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14. Determination of daumone in mouse plasma by HPLC/MS-MS.

Author

Noh K, Park JH, Kim M, Jung M, Lee HJ, Kwon KI, Kang W, and Ha H

Subjects
Animals, Drug Stability, Limit of Detection, Linear Models, Male, Mice, Reproducibility of Results, Chromatography, High Pressure Liquid methods, Fatty Acids blood, Pheromones blood, Tandem Mass Spectrometry methods
Abstract

Daumone, a pheromone secreted by Caenorhabditis elegans, is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS-MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5-week repeated oral administration of daumone in mice., (Copyright © 2011 John Wiley & Sons, Ltd.)

Published
2012
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