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Determination of daumone in mouse plasma by HPLC/MS-MS.

Authors :
Noh K
Park JH
Kim M
Jung M
Lee HJ
Kwon KI
Kang W
Ha H
Source :
Biomedical chromatography : BMC [Biomed Chromatogr] 2012 Feb; Vol. 26 (2), pp. 152-5. Date of Electronic Publication: 2011 May 19.
Publication Year :
2012

Abstract

Daumone, a pheromone secreted by Caenorhabditis elegans, is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS-MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5-week repeated oral administration of daumone in mice.<br /> (Copyright © 2011 John Wiley & Sons, Ltd.)

Details

Language :
English
ISSN :
1099-0801
Volume :
26
Issue :
2
Database :
MEDLINE
Journal :
Biomedical chromatography : BMC
Publication Type :
Academic Journal
Accession number :
21594879
Full Text :
https://doi.org/10.1002/bmc.1649