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Invertebrate species are a natural reservoir of viral genetic diversity, and invertebrate pests are widely distributed in crop fields. However, information on viruses infecting invertebrate pests of crops is limited. In this report, we describe the deep metatranscriptomic sequencing of 88 invertebrate samples covering all major invertebrate pests in rice fields. We identified 296 new RNA viruses and 13 known RNA viruses. These viruses clustered within 31 families, with many highly divergent viruses constituting potentially new families and genera. Of the identified viruses, 13 RNA viruses clustered within the Fiersviridae family of bacteriophages, and 48 RNA viruses clustered within families and genera of mycoviruses. We detected known rice viruses in novel invertebrate hosts at high abundances. Furthermore, some novel RNA viruses have genome structures closely matching to known plant viruses and clustered within genera of several plant virus species. Forty-five potential insect pathogenic RNA viruses were detected in invertebrate species. Our analysis revealed that host taxonomy plays a major role and geographical location plays an important role in structuring viral diversity. Cross-species transmission of RNA viruses was detected between invertebrate hosts. Newly identified viral genomes showed extensive variation for invertebrate viral families or genera. Together, the large-scale metatranscriptomic analysis greatly expands our understanding of RNA viruses in rice invertebrate species, the results provide valuable information for developing efficient strategies to manage insect pests and virus-mediated crop diseases. [ABSTRACT FROM AUTHOR]

This article reports the first detection of papaya cytorhabdovirus (PCRV) in soybean plants in Zhejiang province, China. The soybean plants exhibited symptoms such as shrinkage, yellow spots, and chlorotic spots with yellowing. Through RNA extraction and sequencing, a contig with high nucleotide identity to PCRV was identified. RT-PCR assays confirmed the presence of PCRV in 9 out of 12 symptomatic soybean samples. This finding is significant as PCRV has previously been found in other hosts and monitoring for the virus and its vector host will be important for controlling its spread in China. The research was supported by the National Natural Science Foundation of China. [Extracted from the article]

Keywords: Chickpea chlorotic stunt virus (CpCSV); Weed; Viruses EN Chickpea chlorotic stunt virus (CpCSV) Weed Viruses 1155 1156 2 09/15/23 20230801 NES 230801 Chickpea chlorotic stunt virus (CpCSV), a member of the genus I Polerovirus i of the family I Solemoviridae i , has been described in Ambo, Ethiopia (AY956384) and Yunnan, China (JF507725). The contig was 5,883 bp in size with an average sequencing coverage of 1721x, and showed 87.35% and 87.48% nucleotide identities with CpCSV isolates from Ethiopia and China, respectively. [Extracted from the article]