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Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy.
- Source :
- Journal of Oral Pathology & Medicine; Jan2018, Vol. 47 Issue 1, p25-31, 7p, 6 Graphs
- Publication Year :
- 2018
-
Abstract
- <bold>Background: </bold>We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy.<bold>Methods: </bold>We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells.<bold>Results: </bold>Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells.<bold>Conclusions: </bold>These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09042512
- Volume :
- 47
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Oral Pathology & Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 127566566
- Full Text :
- https://doi.org/10.1111/jop.12593