1. Long-term incubation with IL-4 and IL-10 oppositely modifies procoagulant activity of monocytes and modulates the surface expression of tissue factor and tissue factor pathway inhibitor.
- Author
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Paysant J, Soria C, Cornillet-Lefèbvre P, Nguyen P, Lenormand B, Mishal Z, Vannier JP, and Vasse M
- Subjects
- Antibodies, Monoclonal immunology, Cells, Cultured, Cytoplasm metabolism, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay methods, Gene Expression, Humans, Lipoproteins antagonists & inhibitors, Lipoproteins genetics, Lipoproteins metabolism, Microscopy, Confocal, Monocytes metabolism, RNA, Messenger genetics, Reverse Transcriptase Polymerase Chain Reaction methods, Thromboplastin genetics, Time Factors, Blood Coagulation Factors metabolism, Interleukin-10 pharmacology, Interleukin-4 pharmacology, Monocytes drug effects, Thromboplastin metabolism
- Abstract
Monocytes can be induced to express both tissue factor (TF) and its inhibitor, TF pathway inhibitor-1 (TFPI-1). A short incubation (<6 h) with interleukin (IL)-4 and IL-10, two potent deactivators of monocyte functions, has been shown to modulate the synthesis and expression of TF by monocytes activated by lipopolysaccharide, but the consequences of longer incubations (up to 96 h) on both TF and TFPI-1 are unknown. The results of this study showed that adherent monocytes in culture spontaneously expressed TF and TFPI and that prolonged incubation with IL-10 induced a time- and dose-dependent decrease of monocyte TF synthesis, and an accumulation of TF/TFPI-1 complexes at the moncyte surface, suggesting a decreased clearance of these complexes. In contrast, IL-4 induced a time- and dose-dependent increase in TF synthesis, which remained intracytoplasmic, as shown by confocal microscopy. Surprisingly, TF:antigen (Ag) was decreased at the monocyte surface, but the procoagulant activity (PCA) of IL-4-treated monocytes was increased, as a result of more pronounced decrease of TFPI-1:Ag expression than that of TF. In conclusion, prolonged incubation with IL-4 and IL-10 oppositely modified PCA of cultured monocytes, and altered TF and TFPI trafficking and clearance. These data explain the respective deleterious or benefit effects of IL-4 or IL-10 in atherothrombosis.
- Published
- 2005
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