1. Dynamics of extended-spectrum cephalosporin resistance genes in Escherichia coli from Europe and North America
- Author
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Zamudio, Roxana, Boerlin, Patrick, Beyrouthy, Racha, Madec, Jean-Yves, Schwarz, Stefan, Mulvey, Michael R, Zhanel, George G, Cormier, Ashley, Chalmers, Gabhan, Bonnet, Richard, Haenni, Marisa, Eichhorn, Inga, Kaspar, Heike, Garcia-Fierro, Raquel, Wood, James LN, Mather, Alison E, Quadram Institute, Biotechnology and Biological Sciences Research Council (BBSRC), University of Guelph, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Centre National de Référence de la Résistance aux Antibiotiques [CHU Clermont-Ferrand] (CNR), CHU Clermont-Ferrand, Unité Antibiorésistance et Virulence Bactériennes (AVB), Laboratoire de Lyon [ANSES], Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Freie Universität Berlin, Veterinary Centre for Resistance Research (TZR), Free University of Berlin (FU), Public Health Agency of Canada, University of Manitoba [Winnipeg], Federal Office of Consumer Protection and Food Safety, University of Cambridge [UK] (CAM), University of East Anglia [Norwich] (UEA), This project was supported by the Joint Programming Initiative on Antimicrobial Resistance (JPIAMR), through the Medical Research Council (MRC, MR/R000948/1) [R.Z., J.L.N.W., A.E.M.], the Canadian Institutes of Health Research (CFC-150770) [A.C., G.C., P.B.], and the Genomics Research and Development Initiative (Government of Canada) [M.R.M.], the German Federal Ministry of Education and Research (BMBF) grant no. 01KI1709 [I.E., H.K., S.S.], the French Agency for food environmental and occupational health & safety (Anses) [R.G.F., M.H., J.Y.M.], and the French National Reference Center (CNR) for antimicrobial resistance [R. Beyrouthy, R. Bonnet]. Support was also provided by the Biotechnology and Biological Sciences Research Council (BBSRC) through the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent project BBS/E/F/000PR10348 (Theme 1, Epidemiology and Evolution of Pathogens in the Food Chain) [R.Z., A.E.M.]., European Project: JPI-EC-AMR, European Project: 323209,EC:FP7:HEALTH,FP7-JPROG-2012-RTD,JPIAMR(2012), Zamudio, Roxana [0000-0003-3634-4828], Schwarz, Stefan [0000-0002-6303-8212], Chalmers, Gabhan [0000-0002-6651-4253], Wood, James LN [0000-0002-0258-3188], Mather, Alison E [0000-0001-6513-3515], and Apollo - University of Cambridge Repository
- Subjects
Multidisciplinary ,Cephalosporin Resistance ,General Physics and Astronomy ,500 Naturwissenschaften und Mathematik::570 Biowissenschaften ,Biologie::570 Biowissenschaften ,Biologie ,General Chemistry ,Antimicrobial resistance ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,beta-Lactamases ,General Biochemistry, Genetics and Molecular Biology ,Anti-Bacterial Agents ,Cephalosporins ,Bacterial genetics ,Escherichia coli ,Animals ,Humans ,Bacterial genomics ,Phylogeny ,Escherichia coli Infections ,Plasmids - Abstract
Funder: Genomics Research and Development Initiative (Government of Canada), the German Federal Ministry of Education and Research (BMBF) grant no. 01KI1709, the French Agency for food environmental and occupational health & safety (Anses), and the French National Reference Center (CNR) for antimicrobial resistance., Extended-spectrum cephalosporins (ESCs) are critically important antimicrobial agents for human and veterinary medicine. ESC resistance (ESC-R) genes have spread worldwide through plasmids and clonal expansion, yet the distribution and dynamics of ESC-R genes in different ecological compartments are poorly understood. Here we use whole genome sequence data of Enterobacterales isolates of human and animal origin from Europe and North America and identify contrasting temporal dynamics. AmpC β-lactamases were initially more dominant in North America in humans and farm animals, only later emerging in Europe. In contrast, specific extended-spectrum β-lactamases (ESBLs) were initially common in animals from Europe and later emerged in North America. This study identifies differences in the relative importance of plasmids and clonal expansion across different compartments for the spread of different ESC-R genes. Understanding the mechanisms of transmission will be critical in the design of interventions to reduce the spread of antimicrobial resistance.
- Published
- 2022