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All-trans retinoic acid in combination with sodium butyrate enhances specific monoclonal antibody productivity in recombinant CHO cell line
- Source :
- Bioprocess and Biosystems Engineering. 41:961-971
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The effects of all-trans retinoic acid (RA) and sodium butyrate (NaBu) on growth, viability and antibody production of two types of transfected Chinese hamster ovary cell lines (CHO-K1 and CHO-S) were investigated using a batch mode cell culture. By adding 0.5 mM NaBu in the CHO-K1 cell culture, the cell specific productivity (Qp) and antibody concentration increased by five- and threefold, respectively. The optimal concentration of RA was 100 nM which resulted in twofold increase in antibody production. In a combination model, RA applied at early growth phase of CHO-K1 cells followed by addition of NaBu with lowering culture temperature at the end of stationary phase resulted in two- and threefold increase in Qp and final antibody concentration, respectively. The latter strategy was also applied on suspended CHO-S cells with enhanced Qp and antibody concentration, but to a lesser extent than the CHO-K1 cells. In conclusion, our results demonstrate that the addition of RA and NaBu along with lowering the culture temperature can increase cell culture period as well as Qp and the final concentration of recombinant monoclonal antibody in both CHO-K1 and CHO-S cells without any significant change in binding affinity of the mAb.
- Subjects :
- 0106 biological sciences
0301 basic medicine
medicine.drug_class
Cell Culture Techniques
Retinoic acid
Tretinoin
Bioengineering
CHO Cells
Monoclonal antibody
01 natural sciences
law.invention
03 medical and health sciences
chemistry.chemical_compound
Cricetulus
law
010608 biotechnology
medicine
Animals
biology
Chinese hamster ovary cell
Antibodies, Monoclonal
Sodium butyrate
General Medicine
Transfection
Molecular biology
Recombinant Proteins
030104 developmental biology
chemistry
Cell culture
biology.protein
Recombinant DNA
Butyric Acid
Antibody
Biotechnology
Subjects
Details
- ISSN :
- 16157605 and 16157591
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Bioprocess and Biosystems Engineering
- Accession number :
- edsair.doi.dedup.....0f77e2a57038fdf2e904d94f69d63627