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Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea.
- Source :
- Communications Biology; 10/20/2022, Vol. 5 Issue 1, p1-11, 11p
- Publication Year :
- 2022
-
Abstract
- Methanogens and anaerobic methane-oxidizing archaea (ANME) are important players in the global carbon cycle. Methyl-coenzyme M reductase (MCR) is a key enzyme in methane metabolism, catalyzing the last step in methanogenesis and the first step in anaerobic methane oxidation. Divergent mcr and mcr-like genes have recently been identified in uncultured archaeal lineages. However, the assembly and biochemistry of MCRs from uncultured archaea remain largely unknown. Here we present an approach to study MCRs from uncultured archaea by heterologous expression in a methanogen, Methanococcus maripaludis. Promoter, operon structure, and temperature were important determinants for MCR production. Both recombinant methanococcal and ANME-2 MCR assembled with the host MCR forming hybrid complexes, whereas tested ANME-1 MCR and ethyl-coenzyme M reductase only formed homogenous complexes. Together with structural modeling, this suggests that ANME-2 and methanogen MCRs are structurally similar and their reaction directions are likely regulated by thermodynamics rather than intrinsic structural differences. A heterologous protein expression platform in a culturable methanogen Methanococcus maripaludis is developed to study the methyl-coenzyme M reductase from uncultured archaea. [ABSTRACT FROM AUTHOR]
- Subjects :
- OPERONS
CARBON cycle
ARCHAEBACTERIA
REDUCTASES
ENZYME metabolism
PROTEIN expression
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 5
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Communications Biology
- Publication Type :
- Academic Journal
- Accession number :
- 159793847
- Full Text :
- https://doi.org/10.1038/s42003-022-04057-6