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Acetyl Esterase from Mediterranean Oranges: Partial Purification, Immobilisation and Biotransformations.
- Source :
-
Biocatalysis & Biotransformation . May2004, Vol. 22 Issue 3, p221-224. 4p. - Publication Year :
- 2004
-
Abstract
- Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of β-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with ≥98% conversion and 91-93% product yield. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10242422
- Volume :
- 22
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biocatalysis & Biotransformation
- Publication Type :
- Academic Journal
- Accession number :
- 14573287
- Full Text :
- https://doi.org/10.1080/10242420410001697089