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Kombucha beverage produced through fermentation of sugared tea using bacteria and yeast has gained attention for its beneficial health benefits. However, the cost linked to the raw materials often increases the upstream process expenses, thereby the overall operating expenditures. Thus, there is a need to explore alternative waste and cost-effective raw materials for Kombucha fermentation. The present study, compared the physico-chemical and microbial growth pattern of Kombucha beverage production using tea waste from the tea processing industries with that of the green/black tea, reporting similar trends irrespective of its type. Further, the amplicon sequencing of 16S rRNA showed dominant presence of Komagataeibacter rhaeticus and high throughput sequencing of ITS1 confirmed the presence of yeast species similar to Brettanomyces bruxellensis in the tea waste based Kombucha beverage. Appreciable amount of carbohydrates (8.5/100 g) and energy (34 kcal/100 g) with appropriate organoleptic properties favourable for human consumption were also observed during the nutritional content and qualitative property assessment. The overall study showed a broad taxonomic and functional diversity existing during Kombucha fermentation process with tea waste to maintain a sustained eco-system to facilitate cost-effective beverage production with desired properties for safe consumption. [ABSTRACT FROM AUTHOR]

Scale-up and commercialization of biodiesel is often delimited by costly feedstock that adds up to the process costs. These underlying issues demand the exploration of unconventional cheap feed to improve the process economics. Conversion of waste cooking oil (WCO) into biodiesel could reduce the process costs by 60–70%. However, the continuous exposure to heat during frying leads to oxidation as well increase in the free fatty acid (FFA) content which intensifies the time and energy required for transesterification. The present study analyzes the effect of parameters over the conversion of WCO (with 8.17% FFA) into biodiesel via two-step acid-alkali-based microwave-assisted transesterification. Response surface methodology (RSM) was used to optimize the oil:methanol volume ratio, microwave power, and reaction time during the acid-catalyzed esterification to bring down the FFA below 1%. Microwave irradiation of 250 W, with methanol:oil molar ratio of 19.57:1 [oil:methanol volume ratio of 1.31 (expressed as decimal)] and reaction time of 35 s, resulted in 0.082% of FFA. Alkali-catalyzed transesterification with methanol:oil molar ratio of 5:1 with 2% sodium hydroxide at 65 °C thereby produced fatty acid methyl esters (FAMEs) with the volumetric biodiesel yield of 94.6% in 30 min. Physiochemical properties of the transesterified WCO were well comparable with the biodiesel standards. The study highlights the essentiality of multivariate optimization for the esterification process that could aid in understanding the interactive effects of variables over FFA content. Such studies would benefit in scaling up of the transesterification process at industrial level by improving the economics of the overall bioprocess. [ABSTRACT FROM AUTHOR]