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Benefiting from self-attention mechanism and convolutional operation, numerous lightweight Transformer-based single image super-resolution (SISR) approaches have made considerable breakthroughs in performance. Nevertheless, higher computational costs of self-attention and parametric overheads of convolution still limit the deployment of these methods on low-budget devices. To tackle the above issues, based on the sequentially cascaded attention and convolution-cooperative blocks (ACCBs), we propose an attention and convolution-cooperative network (ACCNet) for lightweight image super-resolution (SR). Specifically, in each ACCB, to alleviate the computational burden of self-attention mechanism, we propose a multi-group window self-attention (MGWSA), which achieves self-attention calculation on different groups of features via different window sizes. To implement a lightweight convolutional operation that assists self-attention for local feature extraction, we propose a residual blueprint separable convolution (RBSC), which combines the advantages of efficient convolution and residual learning. Additionally, an enhanced multi-layer perceptron (EMLP) is designed to strengthen the representation of features spatially. The contrast-aware channel attention (CCA) is introduced to exploit the beneficial interdependency among channels. Compared to other lightweight state-of-the-art SR methods, the better trade-off between model complexity and performance in our ACCNet is demonstrated by extensive experiments. [ABSTRACT FROM AUTHOR]

This study focused on the investigation of the edge effect of diamond films deposited by microwave plasma chemical vapor deposition. Substrate bulge height is a factor that affects the edge effect, and it was used to simulate plasma and guide the diamond-film deposition experiments. Finite-element software COMSOL Multiphysics was used to construct a multiphysics (electromagnetic, plasma, and fluid heat transfer fields) coupling model based on electron collision reaction. Raman spectroscopy and scanning electron microscopy were performed to characterize the experimental growth and validate the model. The simulation results reflected the experimental trends observed. Plasma discharge at the edge of the substrate accelerated due to the increase in Δh (Δh = 0–3 mm), and the values of electron density (ne), molar concentration of H (CH), and molar concentration of CH 3 (C CH 3 ) doubled at the edge (for the special concave sample with Δh = −1 mm, the active chemical groups exhibited a decreased molar concentration at the edge of the substrate). At Δh = 0–3 mm, a high diamond growth rate and a large diamond grain size were observed at the edge of the substrate, and their values increased with Δh. The uniformity of film thickness decreased with Δh. The Raman spectra of all samples revealed the first-order characteristic peak of diamond near 1332 cm−1. When Δh = −1 mm, tensile stress occurred in all regions of the film. When Δh = 1–3 mm, all areas in the film exhibited compressive stress. [ABSTRACT FROM AUTHOR]

Purpose: Selective tibial neurotomy (STN) is a surgical procedure for treating spastic equinovarus foot. Hyperselective neurectomy (HSN) of tibial nerve is a modified STN procedure, which was rarely discussed. This study aimed to describe the branching patterns of the tibial nerve and propose an optimal surgical incision of HSN for treatment of spastic equinovarus foot. Methods: Sixteen lower limbs were dissected to determine the various branching patterns of the tibial nerve and categorized according to these branching patterns. The mean distances from the nerve entry points to the tip of femur's medial epicondyle were measured, as well as their percentage to the overall length of the leg. The surgical incision was designed according to the range of these nerve entry points. Results: The tibial nerve sent out proximal and distal motor branches based on their position relative to the soleus muscle's tendinous arch. For proximal motor branches, the branches innervating the medial gastrocnemius, lateral gastrocnemius and proximal soleus were categorized into types I (9/16), II (5/16) and III (2/16). Measurements from the medial epicondyle to the nerve entry points into the medial gastrocnemius, lateral gastrocnemius and proximal soleus ranged from 14 to 33 mm (4–9% of leg length), 22–45 mm (6–12%) and 35–81 mm (10–22%), respectively. Distal motor branches including the distal soleus, posterior tibialis, flexor digitorum longus and flexor hallucis longus, were classified as types A (8/14), B (4/14) and C (2/14), with the distances from their respective terminal points to the medial epicondyle were 67–137 mm (19–39%), 74–125 mm (20–35%), 116–243 mm (33–69%) and 125–272 mm (35–77%). Conclusions: The motor branches of tibial nerve were classified into two groups and each subdivided into three types. Detailed location parameters may serve as an anatomical basis for designing incision of HSN. [ABSTRACT FROM AUTHOR]

Enhancing the quality and safety of exported agricultural products and improving export competitiveness is the key to establishing enhanced competitive advantages in agricultural products, developing a trade powerhouse and realising high-quality development of agriculture. This paper uses the data of Chinese listed companies and Chinese Customs from 2007 to 2016 to discuss the effect and mechanism of digital transformation of enterprises on the quality and safety level of export agricultural products by using the staggered differential method. The study shows that (1) Enterprise digital transformation effectively improves the quality and safety of exported agricultural products, and this result holds after endogeneity, placebo and multiple robustness tests; (2) Heterogeneity analyses reveal that the quality and safety effect of enterprise digital transformation is greater for exporting to developed countries' markets, non-state-owned enterprises and enterprises in the eastern region, in addition to bulk agricultural products and consumer-oriented agricultural products; (3) Mechanism analyses shows that enterprise digital transformation raises the quality and safety of exported agricultural products through technological innovation, product tracing, information sharing and quality assurance effects. [ABSTRACT FROM AUTHOR]

The low-frequency oscillation (LFO) has occurred in the train–network system due to the introduction of the power electronics of the trains. The modeling and analyzing method in current researches based on electrified railway unilateral power supply system are not suitable for the LFO analysis in a bilateral power supply system, where the trains are supplied by two traction substations. In this work, based on the single-input and single-output impedance model of China CRH5 trains, the node admittance matrices of the train–network system both in unilateral and bilateral power supply modes are established, including three-phase power grid, traction transformers and traction network. Then the modal analysis is used to study the oscillation modes and propagation characteristics of the unilateral and bilateral power supply systems. Moreover, the influence of the equivalent inductance of the power grid, the length of the transmission line, and the length of the traction network are analyzed on the critical oscillation mode of the bilateral power supply system. Finally, the theoretical analysis results are verified by the time-domain simulation model in MATLAB/Simulink. [ABSTRACT FROM AUTHOR]

A key strategy for designing environmental barrier coatings is to incorporate multiple rare-earth (RE) components into β- and γ-RE2Si2O7 to achieve multifunctional performance optimization. However, the polymorphic phase presents significant challenges for the design of multicomponent RE disilicates. Here, employing decision fusion, a machine learning (ML) method is crafted to identify multicomponent RE disilicates, showcasing notable accuracy in prediction. The well-trained ML models evaluated the phase formation capability of 117 (RE10.25RE20.25Yb0.25Lu0.25)2Si2O7 and (RE11/6RE21/6RE31/6Gd1/6Yb1/6Lu1/6)2Si2O7, which are unreported in experiments and validated by first-principles calculations. Utilizing model visualization, essential factors governing the formation of (RE10.25RE20.25Yb0.25Lu0.25)2Si2O7 are pinpointed, including the average radius of RE3+ and variations in different RE3+ combinations. On the other hand, (RE11/6RE21/6RE31/6Gd1/6Yb1/6Lu1/6)2Si2O7 must take into account the average mass and the electronegativity deviation of RE3+. This work combines material-oriented ML methods with formation mechanisms of multicomponent RE disilicates, enabling the efficient design of superior materials with exceptional properties for the application of environmental barrier coatings. [ABSTRACT FROM AUTHOR]

Background: Central obesity is a risk factor for chronic kidney disease (CKD). However, the exact correlation between the cardiometabolic index (CMI), an indicator of central obesity, and CKD remains unclear. Here, we aimed to investigate the correlation between the CMI and CKD in the general American population. Methods: This cross-sectional study involved 64,313 members of the general population (≥ 20 years of age) with data in the National Health and Nutrition Examination Survey (NHANES) 1999–2020. The individuals were grouped into three categories by CMI tertile: T1 group (n = 7,029), T2 group (n = 7,356), and T3 group (n = 7,380). Logistic regression analysis was performed, with NHANES recommended weights, to assess the association between the CMI and CKD. Results: A total of 21,765 participants were included; the overall prevalence of CKD was 12.2%. From the low to the high CMI tertile, the prevalence of CKD increased from 8.9% to 16.0% (P < 0.001). After full adjustment for confounders, the higher tertile of CMI (OR: 1.08, 95% CI: 1.03 − 1.13, P = 0.002) had the higher risk of CKD. Compared with the T1 group, the groups with higher CMI levels had a higher CKD risk (T2: OR: 1.01, 95%CI: 0.87–1.18, P = 0.812; T3: OR: 1.22, 95%CI: 1.05–1.43, P = 0.013). Conclusions: Higher CMI was independently associated with higher CKD risk in the general population. [ABSTRACT FROM AUTHOR]

Linear ubiquitination catalyzed by HOIL-1-interacting protein (HOIP), the key component of the linear ubiquitination assembly complex, plays fundamental roles in tissue homeostasis by executing domain-specific regulatory functions. However, a proteome-wide analysis of the domain-specific interactome of HOIP across tissues is lacking. Here, we present a comprehensive mass spectrometry-based interactome profiling of four HOIP domains in nine mouse tissues. The interaction dataset provides a high-quality HOIP interactome resource with an average of approximately 90 interactors for each bait per tissue. HOIP tissue interactome presents a systematic understanding of linear ubiquitination functions in each tissue and also shows associations of tissue functions to genetic diseases. HOIP domain interactome characterizes a set of previously undefined linear ubiquitinated substrates and elucidates the cross-talk among HOIP domains in physiological and pathological processes. Moreover, we show that linear ubiquitination of Integrin-linked protein kinase (ILK) decreases focal adhesion formation and promotes the detachment of Shigella flexneri-infected cells. Meanwhile, Hoip deficiency decreases the linear ubiquitination of Smad ubiquitination regulatory factor 1 (SMURF1) and enhances its E3 activity, finally causing a reduced bone mass phenotype in mice. Overall, our work expands the knowledge of HOIP-interacting proteins and provides a platform for further discovery of linear ubiquitination functions in tissue homeostasis. Authors perform an in vivo mass spectrometry-based interactome analysis of HOIL-1-interacting protein, a key component of linear ubiquitination assembly complex. [ABSTRACT FROM AUTHOR]

Purpose: CUDC-907 is a promising dual-target inhibitor of the HDAC and PI3K signaling pathways, with demonstrated therapeutic effects in a range of malignant tumors. However, its potential application in ovarian cancer (OC) has not been fully explored yet. In this study, we sought to investigate the efficacy of CUDC-907 in treating OC, both in vitro and in vivo. Methods: Here, we examined the correlation between PI3K or HDAC expression and the prognosis of OC patients using the GEPIA database. RNA-Seq analysis was performed on OC cells treated with CUDC-907.To assess various cellular processes, including proliferation, migration, invasion, apoptosis, and cell cycle, we performed a series of assays, including the CCK8, EDU, wound healing, cell invasion, and flow cytometry assays. Real-time quantitative PCR and western blotting were performed to measure the expressions of target genes. Additionally, we utilized the SKOV3 xenograft tumor model to investigate the inhibitory effects of CUDC-907 on tumor growth in vivo. Results: Bioinformatics analyses revealed that up-regulated HDAC and PI3K were significantly correlated with patients' poor survival in OC. In vivo and in vitro experiments have demonstrated that CUDC-907 could inhibit the proliferation of OC cells by inhibiting the PI3K and HDAC pathways to down-regulate the expression of c-Myc, and induce cell apoptosis by inhibiting the PI3K/AKT/Bcl-2 pathway, and up-regulate p21 to induce G2 /M phase arrest. Conclusion: Our results showed that CUDC-907 had powerful anti-tumor effects on OC, which could provide a theoretical and experimental basis for the application of CUDC-907 in the therapy of OC. [ABSTRACT FROM AUTHOR]

Coherent beam combination offers a solution to the challenges associated with the power handling capacity of individual fibres, however, the combined intensity profile strongly depends on the relative phase of each fibre. Optimal combination necessitates precise control over the phase of each fibre channel, however, determining the required phase compensations is challenging because phase information is typically not available. Additionally, the presence of continuously varying phase noise in fibre laser systems means that a single-step and high-speed correction process is required. In this work, we use a spatial light modulator to demonstrate coherent combination in a seven-beam system. Deep learning is used to identify the relative phase offsets for each beam directly from the combined intensity pattern, allowing real-time correction. Furthermore, we demonstrate that the deep learning agent can calculate the phase corrections needed to achieve user-specified target intensity profiles thus simultaneously achieving both beam combination and beam shaping. [ABSTRACT FROM AUTHOR]

Electrochemical carbon dioxide reduction reaction using sustainable energy is a promising approach of synthesizing chemicals and fuels, yet is highly energy intensive. The oxygen evolution reaction is particularly problematic, which is kinetically sluggish and causes anodic carbon loss. In this context, we couple CO2 electrolysis with hydrogen oxidation reaction in a single electrochemical cell. A Ni(OH)2/NiOOH mediator is used to fully suppress the anodic carbon loss and hydrogen oxidation catalyst poisoning by migrated reaction products. This cell is highly flexible in producing either gaseous (CO) or soluble (formate) products with high selectivity (up to 95.3%) and stability (>100 h) at voltages below 0.9 V (50 mA cm−2). Importantly, thanks to the "transferred" oxygen evolution reaction to a water electrolyzer with thermodynamically and kinetically favored reaction conditions, the total polarization loss and energy consumption of our H2-integrated CO2 reduction reaction, including those for hydrogen generation, are reduced up to 22% and 42%, respectively. This work demonstrates the opportunity of combining CO2 electrolysis with the hydrogen economy, paving the way to the possible integration of various emerging energy conversion and storage approaches for improved energy/cost effectiveness. Electrochemical CO2 reduction is a promising method of producing sustainable chemicals and fuels, yet is highly energy intensive. Here, the authors couple CO2 electrolysis with hydrogen oxidation using a Ni(OH)2/NiOOH auxiliary electrode to enhance energy efficiency. [ABSTRACT FROM AUTHOR]

Objectives: To develop a model that can assist in the diagnosis and prediction of prognosis for head and neck squamous cell carcinoma (HNSCC). Materials and methods: Data from TCGA and GEO databases were used to generate normalized gene expression data. Consensus Cluster Plus was used for cluster analysis and the relationship between angiogenesis-associated gene (AAG) expression patterns, clinical characteristics and survival was examined. Support vector machine (SVM) and least absolute shrinkage and selection operator (LASSO) analyzes and multiple logistic regression analyzes were performed to determine the diagnostic model, and a prognostic nomogram was constructed using univariate and multivariate Cox regression analyses. ESTIMATE, XCELL, TIMER, QUANTISEQ, MCPCOUNTER, EPIC, CIBERSORT-ABS, CIBERSORT algorithms were used to assess the immune microenvironment of HNSCC patients. In addition, gene set enrichment analysis, treatment sensitivity analysis, and AAGs mutation studies were performed. Finally, we also performed immunohistochemistry (IHC) staining in the tissue samples. Results: We classified HNSCC patients into subtypes based on differences in AAG expression from TCGA and GEO databases. There are differences in clinical features, TME, and immune-related gene expression between two subgroups. We constructed a HNSCC diagnostic model based on nine AAGs, which has good sensitivity and specificity. After further screening, we constructed a prognostic risk signature for HNSCC based on six AAGs. The constructed risk score had a good independent prognostic significance, and it was further constructed into a prognostic nomogram together with age and stage. Different prognostic risk groups have differences in immune microenvironment, drug sensitivity, gene enrichment and gene mutation. Conclusion: We have constructed a diagnostic and prognostic model for HNSCC based on AAG, which has good performance. The constructed prognostic risk score is closely related to tumor immune microenvironment and immunotherapy response. [ABSTRACT FROM AUTHOR]

Fungal pathogens typically use secreted effector proteins to suppress host immune activators to facilitate invasion. However, there is rarely evidence supporting the idea that fungal secretory proteins contribute to pathogenesis by transactivating host genes that suppress defense. We previously found that pathogen Magnaporthe oryzae induces rice Bsr-d1 to facilitate infection and hypothesized that a fungal effector mediates this induction. Here, we report that MoSPAB1 secreted by M. oryzae directly binds to the Bsr-d1 promoter to induce its expression, facilitating pathogenesis. Amino acids 103-123 of MoSPAB1 are required for its binding to the Bsr-d1 promoter. Both MoSPAB1 and rice MYBS1 compete for binding to the Bsr-d1 promoter to regulate Bsr-d1 expression. Furthermore, MoSPAB1 homologues are highly conserved among fungi. In particular, Colletotrichum fructicola CfSPAB1 and Colletotrichum sublineola CsSPAB1 activate kiwifruit AcBsr-d1 and sorghum SbBsr-d1 respectively, to facilitate pathogenesis. Taken together, our findings reveal a conserved module that may be widely utilized by fungi to enhance pathogenesis. Magnaporthe oryzae effector MoSPAB1 enters rice nuclei to bind to the promoter of the immunity-brake gene Bsr-d1 and activates its expression by competing with rice MYBS1, which constitute a conserved module that facilitates fungal pathogenesis. [ABSTRACT FROM AUTHOR]

Osmotic energy generation with reverse electrodialysis through membranes provides a worldwide free energy resource. Photo-driven proton transport in photosynthesis supplies basal energy for plants and living organisms on the planet. Here, we utilized aramid nanofiber (ANF) semiconductor-based membranes to enable light-driven proton transport for osmotic energy generation. Under unilateral illumination, the light-driven proton transport system converted light energy into electrical energy and showed wavelength- and intensity-dependent transmembrane potentials and currents. Interestingly, the synergistic effects of simultaneous illumination and pressure provided a five-fold increase in the voltage and a three-fold increase in the current relative to pressure alone. Density functional theory calculations and spectroscopic measurements demonstrated that the ANF and photoinduced electrons enabled proton transport during illumination and generated a transmembrane potential and current. The light-driven proton transport system supports the development of devices with flexible and stable ANF membranes. Osmotic energy generation, using aramid nanofiber (ANF) semiconductor membranes for light-driven proton transport, displayed wavelength and intensity-dependent potential and current under unilateral illumination. The simultaneous application of illumination and pressure led to a five-fold voltage increase and a three-fold current increase. Density functional theory calculations and spectroscopic measurements confirmed ANF's role in photoinduced proton transport. This research has significant implications for developing flexible, stable ANF membrane-based energy devices. [ABSTRACT FROM AUTHOR]

Background: Major depressive and social anxiety disorders have a high comorbidity rate and similar cognitive patterns. However, their unique and shared neuroanatomical characteristics have not been fully identified. Methods: Voxel-based morphometric studies comparing gray matter volume between patients with major depressive disorder/social anxiety disorder and healthy controls were searched using 4 electronic databases from the inception to March 2022. Stereotactic data were extracted and subsequently tested for convergence and differences using activation likelihood estimation. In addition, based on the result of the meta-analysis, behavioral analysis was performed to assess the functional roles of the regions affected by major depressive disorder and/or social anxiety disorder. Results: In total, 34 studies on major depressive disorder with 2873 participants, and 10 studies on social anxiety disorder with 1004 subjects were included. Gray matter volume conjunction analysis showed that the right parahippocampal gyrus region, especially the amygdala, was smaller in patients compared to healthy controls. The contrast analysis of major depressive disorder and social anxiety disorder revealed lower gray matter volume in the right lentiform nucleus and medial frontal gyrus in social anxiety disorder and lower gray matter volume in the left parahippocampal gyrus in major depressive disorder. Behavioral analysis showed that regions with lower gray matter volume in social anxiety disorder are strongly associated with negative emotional processes. Conclusions: The shared and unique patterns of gray matter volume abnormalities in patients with major depressive and social anxiety disorder may be linked to the underlying neuropathogenesis of these mental illnesses and provide potential biomarkers. PROSPERO registration number: CRD42021277546. [ABSTRACT FROM AUTHOR]

Triterpenoids, as the main active ingredient of Ganoderma lucidum fermented extract, exert multiple pharmacological activities, including immunomodulatory properties. Our study aimed to reveal the pharmacological effects and potential mechanisms of Ganoderic acid C2 (GAC) against cyclophosphamide (CY)-associated immunosuppression. Target genes were collected from several public databases, including the DisGeNET, Comparative Toxicogenomics Database, GeneCards, and PharmMapper. STRING database was used to construct the protein–protein interaction of network. Subsequently, molecular docking was carried out to visualize the protein-GAC interactions. Experimental validations, including ELISA and qRT-PCR were performed to confirm the pharmacological activities of GAC on CY-induced immunosuppression model. A total of 56 GAC-related targets were identified to be closely associated with CY-induced immunosuppression. Enrichment analyses results revealed that these targets were mainly involved in immune and inflammatory response-related pathways. STAT3 and TNF were identified as the core targets of GAC. Molecular docking indicated that GAC combined well with STAT3 and TNF protein. In addition, animal experiments indicated that GAC improved immunity as well as STAT3 and TNF genes expression in CY-induced immunosuppression, which further verified the prediction through bioinformatics analysis and molecular docking. We successfully revealed the potential therapeutics mechanisms underlying the effect of GAC against CY-induced immunosuppression based on the combination of bioinformatics analysis, molecular docking, and animal experiments. Our findings lay a theoretical foundation for the in-depth development and utilization of Ganoderma lucidum fermentation product in the future, and also provide theoretical guidance for the development of innovative drugs that assist in improving immunity. [ABSTRACT FROM AUTHOR]

Improving the quality of agricultural exports and increasing value–added trade is crucial for shaping new competitive advantages of agricultural exports, building a strong trading nation and achieving high–quality agricultural development. This study constructed a multi–time point difference–in–differences model to empirically test the effect and mechanism of the mutual recognition of geographical indications (GIs) between China and the European Union (EU) on the quality upgrading of China's exported agricultural products from 2000 to 2016 based on theoretical analysis of the effect mechanism of GIs on agricultural product exports. The study determined that mutual GI recognition between China and the EU has effectively improved the quality of agricultural exports, with a greater effect on upgrading the quality of products from countries with high GIs endowment, quality frontier and large and medium–sized enterprises and labour–intensive products. The mechanism analysis revealed that mutual GI recognition between China and the EU can improve the quality of agricultural exports through specialisation agglomeration and cost-saving effects on the supply side and domestic demand upgrading and product recognition effects on the demand side. This study has important implications for further enriching quality improvement theory regarding agricultural products and presents a new approach for enhancing the quality improvement path of exported agricultural products that lack supply–side resources. [ABSTRACT FROM AUTHOR]

The apoptotic cells were observed and calculated in T24/5637 cells treated with "amiRNA -catenin" (C-F) or "amiRNA HIF-1 " (C-F) induced by doxycycline using Hoechst 33,258 staining assay. The relative rate of cell migration was calculated in T24 (A) and 5637 (B) cells treated with "amiRNA -catenin" or "amiRNA HIF-1 " induced by doxycycline using wound-healing assay. Author Correction: Synthetic Tet-inducible artificial microRNAs targeting -catenin or HIF-1 inhibit malignant phenotypes of bladder cancer cells T24 and 5637. [Extracted from the article]

(Zr0.6336Cu0.1452Ni0.1012Al0.12)97.4Er2.6 bulk metallic glass (BMG) was prepared by copper mold suction casting, and the effects of sub-Tg annealing and cryogenic thermal cycle (CTC) treatment on its microstructure and properties were investigated. Although the specimens after annealing and CTC treatment did not have significant changes in the amorphous structure, the treated specimens exhibited excellent compressive plastic strain of 16.44 and 19.08%, representing an enhancement of 22.59 and 42.28% compared with the as-cast specimens. The change in plasticity was closely related to the arrangement of atoms and the content and distribution of the free volume caused by sub-Tg annealing and CTC treatment. The corrosion resistance of the specimen was further optimized after sub-Tg annealing and CTC treatment and gradually increased with the increase of annealing temperature and the CTC times. This work demonstrated that appropriate annealing temperature and CTC conditions could be used as an effective and controllable way to modulate the mechanical properties of BMGs. [ABSTRACT FROM AUTHOR]

Arrestins have pivotal roles in regulating G protein-coupled receptor (GPCR) signalling by desensitizing G protein activation and mediating receptor internalization1,2. It has been proposed that the arrestin binds to the receptor in two different conformations, ‘tail’ and ‘core’, which were suggested to govern distinct processes of receptor signalling and trafficking3,4. However, little structural information is available for the tail engagement of the arrestins. Here we report two structures of the glucagon receptor (GCGR) bound to β-arrestin 1 (βarr1) in glucagon-bound and ligand-free states. These structures reveal a receptor tail-engaged binding mode of βarr1 with many unique features, to our knowledge, not previously observed. Helix VIII, instead of the receptor core, has a major role in accommodating βarr1 by forming extensive interactions with the central crest of βarr1. The tail-binding pose is further defined by a close proximity between the βarr1 C-edge and the receptor helical bundle, and stabilized by a phosphoinositide derivative that bridges βarr1 with helices I and VIII of GCGR. Lacking any contact with the arrestin, the receptor core is in an inactive state and loosely binds to glucagon. Further functional studies suggest that the tail conformation of GCGR–βarr governs βarr recruitment at the plasma membrane and endocytosis of GCGR, and provides a molecular basis for the receptor forming a super-complex simultaneously with G protein and βarr to promote sustained signalling within endosomes. These findings extend our knowledge about the arrestin-mediated modulation of GPCR functionalities.Structures of the glucagon receptor bound to β-arrestin 1 are reported, providing further information about the arrestin-mediated modulation of G protein-coupled receptors. [ABSTRACT FROM AUTHOR]

Purpose: We conducted this study to investigate the efficacy, safety, and clinical value of postoperative compression in preventing seroma formation, relieving acute pain, and improving QoL after groin hernia repair. Methods: This multi-center, prospective, observational real-world study was conducted from March 1, 2022, to August 31, 2022. The study was completed in 53 hospitals in 25 provinces in China. A total of 497 patients who underwent groin hernia repair were enrolled. All patients used a compression device to compress the operative region after surgery. The primary outcome was seroma incidence 1 month after surgery. Secondary outcomes included postoperative acute pain and QoL. Results: A total of 497 patients [median (IQR) age 55 (41–67) years, 456 (91.8%) male] were enrolled, of whom 454 underwent laparoscopic groin hernia repair and 43 open hernia repair. The follow-up rate was 98.4% 1 month after surgery. Seroma incidence was 7.2% (35 of 489 patients) overall, lower than reported by previous research. No significant differences were found between the two groups (P > 0.05). VAS scores after compression were significantly lower than before compression overall and in both groups (P < 0.001). The laparoscopic group showed a high level of QoL compared with the open group, but there was no significant difference between the two groups (P > 0.05). CCS score correlated positively with VAS score. Conclusion: Postoperative compression, to a certain extent, can reduce seroma incidence, relieve postoperative acute pain, and improve QoL after groin hernia repair. Further large-scale randomized controlled studies are warranted to determine long-term outcomes. [ABSTRACT FROM AUTHOR]

This document is a correction notice for an article titled "Theophylline controllable RNAi-based genetic switches regulate expression of lncRNA TINCR and malignant phenotypes in bladder cancer cells" published in Scientific Reports. The correction addresses errors in Figure 6 of the article, specifically in the grouping of data points within the '5637 ON-NC' subgroup. The corrected figure and accompanying legend are provided. The article discusses the effects of the ON device on the proliferation and apoptosis of bladder cancer cells in vitro, demonstrating that the ON device can activate the inhibition of cell proliferation and promote apoptosis when the lncRNA TINCR is silenced. The authors of the article are Zhicong Chen, Yuchen Liu, Anbang He, and others. [Extracted from the article]

Five choline ionic liquids were selected to evaluate their toxicity to green alga (Chlorella vulgaris) and Daphnia magna. Their influence on chlorophyll content, respiration rate, antioxidase activity, and malondialdehyde content of Chlorella vulgaris and the acute and chronic toxicity of Daphnia magna were investigated. The studied ionic liquids slightly inhibited the algal growth with the median effective concentrations (EC50) > 1900 mg/L. Chlorophyll contents, respiration rate, and protein contents of the alga after 96 h of exposure decreased with the higher concentrations of the ionic liquids, while superoxide dismutase and catalase activity and malondialdehyde content increased firstly and then decreased. The acute toxicity of Daphnia magna after 48 h of exposure (indicated by the median effective concentrations, EC50) was 200.44 and 184.50 mg/L for choline acetate and choline D-lactate, respectively, which was above the toxicity of other ionic liquids. The studied ionic liquids presented low toxicity to Chlorella vulgaris and Daphnia magna. [ABSTRACT FROM AUTHOR]

Purpose of Review: Despite progress in the treatment of pediatric B-cell acute lymphoblastic leukemia (ALL) and PH + ALL, fewer advancements have been for older adults with PH-negative B-cell ALL. Treatment of this population is mired by higher incidence of poor risk biologic features, increased incidence of medical comorbidities, and higher rates of treatment-related mortality (TRM). Here, we review the difficulties in managing elderly patients with PH-negative ALL. Recent Findings: The development of novel agents has brought additional tools to the armamentarium of drugs and has changed the landscape of treatment. More recent clinical trials and future clinical trials focus on blinatumomab, inotuzomab ozogamicin (IO), and/or chimeric antigen receptor T-cell (CAR-T) either alone or integrated with dose-reduced chemotherapy regimens. Summary: The introduction of novel agents/therapies and incorporation into our current treatment paradigms may finally offer an avenue to improve the dismal outcomes seen in this population. [ABSTRACT FROM AUTHOR]

Bilayer graphene (BLG) is intriguing for its unique properties and potential applications in electronics, photonics, and mechanics. However, the chemical vapor deposition synthesis of large-area high-quality bilayer graphene on Cu is suffering from a low growth rate and limited bilayer coverage. Herein, we demonstrate the fast synthesis of meter-sized bilayer graphene film on commercial polycrystalline Cu foils by introducing trace CO2 during high-temperature growth. Continuous bilayer graphene with a high ratio of AB-stacking structure can be obtained within 20 min, which exhibits enhanced mechanical strength, uniform transmittance, and low sheet resistance in large area. Moreover, 96 and 100% AB-stacking structures were achieved in bilayer graphene grown on single-crystal Cu(111) foil and ultraflat single-crystal Cu(111)/sapphire substrates, respectively. The AB-stacking bilayer graphene exhibits tunable bandgap and performs well in photodetection. This work provides important insights into the growth mechanism and the mass production of large-area high-quality BLG on Cu. Bilayer graphene (BLG) is promising for optoelectronic applications due to its tunable bandgap, but its large-area growth on Cu substrates is still challenging. Here, the authors demonstrate the fast synthesis of high-coverage meter-scale BLG on commercial Cu foils by introducing CO2 during the growth. [ABSTRACT FROM AUTHOR]

Lamellar graphene oxide (GO) membranes have gained substantial interest for molecular separation processes. However, GO membranes have shown inefficient separation performance levels due to their possession of sufficient functional groups that lead to swelling under applied hydraulic pressure. Herein, we demonstrate a highly stable and ultrafast filtrable graphene oxide-boron nitride (GOBN) hybrid membrane by incorporating boron nitride (BN) nanosheets into a GO membrane to restrict swelling and provide efficient hydraulic pressure separation characteristics. This new heterostructure retains the GOBN membrane microstructure and provides more nanochannels around the incorporation sites due to the small size of BN nanosheets; this phenomenon increases the permeance to 1310 Lm−2h−1bar−1, which is nearly six times higher than that of the pure GO membrane, with a high rejection reaching 99.2% for aqueous organic dyes. More importantly, the GOBN hybrid membrane shows an impressive permeance and dye molecule rejection performance characteristic across a range of organic solvents, such as methanol, ethanol, and isopropyl alcohol; the performance characteristics are better than those for the GO membrane. Our GOBN membrane with a stable microstructure opens opportunities for developing a high-performance multiple solvent nanofiltration membrane that surpasses the permeability-selectivity trade-off. [ABSTRACT FROM AUTHOR]

The conductivity of the electrolyte and the kinetics of Li+ inside lithium-ion batteries (LIBs) will decrease at low temperatures, which may promote the formation of lithium dendrite. The growing of lithium dendrites will penetrate the separator, and cause the internal short circuits and thermal runaway of cells. Thus, battery preheating is essential to improve the safety of LIBs. To investigate the temperature changes of battery during discharging and preheating at low temperatures, the electro-thermal model and the preheating model of LIBs at low temperature are established and verified based on the second-order equivalent circuit model. The internal resistance of battery decreases with the increase of temperature. Moreover, a battery module with polyimide flexible heating film is proposed, and the heating films are arranged on both sides of the battery symmetrically. When the power of heating films is 1 W, 3 W, and 5 W, it takes 395 s, 190 s and 126 s to preheat the battery temperature from − 10°C to 25°C, respectively. Additionally, different heating powers can be arranged in the heating process to reduce the heating time and temperature difference of battery. The research in this study contributes to the preheating of LIBs in cold regions, and has certain reference value. [ABSTRACT FROM AUTHOR]

We show that the K-moduli spaces of log Fano pairs (P 3 , c S) where S is a quartic surface interpolate between the GIT moduli space of quartic surfaces and the Baily–Borel compactification of moduli of quartic K3 surfaces as c varies in the interval (0, 1). We completely describe the wall crossings of these K-moduli spaces. As the main application, we verify Laza–O'Grady's prediction on the Hassett–Keel–Looijenga program for quartic K3 surfaces. We also obtain the K-moduli compactification of quartic double solids, and classify all Gorenstein canonical Fano degenerations of P 3 . [ABSTRACT FROM AUTHOR]

BeiDou Satellite-based Augmentation System (BDSBAS) has come into the trial operation stage since July, 2020. To evaluate the characteristic of the augmentation message in BDSBAS-B1C signal, the effectiveness of the message content was firstly analyzed, and then the validity of the broadcasting strategy was estimated. Finally, the accuracy of the user equivalent ranging error (UERE) and the single frequency positioning error with different correction parameters in BDSBAS-B1C message was evaluated. Based on the above analysis, the effectiveness of the augmentation message was preliminarily verified with the results showing that: (1) the BDSBAS-B1C message type, information content and update interval have basically met the international standard; (2) the accuracy of the UERE obtained with the augmentation message had an obvious improvement in contrast to that of the UERE obtained with the usual navigation message of the GPS satellites, and the ionospheric delay was one of the important factors which affected the accuracy of the UERE; (3) the positioning accuracy obtained with the augmentation message was also improved, and the improvement was more obvious in the service areas with high availability of the ionospheric parameters. [ABSTRACT FROM AUTHOR]

The effect of antipsychotic medications is critical for the long-term outcome of symptoms and functions during first-episode psychosis (FEP). However, how brain functions respond to the antipsychotic treatment in the early stage of psychosis and its underlying neural mechanisms remain unclear. In this study, we explored the cross-sectional and longitudinal changes of regional homogeneity (ReHo), whole-brain functional connectivity, and network topological properties via resting-state functional magnetic resonance images. Thirty-two drug-naïve FEP patients and 30 matched healthy volunteers (HV) were included, where 23 patients were re-visited with effective responses after two months of antipsychotic treatment. Compared to HV, drug-naive patients demonstrated significantly different patterns of functional connectivity involving the right thalamus. These functional alterations mainly involved decreased ReHo, increased nodal efficiency in the right thalamus, and increased thalamic-sensorimotor-frontoparietal connectivity. In the follow-up analysis, patients after treatment showed reduced ReHo and nodal clustering in visual networks, as well as disturbances of visual-somatomotor and hippocampus-superior frontal gyrus connectivity. The longitudinal changes of ReHo in the visual cortex were associated with an improvement in general psychotic symptoms. This study provides new evidence regarding alterations in brain function linked to schizophrenia onset and affected by antipsychotic medications. Moreover, our results demonstrated that the functional alterations at baseline were not fully modulated by antipsychotic medications, suggesting that antipsychotic medications may reduce psychotic symptoms but limit the effects in regions involved in disease pathophysiology. [ABSTRACT FROM AUTHOR]

SRC is the first identified oncogene, and its aberrant activation has been implicated as a driving event in tumor initiation and progression. However, its role in cancer stemness regulation and the underlying regulatory mechanism are still elusive. Here, we identified a YAP1 tyrosine phosphorylation-dependent YAP1-KLF5 oncogenic module, as the key downstream mediator of SRC kinase regulating cancer stemness and metastasis in triple-negative breast cancer (TNBC). SRC was overexpressed in TNBC patient tissues and its expression level was highly correlated with the tumor malignancy. SRC activation induced, while inhibition of SRC kinase reduced the cancer stemness, tumor cell growth and metastasis in vitro and in vivo. Transcriptomic and proteomic analysis revealed that SRC-mediated YAP1 tyrosine phosphorylation induced its interaction with Kruppel-like factor 5 (KLF5) to form a YAP1/TEAD-KLF5 complex in TNBC cells. YAP1-KLF5 association further promoted TEAD-mediated transcriptional program independently of canonical Hippo kinases, which eventually gave rise to the enhanced cancer stemness and metastasis. Disruption of YAP1-KLF5 module in TNBC cells dramatically attenuated the SRC-induced cancer stemness and metastasis in vitro and in vivo. Accordingly, co-upregulations of SRC and YAP1-KLF5 module in TNBC tissues were significantly positively correlated with the tumor malignance. Altogether, our work presents a novel tyrosine phosphorylation-dependent YAP1-KLF5 oncogenic module governing SRC-induced cancer stemness and metastasis in TNBC. Therefore, targeting YAP1/KLF5-mediated transcription may provide a promising strategy for TNBC treatment with SRC aberrantly activation. [ABSTRACT FROM AUTHOR]

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We prove that every smooth Fano threefold from the family N o ̲ 2.8 is K-stable. Such a Fano threefold is a double cover of the blow-up of P 3 at one point branched along an anti-canonical divisor. [ABSTRACT FROM AUTHOR]

Physicians write clinical notes with abbreviations and shorthand that are difficult to decipher. Abbreviations can be clinical jargon (writing "HIT" for "heparin induced thrombocytopenia"), ambiguous terms that require expertise to disambiguate (using "MS" for "multiple sclerosis" or "mental status"), or domain-specific vernacular ("cb" for "complicated by"). Here we train machine learning models on public web data to decode such text by replacing abbreviations with their meanings. We report a single translation model that simultaneously detects and expands thousands of abbreviations in real clinical notes with accuracies ranging from 92.1%-97.1% on multiple external test datasets. The model equals or exceeds the performance of board-certified physicians (97.6% vs 88.7% total accuracy). Our results demonstrate a general method to contextually decipher abbreviations and shorthand that is built without any privacy-compromising data. Patient notes contain shorthand and abbreviations that may be jargon or clinical vernacular. Here the authors train large machine learning models on public web data to decode such text by replacing abbreviations with their meanings. [ABSTRACT FROM AUTHOR]

Little is known about the impact of the COVID-19 pandemic on medical students. We examined medical students’ burnout, career regret, and medical school experiences from before to during the pandemic, and differences between students from marginalized and nonmarginalized groups. We analyzed data from 2019 to 2022 Association of American Medical Colleges Year Two Questionnaires (N = 52,152) and Graduation Questionnaires (N = 66,795). Given large samples, we focused on effect sizes versus statistical significance. All effects of study year were less than small (η2 < .01) indicating minimal differences in medical students’ burnout, career regret, and school experiences from before (2019) to during (2020–2022) the pandemic. Interactions between study year and demographic characteristics (gender, race-ethnicity, sexual orientation) were less than small; thus, students from marginalized groups were not affected more by the pandemic than others. Across study years, women reported more exhaustion and discrimination than men. Black students reported more discrimination than students from other racial-ethnic groups; bisexual students reported more discrimination than heterosexual students (all ds > 0.20). Differences between students from marginalized and nonmarginalized groups were bigger than the effects of the pandemic, suggesting a need for system-level interventions to foster inclusion in medical education. [ABSTRACT FROM AUTHOR]

Methanogens and anaerobic methane-oxidizing archaea (ANME) are important players in the global carbon cycle. Methyl-coenzyme M reductase (MCR) is a key enzyme in methane metabolism, catalyzing the last step in methanogenesis and the first step in anaerobic methane oxidation. Divergent mcr and mcr-like genes have recently been identified in uncultured archaeal lineages. However, the assembly and biochemistry of MCRs from uncultured archaea remain largely unknown. Here we present an approach to study MCRs from uncultured archaea by heterologous expression in a methanogen, Methanococcus maripaludis. Promoter, operon structure, and temperature were important determinants for MCR production. Both recombinant methanococcal and ANME-2 MCR assembled with the host MCR forming hybrid complexes, whereas tested ANME-1 MCR and ethyl-coenzyme M reductase only formed homogenous complexes. Together with structural modeling, this suggests that ANME-2 and methanogen MCRs are structurally similar and their reaction directions are likely regulated by thermodynamics rather than intrinsic structural differences. A heterologous protein expression platform in a culturable methanogen Methanococcus maripaludis is developed to study the methyl-coenzyme M reductase from uncultured archaea. [ABSTRACT FROM AUTHOR]

Purpose: This study aimed to evaluate the safety and efficacy of chimeric antigen receptor (CAR) disialoganglioside 2 (GD2)-specific (4SCAR-GD2) T cells for treatment of refractory and/or recurrent neuroblastoma (NB) in pediatric patients. Experimental design: A phase I clinical study using 4SCAR-GD2 T cells for the treatment of NB in pediatric patients was conducted. This study was registered at www.clinicaltrials.gov (NCT02765243). A lentiviral CAR with the signaling domains of CD28/4-1BB/CD3ζ-iCasp9 was transduced into activated T cells. The response to 4SCAR-GD2 T-cell treatment, and 4SCAR-GD2 T-cell expansion and persistence in patients were evaluated. Toxicities were determined based on the National Cancer Institute Common Terminology Criteria for Adverse Events (CTCAE) v4.03. Results: Twelve patients were enrolled and finally ten patients were included in this clinical trial which started from January 1, 2016, to August 1, 2017. These patients had progressive disease (PD) before CAR T-cell infusion. After 4SCAR-GD2 T-cell treatment, 6 (6/10) had stable disease (SD) at 6 months, and 4 (4/10) remained SD at 1 year and alive after 3–4 years of follow-up. Six patients died due to disease progression by the end of July 1, 2020. The median overall survival (OS) time was 25 months (95% CI, 0.00–59.43), and the median progression-free survival (PFS) time was 8 months (95% CI, 0.25–15.75). Grade 3 or 4 hematological toxicities were the common adverse events frequently occurred after fludarabine and cyclophosphamide (Flu/cy) chemotherapy. Grade 1–2 toxicities such as cytokine release syndrome (CRS) and neuropathic pain were common, but were transient and mild. Conclusions: The 4SCAR-GD2 T-cell therapy demonstrated antitumor effect and manageable toxicities, indicating its potential to benefit children with refractory and/or recurrent NB. [ABSTRACT FROM AUTHOR]

Supramolecular self-assembly of μm-to-mm sized components is essential to construct complex supramolecular systems. However, the selective assembly to form designated structures at this length scale is challenging because the short-ranged molecular recognition could hardly direct the assembly of macroscopic components. Here we demonstrate a self-sorting mechanism to automatically identify the surface chemistry of μm-to-mm components (A: polycations; B: polyanions) based on the A-B attraction and the A-A repulsion, which is realized by the additivity and the competence between long-ranged magnetic/capillary forces, respectively. Mechanistic studies of the correlation between the magnetic/capillary forces and the interactive distance have revealed the energy landscape of each assembly pattern to support the self-sorting results. By applying this mechanism, the assembly yield of ABA trimers has been increased from 30%~40% under conventional conditions to 100% with self-sorting. Moreover, we have demonstrated rapid and spontaneous self-assembly of advanced chain-like structures with alternate surface chemistry. The selective assembly of μm-to-mm sized components is challenging because of the short-ranged molecular recognition. Here, the authors demonstrate a self-sorting mechanism to automatically identify the surface chemistry via additivity and competence between long-ranged magnetic/capillary forces. [ABSTRACT FROM AUTHOR]

In this paper, we propose a multimodal flexible sensory interface for interactively teaching soft robots to perform skilled locomotion using bare human hands. First, we develop a flexible bimodal smart skin (FBSS) based on triboelectric nanogenerator and liquid metal sensing that can perform simultaneous tactile and touchless sensing and distinguish these two modes in real time. With the FBSS, soft robots can react on their own to tactile and touchless stimuli. We then propose a distance control method that enabled humans to teach soft robots movements via bare hand-eye coordination. The results showed that participants can effectively teach a self-reacting soft continuum manipulator complex motions in three-dimensional space through a "shifting sensors and teaching" method within just a few minutes. The soft manipulator can repeat the human-taught motions and replay them at different speeds. Finally, we demonstrate that humans can easily teach the soft manipulator to complete specific tasks such as completing a pen-and-paper maze, taking a throat swab, and crossing a barrier to grasp an object. We envision that this user-friendly, non-programmable teaching method based on flexible multimodal sensory interfaces could broadly expand the domains in which humans interact with and utilize soft robots. Soft robots are challenging to model and program. Non-specialists face non-negligible obstacles when working with soft robots to perform tasks. Here, the authors propose a method to interactively teach soft robots complex motions through flexible touchless and tactile multimodal sensors. [ABSTRACT FROM AUTHOR]

Hippo signaling restricts tissue growth by inhibiting the transcriptional effector YAP. Here we uncover a role of Hippo signaling and a tumor suppressor function of YAP in estrogen receptor positive (ER+) breast cancer. We find that inhibition of Hippo/MST1/2 or activation of YAP blocks the ERα transcriptional program and ER+ breast cancer growth. Mechanistically, the Hippo pathway transcription factor TEAD physically interacts with ERα to increase its promoter/enhancer occupancy whereas YAP inhibits ERα/TEAD interaction, decreases ERα occupancy on its target promoters/enhancers, and promotes ERα degradation by the proteasome. Furthermore, YAP inhibits hormone-independent transcription of ERα gene (ESR1). Consistently, high levels of YAP correlate with good prognosis of ER+ breast cancer patients. Finally, we find that pharmacological inhibition of Hippo/MST1/2 impeded tumor growth driven by hormone therapy resistant ERα mutants, suggesting that targeting the Hippo-YAP-TEAD signaling axis could be a potential therapeutical strategy to overcome endocrine therapy resistance conferred by ERα mutants. Recent studies have reported that oncoprotein YAP can function as tumour suppressor in certain contexts. Here the authors show that inhibition of Hippo signalling or YAP activation blocks ERα transcriptional program and ER + breast cancer growth and mechanistically this is through YAP interfering with TEAD-ERα signalling axis. [ABSTRACT FROM AUTHOR]

Background: Whether to preserve the uterine round ligament during laparoscopic inguinal hernia repair in women is controversial. In this study, we aimed to compare outcomes of uterine round ligament preservation versus transection during such surgery and to explore the impact and long-term outcomes of transecting the round ligament. Methods: The study cohort comprised 419 women who had undergone laparoscopic inguinal hernia repair in Beijing Chaoyang Hospital and Qilu Hospital from January 2013 to January 2020; 393 (93.8%) of whom were successfully followed up. Patient characteristics and technical details of the operative procedure were collected and analyzed retrospectively. Early and late postoperative follow-up data, complications, especially symptoms related to retroflexed uterus, and fertility outcomes, were collected by a single follow-up nurse who was blinded to the operative procedure. Results: There were 218 women (239 sides) in the uterine round ligament preservation group and 175 (182 sides) in the transection group. The patients in the preservation group were younger (45.9 vs. 53.6 years, p = 0.000), and had lower American Society of Anesthesiologists scores (p = 0.000). The median follow-up times in the preservation and transection groups were 41.8 ± 24.2 and 42.7 ± 24.6 months, respectively (p = 0.692). Compared with the transection group, the preservation group had longer operative times for repair of both primary and recurrent hernias. Intraoperative bleeding, length of hospital stay, development of seromas, recurrence rate, incidence of postoperative pain at the first and third postoperative months, and time of last outpatient visit were similar in the two groups. There were more premenopausal patients in the preservation group; however, we found no evidence that transection of the round ligament affected subsequent pregnancy or childbirth. Moreover, we identified no differences in dyspareunia, dysmenorrhea, chronic pelvic pain, or uterine prolapse. Conclusion: Transection of the round ligament during laparoscopic inguinal hernia repair in women does not increase the incidence of dyspareunia, dysmenorrhea, chronic pelvic pain, or uterine prolapse, whereas it has the advantage of reducing the operation time. [ABSTRACT FROM AUTHOR]

A safety assessment was conducted for Beishan region of Gansu, China, the results show that Se-79 and Cs-135 are the key nuclides released in the near field between 1000 and 4000 years after the closure of the repository. After 4000 years, Tc-99, Np-237 and U-235 are the key nuclides released in the near field. Within 5000 years after the closure of the repository, Se-79 is the key nuclide released from the dominant water fractures, and its peak release rate is 1 × 105 mol/m3, peak time 4000 a. At 20,000 years after the closure of the repository, Cs-135 is the key nuclide, and its peak release rate is 5 × 106 mol/m3, with a peak time of 100,000 a. [ABSTRACT FROM AUTHOR]

Chemical vapor deposition (CVD)-grown graphene films on Cu foils, exhibiting fine scalability and high quality, are still suffering from the adverse impact of surface contamination, i.e., amorphous carbon. Despite the recent successful preparation of superclean graphene through Cu-vapor-assisted reactions, the formation mechanism of amorphous carbon remains unclear, especially with regard to the functions of substrates. Herein, we have found that the crystallographic orientations of underlying metal substrates would determine the cleanness of graphene in such a way that slower diffusion of active carbon species on as-formed graphene-Cu(100) surface is the key factor that suppresses the formation of contamination. The facile synthesis of clean graphene is achieved on the meter-sized Cu(100) that is transformed from the polycrystalline Cu foils. Furthermore, a clean surface of graphene on Cu(100) ensures the reduction of transfer-related polymer residues, and enhanced optical and electrical performance, which allows for versatile applications of graphene in biosensors, functioning as flexible transparent electrodes. This work would offer a promising material platform for the fundamental investigation and create new opportunities for the advanced applications of high-quality graphene films. [ABSTRACT FROM AUTHOR]

A key challenge in designing intelligent artificial gene circuits is generating flexible connections between arbitrary components and directly coupling them with endogenous signaling pathways. The CRISPR signal conductor based on conditionally inducible artificial transcriptional regulators can link classic cellular protein signals with targeted gene expression, but there are still problems with multiple signal processing and gene delivery. With the discovery and characterization of new Cas systems and long noncoding RNA (lncRNA) functional motifs, and because of the compatibility of guide RNA with noncoding RNA elements at multiple sites, it is increasingly possible to solve these problems. In this study, we developed CRISPR signal conductor version 2.0 by integrating various lncRNA functional motifs into different parts of the crRNA in the CRISPR-dCasΦ system. This system can directly regulate the expression of target genes by recruiting cellular endogenous transcription factors and efficiently sense a variety of protein signals that are not detected by a classical synthetic system. The new system solved the problems of background leakage and insensitive signaling responses and enabled the construction of logic gates with as many as six input signals, which can be used to specifically target cancer cells. By rewiring endogenous signaling networks, we further demonstrated the effectiveness and biosafety of this system for in vivo cancer gene therapy. [ABSTRACT FROM AUTHOR]

For two graphs G 1 and G 2 , the star-critical Ramsey number r ∗ (G 1 , G 2) is the minimum integer k such that any red/blue edge-coloring of K r - 1 ⊔ K 1 , k contains a red copy of G 1 or a blue copy of G 2 , where r is the classical Ramsey number R (G 1 , G 2) and K r - 1 ⊔ K 1 , k is the graph obtained from a K r - 1 and an additional vertex v by joining v to k vertices of K r - 1 . Let C n denote a cycle of order n and W m a wheel of order m + 1 . Hook (2010) proved that r ∗ (C n , W 3) = 2 n for n ≥ 5 . In this paper, it is shown that r ∗ (C n , W m) = 2 n for m odd, n ≥ m ≥ 5 and n ≥ 60 . [ABSTRACT FROM AUTHOR]

We establish an algebraic approach to prove the properness of moduli spaces of K-polystable Fano varieties and reduce the problem to a conjecture on destabilizations of K-unstable Fano varieties. Specifically, we prove that if the stability threshold of every K-unstable Fano variety is computed by a divisorial valuation, then such K-moduli spaces are proper. The argument relies on studying certain optimal destabilizing test configurations and constructing a Θ -stratification on the moduli stack of Fano varieties. [ABSTRACT FROM AUTHOR]