1. Parkia speciosa empty pod extract exerts anti-inflammatory properties by modulating NFκB and MAPK pathways in cardiomyocytes exposed to tumor necrosis factor-α
- Author
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Juriyati Jalil, J. S. Gui, Zakiah Jubri, and Yusof Kamisah
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,medicine.drug_class ,Clinical Biochemistry ,Biomedical Engineering ,Bioengineering ,Inflammation ,Pharmacology ,medicine.disease_cause ,Article ,Anti-inflammatory ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Parkia speciosa ,biology ,Cell Biology ,biology.organism_classification ,Nitric oxide synthase ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Tumor necrosis factor alpha ,medicine.symptom ,Oxidative stress ,Biotechnology - Abstract
Parkia speciosa Hassk is a plant found abundantly in the Southeast Asia region. Its seeds, with or without pods, have been used in traditional medicine locally to treat cardiovascular problems. The pathogenesis of cardiovascular diseases involves inflammation and oxidative stress. Based on this information, we sought to investigate the potential protective effects of Parkia speciosa empty pod extract (PSE) on inflammation in cardiomyocytes exposed to tumor necrosis factor-α (TNF-α). H9c2 cardiomyocytes were divided into four groups; negative control, TNF-α, PSE + TNF-α and quercetin + TNF-α. Groups 3 and 4 were pretreated with PSE ethyl acetate fraction of ethanol extract (500 µg/mL) or quercetin (1000 µM, positive control) for 1 h before inflammatory induction with TNF-α (12 ng/mL) for 24 h. TNF-α increased protein expression of nuclear factor kappa B cell (NFκB) p65, p38 mitogen-activated protein kinase (p38 MAPK), inducible nitric oxide synthase, cyclooxygenase-2 and vascular cell adhesion molecule-1 when compared to the negative control (p
- Published
- 2019