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2. In situ measurement of 3D contact angle in sand based on X‐ray computed tomography

Author

Steffen Schlüter, Sebastian R. G. A. Blaser, Pascal Benard, and Andrea Carminati

Subjects
Environmental sciences, GE1-350, Geology, QE1-996.5
Abstract

Abstract Soil water repellency is traditionally expressed as contact angle (CA) and measured destructively on exposed surfaces or derived from flow behavior or measured forces. These approaches cannot map local heterogeneities in CA, which typically exist in intact soil. Here, we explore the potential and limitations of in situ measurements of three‐dimensional CAs with simplified benchmark tests and in intact sand. The method is based on a protocol for the freely available OpenFOAM software and applied to segmented X‐ray computed tomography (X‐ray CT) data. This study scrutinizes its suitability under typical vadose zone conditions, as it was originally developed for rock studies in petroleum engineering. The assets of the method are that it allows for accurate measurement of locally varying, equilibrium contact angles and that they can be linked to pore scale features that cause them. This is demonstrated with rehydrated mucilage structures in sand and the associated change in local CAs, where they touch sand grains. In general, the image acquisition time of polychromatic X‐ray CT (minutes) precludes the assessments of dynamic CAs that may equilibrate within seconds. Another limitation is that acute CAs are overestimated due to the voxel discretization of interfaces and contact lines in combination with image smoothing. The divergence arises around 60° and is most severe in the limit of vanishing water repellency. In summary, the method enables the mapping of local heterogeneities of equilibrium CAs, though the absolute values should be critically assessed.

Published
2022
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4. Age‐related telomere attrition in the human putamen

Author

Schreglmann, Sebastian R., primary, Goncalves, Tomas, additional, Grant‐Peters, Melissa, additional, Kia, Demis A., additional, Soreq, Lilach, additional, Ryten, Mina, additional, Wood, Nicholas W., additional, Bhatia, Kailash P., additional, and Tomita, Kazunori, additional

Published
2023
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7. Dermal Real‐Time Quaking‐Induced Conversion Is a Sensitive Marker to Confirm Isolated Rapid Eye Movement Sleep Behavior Disorder as an Early α‐Synucleinopathy

Author

Kuzkina, Anastasia, primary, Panzer, Celine, additional, Seger, Aline, additional, Schmitt, Daniela, additional, Rößle, Jonas, additional, Schreglmann, Sebastian R., additional, Knacke, Henrike, additional, Salabasidou, Elena, additional, Kohl, Antonia, additional, Sittig, Elisabeth, additional, Barbe, Michael, additional, Berg, Daniela, additional, Volkmann, Jens, additional, Sommer, Claudia, additional, Oertel, Wolfgang H., additional, Schaeffer, Eva, additional, Sommerauer, Michael, additional, Janzen, Annette, additional, and Doppler, Kathrin, additional

Published
2023
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8. Outcomes in emergency versus electively scheduled cases of placenta accreta spectrum disorder managed by cesarean‐hysterectomy within a multidisciplinary care team

Author

Homero Flores‐Mendoza, Anjana Ravi Chandran, Carlos Hernandez‐Nieto, Ally Murji, Lisa Allen, Rory C. Windrim, John C. Kingdom, and Sebastian R. Hobson

Subjects
Patient Care Team, Cesarean Section, Pregnancy, Infant, Newborn, Humans, Obstetrics and Gynecology, Female, Placenta Accreta, General Medicine, Hysterectomy, Retrospective Studies
Abstract

Compare maternal and perinatal outcomes between emergency and electively scheduled cesarean-hysterectomy for placenta accreta spectrum (PAS) disorders.Single-center retrospective cohort study including 125 cases of antenatally suspected and pathologically confirmed PAS disorders. Maternal and perinatal outcomes were analyzed. Multivariate logistic regression was used to test associations. Survival curves exploring risk factors for emergency birth were sought.25 (20%) and 100 (80%) patients had emergency and electively scheduled birth, respectively. Emergency birth had a higher estimated blood loss (2772 [2256.75] vs. 1561.19 [1152.95], P 0.001), with a higher rate of coagulopathy (40% vs. 6%; P 0.001) and bladder injury (44% vs. 13%; P 0.001); and was associated with increased rates of blood transfusion (aOR 4.9, CI95% 1.3-17.5, P = 0.01), coagulopathy (aOR 16.4, CI95% 2.6-101.4, P = 0.002) and urinary tract injury (aOR 6.96, CI95% 1.5-30.7, P = 0.01). Gestational age at birth was lower in the emergency group (31.55 [4.75] vs. 35.19 [2.77], P = 0.001), no difference in perinatal mortality was found. A sonographically short cervix and/or history of APH had an increased cumulative risk of emergency birth with advancing gestational age.Patients with PAS disorders managed in a tertiary center by a multidisciplinary team requiring emergency birth have increased maternal morbidity and poorer perinatal outcomes than those with electively scheduled birth.

Published
2022
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11. Dermal Real‐Time Quaking‐Induced Conversion Is a Sensitive Marker to Confirm Isolated Rapid Eye Movement Sleep Behavior Disorder as an Early α‐Synucleinopathy

Author

Anastasia Kuzkina, Celine Panzer, Aline Seger, Daniela Schmitt, Jonas Rößle, Sebastian R. Schreglmann, Henrike Knacke, Elena Salabasidou, Antonia Kohl, Elisabeth Sittig, Michael Barbe, Daniela Berg, Jens Volkmann, Claudia Sommer, Wolfgang H. Oertel, Eva Schaeffer, Michael Sommerauer, Annette Janzen, and Kathrin Doppler

Subjects
Neurology, Neurology (clinical)
Published
2023
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13. A surrogate cell‐based SARS‐CoV‐2 spike blocking assay

Author

Sebastian R. Schulz, Hans-Martin Jäck, Manfred Rauh, Tobit Steinmetz, Michael Reth, Manuela Hauke, Lena Baus, Sara Krause, Holm Schneider, Wolfgang Schuh, Patrick Morhart, Dirk Mielenz, Edith Roth, Leonie Weckwerth, Niklas Vesper, and Markus Hoffmann

Subjects
viruses, Immunology, Spike protein, Biology, Antibodies, Viral, SARS‐CoV‐2, COVID-19 Serological Testing, law.invention, 03 medical and health sciences, COVID‐19, law, Blocking antibody, Humans, Immunology and Allergy, ddc:610, Basic, Antibodies, Blocking, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, SARS-CoV-2, 030306 microbiology, Blocking (radio), fungi, Antibody titer, COVID-19, hACE2, virus diseases, Flow Cytometry, Antibodies, Neutralizing, Virology, New Technology, 3. Good health, Vaccination, Enzyme, chemistry, Spike Glycoprotein, Coronavirus, Recombinant DNA, biology.protein, Research Article|Basic, Spike (software development), Antibody, Surrogate blocking assay, Research Article
Abstract

To monitor infection by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and successful vaccination against coronavirus disease 2019 (COVID‐19), the kinetics of neutralizing or blocking anti‐SARS‐CoV‐2 antibody titers need to be assessed. Here, we report the development of a quick and inexpensive surrogate SARS‐CoV‐2 blocking assay (SUBA) using immobilized recombinant human angiotensin‐converting enzyme 2 (hACE2) and human cells expressing the native form of surface SARS‐CoV‐2 spike protein. Spike protein‐expressing cells bound to hACE2 in the absence or presence of blocking antibodies were quantified by measuring the optical density of cell‐associated crystal violet in a spectrophotometer. The advantages are that SUBA is a fast and inexpensive assay, which does not require biosafety level 2‐ or 3‐approved laboratories. Most importantly, SUBA detects blocking antibodies against the native trimeric cell‐bound SARS‐CoV‐2 spike protein and can be rapidly adjusted to quickly pre‐screen already approved therapeutic antibodies or sera from vaccinated individuals for their ACE2 blocking activities against any emerging SARS‐CoV‐2 variants., A simple, inexpensive surrogate SARS‐CoV‐2 neutralization assay is introduced. Ectopic expression of the SARS‐CoV‐2 spike protein confers binding of Ramos‐Ig ‐ or HEK293 cells to plate‐bound recombinant human angiotensin‐converting‐enzyme‐2. This interaction can be blocked with reconvalescent or immune serum or with anti‐receptor‐binding domain antibodies. Bound spike‐expressing cells are quantitated with crystal violet.

Published
2021
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14. The 3‐D Imaging of Roots Growing in Soil

Author

Sebastian R. G. A. Blaser, Steffen Schlüter, Doris Vetterlein, and Nicolai Koebernick

Subjects
Root growth, Noninvasive imaging, Soil water, Soil science, Root hair, Biology, 3 d imaging, Soil compaction (agriculture)
Published
2021
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15. Placenta Accreta Spectrum 2021: Roundtable Discussion

Author

Vineet K. Shrivastava, Brett D. Einerson, Karin A. Fox, Wesley Lee, Sally Collins, Sebastian R. Hobson, José M Palacios-Jaraquemada, and Christopher I. Cassady

Subjects
medicine.medical_specialty, Radiological and Ultrasound Technology, Cesarean Section, Obstetrics, business.industry, Placenta accreta, Placenta, Placenta Previa, MEDLINE, Placenta Accreta, Hysterectomy, medicine.disease, Pregnancy, medicine, Humans, Female, Radiology, Nuclear Medicine and imaging, business, Retrospective Studies
Published
2021
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16. Determination of fetal heart rate short‐term variation from umbilical artery Doppler waveforms

Author

John Kingdom, Greg Stortz, Sebastian R. Hobson, Natasha Milligan, Clare Whitehead, Lindsay S. Cahill, Shiri Shinar, Christopher K. Macgowan, John G. Sled, S. Millard, Ahmet Baschat, and A. Ravi Chandran

Subjects
Adult, Male, Middle Cerebral Artery, medicine.medical_specialty, Cardiotocography, Gestational Age, Ultrasonography, Prenatal, Umbilical Arteries, Article, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Pregnancy, Interquartile range, medicine.artery, Internal medicine, Heart rate, medicine, Humans, Radiology, Nuclear Medicine and imaging, Longitudinal Studies, Prospective Studies, 030212 general & internal medicine, Fetal Growth Retardation, 030219 obstetrics & reproductive medicine, Radiological and Ultrasound Technology, medicine.diagnostic_test, business.industry, Ultrasound, Infant, Newborn, Obstetrics and Gynecology, Gestational age, Ultrasonography, Doppler, Umbilical artery, General Medicine, Heart Rate, Fetal, Reproductive Medicine, Cardiology, symbols, Gestation, Female, business, Doppler effect
Abstract

OBJECTIVES: To evaluate the feasibility of using umbilical artery (UA) Doppler waveforms to measure fetal heart rate short term variation (STV) across gestation. METHODS: A prospective longitudinal study was conducted at two study sites in 195 women with pregnancies considered low risk. Pulsed wave Doppler of the UAs was performed at a 4-weekly interval between 14–40 weeks of gestation using a standardized imaging protocol. Up to 12 consecutive UA Doppler waveforms were analyzed using an off-line processing software. Fetal heart rate STV was calculated using average R-to-R intervals extracted from the waveforms and baseline corrected for fetal heart rate. RESULTS: Baseline corrected short term fetal heart rate variation increased significantly with gestational age (conditional R(2) = 0.37, p < 0.0001) and was inversely correlated with fetal heart rate (conditional R(2) = 0.54, p < 0.0001). The short term variation ranged (median (interquartile range)) from 3.5 (2.9–4.1) ms at 14–20 weeks’ gestation to 6.3 (4.8–7.7) ms at 34–40 weeks’ gestation. The change in short term heart rate variation did not differ between study sites, different models of ultrasound machine or individual sonographers. CONCLUSIONS: Umbilical artery Doppler waveforms offer a robust and feasible method to derive short term variation of the fetal heart rate. It needs to be emphasized that the umbilical-Doppler derived short term variation is not interchangeable with measurements derived with computerized cardiotocography. Accordingly, further investigations need to validate associations with outcome to determine the value of concurrent fetal cardiovascular and heart rate evaluation that are possible with the technique described here.

Published
2020
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19. Augmented neutralization of SARS‐CoV‐2 Omicron variant by boost vaccination and monoclonal antibodies

Author

Schulz, Sebastian R., primary, Hoffmann, Markus, additional, Roth, Edith, additional, Pracht, Katharina, additional, Burnett, Deborah L., additional, Mazigi, Ohan, additional, Schuh, Wolfgang, additional, Manger, Bernhard, additional, Mielenz, Dirk, additional, Goodnow, Christopher C., additional, Christ, Daniel, additional, Pöhlmann, Stefan, additional, and Jäck, Hans‐Martin, additional

Published
2022
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24. Modeling Metastatic Colonization in a Decellularized Organ Scaffold‐Based Perfusion Bioreactor (Adv. Healthcare Mater. 1/2022)

Author

Rafaeva, Maria, primary, Horton, Edward R., additional, Jensen, Adina R.D., additional, Madsen, Chris D., additional, Reuten, Raphael, additional, Willacy, Oliver, additional, Brøchner, Christian B., additional, Jensen, Thomas H., additional, Zornhagen, Kamilla Westarp, additional, Crespo, Marina, additional, Grønseth, Dina S., additional, Nielsen, Sebastian R., additional, Idorn, Manja, additional, Straten, Per thor, additional, Rohrberg, Kristoffer, additional, Spanggaard, Iben, additional, Højgaard, Martin, additional, Lassen, Ulrik, additional, Erler, Janine T., additional, and Mayorca‐Guiliani, Alejandro E., additional

Published
2022
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25. Modeling Metastatic Colonization in a Decellularized Organ Scaffold‐Based Perfusion Bioreactor

Author

Rafaeva, Maria, primary, Horton, Edward R., additional, Jensen, Adina R.D., additional, Madsen, Chris D., additional, Reuten, Raphael, additional, Willacy, Oliver, additional, Brøchner, Christian B., additional, Jensen, Thomas H., additional, Zornhagen, Kamilla Westarp, additional, Crespo, Marina, additional, Grønseth, Dina S., additional, Nielsen, Sebastian R., additional, Idorn, Manja, additional, Straten, Per thor, additional, Rohrberg, Kristoffer, additional, Spanggaard, Iben, additional, Højgaard, Martin, additional, Lassen, Ulrik, additional, Erler, Janine T., additional, and Mayorca‐Guiliani, Alejandro E., additional

Published
2021
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26. Author response for 'A pair of non‐competing neutralizing human monoclonal antibodies protecting from disease in a SARS‐CoV‐2 infection model'

Author

Roman Wölfel, Paul F. McKay, Jutta Eichler, Stefan Pöhlmann, Manuela Hauke, Dirk Mielenz, Edith Roth, Eva Grüner, Matthias Tenbusch, Thomas Grunwald, Thomas Winkler, Tobit Steinmetz, Sebastian R. Schulz, Ralf Wagner, Heinrich Sticht, Markus Hoffmann, Hans-Martin Jäck, Sandra Ciesek, Dominik Damm, Valentina Eberlein, Eileen Socher, Leila Issmail, Katharina Habenicht, Armin Ensser, David Peterhoff, Elie Richel, Wolfgang Schuh, Klaus Überla, Nadja Uhlig, Antonia Sophia Peter, Simon Dolles, Frank Neipel, Sandra Mueller-Schmucker, Robin J. Shattock, and Kirsten Fraedrich

Subjects
medicine.drug_class, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), medicine, Disease, Biology, Monoclonal antibody, Virology
Published
2021
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27. A surrogate cell‐based SARS‐CoV‐2 spike blocking assay (SUBA)

Author

Schuh, Wolfgang, primary, Baus, Lena, additional, Steinmetz, Tobit, additional, Schulz, Sebastian R., additional, Weckwerth, Leonie, additional, Roth, Edith, additional, Hauke, Manuela, additional, Krause, Sara, additional, Morhart, Patrick, additional, Rauh, Manfred, additional, Hoffmann, Markus, additional, Vesper, Niklas, additional, Reth, Michael, additional, Schneider, Holm, additional, Jäck, Hans‐Martin, additional, and Mielenz, Dirk, additional

Published
2021
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28. A pair of noncompeting neutralizing human monoclonal antibodies protecting from disease in a SARS‐CoV‐2 infection model

Author

Peter, Antonia Sophia, primary, Roth, Edith, additional, Schulz, Sebastian R., additional, Fraedrich, Kirsten, additional, Steinmetz, Tobit, additional, Damm, Dominik, additional, Hauke, Manuela, additional, Richel, Elie, additional, Mueller‐Schmucker, Sandra, additional, Habenicht, Katharina, additional, Eberlein, Valentina, additional, Issmail, Leila, additional, Uhlig, Nadja, additional, Dolles, Simon, additional, Grüner, Eva, additional, Peterhoff, David, additional, Ciesek, Sandra, additional, Hoffmann, Markus, additional, Pöhlmann, Stefan, additional, McKay, Paul F., additional, Shattock, Robin J., additional, Wölfel, Roman, additional, Socher, Eileen, additional, Wagner, Ralf, additional, Eichler, Jutta, additional, Sticht, Heinrich, additional, Schuh, Wolfgang, additional, Neipel, Frank, additional, Ensser, Armin, additional, Mielenz, Dirk, additional, Tenbusch, Matthias, additional, Winkler, Thomas H., additional, Grunwald, Thomas, additional, Überla, Klaus, additional, and Jäck, Hans‐Martin, additional

Published
2021
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30. OC02.02: *Screening for placenta accreta spectrum in Canada in early pregnancy: analysis of retrospectively deemed high‐risk women candidates for screening

Author

John Kingdom, Sebastian R. Hobson, Rory Windrim, and H. Flores Mendoza

Subjects
medicine.medical_specialty, Radiological and Ultrasound Technology, biology, Placenta accreta, business.industry, Obstetrics, Obstetrics and Gynecology, Early pregnancy factor, General Medicine, medicine.disease, Reproductive Medicine, medicine, biology.protein, Radiology, Nuclear Medicine and imaging, business
Published
2021
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31. Doppler Ultrasound of the Fetal Descending Aorta: An Objective Tool to Assess Placental Blood Flow Resistance in Pregnancies With Discordant Umbilical Arteries

Author

Cahill, Lindsay S., primary, Mercer, Grace V., additional, Jagota, Dakshita, additional, Ravi Chandran, Anjana, additional, Milligan, Natasha, additional, Shinar, Shiri, additional, Whitehead, Clare L., additional, Hobson, Sebastian R., additional, Serghides, Lena, additional, Parks, W. Tony, additional, Macgowan, Christopher K., additional, Kingdom, John C., additional, Sled, John G., additional, and Baschat, Ahmet A., additional

Published
2021
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32. Safer outcomes for placenta accreta spectrum disorders: A decade of quality improvement

Author

Hobson, Sebastian R., primary, Kingdom, John C. P., additional, Windrim, Rory C., additional, Murji, Ally, additional, Milligan, Natasha, additional, Pacheco, Jessica F., additional, Lu, Catherine, additional, Steckham, Katherine E., additional, Kajal, Dilkash, additional, Pantazi, Sophia, additional, Carvalho, Jose C. A., additional, Parks, W. Tony, additional, and Allen, Lisa M., additional

Published
2021
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34. Seedball‐induced changes of root growth and physico‐chemical properties—a case study with pearl millet

Author

Ludger Herrmann, Doris Vetterlein, Sebastian R. G. A. Blaser, Günter Neumann, and Charles Ikenna Nwankwo

Subjects
0106 biological sciences, Root growth, Soil Science, 04 agricultural and veterinary sciences, Plant Science, Biology, engineering.material, 01 natural sciences, Horticulture, 040103 agronomy & agriculture, engineering, 0401 agriculture, forestry, and fisheries, Pearl, 010606 plant biology & botany
Published
2018
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35. Placenta Accreta Spectrum 2021: Roundtable Discussion

Author

Fox, Karin A., primary, Cassady, Christopher I., additional, Collins, Sally, additional, Einerson, Brett D., additional, Hobson, Sebastian R., additional, Palacios‐Jaraquemada, José M., additional, Shrivastava, Vineet K., additional, and Lee, Wesley, additional

Published
2021
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37. miR‐148a controls metabolic programming and survival of mature CD19‐negative plasma cells in mice

Author

Pracht, Katharina, primary, Meinzinger, Julia, additional, Schulz, Sebastian R., additional, Daum, Patrick, additional, Côrte‐Real, Joana, additional, Hauke, Manuela, additional, Roth, Edith, additional, Kindermann, Dorothea, additional, Mielenz, Dirk, additional, Schuh, Wolfgang, additional, Wittmann, Jürgen, additional, and Jäck, Hans‐Martin, additional

Published
2021
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38. Modeling Metastatic Colonization in a Decellularized Organ Scaffold‐Based Perfusion Bioreactor

Author

Dina S. Grønseth, Raphael Reuten, Ulrik Lassen, Martin Højgaard, Kamilla Westarp Zornhagen, Manja Idorn, Chris D. Madsen, Marina Crespo, Kristoffer Staal Rohrberg, Christian B. Brøchner, Maria Rafaeva, Janine T. Erler, Adina R. D. Jensen, Alejandro E Mayorca-Guiliani, Oliver Willacy, Per thor Straten, Thomas Hartvig Lindkær Jensen, Edward R. Horton, Sebastian R. Nielsen, and Iben Spanggaard

Subjects
Cell type, Cell signaling, experimental methods, specialized bioreactors, extracellular matrix, Cell, Biomedical Engineering, Pharmaceutical Science, Context (language use), Biology, Biomaterials, Extracellular matrix, Mice, 03 medical and health sciences, Bioreactors, 0302 clinical medicine, cancer metastasis, medicine, Animals, 030304 developmental biology, 0303 health sciences, Tumor microenvironment, Decellularization, Tissue Engineering, Tissue Scaffolds, Extracellular Matrix, 3. Good health, Cell biology, Perfusion, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Cancer cell, Proteoglycans
Abstract

Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.

Published
2021
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40. Movement disorders in genetically confirmed mitochondrial disease and the putative role of the cerebellum

Author

Marian Galovic, Georg Kägi, André Schaller, Christos Ganos, Zane Jaunmuktane, Ernst Krasemann, Franz Riederer, Sebastian R Schreglmann, Lars Michels, Daniel Waldvogel, Christian R. Baumann, Ute Hidding, Kailash P. Bhatia, and Hans H. Jung

Subjects
0301 basic medicine, Dystonia, Pathology, medicine.medical_specialty, Movement disorders, Ataxia, Mitochondrial disease, Chorea, Biology, medicine.disease, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Neurology, Hypokinesia, medicine, Neurology (clinical), medicine.symptom, Hyperkinesia, Myoclonus, 030217 neurology & neurosurgery
Abstract

Background: Mitochondrial disease can present as a movement disorder. Data on this entity's epidemiology, genetics, and underlying pathophysiology, however, is scarce. Objective: The objective of this study was to describe the clinical, genetic, and volumetric imaging data from patients with mitochondrial disease who presented with movement disorders. Methods: In this retrospective analysis of all genetically confirmed mitochondrial disease cases from three centers (n = 50), the prevalence and clinical presentation of video-documented movement disorders was assessed. Voxel-based morphometry from high-resolution MRI was employed to compare cerebral and cerebellar gray matter volume between mitochondrial disease patients with and without movement disorders and healthy controls. Results: Of the 50 (30%) patients with genetically confirmed mitochondrial disease, 15 presented with hypokinesia (parkinsonism 3/15), hyperkinesia (dystonia 5/15, myoclonus 3/15, chorea 2/15), and ataxia (3/15). In 3 patients, mitochondrial disease presented as adult-onset isolated dystonia. In comparison to healthy controls and mitochondrial disease patients without movement disorders, patients with hypo- and hyperkinetic movement disorders had significantly more cerebellar atrophy and an atrophy pattern predominantly involving cerebellar lobules VI and VII. Conclusion: This series provides clinical, genetic, volumetric imaging, and histologic data that indicate major involvement of the cerebellum in mitochondrial disease when it presents with hyper- and hypokinetic movement disorders. As a working hypothesis addressing the particular vulnerability of the cerebellum to energy deficiency, this adds substantially to the pathophysiological understanding of movement disorders in mitochondrial disease. Furthermore, it provides evidence that mitochondrial disease can present as adult-onset isolated dystonia. © 2017 International Parkinson and Movement Disorder Society

Published
2017
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41. Embedding assessment in a simulation skills training program for medical and midwifery students: A pre- and post-intervention evaluation

Author

Luke Larmour, Euan M. Wallace, Philip DeKoninck, Sebastian R. Hobson, Christine East, Helen Hall, Shavi Fernando, Arunaz Kumar, Debra Nestel, Irene Tatjana Lichtwark, Margaret Hay, Deidre Bentley, and Gayle McLelland

Subjects
Students, Health Occupations, medicine.medical_specialty, Students, Medical, 020205 medical informatics, education, 02 engineering and technology, Midwifery, Learning experience, 03 medical and health sciences, Skills training, 0302 clinical medicine, Nursing, Obstetrics and gynaecology, 0202 electrical engineering, electronic engineering, information engineering, medicine, Humans, 030212 general & internal medicine, Student learning, Intervention evaluation, Simulation Training, Pre and post, Medical education, Obstetrics, business.industry, Australia, Obstetrics and Gynecology, General Medicine, Test (assessment), Gynecology, Female, Educational Measurement, Gynecological Examination, business, Education, Medical, Undergraduate
Abstract

BACKGROUND Simulation-based programs are increasingly being used to teach obstetrics and gynaecology examinations, but it is difficult to establish student learning acquired through them. Assessment may test student learning but its role in learning itself is rarely recognised. We undertook this study to assess medical and midwifery student learning through a simulation program using a pre-test and post-test design and also to evaluate use of assessment as a method of learning. METHODS The interprofessional simulation education program consisted of a brief pre-reading document, a lecture, a video demonstration and a hands-on workshop. Over a 24-month period, 405 medical and 104 midwifery students participated in the study and were assessed before and after the program. Numerical data were analysed using paired t-test and one-way analysis of variance. Students' perceptions of the role of assessment in learning were qualitatively analysed. RESULTS The post-test scores were significantly higher than the pre-test (P

Published
2017
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42. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author

Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Acs, Andreas, additional, Adam, Dieter, additional, Adam‐Klages, Sabine, additional, Agace, William W., additional, Aghaeepour, Nima, additional, Akdis, Mübeccel, additional, Allez, Matthieu, additional, Almeida, Larissa Nogueira, additional, Alvisi, Giorgia, additional, Anderson, Graham, additional, Andrä, Immanuel, additional, Annunziato, Francesco, additional, Anselmo, Achille, additional, Bacher, Petra, additional, Baldari, Cosima T., additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Battistini, Luca, additional, Bauer, Wolfgang, additional, Baumgart, Sabine, additional, Baumgarth, Nicole, additional, Baumjohann, Dirk, additional, Baying, Bianka, additional, Bebawy, Mary, additional, Becher, Burkhard, additional, Beisker, Wolfgang, additional, Benes, Vladimir, additional, Beyaert, Rudi, additional, Blanco, Alfonso, additional, Boardman, Dominic A., additional, Bogdan, Christian, additional, Borger, Jessica G., additional, Borsellino, Giovanna, additional, Boulais, Philip E., additional, Bradford, Jolene A., additional, Brenner, Dirk, additional, Brinkman, Ryan R., additional, Brooks, Anna E. S., additional, Busch, Dirk H., additional, Büscher, Martin, additional, Bushnell, Timothy P., additional, Calzetti, Federica, additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cao, Xuetao, additional, Cardell, Susanna L., additional, Casola, Stefano, additional, Cassatella, Marco A., additional, Cavani, Andrea, additional, Celada, Antonio, additional, Chatenoud, Lucienne, additional, Chattopadhyay, Pratip K., additional, Chow, Sue, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Clerici, Mario, additional, Colombo, Federico S., additional, Cook, Laura, additional, Cooke, Anne, additional, Cooper, Andrea M., additional, Corbett, Alexandra J., additional, Cosma, Antonio, additional, Cosmi, Lorenzo, additional, Coulie, Pierre G., additional, Cumano, Ana, additional, Cvetkovic, Ljiljana, additional, Dang, Van Duc, additional, Dang‐Heine, Chantip, additional, Davey, Martin S., additional, Davies, Derek, additional, De Biasi, Sara, additional, Del Zotto, Genny, additional, Dela Cruz, Gelo Victoriano, additional, Delacher, Michael, additional, Della Bella, Silvia, additional, Dellabona, Paolo, additional, Deniz, Günnur, additional, Dessing, Mark, additional, Di Santo, James P., additional, Diefenbach, Andreas, additional, Dieli, Francesco, additional, Dolf, Andreas, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dudziak, Diana, additional, Dustin, Michael, additional, Dutertre, Charles‐Antoine, additional, Ebner, Friederike, additional, Eckle, Sidonia B. G., additional, Edinger, Matthias, additional, Eede, Pascale, additional, Ehrhardt, Götz R.A., additional, Eich, Marcus, additional, Engel, Pablo, additional, Engelhardt, Britta, additional, Erdei, Anna, additional, Esser, Charlotte, additional, Everts, Bart, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Fehniger, Todd A., additional, Felipo‐Benavent, Mar, additional, Ferry, Helen, additional, Feuerer, Markus, additional, Filby, Andrew, additional, Filkor, Kata, additional, Fillatreau, Simon, additional, Follo, Marie, additional, Förster, Irmgard, additional, Foster, John, additional, Foulds, Gemma A., additional, Frehse, Britta, additional, Frenette, Paul S., additional, Frischbutter, Stefan, additional, Fritzsche, Wolfgang, additional, Galbraith, David W., additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Gaudilliere, Brice, additional, Gazzinelli, Ricardo T., additional, Geginat, Jens, additional, Gerner, Wilhelm, additional, Gherardin, Nicholas A., additional, Ghoreschi, Kamran, additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Goda, Keisuke, additional, Godfrey, Dale I., additional, Goettlinger, Christoph, additional, González‐Navajas, Jose M., additional, Goodyear, Carl S., additional, Gori, Andrea, additional, Grogan, Jane L., additional, Grummitt, Daryl, additional, Grützkau, Andreas, additional, Haftmann, Claudia, additional, Hahn, Jonas, additional, Hammad, Hamida, additional, Hämmerling, Günter, additional, Hansmann, Leo, additional, Hansson, Goran, additional, Harpur, Christopher M., additional, Hartmann, Susanne, additional, Hauser, Andrea, additional, Hauser, Anja E., additional, Haviland, David L., additional, Hedley, David, additional, Hernández, Daniela C., additional, Herrera, Guadalupe, additional, Herrmann, Martin, additional, Hess, Christoph, additional, Höfer, Thomas, additional, Hoffmann, Petra, additional, Hogquist, Kristin, additional, Holland, Tristan, additional, Höllt, Thomas, additional, Holmdahl, Rikard, additional, Hombrink, Pleun, additional, Houston, Jessica P., additional, Hoyer, Bimba F., additional, Huang, Bo, additional, Huang, Fang‐Ping, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hunter, Christopher A., additional, Hwang, William Y. K., additional, Iannone, Anna, additional, Ingelfinger, Florian, additional, Ivison, Sabine M, additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Jávega, Beatriz, additional, Jonjic, Stipan, additional, Kaiser, Toralf, additional, Kalina, Tomas, additional, Kamradt, Thomas, additional, Kaufmann, Stefan H. E., additional, Keller, Baerbel, additional, Ketelaars, Steven L. C., additional, Khalilnezhad, Ahad, additional, Khan, Srijit, additional, Kisielow, Jan, additional, Klenerman, Paul, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kolls, Jay K., additional, Kong, Wan Ting, additional, Kopf, Manfred, additional, Korn, Thomas, additional, Kriegsmann, Katharina, additional, Kristyanto, Hendy, additional, Kroneis, Thomas, additional, Krueger, Andreas, additional, Kühne, Jenny, additional, Kukat, Christian, additional, Kunkel, Désirée, additional, Kunze‐Schumacher, Heike, additional, Kurosaki, Tomohiro, additional, Kurts, Christian, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Landry, Jonathan, additional, Lantz, Olivier, additional, Lanuti, Paola, additional, LaRosa, Francesca, additional, Lehuen, Agnès, additional, LeibundGut‐Landmann, Salomé, additional, Leipold, Michael D., additional, Leung, Leslie Y.T., additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Litwin, Virginia, additional, Liu, Yanling, additional, Ljunggren, Hans‐Gustaf, additional, Lohoff, Michael, additional, Lombardi, Giovanna, additional, Lopez, Lilly, additional, López‐Botet, Miguel, additional, Lovett‐Racke, Amy E., additional, Lubberts, Erik, additional, Luche, Herve, additional, Ludewig, Burkhard, additional, Lugli, Enrico, additional, Lunemann, Sebastian, additional, Maecker, Holden T., additional, Maggi, Laura, additional, Maguire, Orla, additional, Mair, Florian, additional, Mair, Kerstin H., additional, Mantovani, Alberto, additional, Manz, Rudolf A., additional, Marshall, Aaron J., additional, Martínez‐Romero, Alicia, additional, Martrus, Glòria, additional, Marventano, Ivana, additional, Maslinski, Wlodzimierz, additional, Matarese, Giuseppe, additional, Mattioli, Anna Vittoria, additional, Maueröder, Christian, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, McGrath, Mairi, additional, McGuire, Helen M., additional, McInnes, Iain B., additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Miller, Stephen D., additional, Mills, Kingston H.G., additional, Minderman, Hans, additional, Mjösberg, Jenny, additional, Moore, Jonni, additional, Moran, Barry, additional, Moretta, Lorenzo, additional, Mosmann, Tim R., additional, Müller, Susann, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Münz, Christian, additional, Nakayama, Toshinori, additional, Nasi, Milena, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Nourshargh, Sussan, additional, Núñez, Gabriel, additional, O'Connor, José‐Enrique, additional, Ochel, Aaron, additional, Oja, Anna, additional, Ordonez, Diana, additional, Orfao, Alberto, additional, Orlowski‐Oliver, Eva, additional, Ouyang, Wenjun, additional, Oxenius, Annette, additional, Palankar, Raghavendra, additional, Panse, Isabel, additional, Pattanapanyasat, Kovit, additional, Paulsen, Malte, additional, Pavlinic, Dinko, additional, Penter, Livius, additional, Peterson, Pärt, additional, Peth, Christian, additional, Petriz, Jordi, additional, Piancone, Federica, additional, Pickl, Winfried F., additional, Piconese, Silvia, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Podolska, Malgorzata Justyna, additional, Poon, Zhiyong, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pucillo, Carlo E. M., additional, Quataert, Sally A., additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Radstake, Tim R. D. J., additional, Rahmig, Susann, additional, Rahn, Hans‐Peter, additional, Rajwa, Bartek, additional, Ravichandran, Gevitha, additional, Raz, Yotam, additional, Rebhahn, Jonathan A., additional, Recktenwald, Diether, additional, Reimer, Dorothea, additional, Reis e Sousa, Caetano, additional, Remmerswaal, Ester B.M., additional, Richter, Lisa, additional, Rico, Laura G., additional, Riddell, Andy, additional, Rieger, Aja M., additional, Robinson, J. Paul, additional, Romagnani, Chiara, additional, Rubartelli, Anna, additional, Ruland, Jürgen, additional, Saalmüller, Armin, additional, Saeys, Yvan, additional, Saito, Takashi, additional, Sakaguchi, Shimon, additional, Sala‐de‐Oyanguren, Francisco, additional, Samstag, Yvonne, additional, Sanderson, Sharon, additional, Sandrock, Inga, additional, Santoni, Angela, additional, Sanz, Ramon Bellmàs, additional, Saresella, Marina, additional, Sautes‐Fridman, Catherine, additional, Sawitzki, Birgit, additional, Schadt, Linda, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schimisky, Esther, additional, Schlitzer, Andreas, additional, Schlosser, Josephine, additional, Schmid, Stephan, additional, Schmitt, Steffen, additional, Schober, Kilian, additional, Schraivogel, Daniel, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulte, Reiner, additional, Schulz, Axel Ronald, additional, Schulz, Sebastian R., additional, Scottá, Cristiano, additional, Scott‐Algara, Daniel, additional, Sester, David P., additional, Shankey, T. Vincent, additional, Silva‐Santos, Bruno, additional, Simon, Anna Katharina, additional, Sitnik, Katarzyna M., additional, Sozzani, Silvano, additional, Speiser, Daniel E., additional, Spidlen, Josef, additional, Stahlberg, Anders, additional, Stall, Alan M., additional, Stanley, Natalie, additional, Stark, Regina, additional, Stehle, Christina, additional, Steinmetz, Tobit, additional, Stockinger, Hannes, additional, Takahama, Yousuke, additional, Takeda, Kiyoshi, additional, Tan, Leonard, additional, Tárnok, Attila, additional, Tiegs, Gisa, additional, Toldi, Gergely, additional, Tornack, Julia, additional, Traggiai, Elisabetta, additional, Trebak, Mohamed, additional, Tree, Timothy I.M., additional, Trotter, Joe, additional, Trowsdale, John, additional, Tsoumakidou, Maria, additional, Ulrich, Henning, additional, Urbanczyk, Sophia, additional, van de Veen, Willem, additional, van den Broek, Maries, additional, van der Pol, Edwin, additional, Van Gassen, Sofie, additional, Van Isterdael, Gert, additional, van Lier, René A.W., additional, Veldhoen, Marc, additional, Vento‐Asturias, Salvador, additional, Vieira, Paulo, additional, Voehringer, David, additional, Volk, Hans‐Dieter, additional, von Borstel, Anouk, additional, von Volkmann, Konrad, additional, Waisman, Ari, additional, Walker, Rachael V., additional, Wallace, Paul K., additional, Wang, Sa A., additional, Wang, Xin M., additional, Ward, Michael D., additional, Ward‐Hartstonge, Kirsten A, additional, Warnatz, Klaus, additional, Warnes, Gary, additional, Warth, Sarah, additional, Waskow, Claudia, additional, Watson, James V., additional, Watzl, Carsten, additional, Wegener, Leonie, additional, Weisenburger, Thomas, additional, Wiedemann, Annika, additional, Wienands, Jürgen, additional, Wilharm, Anneke, additional, Wilkinson, Robert John, additional, Willimsky, Gerald, additional, Wing, James B., additional, Winkelmann, Rieke, additional, Winkler, Thomas H., additional, Wirz, Oliver F., additional, Wong, Alicia, additional, Wurst, Peter, additional, Yang, Jennie H. M., additional, Yang, Juhao, additional, Yazdanbakhsh, Maria, additional, Yu, Liping, additional, Yue, Alice, additional, Zhang, Hanlin, additional, Zhao, Yi, additional, Ziegler, Susanne Maria, additional, Zielinski, Christina, additional, Zimmermann, Jakob, additional, and Zychlinsky, Arturo, additional

Published
2019
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44. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author

Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, Zychlinsky, Arturo, Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. 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W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, and Zychlinsky, Arturo

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Published
2019

45. The potential for bioaugmentation of sand filter materials from waterworks using bacterial cultures degrading 4-chloro-2-methylphenoxyacetic acid

Author

Urse Scheel Krüger, Mette Burmølle, Sebastian R. Sørensen, Anders R. Johnsen, and Jens Aamand

Subjects
Bioaugmentation, Microbiological culture, biology, Environmental remediation, Inoculation, Chemistry, Sand filter, General Medicine, Biodegradation, biology.organism_classification, MCPA, Microbiology, chemistry.chemical_compound, Insect Science, Environmental chemistry, Agronomy and Crop Science, Bacteria
Abstract

BACKGROUND The herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) is found frequently in Danish groundwater in concentrations exceeding the EU threshold limit of 0.1 µg L−1. Groundwater is used for drinking water, and one potential remediation strategy is bioaugmentation using inoculation of sand filters at affected waterworks with degrader bacteria. Numerous bacteria degrading phenoxyacetic acid herbicide have previously been isolated, and they may be candidates for bioaugmentation processes. Designing the optimum inoculum, however, requires knowledge of the capacity for degrading realistically low herbicide concentrations and the robustness of the bacteria when inoculated into sand filter materials. RESULTS Testing a range of different MCPA-mineralising bacterial combinations, using a high-throughput microplate radiorespirometric mineralisation assay, highlighted three efficient cocultures for mineralising low MCPA concentrations. Cocultures demonstrating a shorter time delay before initiation of 14C-ring-labelled MCPA mineralisation to 14CO2, and a more extensive mineralisation of MCPA, compared with those of single strains, were found. When inoculated into different sand filter materials, the coculture effect was diminished, but several single strains enhanced MCPA mineralisation significantly at low MCPA concentrations. CONCLUSION This study shows that an increase in the potential for mineralisation of low herbicide concentrations in sand filter materials can be achieved by inoculating with bacterial degrader cultures. © 2014 Society of Chemical Industry

Published
2014
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48. Degradation of three benzonitrile herbicides by Aminobacter MSH1 versus soil microbial communities: pathways and kinetics

Author

Ulrich Karlson, Nora Badawi, Zuzana Frkova, Nadja Schultz-Jensen, Anders Johansen, Kai Bester, and Sebastian R. Sørensen

Subjects
chemistry.chemical_classification, biology, Bromoxynil, Ecology, Metabolite, General Medicine, Mineralization (soil science), biology.organism_classification, chemistry.chemical_compound, Benzonitrile, Enzyme, chemistry, Aminobacter, Insect Science, Environmental chemistry, Soil water, media_common.cataloged_instance, European union, Agronomy and Crop Science, media_common
Abstract

BACKGROUND: The herbicide dichlobenil was banned in the European Union after its metabolite 2,6-dichlorobenzamide (BAM) was encountered in groundwater. Owing to structural similarities, bromoxynil and ioxynil might be converted to persistent metabolites in a similar manner. To examine this, we used an indigenous soil bacteriumAminobacter sp. MSH1 which is capable of mineralizing dichlobenil via BAM and 2,6-dichlorobenzoic acid (2,6-DCBA). RESULTS: Strain MSH1 converted bromoxynil and ioxynil to the corresponding aromatic metabolites, 3,5-dibromo-4hydroxybenzoic acid (BrAC) and 3,5-diiodo-4-hydroxybenzoic acid (IAC) following Michaelis – Menten kinetics (adjusted R 2 between 0.907 and 0.999). However, in contrast to 2,6-DCBA, degradation of these metabolites was not detected in the pure-culture studies, suggesting that they might pose an environmental risk if similar partial degradation occurred in soil. By contrast, experiments with natural soils indicated 20 – 30% mineralization of ioxynil and bromoxynil within the first week. CONCLUSION: The degradation pathway of the three benzonitriles is initially driven by similar enzymes, after which more specific enzymes are responsible for further degradation. Ioxynil and bromoxynil mineralization in soil is not dependent on previous benzonitrile exposure. The accumulation of dead-end metabolites, as seen for dichlobenil, is not a major problem. c � 2013 Society of Chemical Industry Supportinginformationmaybefoundintheonlineversionofthisarticle.

Published
2014
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49. IL-27 Inhibits Lymphatic Endothelial Cell Proliferation by STAT1-Regulated Gene Expression

Author

Josefine Gibson, Katerina Tritsaris, Michael S. Pepper, Sebastian R. Nielsen, Troels Hammer, Steen Dissing, and Riccardo E. Nisato

Subjects
Physiology, Physiology (medical), Gene expression, Humans, STAT1, Phosphorylation, Molecular Biology, Cells, Cultured, Janus Kinases, biology, Interleukins, Endothelial Cells, Dermis, Chemokine CXCL11, Up-Regulation, Lymphangiogenesis, Chemokine CXCL10, Endothelial stem cell, STAT1 Transcription Factor, Lymphatic system, biology.protein, Cancer research, Fibroblast Growth Factor 2, Endothelium, Lymphatic, Cardiology and Cardiovascular Medicine, Signal Transduction
Abstract

IL-27 belongs to the IL-12 family of cytokines and is recognized for its role in Th cell differentiation and as an inhibitor of tumor angiogenesis. The purpose of this study was to investigate the effect of IL-27 on proliferation of lymphatic endothelial cells to gain insight into the interplay between the immune system and development of the lymphatic system.IL-27-stimulated signal transduction in human dermal lymphatic endothelial cells was measured by western blotting and synthesis of CXCL10 and CXCL11 by use of RT-PCR and ELISA. Proliferation was measured using MTT and BrdU kits and the role of STAT1 and chemokines was determined by use of siRNA and recombinant proteins.Stimulation of lymphatic endothelial cell cultures with IL-27 induced JAK dependent phosphorylation of STAT1 and STAT3 and inhibited lymphatic endothelial cell proliferation and migration. Expression of CXCL10 and CXCL11, both STAT1 target genes, was profoundly up-regulated upon IL-27 stimulation, and recombinant CXCL10 and CXCL11 inhibited FGF-2-induced proliferation in vitro. siRNA targeting of STAT1 almost completely abrogated CXCL10 and CXCL11 expression as well as the proliferative effect of IL-27.IL-27 function as an anti-lymphangiogenic regulator in vitro by up-regulating chemokines and interfering with the mitogenic effect of growth factors through STAT1 activation.

Published
2013
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50. Degradation and mineralisation of diuron by Sphingomonas sp. SRS2 and its potential for remediating at a realistic µg L−1 diuron concentration

Author

René K. Juhler, Jens Aamand, and Sebastian R. Sørensen

Subjects
0303 health sciences, Bioaugmentation, biology, Environmental remediation, General Medicine, 010501 environmental sciences, Dimethylurea, Contamination, Biodegradation, Pesticide, biology.organism_classification, 01 natural sciences, 6. Clean water, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Nutrient, chemistry, Insect Science, Environmental chemistry, Agronomy and Crop Science, Bacteria, 030304 developmental biology, 0105 earth and related environmental sciences
Abstract

Background Low concentrations (10−6–10−9 g L−1) of the herbicide diuron are occasionally detected as water contaminants in areas around the world where the herbicide is used extensively. Remediation of contaminated waters using diuron-mineralising bacteria is a possible approach for cleaning these resources. However, few diuron-mineralising strains have been isolated. Here, the ability of Sphingomonas sp. SRS2, a well-known soil bacterium capable of degrading the structurally related herbicide isoproturon, to mineralise diuron at realistically low concentrations is tested. Results Strain SRS2 readily degraded the dimethylurea side chain, while no or only slow mineralisation of the ring structure was determined. By monitoring metabolites, it was determined that SRS2 initially degraded diuron by two successive N-demethylations followed by cleavage of the urea group to 3,4-dichloroaniline (3,4-DCA). Mineralisation of low diuron concentrations by SRS2 was detected and could be stimulated by the addition of a complex nutrient source. Further enhancement of the mineralisation activity was obtained by combining SRS2 with the 3,4-DCA-mineralising Variovorax sp. SRS16. Conclusion This work demonstrates that Sphingomonas sp. SRS2 is a promising candidate for bioaugmentation, alone or in combination with other strains, and that enhanced diuron mineralisation at realistically low concentrations can be achieved. © 2013 Society of Chemical Industry

Published
2013
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