1. Acetyl Esterase from Mediterranean Oranges: Partial Purification, Immobilisation and Biotransformations.
- Author
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Pasta, Piero, Verga, Roberto, Zambianchi, Francesca, and Daminati, Moreno
- Subjects
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ESTERASES , *ORANGES , *CITRUS fruits , *CATALYSIS , *HYDROLYSIS , *ENZYMES , *CHEMICAL purification , *CHROMATOGRAPHIC analysis , *POLYACRYLAMIDE gel electrophoresis - Abstract
Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of β-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with ≥98% conversion and 91-93% product yield. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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