mechanical Technology, Inc. ( Watertown, Massa chusetts, USA) force plate collected lower-limb mechanics. The results demonstrated that MT significantly reduced peak posterior knee shear forces ( P = 0.025) and peak posterior ( P = 0.005), medial ( P = 0.022) and lateral ( P = 0.014) hip shear forces compared with NT when landing in first position. KT had no effect on knee or hip forces. No significant differences existed between taping conditions in all landing positions for the esthetic measures. MT was able to reduce knee and the hip forces without affecting the esthetic performance of ballet jumps, which may have implications for preventing and managing knee injuries in ballet dancers. [ABSTRACT FROM AUTHOR]

The purpose of this study was to compare the effects of Mulligan's tape ( MT) and kinesio tape ( KT) with no tape ( NT) on hip and knee kinematics and kinetics during running. Twenty-nine female recreational runners performed a series of 'run-throughs' along a 10-m runway under the three taping conditions. Two force plates and a 14-camera Vicon motion analysis system ( Oxford Metrics, Inc., Oxford, UK) captured kinematic and kinetic data for each dependent variable from ground contact to toe off. Comparisons of each dependent variable under three taping conditions were assessed through Statistical Package for the Social Sciences ( SPSS; SPSS, Inc., Chicago, Illinois, USA; P-value < 0.01) using repeated measure analyses of variance. For each dependent variable with a P-value < 0.01, repeated measures with pairwise comparisons and Bonferroni adjustment were conducted to compare the three taping conditions. MT induced a significant reduction in anterior and posterior hip forces, knee flexion angular velocity, knee extensor moments, and hip flexion and extension moments compared with NT and KT ( P = 0.001). There was no difference in hip or knee, kinematics or kinetics, between KT and NT ( P = 1.000). MT appears to influence hip and knee biomechanics during running in an asymptomatic sample, whereas KT appeared to be biomechanically not different from NT. [ABSTRACT FROM AUTHOR]

The enzyme 2,4′-dihydroxyacetophenone dioxygenase (DAD) catalyses the conversion of 2,4′-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid with the incorporation of molecular oxygen. Whilst the vast majority of dioxygenases cleave within the aromatic ring of the substrate, DAD is very unusual in that it is involved in C-C bond cleavage in a substituent of the aromatic ring. There is evidence that the enzyme is a homotetramer of 20.3 kDa subunits, each containing nonhaem iron, and its sequence suggests that it belongs to the cupin family of dioxygenases. In this paper, the first X-ray structure of a DAD enzyme from the Gram-negative bacterium Alcaligenes sp. 4HAP is reported, at a resolution of 2.2 Å. The structure establishes that the enzyme adopts a cupin fold, forming dimers with a pronounced hydrophobic interface between the monomers. The catalytic iron is coordinated by three histidine residues (76, 78 and 114) within a buried active-site cavity. The iron also appears to be tightly coordinated by an additional ligand which was putatively assigned as a carbonate dianion since this fits the electron density optimally, although it might also be the product formate. The modelled carbonate is located in a position which is highly likely to be occupied by the α-hydroxyketone group of the bound substrate during catalysis. Modelling of a substrate molecule in this position indicates that it will interact with many conserved amino acids in the predominantly hydrophobic active-site pocket where it undergoes peroxide radical-mediated heterolysis. [ABSTRACT FROM AUTHOR]

The article presents a case study of a 21-year-old male with severe haemophilia A and a FVIII inhibitor. It describes the bilateral upper limb compartment syndrome induced by strenuous exercise in a patient with haemophilia A and a low titre inhibitor. The case highlights the importance of clinical intervention for patients with haemophilia who develop significant musculoskeletal complications and identifies the risk of non-traumatic compartment syndrome in persons with haemophilia.

Butterflies are among the most effective bioindicators of climate change; however, their diversity in many rural areas of the Central Himalayas remains understudied. This study provides an assessment of butterfly diversity in the foothills of Bhorletar, Madhya Nepal Municipality, Lamjung District, Nepal, within an elevation range of 420–600 m. Conducted between July 2019 and January 2021, the survey involved opportunistic observations and photography of adult butterflies in their natural habitats, with sampling occurring six times each month. The study aimed to investigate the species composition, richness, and abundance of butterflies across the survey period and identify seasonal changes in species composition and richness. A total of 94,009 individuals across 226 species, 129 genera, and six families were documented. During this study, Halpe arcuata Evans, 1937 and Hasora taminatus bhavara Fruhstorfer, 1911 were recorded for the first time in Nepal. Additionally, Halpe filda Evans, 1949 and Ctenoptilum vasava vasava (Moore, [1866]) were recorded for only the second and third times, respectively, in Nepal, following a gap of approximately three decades. The most abundant species was Pieris canidia indica Evans, 1926 (Relative Abundance [RA] 2.55%), followed by Pseudozizeeria maha maha (Kollar, [1844]) (RA 2.13%). Species richness showed an annual bimodal distribution, peaking in April (180 species) and August (161 species), while the lowest richness was observed in January and February, with 68 and 75 species, respectively. Diversity indices included a Shannon–Wiener index of 4.71, Pielou's J index of 0.87, an effective number of species of 111.24, and Margalef's richness index of 19.65, indicating high species diversity with a well‐balanced mix of species evenness and richness. This study offers the first peer‐reviewed checklist of butterflies from Bhorletar, providing crucial baseline data for future research and conservation efforts, and highlights the remarkable seasonal and species diversity within the region. [ABSTRACT FROM AUTHOR]

Aim: To the determinants and the underlying mechanism of health literacy, social support, and resilience on the health‐related quality of life (HRQoL) among older stroke survivors. Design: A cross‐sectional design was applied at four comprehensive hospitals in Chongqing via convenience sampling from January 2020 to June 2021. Methods: Health literacy, social support, and resilience were designed as independent variables, and HRQoL was measured as a dependent variable. Structural equation modelling with the bootstrap method was used to test the hypotheses. Results: The theoretically derived model exhibited a good fit (χ2/df ratio = 2.830, GFI = 0.987, CFI = 0.978, RMSEA = 0.066). Health literacy (β = 0.12, p < 0.05) and social support (β = 0.14, p < 0.05) directly affect HRQoL. Resilience (β = 0.40, p < 0.01) also mediated the relationship between health literacy, social support, and HRQoL. The three variables explaining 29.0% of HRQoL variance. Patient or Public Contribution: There was no direct patient or public involvement in the design, conduct, or reporting of this study. Participants were recruited through convenience sampling from four comprehensive hospitals in Chongqing, and their perspectives or contributions were not explicitly sought. The study focused on examining the determinants and underlying mechanism of health literacy, social support, and resilience on the health‐related quality of life among older stroke survivors. Nonetheless, the findings of this research may inform the development of interventions aimed at improving the health‐related quality of life in post‐stroke older patients. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study was to determine the reproductive cycle and spawning frequency of the sea cucumber Holothuria tubulosa in the northeastern Aegean Sea. Methods: The study covered a period of 12 months, beginning in October 2018 and concluding in September 2019. During this period, sea cucumber samples were collected monthly. Macroscopic and histological examinations of gonadal tubules were conducted to provide credible information for fishery management and aquaculture. Result: The sex ratio of the sampled population was 1:1.3, with 95 males and 130 females. The drained body weights at 50% maturity were 70.90 g for females and 70.80 g for males. The total length at 50% maturity was estimated at 18.58 cm for females and 18.05 cm for males. Gonad maturation was initiated in March and progressed to spawning stages between July and August. From September to February, the gonads were in postspawning and recovery stages. A strong linear correlation was determined between the gonad index and the seawater temperature in the area. Conclusion: The information provided is expected to improve stock management, enforce fishery regulations, and support controlled breeding programs. These measures aim to preserve the natural populations of Holothuria tubulosa and guarantee the long‐term viability of the species in the wild. Impact statementThe Holothuria tubulosa is the most abundant commercial sea cucumber species found in the Mediterranean Sea, particularly in the Aegean Sea. This species plays an important ecological role in nutrient regeneration and surface sediment mineralization in the benthic zone; however, there is significant catch pressure on natural stocks. This study revealed the reproductive cycle of this Mediterranean Sea cucumber species, offering crucial data for sustainable fisheries and aquaculture. By understanding their breeding patterns, we can better manage wild populations and develop controlled breeding programs, ensuring the long‐term survival of the species and supporting healthy marine ecosystems. [ABSTRACT FROM AUTHOR]

Key Clinical Message: The use of DN to the muscular trigger points and distal periosteal enthesis of the levator scapulae may be a useful adjunct intervention within a multi‐modal plan of care for the management of work‐related chronic tension‐type headaches associated with LSS. Chronic tension‐type headaches (CTTH) have a lifetime prevalence of 42% and account for more lost workdays than migraine headaches. Dry needling (DN) is being increasingly used by physical therapists in the management of CTTH; however, to date, the supporting evidence is limited. The purpose of this case report was to describe how three sessions of DN targeting myofascial trigger points in the levator scapulae (LS) muscle and its distal enthesis was used to treat a 63‐year‐old male patient who presented with work‐related CTTH associated with levator scapulae syndrome (LSS). The patient was treated for five visits over the course of 2 months. At discharge and 6‐month follow‐up, the patient reported full resolution of symptoms. Self‐report outcomes included the numeric pain rating scale and the Neck Disability Index. The use of DN to the LS muscle and its distal enthesis may be a valuable addition to a multi‐modal plan of care in the treatment of work‐related CTTH associated with LSS. [ABSTRACT FROM AUTHOR]

The authors review the article "Prescription Drug Use and Abuse: Risk Factors, Red Flags, and Prevention Strategies" (J. H. Isaacson, J. A. Hopper, D. P. Alford, & T. Parran, 2005), which provides an overview of the recent increase in prescription opioid abuse and dependence from the physician's perspective. In the present article, the authors discuss the counselor's role in identifying issues related to client misuse and abuse of prescription opioids. Associated terminology, common drug acquisition strategies, and signs of problematic use are provided and discussed. [ABSTRACT FROM AUTHOR]

Introduction: Weight loss is hard to achieve and even harder to maintain. Engaging in effortful behavioural change to manage body weight can sometimes result in feelings of guilt and shame. Self‐compassion, the tendency to find kindness for oneself in times of struggle, may facilitate coping with the unique challenges of weight management. This study assessed whether a remotely delivered self‐compassion intervention improved weight management outcomes when delivered as a supplement to an existing digital behavioural weight management programme, Weight Watchers (WW). Method: Using a mixed‐method study design, 249 adults seeking to manage weight were randomized to either the WW programme or WW supplemented with the self‐compassion for weight management intervention (SC4WM). Participants completed measures of self‐compassion, eating behaviour, physical activity, body weight and emotional well‐being along with potential moderators, including weight self‐stigma, eating restraint, psychological coping and perceived stress at baseline, post‐intervention (4 weeks) and follow‐up (12 weeks). Results: There was no evidence that the SC4WM intervention had a significantly different effect than WW alone. Other than body weight, all outcomes improved over time in both groups. Self‐compassion was slightly higher overall in the SC4WM group (p =.05), with this group reporting higher self‐kindness at 4 weeks (p =.014) and lower self‐judgement at 12 weeks (p =.023) compared to the control group. Conclusions: Although the SC4WM intervention group did show a small increase in self‐kindness and reduction in self‐judgement, weight management outcomes were not improved over and above the existing WW programme. Recommendations for adapting the SC4WM intervention to improve efficacy to augment weight management outcomes are provided. [ABSTRACT FROM AUTHOR]

Limpets play an important role in structuring the intertidal fringe, one of the most heavily impacted marine habitats. A large number of limpet species are listed as Endangered or Vulnerable, including Patella ferruginea, one of the most endangered marine invertebrates in the Mediterranean Sea.Relocation attempts have been made as a conservation tool for reintroductions and population reinforcements for P. ferruginea. Some relocations were mandatory because of port infrastructure repairs in areas where viable populations of this limpet occurred. However, most relocations attempted to date have experienced high mortality rates, leading to the Spanish National Conservation Strategy not recommending this approach for this species.In this study, 84 individuals of P. ferruginea were relocated, using artificial portable plates (APPs) installed on the ripraps of the Port of Ceuta (Western Mediterranean Sea). These plates were divided into two groups and relocated: (i) as a translocation to a nearby site with similar environmental conditions to the donor area (procedural control); and (ii) as a reinforcement translocation to a relatively distant location (30 km, Algeciras Bay) (experimental procedure). The mortality of the translocated individuals in the procedural control after 10 weeks was similar (6.6%) to that expected for natural populations, whereas the mortality rate for individuals in the reinforcement translocation was higher (26.6%).The APPs have been proven to be a feasible method for translocation, as they result in relatively low mortality by preventing the stress and damage caused by limpet detachment from substrata. The constraints caused by reinforcement translocation to a new environment seem to be the cause of the increased mortality, as the translocated limpets changed their home scar at a much higher rate than the control limpets did. [ABSTRACT FROM AUTHOR]

Introduction: Hamstring strain injury (HSI) remains a performance, economic, and player availability burden in sport. High‐speed running (HSR) is cited as a common mechanism for HSI. While evidence exists regarding the high physical demands on the hamstring muscles in HSR, meta‐analytical synthesis of related activation and kinetic variables is lacking. Methods: A systematic search of Medline, Embase, Scopus, CINAHL, SportDiscus, and Cochrane library databases was conducted in accordance with the PRISMA 2020 guidelines. Studies reporting hamstring activation (electromyographic [EMG]) or hamstring muscle/related joint kinetics were included where healthy adult participants ran at or beyond 60% of maximum speed (activation studies) or 4 m per second (m/s) (kinetic studies). Results: A total of 96 studies met the inclusion criteria. Run intensities were categorized as "slow," "moderate," or "fast" in both activation and kinetic based studies with appropriate relative, and raw measures, respectively. Meta‐analysis revealed pooled mean lateral hamstring muscle activation levels of 108.1% (95% CI: 84.4%–131.7%) of maximal voluntary isometric contraction (MVIC) during "fast" running. Meta‐analysis found swing phase peak knee flexion internal moment and power at 2.2 Newton meters/kilogram (Nm/kg) (95% CI: 1.9–2.5) and 40.3 Watts/kilogram (W/kg) (95% CI: 31.4–49.2), respectively. Hip extension peak moment and power was estimated as 4.8 Nm/kg (95% CI: 3.9–5.7) and 33.1 W/kg (95% CI: 17.4–48.9), respectively. Conclusions: As run intensity/speed increases, so do the activation and kinetic demands on the hamstrings. The presented data will enable clinicians to incorporate more objective measures into the design of injury prevention and return‐to‐play decision‐making strategies. [ABSTRACT FROM AUTHOR]

Objectives: The digit ratio (2D:4D) is a possible marker of prenatal hormone exposure. The purpose of this study was to explore the relationships between digit ratio (2D:4D) and anthropometric indexes in Chinese children and adolescents., Methods: This study is a cross-sectional study. A school-based survey among 685 children and adolescents aged 8-15 years were conducted by stratified cluster sampling. The length of index finger (2D) and ring finger (4D) of the left hand, height, sitting height (ST), weight, chest circumference (CC), waist circumference (WC), hip circumference (HC), and abdominal skinfold thickness (AST) were measured. Pearson correlation and multivariate linear regression were used to analyze associations between 2D:4D and above indexes., Results: In girls, 2D:4D was positively related to WC, AST, waist-to-height (WHtR), waist-to-hip ratio (WHR) after adjusting for ages (p < 0.05). The WC, AST, WHtR, and WHR among girls with 2D:4D ≥ 1 were significantly higher than those among girls with 2D:4D < 1, respectively (p < 0.05). However, there was no correlations between digit ratio (2D:4D) and above anthropometric indexes in boys (p > 0.05)., Conclusions: The 2D:4D was related to anthropometric indexes in girls, which suggests that the maternal prenatal hormone exposure might be related to the anthropometric indexes of their female offspring., (© 2024 Wiley Periodicals LLC.)

Aim: To determine whether the method of telehealth delivery (audioconferencing or videoconferencing) affects the physiotherapy management of adults with inherited bleeding disorders. Methods: A cross‐sectional observational study was utilised involving 40 physiotherapy consultations (23 initial consultations and 17 follow‐up consultations) of adults (>18) with a diagnosed bleeding disorder. Each consultation involved an initial audioconferencing component followed immediately by a separate videoconferencing component. Following each component, the physiotherapist utilised the clinical information gathered to formulate and record a management plan, and additionally recorded their confidence in this plan. Differences between the management plans and clinician confidence were recorded, including where applicable the visual information prompting a change in management plans. Results: Audioconferencing and videoconferencing management plans differed in 40% of all consultations, including 52.0% of initial consultations and 23.5% of follow‐up consultations. Among consultations where management plans differed, this was prompted by visual information related to the anatomic location of symptoms (31.3%), the absence/presence of swelling (31.3%), joint range of movement (25.0%), and general appearance (12.5%). Median self‐reported clinician confidence of management plans increased significantly from 70.0% following audioconferencing to 93.0% following videoconferencing. Conclusion: When utilizing telehealth, the choice between audioconferencing or videoconferencing may affect physiotherapy management of adults with bleeding disorders, particularly with initial consultations. Videoconferencing potentially leads to more appropriate management plans, clinician confidence and utilization of healthcare resources. Further high‐quality studies are required to confirm the findings of this study. [ABSTRACT FROM AUTHOR]

Drought tolerance varies greatly across Vitis vinifera cultivars, depending on physiological responses and structural and morphological adaptations. In this study, responses to water stress were examined in three extensively cultivated varieties from Northern Italy. Over the course of two seasons, mature potted vines were subjected to a 12 or 13‐day period of water restriction. Vine water relations were investigated using measures of water potential, gas exchanges, and leaf ABA content. Leaf angle response to increasing water stress was analysed in the four cultivars as a mechanism that improves stress tolerance. Different physiological responses were observed among cultivars, suggesting a near‐isohydric water‐use strategy for Moscato and a near‐anisohydric one for Garganega, Glera and Merlot. Results of leaf ABA analysis highlighted a variability among the studied varieties, indicating higher contents and lower sensitivity to ABA for the anisohydric ones. In all varieties, a similar increase in midday leaf inclination was observed in response to decreasing stem water potentials, indicating that leaf angle adjustments may represent a common adaptive response to drought. These findings increase the understanding of the leaf physiological and structural mechanisms that contribute to water stress tolerance in grapevine, supporting a more efficient cultivar selection to cope with the expected changes in Mediterranean climate. [ABSTRACT FROM AUTHOR]

Diamond based quantum sensing for on‐chip electromagnetic field imaging is an emerging field. While smaller diamond probes offer higher spatial resolution, their lower fluorescence intensity poses a challenge for the traditional Avalanche Photo Diode. In this work, we propose a tapered fiber diamond probe containing Nitrogen‐vacancy center and utilize a single photon counter module as the detector. A time domain differential pulse sequence is introduced to suppress noise caused by laser intensity fluctuation. By analyzing the relationship between the signal‐to‐noise ratio (SNR) and parameters of differential pulse sequence, we derived the formula of SNR and the basic cycle (M $M$), and obtained the optimal SNR in the same measurement time through trade‐off between two different kinds of noise. The technique was applied to a waveguide microstrip filter chip, accurately imaging the current density distribution. Our results have potential applications in on‐chip testing and failure analysis. [ABSTRACT FROM AUTHOR]

Purpose: Tibiofemoral Varus Malalignment (TFRV) contributes to overuse injuries by altering lower limb biomechanics. Both Posterior X Taping (PXT) and Real Time Feedback (RTF), have each been recommended for subjects with TFRV as they are thought to enhance control of excessive tibiofemoral rotations. This paper evaluates this claim. Methods: A total of recreational male 24 athletes with TFRV participated in the current study. Kinematic and electromyography variables of lower extremity were synchronously ​recorded on five consecutive repetitions of the single-legged-squat (SLS) and forward-step-down) FSD) tasks before and after applications of PXT and RTF. Results: The subjects at post-intervention in RTF group exhibited decreased hip adduction during FSD, and decreased hip adduction and internal rotation during eccentric and concentric phases of the SLS; Additionally, we observed increased gluteus medius activity during eccentric phase of the SLS and FSD tasks. In contrast, subjects at the post-intervention in PXT group exhibited decreased tibiofemoral external rotation and increased ankle external rotation during all the phases of both SLS and FSD tasks. Conclusion: These results suggest that the PXT and RTF interventions are recommended to immediately improve the functional defects of the subjects with TFRV during SLS and FSD tasks. [ABSTRACT FROM AUTHOR]

Augmented feedback (aF) positively influences motor performance by enhancing motivation and/or by providing information about task execution. It was speculated that aF-induced performance increments that rely on motivation should also occur when providing incorrect aF, while performance increments that rely on guidance towards "successful executions" (i.e. improved performances) should only occur when aF is correct. We further hypothesised that the informational content of aF is more important in more complex motor tasks. Thus, 32 participants received two forms of aF (correct, incorrect) during maximal voluntary contractions (MVC's; maximise force without time constraints; less complex) and maximal explosive contractions (MEC's; maximise force in the shortest possible way; more complex) of the knee extensors. Peak torque (MVC), peak rate of torque development (MEC) and EMG signals of rectus femoris (RF) and vastus lateralis were recorded. Correct and incorrect aF significantly enhanced MVC performance, indicating that performance improvements resulted mainly from the motivational property of aF. The observed trend towards increased RF muscle activity supports this conclusion. In contrast, while correct aF positively impacted MEC performance, incorrect aF had a negative influence. This indicates that the informational property of aF guided participants towards movement executions resulting in improved (correct aF) or decreased (incorrect aF) performances. The observed simultaneous decrease in muscle activity suggests that participants changed motor strategy, supporting the guiding role of aF. Our results demonstrate that the motivational aspect of aF dominates in maximal tasks with lower complexity (MVC), while the informational aspect is used during more complex maximal tasks (MEC). Augmented feedback (aF) can influence performance by enhancing the motivation and/or by providing information about the execution of a task. Our results demonstrate that over the short-term, the motivational aspect of aF dominates in maximal tasks with lower complexity (maximal voluntary contractions). In contrast, the informational aspect will predominantly be used during more complex maximal tasks (maximal explosive contractions). This is the first study distinguishing between the motivational and informational aspects of aF during maximal motor tasks. Future research should focus on the long-term effects of these two separate aspects of aF. [ABSTRACT FROM AUTHOR]

Objective: To assess the association between hypermobility spectrum disorders/hypermobile type Ehlers Danlos Syndrome (HSD/hEDS) and migraine in a national sample of adolescents in Israel. Background: The association between HSD/hEDS and migraine is unclear, even more so in pediatric populations. Methods: This population‐based, cross‐sectional study included 1,627,345 Israeli adolescents (945,519/1,626,407 [58%] males; mean age 17 ± 0.5 years) who were medically assessed before mandatory military service during 1998–2020. Diagnoses of migraine with at least one attack per month (active migraine) and HSD/hEDS were confirmed by certified specialists. The prevalences of active migraine in adolescents with and without HSD/hEDS were computed and the association between HSD/hEDS and active migraine was examined. Results: Active migraine was significantly more prevalent in adolescents with HSD/hEDS (307/4686 [6.5%]) compared to those without HSD/hEDS (51,931/1,621,721 [3.2%]) (OR = 2.16, 95% CI 1.90–2.45). The association between HSD/hEDS and active migraine persisted in a multivariable analysis (OR = 2.08, 95% CI 1.85–2.34) and in several sensitivity analyses. Conclusions: We found a significant association between HSD/hEDS and active migraine in both male and female adolescents. Clinical awareness of the association can promote early diagnosis and treatment of migraine. Further research is required to identify appropriate pharmacologic and nonpharmacologic migraine treatment strategies for individuals with HSD/hEDS. [ABSTRACT FROM AUTHOR]

Phototherapy incorporating photobiomodulation therapy and antimicrobial photodynamic therapy has been utilised as antioxidants in symptomatic oral lichen planus (OLP) management; however, its role of intervention remains controversial. The aim of this systematic review of CRD42021227788 PROSPERO (an international prospective register of systematic reviews in health and social care) registration number was to oversee and determine phototherapy efficacy in patients with symptomatic OLP, identifying and bridging the literature gaps by proposing recommendations for future studies. A search strategy was developed in consistent with Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines. Various electronic databases were exercised to search for randomised controlled clinical trials (RCTs). Several search engines were employed to analyse a total of 177 studies of which nine included. A wide range of utilised laser and light‐emitted diode wavelengths between 630 and 808 nm and irradiance ranged between 10 and 13 mW/cm2 were noted. 67% of studies reported a high risk of bias and a high heterogeneity obtained from numerical data for quantitative analysis, therefore meta‐analysis was impossible to conduct. Despite inconsistency and diversity in phototherapy parameters, treatment protocols, photosensitiser (type, concentration and method of application) and outcome assessment tools, the majority of the studies showed positive results compared with standard care treatments. Hence, a necessity to perform well‐designed RCTs with robust methodology is warranted, after acknowledging the current drawbacks and addressing the suggested recommendations highlighted in our review. Moreover, advanced knowledge in understanding further phototherapy‐antioxidants molecular mechanistic in symptomatic OLP is required. [ABSTRACT FROM AUTHOR]

Background: To explore attitudes to telehealth education and experiences incorporating telehealth education into entry‐to‐practice physiotherapy programs in Australia, from the perspective of university educators. Methods: Qualitative design based on a constructivist paradigm and a phenomenological approach. Sixteen university educators (who had a responsibility for telehealth curriculum or oversight of the broader curriculum in an entry‐to‐practice physiotherapy programme at an Australian university) were recruited. Individual semi‐structured interviews were conducted via Zoom. Interviews were recorded, transcribed verbatim, and analysed thematically using an inductive approach. Results: Three themes (with associated subthemes) were identified: (i) telehealth education has a role in contemporary physiotherapy practice (COVID‐19 pandemic was a driver for telehealth education, acknowledgement that telehealth is here to stay and identified areas of focus for telehealth education and training); (ii) telehealth education and training vary substantially (content delivered and assessment of telehealth competency is ad hoc and student exposure to telehealth on clinical placements is inconsistent); (iii) challenges in telehealth education (finding space and time in the curriculum, as well as insufficient knowledge and expertise of staff, are challenges for implementation of telehealth education, however, course and subject development and/or reviews provide opportunities for implementing telehealth education and training). Conclusion: Current content and volume of telehealth education and training in entry‐to‐practice physiotherapy programs across Australia varies substantially. Although educators believe telehealth is an important component of contemporary physiotherapy practice, many barriers exist for including telehealth training into the curriculum. [ABSTRACT FROM AUTHOR]

Upper neck impairments are more prevalent in patients with temporomandibular disorders (TMDs) but the differences between specific types of TMDs are unclear. This study evaluated the distribution of such impairments among different forms of TMD. In total, 116 participants (86 women and 30 men, age range 21-75 yr) were investigated. Forty-two individuals had no TMDs and were assigned to the control group. The remaining 74 patients were assigned to one of three groups based on the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) findings: pain-related (n = 37); intra-articular (n = 17); or mixed (combined pain-related and intra-articular) (n = 20). Analyses of impairments included between-group comparisons of key parameters of upper neck performance (active/passive mobility and muscular capabilities) and pain (subjective neck disability and pain sensitivity). Patients in the pain-related and mixed TMD groups were found to have decreased upper neck mobility in the cervical flexion-rotation test compared with patients in intra-articular and control groups, as well as poorer capabilities of the deep neck flexor muscles in the cranio-cervical flexion test compared to the control group. It was concluded that patients with pain-related TMD diagnoses are more likely to experience significant upper-neck hypomobility and poor muscular capabilities than patients with intra-articular diagnoses of TMD., (© 2020 Eur J Oral Sci.)

Membrane protein purification by means of detergents is key to isolating membrane‐bound therapeutic targets. The role of the detergent structure in this process, however, is not well understood. Detergents are optimized empirically, leading to failed preparations, and thereby raising costs. Here we evaluate the utility of the hydrophilic‐lipophilic balance (HLB) concept, which was introduced by Griffin in 1949, for guiding the optimization of the hydrophobic tail in first‐generation, dendritic oligoglycerol detergents ([G1] OGDs). Our findings deliver qualitative HLB guidelines for rationalizing the optimization of detergents. Moreover, [G1] OGDs exhibit strongly delipidating properties, regardless of the structure of the hydrophobic tail, which delivers a methodological enabling step for investigating binding strengths of endogenous lipids and their role for membrane protein oligomerization. Our findings will facilitate the analysis of challenging drug targets in the future. [ABSTRACT FROM AUTHOR]

Breast cancer is still the leading cause of women's death due to relapse and metastasis. In vitro tumor models are considered reliable tools for drug screening and understanding cancer‐driving mechanisms due to the possibility of mimicking tumor heterogeneity. Herein, a 3D breast cancer model (3D‐BCM) is developed based on enzymatically‐crosslinked silk fibroin (eSF) hydrogels. Human MCF7 breast cancer cells are encapsulated into eSF hydrogels, with and without human mammary fibroblasts. The spontaneously occurring conformational change from random coil to β‐sheet is correlated with increased eSF hydrogels' stiffness over time. Moreover, mechanical properties analysis confirms that the cells can modify the stiffness of the hydrogels, mimicking the microenvironment stiffening occurring in vivo. Fibroblasts support cancer cells growth and assembly in the eSF hydrogels up to 14 days of culture. Co‐cultured 3D‐BCM exhibits an upregulated expression of genes related to extracellular matrix remodeling and fibroblast activation. The 3D‐BCM is subjected to doxorubicin and paclitaxel treatments, showing differential drug response. Overall, these results suggest that the co‐culture of breast cancer cells and fibroblasts in eSF hydrogels allow the development of a mimetic in vitro platform to study cancer progression. This opens up new research avenues to investigate novel molecular targets for anti‐cancer therapy. [ABSTRACT FROM AUTHOR]

Background: Pericardial disease accounts for approximately 10 per cent of all cardiac referrals. It is seen in all species, but is most commonly diagnosed in dogs and occasionally in cats. Pericardial disease comprises both acquired and congenital conditions, of which pericardial effusion (PE) associated with acquired disease is most common. PEs are most frequently either idiopathic or secondary to neoplasia, although other causes such as left atrial tear (secondary to myxomatous valvular disease), infectious pericarditis, an extension of congestive heart failure (CHF) and secondary to trauma are all possible causes. Congenital pericardial disease is rare, but includes peritoneopericardial diaphragmatic hernia, benign intrapericardial cysts and pericardial defects. This rare and small group of congenital conditions are important, as prompt recognition and management usually infers an excellent prognosis. Where there is sufficient volume within the pericardial sac, intrapericardial pressure can rise, leading to cardiac tamponade and signs of right‐sided CHF (R‐CHF) and low cardiac output. Importantly, management of R‐CHF in cases of pericardial disease requires a completely different approach to other diseases causing R‐CHF. Aim of the article: This article reviews the most common conditions that cause PE, common clinical signs and appropriate management strategies. [ABSTRACT FROM AUTHOR]

Research question/issue: Innovation has been a constant feature of the tales of transition and transformation. State ownership as the engine of innovation and technological change may be juxtaposed with the "liability of stateness" and the notion that "privatization works." This study seeks to investigate the relationship between legal ownership and innovation inputs and outputs, while accounting for the moderating effect of institutional quality on this relationship. Research findings/insights: We exploit unique data from a very large‐scale panel survey of enterprises (65,750 firms between 2006 and 2014) in Vietnam, a fast‐growing but understudied transition country, and apply advanced methodologies that control for the endogeneity of institutions. Our findings point to the continued dominance of state‐owned enterprises (SOEs) in innovation activities in Vietnam. However, the returns to innovation in SOEs accrue only up to a point and improving institutional quality serves to diminish their advantage over privately‐owned enterprises (POEs) and to level the playing field. Theoretical/academic implications: We employ an integrated framework that develops predictions from resource dependence, agency, and institutional theories to explore the direct and contingent influences of ownership and institutional quality on firm‐level innovation activities. Our study contributes to the growing literature on state ownership and innovation in transition and emerging economies and the recent calls for greater attention to local institutional context and revising the existing theories on "state underperformance." Practitioner/policy implications: This study offers insights to policy makers in enhancing the quality of local institutions. Higher‐quality institutions moderate the advantages state ownership confers and ameliorate the disadvantages associated with private ownership. [ABSTRACT FROM AUTHOR]

Pseudomonas aeruginosa (PA) is an opportunistic human pathogen, which is involved in a wide range of dangerous infections. It develops alarming resistances toward antibiotic treatment. Therefore, alternative strategies, which suppress pathogenicity or synergize with antibiotic treatments are in great need to combat these infections more effectively. One promising approach is to disarm the bacteria by interfering with their quorum sensing (QS) system, which regulates the release of various virulence factors as well as biofilm formation. Herein, this work reports the rational design, optimization, and in‐depth profiling of a new class of Pseudomonas quinolone signaling receptor (PqsR) inverse agonists. The resulting frontrunner compound features a pyrimidine‐based scaffold, high in vitro and in vivo efficacy, favorable pharmacokinetics as well as clean safety pharmacology characteristics, which provide the basis for potential pulmonary as well as systemic routes of administration. An X‐ray crystal structure in complex with PqsR facilitated further structure‐guided lead optimization. The compound demonstrates potent pyocyanin suppression, synergizes with aminoglycoside antibiotic tobramycin against PA biofilms, and is active against a panel of clinical isolates from bronchiectasis patients. Importantly, this in vitro effect translated into in vivo efficacy in a neutropenic thigh infection model in mice providing a proof‐of‐principle for adjunctive treatment scenarios. [ABSTRACT FROM AUTHOR]

Pregnant and parenting women living with HIV (WLWH) face high levels of psychological stress and mental illness but lack tailored and acceptable psychosocial treatments. The research team sought to inform the adaptation of a mindfulness intervention for pregnant and parenting WLWH through focus groups exploring psychosocial treatment needs and mindfulness intervention preferences. The research team conducted focus groups with pregnant and parenting WLWH (n = 16) and case managers (n = 6) recruited from a community‐based enhanced case management program. The research team utilised an iterative inductive approach to coding of the transcripts from these focus groups. Five themes emerged: stressors, signs of stress, coping, lack of access and acceptability of care, and motivation and trust in care engagement. These focus groups revealed a desire for a group intervention that could decrease isolation while protecting against involuntary disclosure of HIV status. Participants expressed openness to mindfulness skills for coping with stress. The focus group participants' preference for a non‐stigmatising group intervention supports the potential of a mindfulness‐based group intervention to reduce stress and improve the mental health of pregnant and parenting women living with HIV. [ABSTRACT FROM AUTHOR]

Objectives: Individuals who have a parent with mental illness are more likely to experience mental illness than their contemporaries. As such, it is valuable to examine potential psychological resources, which might assist these individuals to experience good mental health throughout their lifespan. We aimed to learn how clinicians perceive self‐compassion, and how it can be incorporated into therapy with clients who have parents with mental illness. Design: A qualitative interview design was employed to explore clinicians' perspectives and experiences. Methods: Eight mental health clinicians experienced in working with clients who have parents with mental illness were interviewed. Interpretative phenomenological analysis was used to establish themes representing the clinicians' perspectives and experiences of incorporating self‐compassion into their work. Results: This study found that clinicians were generally positive about incorporating self‐compassion into interventions with clients who are children of parents with mental illness. The participants noted barriers to self‐compassion for these clients, namely a poor sense of self and divided loyalty between self and family. Participants recommended taking time and care, building rapport and involving others when cultivating self‐compassion with those who have parents with mental illness. Conclusions: This group of clinicians viewed self‐compassion as relevant to clients whose parents have mental illness and believe it can be introduced therapeutically in various ways. Suggestions are made for tailoring self‐compassion training to the needs and experiences of this group. [ABSTRACT FROM AUTHOR]

Sub‐zero temperature sensors (SZTSs) have potential applications in safely storing COVID‐19 vaccines. Herein, an SZTS based on laser‐induced carbonization (LIC) achieved by a nanosecond infrared laser with a wavelength of 1064 nm is reported. Direct laser writing is adopted for laser‐induced carbon in Kapton polyimide sheets with a thickness of 125 µm. The sensor exhibits a good linear change in resistance to sub‐zero temperatures ranging from 0 to −150 °C, where the coefficient of determination adjusted R‐square (R2) value is 0.99238, which indicates a good linear fit. The sensor exhibits a stable static response at all temperatures over time. The dynamic responses by controlling the liquid nitrogen gas and placing an ice cube on the sensor are also measured to validate the sensor. Notably, the electrical performance of the sensor remains stable even after 15 h. The sensor response of the LIC sample validates the 3D variable range‐hopping charge transport mechanism, governed by the Mott equation with a good linear fit, which is mainly owing to disorder in its structure. LIC‐based SZTSs can enable sensors that are ultra‐fast to fabricate, roll‐to‐roll processable, economical, and more significantly, can be interfaced with flexible printed circuit boards without any additional interfacing. [ABSTRACT FROM AUTHOR]

Background: Exergaming, one of the most recognized virtual rehabilitation tools, has been shown to be useful for promoting physical activity and enhancing postural stability for neurologic conditions. However, studies with exergaming programs for patients with knee osteoarthritis (OA) are limited. Aims: We aimed to investigate the effects of exergaming given by visual and auditory stimulated assisted joint training device in addition to the conventional physiotherapy program on pain intensity, range of motion (ROM), functional status, kinesiophobia, proprioceptive acuity, muscle strength, and postural stability in patients with knee OA. Study Design: Randomized controlled clinical trial. Methods: Sixty patients (47 female, 13 male) with knee osteoarhritis aged 40–65 years (57.36 ± 7.26) who were at stage of two to three according to the Kellegren Lawrence radiological evaluation were included in the study. The patients were randomly divided into two groups as study group (conventional physiotherapy + exergaming) and control group (conventional physiotherapy). Electrotherapy and exercise program were applied to both of the groups along 5 days a week for 6 weeks but exergaming only applied to study group. ROM, pain intensity, proprioceptive acuity, kinesiophobia, muscle strength, and postural stability of the patients were evaluated at the beginning and end of the treatment. Results: In the intra‐group analyses of all the assessments of the patients, there was a significant difference in the positive direction in both groups, except for the postural stability values. In the intra‐group analyses of postural stability, there was a significant increase only in the study group. In comparison between the groups, proprioceptive acuity, ROM, functional status, and postural stability scores were significantly increased in the study group according to the control group; pain and kinesiophobia decreased significantly. Conclusion: In this study, the exergaming accompanied with conventional physiotherapy programs resulted more positive improvements on pain, ROM, postural stability, kinesiophobia, proprioceptive acuity, and functional status in patients with knee OA compared to the conventional physiotherapy program alone. [ABSTRACT FROM AUTHOR]

Based on a case study of a large Swedish local government municipality, we explored the extent to which a cloud‐based enterprise resource planning (CERP) system enabled the role performance of public sector management accountants. Our findings suggest that the CERP system enabled central management accountants to mobilize their specific expertise because it eliminated manual work, increased transparency, and made them feel more comfortable with the numbers. However, the less flexible features of a CERP system provided by external vendors, such as limited customization, posed a challenge for the local management accountants serving the different needs of a diverse range of managers and business units. Looking at the different attitudes that central and local management accountants developed toward the CERP system, we found that although both focal groups in our analysis belonged to the same occupation, they framed the role of technology differently. Although local management accountants framed it as a tool that should enable them to draw on their local expertise to produce tailor‐made information for their local units, central management accountants saw the CERP system as a tool that allowed them to consume prefabricated "high‐quality" information to assure efficient and risk‐free accounting processes throughout the entire organization. Given this, cloud technology constitutes a risk that accounting and control processes become unduly inflexible and cumbersome at the local level. Coping with an inflexible cloud‐based system may therefore add to the list of challenges that public sector management accountants experience when trying to be(come) business partners. [ABSTRACT FROM AUTHOR]

Reconstruction of deglacial ice margins provides insights into the demise of past ice sheets and ice‐marginal lakes and helps to understand how former ice sheets responded to climate change. Here, we reconstruct deglacial Laurentide Ice Sheet margins across Manitoba (Canada), a dynamic region that in MIS 2 spanned from an inner core region of the Keewatin dome to the periphery of the ice sheet (~900 km north of the Last Glacial Maximum limit). The area was also overrun by ice flowing from both the Quebec‐Labrador dome and the Hudson Bay Ice Saddle. The surficial landscape of Manitoba contains inherited relict and palimpsest glacial landscapes, which need to be separated from deglacial features. Ice‐impounded glacial Lake Hind was present in southwest Manitoba at 13.0 cal. ka BP, meaning most of Manitoba was covered by ice at the start of the Younger Dryas. Northwest drainage of glacial Lake Agassiz in front of the Highrock Lake–Cree Lake moraine could have occurred near the end of the Younger Dryas, prior to 11.5 cal. ka BP, though the volume of the lake varies greatly depending on ice‐margin reconstructions. Our interpretation is incompatible with the hypothesis that Lake Agassiz drainage to the Arctic Ocean triggered the Younger Dryas climatic cooling. Numerous ice streams developed across central and southern Manitoba during deglaciation, including the Souris, Red River, The Pas, Hayes and Quinn Lake. The dominant ice source was from the north early in deglaciation, switching to the northeast with growth of the Hudson Bay Ice Saddle and then back to the north again with demise of the saddle. The ice‐margin ages are largely unconstrained, and thus we are unable to accurately assign climatic drivers to various ice stream events. Nonetheless, we record the development and demise of terrestrial ice streams over both hard‐bed and soft‐bed substrates. [ABSTRACT FROM AUTHOR]

This pilot study set out to examine the impact of a regular practice of yoga within schooling curricular hours on the well‐being and behaviors of atypically developing children. The design of this study was based on a qualitative hermeneutic‐phenomenology approach. The intervention consisted of 5 weekly sessions of yoga of 15 minutes each and composed of breathing techniques (pranayama), postures (pranayama), and lying down relaxation exercises (yoga nidra). The research site was a special needs school in South Auckland, New Zealand in an area characterized by poor socio‐economic living conditions with a composite range of different ethnic groups. The findings of this study suggest that a regular practice of yoga can have numerous benefits for children on the Autism Spectrum Disorder (ASD). They found yoga to be a fun‐filled and engaging experience. They improved significantly in their flexibility levels and general physical health. Emotionally, the children reported that the breathing techniques allowed them to feel calmer and more relaxed. The children felt that incorporating a regular practice of yoga within formal curriculum will be another effective tool to enhancing their overall health and well‐being. [ABSTRACT FROM AUTHOR]

We investigate defect properties in hexagonal boron nitride (hBN) which is attracting much attention as a single photon emitter. Using first-principles calculations, we find that nitrogen-vacancy defect V N has a lower energy structure in C 1 h symmetry in 1− charge state than the previously known D 3 h symmetry structure. Noting that carbon has one more valence electron than boron species, our finding naturally points to the correspondence between V N and V N C B defects with one charge state difference between them, which is indeed confirmed by the similarity of atomic symmetries, density of states, and excitation energies. Since V N C B is considered as a promising candidate for the source of single photon emission, our study suggests V N as another important candidate worth attention, with its simpler form without the incorporation of foreign elements into the host material. [ABSTRACT FROM AUTHOR]

Magnetic porous carbons were obtained from wheat flour via simple one‐step pyrolysis employing calcium chloride as activator. A series of wheat flour derived‐magnetic porous carbons (WMCs) with various pyrolysis temperature and precursor were prepared, characterized and applied for Cr(VI) removal. The results show that γ‐FeOOH and Fe3O4 were respectively obtained in the WMCs at pyrolysis temperature above and below 600 °C. While, the calcium chloride enhanced the mesoporous of WMCs and promoted the crystallization of Fe3O4. The optimal sample exhibited super Cr(VI) removal capacities of 15.53 mg g−1 and good reusability after 5 recycles in neutral solution. The Cr(VI) removal of WMCs obeys pseudo‐second‐order adsorption model and Langmuir adsorption isotherms. The enhanced adsorptivity can be attributed to the incorporation of adsorption and reduction reaction between Cr(VI) ions and Fe3O4. The density functional theory calculations also demonstrated that Cr(VI) ions is more favorable and strongly adsorbed onto Fe3O4 surface than γ‐FeOOH. [ABSTRACT FROM AUTHOR]

Background: There is limited information on risk factors for eczema in adults. Recent evidence suggests that air pollution may be associated with increased incidence of eczema in adults. We aimed to assess this possible association. Methods: Ambient air pollution exposures (distance from a major road, nitrogen dioxide [NO2], fine particulate matter with an aerodynamic diameter ≤2.5 µm [PM2.5]) were assessed for the residential address of Tasmanian Longitudinal Health Study participants at ages 43 and 53 years. Eczema incidence (onset after age 43 years), prevalence (at 53 years), and persistence were assessed from surveys, while IgE sensitization was assessed using skin prick tests. The presence or absence of eczema and sensitization was classified into four groups: no atopy or eczema, atopy alone, non‐atopic eczema, and atopic eczema. Adjusted logistic and multinomial regression models were fitted to estimate associations between ambient air pollution and eczema, and interaction by sex was assessed. Results: Of 3153 participants in both follow‐ups, 2369 had valid skin prick tests. For males, a 2.3 ppb increase in baselineNO2was associated with increased odds of prevalent eczema (OR = 1.15 [95% CI 0.98–1.36]) and prevalent atopic eczema (OR = 1.26 [1.00–1.59]). These associations were not seen in females (p for interaction = 0.08, <0.01). For both sexes, a 1.6 µg/m3 increase in PM2.5 exposure at follow‐up was associated with increased odds of aeroallergen sensitization (OR = 1.15 [1.03–1.30]). Conclusion: Increased exposure to residential ambient air pollutants was associated with an increased odds of eczema, only in males, and aeroallergen sensitization in both genders. [ABSTRACT FROM AUTHOR]

To sustain high production of alfalfa (Medicago sativa) with limited water resource in many areas, cultivars with improved water use efficiency (WUE) are urgently needed. We recently conducted greenhouse experiments and identified an alfalfa collection, River side (RS) that is naturized to the environment in the Grand River National Grassland in South Dakota, with a greater water use efficiency (WUE) under drought than other ten alfalfa accessions. To understand the mechanisms that may contribute to the higher WUE in RS, we examined physiological and morphological traits in comparison with Alfagraze (AF), a commercial variety with lower WUE under drought under greenhouse conditions. RS showed a greater reduction of water loss through transpiration despite more stomata per unit area under drought. Both RS and AF showed a reduction of stomatal aperture under drought compared to respective well‐watered plants but stomatal aperture in RS was smaller compared to AF regardless of irrigation status. Leaves of RS showed a more rapid accumulation of abscisic acid (ABA), at 14 days versus at 28 days in AF under drought. RS also closed stomata more rapidly when treated with 10 µM ABA. In addition, leaves of RS showed denser but shorter leaf hairs under drought. A correlation analysis points to a greater stomatal sensitivity to ABA in RS as a key factor contributing to its greater WUE under drought. [ABSTRACT FROM AUTHOR]