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Understanding persistence of humoral immune responses elicited by vaccination against coronavirus disease 2019 (COVID-19) is critical for informing the duration of protection and appropriate booster timing. We developed a mechanistic model to characterize the time course of humoral immune responses in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-seronegative adults after primary vaccination with the Janssen COVID-19 vaccine, Ad26.COV2.S. The persistence of antibody responses was quantified through mechanistic modeling-based simulations. Two biomarkers of humoral immune responses were examined: SARS-CoV-2 neutralizing antibodies determined by wild-type virus neutralization assay (wtVNA) and spike protein-binding antibodies determined by indirect spike protein enzyme-linked immunosorbent assay (S-ELISA). The persistence of antibody responses was defined as the period of time during which wtVNA and S-ELISA titers remained above the lower limit of quantification. A total of 442 wtVNA and 1,185 S-ELISA titers from 82 and 220 participants, respectively, were analyzed following administration of a single dose of Ad26.COV2.S (5 × 10

The prevalence of diabetes has increased by three folds over the last 20 years, and the global cost of diabetes mellitus surpassed one trillion US Dollars (USD) or 1.8% of the global GDP in 2015. Generally, prescription medication to treat complications of diabetes makes up nearly 30% of diabetes medical expenditures. To facilitate value-based decision-making at national and organizational levels, we analyzed the cost drivers of pharmacy services in a diabetes care institute by developing a flexible costing model that accounts for pharmaceuticals and labour costs of pharmacy processes. We calculated the direct pharmaceutical costs and the indirect labour costs at the activity level from electronic health records and observational data. On average, the cost of pharmacy services over 1 year was equivalent to 1246 USD per diabetes patient. Approximately 98% of the pharmacy costs were pharmaceutical costs, while 2% were attributable to labour. The flexible costing model and cost estimates are essential for value-based comparisons of interventions and care redesign. The outlined costing framework and findings carry implications nationally and organizationally to accelerate the path towards value-based healthcare delivery and provider reimbursement schemes through agile cost estimation, efficiency improvements, and higher value of care.

Evaluation of soil-phosphorus (P) tests is critical to ensure the accuracy of fertilizer recommendations to optimize crop yield while minimizing negative environmental consequences. Olsen-P is the most commonly used soil-P test for alkaline calcareous soils found in Idaho and the Western United States. The Bray-1 test is commonly used in the Pacific Northwest (PNW) on neutral to acidic soils but underestimates P in alkaline calcareous soils. Mehlich-3 has been evaluated throughout various regions in the United States. Little data evaluating the test exists on soils in the Western United States. Additionally, the comparatively newly developed H3A test, a component of the soil health tool, has not been widely evaluated on alkaline calcareous soils. Soil samples from the 0- to 30-cm depth were collected from agricultural fields throughout Idaho and analyzed using Bray-1, H3A, Mehlich-3, and Olsen P extractants. Results suggested that Olsen P was strongly correlated with Mehlich-3, while Bray-1 and H3A were not correlated with Olsen P. Both the Bray-1 and H3A test underestimated extractable P when compared with the Olsen P test, whereas the Mehlich-3 overestimated. A threshold point in calcium carbonate (i.e., inorganic carbon (IC)) of 6.7 and 5.1 mg kg-1 for the Bray-1 and H3A was obtained, respectively, that indicated inorganic carbon concentrations at or above these levels result in underestimation of extractable soil P. Thus, Mehlich-3 was very strongly correlated to Olsen P and could be evaluated for use in alkaline calcareous soils whereas Bray-1 and H3A have notable issues that would limit their applicability.

In this study, an inexpensive Nix Pro (Nix Sensor Ltd.) color sensor was used to develop prediction models for soil iron (Fe) content. Thirty‐eight soil samples were collected from five agricultural fields across the Animas watershed to develop and validate soil Fe prediction models. We used color space models to develop three different parameter sets for Fe prediction with Nix Pro. The different color space sets were used to develop three new predictive models for Nix Pro‐based Fe content against the lab‐based inductively coupled plasma analyzed Fe content. The model performances were assessed using the coefficient of determination, root mean square error, and model p‐value. Three models (International Commission on Illumination's lightness, ±a axis (redness to greenness), and ± b axis (yellowness to blueness) [CIEL*a*b]; red, green, blue [RGB]; and cyan, magenta, yellow, key [black] [CMYK]) were significant in predicting the Fe content using colorimetric variables with R2 ranging from 0.79 to 0.81. The mean square prediction error (MSPE) and Kling–Gupta efficiency (KGE) Index were calculated to validate models and CMYK was predicted to be a better model (MSPE = 0.13; KGE = 0.601) than CIEL*a*b and RGB models. The results suggest Nix Pro is useful in predicting soil Fe content.

Optimizing crop nitrogen (N) uptake while minimizing ammonia (NH3) volatilization from N-fertilizer sources is a critical part of agricultural best management practices. Urea is the most widely used N-fertilizer but also one of the most susceptible to losses as NH3. Fertilizer source and additives can be used to reduce NH3 volatilization. Specifically, urease inhibitors e.g., N-(n-butyl) thiophosphoric triamide (NBPT) can reduce NH3 volatilization from urea. Novel fused ammonium sulfate nitrate (ASN) products have recently been developed as another potential alternative N-fertilizer source but have not been studied widely. A field study was performed to quantify NH3 volatilization from the newly available ASN fertilizer as compared to various N-fertilizers (ammonium sulfate (AS) and urea) including urea treated with the urease inhibitor NBPT in an alkaline calcareous loam soil in Idaho. Further, we assessed NH3 volatilization from surface applied and incorporated N-fertilizers from these various N sources. Untreated surface-applied urea volatilized the highest amount of NH3, whereas incorporation of N-fertilizers was effective in reducing volatilization as compared to untreated surface applied urea. Our study indicated that the N-fertilizer sources (AS, ASN, and Urea-NBPT) were equally effective in reducing NH3 volatilization when compared to surface applied urea. Our study will help refine N-fertilize management under multiple field situations and traditional management practices of crop producers in the western United States. Further, site-years and study locations would be needed to provide evidence of the effectiveness of ASN in reducing NH3 volatilization under a wider range of soil and environmental conditions.

Soil phosphorus testing made great strides with multiple chemical tests proposed and implemented that have been used for fertilizer management programs for crop production during the previous century. In the latter part of the previous century, the environmental impact of excess nonpoint phosphorus loading from the landscape (e.g. agricultural lands) to waterbodies became an issue of increased concern and soil phosphorus testing came to the forefront of management and monitoring. This article will provide a general overview of the usage of phosphorus testing for agronomic purposes in the United States.

The authors surveyed Master Addiction Counselors and members of the International Association of Addictions and Offender Counselors (N = 131) about their use of substance use disorder and process addiction screening and assessment instruments. The results are compared with those of Juhnke, Vacc, Curtis, Coll, and Paredes (2003).

Conventional agricultural practices that use excessive chemical fertilizers and pesticides come at a great price with respect to soil health, a key component to achieve agricultural sustainability. Organic farming could serve as an alternative agricultural system and solve the problems associated with the usage of agro-chemicals by sustainable use of soil resources. A study was carried out to evaluate the impact of organic vs. conventional cultivations of basmati rice on soil health during Kharif (rainy) season of 2011 at Kaithal district of Haryana, India, under farmers' participatory mode. Long-term application of organic residues in certified organic farms was found to improve physical, chemical, and biological indicators of soil health. Greater organic matter buildup as indicated by higher soil organic carbon content in organic fields was critical to increase soil aggregate stability by increasing water holding capacity and reducing bulk density. Proper supplementation of nutrients (both major and micro nutrients) through organic residue addition favored biologically available nutrients in organic systems. Further, the prevalence of organic substrates stimulated soil microorganisms to produce enzymes responsible for the conversion of unavailable nutrients to plant available forms. Most importantly, a closer look at the relationship between physicochemical and biological indicators of soil health evidenced the significance of organic matter to enzyme activities suggesting enhanced nutrient cycling in systems receiving organic amendments. Enzyme activities were very sensitive to short-term (one growing season) effects of organic vs. conventional nutrient management. Soil chemical indicators (organic matter and nutrient contents) were also changed in the short-term, but the response was secondary to the biochemical indicators. Taken together, this study indicates that organic farming practices foster biotic and abiotic interactions in the soil which may facilitate in moving towards a sustainable food future.

Background/Aim Dental avulsion damages periodontal tissues and may induce dentoalveolar ankylosis and root resorption after replantation. The aim of this work was to evaluate the effects of light-emitting diode (LED) therapy after tooth replantation. Material and methods The upper right incisors of 36 male Wistar rats were extracted and replanted after 15 min. The control group (Co, n = 18) was submitted to replantation and the LED group (n = 18) was submitted to replantation and LED therapy (940 nm, 4 J cm−2) for three consecutive days (0, 24, and 48 h). Six animals from each group were euthanized at 7, 14, and 21 days for histological analysis. The upper left incisors were used as a negative control. Data were compared using the Fisher exact test, considering P < 0.05. Results After 7 days, the Co (73.3%) and LED (72.2%) groups presented areas of periodontal ligament necrosis. However, periodontal ligament necrosis was more frequent in the LED group at 14 (70%, P < 0.05) and 21 days (43.2%, P < 0.05) in relation to the Co group (33 and 21.6%). Inflammatory cell infiltration was more frequent in the Co group at 7 days (100%) than the LED group (24.4%, P < 0.05), but increased in the LED group after 21 days (83.3%, P < 0.05). Bone necrosis was more frequent in the LED group after 14 (79%, P < 0.05) and 21 days (60%, P < 0.005) than in the Co group (45% and 20%). Bone repair was evident in the Co group at 14 days. All of the replanted teeth had pulp necrosis. Conclusion The results suggest that the application of LED therapy up to 48 h after tooth replantation may delay periodontal ligament repair.

We distinguish among three types of actions that can be taken to alleviate the consequences of natural disasters: precautionary efforts (made ex ante), relief efforts (made in the immediate aftermath of a disaster), and recovery efforts (made ex post). We argue that recognizing this distinction lessens many of the problems that the literature attributes to government intervention and hence expands the scope of government action following disasters. Relief is less likely than recovery to generate over-supply by the government and over-reliance by victims.