1. Biomass and docosahexaenoic acid formation by Schizochytrium mangrovei Sk-02 at low salt concentrations
- Author
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Saranya Phadungruengluij, Caetharin Assantachai, Panida Unagul, Cornelis Verduyn, Manop Suphantharika, and Thanapol Pongsuteeragul
- Subjects
Magnesium ,Sodium ,chemistry.chemical_element ,Biomass ,Plant Science ,Manganese ,Aquatic Science ,chemistry ,Biochemistry ,Docosahexaenoic acid ,Low salt ,Schizochytrium mangrovei ,Ecology, Evolution, Behavior and Systematics ,Nuclear chemistry - Abstract
Growth and docosahexaenoic acid (DHA) formation in Schizochytrium mangrovei Sk-02 were studied in yeastextract-glucose (YEG) media supplemented with eitherartificial sea salts (ASS) or a simplified salts medium con-sisting of magnesium sulfate and/or sodium chloride.With increasing concentrations of ASS, biomassincreased from 9 (no ASS) to a maximum of 21 g l -1 (at 2.1 M ASS or 68 g l -1 ). Addition of manganeseincreased these values to 17 (no ASS) and a plateau of24gl -1 (at)0.25 M ASS). Replacing ASS withmagnesiumsulfate and/or sodium chloride and manganese as anadditional factor suggested that maximal biomass (24 gl -1 ) could only be achieved when both magnesium sulfateand manganese were added. As significant carry-over ofelements in the inoculum was observed, the latter waswashed in further experiments. Addition of an optimalconcentration of ca. 5 m M magnesium sulfate to YEG-media then resulted in a maximum biomass level of ca.24gl -1 and 5.2 g l -1 DHA in 72 h. Additional supplemen-tation with sodium chloride (8 m
- Published
- 2006
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