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Alt?s three-dimensional cell balance equation characterizing the chemotactic bacteria was analyzed under the presence of one-dimensional spatial chemoattractant gradients. Our work differs from that of others who have developed rather general models for chemotaxis in the use of a non-smooth anisotropic tumbling frequency function that responds biphasically to the combined temporal and spatial chemoattractant gradients. General three-dimensional expressions for the bacterial transport parameters were derived for chemotactic bacteria, followed by a perturbation analysis under the planar geometry. The bacterial random motility and chemotaxis were summarized by a motility tensor and a chemotactic velocity vector, respectively. The consequence of invoking the diffusion-approximation assumption and using intrinsic one-dimensional models with modified cellular swimming speeds was investigated by numerical simulations. Characterizing the bacterial random orientation after tumbles by a turn angle probability distribution function, we found that only the first-order angular moment of this turn angle probability distribution is important in influencing the bacterial long-term transport. [ABSTRACT FROM AUTHOR]

Abstract  Vicinity analysis (VA) is a new methodology developed to identify similarities between protein binding sites based on their three-dimensional structure and the chemical similarity of matching residues. The major objective is to enable searching of the Protein Data Bank (PDB) for similar sub-pockets, especially in proteins from different structural and biochemical series. Inspection of the ligands bound in these pockets should allow ligand functionality to be identified, thus suggesting novel monomers for use in library synthesis. VA has been developed initially using the ATP binding site in kinases, an important class of protein targets involved in cell signalling and growth regulation. This paper defines the VA procedure and describes matches to the phosphate binding sub-pocket of cyclin-dependent protein kinase 2 that were found by searching a small test database that has also been used to parameterise the methodology. [ABSTRACT FROM AUTHOR]

Purpose: To determine the relative contributions of patient, disease and therapy specific factors on neurocognitive outcome of brain tumor patients.Patients and methods: Seventy-nine patients (mean age = 41.1 years; range: 17–75 years; 54% male, 46% female) with glioblastoma multiforme (37%), anaplastic astrocytoma (17%), low grade astrocytoma (13%), and oligodendroglioma (10%) predominantly in the frontal regions (45%) were evaluated in an outpatient neuro-oncology clinic. A neuropsychological test battery emphasized elements of attention/concentration. Multiple regression analyses determined relationships between functional outcomes and demographic and clinical predictors.Results: Key predictors of neurocognitive functioning included age of the patient (36–59 years, p < 0.01; ≥60 years, p < 0.05) and frontal region tumor location (p < 0.01). As expected, older patients did not perform as well as younger patients in absolute terms on neuropsychological tests; decrements persisted when comparisons were based on age-standardized versions of neurocognitive outcomes. Major depressive disorder was marginally associated with outcomes, while surgical interventions and radiotherapy did not show strong associations with test performances.Conclusions: Primary malignant brain disease was found to be less negative on neurocognitive outcomes for younger than for either middle-aged or older patients. Treatments were not as predictive of neurocognitive outcomes as age. No single test outcome measure was as sensitive to neurocognitive status as the empirically derived index of attention and concentration. [ABSTRACT FROM AUTHOR]

Endemic to southern Australia, the highly venomous octopus Hapalochlaena fasciata has extended its distribution range to temperate areas including Korea, raising concern for potential poisoning risks associated with consuming or encountering these tetrodotoxin (TTX)-bearing octopuses. We assessed TTX and its analogues in blue-lined octopuses occurring on Jeju Island, Korea using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The mitochondrial 16S ribosomal RNA (16S rRNA) and cytochrome oxidase subunit I (COI) sequences analysis revealed that the octopus specimens collected from Jeju Island were H. fasciata. The immunohistochemistry revealed that TTX is mostly distributed in the posterior salivary gland (PSG). In the LC-MS/MS, the PSG showed the highest content of TTX, ranging from 259.4 to 883.5 µg/g, which was several hundred times higher than the levels observed from the skin (2.5–32.0 µg/g) and arms and mantle (2.9–19.0 µg/g). Using LC-Orbitrap MS, we also identified and quantified the TTX analogues. High concentrations of TTX analogues were confirmed in PSG, showing a parallel to the presence of TTX. In PSG, deoxyTTXs (343 ± 269 µg/g) were found to be highest, followed by dideoxyTTX (144 ± 96 µg/g), 4-epiTTX (21.1 ± 12.3 µg/g), trideoxyTTX (14.4 ± 28.8 µg/g), and anhydroTTX (6.82 ± 3.86 µg/g). The levels of TTX and its analogues found in H. fasciata specimens collected from Jeju Island were comparable to those reported in venomous octopuses from tropical and subtropical areas. As sea surface temperatures (SST) rise in temperate regions, there is a potential for an increase in the prevalence of venomous octopuses. Therefore, conducting regular surveillance of such toxic organisms is imperative to ensure public health safety and effective management strategies. [ABSTRACT FROM AUTHOR]

The editorial discusses the challenges of reimbursement for 3D printing in United States Health Care Facilities (HCFs) due to operating expenses exceeding collections. It proposes new technology Ambulatory Payment Classification (APC) codes to fairly reimburse 3D printing services based on technical complexity levels. A survey conducted by the American College of Radiology revealed widespread use of 3D printing in US healthcare, with various specialties utilizing patient-specific 3D printed parts. The editorial recommends categorizing 3D printing complexity into Basic, Intermediate, and Complex levels to guide reimbursement and ensure appropriate use of resources. [Extracted from the article]

The early microbiota of neonates is crucial for developing the postnatal immune system and establishing normal physiological, metabolic, and neurological functions. This study aimed to investigate the factors influencing the diversity of the neonatal oral microbiome, including mother-to-newborn microbial transmission. The study includes a prospective cohort comprising 73 mothers and 87 neonates and a retrospective cohort comprising 991 mothers and 1,121 neonates. Samples from the maternal cervix and neonatal gastric, bronchial, and oral cavities were analyzed using culture-based methods. Neonatal oral swab samples were also analyzed using 16S rRNA gene sequencing to characterize microbial diversity and composition. Similar genera were detected in the neonatal gastric, bronchial, and oral samples, and the neonatal gastric culture was the most similar to the maternal cervical culture. In addition to mother-to-newborn microbial transmission, various natal factors including birth type, birth weight, delivery mode, maternal chorioamnionitis, maternal diabetes and the presence of microbes in other sites influenced neonatal oral microbiome diversity. Among these factors, the birth type was the most significant, and preterm neonates exhibited decreased oral microbiome diversity, with fewer beneficial bacteria and more pathogens. These findings could serve as a baseline for research on the establishment of the oral microbiota in preterm neonates and its health implications. [ABSTRACT FROM AUTHOR]

Purpose of Review: The purpose of the current review is to analyze the current literature regarding the tools available to evaluate patient expectations and satisfaction. There have been an increasing number of tools that have been developed and validated for various orthopedic procedures. Despite the growing number of tools, there are a limited number of tools available for pediatric patients. Recent Findings: Several tools have been developed in orthopedics to evaluate patient expectations. However, there are no tools that have been validated in the pediatric population. In addition, pediatric patient expectations should be collected in conjunction with parent/caregiver expectations. Although not specifically validated for pediatric patients, there are several tools available that may pertain to pediatric patients including the HSS ACL Expectations Survey, HSS Shoulder Expectations Survey, HSS Knee Surgery Expectations Survey, HSS Foot and Ankle Surgery Expectation Survey, Sunnybrook Surgery Expectations Survey, Musculoskeletal Outcomes Data Evaluation and Management System (MODEMS) Instruments, Quick DASH, and DASH. In terms of patient satisfaction, there are even fewer tools available. Several tools have been developed to evaluate patient satisfaction and five additional tools within orthopedics. Of these tools, there are two that have been validated for pediatric patients: The Swedish Parents Satisfaction Questionnaire and the Scoliosis Research Society-22. Summary: There are a growing number of tools to evaluate patient's expectations and satisfaction in the orthopedic literature. Given most of these tools pertain to adult patients, there is a need for further development of tools specifically validated for pediatric patients and their parents/caregivers. Through the measurement of expectations and satisfaction, medical professionals can hope to improve satisfaction and outcomes. [ABSTRACT FROM AUTHOR]

In this paper, we present a groundbreaking approach to enhance the illumination of traffic boards along superhighways, addressing significant challenges associated with conventional lighting systems. Our innovative method revolves around the strategic placement of remote spotlights at the side of the roadway to illuminate traffic signs equipped with retroreflector film (RTRF). The essence of our approach lies in remote illumination, which requires meticulous adjustment of the divergence angle of the spotlights to match the size of the signs and their distance from the projection source. To achieve the desired spotlight configuration, we have developed a hybrid optical system that incorporates a paraboloid reflector and a lens mounted on a bridge holder situated on top of the mirror. Through spot light illumination, we discovered that the initial divergence angle of the RTRF was too narrow. To improve projection angle tolerance, we recommend attaching a light diffuser film onto the surface of the RTRF. The coverage area ratio of the diffuser film can be adjusted to select the desired divergence angle for the reflected light. Our experimental measurements have yielded significant results, showcasing the half-divergent angle of the RTRF ranging from 3° to 7° for different coverage area ratios of the diffuser. In practical terms, with a target luminance of 300 nits at the white word on the traffic board, the power consumption of the spotlight fixture of the roadway was only 40 W, representing over 75% power savings when compared to traditional lighting methods. Consequently, our approach opts to utilize spotlights for illuminating specific traffic boards on superhighways, offering a more efficient and manageable lighting solution that greatly benefits both motorists and road maintenance personnel. [ABSTRACT FROM AUTHOR]

Rapid restoration of access to essential goods and services has long been regarded as paramount for community recovery. Yet, there remains ambiguity in how access should be defined, measured, or operationalized. Defining accessibility as the ability to use available goods and services with a reasonable level of effort and cost requires evaluating it across six dimensions (proximity, availability, adequacy, acceptability, affordability, and awareness) while considering the perspective of both users and providers in the evaluation. But common distance-based metrics that focus solely on physical access and travel time often fall short of fully capturing these requirements, overlooking the user's perception. This paper introduces a new spatio-temporal accessibility metric that combines four out of these six dimensions, including proximity, acceptability, adequacy, and availability. The metric considers uncertainty in measuring each dimension and addresses both user and provider perspectives in measuring the acceptability and adequacy dimensions. The variation in the metric across the disaster timeline serves as a proxy for community recovery. The metric aligns with common engineering-oriented functionality-based resilience frameworks as the functionality level of the providers has been incorporated in its development. Operating at the household level, the metric determines the ratio of post-disruption access time to the intended good or service against its pre-disruption access time and yields a unitless ratio between zero and one, with zero expressing a total loss in accessibility and one signifying the same level of accessibility as pre-disruption. The proposed metric, while being scientifically principled, is a practical tool whose output is easily understood even by non-expert individuals. The metric is illustrated for schools and pharmacies using the Lumberton Testbed and data collected following the 2016 flood in Lumberton, North Carolina after Hurricane Matthew. Findings provide new insight into recovery plan prioritization and can be used to trigger protective actions. The paper concludes by discussing issues and barriers related to developing and validating accessibility metrics while highlighting areas for future research. [ABSTRACT FROM AUTHOR]

Maternal nutrition significantly influences fetal development and postnatal outcomes. This study investigates the impact of maternal overfeeding during mid to late pregnancy on gene expression in the round and sirloin muscles of Hanwoo neonatal calves. Eight cows were assigned to either a control group receiving standard nutrition (100%) or a treated group receiving overnutrition (150%). After birth, tissue samples from the round and sirloin muscles of neonatal calves were collected and subjected to RNA sequencing to assess differentially expressed genes (DEGs). RNA sequencing identified 43 DEGs in round muscle and 15 in sirloin muscle, involving genes related to myogenesis, adipogenesis, and energy regulation. Key genes, including PPARGC1A, THBS1, CD44, JUND, CNN1, ENAH, and RUNX1, were predominantly downregulated. Gene ontology (GO) enrichment analyses revealed terms associated with muscle development, such as "biological regulation," "cellular process," and "response to stimulus." Protein-protein interaction networks highlighted complex interactions among DEGs. Random Forest analysis identified ARC, SLC1A5, and GNPTAB as influential genes for distinguishing between control and treated groups. Overall, maternal overnutrition during mid-to-late pregnancy results in the downregulation of genes involved in muscle development and energy metabolism in neonatal Hanwoo calves. These findings provide insights into the molecular effects of maternal nutrition on muscle development. [ABSTRACT FROM AUTHOR]

Background: Myeloid sarcoma (MS) is an extramedullary tumor constituted by myeloid blasts or immature myeloid cells. It frequently occurs in conjunction with acute myeloid leukemia (AML); however, it can exceptionally manifest in patients without leukemia. Here, we present a rare case of primary MS originating in the small bowel without evidence of bone marrow involvement. Case representation: A 33 year-old female with no relevant medical history was admitted to our hospital with recurrent abdominal pain. Computed tomography (CT) revealed bowel obstruction due to thickening of the ileum wall, which was suspected to be an ileal tumor. Initially, ectopic endometriosis was suspected because of abdominal pain associated with the menstrual cycle and changes observed on a follow-up CT scan. The lesion could not be detected by double-balloon endoscopy. Despite conservative treatment, the obstruction persisted, and laparoscopic partial ileal resection was performed, which revealed extensive involvement of the ileum and mesentery. Additionally, the mesentery of the resected ileum was extremely thickened. Histopathological and immunohistochemical examinations of the surgical specimen indicated ileal MS. Bone marrow aspiration after discharge was negative for cytological findings of leukemia, leading to a final diagnosis of primary ileal MS. Her postoperative course was uneventful, and she is currently undergoing systemic chemotherapy tailored to AML at another hospital. Conclusions: Even though MS of the small bowel is rare and may not be considered preoperatively, similar surgical treatment to that of other small bowel malignancies can ensure proper postoperative diagnosis and appropriate chemotherapy. Given the potential need for chemotherapy, ensuring surgical safety that allows for its rapid initiation is critical. [ABSTRACT FROM AUTHOR]

Agricultural systems strongly impact ecosystems by driving terrestrial degradation, water depletion, and climate change. The Life Cycle Assessment allows for comprehensive analyses of the environmental impacts of food production. Nonetheless, its application still faces challenges due to cropping systems' increased complexity and multifunctionality. Past research has emphasized the need for more holistic approaches to consider dynamic crop interactions and diverse functions of cropping systems, beyond just meeting the demand for foods and feeds. In this context, this study applied an alternative combined and multifunctional modelling approach to compare the environmental performances of two durum wheat cropping systems. The latter differed in crop rotation schedules, farming methods, tillage techniques, and genotypes grown (including both modern and old ones). Novel methodological choices were adopted in this study, aiming at best representing the complexity and peculiarities of these systems, by considering crop rotation effects and reflecting the main durum wheat stakeholders' perspectives. The results showed that the organic low-input landrace-growing system (Case 1) had considerably lower environmental impacts than the conventional high-input one (Case 2), regardless of the functional unit. The environmental hotspots were the increased land occupation and the bare fallow for Case 1 and Case 2, respectively. At the endpoint level, the most affected impact categories for both the systems of analysis were land use, fine particulate matter formation, global warming (human health), and human non-carcinogenic toxicity. Also, the midpoint analysis pointed out important differences in terms of other assessed impact categories, with Case 1 better performing for the majority of them. The identified improvement solutions include the following: the enhancement of the yield performances and the optimization of nitrogen provision from the leguminous crop for Case1, the shift toward a more efficient rotational scheme, the reduction of the use of external inputs, and the avoidance of unnecessary soil tillage operations for Case 2. [ABSTRACT FROM AUTHOR]

Background: Previous observational studies have indicated a complex association between gut microbiota (GM) and neuropathic pain (NP). Nonetheless, the precise biological mechanisms underlying this association remain unclear. Therefore, we adopted a Mendelian randomization (MR) approach to investigate the causal relationship between GM and neuropathic pain including post-herpetic neuralgia (PHN), painful diabetic peripheral neuropathy (PDPN), and trigeminal neuralgia (TN), as well as to explore the potential mediation effects of immune cells. Methods: We performed a two-step, two-sample Mendelian randomization study with an inverse variance-weighted (IVW) approach to investigate the causal role of GM on three major kinds of NP and the mediation effect of immune cells between the association of GM and NP. In addition, we determine the strongest causal associations using Bayesian weighted Mendelian randomization (BWMR) analysis. Furthermore, we will investigate the mediating role of immune cells through a two-step Mendelian randomization design. Results: We identified 53 taxonomies and pathways of gut microbiota that had significant causal associations with NP. In addition, we also discovered 120 immune cells that exhibited significant causal associations with NP. According to the BWMR and two-step Mendelian randomization analysis, we identified the following results CD4 on CM CD4 + (maturation stages of T cell) mediated 6.7% of the risk reduction for PHN through the pathway of fucose degradation (FUCCAT.PWY). CD28 + DN (CD4-CD8-) AC (Treg) mediated 12.5% of the risk reduction for PHN through the influence on Roseburia inulinivorans. CD45 on lymphocyte (Myeloid cell) mediated 11.9% of the risk increase for TN through the superpathway of acetyl-CoA biosynthesis (PWY.5173). HLA DR + CD8br %T cell (TBNK) mediated 3.2% of the risk reduction for TN through the superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (PWY.7323). IgD-CD38-AC (B cell) mediated 7.5% of the risk reduction for DPN through the pathway of thiazole biosynthesis I in E. coli (PWY.6892). Discussion: These findings provided evidence supporting the causal effect of GM with NP, with immune cells playing a mediating role. These findings may inform prevention strategies and interventions directed toward NP. Future studies should explore other plausible biological mechanisms. [ABSTRACT FROM AUTHOR]

Anomalous diffusion in the presence or absence of an external force field is often modelled in terms of the fractional evolution equations, which can involve the hyper-singular source term. For this case, conventional time stepping methods may exhibit a severe order reduction. Although a second-order numerical algorithm is provided for the subdiffusion model with a simple hyper-singular source term t μ , - 2 < μ < - 1 in [arXiv:2207.08447], the convergence analysis remain to be proved. To fill in these gaps, we present a simple and robust smoothing method for the hyper-singular source term, where the Hadamard finite-part integral is introduced. This method is based on the smoothing/IDm-BDFk method proposed by Shi and Chen (SIAM J Numer Anal 61:2559–2579, 2023) for the subdiffusion equation with a weakly singular source term. We prove that the kth-order convergence rate can be restored for the diffusion-wave case γ ∈ (1 , 2) and sketch the proof for the subdiffusion case γ ∈ (0 , 1) , even if the source term is hyper-singular and the initial data is not compatible. Numerical experiments are provided to confirm the theoretical results. [ABSTRACT FROM AUTHOR]

Climate change is a constant global challenge. An approach to help mitigate climate change is carbon capture and utilization (CCU), in which captured CO2 is reused as raw material for consumer products. Because innovations like CCU are unfamiliar to the general public, their communication is critical for a successful rollout. To date, sustainability innovation research has largely neglected the empirical study of communication. The present study contributes to studying the information and communication needs of laypeople based on perceptions and acceptance patterns for CCU by focusing on acceptance profiles for CCU-based insulation boards. In an empirical two-step approach, a qualitative interview prestudy was followed by a quantitative questionnaire measurement (N = 643). Using k-means clustering, the respondents were divided into three acceptance groups: rejecters (15%), tentative accepters (51%), and strong accepters (34%). Analysis showed that regarding their demographics and personality traits, tentative accepters and rejecters were similar. All segments trusted science and health experts best, and only the rejecters distrusted some specific actors. Information on the product's risks and functional properties was most important for all acceptance groups. Based on the study's insights, both general and targeted managerial communication and policy guidelines were formulated. [ABSTRACT FROM AUTHOR]

In this manuscript, a nonlinear time-fractional diffusion equation with a generalized memory kernel is studied. Initially, the original model problem is linearized by implementing the Newton's quasilinearization technique. In the time-fractional term, a generalized Caputo derivative is considered and approximated using the non-uniform L1-scheme as the solution has a singularity at t = 0 . The main contribution of this work is to develop a generalized discrete fractional Grönwall inequality. Thereafter, permitting its use to establish the stability and analyze the error estimate, under a proper regularity condition in the L 2 -norm, and an optimal convergence order O N - (2 - ζ) is obtained for the L1-scheme with respect to the graded mesh. Numerical results are inserted to corroborate the effectiveness of the theoretical analysis. [ABSTRACT FROM AUTHOR]

As the adoption of explainable AI (XAI) continues to expand, the urgency to address its privacy implications intensifies. Despite a growing corpus of research in AI privacy and explainability, there is little attention on privacy-preserving model explanations. This article presents the first thorough survey about privacy attacks on model explanations and their countermeasures. Our contribution to this field comprises a thorough analysis of research papers with a connected taxonomy that facilitates the categorization of privacy attacks and countermeasures based on the targeted explanations. This work also includes an initial investigation into the causes of privacy leaks. Finally, we discuss unresolved issues and prospective research directions uncovered in our analysis. This survey aims to be a valuable resource for the research community and offers clear insights for those new to this domain. To support ongoing research, we have established an online resource repository, which will be continuously updated with new and relevant findings. [ABSTRACT FROM AUTHOR]

This paper proposes the design of a metasurface for polarization and incident angle-insensitive RCS reduction applications. An ellipse-shaped unit cell is utilized as a polarization converter, which is then arranged to form a checkerboard surface. While a single layer checkerboard structure gives a wideband RCS reduction, a double layer structure yields polarization and incident angle independent operation. The two layers have unit cells rotated 450 to each other. Experimental results demonstrate an RCS reduction bandwidth of around 90%. Further the RCS reduction remains stable with polarization and incident angle variation. [ABSTRACT FROM AUTHOR]

Serverless computing is one of the recent compelling paradigms in cloud computing. Serverless computing can quickly run user applications and services regardless of the underlying server architecture. Despite the availability of several commercial and open-source serverless platforms, there are still some open issues and challenges to address. One of the key concerns in serverless computing platforms is security. Therefore, in this paper, we present a multi-layer abstract model of serverless computing for an security investigation. We conduct a quantitative analysis of security risks for each layer. We observe that the Attack Tree and Attack-Defense Tree methodologies are viable approaches in this regard. Consequently, we make use of the Attack Tree and the Attack-Defense Tree to quantify the security risks and countermeasures of serverless computing. We also propose a novel measure called the Relative Risk Matrix (RRM) to quantify the probability of attack success. Stakeholders including application developers, researchers, and cloud providers can potentially apply these findings and implications to better understand and further enhance the security of serverless computing. [ABSTRACT FROM AUTHOR]

Background: Lung adenocarcinoma (LUAD) is still one of the most prevalent malignancies. Interleukin factors are closely associated with the initiation and progression of cancer. However, the relationship between interleukin factors and LUAD has not been fully elucidated. This study aimed to use Mendelian randomization (MR) and RNA sequencing (RNA-seq) analyses to identify the interleukin factors associated with the onset and progression of LUAD. Methods: Exposure-related instrumental variables were selected from interleukin factor summary datasets. The LUAD summary dataset from FINGENE served as the outcome. MR and sensitivity analyses were conducted to screen for interleukin factors associated with LUAD occurrence. Transcriptome analyses revealed the role of interleukin factors in lung tissues. The results were validated through Western blotting and further confirmed with driver gene-negative patients from multiple centers. Potential mechanisms influencing LUAD occurrence and development were explored using bulk RNA-seq and single-cell RNA-seq data. Results: MR analysis indicated that elevated plasma levels of IL6RB, IL27RA, IL22RA1, and IL16 are causally associated with increased LUAD risk, while IL18R1 and IL11RA exhibit the opposite effect. Transcriptome analyses revealed that IL11RA, IL18R1, and IL16 were downregulated in tumor tissues compared with normal lung tissue, but only higher expression of IL11RA correlated with improved prognosis in patients with LUAD from different centers and persisted even in driver-gene negative patients. The IL11RA protein level was lower in various LUAD cell lines than in human bronchial epithelial cells. The genes co-expressed with IL11RA were enriched in the Ras signaling pathway and glycosylation processes. Fibroblasts were the primary IL11RA-expressing cell population, with IL11RA+fibroblasts exhibiting a more immature state. The genes differentially expressed between IL11RA+and IL11RA- fibroblasts were involved in the PI3K-Akt/TNF signaling pathway. Conclusion: According to the MR and transcriptome analyses, the downregulation of IL11RA was closely related to the occurrence and development of LUAD. [ABSTRACT FROM AUTHOR]

Imaging the internal architecture of fast-vibrating structures at micrometer scale and kilohertz frequencies poses great challenges for numerous applications, including the study of biological oscillators, mechanical testing of materials, and process engineering. Over the past decade, X-ray microtomography with retrospective gating has shown very promising advances in meeting these challenges. However, breakthroughs are still expected in acquisition and reconstruction procedures to keep improving the spatiotemporal resolution, and study the mechanics of fast-vibrating multiscale structures. Thereby, this works aims to improve this imaging technique by minimising streaking and motion blur artefacts through the optimisation of experimental parameters. For that purpose, we have coupled a numerical approach relying on tomography simulation with vibrating particles with known and ideal 3D geometry (micro-spheres or fibres) with experimental campaigns. These were carried out on soft composites, imaged in synchrotron X-ray beamlines while oscillating up to 400 Hz, thanks to a custom-developed vibromechanical device. This approach yields homogeneous angular sampling of projections and gives reliable predictions of image quality degradation due to motion blur. By overcoming several technical and scientific barriers limiting the feasibility and reproducibility of such investigations, we provide guidelines to enhance gated-CT 4D imaging for the analysis of heterogeneous, high-frequency oscillating materials. [ABSTRACT FROM AUTHOR]

COVID has affected the performance of small-scale businesses in Lagos positively and negatively. The purpose of this research work is to investigate the impact of COVID on the performance of small-scale businesses in Lagos. One of the contributions of this research work is the bringing together, the concepts of the impact of COVID, and Nigeria's economy in the context of small-scale businesses in Lagos. 24 research cluster groups from academia's and Lagos small-scale business organizations partook in the quantitative research study that made use of focus group discussions for data collection that was analyzed through content analysis and reliability of inter-rater. The finding is that COVID has impacted small-scale businesses in Lagos negatively. This research work provides recommendations for Lagos state government and the federal government of Nigeria. [ABSTRACT FROM AUTHOR]

Implementation of evidence-based interventions (EBIs) in under-resourced schools serving ethnic and racial minority youth is challenged by intervention, individuals', and setting characteristics. Engaging community members in equitable partnership improves implementation outcomes and provides resources (e.g., workforce capacity, funding), but can be time intensive. Rapid and rigorous approaches for improving the implementation of EBIs in under-resourced schools is necessary to address youth mental health needs. In this paper, we describe a 6-week internship program for high school students (n = 8) that used community-based participatory research (CBPR) and user-centered design (UCD) principles to adapt a cognitive behavioral skills curriculum. We assessed the process by categorizing barriers discussed and addressed using the Consolidated Framework for Implementation Research, and qualitatively exploring youth perceptions of the adapted curriculum. Barriers included intervention complexity, intervention design, communication, and resource availability. Adaptations focused on design simplification (e.g., renaming the curriculum TEB: Thoughts, Emotions, Behaviors) and dissemination strategies (e.g., creating social media content). Thematic analysis of intern interviews revealed the adapted curriculum as appropriate for students, helpful, and broadly applicable. Interns also expressed ownership over the curriculum and provided recommendations for future implementation. Overall, our study suggests the following: (1) CBPR and UCD can be integrated to adapt EBIs for racial and ethnic minority youth in school settings; (2) UCD principles can expedite the adaptation process; (3) design participation fosters a sense of ownership; (4) youth involvement in the design process can spur support from other stakeholder groups including policymakers; and (5) engaging youth as co-creators requires financial and human resources. [ABSTRACT FROM AUTHOR]

This article explores the use of islands as tools of geographical and intellectual containment - or what we call “islanding” - in the scientific and policy literature about gene drive technologies in conservation. In the first part of the article, we explore the narrative of contained gene drive use on islands and discuss how it juggles notions of localness and localization of gene drives and their (test) releases. We question the possibility and narrative of containing the spread of gene drives technologically or geographically, and argue that the gene drives for conservation literature strategically combines contradictory and reductive understandings of islands and containment. The second part of the article is devoted to reflection on nonlocal concerns about gene drives and the possibility of local gene drive decisions. We argue that attempts to legitimize local gene drives through local decision-making evade normative concerns about their nonlocalizability and risk instrumentalizing local communities for nonlocal agendas. Our overarching conceptual aim is therefore to open up a domain of thinking around the possibility of demarcation in our world – of our political, normative decisions, and of our reality – and to argue for the vital importance of reflection on this possibility in technological decision-making. [ABSTRACT FROM AUTHOR]

Introduction: Self-criticism and subjective emptiness have been identified as possible transdiagnostic risk factors for various mental disorders. Guided by minority stress theory, this cross-sectional study revealed different latent profiles of these two theorized risk factors and their associations with childhood gender nonconformity and self-stigma. Methods: A sample including 366 gay and bisexual men was employed in the current study, using latent profile analysis to explore their self-criticism and subjective emptiness vulnerability profiles. Results: Results of latent profile analysis identified four possible profiles based on distinct combinations of self-criticism and subjective emptiness, including low-, mild-, moderate-, and high-level risk profiles. In addition, the profiles were differentially associated with self-stigma and childhood gender nonconformity. Policy Implications: We hope our findings will encourage Chinese gay and bisexual men to actively deal with underlying psychological processes and attempt to reconcile with themselves. We also suggest that the relevant personnel engaged in psychological counseling, psychotherapy, psychological services, and clinicians should pay attention to their clients' self-criticism and subjective emptiness experience when assisting these populations. Policies should support and encourage society to jointly create a relaxed and inclusive environment. [ABSTRACT FROM AUTHOR]

Regardless of the type, extracorporeal membrane oxygenation (ECMO) requires the use of large intravascular cannulas and results in multiple abnormalities including non-physiologic blood flow, hemodynamic perturbation, rapid changes in blood oxygen and carbon dioxide levels, coagulation abnormalities, and a significant systemic inflammatory response. Among other sequelae, neurologic complications are an important source of mortality and long-term morbidity. The frequency of neurologic complications varies and is likely underreported due to the high mortality rate. Neurologic complications in patients supported by ECMO include ischemic and hemorrhagic stroke, hypoxic brain injury, intracranial hemorrhage, and brain death. In addition to the disease process that necessitates ECMO, cannulation strategies and physiologic disturbances influence neurologic outcomes in this high-risk population. For example, the overall documented rate of neurologic complications in the venovenous ECMO population is lower, but a higher rate of intracranial hemorrhage exists. Meanwhile, in the venoarterial ECMO population, ischemia and global hypoperfusion seem to compose a higher percentage of neurologic complications. In what follows, the literature is reviewed to discuss the pathophysiology, incidence, risk factors, and outcomes related to short-term neurologic complications in patients supported by ECMO. [ABSTRACT FROM AUTHOR]

Water pollution is a pressing global concern, with per- and polyfluoroalkyl substances (PFAS) being considered as "forever contaminants." Among them, perfluorooctanesulfonic acid (PFOS) has received significant attention for its adverse effects on human health and aquatic ecosystems. This study aimed to design an innovative adsorbent for effective PFOS removal with exceptional water stability, improving its cost-performance trade-off. The current work simultaneously improved the stability of water of Cu-based metal–organic framework (CMOF) and increased its PFOS removal capacity by modifying it with amine-functionalized SiO2 nanoparticles (AF-CMOF). AF-CMOF presented a lower specific surface area of 999 m2 g−1 compared to CMOF with a surface area of 1098 m2 g−1. AF-CMOF showed remarkable PFOS uptake performance of 670 mg/g compared to the performance of the Cu-based MOF which exhibited a PFOS uptake capacity of only 22 mg/g. The most suitable pH for PFOS removal using both adsorbents was determined to be 3. In addition, AF-CMOF demonstrated excellent water stability, retaining its structural integrity even after seven days of water contact, while CMOF structure collapsed rapidly after four days of water exposure. Moreover, the study identified the significant pH influence on the PFOS uptake process, with electrostatic interactions between protonated amine functionalities and PFOS molecules identified as the dominant mechanism. The study's findings present the potential of synthesized adsorbent as a superior candidate for PFOS uptake and contribute to the development of effective water treatment technologies. [ABSTRACT FROM AUTHOR]

Recognition of the high prevalence of trauma exposure and posttraumatic stress reactions among young offenders has led to calls for a shift toward the implementation of trauma-informed approaches in juvenile justice systems. However, meeting the clinical needs of youth in this population not only requires comprehending the profound effects of polyvictimization, developmental trauma, and complex PTSD but also an appreciation of the ways in which traumatic experiences and posttraumatic sequela intersect with diverse youth identities, including ethnicity and race, gender, sexual minority status, developmental stage, and gang involvement, which in turn may affect engagement in treatment. Ethical, legal, and clinical challenges specific to providing services in the context of the juvenile justice system also must be considered, including complications related to confidentiality, protection from self-incrimination, threats to psychological safety, and the potential for secondary traumatic stress among service providers delivering trauma-focused interventions in forensic contexts. This paper reviews those issues and goes on to describe the existing evidence base for interventions for posttraumatic reactions among justice-involved youth, as well as its limitations, and points toward future directions for research and clinical developments that could to expand the precision and reach of mental health services for youth at risk or already involved in the legal system. [ABSTRACT FROM AUTHOR]

Adolescents involved in the juvenile legal system (JLS) report high rates of traumatic event exposure and posttraumatic stress symptoms. Due to such elevated rates of exposure to potentially traumatic events and posttraumatic stress, it has been recommended that adolescents involved in the JLS receive access to trauma-focused screening upon each contact with the system. Implementing trauma-focused screening within the JLS raises several considerations for systems who have yet to implement this evidence-based practice. This article applies the Exploration, Preparation, Implementation, and Sustainment (EPIS) framework to discuss important decisions and potential ethical issues that arise when trauma-focused screening is implemented within the JLS, from the initial planning stages, to implementation, to long-term sustainment. This article discusses decision points that JLS leadership and administrators must consider when determining whether to implement trauma-focused screening or in the initial stages of implementation, as well as several practical suggestions for individual practitioners who work within the JLS, including clinical and non-clinical staff. [ABSTRACT FROM AUTHOR]

Soil organic carbon (SOC) is a critical variable used to determine the carbon balance. However, large uncertainties arise when predicting the SOC stock in soil profiles in Chinese grasslands, especially on desert rangelands. Recent studies have shown that desert ecosystems may be potential carbon sinks under global climate change. Because of the high spatial heterogeneity, time-consuming sampling methods, and difficult acquisition process, the relationships the SOC storage and distribution have with driving factors in desert rangelands remain poorly understood. Here, we investigated and developed an SOC database from 3162 soil samples (collected at depths of 0–10 cm and 10–20 cm) across 527 sites, as well as the climate conditions, vegetation types, and edaphic factors associated with the sampling sites in the desert rangelands of northern Xinjiang, north-west China. This study aims to determine the SOC magnitude and drivers in desert rangelands. Our findings demonstrate that the SOC and SOC density (SOCD) were 0.05–37.13 g·kg−1 and 19.23–9740.62 g·m−2, respectively, with average values of 6.81 ± 5.31 g·kg−1 and 1670.38 ± 1202.52 g·m−2, respectively. The spatial distributions of SOC and SOCD all showed gradually decreasing trends from south-west to north-east. High-SOC areas were mainly distributed in the piedmont lowlands of the Ili valley, while low-SOC regions were mainly concentrated in the north-west area of Altay. The redundancy analysis results revealed that all environmental factors accounted for approximately 37.6% of the spatial variability in SOC; climate factors, vegetation factors, and soil properties explained 15.0%, 1.7%, and 12.3%, respectively. The structural equation model (SEM) further indicated that evapotranspiration, average annual precipitation, and the SWC were the dominant factors affecting SOC accumulation, mainly through direct effects, although indirect effects were also delivered by the vegetation factors. Taken together, the results obtained herein updated the SOC data pool available for desert rangelands and clarified the main driving factors of SOC variations. This study provided supporting data for the sustainable use and management of desert rangelands and the global ecosystem carbon budget. [ABSTRACT FROM AUTHOR]

Background: The COVID-19 pandemic resulted in unprecedented changes to cancer care in many countries, impacting cancer patients' lives in numerous ways. This study examines the impact of changes in cancer care on patient's health-related quality of life (HRQL), which is a key outcome in cancer care. The study aims to estimate patients' self-reported HRQL before and during the pandemic and identify predictive factors for their physical and mental wellbeing. Method: The study employed the large-scale Outpatient Cancer Care (OCC) Patient Experience Survey, including the Veterans RAND 12-Item Health Survey, to evaluate cancer patients' experiences and HRQL before (January to May 2020) and during the COVID-19 pandemic (May to July 2021). Paired t-tests were conducted to compare differences in Physical Component Scores (PCS) and Mental Component Scores (MCS) before and during the pandemic. Multivariable linear regressions were employed to investigate the factors (sociodemographic, clinical, and patient-reported experience) influencing PCS and MCS during the pandemic. Results: PCS decreased significantly during the pandemic, while MCS remained stable. Lower PCS contributors included older age, more telehealth visits, self-reported hospitalization, and a longer time since the last cancer diagnosis. Higher PCS was associated with urban residence, higher MCS during the pandemic, and perceived active Healthcare Provider (HCP) involvement. For MCS, lower scores related to female gender and more telehealth visits, while higher scores were associated with being white, higher education, high MCS before the pandemic, and perceived active HCP involvement. Conclusion: The OCC Patient Experience Survey provides a unique patient level data set measuring HRQL pre- and post- the onset of the COVID-19 pandemic. The study highlights challenges faced by cancer patients during the pandemic, with a significant reduction in PCS. However, the stability in MCS suggests effective coping mechanisms. Sociodemographic, clinical, and telehealth-related variables play a complex role in shaping both PCS and MCS. Perceived HCP involvement emerges as a crucial factor correlating with higher PCS and MCS. Navigating the post-pandemic era necessitates interventions fortifying patient-provider relationships, optimizing healthcare support systems, such as telehealth services, and prioritizing mental-well-being given its impact on both PCS and MCS. Plain English summary: This study delves into the impact of changes in cancer care and COVID-19 measures on the well-being of cancer patients. As cancer care shifted during the pandemic, we aimed to understand how it affected patients' quality of life. The study discovered that physical well-being significantly decreased, especially for older patients with more telehealth visits, while mental well-being remained stable, indicating effective coping strategies. Factors like the number of telehealth visits, healthcare provider engagement, and pre-pandemic mental health status played pivotal roles in shaping the mental and physical well-being of cancer patients during the COVID-19 pandemic. This study emphasizes the challenges faced by cancer patients during the pandemic and highlights the importance of supportive interventions for patient-provider relationships and prioritizing mental-wellbeing in the post-pandemic era. [ABSTRACT FROM AUTHOR]

Fiber optic localized surface plasmon resonance (LSPR) sensors have become an effective tool in refractive index (RI) detection for biomedical applications because of their high sensitivity. However, using conventional optical fiber has caused limitations in implanting the sensor in the body. This research presents the design and construction of a new type of polymer-based LSPR sensors to address this issue. Also, finite element method (FEM) is used to design the sensor and test it theoretically. The proposed polymer optical fiber (POF) based on citrate is biocompatible, flexible, and degradable, with a rate of 22% and 27 over 12 days. The step RI structure utilizes two polymers for light transmission: poly (octamethylene maleate citrate) (POMC) as the core and poly (octamethylene citrate) (POC) as the cladding. The POF core and cladding diameters and lengths are 700 µm, 1400 µm, and 7 cm, respectively. The coupling efficiency of light to the POF was enhanced using a microsphere fiber optic tip. The obtained results show that the light coupling efficiency increased to 77.8%. Plasma surface treatment was used to immobilize gold nanoparticles (AuNPs) on the tip of the POF, as a LSPR-POF sensor. Adsorption kinetics was measured based on the pseudo-first-order model to determine the efficiency of immobilizing AuNPs, in which the adsorption rate constant (k) was obtained be 8.6 × 10–3 min−1. The RI sensitivity of the sensor in the range from 1.3332 to 1.3604 RIU was obtained as 7778%/RIU, and the sensitivity was enhanced ~ 5 times to the previous RI POF sensors. These results are in good agreement with theory and computer simulation. It promises a highly sensitive and label-free detection biosensor for point-of-care applications such as neurosciences. [ABSTRACT FROM AUTHOR]

This study aimed to determine the sequence type (ST) of Bartonella henselae infecting small Indian mongooses from Saint Kitts via multi-locus sequence typing (MLST). This investigation used stored EDTA blood (n = 22) samples from mongooses previously identified as positive for B. henselae. Chocolate agar plates were enriched with Bartonella alpha-Proteobacteria growth medium (BAPGM) to culture and isolate Bartonella from the blood samples. To perform MLST, DNA was extracted and purified from isolates followed by amplification by conventional PCR (300–500 bp) for eight genes (16S rDNA, batR, gltA, groEL, ftsZ, nlpD, ribC, and rpoB). Bartonella henselae STs were deposited in the PubMLST repository. Out of 22 B. henselae-positive blood samples, isolates were obtained from 12 mongooses (54.5%; 12/22). Each mongoose was infected with one ST. The studied mongoose population was infected with sequence types ST2, ST3, ST8, and a novel ST represented by ST38. Bartonella henselae ST2, ST3 and ST8 infecting mongooses are known to circulate in humans and cats, with ST2 and ST8 associated with Cat Scratch Disease (bartonellosis) in humans. The results presented herein denote the circulation of B. henselae STs with zoonotic potential in mongooses with risk of B. henselae transmission to humans. [ABSTRACT FROM AUTHOR]

Long COVID, now unmistakably identified as a syndromic entity encompassing a complex spectrum of symptoms, demands immediate resolution of its elusive pathogenic underpinnings. The intricate interplay of diverse factors presents a complex puzzle, difficult to resolve, and thus poses a substantial challenge. As instances of long COVID manifest by repeated infections of SARS-CoV-2 and genetic predisposition, a detailed understanding in this regard is needed. This endeavor is a comprehensive exploration and analysis of the cascading pathogenetic events driven by viral persistence and replication. Beyond its morbidity, long COVID, more disabling than fatal, exacts one of the most substantial tolls on public health in contemporary times, with the potential to cripple national economies. The paper introduces a unified theory of long COVID, detailing a novel pathophysiological framework that interlinks persistent SARS-CoV-2 infection, autoimmunity, and systemic vascular pathology. We posit a model where viral reservoirs, immune dysregulation, and genetic predispositions converge to perpetuate disease. It challenges prevailing hypotheses with new evidence, suggesting innovative diagnostic and therapeutic approaches. The paper aims to shift the paradigm in long COVID research by providing an integrative perspective that encapsulates the multifaceted nature of the condition. We explain the immunological mechanisms, hypercoagulability states, and viral reservoirs in the skull that feed NeuroCOVID in patients with long COVID. Also, this study hints toward a patient approach and how to prioritize treatment sequences in long COVID patients in hospitals and clinics. [ABSTRACT FROM AUTHOR]

Memory for truth and falsity has recently been investigated from the perspective of the dual-recollection theory, showing better context and target recollection for truth than falsity. In this paper, we examine whether these memory effects obtained for true statements are similar to the value effect, whereby true statements are given higher priority in encoding. For this purpose, we implemented value-directed remembering (VDR) into the conjoint-recognition paradigm. In our first experiment, the primary goal was to verify how VDR influences the processes defined by dual-recollection theory. At study, prioritized/important items were linked to higher numerical values (e.g., 10), while unimportant ones had lower values (e.g., 1). At test, the participants' task was to recognize whether a particular sentence was important, unimportant, or new. We found that both context and target recollection were better for important items. In the second experiment, the main goal was to study the combined effects of importance and veracity on memory. In the between-subjects design, participants were monetarily rewarded for memorizing true or false sentences. The results demonstrated differences in the ability to prioritize truth over falsity. Specifically, we found a substantial increase in context recollection for prioritized true information but not for prioritized false information. Moreover, we found higher context recollection for true than false sentences in the true-prioritized condition, but not in the false-prioritized condition. These results indicated that people are able to prioritize true information better than false, and suggested that memory for truth may be a special case of the value effect. [ABSTRACT FROM AUTHOR]

The Density Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm is one of the most popular and effective density-based clustering algorithms at present. Although it can effectively identify clusters and noise points of arbitrary shapes, it is very difficult to efficiently address the tasks with large scale data. The time complexity of the DBSCAN is O (n 2) where its main computation time lies in ε -neighbor range query, which becomes the bottleneck of DBSCAN performance. To solve this problem, we propose a simple fast DBSCAN algorithm, called bh-DBSCAN, using a bi-directional HNSW index structure to improve the efficiency of DBSCAN by reducing redundant ε -neighbor range queries. Specifically, we first distinguish a point's property (core point or border point). Next, we apply the filtNoise algorithm to filter the noise points that without core points in n e i g h b o r x . Finally, we utilized the MergeCore algorithm to merge the cluster of border points in it's core neighbor points. The experimental results show that our proposed algorithm could greatly improve the clustering efficiency without losing much accuracy based on the datasets tested. [ABSTRACT FROM AUTHOR]

Software verification aims to prove that a program satisfies some given properties for all its possible executions. Software evolved incredibly fast during the last century, exposing several challenges to this scientific discipline. The goal of the "Challenges of Software Verification Symposium" is to monitor the state-of-the-art in this field. In this article, we will present the evolution of software from its inception in the 1940s to today's applications, how this exposed new challenges to software verification, and what this discipline achieved. We will then discuss how this chapter covers most of the current open challenges, the possible future software developments, and what challenges this will raise in software verification. [ABSTRACT FROM AUTHOR]

Background: The Brain Oxygen Optimization in Severe Traumatic Brain Injury Phase II randomized controlled trial used a tier-based management protocol based on brain tissue oxygen (PbtO2) and intracranial pressure (ICP) monitoring to reduce brain tissue hypoxia after severe traumatic brain injury. We performed a secondary analysis to explore the relationship between brain tissue hypoxia, blood pressure (BP), and interventions to improve cerebral perfusion pressure (CPP). We hypothesized that BP management below the lower limit of autoregulation would lead to cerebral hypoperfusion and brain tissue hypoxia that could be improved with hemodynamic augmentation. Methods: Of the 119 patients enrolled in the Brain Oxygen Optimization in Severe Traumatic Brain Injury Phase II trial, 55 patients had simultaneous recordings of arterial BP, ICP, and PbtO2. Autoregulatory function was measured by interrogating changes in ICP and PbtO2 in response to fluctuations in CPP using time-correlation analysis. The resulting autoregulatory indices (pressure reactivity index and oxygen reactivity index) were used to identify the "optimal" CPP and limits of autoregulation for each patient. Autoregulatory function and percent time with CPP outside personalized limits of autoregulation were calculated before, during, and after all interventions directed to optimize CPP. Results: Individualized limits of autoregulation were computed in 55 patients (mean age 38 years, mean monitoring time 92 h). We identified 35 episodes of brain tissue hypoxia (PbtO2 < 20 mm Hg) treated with CPP augmentation. Following each intervention, mean CPP increased from 73 ± 14 mm Hg to 79 ± 17 mm Hg (p = 0.15), and mean PbtO2 improved from 18.4 ± 5.6 mm Hg to 21.9 ± 5.6 mm Hg (p = 0.01), whereas autoregulatory function trended toward improvement (oxygen reactivity index 0.42 vs. 0.37, p = 0.14; pressure reactivity index 0.25 vs. 0.21, p = 0.2). Although optimal CPP and limits remained relatively unchanged, there was a significant decrease in the percent time with CPP below the lower limit of autoregulation in the 60 min after compared with before an intervention (11% vs. 23%, p = 0.05). Conclusions: Our analysis suggests that brain tissue hypoxia is associated with cerebral hypoperfusion characterized by increased time with CPP below the lower limit of autoregulation. Interventions to increase CPP appear to improve autoregulation. Further studies are needed to validate the importance of autoregulation as a modifiable variable with the potential to improve outcomes. [ABSTRACT FROM AUTHOR]

Flow has been defined as a state of full immersion that may emerge when the skills of a person match the challenge of an activity. It is a special case of being on task, as during flow, keeping focused on the task feels effortless. Most experimental investigations of the neural or physiological correlates of flow contrast conditions with different levels of challenge. Yet comparing different levels of challenge that are too distant may trigger states where the participant is off task, such as boredom or frustration. Thus, it remains unclear whether previously observed differences ascribed to flow may rather reflect differences in how much participants were on task—trying their best—across the contrasted conditions. To remedy this, we introduce a method to manipulate flow by contrasting two video game play conditions at personalized levels of difficulty calibrated such that participants similarly tried their best in both conditions. Across three experiments (> 90 participants), higher flow was robustly reported in our high-flow than in our low-flow condition (mean effect size d = 1.31). Cardiac, respiratory, and skin conductance measures confirmed the known difference between a period of rest and the two on-task conditions of high and low flow, but failed to distinguish between these latter two. In light of the conflicting findings regarding the physiological correlates of flow, we discuss the importance of ensuring a low-flow baseline condition that maintains participants on task, and propose that the present method provides a methodological advance toward that goal. [ABSTRACT FROM AUTHOR]

The global CO2 concentration is predicted to reach 700 µmol·mol−1 by the end of this century. Phoebe bournei (Hemsl.) Yang is a precious timber species and is listed as a national secondary protection plant in China. P. bournei seedlings show obvious photosynthetic decline when grown long-term under an elevated CO2 concentration (eCO2, EC). This decline can be alleviated by high nitrate or ammonium applications. However, the underlying mechanisms have not yet been elucidated. We performed transcriptomic and proteomic analyses of P. bournei of seedlings grown under an ambient CO2 concentration (AC) and applied with either a moderate level of nitrate (N), a high level of nitrate (hN), or a moderate level of ammonium (A) and compared them with those of seedlings grown under eCO2 (i.e., AC_N vs EC_N, AC_hN vs EC_hN, AC_A vs EC_A) to identify differentially expressed genes (DEGs) and differentially expressed proteins (DEPs). We identified 4528 (AC_N vs EC_N), 1378 (AC_hN vs EC_hN), and 252 (AC_A vs EC_A) DEGs and 230, 514, and 234 DEPs, respectively, of which 59 specific genes and 21 specific proteins were related to the regulation of photosynthesis by nitrogen under eCO2. A combined transcriptomic and proteomic analysis identified 7 correlation-DEGs-DEPs genes. These correlation-DEGs-DEPs genes revealed crucial pathways involved in glyoxylate and dicarboxylate metabolism and nitrogen metabolism. The rbcS and glnA correlation-DEGs-DEPs genes were enriched in these two metabolisms. We propose that the rbcS and glnA correlation-DEGs-DEPs genes play an important role in photosynthetic decline and nitrogen regulation. High nitrate or ammonium applications alleviated the downregulation of glnA and rbcS and, hence, alleviated photosynthetic decline. The results of this study provide directions for the screening of germplasm resources and molecular breeding of P. bournei, which is tolerant to elevated CO2 concentrations. [ABSTRACT FROM AUTHOR]

Meta-analyses of mindfulness-based interventions (MBIs) in schools consistently show small, positive effects, but the field remains dominated by studies with low methodological rigour. Recent large randomised controlled trials in adolescents demonstrate poor traction in this age group together with some adverse effects, creating a crossroads for their future. In their recent commentary on the null effects of the MYRIAD trial (Kuyken et al., 2022), Strohmaier and Bailey (2023) postulated that making mindfulness practice available within the school day may increase the dosage and benefits of MBIs for adolescents, and called for funders to direct efforts at developing and testing this approach. We agree that identifying ways to increase dose beyond weekly classroom lessons is important, but explore whether this suggestion is practical or developmentally appropriate for secondary school settings. Our commentary broadens to group together other large and moderately large RCTs that have also shown disappointing results in adolescents, and presents seven barriers that together may be compounding the lack of effect in this age group. We offer alternative suggestions for future school-based research and delivery of MBIs. [ABSTRACT FROM AUTHOR]