1. Persistent replication of a hepatitis C virus genotype 1b-based chimeric clone carrying E1, E2 and p6 regions from GB virus B in a New World monkey
- Author
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Noboru Maki, Atsunori Higashino, Kenichi Mori, Hirofumi Akari, Yasuhiro Yasutomi, Yuki Iwasaki, and Saori Suzuki
- Subjects
0301 basic medicine ,Hepatitis B virus DNA polymerase ,Hepatitis C virus ,Immunology ,virus diseases ,Viral transformation ,Biology ,medicine.disease_cause ,Microbiology ,Virology ,digestive system diseases ,Hepatitis B virus PRE beta ,Virus ,03 medical and health sciences ,Chimera (genetics) ,030104 developmental biology ,0302 clinical medicine ,Chimeric RNA ,medicine ,030211 gastroenterology & hepatology ,Oncovirus - Abstract
The development of effective hepatitis C virus (HCV) vaccines is essential for the prevention of further HCV dissemination, especially in developing countries. Therefore the aim of this study is to establish a feasible and immunocompetent surrogate animal model of HCV infection that will help in evaluation of the protective efficacy of newly developing HCV vaccine candidates. To circumvent the narrow host range of HCV, an HCV genotype 1b-based chimeric clone carrying E1, E2 and p6 regions from GB virus B (GBV-B), which is closely related to HCV, was generated. The chimera between HCV and GBV-B, named HCV/G, replicated more efficiently as compared with the HCV clone in primary marmoset hepatocytes. Furthermore, it was found that the chimera persistently replicated in a tamarin for more than 2 years after intrahepatic inoculation of the chimeric RNA. Although relatively low (
- Published
- 2016