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Tidal disruption events (TDEs) occur when stars are ripped apart1,2 by massive black holes and result in highly luminous, multi-wavelength flares3–5. Optical–ultraviolet observations5–7 of TDEs contradict simple models of TDE emission2,8, but the debate between alternative models (for example, shock power9,10 or reprocessed accretion power11–16) remains unsettled, as the dynamic range of the problem has so far prevented ab initio hydrodynamical simulations17. Consequently, past simulations have resorted to unrealistic parameter choices10,12,18–21, artificial mass injection schemes22,23 or very short run-times24. Here we present a three-dimensional radiation-hydrodynamic simulation of a TDE flare from disruption to peak emission, with typical astrophysical parameters. At early times, shocks near pericentre power the light curve and a previously unknown source of X-ray emission, but circularization and outflows are inefficient. Near peak light, stream–disk shocks efficiently circularize returning debris, power stronger outflows and reproduce observed peak optical–ultraviolet luminosities25,26. Peak emission in this simulation is shock-powered, but upper limits on accretion power become competitive near peak light as circularization runs away. This simulation shows how deterministic predictions of TDE light curves and spectra can be calculated using moving-mesh hydrodynamics algorithms.A three-dimensional radiation-hydrodynamic simulation of a tidal disruption event (TDE) flare from disruption to peak emission shows how deterministic predictions of TDE light curves and spectra can be calculated using moving-mesh hydrodynamics algorithms. [ABSTRACT FROM AUTHOR]

The isolation of a patient's metastatic cancer cells is the first, enabling step toward treatment of that patient using modern personalized medicine techniques. Whereas traditional standard-of-care approaches select treatments for cancer patients based on the histological classification of cancerous tissue at the time of diagnosis, personalized medicine techniques leverage molecular and functional analysis of a patient's own cancer cells to select treatments with the highest likelihood of being effective. Unfortunately, the pure populations of cancer cells required for these analyses can be difficult to acquire, given that metastatic cancer cells typically reside in fluid containing many different cell populations. Detection and analyses of cancer cells therefore require separation from these contaminating cells. Conventional cell sorting approaches such as Fluorescence Activated Cell Sorting or Magnetic Activated Cell Sorting rely on the presence of distinct surface markers on cells of interest which may not be known nor exist for cancer applications. In this work, we present a microfluidic platform capable of label-free enrichment of tumor cells from the ascites fluid of ovarian cancer patients. This approach sorts cells based on differences in biomechanical properties, and therefore does not require any labeling or other pre-sort interference with the cells. The method is also useful in the cases when specific surface markers do not exist for cells of interest. In model ovarian cancer cell lines, the method was used to separate invasive subtypes from less invasive subtypes with an enrichment of ~ sixfold. In ascites specimens from ovarian cancer patients, we found the enrichment protocol resulted in an improved purity of P53 mutant cells indicative of the presence of ovarian cancer cells. We believe that this technology could enable the application of personalized medicine based on analysis of liquid biopsy patient specimens, such as ascites from ovarian cancer patients, for quick evaluation of metastatic disease progression and determination of patient-specific treatment. [ABSTRACT FROM AUTHOR]

The three-body problem is arguably the oldest open question in astrophysics and has resisted a general analytic solution for centuries. Various implementations of perturbation theory provide solutions in portions of parameter space, but only where hierarchies of masses or separations exist. Numerical integrations1 show that bound, non-hierarchical triple systems of Newtonian point particles will almost2 always disintegrate into a single escaping star and a stable bound binary3,4, but the chaotic nature of the three-body problem5 prevents the derivation of tractable6 analytic formulae that deterministically map initial conditions to final outcomes. Chaos, however, also motivates the assumption of ergodicity7–9, implying that the distribution of outcomes is uniform across the accessible phase volume. Here we report a statistical solution to the non-hierarchical three-body problem that is derived using the ergodic hypothesis and that provides closed-form distributions of outcomes (for example, binary orbital elements) when given the conserved integrals of motion. We compare our outcome distributions to large ensembles of numerical three-body integrations and find good agreement, so long as we restrict ourselves to 'resonant' encounters10 (the roughly 50 per cent of scatterings that undergo chaotic evolution). In analysing our scattering experiments, we identify 'scrambles' (periods of time in which no pairwise binaries exist) as the key dynamical state that ergodicizes a non-hierarchical triple system. The generally super-thermal distributions of survivor binary eccentricity that we predict have notable applications to many astrophysical scenarios. For example, non-hierarchical triple systems produced dynamically in globular clusters are a primary formation channel for black-hole mergers11–13, but the rates and properties14,15 of the resulting gravitational waves depend on the distribution of post-disintegration eccentricities. The ergodic hypothesis is used to produce a statistical solution to the chaotic non-hierarchical three-body problem. [ABSTRACT FROM AUTHOR]

Microcalcifications are important diagnostic indicators of disease in breast tissue. Tissue microenvironments differ in many aspects between normal and cancerous cells, notably extracellular pH and glycolytic respiration. Hydroxyapatite microcalcification microstructure is also found to differ between tissue pathologies, including differential ion substitutions and the presence of additional crystallographic phases. Distinguishing between tissue pathologies at an early stage is essential to improve patient experience and diagnostic accuracy, leading to better disease outcome. This study explores the hypothesis that microenvironment features may become immortalised within calcification crystallite characteristics thus becoming indicators of tissue pathology. In total, 55 breast calcifications incorporating 3 tissue pathologies (benign – B2, ductal carcinoma in-situ - B5a and invasive malignancy - B5b) from archive formalin-fixed paraffin-embedded core needle breast biopsies were analysed using X-ray diffraction. Crystallite size and strain were determined from 548 diffractograms using Williamson-Hall analysis. There was an increased crystallinity of hydroxyapatite with tissue malignancy compared to benign tissue. Coherence length was significantly correlated with pathology grade in all basis crystallographic directions (P < 0.01), with a greater difference between benign and in situ disease compared to in-situ disease and invasive malignancy. Crystallite size and non-uniform strain contributed to peak broadening in all three pathologies. Furthermore, crystallite size and non-uniform strain normal to the basal planes increased significantly with malignancy (P < 0.05). Our findings support the view that tissue microenvironments can influence differing formation mechanisms of hydroxyapatite through acidic precursors, leading to differential substitution of carbonate into the hydroxide and phosphate sites, causing significant changes in crystallite size and non-uniform strain. [ABSTRACT FROM AUTHOR]

Saxton et al. discuss the multi-wavelength properties and rates of those TDEs and TDE candidates selected via X-ray observations, and van Velzen et al. for those discovered in the optical and ultra-violet part of the spectrum. In addition, because the rate of TDEs can be substantially enhanced in binary black hole systems, TDEs could point us to galaxies that are likely to host so far elusive compact binary SMBHs. TDEs can also occur around active galactic nuclei (AGNs), although uniquely identifying such an event on top of a bright AGN is difficult. [Extracted from the article]

The ability to use Raman spectroscopy to assess tissue in the operative theatre setting could be advantageous for both patients and clinicians. In this study, a method of overcoming the deleterious effects of theatre lighting on the Raman spectra is demonstrated. The effectiveness of this technique is highlighted both within the theatre setting and using human lymph node tissue. The portable MiniRam II Raman spectroscopy device that incorporated a 785-nm laser was used in all experiments. This is an important step towards the use of Raman spectroscopy as a means of analysing sentinel lymph nodes in patients who have been newly diagnosed with breast cancer. [ABSTRACT FROM AUTHOR]

Precession of aligned nuclear spin systems in ions recoiling from the target into vacuum (RIV) with consequent attenuation of angular distributions of emitted radiation is, in principle, a versatile method for measurement of g-factors of nuclear excited states of lifetimes in the pico-second range (Stone et al., Phys. Rev. Lett., 94, 192501, and Stuchbery and Stone, Phys. Rev. C, 76, 034307, ). Calibration of the observed attenuations has been achieved in favourable cases through comparison with measurements on states having previously known g-factors and lifetimes. The general lack of suitable states with known g-factors has limited application of the RIV method. This paper concerns the present status of efforts to describe the states of excited ions recoiling into vacuum in detail so that the average interaction can be estimated with useful precision from a-priori theory. The calculations use the GRASP2K package (Froese-Fischer et al. and Jonsson, Comp. Phys. Comm., 177, 597, & 184, 2197, ) to obtain, for each recoiling ion change state, the individual possible electronic states, their configurations, lifetimes and hyperfine interactions. It is assumed that all possible ionic states are produced, up to a chosen excitation energy. This energy is selected to approximate the energy at which all states have lifetimes far shorter than the nuclear state of interest. It is further assumed that the ionic state total electron angular momenta are randomly oriented in space. The first estimates of the average attenuation of emission distributions, as a function of the product g τ of the nuclear state g-factor and mean lifetime, used an averaged precession frequency obtained neglecting transitions between electronic states. Improved calculations, which include such transitions, are described. [ABSTRACT FROM AUTHOR]

Team sport athletes require a high level of aerobic fitness in order to generate and maintain power output during repeated high-intensity efforts and to recover. Research to date suggests that these components can be increased by regularly performing aerobic conditioning. Traditional aerobic conditioning, with minimal changes of direction and no skill component, has been demonstrated to effectively increase aerobic function within a 4- to 10-week period in team sport players. More importantly, traditional aerobic conditioning methods have been shown to increase team sport performance substantially. Many team sports require the upkeep of both aerobic fitness and sport-specific skills during a lengthy competitive season. Classic team sport trainings have been shown to evoke marginal increases/decreases in aerobic fitness. In recent years, aerobic conditioning methods have been designed to allow adequate intensities to be achieved to induce improvements in aerobic fitness whilst incorporating movement-specific and skill-specific tasks, e.g. small-sided games and dribbling circuits. Such 'sport-specific' conditioning methods have been demonstrated to promote increases in aerobic fitness, though careful consideration of player skill levels, current fitness, player numbers, field dimensions, game rules and availability of player encouragement is required. Whilst different conditioning methods appear equivalent in their ability to improve fitness, whether sport-specific conditioning is superior to other methods at improving actual game performance statistics requires further research. [ABSTRACT FROM AUTHOR]

The native contrast of optical coherence tomography (OCT) data in dense tissues can pose a challenge for clinical decision making. Automated data evaluation is one way of enhancing the clinical utility of measurements. Methods for extracting information from structural OCT data are appraised here. A-scan analysis allows characterization of layer thickness and scattering parameters, whereas image analysis renders itself to segmentation, texture and speckle analysis. All fully automated approaches combine pre-processing, feature registration, data reduction, and classification. Pre-processing requires de-noising, feature recognition, normalization and refining. In the current literature, image exclusion criteria, initial parameters, or manual input are common requirements. The interest of the presented methods lies in the prospect of objective, quick, and/or post-acquisition processing. There is a potential to improve clinical decision making based on automated processing of OCT data. [ABSTRACT FROM AUTHOR]

Near-infrared Raman spectroscopy, an optical technique that is able to interrogate biological tissues, has been used to study bladder and prostate tissues, with the objective being to provide a first approximation of gross biochemical changes associated with the process of carcinogenesis. Prostate samples for this study were obtained by taking a chip at TURP, and bladder samples from a biopsy taken at TURBT and TURP, following ethical approval. Spectra were taken from purchased biochemical constituents and different pathologies within the bladder and the prostate. We were then able to determine the biochemical basis for these pathologies by utilising an ordinary least-squares fit. We have shown for the first time that we are able to utilise Raman spectroscopy in determining the biochemical basis for the different pathologies within the bladder and prostate gland. In this way we can achieve a better understanding of disease processes such as carcinogenesis. This could have major implications in the future of the diagnosis of disease within the bladder and the prostate gland. [ABSTRACT FROM AUTHOR]

Primary hyperparathyroidism (HPT) in 80% of patients is due to a solitary parathyroid adenoma, while in 20% multigland pathology exists, usually hyperplasia [Scott-Coombes, Surgery, 21(12):309-312, 2003]. Despite recent advances in minimally invasive parathyroidectomy, better preoperative localisation techniques and intraoperative parathyroid hormone (PTH) monitoring, a 4% failure rate [Grant CS, Thompson G, Farley D, Arch Surg, 140:47-479, 2005] persists making accurate differentiation between adenomas and hyperplasia of prime importance. We investigated the ability of Raman spectroscopy to accurately differentiate between parathyroid adenomas and hyperplasia. Raman spectra were measured at defined points on the parathyroid tissue sections using a bench-top microscopy system. Multivariate analysis of the spectra was carried out to construct a diagnostic algorithm correlating spectral results with the histopathological diagnosis. A total of 698 spectra were analysed. Principal-component (PCA)-fed linear discriminant analysis (LDA) used to construct a diagnostic algorithm. Detection sensitivity for parathyroid adenomas was 95% and hyperplasia was 93%. These preliminary results indicate that Raman spectroscopy is potentially an excellent tool to differentiate between parathyroid adenomas and hyperplasia. [ABSTRACT FROM AUTHOR]

The Southern Pine Beetle (SPB) is a major pest in southern U.S.A., Mexico, and Central America. Enormous resources in terms of funding, personnel, and effort have been allocated for research and development of pest management tools for this pest over the past century. Access to information from journal articles, reports, fact sheets, etc. does require some effort and is often incomplete and difficult to obtain. The emergence of the Internet now allows rapid access to information from diverse sources. We have created a comprehensive website entitled, 'The Southern Pine Beetle Internet Control Center' (SPBICC) (www.spbicc.vt.edu). The goal of the site is to provide all available resources on SPB to anyone with access to the Internet. It is also designed to increase communication among researchers and professionals to help advance the management of this pest. The site is interactive, which permits constant updating without contacting a webmaster. Attributes of the site include background information in the form of fact sheets, reviews, and government handbooks; an interactive diagnostic identification key for SPB and other bark beetles; a searchable expertise directory; a calendar of SPB-related events; a discussion forum; an online SPB spot growth predictive model; a searchable bibliographic database; a webcrawler designed to search for SPB-related websites, and more. Web-based tools were developed to integrate relational databases as the functional base of this site. The dynamic nature of the SPBICC makes it a powerful tool and a model for development of websites for other major agricultural or forest pests. [ABSTRACT FROM AUTHOR]

The capsids of double-stranded DNA viruses protect the viral genome from the harsh extracellular environment, while maintaining stability against the high internal pressure of packaged DNA. To elucidate how capsids maintain stability in an extreme environment, we use cryoelectron microscopy to determine the capsid structure of thermostable phage P74-26 to 2.8-Å resolution. We find P74-26 capsids exhibit an overall architecture very similar to those of other tailed bacteriophages, allowing us to directly compare structures to derive the structural basis for enhanced stability. Our structure reveals lasso-like interactions that appear to function like catch bonds. This architecture allows the capsid to expand during genome packaging, yet maintain structural stability. The P74-26 capsid has T = 7 geometry despite being twice as large as mesophilic homologs. Capsid capacity is increased with a larger, flatter major capsid protein. Given these results, we predict decreased icosahedral complexity (i.e. T ≤ 7) leads to a more stable capsid assembly. Viral capsids need to protect the genome against harsh environmental conditions and cope with high internal pressure from the packaged genome. Here, the authors determine the structure of the thermostable phage P74-26 capsid at 2.8-Å resolution and identify features underlying enhanced capsid capacity and structural stability. [ABSTRACT FROM AUTHOR]

A tidal disruption event (TDE) ensues when a star passes too close to a supermassive black hole (SMBH) in a galactic center, and is ripped apart by its tidal field. The gaseous debris produced in a TDE can power a bright electromagnetic flare as it is accreted by the SMBH; so far, several dozen TDE candidates have been observed. For SMBHs with masses above ∼107M⊙, the tidal disruption of solar-type stars occurs within ten gravitational radii of the SMBH, implying that general relativity (GR) is needed to describe gravity. Three promising signatures of GR in TDEs are: (1) a super-exponential cutoff in the volumetric TDE rate for SMBH masses above ∼108M⊙ due to direct capture of tidal debris by the event horizon, (2) delays in accretion disk formation (and a consequent alteration of the early-time light curve) caused by the effects of relativistic nodal precession on stream circularization, and (3) quasi-periodic modulation of X-ray emission due to global precession of misaligned accretion disks and the jets they launch. We review theoretical models and simulations of TDEs in Newtonian gravity, then describe how relativistic modifications give rise to these proposed observational signatures, as well as more speculative effects of GR. We conclude with a brief summary of TDE observations and the extent to which they show indications of these predicted relativistic signatures. [ABSTRACT FROM AUTHOR]