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Persistent replication of a hepatitis C virus genotype 1b-based chimeric clone carrying E1, E2 and p6 regions from GB virus B in a New World monkey
- Source :
- Microbiology and Immunology. 60:26-34
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- The development of effective hepatitis C virus (HCV) vaccines is essential for the prevention of further HCV dissemination, especially in developing countries. Therefore the aim of this study is to establish a feasible and immunocompetent surrogate animal model of HCV infection that will help in evaluation of the protective efficacy of newly developing HCV vaccine candidates. To circumvent the narrow host range of HCV, an HCV genotype 1b-based chimeric clone carrying E1, E2 and p6 regions from GB virus B (GBV-B), which is closely related to HCV, was generated. The chimera between HCV and GBV-B, named HCV/G, replicated more efficiently as compared with the HCV clone in primary marmoset hepatocytes. Furthermore, it was found that the chimera persistently replicated in a tamarin for more than 2 years after intrahepatic inoculation of the chimeric RNA. Although relatively low (
- Subjects :
- 0301 basic medicine
Hepatitis B virus DNA polymerase
Hepatitis C virus
Immunology
virus diseases
Viral transformation
Biology
medicine.disease_cause
Microbiology
Virology
digestive system diseases
Hepatitis B virus PRE beta
Virus
03 medical and health sciences
Chimera (genetics)
030104 developmental biology
0302 clinical medicine
Chimeric RNA
medicine
030211 gastroenterology & hepatology
Oncovirus
Subjects
Details
- ISSN :
- 03855600
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Microbiology and Immunology
- Accession number :
- edsair.doi...........7a267a8bfd912d5a102e590cc48fd5d5