1. H2B ubiquitylation modulates spliceosome assembly and function in budding yeast.
- Author
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Hérissant L, Moehle EA, Bertaccini D, Van Dorsselaer A, Schaeffer-Reiss C, Guthrie C, and Dargemont C
- Subjects
- Histones genetics, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae genetics, Spliceosomes genetics, Histones metabolism, Saccharomyces cerevisiae metabolism, Spliceosomes metabolism, Ubiquitination genetics
- Abstract
Background Information: Commitment to splicing occurs co-transcriptionally, but a major unanswered question is the extent to which various modifications of chromatin, the template for transcription in vivo, contribute to the regulation of splicing., Results: Here, we perform genome-wide analyses showing that inhibition of specific marks - H2B ubiquitylation, H3K4 methylation and H3K36 methylation - perturbs splicing in budding yeast, with each modification exerting gene-specific effects. Furthermore, semi-quantitative mass spectrometry on purified nuclear mRNPs and chromatin immunoprecipitation analysis on intron-containing genes indicated that H2B ubiquitylation, but not Set1-, Set2- or Dot1-dependent H3 methylation, stimulates recruitment of the early splicing factors, namely U1 and U2 snRNPs, onto nascent RNAs., Conclusions: These results suggest that histone modifications impact splicing of distinct subsets of genes using distinct pathways., (© 2014 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.)
- Published
- 2014
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