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This article discusses a study conducted on the breeding biology and aggressive behavior of Arundinicola leucocephala, a bird species found in South America. The researchers used song playback to attract breeding pairs and observed that males exhibited aggressive behavior towards their social mates instead of towards perceived male intruders. The article explores various hypotheses to explain this behavior, including the prevention of female-female conflict and sexual coercion. The study suggests that further research is needed to understand the context and conditions that influence aggression in this species. [Extracted from the article]

Rationale: The application of matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI) to murine lungs is challenging due to the spongy nature of the tissue. Lungs consist of interconnected air sacs (alveoli) lined by a single layer of flattened epithelial cells, which requires inflation to maintain its natural structure. Therefore, a protocol that is compatible with both lung instillation and high spatial resolution is essential to enable multi‐omic studies on murine lung disease models using MALDI‐MSI. Methods and results: To maintain the structural integrity of the tissue, murine lungs were inflated with 8% (w/v) gelatin for lipid MSI of fresh frozen tissues or 4% (v/v) paraformaldehyde neutral buffer for N‐glycan and peptide MSI of FFPE tissues. Tissues were sectioned and prepared for enzymatic digestion and/or matrix deposition. Glycerol‐free PNGase F was applied for N‐glycan MSI, while Trypsin Gold was applied for peptide MSI using the iMatrixSpray and ImagePrep Station, respectively. For lipid, N‐glycan and peptide MSI, α‐cyano‐4‐hydroxycinnamic acid matrix was deposited using the iMatrixSpray. MS data were acquired with 20 μm spatial resolution using a timsTOF fleX MS instrument followed by MS fragmentation of lipids, N‐glycans and peptides. For lipid MSI, trapped ion mobility spectrometry was used to separate isomeric/isobaric lipid species. SCiLS™ Lab was used to visualize all MSI data. For analyte identification, MetaboScape®, GlycoMod and Mascot were used to annotate MS fragmentation spectra of lipids, N‐glycans and tryptic peptides, respectively. Conclusions: Our protocol provides instructions on sample preparation for high spatial resolution MALDI‐MSI, MS/MS data acquisition and lipid, N‐glycan and peptide annotation and identification from murine lungs. This protocol will allow non‐biased analyses of diseased lungs from preclinical murine models and provide further insight into disease models. [ABSTRACT FROM AUTHOR]

While most flowering plant species rely on animal pollinators, approximately 10% of angiosperms utilize wind or water for pollination. Moreover, certain species can have mixed pollination system—ambophily—wherein both biotic and abiotic agents contribute to pollen transport. Upon frequent observation of damselflies (Coenagrionidae) perching on Callitriche rimosa Fassetv (Plantaginaceae), an aquatic herb with small flowers protruding above the water's surface, we hypothesized their potential contribution to the pollination of this primarily abiotically pollinated plant. Observations were conducted along the shores of a pond in southern Brazil. Damselflies were seen carrying pollen grains on their legs from C. rimosa leaves, where pollen falls from the anthers and remains exposed. Given damselflies' activity levels during summer, incidental contact with C. rimosa reproductive structures facilitates cross‐pollination. This interaction may facilitate both plants and insects, considering damselflies' dependence on freshwater bodies and use of C. rimosa leaves for perching. Accessory pollen presentation on leaves may convert occasional visitors into pollinators, expanding the pollination niche of C. rimosa. Recognizing lesser‐known pollinators like damselflies is crucial for understanding plant reproduction in diverse ecosystems. This study investigates the role of damselflies in pollinating Callitriche rimosa Fasset, traditionally considered to be abiotically pollinated. Through field observations in southern Brazil, we documented damselflies interacting with C. rimosa, potentially contributing to its pollination. Our findings shed light on the overlooked role of damselflies as pollinators in aquatic ecosystems. [ABSTRACT FROM AUTHOR]

Aim Methods Results Conclusion Many countries risk failing the Sustainable Development Goal to reduce neonatal mortality to 12 in 1000 live births before 2030, necessitating intervention. This scoping review assesses available evidence from studies implementing smartphone application‐based education and clinical decision support in neonatal emergency care in low‐ and middle‐income countries and describes applied assessment tools to highlight gaps in the current literature.A systematic search on 28 March 2024 of PubMed, Web of Science, and EMBASE identified original research papers published in peer‐reviewed journals after 2014 in English. The evaluation was based on Kirkpatrick's framework.In total, 20 studies assessing eight different smartphone applications were included. Participants found applications acceptable and feasible in 11 of 14 studies. Knowledge and/or skills were improved in 11 of 12 studies. Behaviour was assessed in 10 studies by tracking app usage. Patient outcome was assessed in four studies, focusing on perinatal mortality, Basic Newborn Care outcomes and correct assessment of newborns.Data from included studies further strengthens hope that smartphone applications can improve neonatal mortality rates in low‐ and middle‐income countries. However, further research into the effectiveness of these applications is warranted. This review highlights gaps in the current literature and provides guidance for future trials. [ABSTRACT FROM AUTHOR]

This systematic review and meta‐analysis assesses independently the acute effects of anodal and cathodal motor cortex transcranial direct current stimulation (tDCS) on athletic performance in healthy adults. Besides, it evaluates the unique and conjoint effects of potential moderators (i.e., stimulation parameters, exercise type, subjects' training status and risk of bias). Online database search was performed from inception until March 18th 2024 (PROSPERO: CRD42023355461). Forty‐three controlled trials were included in the systematic review, 40 in the anodal tDCS meta‐analysis (68 effects), and 9 (11 effects) in the cathodal tDCS meta‐analysis. Performance enhancement between pre‐ and post‐stimulation was the main outcome measure considered. The anodal tDCS effects on physical performance were small to moderate (g =.29, 95%CI [.18,.40], PI = −.64 to 1.23, I2 = 64.0%). Exercise type, training status and use of commercial tDCS were significant moderators of the results. The cathodal tDCS effects were null (g =.04, 95%CI [−.05,.12], PI = −.14 to.23, I2 = 0%), with a small to moderate heterogeneity entirely due to sampling error, thus impairing further moderator analysis. These findings hold significant implications for the field of brain stimulation and physical performance, as they not only demonstrate a small to moderate effect of acute tDCS but also identify specific categories of individuals, devices and activities that are more susceptible to improvements. By addressing the multidimensional factors influencing the mechanisms of tDCS, we also provide suggestions for future research. Results indicate a small favouring effect of anodal tDCS on athletic performance with large heterogeneity. Exercise type, training status and use of commercial tDCS significantly moderate anodal tDCS effects. Cathodal tDCS shows null effects, with small to moderate heterogeneity entirely due to sampling error. [ABSTRACT FROM AUTHOR]

Aims: To investigate how a change in body position with light‐intensity physical activity (PA) 'snacks' (LIPAS, alternate sitting and standing, walking or standing continuously) compared with uninterrupted prolonged sitting affects glucose metabolism and heart rate variability (HRV) parameters in young adults with overweight and obesity. Materials and Methods: We conducted a four‐arm randomized controlled crossover trial. The following conditions were tested during an 8‐h simulated workday: uninterrupted prolonged sitting (SIT), alternate sitting and standing (SIT‐STAND; 2.5 h total), continuous standing (STAND), and continuous walking (1.0 mph; WALK). The primary outcome was to investigate how a change in body position (alternate sitting and standing, walking or standing continuously) compared with uninterrupted sitting affects mean 8‐h glucose metabolism. Secondary outcomes included the effects on 2‐h postprandial glucose concentrations, as well as on 8‐h/24‐h heart rate and HRV parameters, in the respective study arms. Capillary blood samples were drawn from an hyperemised earlobe in the fasted state and once every hour during each trial intervention by puncturing the earlobe with a lancet and collecting 20 μL of blood (Biosen S‐Line Lab+; EKF diagnostics, Barleben, Germany). HRV was assessed for 24 h including the 8‐h intervention phase, and a home phase by means of a Holter electrocardiogram. All participants received the same standardized non‐relativised breakfast and lunch during the four trial visits. Results: Seventeen individuals (eight women, mean age 23.4 ± 3.3 years, body mass index 29.7 ± 3.8 kg/m2, glycated haemoglobin level 34.8 ± 3.1 mmol/mol [5.4 ± 0.3%], body fat 31.8 ± 8.2%) completed all four trial arms. Compared with SIT (89.4 ± 6.8 mg/dL), 8‐h mean glucose was lower in all other conditions (p < 0.05) and this was statistically significant compared with WALK (86.3 ± 5.2 mg/dL; p = 0.034). Two‐hour postprandial glucose after breakfast was approximately 7% lower for WALK compared with SIT (p = 0.002). Furthermore, significant time × condition effects on HRV parameters favouring light‐intensity walking were observed (p < 0.001). Conclusions: Replacement and interruption of prolonged sitting with light‐intensity walking showed a significant blood glucose‐lowering effect and improved HRV during an 8‐h work environment in young adults with overweight and obesity. [ABSTRACT FROM AUTHOR]

Malaria vaccination approaches using live Plasmodium parasites are currently explored, with either attenuated mosquito-derived sporozoites or attenuated blood-stage parasites. Both approaches would profit from the availability of attenuated and avirulent parasites with a reduced blood-stage multiplication rate. Here we screened gene-deletion mutants of the rodent parasite P. berghei and the human parasite P. falciparum for slow growth. Furthermore, we tested the P. berghei mutants for avirulence and resolving blood-stage infections, while preserving sporozoite formation and liver infection. Targeting 51 genes yielded 18 P. berghei gene-deletion mutants with several mutants causing mild infections. Infections with the two most attenuated mutants either by blood stages or by sporozoites were cleared by the immune response. Immunization of mice led to protection from disease after challenge with wild-type sporozoites. Two of six generated P. falciparum gene-deletion mutants showed a slow growth rate. Slow-growing, avirulent P. falciparum mutants will constitute valuable tools to inform on the induction of immune responses and will aid in developing new as well as safeguarding existing attenuated parasite vaccines. Synopsis: The two vaccines currently employed against malaria are based on a single Plasmodium antigen, require 4 shots and provide only limited protection. Attenuated whole parasite vaccines are currently in development but also require multiple injections of high numbers of parasites for protection. Here we explore genetically modified whole parasite vaccines that grow slow in the blood stage but can develop fully through the mosquito. Vaccinating mice with just a few mosquito bites leads to full protection from disease symptoms. The two vaccines currently employed against malaria are based on a single Plasmodium antigen, require 4 shots and provide only limited protection. Attenuated whole parasite vaccines are currently in development but also require multiple injections of high numbers of parasites for protection. [ABSTRACT FROM AUTHOR]

Grape berry moths, particularly Lobesia botrana Denis & Schiffermüller (Lepidoptera: Tortricidae), and vinegar flies, such as Drosophila melanogaster Meigen and Drosophila suzukii Matsumura (Diptera: Drosophilidae), are important vineyard pests, causing severe quality loss of grapes. Several arthropod taxa may be involved in the natural control of these pests. However, the role of arthropod predators in the natural control of vineyard pests remains unclear. We investigated 32 vineyards in the Palatinate region, southwest Germany, under organic and conventional management, which in both cases received either full or reduced fungicide applications (2 × 2 design). Predation of L. botrana eggs and pupae and D. melanogaster pupae on sentinel cards exposed in the vineyards was observed with infrared cameras. In total, nine predator taxa could be identified. The most dominant predator was the European earwig, Forficula auricularia L. (Dermaptera: Forficulidae), with 90% of all predation events. We conclude that F. auricularia is likely a key predator of vineyard pests, and that special attention should be paid to maintain it at high population densities. [ABSTRACT FROM AUTHOR]

Diffusion‐based tractography in the optic nerve requires sampling strategies assisted by anatomical landmark information (regions of interest [ROIs]). We aimed to investigate the feasibility of expert‐placed, high‐resolution T1‐weighted ROI‐data transfer onto lower spatial resolution diffusion‐weighted images. Slab volumes from 20 volunteers were acquired and preprocessed including distortion bias correction and artifact reduction. Constrained spherical deconvolution was used to generate a directional diffusion information grid (fibre orientation distribution‐model [FOD]). Three neuroradiologists marked landmarks on both diffusion imaging variants and structural datasets. Structural ROI information (volumetric interpolated breath‐hold sequence [VIBE]) was respectively registered (linear with 6/12 degrees of freedom [DOF]) onto single‐shot EPI (ss‐EPI) and readout‐segmented EPI (rs‐EPI) volumes, respectively. All eight ROI/FOD‐combinations were compared in a targeted tractography task of the optic nerve pathway. Inter‐rater reliability for placed ROIs among experts was highest in VIBE images (lower confidence interval 0.84 to 0.97, mean 0.91) and lower in both ss‐EPI (0.61 to 0.95, mean 0.79) and rs‐EPI (0.59 to 0.86, mean 0.70). Tractography success rate based on streamline selection performance was highest in VIBE‐drawn ROIs registered (6‐DOF) onto rs‐EPI FOD (70.0% over 5%‐threshold, capped to failed ratio 39/16) followed by both 12‐DOF‐registered (67.5%; 41/16) and nonregistered VIBE (67.5%; 40/23). On ss‐EPI FOD, VIBE‐ROI‐datasets obtained fewer streamlines overall with each at 55.0% above 5%‐threshold and with lower capped to failed ratio (6‐DOF: 35/36; 12‐DOF: 34/34, nonregistered 33/36). The combination of VIBE‐placed ROIs (highest inter‐rater reliability) with 6‐DOF registration onto rs‐EPI targets (best streamline selection performance) is most suitable for white matter template generation required in group studies. [ABSTRACT FROM AUTHOR]

Background: Botulinum neurotoxin type A (BoNT‐A) is well‐established for treatment of glabellar lines (GLs), and mostly formulated as powders requiring reconstitution for injection. The approved liquid formulation, ready‐to‐use (RTU) abobotulinumtoxinA was developed to ease injection procedures and prevent reconstitution errors. This multicenter, open‐label, Phase IV study evaluated GL treatment experience using RTU abobotulinumtoxinA versus powder BoNT‐A (onabotulinumtoxinA). Methods: Females with experience of BoNT‐A facial treatment were randomized 2:1 to GL treatment with 50 U RTU abobotulinumtoxinA (N = 99) or 20 U powder BoNT‐A (N = 51) and followed‐up for 6 months or 1 month, respectively. Assessments included: time to prepare each product for injection (primary endpoint); investigators' experience with product preparation/reconstitution; investigators' and subjects' treatment experience; safety; and for the RTU product: aesthetic improvement of GLs; subject satisfaction. Results: Compared with powder BoNT‐A, RTU abobotulinumtoxinA required statistically significantly less preparation time (mean 0:33 vs. 1:34 min: s; p < 0.0001). Investigators preferred RTU abobotulinumtoxinA over powder BoNT‐A (81% of treatment sessions) and found it allowed more time to communicate with subjects (97%). All investigators (100%) also found it easy‐to‐use, easy‐to‐learn, and that it fulfilled their expectations. Subjects found the RTU abobotulinumtoxinA treatment comfortable (91%), and through 6 months posttreatment, most reported satisfaction with their appearance (≥88%), looking natural (≥95%) and refreshed (≥80%). At Month 1, 99% of RTU‐treated subjects had investigator‐assessed improved aesthetic appearance in GLs, maintained in 76% at Month 6. No serious adverse events occurred. Conclusion: RTU abobotulinumtoxinA for GL treatment is well‐tolerated, efficacious, shows high levels of subject satisfaction throughout 6 months, saves time, and is preferred by clinicians over powder BoNT‐A. ClinicalTrials.gov Registry: NCT05277337. [ABSTRACT FROM AUTHOR]

Objective: Computer aided diagnostics (CAD) systems can automate the differentiation of maxillary sinus (MS) with and without opacification, simplifying the typically laborious process and aiding in clinical insight discovery within large cohorts. Methods: This study uses Hamburg City Health Study (HCHS) a large, prospective, long‐term, population‐based cohort study of participants between 45 and 74 years of age. We develop a CAD system using an ensemble of 3D Convolutional Neural Network (CNN) to analyze cranial MRIs, distinguishing MS with opacifications (polyps, cysts, mucosal thickening) from MS without opacifications. The system is used to find correlations of participants with and without MS opacifications with clinical data (smoking, alcohol, BMI, asthma, bronchitis, sex, age, leukocyte count, C‐reactive protein, allergies). Results: The evaluation metrics of CAD system (Area Under Receiver Operator Characteristic: 0.95, sensitivity: 0.85, specificity: 0.90) demonstrated the effectiveness of our approach. MS with opacification group exhibited higher alcohol consumption, higher BMI, higher incidence of intrinsic asthma and extrinsic asthma. Male sex had higher prevalence of MS opacifications. Participants with MS opacifications had higher incidence of hay fever and house dust allergy but lower incidence of bee/wasp venom allergy. Conclusion: The study demonstrates a 3D CNN's ability to distinguish MS with and without opacifications, improving automated diagnosis and aiding in correlating clinical data in population studies. Level of Evidence: 3 Laryngoscope, 134:3927–3934, 2024 [ABSTRACT FROM AUTHOR]

Purpose: To investigate surrogate optimisation (SO) as a modern, purely data‐driven, nonlinear adaptive iterative strategy for lens formula constant optimisation in intraocular lens power calculation. Methods: A SO algorithm was implemented for optimising the root mean squared formula prediction error (rmsPE, defined as predicted refraction minus achieved refraction) for the SRKT, Hoffer Q, Holladay, Haigis and Castrop formulae in a dataset of N = 888 cataractous eyes with implantation of the Hoya Vivinex hydrophobic acrylic aspheric lens. A Gaussian Process estimator was used as the model, and the SO was initialised with equidistant datapoints within box constraints, and the number of iterations restricted to either 200 (SRKT, Hoffer Q, Holladay) or 700 (Haigis, Castrop). The performance of the algorithm was compared to the classical gradient‐based Levenberg‐Marquardt algorithm. Results: The SO algorithm showed stable convergence after fewer than 50/150 iterations (SRKT, HofferQ, Holladay, Haigis, Castrop). The rmsPE was reduced systematically to 0.4407/0.4288/0.4265/0.3711/0.3449 dioptres. The final constants were A = 119.2709, pACD = 5.7359, SF = 1.9688, −a0 = 0.5914/a1 = 0.3570/a2 = 0.1970, C = 0.3171/H = 0.2053/R = 0.0947 for the SRKT, Hoffer Q, Holladay, Haigis and Castrop formula and matched the respective constants optimised in previous studies. Conclusion: The SO proves to be a powerful adaptive nonlinear iteration algorithm for formula constant optimisation, even in formulae with one or more constants. It acts independently of a gradient and is in general able to search within a (box) constrained parameter space for the best solution, even where there are multiple local minima of the target function. [ABSTRACT FROM AUTHOR]

This article discusses the need for a dedicated classification system for fetal intraventricular hemorrhage (IVH) and periventricular hemorrhagic venous infarction (PVHI). These conditions are major causes of mortality and morbidity in preterm newborns and can lead to long-term neurodevelopmental issues. The article highlights the differences between fetal and postnatal IVH, the risk factors associated with fetal IVH, and the use of ultrasound and MRI for diagnosis. It also emphasizes the need for further research on the outcomes of fetal IVH and the development of a classification system to improve prenatal counseling and guide treatment decisions. The authors propose a classification system based on fetal neurosonography and MRI that includes various parameters such as location, extent, and laterality of parenchymal involvement, as well as the presence of posterior fossa involvement. They suggest that this classification system could serve as a reliable prognostic tool for accurate parental counseling. The authors also outline a research timeline for the development and evaluation of this classification system. [Extracted from the article]

Pharmacogenetic variants of the steroid hormone‐metabolizing enzyme cytochrome P450 2B6 (CYP2B6) were reported to be associated with breast cancer (BC) risk and prognosis. CYP2B6 expression is inducible by estradiol (E2) but induction was demonstrated only under steroid hormone‐deprived medium conditions. Physiological conditions, however, even under endocrinological BC treatment, do not correspond to complete steroid hormone depletion. The aim of this study was to investigate the E2‐mediated CYP2B6 and CYP1B1 regulation under various steroid hormone conditions, including physiological concentrations, in human oestrogen receptor positive (T47D, MCF‐7) and negative (MDA‐MB‐231) BC cell lines. We confirm that steroid‐deprived pre‐cultivation led to CYP2B6 upregulation in T47D, but not in MCF‐7. However, when pre‐cultivated with steroid‐containing medium CYP2B6 was downregulated in T47D and MCF‐7, while the addition of physiological E2 concentrations to steroid‐deprived medium resulted in a downregulation in T47D. In contrast, CYP1B1 was never downregulated in any culture condition. Thus, we show that E2‐mediated CYP2B6 regulation in BC cells depends on steroid hormone exposure in a cell line‐specific manner. Our data indicates the importance of being careful with conclusions drawn from CYP2B6 induction findings in vitro, as we demonstrate potential influences of hormonal changes on CYP2B6 expression, which could impact steroid hormone homeostasis and, consequently, BC risk. [ABSTRACT FROM AUTHOR]

We report the syntheses of tin(II) salts of the types [L1SnX]SnX3 [L1=2,6‐{(i‐PrO)2(O)P}2C5H3N: 1, X=Cl; 2, X=Br], [L2SnCl]SnCl3 [L2=2‐{(i‐PrO)Ph(O)P}‐6‐{(i‐PrO)2(O)P}C5H3N: 3], [L3SnX]SnX3 [L3=2,6‐{MeO(O)C}2C5H3N: 4, X=Cl; 5, X=Br], [L4SnX]SnX3 [L4=2,6‐{Et2N(O)C}2C5H3N: 6, X=Cl; 7, X=Br]. These compounds were obtained by addition of SnX2 to the corresponding ligand inducing autoionization of the respective tin(II) halide. The thermal stability of 1, 3, and 4 was elucidated, giving, under ester cleavage and cyclisation, the tin(II) derivatives 8–12. The reaction of [L1SnCl]SnCl3 (1) with W(CO)4(thf)2 afforded the tungsten tetracarbonyl complex [{L1SnCl}{SnCl3}W(CO)4] (13), representing the first example in which a tin(II) stannate anion and a tin(II) stannylium cation simultaneously coordinate to a transition metal centre. The compounds were characterized by single crystal X‐ray diffraction analyses and in part by elemental analyses, IR and NMR spectroscopy, electrospray ionization mass spectrometry. DFT calculations accompany the experimental work. [ABSTRACT FROM AUTHOR]

The 4th Davos Declaration was developed during the Global Allergy Forum in Davos which aimed to elevate the care of patients with atopic dermatitis (AD) by uniting experts and stakeholders. The forum addressed the high prevalence of AD, with a strategic focus on advancing research, treatment, and management to meet the evolving challenges in the field. This multidisciplinary forum brought together top leaders from research, clinical practice, policy, and patient advocacy to discuss the critical aspects of AD, including neuroimmunology, environmental factors, comorbidities, and breakthroughs in prevention, diagnosis, and treatment. The discussions were geared towards fostering a collaborative approach to integrate these advancements into practical, patient‐centric care. The forum underlined the mounting burden of AD, attributing it to significant environmental and lifestyle changes. It acknowledged the progress in understanding AD and in developing targeted therapies but recognized a gap in translating these innovations into clinical practice. Emphasis was placed on the need for enhanced awareness, education, and stakeholder engagement to address this gap effectively and to consider environmental and lifestyle factors in a comprehensive disease management strategy. The 4th Davos Declaration marks a significant milestone in the journey to improve care for people with AD. By promoting a holistic approach that combines research, education, and clinical application, the Forum sets a roadmap for stakeholders to collaborate to improve patient outcomes in AD, reflecting a commitment to adapt and respond to the dynamic challenges of AD in a changing world. [ABSTRACT FROM AUTHOR]

The role of large frugivores in the dispersal of tropical trees has been well‐studied, whereas the importance of the world's largest rodents for plant dispersal has previously been ignored. We studied plant dispersal by gut passage (endozoochory) in Neotropical wetlands by the largest extant rodent, the capybara Hydrochoerus hydrochaeris. We compared it with another of the world's 10 largest rodents, the nutria or coypu Myocastor coypus.We collected 96 faecal samples (50 capybara, 46 nutria) from 10 sites within the Taim Ramsar site in southern Brazil, and extracted intact propagules from 10 g subsamples. Propagules from 27 plant taxa (24 angiosperms, and three aquatic ferns) were recovered, representing 13 plant families. Whole Spirodela (duckweed) plants were dispersed. Seeds from 14 angiosperm taxa were germinated in the laboratory.Taxonomic composition of propagules was significantly different for the two mammals, but propagule size was not. Capybara dispersed more terrestrial propagules per sample, and nutrias more aquatic propagules. When total faecal production was taken into account, an estimated 1025 propagules (including 133 fern spores) were dispersed by each capybara per day, compared with 691 propagules for nutrias (including 566 fern spores). Capybaras have larger home ranges and likely dispersed plants over a greater distance.Synthesis: This is the first demonstration that rodents disperse aquatic pteridophytes as well as flowering plants. Our results suggest that capybaras and nutrias play important, complementary roles in dispersing a variety of aquatic and terrestrial plant species within Neotropical wetlands. Our study indicates that the role of herbivorous mammals as vectors of plants with small seeds and non‐fleshy fruits has been overlooked in the neotropics and that defaunation of large herbivores can have negative consequences, in a manner similar to that previously highlighted for frugivores. [ABSTRACT FROM AUTHOR]

The microbiome field has grown significantly in the past decade, and published studies have provided an overview of the microorganisms inhabiting the skin of companion animals. With the continued growth and interest in this field, concerns have been raised regarding sample collection methods, reagent contamination, data processing and environmental factors that may impair data interpretation (especially as related to low‐biomass skin samples). In order to assure transparency, it is important to report all steps from sample collection to data analysis, including use of proper controls, and to make sequence data and sample metadata publicly available. Whilst interstudy variation will continue to exist, efforts to standardise methods will reduce confounding variables, and allow for reproducibility and comparability of results between studies. Companion animal microbiome studies often include clinical cases, and small sample sizes may result in lack of statistical significance within small datasets. The ability to combine results from standardised studies through meta‐analyses would mitigate the limitations of these smaller studies, providing for more robust interpretation of results which could then inform clinical decisions. In this narrative review, we aim to present considerations for designing a study to evaluate the skin microbiome of companion animals, from conception to data analysis. [ABSTRACT FROM AUTHOR]

Background and purpose: This systematic review examines the effectiveness of motivational interviewing (MI) on medication adherence for preventing recurrent stroke and transient ischemic attack (TIA). Methods: MEDLINE (via PubMed), CINAHL, PsycINFO, CENTRAL, and ClinicalTrials.gov were searched from inception to 12 June 2023. Randomized controlled trials comparing MI with usual care or interventions without MI in participants with any stroke type were identified and summarized descriptively. Primary outcome was medication adherence. Secondary outcomes were quality of life (QoL) and different clinical outcomes. We assessed risk of bias with RoB 2 (revised Cochrane risk‐of‐bias tool) and intervention complexity with the iCAT_SR (intervention Complexity Assessment Tool for Systematic Reviews). Results: We screened 691 records for eligibility and included four studies published in five articles. The studies included a total of 2751 participants, and three were multicentric. Three studies had a high risk of bias, and interventions varied in complexity. Two studies found significantly improved medication adherence, one at 9 (96.9% vs. 88.2%, risk ratio = 1.098, 95% confidence interval = 1.03–1.17) and one at 12 months (97.0% vs. 95.0%, p = 0.026), but not at other time points, whereas two other studies reported no significant changes. No significant differences were found in QoL or clinical outcomes. Conclusions: Evidence on MI appears inconclusive for improving medication adherence for recurrent stroke and TIA prevention, with no benefits on QoL and clinical outcomes. There is a need for robustly designed studies and process evaluations of MI as a complex intervention for people with stroke. Registration: PROSPERO (CRD42023433284). [ABSTRACT FROM AUTHOR]

INTRODUCTION: Dementia risk scores constitute promising surrogate outcomes for lifestyle interventions targeting cognitive function. We investigated whether dementia risk, assessed using the LIfestyle for BRAin health (LIBRA) index, was reduced by the AgeWell.de intervention. METHODS: Secondary analyses of the AgeWell trial, testing a multicomponent intervention (including optimization of nutrition, medication, and physical, social, and cognitive activity) in older adults with increased dementia risk. We analyzed data from n = 461 participants with complete information on risk/protective factors comprised by LIBRA at the 24‐month follow‐up. Intervention effects on LIBRA and LIBRA components were assessed using generalized linear models. RESULTS: The intervention reduced LIBRA scores, indicating decreased dementia risk at follow‐up (b = –0.63, 95% confidence interval [CI]: –1.14, –0.12). Intervention effects were particularly due to improvements in diet (odds ratio [OR]: 1.60, 95% CI: 1.16, 2.22) and hypertension (OR: 1.61, 95% CI: 1.19, 2.18). DISCUSSION: The AgeWell.de intervention reduced dementia risk. However, several risk factors did not improve, possibly requiring more intensive interventions. Highlights: The AgeWell.de intervention reduced dementia risk according to LIfestyle for BRAin health (LIBRA) scores.Beneficial effects on LIBRA are mainly due to changes in diet and blood pressure.A pragmatic lifestyle intervention is apt to reduce dementia risk in an at‐risk population. [ABSTRACT FROM AUTHOR]

Background: To compare results from different corneal astigmatism measurement instruments; to reconstruct corneal astigmatism from the postimplantation spectacle refraction and toric intraocular lens (IOL) power; and to derive models for mapping measured corneal astigmatism to reconstructed corneal astigmatism. Methods: Retrospective single centre study involving 150 eyes treated with a toric IOL (Alcon SN6AT, DFT or TFNT). Measurements included IOLMaster 700 keratometry (IOLMK) and total keratometry (IOLMTK), Pentacam keratometry (PK) and total corneal refractive power in 3 and 4 mm zones (PTCRP3 and PTCRP4), and Aladdin keratometry (AK). Regression‐based models mapping the measured C0 and C45 components (Alpin's method) to reconstructed corneal astigmatism were derived. Results: Mean C0 components were 0.50/0.59/0.51 dioptres (D) for IOLMK/PK/AK; 0.2/0.26/0.31 D for IOLMTK/PTCRP3/PTCRP4; and 0.26 D for reconstructed corneal astigmatism. All corresponding C45 components ranged around 0. The prediction models had main diagonal elements lower than 1 with some crosstalk between C0 and C45 (nonzero off‐diagonal elements). Root‐mean‐squared residuals were 0.44/0.45/0.48/0.51/0.50/0.47 D for IOLMK/IOLMTK/PK/PTCRP3/PTCRP4/AK. Conclusions: Results from the different modalities are not consistent. On average IOLMTK/PTCRP3/PTCRP4 match reconstructed corneal astigmatism, whereas IOLMK/PK/AK show systematic C0 offsets of around 0.25 D. IOLMTK/PTCRP3/PTCRP4. Prediction models can reduce but not fully eliminate residual astigmatism after toric IOL implantation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Myzus persicae, a serious sap‐sucking pest of a large variety of host plants in agriculture, is traditionally controlled using chemical insecticides but there is interest in using biopesticides as restrictions are increasingly placed on the use of broad‐spectrum pesticides. RESULTS: Here, we show that in Petri dish experiments, high concentrations of the fungal entomopathogen Beauveria bassiana led to rapid mortality of M. persicae, although at a low concentration (1 × 104 conidia mL−1) there is a hormetic effect in which survival and fecundity are enhanced. Hormetic effects persisted across a generation with reduced development time and increased fecundity in the offspring of M. persicae exposed to B. bassiana. The whole‐plant experiment points to a hormetic effect being detected in two out of three tested lines. The impact of these effects might also depend on whether M. persicae was transinfected with the endosymbiont Rickettsiella viridis, which decreases fecundity and survival compared with aphids lacking this endosymbiont. This fecundity cost was ameliorated in the generation following exposure to the entomopathogen. CONCLUSION: Although B. bassiana is effective in controlling M. persicae especially at higher spore concentrations, utilization of this entomopathogen requires careful consideration of hormetic effects at lower spore concentrations, and further research to optimize its application for sustainable agriculture is recommended. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Background: The COVID‐19 pandemic adversely affected children's mental health (MH) and changed patterns of MH emergency department (ED) utilization. Our objective was to assess how pediatric MH ED visits during the COVID‐19 pandemic differed from expected prepandemic trends. Methods: We retrospectively studied MH ED visits by children 5 to <18 years old at nine U.S. hospitals participating in the Pediatric Emergency Care Applied Research Network Registry from 2017 to 2022. We described visit length by time period: prepandemic (January 2017–February 2020), early pandemic (March 2020–December 2020), midpandemic (2021), and late pandemic (2022). We estimated expected visit rates from prepandemic data using multivariable Poisson regression models. We calculated rate ratios (RRs) of observed to expected visits per 30 days during each pandemic time period, overall and by sociodemographic and clinical characteristics. Results: We identified 175,979 pediatric MH ED visits. Visit length exceeded 12 h for 7.3% prepandemic, 8.4% early pandemic, 15.0% midpandemic, and 19.2% late pandemic visits. During the early pandemic, observed visits per 30 days decreased relative to expected rates (RR 0.80, 95% confidence interval [CI] 0.78–0.84), were similar to expected rates during the midpandemic (RR 1.01, 95% CI 0.96–1.07), and then decreased below expected rates during the late pandemic (RR 0.92, 95% CI 0.86–0.98). During the late pandemic, visit rates were higher than expected for females (RR 1.10, 95% CI 1.02–1.20) and for bipolar disorders (RR 1.83, 95% CI 1.38–2.75), schizophrenia spectrum disorders (RR 1.55, 95% CI 1.10–2.59), and substance‐related and addictive disorders (RR 1.50, 95% CI 1.18–2.05). Conclusions: During the late pandemic, pediatric MH ED visits decreased below expected rates; however, visits by females and for specific conditions remained elevated, indicating a need for increased attention to these groups. Prolonged ED visit lengths may reflect inadequate availability of MH services. [ABSTRACT FROM AUTHOR]

Decreased monocytic HLA‐DR expression is the most studied biomarker of immune competency in critically ill and autoimmune disease patients. However, the underlying regulatory mechanisms remain largely unknown. One probable HLA‐DR dysregulation is through microRNAs. The aim of this study was to investigate the effects of specific microRNAs on HLA‐DR expression in human monocytic cells. Four up‐ and four down‐HLA‐DR‐regulating microRNAs were identified, with hsa‐miR‐let‐7f‐2‐3p showing the most significant upregulation and hsa‐miR‐567 and hsa‐miR‐3972 downregulation. Anti‐inflammatory glucocorticoid medication Dexamethasone‐decreased HLA‐DR was significantly restored by hsa‐miR‐let‐7f‐2‐3p and hsa‐miR‐5693. Contrarily, proinflammatory cytokines IFN‐γ and TNF‐α‐increased HLA‐DR were significantly reversed by hsa‐miR‐567. Clinically, paired plasma samples from patients before and one day after cardiac surgery revealed up‐regulated expression of hsa‐miR‐5693, hsa‐miR‐567, and hsa‐miR‐3972, following the major surgical trauma. In silico approaches were applied for functional microRNA‐mRNA interaction prediction and candidate target genes were confirmed by qPCR analysis. In conclusion, novel monocytic HLA‐DR microRNA modulators were identified and validated in vitro. Moreover, both the interaction between the microRNAs and anti‐ and proinflammatory molecules and the up‐regulated microRNAs identified in cardiac surgery highlight the potential clinical relevance of our findings. [ABSTRACT FROM AUTHOR]

In the face of a crisis, understanding the possible determinants of economic performance can help managers act effectively. This paper examines the relationship between pre-shock firm openings and closings and regional economic resilience. Data from 294 municipalities in Brazil were analysed using descriptive statistics, ordinary least squares and quantile regression. The results show that the regions with the highest rates of firm births were also the most resilient to the COVID-19 shock. On the other hand, the least resilient regions were those with the highest pre-crisis enterprise death rates. These findings point to the promotion of entrepreneurship and institutional support for business activity as a means of enhancing the ability to withstand or recover from shocks. [ABSTRACT FROM AUTHOR]

In the field of anticancer therapy study it is of great interest to find effective G‐quadruplex ligands which may be of potential use in medical treatment or cancer prevention. Since among the compounds of natural origin, flavonoids have attracted notable attention because of their unique properties and promising therapeutic applications, an interesting question was to identify the flavonoid structural features that could provide effective binding properties toward G‐quadruplex. By using electrospray ionization mass spectrometry, followed by the survival yield method, it has been shown that the flavonoid molecules which contain an available C4=O carbonyl group form more stable adducts with G‐tetrads than the other ones. Molecular docking has shown that C4=O carbonyl group can be a source of hydrogen bonds and/or π‐stacking interactions. Therefore, the flavonoid molecules which contain an available C4=O carbonyl group can be regarded as good binders of G‐quadruplexes. [ABSTRACT FROM AUTHOR]

Functional cure for chronic hepatitis B (CHB) remains challenging due to the lack of direct intervention methods for hepatic inflammation. Multi‐omics research offers a promising approach to understand hepatic inflammation mechanisms in CHB. A Bayesian linear model linked gene expression with clinical parameters, and population‐specific expression analysis (PSEA) refined bulk gene expression into specific cell types across different clinical phases. These models were integrated into our analysis of key factors like inflammatory cells, immune activation, T cell exhaustion, chemokines, receptors, and interferon‐stimulated genes (ISGs). Validation through multi‐immune staining in liver specimens from CHB patients bolstered our findings. In CHB patients, increased gene expression related to immune cell activation and migration was noted. Marker genes of macrophages, T cells, immune‐negative regulators, chemokines, and ISGs showed a positive correlation with serum alanine aminotransferase (ALT) levels but not hepatitis B virus DNA levels. The PSEA model confirmed T cells as the source of exhausted regulators, while macrophages primarily contributed to chemokine expression. Upregulated ISGs (ISG20, IFI16, TAP2, GBP1, PSMB9) in the hepatitis phase were associated with T cell and macrophage infiltration and positively correlated with ALT levels. Conversely, another set of ISGs (IFI44, ISG15, IFI44L, IFI6, MX1) mainly expressed by hepatocytes and B cells showed no correlation with ALT levels. Our study presents a multi‐omics analysis integrating bulk transcriptomic, single‐cell sequencing data, and clinical data from CHB patients to decipher the cause of intrahepatic inflammation in CHB. The findings confirm that macrophages secrete chemokines like CCL20, recruiting exhausted T cells into liver tissue; concurrently, hepatocyte innate immunity is suppressed, hindering the antiviral effects of ISGs. Highlights: This study integrates liver bulk transcriptomic data, single‐cell sequencing data, and clinical data to analyze the factors inducing hepatic inflammation in chronic hepatitis B from a multi‐omics perspective by Bayesian regression.Macrophages secrete chemokines like CCL20 and CXCL8 to recruit immune‐exhausted T lymphocytes (CTLA4, TIGIT) into liver tissue.Innate immunity within hepatocytes is suppressed, impeding interferon‐stimulated genes from initiating antiviral effects.Activation of innate immune pathways in infiltrating T cells and macrophages further exacerbates inflammation formation. [ABSTRACT FROM AUTHOR]

Background: Intralymphatic immunotherapy (ILIT) represents a promising novel approach treating allergic diseases. However, no standardized procedures or recommendations have been established or reported, despite the recognized fact that treatment efficacy relies on the ability to inject the allergen intranodally. Objective: We aim to provide a critical appraisal of ILIT as a method of allergen immunotherapy and to deliver practical recommendations for accurate ILIT. Methods: One hundred and seventy‐three ILIT injections were performed in 28 (47%) women and 32 (53%) men with median age of 29 years (21–59). The injections were ultrasound‐guided and recorded for retrospective analysis with respect to injection location, needle visibility, medication release, and patient characteristics. Results: The results show that the correct positioning of the needle within the lymph node (LN) was most critical. If the whole length of the needle bevel was not inserted into the LN, substance backflush into the interstitium was observed. Selecting a more superficial LN and inserting the needle at a smaller angle towards the LN significantly improved needle visibility in the ultrasound. Longitudinal results showed that continuous practice significantly correlated with improved needle visibility and more accurate ILIT injections. Conclusion: Based on our results and practical experience, we propose several recommendations for LN selection and the correct handling of ultrasound probe and needle. We are confident that ILIT standardization and training will be important as to meet the goals of good safety and efficacy of ILIT. [ABSTRACT FROM AUTHOR]

The brown planthopper (BPH, Nilaparvata lugens) is a notorious sap‐sucking insect pest that damages rice (Oryza sativa) plants throughout Asia. During BPH feeding, saliva enters rice plant tissues, whereas during oviposition egg‐associated secretions (EAS) are deposited in damaged plant tissue. Dynamic changes in rice to planthopper salivary effectors have been widely reported. However, the effects of EAS from planthopper on rice immunity remains largely unexplored. In this study, we found that both infestation of rice by gravid BPH female adults and treatment with the EAS elicited a strong and rapid accumulation of jasmonic acid (JA), JA‐isoleucine, and hydrogen peroxide in rice. EAS enhanced plant defenses not only in rice but also in tobacco, and these impaired the performance of BPH on rice, as well as the performance of aphids and whiteflies on tobacco. High‐throughput proteome sequencing of EAS led to 110 proteins being identified and 53 proteins with 2 or more unique peptides being detected. Some proteins from BPH EAS were also found in the salivary proteome from herbivores, suggesting potential evolutionary conservation of effector functions across feeding and oviposition; however, others were only identified in EAS, and these are likely specifically related to oviposition. These findings point to novel proteins affecting interactions between planthoppers and rice during oviposition, providing an additional source of information for effector studies. [ABSTRACT FROM AUTHOR]

Additive manufacturing is the state‐of‐the‐art method for producing complex ceramic parts. For cellular materials, the freedom of design is particularly advantageous, as pore networks and shapes can be tailored. Herein, the direct printing of complex parts with high porosity using the cost‐effective manufacturing method of Liquid Crystal Display‐based stereolithography on low‐cost printers is determined. It is investigated how the architecture of the alumina Kelvin cells topology, their fabrication process, the printing parameters, and the microstructure affect the accuracy and mechanical properties of the printed samples. A notable reduction to a strut thickness of Kelvin cells down to 0.20 mm is achieved corresponding to a reduction of 1.5 compared with the state‐of‐the‐art literature (min. 0.35 mm). Ultrahigh porosities between 89.5% and 97.2% and a maximum compressive strength of 1.84 ± 0.17 MPa are reached due to the dense struts of the structure. Low‐cost and simple vat ceramic photopolymerization with high accuracy printing proves to be a promising candidate for the rapid and cost‐effective fabrication of highly porous and complex ceramic structures. [ABSTRACT FROM AUTHOR]

Purpose: Pain is a common symptom following proximal femoral fractures (PFF), however, information on its treatment in terms of agents and type of use (scheduled vs. pro re nata [PRN]) is scarce. The main objective of this study was to examine pain medication regimens according to pain intensity following PFF. Furthermore, we explored the utilization of medication plans. Methods: The "ProFem"‐study on healthcare provision, functional ability, and quality of life after PFF is a German population‐based prospective cohort study based on statutory health insurance data and individually linked survey data from different time points including information on the currently used medication. This present analysis refers to the participants' baseline interviews (about 3 months following PFF) conducted from 2018 to 2019 in the participants' private surroundings. Results: The study population comprised 444 participants (mean age: 81.2 years, 71.0% female). Half of them reported high intensity pain, and the mean value for the EuroQol visual analogue scale was 50.8. Most commonly used analgesics were metamizole and tilidine/naloxone. Among participants with high intensity pain, 21.9% received only PRN pain medication and 17.2% no pain medication at all. Overall, 61.5% of participants presented any (printed) medication plan and only 25.2% a "federal standardized medication plan" (BMP). Conclusion: As a substantial number of patients reports high intensity pain about 3 months following a PFF, the large proportion of those receiving no or only PRN pain medication raises questions regarding the appropriateness of the therapy. The overall low utilization of the BMP indicates potential for improvement. [ABSTRACT FROM AUTHOR]

Objective: Perineuronal nets (PNN) are specialized extracellular matrix (ECM) components of the central nervous system, frequently accumulating at the surface of inhibitory GABAergic interneurons. While an altered distribution of PNN has been observed in neurological disorders including Alzheimer's disease, schizophrenia and epilepsy, their anatomical distribution also changes during physiological brain maturation and aging. Such an age‐dependent shift was experimentally associated also with hippocampal engram formation during brain maturation. Our aim was to histopathologically assess PNN in the hippocampus of adult and pediatric patients with temporal lobe epilepsy (TLE) compared to age‐matched post‐mortem control subjects and to compare PNN‐related changes with memory impairment observed in our patient cohort. Methods: Sixty‐six formalin‐fixed and paraffin‐embedded tissue specimens of the human hippocampus were retrieved from the European Epilepsy Brain Bank. Twenty‐nine patients had histopathologically confirmed hippocampal sclerosis (HS), and eleven patients suffered from TLE without HS. PNN were immunohistochemically visualized using an antibody directed against aggrecan and manually counted from hippocampus subfields and the subiculum. Results: PNN density increased with age in both human controls and TLE patients. However, their density was significantly higher in all HS patients compared to age‐matched controls. Intriguingly, TLE patients presented presurgically with better memory when their hippocampal PNN density was higher (p < 0.05). Significance: Our results were compatible with age‐dependent ECM specialization in the human hippocampus and its precocious aging in the epileptic condition. These observations confirm recent experimental animal models and also support the notion that PNN play a role in memory formation in the human brain. Plain Language Summary: "Perineuronal nets" (PNN) are a specialized compartment of the extracellular matrix (ECM), especially surrounding highly active neurons of the mammalian brain. There is evidence that PNN play a role in memory formation, brain maturation, and in some pathologies like Alzheimer's disease, schizophrenia or epilepsy. In this study, we investigated the role of PNN in patients suffering from drug‐resistant focal epilepsy compared to controls. We found that with increasing age, more neurons are surrounded by PNN. Similarly, all epilepsy patients but especially patients with better memory performance also had more PNN. This study raises further interest in studying ECM molecules in the human brain under physiological and pathophysiological conditions. [ABSTRACT FROM AUTHOR]

Objective: Structured transition of adolescents and young adults with a chronic endocrine disease from paediatric to adult care is important. Until now, no data on time and resources required for the necessary components of the transition process and the associated costs are available. Design, Patients and Measurements: In a prospective cohort study of 147 patients with chronic endocrinopathies, for the key elements of a structured transition pathway including (i) assessment of patients' disease‐related knowledge and needs, (ii) required education and counselling sessions, (iii) compiling an epicrisis and a transfer appointment of the patient together with the current paediatric and the future adult endocrinologist resource consumption and costs were determined. Results: One hundred and forty‐three of 147 enroled patients (97.3%) completed the transition pathway and were transferred to adult care. The mean time from the decision to start the transition process to the final transfer consultation was 399 ± 159 days. Transfer consultations were performed in 143 patients, including 128 patients jointly with the future adult endocrinologist. Most consultations were performed by a multidisciplinary team consisting of a paediatric and adult endocrinologist, psychologist, nurse, and a social worker acting also as a case manager with a median of three team members and lasted 87.6 ± 23.7 min. The mean cumulative costs per patient of all key elements were 519 ± 206 Euros. In addition, costs for case management through the transition process were 104.8 ± 28.0 Euros. Conclusions: Using chronic endocrine diseases as an example, it shows how to calculate the time and cost of a structured transition pathway from paediatric to adult care, which can serve as a starting point for sustainable funding for other chronic rare diseases. [ABSTRACT FROM AUTHOR]

Macrophages sense pathogens and orchestrate specific immune responses. Stimulus specificity is thought to be achieved through combinatorial and dynamical coding by signaling pathways. While NFκB dynamics are known to encode stimulus information, dynamical coding in other signaling pathways and their combinatorial coordination remain unclear. Here, we established live-cell microscopy to investigate how NFκB and p38 dynamics interface in stimulated macrophages. Information theory and machine learning revealed that p38 dynamics distinguish cytokine TNF from pathogen-associated molecular patterns and high doses from low, but contributed little to information-rich NFκB dynamics when both pathways are considered. This suggests that immune response genes benefit from decoding immune signaling dynamics or combinatorics, but not both. We found that the heterogeneity of the two pathways is surprisingly uncorrelated. Mathematical modeling revealed potential sources of uncorrelated heterogeneity in the branched pathway network topology and predicted it to drive gene expression variability. Indeed, genes dependent on both p38 and NFκB showed high scRNAseq variability and bimodality. These results identify combinatorial signaling as a mechanism to restrict NFκB-AND-p38-responsive inflammatory cytokine expression to few cells. Synopsis: Dual reporter live macrophage imaging reveals that MAPK p38 and NFκB dynamics encode stimulus information but decoding both does not increase stimulus-specificity further. Their poorly correlated single-cell activities render AND-gate genes, encoding cytokines, particularly variable. A workflow is established for simultaneous MAPK p38 and NFκB live cell imaging in primary mouse macrophages. While MAPK p38 dynamics encode stimulus information, they contribute little to information-rich NFκB dynamics. Stimulus-specificity of innate immune response genes may thus be achieved by decoding NFκB dynamics or NFκB and p38 combinatorics, but no further gain is achieved from decoding both. NFκB and p38 activities are poorly correlated across single cells, rendering AND gate genes, often encoding cytokines, particularly variable. Dual reporter live macrophage imaging reveals that MAPK p38 and NFκB dynamics encode stimulus information but decoding both does not increase stimulus-specificity further. Their poorly correlated single-cell activities render AND-gate genes, encoding cytokines, particularly variable. [ABSTRACT FROM AUTHOR]

Conceptual density functional theory (CDFT) and the quantum reactivity descriptors stemming from it have proven to be valuable tools for understanding the chemical behavior of molecules. This article is presented as being intrinsically of dual character. In a first part, it briefly reviews, in a deliberately didactical way, the main ensembles in CDFT, while the second half presents two additional ensembles, where the chemical hardness acts as a natural variable, and their respective reactivity descriptors. The evaluation of these reactivity descriptors on common organic chemical reagents are presented and discussed. [ABSTRACT FROM AUTHOR]

Background Aims Methods Results Conclusions The current transformation of the entire energy system leads to a large‐scale expansion of extra‐high‐voltage underground transmission lines (UTL). Knowledge of the impact on soil temperature and soil moisture dynamics is fundamental for environmental evaluation.We investigated the impact of an existing 320 kV underground cable in continuous operation on soil temperature and moisture dynamics.A soil‐monitoring programme was established at four study sites in Western Germany. Data were continuously recorded in soil up to 120 cm depth using soil sensors over a period of 1 year.Soil warming was in a range of 0.6 K in the topsoil, approx. 1–1.3 K in the rooting zone and 1.7 K in the subsoil at 120 cm depth and was restricted mainly to the immediate vicinity of the cable route. Likewise, the impact on soil moisture dynamics was on average in a range of −1.00 wt.‐% in 0–60 cm depth and −2.45 wt. 2‐% in the subsoil relative to control. Although at a calculated maximum load capacity of 100% in regular operation, soil warming might remain moderate, with 1.5 K in the topsoil, 2.3–3.1 K in the rooting zone and 4.1 K in the subsoil.It is assumed that the reasons for the low‐to‐moderate influence of the UTL are to be found in the operational cable load (on average 65%), heat loss of cables (approx. 12 W m−1 per cable) and the quality of the imbedding material for the cables. [ABSTRACT FROM AUTHOR]

Adverse childhood experiences (ACEs) are recognized as significant precursors to delinquency. However, to date, no studies have explicitly examined the interconnectedness of ACEs, residency in rural or urban/suburban areas, and delinquent behavior to discern potential variations among youths residing in these distinct areas. This study aims to address this important gap in existing literature. Data from the 2022 Florida Youth Substance Abuse Survey (FYSAS), comprising a statewide representative sample of students enrolled in public high schools in Florida (N = 23,078), were analyzed using negative binominal regression models. Our findings demonstrate a significant correlation between ACEs and delinquent behavior, with notable variations in this association among youths residing in rural versus urban/suburban environments. In particular, the impact of ACEs on the anticipated frequency of delinquent behaviors appears to be marginally greater among adolescents residing in urban/suburban locales compared to their counterparts in rural areas. Implications are provided. [ABSTRACT FROM AUTHOR]

Sulfurous acid (H2SO3) is known to be thermodynamically instable decomposing into SO2 and H2O. All attempts to detect this elusive acid in solution failed up to now. Reported H2SO3 formation from an experiment carried out in a mass spectrometer as well as results from theoretical calculations, however, indicated a possible kinetic stability in the gas phase. Here, it is shown experimentally that H2SO3 is formed in the OH radical‐initiated gas‐phase oxidation of methanesulfinic acid (CH3S(O)OH) at 295±0.5 K and 1 bar of air with a molar yield of 53-17+7 ${{53}_{-17}^{+\ 7}}$ %. Further main products are SO2, SO3 and methanesulfonic acid. CH3S(O)OH represents an important intermediate product of dimethyl sulfide oxidation in the atmosphere. Global modeling predicts an annual H2SO3 production of ∼8 million metric tons from the OH+CH3S(O)OH reaction. The investigated H2SO3 depletion in the presence of water vapor results in k(H2O+H2SO3) <3×10−18 cm3 molecule−1 s−1, which indicates a lifetime of at least one second for atmospheric humidity. This work provides experimental evidence that H2SO3, once formed in the gas phase, is kinetically stable enough to allow its characterization and subsequent reactions. [ABSTRACT FROM AUTHOR]

Schweflige Säure (H2SO3) ist dafür bekannt, dass sie thermodynamisch instabil ist und in SO2 und H2O zerfällt. Alle Versuche, diese schwer zugängliche Säure in Lösung nachzuweisen, sind bisher gescheitert. Berichte über die Bildung von H2SO3 ausgehend von Experimenten in einem Massenspektrometer sowie Ergebnisse aus theoretischen Berechnungen deuten jedoch auf eine mögliche kinetische Stabilität in der Gasphase hin. Hier wird experimentell gezeigt, dass H2SO3 bei der OH Radikal initiierten Gasphasenoxidation von Methansulfinsäure (CH3S(O)OH) bei 295±0,5 K und 1 bar Luft mit einer molaren Ausbeute von 53-17+7 ${{53}_{-17}^{+{\rm \ }7}}$ % entsteht. Weitere Hauptprodukte sind SO2, SO3 und Methansulfonsäure. CH3S(O)OH ist ein wichtiges Zwischenprodukt bei der Oxidation von Dimethylsulfid in der Atmosphäre. Globale Modellierungen zeigen eine jährliche H2SO3 Produktion von ∼8 Millionen Tonnen aus der Reaktion OH+CH3S(O)OH. Der untersuchte H2SO3 Zerfall in Anwesenheit von Wasserdampf ergab k(H2O+H2SO3) <3×10−18 cm3 Molekül−1 s−1, was auf eine Lebensdauer von mindestens einer Sekunde für atmosphärische Feuchtebedingungen hinweist. Diese Arbeit liefert den experimentellen Beweis dafür, dass H2SO3 nach seiner Bildung in der Gasphase kinetisch ausreichend stabil ist, um seine Charakterisierung und nachfolgende Reaktionen zu gestatten. [ABSTRACT FROM AUTHOR]

Endosymbionts are widespread in arthropods, living in host cells with effects that extend from parasitic to mutualistic. Newly acquired endosymbionts tend to be parasitic, but vertical transmission favors coevolution toward mutualism, with hosts sometimes developing dependency. Endosymbionts negatively affecting host fitness may still spread by impacting host reproductive traits, referred to as reproductive "manipulation," although costs for hosts are often assumed rather than demonstrated. For cytoplasmic incompatibility (CI) that involves endosymbiont‐mediated embryo death, theory predicts directional shifts away from "manipulation" toward reduced CI strength; moreover, CI‐causing endosymbionts need to increase host fitness to initially spread. In nature, endosymbiont–host interactions and dynamics are complex, often depending on environmental conditions and evolutionary history. We advocate for capturing this complexity through appropriate datasets, rather than relying on terms like "manipulation." Such imprecision can lead to the misclassification of endosymbionts along the parasitism–mutualism continuum. [ABSTRACT FROM AUTHOR]

Wolbachia continue to be reported in species previously thought to lack them, particularly Aedes aegypti mosquitoes. The presence of Wolbachia in this arbovirus vector is considered important because releases of mosquitoes with transinfected Wolbachia are being used around the world to suppress pathogen transmission and these efforts depend on a lack of Wolbachia in natural populations of this species. We previously assessed papers reporting Wolbachia in natural populations of Ae. aegypti and found little evidence that seemed convincing. However, since our review, more and more papers are emerging on Wolbachia detections in this species. Our purpose here is to evaluate these papers within the context of criteria we previously established but also new criteria that include the absence of releases of transinfections within the local areas being sampled which has contaminated natural populations in at least one case where novel detections have been reported. We also address the broader issue of Wolbachia detection in other insects where similar issues may arise which can affect overall estimates of this endosymbiont more generally. We note continuing shortcomings in papers purporting to find natural Wolbachia in Ae. aegypti which are applicable to other insects as well. [ABSTRACT FROM AUTHOR]

Forensic science not only provides evidence to support criminal investigations, but also contributes to knowledge surrounding criminal phenomena. Forensic scientists are in the unique position to strategically combine information gained from traces to create intelligence that can assist the security space in keeping our communities safe. Research into the use of traces for more than just criminal investigations, with a focus on the greater "utility" of a trace, has pointed to the broader problem‐solving potential of traces when more fully exploited. Alongside this, there has continued to be an exponential increase of the use of biological traces for criminal investigations, in particular the collection of trace DNA specimens. The potential for identification, among other information, trace DNA can provide is vast, however much of the previous research has predominately focused on recovery rates which does not provide a holistic view of the value of trace DNA. This paper summarizes the current knowledge on the utility of trace DNA, as well as makes suggestions toward the creation of a decision‐making model that can inform scene of crime officers in regards to trace selection and decisions about trace analysis. This article is categorized under:Forensic Biology > DNA Databases and BiometricsCrime Scene Investigation > From Traces to Intelligence and Evidence [ABSTRACT FROM AUTHOR]

Pregnancy‐associated atypical hemolytic uremic syndrome (P‐aHUS) is a rare disease. There are only few reports in the literature, and most are in the puerperium period. It is a thrombotic microangiopathy (TMA) characterized for microangiopathic hemolytic anemia, thrombocytopenia, and renal dysfunction. We report the case of a pregnant patient at 26.3 gestation weeks, who developed clinical features of TMA, neurological alterations, and septic shock; then after fetus and placental delivery, no clinical improvement was observed; a diagnostic protocol was performed due to suspicion of P‐aHUS, showing improvement after the plasma exchange sessions and eculizumab. We present here a brief review of the case since it is an entity that needs to be suspected during pregnancy when TMA features and requires an immediate diagnosis to provide timely treatment. [ABSTRACT FROM AUTHOR]

Modulus of elasticity of recycled concrete Recycled concrete can differ in hardened concrete properties from concrete only with natural aggregate. For this reason, when ordering recycled concrete, Switzerland requires the specification of an E‐modulus class. The modulus of elasticity must be determined in accordance with SN EN 12390‐13, i. e. on test specimens that are stored under water. Therefore, the question arose as to whether the modulus of elasticity determined on water‐saturated test specimens is meaningful for structural members with dry exposure conditions. For this reason, investigations were carried out on recycled concrete with recycled aggregates made from crushed concrete and from mixed granulate. For this purpose, test specimens were stored under water until the test was carried out and further specimens were dried before the test. It was shown that the modulus of elasticity is higher with water storage than after dry storage, but the differences are not very large. [ABSTRACT FROM AUTHOR]

Habitat degradation and fragmentation are two of the main drivers for biodiversity loss. To mitigate the negative impact of fragmentation in forests, conservation targets are increasingly addressing connectivity to facilitate the independent movement of species between habitat fragments to ensure genetic diversity and adaptation to climate change. In this article, we present a novel approach to identifying and prioritizing stepping stones for preserving connectivity based on national and regional biodiversity data for Austrian forest ecosystems. Our study identified forest areas where conservation measures should be taken to ensure future habitat connectivity by combining four indicator values with different requirements of a stepping stone habitat into a prioritization value. The four compounded indicators are: (i) the Protect Value, which includes distances to patches of protected areas with restricted management for the undisturbed development of retention areas, (ii) the Connect Value, which combines datasets of designated habitat corridors and connectivity areas in Austria based on landscape models and expert validation, (iii) the Species Value identifying species‐rich areas, and (iv) the Habitat Value identifying biotopes of high ecological value, key biodiversity areas, and sites of favorable protection status. Nonparametric tests revealed significant differences in prioritization value among the ecoregions of Austria and therefore encourage the consideration of stepping stone prioritization at local and regional context. Building upon the insights from this case study on Austrian forest ecosystems, we developed a robust framework derived from our methodology. This framework is designed to facilitate future implementations in diverse study regions, accounting for factors beyond connectivity crucial for identifying high value stepping stone habitats. We encourage adaptation of this framework to local data availability, species requirements, and local conditions. The compiled framework provides decision support for managers and conservationists for prioritizing areas to conserve and improve connectivity of forest habitats. However, it does not substitute on‐the‐ground field assessments of habitat quality and measures of functional connectivity. [ABSTRACT FROM AUTHOR]

Full electric aircraft with zero CO2 emissions during operations are a promising pathway towards achieving the Fly Net Zero by 2050 goal proposed by the International Air Transport Association (IATA). In this context, Urban Air Mobility (UAM) expects to offer an environmentally friendly alternative through electric Vertical Takeoff and Landing (eVTOL) aircraft. While eVTOLs produce no greenhouse gas emissions, the comprehensive eco‐efficiency of UAM goes beyond the flight phase. This paper delves into evaluating UAM operation's environmental and social impacts, considering the urban space, public perception, operational profiles, and power consumption. Employing causal loop analysis, the study uncovers the relationships that add value or increase impact, assessing UAM passenger transportation's eco‐efficiency. Moreover, the Unified Architecture Framework is used to model the UAM ecosystem and to propose strategies to balance values and impacts in achieving eco‐efficiency. By shedding light on the sustainability viewpoint, this study emphasizes the importance of holistically understanding UAM's operational impact and empowering users to make eco‐efficient choices when opting for UAM transportation. Finally, strategies to provide sustainability awareness to the user are discussed in the Mobility‐as‐a‐System (MaaS) context. [ABSTRACT FROM AUTHOR]

The registration of herbicides in the European Union requires an assessment of risks to nontarget terrestrial plants (NTTPs). Regulatory plant studies are performed to determine risk‐assessment‐relevant endpoints (50% effect rate) for quantitative parameters, mostly biomass and survival. Recently, the European Food Safety Authority stated that endpoints for qualitatively assessed plant visual injuries (PVIs) such as necrosis, chlorosis, and so forth should be considered for the risk assessment as equal to endpoints derived from quantitatively determined parameters. However, the lack of guidance in the NTTP test guidelines on how to assess PVI and how to derive a statistically meaningful endpoint for PVI makes their use in risk assessments challenging. To evaluate and improve the reliability, reproducibility, and regulatory relevance of PVI assessments in NTTP studies, the PVI Working Group was formed in 2022 within the SETAC Plant Interest Group. In a first exercise, research needs, guidance gaps, and shortcomings in current methodologies were identified and are presented together with recommendations for a future, validated, and harmonized method for the assessment of PVI. Survey results revealed a high variability in how PVI are currently assessed, and that the reliability of these data is unclear. Under current conditions, the PVI data can rather be seen as supportive information instead of using the data for the statistically sound determination of a regulatory endpoint. Consequently, standardization and harmonization of procedures for the assessment of PVI are needed. An improved scoring methodology should be developed that allows for a precise, statistically sound endpoint determination. Regarding the regulatory relevance of PVI, further research is required to assess the biological meaning of PVI data and how this is connected to the regulatory requirements and protection goals. Last but not least, guidance is required on how to evaluate the historically available PVI data that are based on various assessment methodologies. Integr Environ Assess Manag 2024;20:915–923. © 2023 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC). Key Points: The lack of guidance in the nontarget terrestrial plant (NTTP) OECD test guidelines on assessing plant visual injury (PVI) and deriving statistically meaningful endpoints contradicts the European Union's requirement to use PVI assessments for risk assessment of NTTPs.Under current conditions, the PVI data can rather be seen as supportive data instead of using the data for the precise determination of a regulatory endpoint.Further research is required to assess the biological meaning of PVI data and how this is connected to the regulatory requirements and protection goals.In the future, a more appropriate scoring methodology for evaluating PVI should be established, encompassing (1) a logically consistent scoring scale, (2) reproducible scores, (3) objective scoring, and (4) scores with distinct biological meaning that align with regulatory requirements. [ABSTRACT FROM AUTHOR]