17 results on '"Fazel Shokri"'
Search Results
2. Potential role of viral infection and B cells as a linker between innate and adaptive immune response in systemic lupus erythematosus
- Author
-
Fazel Shokri, Mohammad Farahmand, Sayed Mahdi Marashi, Mohadeseh Zarei Ghobadi, Majid Teymoori-Rad, Shima Izadi, Negar Labbaf, and Sayed-Hamidreza Mozhgani
- Subjects
0301 basic medicine ,Lymphocyte ,Immunology ,Adaptive Immunity ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,immune system diseases ,medicine ,Humans ,Lupus Erythematosus, Systemic ,skin and connective tissue diseases ,B cell ,030203 arthritis & rheumatology ,Autoimmune disease ,B-Lymphocytes ,Innate immune system ,Systemic lupus erythematosus ,Gene Expression Profiling ,medicine.disease ,Acquired immune system ,Immunity, Innate ,030104 developmental biology ,medicine.anatomical_structure ,Virus Diseases ,Primary immunodeficiency - Abstract
Systemic lupus erythematosus (SLE) is an autoimmune disease that involves several organ systems. Although B cells play a key role in SLE pathogenesis, the mechanisms behind B cell dysregulation in SLE development remained controversial. Finding the modules containing highly co-expressed genes in B cells could explain biological pathways involved in the pathogenesis of SLE, which may further support the reasons for the altered function of B cells in SLE disease. A total of three microarray gene expression datasets were downloaded from Gene Expression Omnibus. SLE samples were prepared from the purified B lymphocyte cells of the patients who have not received immunosuppressive drugs as well as high dose immunocytotoxic therapies or steroids. A weighted gene co-expression network was then constructed to find the relevant modules implicated in the SLE progression. Among 17 identified modules, 3 modules were selected through mapping to STRING and finding the ones that had highly connection at the protein level. These modules clearly indicate the involvement of several pathways in the pathogenesis of SLE including viral infection, adaptive immune response, and innate immune response in B lymphocytes. The WGCN analysis further revealed the co-expressed genes involved in both innate and adaptive immune systems. Mix infections and primary immunodeficiency might also dysregulate B lymphocytes, which may facilitate SLE development. As such, identifying novel biomarkers and pathways in lupus would be of importance.
- Published
- 2021
3. Illuminating the in vitro effects of Epstein-Barr virus and vitamin D on immune response in multiple sclerosis patients
- Author
-
Talat Mokhtari-Azad, Majid Teymoori-Rad, Razieh Sadat Kazemi Mozdabadi, Sayed Mahdi Marashi, Mohammad Mehdi Amiri, Mohammad Ali Sahraian, Ahmad Nejati, and Fazel Shokri
- Subjects
Adult ,Male ,0301 basic medicine ,Vitamin ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Multiple Sclerosis ,medicine.disease_cause ,Calcitriol receptor ,Peripheral blood mononuclear cell ,Interferon-gamma ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Immune system ,Virology ,Vitamin D and neurology ,Humans ,Medicine ,Vitamin D ,Cells, Cultured ,business.industry ,Multiple sclerosis ,Immune dysregulation ,medicine.disease ,Interleukin-10 ,030104 developmental biology ,Neurology ,chemistry ,Immunology ,Leukocytes, Mononuclear ,Receptors, Calcitriol ,Female ,Neurology (clinical) ,business ,030217 neurology & neurosurgery ,Immune complex disease - Abstract
Given the complexity of immune complex diseases including multiple sclerosis (MS) and the plausible interactions between different risk factors, delineating the interplay between them would be imperative. The current study aimed to evaluate the in vitro effects of Epstein-Barr virus (EBV) and vitamin D on immune response in MS patients and healthy controls. The status of vitamin D and EBV load was evaluated using multiple techniques. In vitro EBV-infected peripheral blood mononuclear cells (PBMCs), in the presence or absence of vitamin D, were checked for IL-10, IFN-γ, and vitamin D receptor. MS patients showed significantly higher plasma levels of 1,25-(OH)2D but not 25-OHD, increased EBV load, and lower levels of vitamin D receptor (VDR) expression compared with healthy controls. Interestingly, an inverse correlation was observed between VDR expression and EBV load in PBMCs. Indeed, the levels of IFN-γ and IL-10 production were significantly higher in supernatant collected from in vitro EBV-infected PBMCs in MS patients compared with controls. While all vitamin D-treated PBMCs showed reduced levels of IFN-γ production, in vitro treatment of vitamin D showed no influence in IL-10 production. EBV and vitamin D were found to exert opposite in vitro effects on immune dysregulation in these patients. Our results highlight the complex interactions of different risk factors with immune system.
- Published
- 2021
4. Contribution of Fc fragment of monoclonal antibodies to tetanus toxin neutralization
- Author
-
Somayeh Ghotloo, Jalal Khoshnoodi, Mohammad Mehdi Amiri, Fazel Shokri, Ebrahim Abbasi, Mahmood Jeddi-Tehrani, and Forough Golsaz-Shirazi
- Subjects
0301 basic medicine ,Clostridium tetani ,biology ,Toxin ,medicine.drug_class ,Chemistry ,General Neuroscience ,Toxicology ,medicine.disease_cause ,Monoclonal antibody ,Fragment crystallizable region ,Molecular biology ,Neutralization ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Polyclonal antibodies ,medicine ,biology.protein ,Neurotoxin ,Antibody ,030217 neurology & neurosurgery - Abstract
Monoclonal antibodies (MAbs) against neurotoxin of Clostridium tetani are considered as a novel source of immunoglobulins for passive immunotherapy of tetanus. Toxin neutralization is classically attributed to the Fab and F(ab′)2 fragments of antibodies. Herein, we generated Fab and F(ab′)2 fragments of three toxin neutralizing mouse MAbs and compared their neutralizing activities to those of their intact molecules. Fab and F (ab′)2 fragments of the antibodies were generated by papain and pepsin digestions, respectively, and their toxin neutralizing activities were compared with those of the intact antibodies in an in vivo toxin neutralization assay. While low doses of the intact MAbs were able to fully protect the mice against tetanus toxin, none of the mice which received Fab or F(ab′)2 fragments survived until day 14, even at the highest administered dose. All mice receiving human polyclonal anti-tetanus immunoglobulin or their fragments were fully protected. Reduction in toxin neutralization activities of Fab and F(ab′)2 fragments of our MAbs seems to be influenced by their Fc regions. Steric hindrance of the Fc region on the receptor-binding site of the toxin may explain the stronger neutralization of the toxin by the intact MAbs in comparison to their fragments.
- Published
- 2019
5. All-trans retinoic acid in combination with sodium butyrate enhances specific monoclonal antibody productivity in recombinant CHO cell line
- Author
-
Mahmood Rahimi-Zarchi, Mohammad Mehdi Amiri, Fazel Shokri, Seyed Abbas Shojaosadati, and Mahmood Jeddi-Tehrani
- Subjects
0106 biological sciences ,0301 basic medicine ,medicine.drug_class ,Cell Culture Techniques ,Retinoic acid ,Tretinoin ,Bioengineering ,CHO Cells ,Monoclonal antibody ,01 natural sciences ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,Cricetulus ,law ,010608 biotechnology ,medicine ,Animals ,biology ,Chinese hamster ovary cell ,Antibodies, Monoclonal ,Sodium butyrate ,General Medicine ,Transfection ,Molecular biology ,Recombinant Proteins ,030104 developmental biology ,chemistry ,Cell culture ,biology.protein ,Recombinant DNA ,Butyric Acid ,Antibody ,Biotechnology - Abstract
The effects of all-trans retinoic acid (RA) and sodium butyrate (NaBu) on growth, viability and antibody production of two types of transfected Chinese hamster ovary cell lines (CHO-K1 and CHO-S) were investigated using a batch mode cell culture. By adding 0.5 mM NaBu in the CHO-K1 cell culture, the cell specific productivity (Qp) and antibody concentration increased by five- and threefold, respectively. The optimal concentration of RA was 100 nM which resulted in twofold increase in antibody production. In a combination model, RA applied at early growth phase of CHO-K1 cells followed by addition of NaBu with lowering culture temperature at the end of stationary phase resulted in two- and threefold increase in Qp and final antibody concentration, respectively. The latter strategy was also applied on suspended CHO-S cells with enhanced Qp and antibody concentration, but to a lesser extent than the CHO-K1 cells. In conclusion, our results demonstrate that the addition of RA and NaBu along with lowering the culture temperature can increase cell culture period as well as Qp and the final concentration of recombinant monoclonal antibody in both CHO-K1 and CHO-S cells without any significant change in binding affinity of the mAb.
- Published
- 2018
6. Effects of Influenza Derived Peptide on CD8 T Cell Responses to MHC Class I-Restricted Human Telomerase Reverse Transcriptase (hTERT)-Derived Peptide
- Author
-
Seyed Alireza Razavi, Esmat Hashemi, Arezoo Gowhari Shabgah, Gholam Ali Kardar, Fazel Shokri, Jamshid Gholizadeh Navashenaq, and Mir Hadi Seyedzadeh
- Subjects
biology ,010405 organic chemistry ,Chemistry ,Immunogenicity ,Bioengineering ,Major histocompatibility complex ,01 natural sciences ,Biochemistry ,Molecular biology ,Epitope ,0104 chemical sciences ,Analytical Chemistry ,Antigen ,Interferon ,Drug Discovery ,MHC class I ,biology.protein ,medicine ,Molecular Medicine ,Cytotoxic T cell ,Telomerase reverse transcriptase ,medicine.drug - Abstract
Tumor cells in breast cancer are immunogenic and express proteins that can induce immune responses. One important antigen is human telomerase reverse transcriptase (hTERT). The main aim of this study was to use an epitope from hTERT accompanied by an epitope derived from influenza virus restricted to HLA-A2, a common Class I HLA in Iran, to induce T cells against tumoral cells. The epitopes were analyzed in IEDB and EpiMHC databases for the strength of binding to HLA-A2 and immunogenicity, respectively. Peripheral blood mononuclear cells (PBMCs) from 11 HLA-A2-positive breast cancer patients were isolated and then were harvested and co-cultured with MCF-7 cells that were previously trans-loaded with synthesized peptides. PBMC proliferation, interferon-γ (IFN-γ) secretion, and activated T cell cytotoxicity were analyzed by MTT, ELISA, and LDH cytotoxicity assays, respectively. Proliferation of PBMCs incubated with the tumoral peptide was significantly greater than that of controls (P
- Published
- 2018
7. Blocking of opioid receptors in experimental formaline-inactivated respiratory syncytial virus (FI-RSV) immunopathogenesis: from beneficial to harmful impacts
- Author
-
Louis Bont, Mahmood Mahmoodi, Habib Mirzaei, Shahram Shahabi, Fazel Shokri, Alireza Tahamtan, Vahid Salimi, Maryam Golara, Mohammad Gharagozlou, Talat Mokhtari-Azad, Abbas Jamali, Ali Ramezani, and Bagher Minaei
- Subjects
0301 basic medicine ,Microbiology (medical) ,Chemokine ,medicine.drug_class ,Narcotic Antagonists ,viruses ,Immunology ,Respiratory Syncytial Virus Infections ,03 medical and health sciences ,Immune system ,T-Lymphocyte Subsets ,Opioid receptor ,Respiratory Syncytial Virus Vaccines ,Animals ,Immunologic Factors ,Immunology and Allergy ,Medicine ,Receptor ,Lung ,Nalmefene ,Mice, Inbred BALB C ,biology ,medicine.diagnostic_test ,business.industry ,Body Weight ,virus diseases ,General Medicine ,Viral Load ,respiratory system ,Naltrexone ,Disease Models, Animal ,030104 developmental biology ,Bronchoalveolar lavage ,Opioid ,biology.protein ,Cytokines ,business ,Bronchoalveolar Lavage Fluid ,Viral load ,medicine.drug - Abstract
Opioid system plays a significant role in pathophysiological processes, such as immune response and impacts on disease severity. Here, we investigated the effect of opioid system on the immunopathogenesis of respiratory syncytial virus (RSV) vaccine (FI-RSV)-mediated illness in a widely used mouse model. Female Balb/c mice were immunized at days 0 and 21 with FI-RSV (2 × 106 pfu, i.m.) and challenged with RSV-A2 (3 × 106 pfu, i.n.) at day 42. Nalmefene as a universal opioid receptors blocker administered at a dose of 1 mg/kg in combination with FI-RSV (FI-RSV + NL), and daily after live virus challenge (RSV + NL). Mice were sacrificed at day 5 after challenge and bronchoalveolar lavage (BAL) fluid and lungs were harvested to measure airway immune cells influx, T lymphocyte subtypes, cytokines/chemokines secretion, lung histopathology, and viral load. Administration of nalmefene in combination with FI-RSV (FI-RSV + NL-RSV) resulted in the reduction of the immune cells infiltration to the BAL fluid, the ratio of CD4/CD8 T lymphocyte, the level of IL-5, IL-10, MIP-1α, lung pathology, and restored weight loss after RSV infection. Blocking of opioid receptors during RSV infection in vaccinated mice (FI-RSV-RSV + NL) had no significant effects on RSV immunopathogenesis. Moreover, administration of nalmefene in combination with FI-RSV and blocking opioid receptors during RSV infection (FI-RSV + NL-RSV + NL) resulted in an increased influx of the immune cells to the BAL fluid, increases the level of IFN-γ, lung pathology, and weight loss in compared to control condition. Although nalmefene administration within FI-RSV vaccine decreases vaccine-enhanced infection during subsequent exposure to the virus, opioid receptor blocking during RSV infection aggravates the host inflammatory response to RSV infection. Thus, caution is required due to beneficial/harmful functions of opioid systems while targeting as potentially therapies.
- Published
- 2017
8. Hersintuzumab: A novel humanized anti-HER2 monoclonal antibody induces potent tumor growth inhibition
- Author
-
Mohammad Mehdi Amiri, Mohammad Hossein Karimi-Jafari, Fazel Shokri, Reza Hosseini-Ghatar, Tannaz Bahadori, Samira Farid, Mahmood Jeddi-Tehrani, Shadi Sadat Navabi, Tahereh Soltantoyeh, Jalal Khoshnoodi, and Forough Golsaz-Shirazi
- Subjects
Models, Molecular ,0301 basic medicine ,Receptor, ErbB-2 ,medicine.drug_class ,Immunoglobulin Variable Region ,CHO Cells ,Complementarity determining region ,Monoclonal antibody ,Humanized antibody ,Epitope ,03 medical and health sciences ,chemistry.chemical_compound ,Cricetulus ,Trastuzumab ,Cell Line, Tumor ,Cricetinae ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Phosphorylation ,skin and connective tissue diseases ,neoplasms ,Cell Proliferation ,Pharmacology ,Antibody-dependent cell-mediated cytotoxicity ,Antibody-Dependent Cell Cytotoxicity ,Gene Amplification ,Antibodies, Monoclonal ,Cell Cycle Checkpoints ,030104 developmental biology ,Oncology ,chemistry ,Cancer research ,Immunoglobulin Light Chains ,Pertuzumab ,Growth inhibition ,Immunoglobulin Heavy Chains ,Epitope Mapping ,Signal Transduction ,medicine.drug - Abstract
Humanized monoclonal antibodies (mAbs) against HER2 including trastuzumab and pertuzumab are widely used to treat HER2 overexpressing metastatic breast cancers. These two mAbs recognize distinct epitopes on HER2 and their combination induces a more potent blockade of HER2 signaling than trastuzumab alone. Recently, we have reported characterization of a new chimeric mAb (c-1T0) which binds to an epitope different from that recognized by trastuzumab and significantly inhibits proliferation of HER2 overexpressing tumor cells. Here, we describe humanization of this mAb by grafting all six complementarity determining regions (CDRs) onto human variable germline genes. Humanized VH and VL sequences were synthesized and ligated to human γ1 and κ constant region genes using splice overlap extension (SOE) PCR. Subsequently, the humanized antibody designated hersintuzumab was expressed and characterized by ELISA, Western blot and flow cytometry. The purified humanized mAb binds to recombinant HER2 and HER2-overexpressing tumor cells with an affinity comparable with the chimeric and parental mouse mAbs. It recognizes an epitope distinct from those recognized by trastuzumab and pertuzumab. Binding of hersintuzumab to HER2 overexpressing tumor cells induces G1 cell cycle arrest, inhibition of ERK and AKT signaling pathways and growth inhibition. Moreover, hersintuzumab could induce antibody-dependent cell cytotoxicity (ADCC) on BT-474 cells. This new humanized mAb is a potentially valuable tool for single or combination breast cancer therapy.
- Published
- 2017
9. Assessment of the effect of TLR7/8, TLR9 agonists and CD40 ligand on the transformation efficiency of Epstein-Barr virus in human B lymphocytes by limiting dilution assay
- Author
-
Amir Amanzadeh, Mahmood Jeddi-Tehrani, Fazel Shokri, Vahid Younesi, Forough Golsaz Shirazi, and Ali Memarian
- Subjects
Toll-like receptor ,CD40 ,Clinical Biochemistry ,Biomedical Engineering ,TLR9 ,hemic and immune systems ,Bioengineering ,Cell Biology ,Biology ,medicine.disease_cause ,Peripheral blood mononuclear cell ,Epstein–Barr virus ,Molecular biology ,CpG site ,Immunology ,medicine ,biology.protein ,Carcinogenesis ,Receptor ,Original Research ,Biotechnology - Abstract
Infection of human B cells with Epstein-Barr virus (EBV) induces polyclonal activation in almost all infected cells, but a small proportion of infected cells are transformed to immortalized lymphoblastoid cell lines. Since B cells are activated also by CD40 ligand (CD40L) and Toll-like receptor (TLR) agonists via a similar signaling pathway, it is likely that costimulation through these molecules could result in synergistic enhancement of the transformation efficiency of EBV. In this study, the stimulatory effect of TLR7/8 (R848), TLR9 (CpG) agonists and/or CD40L on transformation efficiency of EBV in normal human B cells was assessed using the limiting dilution assay. Costimulation of peripheral blood mononuclear cells (PBMCs) with CpG and R848, but not CD40L, increased significantly the frequency of EBV transformed B cells (p
- Published
- 2013
10. Correction to: Blocking of opioid receptors in experimental formaline-inactivated respiratory syncytial virus (FI-RSV) immunopathogenesis: from beneficial to harmful impacts
- Author
-
Vahid Salimi, Habib Mirzaei, Ali Ramezani, Alireza Tahamtan, Abbas Jamali, Shahram Shahabi, Maryam Golara, Bagher Minaei, Mohammad Javad Gharagozlou, Mahmood Mahmoodi, Louis Bont, Fazel Shokri, and Talat Mokhtari-Azad
- Subjects
Microbiology (medical) ,Immunology ,Immunology and Allergy ,General Medicine - Abstract
In the original publication, seventh author's name was incorrectly published as 'Maryam Golaram'.
- Published
- 2018
11. PCR-based detection and eradication of mycoplasmal infections from various mammalian cell lines: a local experience
- Author
-
Vahid Molla Kazemiha, Shahram Azari, Mohammad Reza Arabestani, Fazel Shokri, Amir Amanzadeh, Morteza Shojaei Moghadam, Mahmood Jeddi Tehrani, Susan Maleki, and Mohammad Ali Shokrgozar
- Subjects
medicine.drug_class ,Clinical Biochemistry ,Antibiotics ,Biomedical Engineering ,Hamster ,Bioengineering ,Cell Biology ,Immunogenetics ,Mycoplasma ,Biology ,medicine.disease_cause ,Virology ,law.invention ,Microbiology ,Ciprofloxacin ,law ,Cell culture ,Mammalian cell ,medicine ,Polymerase chain reaction ,Original Research ,Biotechnology ,medicine.drug - Abstract
A total of 200 cell lines including different human, monkey, mice, hamster and rat cell types were examined for mycoplasma infection status. PCR assay using generic-specific universal primers showed that 40 (20%) of the cell lines are contaminated with mycoplasma. Employment of species-specific primers within these infected cell lines revealed infection with M. hyorhinis (42.5%), M. fermentas (37.5%), M. arginini (37.5%), M. orale (12.5%) and A. laidlawii (7.5%). A number of the cultures were coinfected with 2 or 3 different species. Contaminated samples were treated with BM-Cyclin, Ciprofloxacin and mycoplasma removal agent (MRA). Mycoplasma eradication was subsequently checked by PCR following 2 weeks continuous culture of treated cells in antibiotic free culture medium. Mycoplasmal infections were eradicated in 100, 70 and 42% of infected cell lines when the samples were treated with BM-Cyclin, MRA and Ciprofloxacin, respectively. However, 12% (BM-Cyclin), 62.5% (MRA) and 82.5% (Ciprofloxacin) of mycoplasma regrowth was observed 4 months after the treatment. Notably, the risk of spontaneous culture death was 17.5, 12.5 and 0% for BM-Cyclin, MRA and Ciprofloxacin, respectively.
- Published
- 2009
12. Overexpression of Orphan Receptor Tyrosine Kinase Ror1 as a Putative Tumor-Associated Antigen in Iranian Patients with Acute Lymphoblastic Leukemia
- Author
-
Mahdi, Shabani, Hossein, Asgarian-Omran, Hossein Asgarian, Omran, Mahmood, Jeddi-Tehrani, Parvaneh, Vossough, Mohammad, Faranoush, Ramazan A, Sharifian, Gholam R, Toughe, Mahin, Kordmahin, Jalal, Khoshnoodi, Azam, Roohi, Narjes, Tavoosi, Hakan, Mellstedt, Hodjatallah, Rabbani, and Fazel, Shokri
- Subjects
Adult ,Male ,Neoplasm, Residual ,animal structures ,Iran ,Biology ,Receptor Tyrosine Kinase-like Orphan Receptors ,Tropomyosin receptor kinase C ,Receptor tyrosine kinase ,Immunophenotyping ,Antigens, CD ,Antigens, Neoplasm ,Bone Marrow ,Cell Line, Tumor ,Biomarkers, Tumor ,Humans ,CD135 ,RNA, Messenger ,Child ,Orphan receptor ,Blood Cells ,Reverse Transcriptase Polymerase Chain Reaction ,fungi ,Receptor Protein-Tyrosine Kinases ,General Medicine ,Precursor Cell Lymphoblastic Leukemia-Lymphoma ,Flow Cytometry ,Molecular biology ,Child, Preschool ,embryonic structures ,ROR1 ,biology.protein ,Cancer research ,Female ,Tyrosine kinase ,Platelet-derived growth factor receptor ,Proto-oncogene tyrosine-protein kinase Src - Abstract
Receptor tyrosine kinases (RTKs) are a group of enzymes involved in a variety of physiological and pathological processes. The human Ror1 is a member of the RTK family with unknown ligand and biological function. Overexpression of Ror1 has recently been reported in B-cell chronic lymphocytic leukemia. The aim of this study was to explore the expression profile of Ror1 in acute lymphoblastic leukemia (ALL) cells. Therefore, leukemic cells were isolated from the bone marrow and/or peripheral blood (PB) of 57 ALL patients. Immunophenotyping was performed by flow cytometry and mRNA expression was detected by RT-PCR. Overexpression of Ror1 mRNA was detected in 23 of 57 (40%) ALL patients. A similar expression pattern was observed in ALL cell lines, with 4 of 12 (33%) being positive. Stimulation of normal PB mononuclear cells with pokeweed mitogen and phorbol myristate acetate induced substantially higher Ror1 mRNA expression compared to unstimulated cultured cells. There has been neither a significant association between Ror1 expression and the immunophenotypic profile of the leukemic cells, nor with other clinical or hematological features of the patients. In conclusion, our findings propose Ror1 as a new tumor-associated antigen and a potential tool for targeted immunotherapy and monitoring of minimal residual disease in ALL.
- Published
- 2007
13. The efficient isolation of murine splenic dendritic cells and their cytochemical features
- Author
-
Fazel Shokri, Pouneh Dokouhaki, Jaleh Shojaeian, Mahmood Jeddi-Tehrani, Seyyed-Mohammad Moazzeni, Amir-Hassan Zarnani, and Mojdeh Salehnia
- Subjects
Male ,Histology ,Neutrophils ,medicine.drug_class ,Immunocytochemistry ,Antigen presentation ,Spleen ,Cell Separation ,Lymphocyte proliferation ,In Vitro Techniques ,Biology ,Monoclonal antibody ,Carboxylesterase ,Flow cytometry ,Mice ,In vivo ,medicine ,Animals ,Humans ,Lymphocytes ,Molecular Biology ,Cell Proliferation ,Peroxidase ,Antigen Presentation ,Mice, Inbred BALB C ,medicine.diagnostic_test ,Dendritic Cells ,Cell Biology ,Dendritic cell ,Flow Cytometry ,Immunohistochemistry ,Molecular biology ,CD11c Antigen ,Medical Laboratory Technology ,medicine.anatomical_structure ,Macrophages, Peritoneal - Abstract
Despite their importance in professional antigen presentation and their ubiquitous presence, dendritic cells (DCs) are usually found in such trace amounts in tissues that their isolation with high purity is a difficult task. Because of their scarcity, accurate determination of the purity of isolated dendritic cells is very important. In this study, we purified murine splenic dendritic cells by a three-step enrichment method and evaluated their morphological, cytochemical and functional characteristics. Purity of the isolated cells was determined by established methods such as flow cytometry (FC) and immunocytochemistry (ICC) using anti-CD11c monoclonal antibody. In order to test purified DC functional properties, we used in vivo antigen presentation assay. Our results showed that antigen-pulsed DCs are potent stimulators of antigen-specific lymphocyte proliferation. We studied myeloperoxidase (MPO) and non-specific esterase (NSE) activity in isolated cells to determine the purity of dendritic cells compared to more conventional methods. Our results showed that murine splenic dendritic cells were deficient in both MPO and NSE activity and the percentage of purity obtained by NSE staining on isolated cells was comparable to the results obtained by either FC or ICC. To our knowledge, this is the first report on using NSE activity for determination of the purity of isolated murine splenic dendritic cells. We, therefore, recommend that NSE activity be employed as a simple, inexpensive and yet accurate method for evaluation of the purity of isolated murine splenic dendritic cells.
- Published
- 2006
14. Analysis of the expressed immunoglobulin variable region heavy chain gene products in paraproteins from Iranian patients with multiple myeloma
- Author
-
Rizgar A. Mageed, Ramazan Ali Sharifian, Soheila Gharagozloo, and Fazel Shokri
- Subjects
Male ,Idiotype ,Cancer Research ,medicine.drug_class ,Blotting, Western ,Immunoglobulin Variable Region ,Enzyme-Linked Immunosorbent Assay ,Iran ,Monoclonal antibody ,Pathology and Forensic Medicine ,Gene Frequency ,medicine ,Humans ,Gene family ,Gene ,Multiple myeloma ,Genetics ,biology ,General Medicine ,Middle Aged ,medicine.disease ,Molecular biology ,Myeloma Proteins ,Oncology ,Polyclonal antibodies ,biology.protein ,Female ,Paraproteins ,Antibody ,Immunoglobulin Heavy Chains ,Multiple Myeloma - Abstract
The frequency of expression of immunoglobulin (Ig) variable region heavy (VH ) chain gene products was studied in 43 Iranian patients with mutiple myeloma (MM). The expressed VH gene families and associated cross-reactive idiotypes (CRI) were analysed by immunoblotting and ELISA, using peptide-induced polyclonal antibodies specific for VH 1-VH 6 gene families and monoclonal antibodies (MAb) recognising CRI linked to theVH 1, VH 3, VH 4 and VH 6 gene families. The results revealed that the VH 3 family (60. 5%) was the most predominant gene family. In contrast, no paraproteins were encoded by genes from the VH 2 gene family and only 2.3% were encoded by the VH 5 family. The panel of paraproteins tested rarely expressed the probed VH -associated CRI. Our results suggest that: 1-The Ig VH genes, may not be randomly expressed in the malignant plasma cells from Iranian patients with MM. 2- Some of the genes seem to be negatively selected or highly mutated, as evidenced by the lack of expression of the probed CRI.
- Published
- 2000
15. HLA antigens in iranian patients with B-cell chronic lymphocytic leukemia
- Author
-
Seyed M Moazzeni, Fazel Shokri, and A Amirzargar
- Subjects
Male ,Cancer Research ,business.industry ,Chronic lymphocytic leukemia ,Histocompatibility Antigens Class I ,Histocompatibility Antigens Class II ,Normal population ,General Medicine ,Negative association ,Human leukocyte antigen ,Iran ,Middle Aged ,medicine.disease ,Leukemia, Lymphocytic, Chronic, B-Cell ,Pathology and Forensic Medicine ,Oncology ,Antigen ,Immunology ,Humans ,B-cell chronic lymphocytic leukemia ,Medicine ,Female ,Genetic Predisposition to Disease ,business ,Aged - Abstract
The frequency of HLA class-I and class-II antigens was investigated in 32 Iranian patients with B-cell chronic lymphocytic leukemia (B-CLL), using the microlymphocytotoxicity method. A significant increase in the HLA- B13 (P
- Published
- 1999
16. The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression
- Author
-
Fazel Shokri, Fereydoun Siassi, Seyed Morteza Karimian, Khosro Abdi, Mohammad Hossein Modarressi, Javad Nasrollahzadeh, Javad Mohammadi-Asl, Mahmood Doosti, Arash Nikmanesh, and Mohammad Reza Eshraghian
- Subjects
Chromatography, Gas ,Docosahexaenoic Acids ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Gene Expression ,Nerve Tissue Proteins ,Fatty Acid-Binding Proteins ,Fatty acid-binding protein ,Linoleic Acid ,chemistry.chemical_compound ,Endocrinology ,Cell Line, Tumor ,Glioma ,medicine ,Animals ,Rats, Wistar ,gamma-Linolenic Acid ,adipocyte protein 2 ,gamma-Linolenic acid ,CYP2C8 ,lcsh:RC620-627 ,Biochemistry, medical ,chemistry.chemical_classification ,Retinoid X Receptor alpha ,biology ,Brain Neoplasms ,Reverse Transcriptase Polymerase Chain Reaction ,Research ,Fatty Acids ,Biochemistry (medical) ,Fatty acid ,Genes, erbB-1 ,Lipid Metabolism ,medicine.disease ,Rats ,PPAR gamma ,lcsh:Nutritional diseases. Deficiency diseases ,chemistry ,Biochemistry ,Docosahexaenoic acid ,biology.protein ,Free fatty acid receptor ,Female ,lipids (amino acids, peptides, and proteins) ,Fatty Acid-Binding Protein 7 - Abstract
Background Experimental studies indicate that gamma linolenic acid (GLA) and docosahexaenoic acid (DHA) may inhibit glioma cells growth but effects of oral consumption of these fatty acids on brain tumor fatty acid composition have not been determined in vivo. Methods GLA oil (GLAO; 72% GLA), DHA oil (DHAO; 73% DHA) were fed to adult wistar rats (1 mL/rat/day) starting one week prior to C6 glioma cells implantation and continued for two weeks after implantation. Control group were fed same amount of high linoleic acid safflower oil (74–77% linoleic acid). Fatty acid composition of tumor samples was determined in a set of 8–12 animals in each group and serum fatty acid in 6 animals per each group. Gene expression of tumor fatty acid binding protein 7 (FABP7), epidermal growth factor receptor (EGFR), peroxisome proliferator activated receptor γ (PPAR-γ) and retinoid × receptor-α (RXR-α) were determined in a set of 18 animals per group. Results DHAO feeding increased EPA of brain tumors and decreased ratio of n-6/n-3 fatty acids. Serum levels of EPA were also increased in DHAO group. A similar trend in serum and tumor levels of DHA were observed in DHAO group but it did not achieve statistical significance. GLAO increased serum concentration of GLA but had no significant effect on tumor GLA or dihomo-gamma linolenic acid (DGLA) concentrations. Gene expression of FABP7 was up-regulated in tumors of DHAO group but no other significant effects were observed on EGFR, PPAR-γ or RXR-α expression, and expression of these genes in tumors of GLAO were not different from SFO group. Conclusion Dietary supplementation of DHA containing oil could be an effective way to increase levels of long chain n-3 fatty acids in brain tumors and this increase may be mediated partly by up-regulation of FABP7 expression.
- Published
- 2008
17. Subject Index Vol. 28, 2007
- Author
-
Andrea Faldum, Mohammad Faranoush, Mahdi Shabani, Zhang Cao, Dae Shick Kim, Azam Roohi, Parvaneh Vossough, Michael J. Duffy, Håkan Mellstedt, Martin Sebastian, Shoichi Hazama, Jung Young Lee, Fernando Bittinger, Yutaka Suehiro, Arnold D.K. Hill, Neal D. Hammer, Narjes Tavoosi, G. Kenneth Haines, Mahmood Jeddi-Tehrani, Thomas Karger, Jung-Hyun Yang, Brandon G. Bentz, Lars Henning Schmidt, Fazel Shokri, Jalal Khoshnoodi, David M. Burnett, Roland Buhl, Enda W. McDermott, Slobodanka Klein, Norma O'Donovan, Mikako Hiura, Suk Woo Nam, Eun Yoon Cho, Yuji Hinoda, Su Young Kim, Kohzoh Imai, Jae Hwi Song, Hossein Asgarian Omran, Brett Milash, James A. Radosevich, Masaaki Oka, Seok Jin Nam, Rainer Wiewrodt, Neil A. O'Brien, Gholam R. Toughe, Jung Han Kim, Hodjatallah Rabbani, Deirdre Foley, Kristjan Single, Yoon-La Choi, Christian Taube, Andreas Kuemmel, Koji Ueno, Ramazan Ali Sharifian, Niall O'Higgins, Yong Gu Cho, Won Park, Mahin Kordmahin, Sang Yong Song, Chang Jae Kim, and Young Lyun Oh
- Subjects
Index (economics) ,Statistics ,Subject (documents) ,General Medicine ,Mathematics - Published
- 2007
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.