1. Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy.
- Author
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Lin MH, Liu YC, Liu SY, Chen FC, Yang PJ, Li GH, Liu PY, and Yen CY
- Subjects
- Benzyl Alcohol pharmacology, Cell Line, Tumor drug effects, Down-Regulation drug effects, Humans, Hydrazones pharmacology, Microtubule-Associated Proteins metabolism, Nuts chemistry, Plant Extracts chemistry, RNA, Small Interfering metabolism, Areca chemistry, Autophagy drug effects, Clathrin pharmacology, Endocytosis drug effects, Mouth Neoplasms chemically induced, Plant Extracts pharmacology
- Abstract
Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy., Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells., Results: Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells., Conclusions: These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2018
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